Milking system and diet's forage type impact on milk quality of Italian Holstein-Friesian.

Moving from conventional (CMS) to automatic (AMS) milking systems could impact milk quality. Moreover, the type and preservation methods of the forages used in the total mixed ration (TMR) (such as alfalfa hay -HTMR- or corn silage -STMR-) have been demonstrated to modify milk composition. Thus, this study investigated the effect of implementing AMS and different diet forage types on the quality of Italian Holstein-Friesian bulk milk. Milk samples (n = 168) were collected monthly from 21 commercial farms in northern Italy during a period of 8 mo. Farms were categorized into 4 groups according to their milking system (CMS vs AMS) and diet's forage type (HTMR vs STMR). Milk quality data were analyzed through the mixed procedure for repeated measurement of SAS with the milking system, diet's forage type, and sampling day as fixed effects. Milking through the AMS led to lower milk fat, freezing point and ß-lactoglobulin A, longer coagulation time, and higher K content, pH and ß-lactoglobulin B than CMS. Cows fed STMR produced milk with greater fat, protein, casein, Mg content, titratable acidity and ß-lactoglobulin A, while reduced curd firming time, freezing point and ß-lactoglobulin B than those fed with HTMR. In conclusion, milk quality is not only altered by the diet's forage type and characteristics but also by the milking system.

Impact of 5-20% Hydroponic Wheat Sprouts Inclusion on Growth and Metabolic Parameters of Growing Ewes.

The aim of this study was to assess the impact of varying proportions (5-20%) of hydroponic wheat sprouts in the diet of growing four-month-old Hu ewes on their productive performance, metabolic profiles, rumen fermentation, and alterations in microflora. Compared with the control group (CON), the optimum final weight of ewes has been presented in the group of substitution 15% (S15) of the basal diet with hydroponic wheat sprouts. Furthermore, 1-30 d the average daily gain (ADG), 31-60 d ADG, and average feed intake were both significantly improved in S15 compared with CON (p < 0.05). Feeding hydroponic wheat sprouts can significantly increase high-density lipoprotein and interleukin-2 (p < 0.05) accompanied by the numerical increase of the content of interferon-γ, suggesting its positive effect on ewes' health and immune systems. In this process, it is noteworthy that feeding hydroponic wheat sprouts results in an increase in relative abundance of Olsenella, Limosilactobacillus, Shuttleworthia, and Prevotella_7, and a decrease in relative abundance of Succinimonas, Pseudobutyrivibrio, and Anaerovibrio in the rumen of growing ewes. It implies that the response of rumen microflora adapted to the change of dietary ingredients, as well as the relationship between rumen microflora changes and the improvement of productive performance and immune system in growing ewes. Considering the usage cost and application effect, S15 of the basal diet with hydroponic wheat sprouts could be the appropriate application solution for growing ewes.

Study on Modeling and Evaluating Alfalfa Yield and Optimal Water Use Efficiency in the Agro-Pastoral Ecotone of Northern China.

The agro-pastoral ecotone in northern China is the main production area of agriculture and animal husbandry, in which agricultural development relies entirely on groundwater. Due to the increasing water consumption of groundwater year by year, groundwater resources are becoming increasingly scarce. The substantial water demand and low germination rate in the first year are the main characteristics of alfalfa (Medicago sativa L.) yield in the agro-pastoral ecotone in northern China. Due to unscientific irrigation, water resources are seriously wasted, which restricts the development of local agriculture and animal husbandry. The study constructed the Dssat-Forages-Alfalfa model and used soil water content, leaf area index, and yield data collected with in situ observation experiments in 2022 and 2023 to calibrate and validate the parameters. The study found ARE < 10%, ENRMS < 15%, and R2 ≥ 0.85. The model simulation accuracy was acceptable. The study revealed that the water consumption at the surface soil layer (0-20 cm) was more than 6~12% and 13~31% than that at the 20-40 cm and 40-60 cm soil layers, respectively. The study showed when the irrigation quota was 30 mm, the annual yield of alfalfa (Medicago sativa L.) (7435 kg/ha) was consistent with that of the irrigation quota of 33 mm, and increased by 3.99% to 5.34% and 6.86% to 10.67% compared with that of irrigation quotas of 27 mm and 24 mm, respectively. To ensure the germination rate of alfalfa (Medicago sativa L.), it is recommended to control the initial soil water content at 0.8 θfc~1.0 θfc, with an irrigation quota of 30 mm, which was the best scheme for water-use efficiency and economic yield. The study aimed to provide technological support for the rational utilization of groundwater and the scientific improvement of alfalfa yield in the agro-pastoral ecotone in northern China.

Root exudate fingerprint of Brachiaria humidicola reveals vanillin as a novel and effective nitrification inhibitor.

Introduction: Biological Nitrification Inhibition (BNI) is defined as the plant-mediated control of soil nitrification via the release of nitrification inhibitors. BNI of Brachiaria humidicola (syn. Urochloa humidicola) has been mainly attributed to root-exuded fusicoccane-type diterpenes, e.g., 3-epi-brachialactone. We hypothesized, however, that BNI of B. humidicola is caused by an assemblage of bioactive secondary metabolites. Methods: B. humidicola root exudates were collected hydroponically, and metabolites were isolated by semi-preparative HPLC. Chemical structures were elucidated by HRMS as well as 1D and 2D NMR spectroscopy. Nitrification inhibiting potential of isolated metabolites was evaluated by a Nitrosomonas europaea based bioassay. Results and discussion: Besides previously described brachialactone isomers and derivatives, five phenol and cinnamic acid derivatives were identified in the root exudates of B. humidicola: 2-hydroxy-3-(hydroxymethyl)benzaldehyde, vanillin, umbelliferone and both trans- and cis-2,6-dimethoxycinnamic acid. Notably, vanillin revealed a substantially higher nitrification inhibiting activity than 3-epi-brachialactone (ED50 ∼ 12.5 µg·ml-1, ED80 ∼ 20 µg·ml-1), identifying this phenolic aldehyde as novel nitrification inhibitor (NI). Furthermore, vanillin exudation rates were in the same range as 3-epi-brachialactone (1-4 µg·h-1·g-1 root DM), suggesting a substantial contribution to the overall inhibitory activity of B. humidicola root exudates. In relation to the verification of the encountered effects within soils and considering the exclusion of any detrimental impact on the soil microbiome, the biosynthetic pathway of vanillin via the precursor phenylalanine and the intermediates p-coumaric acid/ferulic acid (precursors of further phenolic NI) might constitute a promising BNI breeding target. This applies not only to Brachiaria spp., but also to crops in general, owing to the highly conserved nature of these metabolites.

Chemical Composition and In Vitro Ruminal Fermentation Characteristics of Native Grasses from the Floodplain Lowlands Ecosystem in the Colombian Orinoquia.

Grasses from lowland ecosystems in flooded savannahs are useful to feed extensive grazing animals; however, scarce information about its agronomic and fermentation characteristics exists. This study aims to determine the chemical composition and fermentation parameters of native grasses from the floodplain lowlands ecosystem in the Colombian Orinoquia. Three native grasses (Leersia hexandra, Acroceras zizanioides and Hymenachne amplexicaulis) and a "control" grass (introduced Urochloa arrecta-Tanner grass) were sown and sampled at 30, 40 and 50 days of age. On each sampling date, biomass production in a 1 m2 frame was estimated, and the chemical composition and fermentation parameters were analyzed using near-infrared spectroscopy and the in vitro gas production technique, respectively. Data were analyzed using a mixed model for repeated measures and the least significant difference (LSD) was used for mean differentiation (p < 0.05). The grasses' nutritional characteristics varied as follows: dry matter (DM, 0.7-2.0 ton/ha), crude protein (CP, 6.1-12.2%), neutral detergent fiber (NDF, 56.6-69.6%), ash (5.8-15.8%) and dry matter digestibility (DMD) between 20.8 and 60.6% from 12 to 48 h of fermentation. Native plants such as L. hexandra and A. zizanioides presented higher biomass production, CP, ash, cellulose, and Ca levels than the control plant. During the experimental period (30 to 50 days), the grasses did not present significant nutrient availability changes. In terms of fermentation characteristics, L. hexandra increased ammonia concentrations and total volatile fatty acids (TVFA) and butyric acid. This latter effect was also observed in A. zizanioides grass. L. hexandra and A. zizanioides grasses constitute a valuable alternative forage resource during the flooding times of the studied ecosystem.

The diversity and quality of forages and their potency as herbal anthelmintic for swamp buffalo in Brebes District, Central Java.

Background and Aim: Swamp buffaloes play an important role in the rural economy of Indonesia. They consume various forages during their grazing time, including those with anti-parasitic potential. However, the information about the type and quality of forages and their potential as a natural anthelmintic for swamp buffalo is very limited. This study aimed to identify the diversity, quality, and anthelmintic potency of forages consumed by swamp buffaloes reared in Bantarkawung Subdistrict, Brebes District, Central Java Province, Indonesia. Materials and Methods: Samples of forages were obtained during three observation periods of the study, with a 12-week interval between each period. Forage diversity was evaluated by identifying its consumption by swamp buffaloes during their grazing activity in the field and feeding time in their shed. The quality of forages was analyzed using proximate analysis to measure their dry matter (DM), crude fiber (CF), crude protein (CP), crude fat (CFat), and ash contents. This is followed by the calculation of their total digestible nutrient based on the proximate analysis results. Botanical composition analysis was then conducted to measure the predominance of forages consumed by the livestock during their grazing activity. Literature reviews were carried out to explore forage's anthelmintic activity. Results: The results showed that swamp buffaloes consume nine species of forage in the shed and 47 in the grazing area, including nine legumes, 18 grass, and 20 others. Swamp buffaloes consumed forages of lower quality, which contained high CF contents and varying levels of other nutrients below their daily nutritional needs. The grazing activity allowed swamp buffaloes to consume a higher variety of forages with better nutritional quality, thereby enabling them to meet their nutritional needs. Legumes and other forages served as the major protein sources, providing CP of 20.03% DM and 11.53% DM, and CF levels of 17.01% DM and 20.35% DM, respectively. The results also showed that the consumption of these forages increased during the rainy season. The predominant species of legumes consumed were Leucaena leucocephala and Acacia spp., while Alternanthera sessilis and Merremia umbellata were the predominant species of other forages. A total of 13 of the 47 species could potentially be used as natural anthelmintic due to their secondary metabolites, namely, tannin, flavonoid, saponin, terpenoid, diterpenoid, and mimosine. These compounds exert anthelmintic effects by inhibiting egg-hatching and larval development, as well as damaging the surface structure of both larvae and adult worms, ultimately leading to the death of the parasites. Conclusion: Overall, swamp buffaloes consumed more variety of forages during grazing compared to when they were kept in sheds. While the low-variety and low-quality forages provisioned for swamp buffaloes in their shed resulted in a low nutrient intake below their daily requirement. Furthermore, daily grazing activities allowed swamp buffaloes to fulfill and supplement their need by consuming a variety of grasses, legumes, and other forages in their respective grazing areas. Some of these forages also have the potential to become natural anthelmintic because they contain secondary metabolites, such as tannins, flavonoids, saponins, terpenoids, diterpenoids, and mimosine.

Evaluation of trace elements in forages and their effect on gastrointestinal parasite burden in grazing sheep.

This study was designed to evaluate the trace elements (minerals) in forages fed to sheep and their effect on gastrointestinal parasite burdens. The ultimate objective was to determine the correlation between the burden of gastrointestinal (GI) parasites and the level of trace minerals in sheep serum as a result of the forages they grazed on. A total of 384 faecal samples were collected from sheep in each of the districts (Sialkot and Multan) and examined quantitatively using the McMaster technique. Serum collected from them and plants were pre-treated, and spectrophotometry was used to determine the concentration of trace minerals (Mn, Co, Cu, and Zn). The level of these trace elements differed significantly (P < 0.05) in forages from both districts. In the district of Sialkot, the highest concentrations (mg/Kg) of Zn (38.53 ± 0.16) were found in Cichorium intybus, Cu (41.57 ± 0.07) in Cynodon dactylon, Mn (39.61 ± 0.05) in Parthenium hysterophorus, and Co (1.42 ± 0.03) in Coronopus didymus. In the district of Multan, the highest concentrations (mg/Kg) of Zn (39.43 ± 0.46) were found in Cichorium intybus, Cu (25.76 ± 0.36) in Cynodon dactylon, Mn (34.29 ± 0.53) in Launaea nudicaulis, and Co (1.74 ± 0.08) in Brachiaria raptens. The prevalence of GI parasites in sheep populations in district Sialkot was 34%, while in district Multan, it was 32%. In tehsil Sialkot of district Sialkot, Zn and Cu were significantly (P < 0.05) correlated with eggs per gram (EPG) of faeces, while in tehsil Multan City of district Multan, only Cu was significantly (P < 0.05) correlated with EPG. The potential mechanism behind the role of trace minerals in lowering the burdens of GI parasites requires more investigation. It is recommended that plants with high content of trace minerals should be utilized as part of comprehensive preventive and control strategies against GI parasitism in ruminant animals like sheep.

Dietary Ruminant Enteric Methane Mitigation Strategies: Current Findings, Potential Risks and Applicability.

This review examines the current state of knowledge regarding the effectiveness of different dietary ruminant enteric methane mitigation strategies and their modes of action together with the issues discussed regarding the potential harms/risks and applicability of such strategies. By investigating these strategies, we can enhance our understanding of the mechanisms by which they influence methane production and identify promising approaches for sustainable mitigation of methane emissions. Out of all nutritional strategies, the use of 3-nitrooxypropanol, red seaweed, tannins, saponins, essential oils, nitrates, and sulfates demonstrates the potential to reduce emissions and receives a lot of attention from the scientific community. The use of certain additives as pure compounds is challenging under certain conditions, such as pasture-based systems, so the potential use of forages with sufficient amounts of plant secondary metabolites is also explored. Additionally, improved forage quality (maturity and nutrient composition) might help to further reduce emissions. Red seaweed, although proven to be very effective in reducing emissions, raises some questions regarding the volatility of the main active compound, bromoform, and challenges regarding the cultivation of the seaweed. Other relatively new methods of mitigation, such as the use of cyanogenic glycosides, are also discussed in this article. Together with nitrates, cyanogenic glycosides pose serious risks to animal health, but research has proven their efficacy and safety when control measures are taken. Furthermore, the risks of nitrate use can be minimized by using probiotics. Some of the discussed strategies, namely monensin or halogenated hydrocarbons (as pure compounds), demonstrate efficacy but are unlikely to be implemented widely because of legal restrictions.

Vitamin and Trace Element Nutrition of Stocker Cattle on Small Grain and Winter Annual Pastures.

This article reviews the trace mineral and macro mineral content of small grain forages and the potential role in the health of cattle grazing the forages. Reasons for the variability of trace mineral content in small grain forages are discussed, as well as the role of antagonists, such as sulfur and molybdenum, in creating trace mineral deficiencies. The sampling of cattle for the determination of trace mineral statues is described, including which samples to collect for analysis, as well as sample handling. The authors offer a useful discussion on the vitamin content of small grain forages, and conclude that vitamin supplementation is not necessary.

Assessment of different dietary alfalfa hay to alfalfa silage ratios for dromedary camel feeding.

Two in vivo experiments studied the effects of different alfalfa hay (AH) to alfalfa silage (AS) ratios including 100:0 (AH100), 50:50 (AH50:AS50) and 0:100 (AS100) in total mixed rations (TMR) of dromedary camels. In experiment (Exp.) 1, a total of 18 multiparous Baluchi dairy camel [100 ± 5 days in milk (DIM); 3.65 ± 0.539 kg milk yield] were randomly allocated to one of the three groups (n = 6) for 42 d of experimental period. Dry matter intake (DMI) and milk yield were recorded daily, and blood samples were collected on days 0, 21 and 42. In Exp. 2, 18 male Baluchi camel calves [275 ± 14 days of old; 105 ± 8 kg BW] were housed in individual shaded pens for 150 days. DMI was recorded daily and individual weights of camels were recorded monthly. Blood samples were collected on days 0, 75 and 150. In Exp.1, feeding different dietary AH:AS ratios altered neither DMI (p = 0.351) nor milk yield (p = 0.667). Of all milk components, only the milk urea nitrogen (MUN) was increased (p = 0.015) by AS feeding (both AH50:AS50 and/or AS100). AS feeding tended to increase AST (p = 0.099) and ALT (p = 0.092) levels in lactating camels. In Exp. 2, DMI (p = 0.845), average daily gain (ADG; p = 0.092) and return per kg BW gain (p = 0.710) of silage-fed camels were similar to those of hay-fed group. The plasma concentration of BUN (p = 0.014) and AST (p = 0.014) were increased in camels fed AS100. Overall, the results suggest that both AS and/or AH could be used in dromedary camel diets based on the climatic condition, season and available facilities; however, the long-term use of AS (as sole forage) should done with caution due to the potential risk of impaired liver function. Further studies needed to explore the impact of hay versus silage feeding on digestibility, rumen function and nitrogen pollution in camel feeding.

Multi-allelic gene editing in an apomictic, tetraploid turf and forage grass (Paspalum notatum Flüggé) using CRISPR/Cas9.

Polyploidy is common among grasses (Poaceae) and poses challenges for conventional breeding. Genome editing technology circumvents crossing and selfing, enabling targeted modifications to multiple gene copies in a single generation while maintaining the heterozygous context of many polyploid genomes. Bahiagrass (Paspalum notatum Flüggé; 2n=4x=40) is an apomictic, tetraploid C4 species that is widely grown in the southeastern United States as forage in beef cattle production and utility turf. The chlorophyll biosynthesis gene magnesium chelatase (MgCh) was selected as a rapid readout target for establishing genome editing in tetraploid bahiagrass. Vectors containing sgRNAs, Cas9 and nptII were delivered to callus cultures by biolistics. Edited plants were characterized through PCR-based assays and DNA sequencing, and mutagenesis frequencies as high as 99% of Illumina reads were observed. Sequencing of wild type (WT) bahiagrass revealed a high level of sequence variation in MgCh likely due to the presence of at least two copies with possibly eight different alleles, including pseudogenes. MgCh mutants exhibited visible chlorophyll depletion with up to 82% reductions in leaf greenness. Two lines displayed progression of editing over time which was linked to somatic editing. Apomictic progeny of a chimeric MgCh editing event were obtained and allowed identification of uniformly edited progeny plants among a range of chlorophyll depletion phenotypes. Sanger sequencing of a highly edited mutant revealed elevated frequency of a WT allele, probably due to frequent homology-directed repair (HDR). To our knowledge these experiments comprise the first report of genome editing applied in perennial, warm-season turf or forage grasses. This technology will accelerate bahiagrass cultivar development.

Ensiling Characteristics, In Vitro Rumen Fermentation Patterns, Feed Degradability, and Methane and Ammonia Production of Berseem (Trifolium alexandrinum L.) Co-Ensiled with Artichoke Bracts (Cynara cardunculus L.).

This study investigated the effect of co-ensiling increasing levels of artichoke bracts (Cynara cardunculus L.) with berseem (Trifolium alexandrinum L.) (100:0, 75:25, 50:50, 25:75, and 0:100, respectively) on silage quality after 0, 30, 60, and 120 days. Moreover, the in vitro rumen fermentation characteristics and methane (CH4) and ammonia (NH3-N) production were evaluated using a buffalo inoculum source. The results showed that pH of the silage and the concentration of acetic, propionic, butyric acid, and NH3-N significantly decreased (L; p < 0.01) with the increasing amounts of artichoke bracts in the mixture. At 30 and 60 days of ensiling, the highest lactic acid concentration was observed at intermediate proportions of artichoke bracts (p < 0.01). Cumulative gas production was higher in artichoke bracts than in the berseem silage. After 24 h of incubation, the highest value (p < 0.05) of truly dry matter, organic matter, natural detergent fiber degradability, and NH3-N concentration was recorded with 500 g/kg of forage mixtures. As the artichoke bract concentration increased, the partitioning factor and ruminal pH declined linearly (p ≤ 0.05). No significant differences were observed for total volatile fatty acids and volatile fatty acids molar proportions. In summary, co-ensiling artichoke bracts with berseem at a ratio of 1:1 might be a promising and easy method for the production of high-quality silage from legume forage with positively manipulating rumen fermentation.

Identification of Ruminal Fermentation Curves of Some Legume Forages Using Particle Swarm Optimization.

The modeling process has a wide range of applications in animal nutrition. The purpose of this work is to determine whether particle swarm optimization (PSO) could be used to explain the fermentation curves of some legume forages. The model suited the fermentation data with minor statistical differences (R2 > 0.98). In addition, reducing the number of iterations enhanced this method's benefits. Only Models I and II could successfully fit the fermentability data (R2 > 0.98) in the vetch and white clover fermentation curve because the negative parameters (calculated in Models III and IV) were not biologically acceptable. Model IV could only fit the alfalfa fermentation curve, which had higher R values and demonstrated the model's dependability. In conclusion, it is advised to use PSO to match the fermentation curves. By examining the fermentation curves of feed materials, animal nutritionists can obtain a broader view of what ruminants require in terms of nutrition.

Potential Forage Hybrid Markets for Enhancing Sustainability and Food Security in East Africa.

The cattle sector is strategic for both the economic development and food security of Africa, but the low availability and quality of forage puts the most vulnerable population at risk. Hybrid forages are an alternative for enhancing both food security and sustainability of the sector but adoption levels are still low in Africa, which is related to various factors such as the availability of seeds. This document analyzes potential markets for new interspecific hybrids of Urochloa and potential hybrids of Megathyrsus maximus, adapted to the environmental conditions of eastern and partially western Africa, applying a four-step methodology based on estimating (i) required forage amounts for each country according to its dairy herd, (ii) potential hectares for forage cultivation based on (i), (iii) hectares that can be covered by the two hybrids of interest according to a Target Population of Environment approach, and (iv) potential market values for each country and hybrid. The results show a potential market of 414,388 ha for new interspecific hybrids of Urochloa and 528,409 ha for potential hybrids of Megathyrsus maximus, with approximate annual values of 73.5 and 101.1 million dollars, respectively. Ethiopia, Tanzania, and Kenya hold a market share of 70% for Urochloa, and South Sudan, Ethiopia, and Tanzania a 67% market share for Megathyrsus maximus. The results will help different actors in decision-making, i.e., regarding private sector investments in forage seed commercialization or public sector incentives supporting adoption processes, and thus contribute to increasing food security and sustainability in the region.

Use of near-infrared reflectance spectroscopy on feces to estimate digestibility and dry matter intake of dietary nutritional characteristics under grazing conditions in Colombian creole steers.

Digestibility and intake are parameters difficult and expensive to estimate under grazing conditions; therefore, the aim of this study was to develop near-infrared reflectance spectroscopy (NIRS) calibrations applied to feces (F-NIRS) and evaluate their accuracy to predict dry matter digestibility (DMD) and dry matter intake (DMI) of Colombian creole cattle. Five digestibility trials using creole steers were conducted; indigestible neutral detergent fiber (iNDF) was used as internal marker and Cr2O3 and TiO2 as external markers. A total of 249 forage and 396 fecal samples from individual animals were collected, dried, and grinded for conventional chemical analysis. For spectral analysis, fecal samples were pooled across collection periods (77 samples). Chemometric analysis was performed using WinISI V4.10 software applying the modified partial least squares method. Cross-validation was performed to avoid overfitting the models. The goodness-of-fit statistics considered were the coefficient of determination in cross-validation and prediction sets (R2cv and r2, respectively) and the ratio performance deviation (RPD). Fecal NIRS calibrations developed for forage and supplement DMD showed a satisfactory fit (R2cv =0.87 and RPD=2.77 and R2cv=0.92 and RPD=3.50, respectively). The accuracy of fecal output equations using chromium (Cr) and titanium (Ti) was similar in terms of R2cv (0.92) and RPD (3.63 vs. 3.57). Total DMI equations using Ti performed better compared to Cr (R2cv = 0.82 vs. 0.78; RPD=2.41 vs. 2.17, respectively). The F-NIRS models were validated using a completely independent set of fecal samples showing a moderate fit (r2>0.8 and RPD>2.0). This study showed that F-NIRS is a feasible tool to predict DMD and DMI of creole steers under grazing conditions. However, previous to socialization, this requires an improvement in accuracy of the calibrated equations related to grazing animals in different production contexts.

Reproductive and Agronomic Characterization of Novel Apomictic Hybrids of Paspalum (Poaceae).

The tetraploid germplasm of Paspalum contains a large diversity that can be used to generate better forages. The objective was to evaluate a group of Paspalum notatum and Paspalum simplex apomictic hybrids for a set of agronomic traits and apomixis expressivity. Forage yield, cold tolerance, winter regrowth, and seed yield were evaluated. The expressivity of apomixis was evaluated in P. simplex hybrids by flow cytometry. Progeny testing with molecular markers was used to determine the genotypic variability in the progeny. Differences within P. notatum and P. simplex hybrids were observed for all traits, and some of them were superior in comparison with the controls. The accumulated forage yield during three years was 988 g m-2 in the P. notatum hybrids, whereas, in P. simplex, the average forage yield per harvest (40 days of regrowth) was 180 g m-2. In P. simplex, the apomixis expressivity varied between 0 and 100%, and 65% of the hybrids showed high apomixis expressivity (superior to 70%). The genotypic mean homogeneity in the progeny was 76% and 85% in P. notatum and P. simplex, respectively. The generation of hybrids with high apomixis expressivity that combine good agronomic performance and homogeneity in the offspring is possible in tetraploid P. notatum and P. simplex.

Nitrogen fertilization increases the growth and nutritional quality of the forage legume, Calobota sericea - A preliminary investigation.

Calobota sericea is being evaluated as a forage for drought stressed areas. The nutritional quality of C. sericea from native populations are poor, and this is believed to be due to poor soil fertility. Therefore, a greenhouse trial was established to determine the impact of N-fertilization level (0, 25, 50, 75 and 100 kg/ha) on the growth and nutritional quality of C. sericea plants. Three-month-old plants were harvested and the root and shoot length, as well as branching intensity on each plant determined. Thereafter, the shoots were separated into leaves and stems and all plant parts were oven dried for dry mass determination. After weighing, the leaves and stems for each plant were combined and the dried shoots used for nutrient determination. Results indicated that increased N application levels is positively correlated with improved C. sericea growth. Similarly, mineral nutrient uptake increased significantly under all the N-fertilization treatments and crude protein content increased from 9.6% to 18.6%. Plant growth was only statistically significantly (p < 0.05) improved when N was applied at rates of 50 kg/ha and more, but crude protein content increased from the lowest N application rates (25 kg/ha). The improved growth and nutrient uptake could primarily be explained by improved resource allocation under N-fertilization. Therefore, appropriately fertilized C. sericea can result in improved forage production and improved quality forages and when N is applied at high enough rates.

Building comprehensive glucosinolate profiles for brassica varieties.

Brassica plants play an important role in common agricultural practices, such as livestock feed or biofumigation, due to the bioactivity of the natural degradation products of glucosinolate metabolites. Therefore, the ability to survey comprehensive glucosinolate profiles for individual brassicas is essential for informing proper species selection for the intended application. Current methods for glucosinolate identification and quantification involve complex or unconventional procedures, and proper reference materials are not readily available. Therefore, researchers with limited resources that require glucosinolate profiles are at an extreme disadvantage. In this work, a simple and accurate HPLC-MS method was developed and validated to build preliminary glucosinolate profiles for three agriculturally relevant forage brassica varieties [turnip (B. rapa L.), canola (B. napus L.), and rapeseed (B. napus L.)]. The average glucosinolate content across three herbage collection dates for canola, rapeseed and turnip were 2.9 ± 0.9 mg g-1, 6.4 ± 1.3 mg g-1, and 14 ± 3.4 mg g-1, respectively. GLS concentrations are reported in milligrams of glucosinolate, calculated as sinigrin equivalents, per gram of dry plant material. This semi-quantitative approach for reporting total GLS content in brassicas is accurate within 15%. Several minor individual glucosinolates were identified that have not been previously reported in canola, rapeseed and turnip species, including glucotropaeolin and 4-hydroxyglucobrassicin (canola), glucoraphanin and glucoberteroin (rapeseed), and glucosinalbin and glucobarbarin (turnip). This non-targeted screen of several forage brassica varieties demonstrates the inherent variation in both the individual glucosinolate content and the total glucosinolate profile among brassicas, and highlights the importance of such glucosinolate characterization in agricultural practices. Additionally, the method developed in this study can be used as a tool for researchers with limited resources to build accurate glucosinolate profiles of brassica plants.

Effects of Hay, Baleage, and Soybean Hulls Waste Used as Supplemental Feeds on the Nutritional Profile of Grass-Finished Beef.

Grass-finished beef (GFB) has demonstrated wide nutritional variations with some GFB having a considerably higher n-6:n-3 ratio compared to grain-finished beef. To better understand these variations, the current study investigated the effects of commonly used supplemental feeds on the nutritional profile of GFB. This two-year study involved 117 steers randomly allocated to one of four diets: (1) grass+hay (G-HAY), (2) grass+baleage (G-BLG), (3) grass+soybean hulls (G-SH), and (4) baleage+soybean hulls in feedlot (BLG-SH). Feed samples were analyzed for their nutritional value, and beef samples underwent analysis for fatty acids (FAs), vitamin E, minerals, lipid oxidation, and shear force. FAs were measured by GC-MS, vitamin E was analyzed chromatographically, minerals were analyzed by ICP-MS, and lipid oxidation was measured via a thiobarbituric acid reactive substances (TBARS) assay. G-SH beef had the highest n-6:n-3 ratio (p < 0.001), while BLG-SH beef contained less vitamin E (p < 0.001) and higher TBARS values (p < 0.001) compared to the other groups. G-HAY beef contained more long-chain n-3 polyunsaturated FAs compared to the other groups (p < 0.001). In conclusion, G-HAY beef had the most beneficial nutritional profile, while soybean hulls increased the n-6:n-3 ratio of beef.