RESUMO
Objective To establish human brain-derived neurotrophic factor (BDNF) genetical modified marrow stromal cells and to evaluate the influence on the viability of the cultured spiral ganglion cells from Kunming mice.Methods Eukaryotic expression plasmid——pcDNA3.1(-)-BDNF was constructed according to the molecular clone approach. Marrow stromal cells(MSC) were separated and cultured. BDNF genetical modified marrow stromal cells were established and verified by biochemical analysis.The bio-effect to spiral ganglion cells, especially with the oxidative damage under the different concentration of H_2O_2 was observed.Results PcDNA3.1(-)-BDNF and BDNF genetical engineering cells were successfully established. The supernatant from BDNF-MSCs remarkably improved the survival rate of the spiral ganglion cells.Conclusion BDNF genetical modified marrow stromal cells are successfully established, and the genetical engineering cell plays an important role in the protection the spiral ganglion cells from the oxidative damage. This study provide a strong basis for genetical engineering cells transplantation into the inner ear.
