Artificial Intelligence, the Production of Scientific Texts, and the Implications for Sleep Science: Exploring Emerging Paradigms and Perspectives.

The emergence of artificial intelligence (AI) has revolutionized many fields, including natural language processing, and marks a potential paradigm shift in the way we evaluate knowledge. One significant innovation in this area is ChatGPT, a large language model based on the GPT-3.5 architecture created by OpenAI, with one of its main aims being to aid in general text writing, including scientific texts. Here, we highlight the challenges and opportunities related to using generative AI and discuss both the benefits of its use, such as saving time by streamlining the writing process and reducing the amount of time spent on mundane tasks, and the potential drawbacks, including concerns regarding the accuracy and reliability of the information generated and its ethical use. In respect of both education and the writing of scientific texts, clear rules and objectives and institutional principles must be established for the use of AI. We also consider the positive and negative effects of the use of AI technologies on interpersonal interactions and behavior, and, as sleep scientists, its potential impacts on sleep. Striking a balance between the benefits and potential drawbacks of integrating AI into society demands ongoing research by experts, the wide dissemination of the scientific results, as well as continued public discourse on the subject.

Modified Surgical Guide for Root Sectioning in the Socket Shield Technique-In Vitro Study.

OBJECTIVE: This in vitro study aimed to evaluate the effectiveness of a printed surgical guide for root sectioning in the socket-shield technique. MATERIALS AND METHODS: A typodont model of the maxilla with augmented filler was used for the upper right central incisor, and CBCT images were obtained. Two types of vertical root sectioning guides were tested: one with a buccal sleeve (Group A, n = 10) and another with a buccal orientation slit (Group B, n = 10). Control group (n = 10) performed freehand cuts on printed models. After crown cutting with diamond burs, root sectioning was conducted using an ultrasonic tip with the guides. Mean and standard deviation were calculated for the remaining root length, width, and volume. Data were analyzed using the Kruskal-Wallis test and Dunn's post hoc test. RESULTS: Statistical analysis showed significant differences in root width between the control group (2.0 mm) and both Groups A (2.655 mm; p < 0.0001) and B (2.460 mm; p < 0.0001). Group B (5.585 mm) also showed a significant difference in root remnant compared with the control (13.880 mm; p < 0.0043). Groups A and B did not differ significantly from each other. CONCLUSIONS: The socket shield technique is safe and effective, this study aimed to add improvements through the introduction of surgical guides, facilitating the root section, which is the critical phase so that the parameters that are requisite for success are achieved in terms of width, length, and volume of the root remnant. Both guided techniques effectively facilitated root sectioning, maintaining satisfactory root thickness and length. CLINICAL SIGNIFICANCE: Sectioning the root is challenging for the correct angulation to remove the palatal portion in the socket shield technique. Therefore, modifying this technique with the use of the presented guides, it is possible to prevent damage to the soft tissues and to plan the surgery with the help of cone beam computed tomography (CBCT) scans to remove the root apex and maintain the root remnant with a thickness greater than 1.5 mm, making the socket-shield technique more predictable.

Alargamiento estético de corona con planeación digital mediante el uso de guía quirúrgica / Esthetic crown lengthening with digital planning using a surgical guide

Introducción: el alargamiento de corona relacionado con la estética tiene como objetivo revelar una longitud adecuada de la corona y disminuir la exposición gingival. El procedimiento incluye gingivectomía y alveolectomía para restablecer el tejido gingival supracrestal requerido protésicamente según la dimensión fisiológica. Mediante un escaneo intraoral del maxilar, mandíbula y posición intercuspídea máxima y utilizando un software especializado, se diseña de manera digital la forma de los dientes y el contorno gingival. Este diseño genera una imagen de la restauración estética deseada para el prostodoncista y periodoncista. La fabricación física del diseño es asistida por computadora (CAD-CAM), creando una guía quirúrgica de resina acrílica moldeada al vacío para determinar la longitud de la corona clínica requerida en la cirugía. Objetivo: el caso interdisciplinario presentado describe una técnica innovadora empleando el flujo digital mediante un software que, a partir de un escaneo oral, diseña de manera digital un Mock-Up utilizado como guía para la cirugía periodontal. Presentación del caso: acude a clínica de la Maestría en Periodoncia de la Universidad Autónoma de Coahuila (UAdeC) paciente de 52 años, ASA I, para una cirugía periodontal con fines estéticos. Se procedió a la cirugía, colocando el Mock-Up en la región anterosuperior como guía para la gingivectomía. Luego, se realizó el levantamiento de colgajo antes de la alveolectomía, tomando en cuenta la longitud del tejido gingival supracrestal. Por último, se suturaron los tejidos blandos con técnica de colchonero horizontal. Resultados: siete días después, se retiran los puntos apreciando una cicatrización correcta y uniforme de los tejidos. Conclusiones: este abordaje digital ofrece una reducción significativa del tiempo quirúrgico, además de una estética satisfactoria y una precisa arquitectura gingival (AU)

Introduction: crown lengthening for aesthetic purposes aims to reveal an adequate crown length and reduce gingival exposure. The procedure includes gingivectomy and alveolectomy to restore the prosthetically required supracrestal gingival tissue according to physiological dimensions. Through an intraoral scan of the maxilla, mandible, and maximum intercuspidal position, and using specialized software, the shape of the teeth and the gingival contour are digitally designed. This design generates an image of the desired aesthetic restoration for the prosthodontist and periodontist. The physical fabrication of the design is computer-assisted (CAD-CAM), creating a vacuum-formed acrylic resin surgical guide to determine the clinical crown length required in surgery. Objective: the presented interdisciplinary case describes an innovative technique using digital workflow through software that, from an oral scan, digitally designs a Mock-Up used as a guide for periodontal surgery. Case presentation: a 52-year-old ASA I patient attended the Periodontics Master's clinic at Universidad Autónoma de Coahuila (UAdeC) for periodontal surgery with aesthetic purposes. The surgery was performed by placing the Mock-Up in the anterosuperior region as a guide for gingivectomy. Then, a flap was raised before the alveolectomy, considering the length of the supracrestal gingival tissue. Finally, the soft tissues were sutured with a horizontal mattress suture technique. Results: seven days later, the sutures were removed, showing correct and uniform tissue healing. Conclusions: this digital approach offers a significant reduction in surgical time, in addition to satisfactory esthetics and precise gingival architecture (AU)

Eating behavior and dietary practices are associated with age, sex, body mass index, and body fat in individuals with type 2 diabetes and/or hypertension in Brazil.

Type 2 diabetes (T2DM), hypertension, and obesity are major contributors to global mortality. This study aimed to evaluate eating behavior and dietary practices among individuals with T2DM and/or hypertension, examining their associations with sociodemographic and anthropometric characteristics. We hypothesized that these factors may contribute to body mass index (BMI) and body fat (BF) levels in these individuals. In a cross-sectional study, adults/older individuals diagnosed with T2DM and/or hypertension were included. Eating behavior was evaluated via Three-factor Eating Questionnaire-R21. Dietary practices were assessed using the Dietary Guidelines for the Brazilian Population (DGBP) questionnaire. Poisson regression examined associations between eating behaviors, dietary practices, and sociodemographic factors. Linear regressions were employed to analyze relationships between eating behavior, dietary practices, BMI, and BF. The study included 275 primarily female (70.5%) participants under 60 years old (54.2%). Male and older participants had a lower prevalence of high scores in the 'emotional eating' (EE; PR: 0.40 [0.25;0.63]) and 'uncontrolled eating' (UE; PR: 0.68 [0.50;0.92]) domains. In contrast, older patients had a higher prevalence of adherence to the DGBP recommendations (PR:1.53 [1.20;1.94]). A positive association was observed between high EE (ß:3.71 [1.98;5.44]) and UE (ß:2.85 [1.15;4.55]) scores and BMI, whereas higher dietary practice scores (ß:-2.19 [-3.88;-0.50]) were negatively associated with BMI. High EE (ß:2.20 [0.38;4.02]) and UE (ß:1.92 [0.17;3.67]) scores were positively associated with BF regardless of the confounding factors included. Higher scores on the dietary practices were inversely associated with BF (ß:-1.94 [-3.67;-0.21]). Understanding dietary behaviors and practices can facilitate a more comprehensive and effective treatment approach.

Guía de práctica clínica para la atención a niños y adolescentes con enfermedad renal crónica / Clinical practice guide for the care to children and adolescents with chronic renal disease

La enfermedad renal crónica constituye un problema de salud por su impacto sobre los individuos, la sociedad y la economía. Teniendo en cuenta lo anterior, y luego de una amplia búsqueda bibliográfica, se diseñó una guía de práctica clínica en el Policlínico Luis Augusto Turcios Lima de la provincia de Pinar del Río, dirigida a los profesionales de la atención primaria de salud, con el objetivo de mejorar la calidad de vida de niños y adolescentes con dicha enfermedad. Esta fue elaborada por los métodos de la medicina basada en la evidencia, según el consenso y la opinión de los expertos. Se logró generalizar esta herramienta, emitir recomendaciones y actualizarla acorde con las nuevas evidencias médicas. Finalmente, resultó evaluada por los expertos como muy recomendada.

Chronic renal disease constitutes a health problem due to its impact on individuals, society and economy. Taking into account the above-mentioned, and after a wide literature search, a clinical practice guide was designed in Luis Augusto Turcios Lima Polyclinic from Pinar del Río province, directed to primary health care professionals, aimed at improving the life quality of children and adolescents with this disease. It was elaborated by medicine methods based on the evidence, according to the consent and opinion of experts. It was possible to generalize this tool, give recommendations and up to date according to the new medical evidences. Finally, it was evaluated by experts as very recommended.

Aspectos clinicoepidemiológicos de adultos con guía anterior de la oclusión dentaria disfuncional

Introducción: Los dientes anteriores tienen una función predominante en el sistema estomatognático, esencial para la estética, la fonación y la masticación. Objetivo: Caracterizar a los adultos con guía anterior de la oclusión dentaria disfuncional según variables clínicas y epidemiológicas. Métodos: Se realizó un estudio descriptivo y transversal, desde octubre de 2021 hasta abril de 2022, en la Clínica Estomatológica Docente 3 de Octubre de Las Tunas. El universo estuvo conformado por 825 historias clínicas que contenían el diagnóstico de pacientes con bruxismo, oclusión traumática y trastornos temporomandibulares, quienes presentaban disfunción de la guía anterior de la oclusión dentaria. Mediante el muestreo no probabilístico intencional, fue seleccionada una muestra de 615 con fórmula dentaria íntegra o desdentamiento parcial de clase III de Kennedy y atención estomatológica concluida o inactivación de los procesos de caries e inflamatorios agudos. Resultados: Primaron el sexo femenino (54,8 %) y las edades de 40-49 años (33,5 %). El bruxismo prevaleció como diagnóstico clínico (41,9 %) con predominio del correspondiente al sueño (39,1 %), el secundario (51,2 %), el probable (59,7 %) y el moderado (69,4 %); entre sus clasificaciones más relevantes se destacó el sistema estomatognático disfuncional (84,4 %). Las manifestaciones clínicas dentarias predominantes fueron las facetas de desgaste (87,5 %). Conclusiones: La caracterización de los pacientes con guía anterior de la oclusión dentaria disfuncional permite establecer una panorámica actualizada de esta problemática de salud para un mejor seguimiento y tratamiento a dichos pacientes.

Introduction: Anterior teeth have a predominant function in the stomatognatic system, essential for the aesthetics, phonation and mastication. Objective: To characterize adults with anterior guide of dysfunctional dental occlusion according to clinical and epidemiological variables. Methods: A descriptive and cross-sectional study, was carried out from October, 2021 to April, 2022, in the 3 de Octubre Teaching Stomatological Clinic from Las Tunas. The universe was formed by 825 medical records that contained the diagnosis of patients with bruxism, traumatic occlusion and temporomandibular disorders who presented anterior guide of the dysfunctional dental occlusion. By means of the intentional non probabilistic sampling, a sample of 615 with entire dental formula or class III partial toothlessness of Kennedy and concluded stomatologic care or inactivation of the cavity and acute inflammatory processes was selected. Results: There was a prevalence of the female sex (54.8%) and the 40-49 age group (33.5%). Bruxism prevailed as clinical diagnosis (41.9%) with prevalence of the corresponding to sleep (39.1%), secondary (51.2%), probable (59.7%) and moderate (69.4%); among the most outstanding classifications was the dysfunctional stomatognatic system (84.4%). The predominant dental clinical manifestations were the wear facets (87.5%). Conclusions: The characterization of patients with anterior guide of the dysfunctional dental occlusion allows to establish an up-to-date panoramic of this health problem, for a better follow-up and treatment to these patients.

Guide catheter extension use are associated with higher procedural success in chronic total occlusion percutaneous coronary interventions.

BACKGROUND: Guide catheter extensions (GCEs) increase support and facilitate equipment delivery, but aggressive instrumentation may be associated with a higher risk of complications. AIM: Our aim was to assess the impact of GCEs on procedural success and complications in patients submitted to chronic total occlusion (CTO) percutaneous coronary intervention (PCI). METHODS: We analyzed data from the multicenter LATAM CTO Registry. Procedural success was defined as <30% residual stenosis and TIMI 3 distal flow. Major adverse cardiac and cerebrovascular events (MACCE) was defined as the composite of all-cause death, myocardial infarction, target vessel revascularization, and stroke. Propensity score matching (PSM) was used to compare outcomes with and without GCE use. RESULTS: From August 2010 to August 2021, 3049 patients were included. GCEs were used in 438 patients (14.5%). In unadjusted analysis, patients in the GCE group were older and had more comorbidities. The median J-CTO score and its components were higher in the GCE group. After PSM, procedural success was higher with GCE use (87.7% vs. 80.5%, p = 0.007). The incidence of coronary perforation (odds ratio [OR]: 1.46, 95% confidence interval [CI]: 0.78-2.71, p = 0.230), bleeding (OR: 1.99, 95% CI: 0.41-2.41, p = 0.986), in-hospital death (OR: 1.39, 95% CI: 0.54-3.62, p = 0.495) and MACCE (OR: 1.07, 95% CI: 0.52-2.19, p = 0.850) were similar in both groups. CONCLUSION: In a contemporary, multicenter cohort of patients undergoing CTO PCI, GCEs were used in older patients, with more comorbidities and complex anatomy. After PSM, GCE use was associated with higher procedural success, and similar incidence of adverse outcomes.

High-throughput analysis of the Trypanosoma cruzi minicirculome (mcDNA) unveils structural variation and functional diversity.

Trypanosoma cruzi causes Chagas disease and has a unique extranuclear genome enclosed in a structure called the kinetoplast, which contains circular genomes known as maxi- and minicircles. While the structure and function of maxicircles are well-understood, many aspects of minicircles remain to be discovered. Here, we performed a high-throughput analysis of the minicirculome (mcDNA) in 50 clones isolated from Colombia's diverse T. cruzi I populations. Results indicate that mcDNA comprises four diverse subpopulations with different structures, lengths, and numbers of interspersed semi-conserved (previously termed ultra-conserved regions mHCV) and hypervariable (mHVPs) regions. Analysis of mcDNA ancestry and inter-clone differentiation indicates the interbreeding of minicircle sequence classes is placed along diverse strains and hosts. These results support evidence of the multiclonal dynamics and random bi-parental segregation. Finally, we disclosed the guide RNA repertoire encoded by mcDNA at a clonal scale, and several attributes of its abundance and function are discussed.

Treatment of childhood obesity based on the reduction of ultra-processed foods plus energy restriction: A randomised controlled trial based on the Brazilian guidelines.

Reducing ultra-processed foods (UPF) improves diet quality and may curb energy consumption. This study aimed to compare an intervention based on the reduction of UPF, according to the Dietary Guideline for the Brazilian Population (DGBP), with and without advice on energy intake. A parallel and randomised controlled trial was carried out with children with obesity from 7 to 12 years old. Both control (CG) and intervention groups (IG) participated monthly in 6 standardised educational activities based on the 10 steps of the DGBP. An individualised food plan was also provided to the IG. The rate of change for body mass index (BMI), waist circumference, body weight, and UPF consumption were investigated based on mixed-effect models. At the end of the study, the BMI declined in the IG (Δ = -0.27 kg/m2) compared to the CG (Δ = + 0.53 kg/m2) (p = .0002). Both groups showed a decline in grams of UPF until the fourth month and a gradual increase in the following months. Combining the qualitative approach of the DGBP with counselling on energy restriction through the diet plan proved to be effective in reducing childhood obesity. Clinical Trial Registration: This trial is registered at the Brazilian Registry of Clinical Trials (REBEC), under the RBR-3st5sn registry, available at http://www.ensaiosclinicos.gov.br/rg/RBR-3st5sn/. The datasets generated by the current study are not publicly available but are available from the corresponding author on reasonable request.

Exploring Culture Media Diversity to Produce Fungal Secondary Metabolites and Cyborg Cells.

Fungi are microorganisms of significant biotechnological importance due to their ability to provide food and produce several value-added secondary metabolites and enzymes. Its products move billions of dollars in the pharmaceutical, cosmetics, and additives sectors. These microorganisms also play a notable role in bionanotechnology, leading to the production of hybrid biological-inorganic materials (such as cyborg cells) and the use of their enzyme complex in the biosynthesis of nanoparticles. In this sense, optimizing the fungal growth process is necessary, with selecting the cultivation medium as one of the essential factors for the microorganism to reach its maximum metabolic expression. The culture medium's composition can also impact the nanomaterial's stability and prevent the incorporation of nanoparticles into fungal cells. Therefore, our main objectives are the following: (1) compile and discuss the most commonly employed culture media for the production of fungal secondary metabolites and the formation of cyborg cells, accompanied by preparation methods; (2) provide a six-step guide to investigating the fungal metabolomic profile and (3) discuss the main procedures of microbial cultivation to produce fungal cyborg cells.

Comprehensive Development of a Cellulose Acetate and Soy Protein-Based Scaffold for Nerve Regeneration.

BACKGROUND: The elaboration of biocompatible nerve guide conduits (NGCs) has been studied in recent years as a treatment for total nerve rupture lesions (axonotmesis). Different natural polymers have been used in these studies, including cellulose associated with soy protein. The purpose of this report was to describe manufacturing NGCs suitable for nerve regeneration using the method of dip coating and evaporation of solvent with cellulose acetate (CA) functionalized with soy protein acid hydrolysate (SPAH). METHODS: The manufacturing method and bacterial control precautions for the CA/SPAH NGCs were described. The structure of the NGCs was analyzed under a scanning electron microscope (SEM); porosity was analyzed with a degassing method using a porosimeter. Schwann cell (SCL 4.1/F7) biocompatibility of cell-seeded nerve guide conduits was evaluated with the MTT assay. RESULTS: The method employed allowed an easy elaboration and customization of NGCs, free of bacteria, with pores in the internal surface, and the uniform wall thickness allowed manipulation, which showed flexibility; additionally, the sample was suturable. The NGCs showed initial biocompatibility with Schwann cells, revealing cells adhered to the NGC structure after 5 days. CONCLUSIONS: The fabricated CA/SPAH NGCs showed adequate features to be used for peripheral nerve regeneration studies. Future reports are necessary to discuss the ideal concentration of CA and SPAH and the mechanical and physicochemical properties of this biomaterial.

Incisões e retalhos: orientações e guia prático / Incisions and flaps: guidelines and pratical guide

Na cirurgia oral, dois princípios básicos são o acesso adequado e uma boa visualização. Em diversos casos, isto somente é alcançado afastando os tecidos moles adjacentes do local operado através de retalhos cirúrgicos. Os principais retalhos descritos na literatura para cirurgia oral são: envelope, em L ou triangular, trapezoidal e em Y. As técnicas para a realização de incisões e retalhos são essenciais para o acesso de determinados dentes ou patologias que surgem de maneira rotineira na clínica odontológica, além de também serem usadas para contornar complicações cirúrgicas, como fraturas radiculares. O objetivo deste trabalho é, através de um guia prático, expor os princípios para uma técnica adequada e a sequência para realização dos retalhos mais utilizados em cirurgia oral.

In oral surgery, two basic principles are access and good visualization. In many cases, this is only achieved by moving adjacent soft tissues away from the operated site using surgical flaps. The main flaps described in the literature for oral surgery are: envelope, L-shaped or triangular, trapezoidal and Y-shaped. Techniques for performing incisions and flaps are essential for accessing certain teeth or pathologies that arise routinely in the dental clinic, in addition to being used to circumvent surgical complications, such as root fractures. The objective of this work is, as a practical guide, to expose the principles for an adequate technique and a sequence for the realization of the most used flaps in oral surgery.

O guia brasileiro de atividade física: análise a partir da concepção ampliada de saúde / The brazilian guidelines to physical activity: analysis from the expanded conception of health / La guía brasileña de actividad física: análisis desde la concepción ampliada de la salud

Trata-se de uma pesquisa de caráter documental, com abordagem descritiva e exploratória, com o objetivo de analisar o conteúdo do Guia de Atividade Física para a População Brasileira (GAFPB) a partir da concepção ampliada de saúde. As categorias identificadas foram: Bem-estar e qualidade de vida; Socialização e fortalecimento de vínculos; Autonomia e empoderamento; Participação Social; Ampliação do acesso e complexidades envolvidas. Já as ausências foram: o conceito de saúde no qual o GAFPB foi baseado; associação a um profissional específico; questões relacionadas a populações vulneráveis. Em conclusão, foi possível identificar a presença da concepção ampliada de saúde no GAFPB ao observar o questionamento do privilégio da dimensão biológica e do caráter impositivo e normativo de intervir, se aproximar do debate sobre o desenvolvimento humano e pela relativização do enfoque na quantidade de atividades físicas que deve ser realizada.

This is documentary research with a descriptive and exploratory approach, the objective was to analyze in a categorical-thematic way the content of the Physical Activity Guidelines for the Brazilian Population (GAFPB) based on the expanded conception of health. The categories identified were: Well-being and quality of life; Socialization and strengthening of bonds; Autonomy and empowerment; Social Participation; Expansion of access and complexities involved. The absences were the presentation of the concept of health on which the GAFPB was based; association with a specific professional; issues related to vulnerable populations. In conclusion, it was possible to identify the presence of the expanded conception of health in the GAFPB by questioning the privilege of the biological dimension and the imposing and normative character of intervening, approaching the debate on human development and relativizing the focus on the amount of physical activities that must be performed.

Se trata de una investigación documental con abordaje descriptivo y exploratorio, el objetivo fue analizar de forma categórica-temática el contenido de la Guía de Actividad Física para la Población Brasileña (GAFPB) a partir de la concepción ampliada de la salud. Las categorías identificadas fueron: Bienestar y calidad de vida; Socialización y fortalecimiento de vínculos; Autonomía y empoderamiento; Participación social; Ampliación de acceso y complejidades involucradas. Las ausencias fueron la presentación del concepto de salud en el que se basó la GAFPB; vinculación con un profesional específico; temas relacionados con las poblaciones vulnerables. En conclusión, fue posible identificar la presencia de la concepción ampliada de la salud en el GAFPB al cuestionar el privilegio de la dimensión biológica y el carácter imponente y normativo de intervenir, acercándose al debate sobre el desarrollo humano y relativizando el enfoque en la cantidad de actividades físicas deben ser realizadas.

A guide for systematic reviews of observational studies / Guía para revisiones sistemáticas de estudios observacionales / Guia para revisão sistematica de estudos observacionais

ABSTRACT Objective: to describe a guide for the development of systematic reviews of observational studies and systematization of international guidelines and tools, focusing on diverse evidence for innovation and clinical practice. Method: this theoretical-conceptual study was initiated during the development of a systematic review with meta-analysis of observational studies, using international guidelines and tools. Results: a guide was constructed to develop systematic reviews of observational studies. Diverse information about several stages and requirements for conducting a systematic review based on international guidelines and tools was systematized, aiming to ensure scientific rigor in manuscripts written by professionals from the health area. Conclusion: this study contributes to research in the health area by innovatively synthesizing guidance on the systematic review method and approaches. The references herein used serve as a starting point for understanding the procedures and international tools necessary for a systematic review of observational studies.

RESUMEN Objetivo: describir una guía para desarrollar revisiones sistemáticas de estudios observacionales y sistematizar pautas y herramientas internacionales, concentrándose en evidencias para la innovación y la práctica clínica. Método: este estudio teórico-conceptual se inició mientras se desarrollaba una revisión sistemática con metaanálisis de estudios observacionales en la que se utilizaron directrices y herramientas internacionales. Resultados: se elaboró una guía para desarrollar revisiones sistemáticas de estudios observacionales. Se sistematizó diversa información sobre varias etapas y requisitos para realizar una revisión sistemática basada en directrices y herramientas internacionales, con el objetivo de garantizar el rigor científico en los manuscritos redactados por profesionales del área de salud. Conclusión: este estudio representa un innovador aporte a la investigación en el área de salud porque sintetiza las pautas sobre el método y los enfoques de las revisiones sistemáticas. Las referencias utilizadas en este trabajo son un buen punto de partida para comprender los procedimientos y las herramientas internacionales necesarios para una revisión sistemática de estudios observacionales.

RESUMO Objetivo: descrever um guia para o desenvolvimento de revisões sistemáticas de estudos observacionais, sistematização de orientações e ferramentas internacionais, com foco em evidências para inovação e prática clínica. Método: este estudo teórico-conceitual foi iniciado durante o desenvolvimento de uma revisão sistemática com metanálise de estudos observacionais em que foram usadas diretrizes e ferramentas internacionais. Resultados: foi construído um guia para desenvolver revisões sistemáticas de estudos observacionais. Foram sistematizadas informações sobre várias etapas e requisitos para realizar revisão sistemática pautada em diretrizes e ferramentas internacionais, visando rigor científico nos manuscritos redigidos por profissionais da área da saúde. Conclusão: este estudo é uma contribuição à pesquisa na área da saúde que inova ao sintetizar a orientação sobre o método e abordagens de revisão sistemática. As referências aqui usadas são um ponto de partida para compreender os procedimentos e ferramentas internacionais necessários a uma revisão sistemática de estudos observacionais.

An ex vitro hairy root system from petioles of detached soybean leaves for in planta screening of target genes and CRISPR strategies associated with nematode bioassays.

MAIN CONCLUSION: The ex vitro hairy root system from petioles of detached soybean leaves allows the functional validation of genes using classical transgenesis and CRISPR strategies (e.g., sgRNA validation, gene activation) associated with nematode bioassays. Agrobacterium rhizogenes-mediated root transformation has been widely used in soybean for the functional validation of target genes in classical transgenesis and single-guide RNA (sgRNA) in CRISPR-based technologies. Initial data showed that in vitro hairy root induction from soybean cotyledons and hypocotyls were not the most suitable strategies for simultaneous performing genetic studies and nematode bioassays. Therefore, an ex vitro hairy root system was developed for in planta screening of target molecules during soybean parasitism by root-knot nematodes (RKNs). Applying this method, hairy roots were successfully induced by A. rhizogenes from petioles of detached soybean leaves. The soybean GmPR10 and GmGST genes were then constitutively overexpressed in both soybean hairy roots and tobacco plants, showing a reduction in the number of Meloidogyne incognita-induced galls of up to 41% and 39%, respectively. In addition, this system was evaluated for upregulation of the endogenous GmExpA and GmExpLB genes by CRISPR/dCas9, showing high levels of gene activation and reductions in gall number of up to 58.7% and 67.4%, respectively. Furthermore, morphological and histological analyses of the galls were successfully performed. These collective data validate the ex vitro hairy root system for screening target genes, using classical overexpression and CRISPR approaches, directly in soybean in a simple manner and associated with nematode bioassays. This system can also be used in other root pathosystems for analyses of gene function and studies of parasite interactions with plants, as well as for other purposes such as studies of root biology and promoter characterization.

Confecção de guia multifuncional para planejamento de reabilitação implantossuportada: relato de caso / Preparation of a multifuncional guide for the planning of implant-supported rehabilitations: case report / Elaboración de una guía multifuncional para la planificación de rehabilitación implantosoportada: informede caso

Introdução:Em busca da estética e da função mastigatória,é cada vez mais crescente aprocurapor reabilitações implantossuportadas. O guia multifuncional surgepara orientar a disponibilidade óssea e contribuir no planejamento da instalação tridimensional dos implantes, seguindo os princípios do planejamento reverso.Objetivo:Descrever, por meio de um caso clínico, a possibilidade de obtenção de resultados de excelente previsibilidade em coroas unitárias implantossuportadas, por meio do uso de guias multifuncionais, no planejamento do início ao fim de tratamento.Relato decaso:Paciente A.M.F, 44 anos, sexo feminino, compareceu à clínica de Prótese dentária do Departamento de Odontologia/UFRN com queixa de insatisfação da sua prótese removível e harmonia do sorriso. Ao exame clínico e radiográfico, observou-se ausência do elemento 12, apresentando um espaço interoclusal de 5mm e distância médio-distal de 7mm. Foi confeccionado o guia em resina acrílica, o qual foi preenchido o espaço desdentado com um dente de estoque. Em seguida, o dente deste guia teve seu centro perfurado com uma broca esférica para peça reta na região de cíngulo e com isso, preenchida com guta percha em bastão. Após isso, o paciente foi encaminhado para realizar uma tomografia computadorizada cone beam com o guia multifuncional em posição.As imagens obtidas permitiram o planejamento para instalação do implante, como inclinação e posicionamento favorável, bem como ausência de disponibilidade óssea na região. Além disso, o guia funcionou em outras etapas do tratamento da paciente, como na fase provisória.Conclusão:Os guias auxiliam em diversas fases do tratamento e permitem maior previsibilidade dos resultados em reabilitações protéticas unitárias implantossuportadas, apresentando-se como um dispositivo promissorpara ocorreto posicionamento do implante (AU).

Introduction:In search of esthetics and improved masticatory function, the demand for implant-supported rehabilitation is increasing. Multifunctional guides emerge to assess bone availability and help plan the three-dimensional installation of implants, following the principles of reverse planning. Objective:To describe, through a clinical case, the possibility of obtaining excellent predictability in implant-supported single crowns, through the use of multifunctional guides, inthe planning of a treatment from beginning to end. Case report:Patient A.M.F, 44 years old, female, came to the Prosthodontics clinic at the Department of Dentistry/UFRN complaining of dissatisfaction with her removable prosthesis and the harmony of her smile. Clinical and radiographic examination revealed the absence of element 12, with an interocclusal space of 5mm and a mid-distal distance of 7mm. The acrylic resin guide was made and the edentulous space was filled with a stock tooth. The center of theguide tooth was then drilled with a spherical straight-bit burr in the cingulum region and filled with gutta-percha stick. The patient was then referred for a cone beam computed tomography with the multifunctional guide in position. The images obtained allowed planning for implant installation, such as favorable inclination and positioning, as well as the absence of bone availability in the region. In addition, the guide was effective during other stages of the patient's treatment, such as the provisional phase.Conclusion:The guides assist in various phases of treatment and allow greater predictability of results in implant-supported single prosthetic rehabilitations, presenting themselves as a promising device for correct implant positioning (AU).

Introducción:En busca de estética y función masticatoria, la demanda de rehabilitaciones implantosoportadas es cada vez mayor. La guía multifuncional hasurgido para orientar la disponibilidad ósea y ayudar a planificar la instalación tridimensional de implantes, siguiendo los principios de la planificación inversa. Objetivo: Describir, a través de un caso clínico, la posibilidad de obtener una excelente predictibilidad en coronas unitarias implantosoportadas, mediante el uso de guías multifuncionales, en la planificación desde el início hasta el final del tratamiento. Informe de caso: Paciente A.M.F, 44 años, sexo femenino, compareció a la clínica de Prostodoncia del Departamento de Odontología/UFRN quejándose estar insatisfecha con su prótesis removible y con la armonía de su sonrisa. El examen clínico y radiográfico reveló la ausencia del elemento 12, con un espacio interoclusal de 5 mm y una distancia medio-distal de 7 mm. Se confeccionó una guía de resina acrílica y se rellenó el espacio edéntulo con un diente provisorio. A continuación, se perforó el centro del diente guía con una broca recta esférica en la región del cíngulo y se le rellenó con gutapercha en barra. Posteriormente, el paciente fue remitido a una tomografía computarizada cone beamcon la guía multifuncional en posición. Las imágenes obtenidas permitieron planificar la instalación del implante, como inclinación y posicionamiento favorables, así como la ausencia de disponibilidad ósea en la región. La guía también funcionó en otras fases del tratamiento del paciente, como en la fase provisional. Conclusión:Las guías ayudan en varias fases del tratamiento y permiten una mayor previsibilidadde los resultados en rehabilitaciones protésicas unitarias implantosoportadas, presentándose como un dispositivo prometedor para el correcto posicionamiento de los implantes (AU).

Incidencia de eventos adversos prevenibles en servicios de hospitalización de una clínica de la ciudad de Sincelejo (Colombia) / Incidence of preventable adverse events in the hospitalization services of a Clinic from Sincelejo city (Colombia)

RESUMEN En la actualidad, un parámetro importante de satisfacción del cliente en las IPS es la seguridad. Anualmente se reportan 134 millones de eventos adversos (EA) debido a la atención insegura, lo que ocasiona un gran número de muertes y aumento en los costos de la atención. Objetivo: Estimar la incidencia de eventos adversos prevenibles en los servicios de hospitalización de una clínica de la ciudad de Sincelejo (Colombia) en 2021. Métodos: Estudio de incidencia en 360 historias clínicas de pacientes ingresados en los servicios de hospitalización durante los meses de enero a junio de 2021 en una clínica de la ciudad de Sincelejo, Colombia. Se aplicó la Guía de Cribado para búsqueda de indicios de Eventos Adversos (EA); a los pacientes con cribado positivo se les aplicó el módulo A de identificación de EA, contenido en el formulario modular para revisión retrospectiva de casos. Se realizó análisis descriptivo y evaluación de asociaciones. Resultados: Se obtuvieron 54 indicios de EA, la incidencia fue de 6,11 % de EA prevenibles, la mayoría de estos moderados 54,5 % y severos 31,8 %. La especialidad con mayor incidencia fue Medicina Interna (45,5 %), seguido de las especialidades quirúrgicas 36,4 %; Cabe resaltar que el 54,5 % de los eventos estuvo relacionado con factores de riesgo extrínsecos. Conclusiones: Este es el primer estudio realizado en la institución y permitió identificar la incidencia de eventos adversos prevenibles a través de la Guía de Cribado para la revisión retrospectiva de las historias clínicas, que mostró ser una herramienta útil para este fin.

ABSTRACT Currently, an important parameter of customer satisfaction in HPI is health safety. It is reported annually 134 million of adverse events (AE) due to unsafe attention, which causes large numbers of deaths and increased costs of care. Objective: The research estimates the incidence of preventable adverse events in the hospitalization services of a Clinic from Sincelejo city in 2021. Methods: Study of incidence in 360 clinical records of patients admitted to the hospitalization service from January to June of 2021 in a Clinic from Sincelejo city, Colombia. The Screening Guide was applied to search for signs of adverse events (AE); Module A for the identification of AE, contained in the modular form for retrospective review of cases, was applied to patients with positive Screening Guide. Descriptive analysis and evaluation of associations were performed. Results: 54 signs of AE were obtained, the incidence was of 6.11 % of preventable AE, most of these moderate 54.5 % and severe 31.8 %. The specialty with most incidence was Internal Medicine (45.5 %), followed by surgical specialties 36.4 %; It should be noted that 54.5 % of the events were related to extrinsic risk factors. Conclusions: This is the first study carried out in the institution and it allowed us to identify the incidence of preventable adverse events through the Screening Guide for the retrospective review of medical records, which proved to be a useful profiling tool for this purpose.

A comprehensive guide to BCI-based stroke neurorehabilitation interventions.

Brain-Computer Interfaces (BCIs) offer the potential to facilitate neurorehabilitation in stroke patients by decoding user intentions from the central nervous system, thereby enabling control over external devices. Despite their promise, the diverse range of intervention parameters and technical challenges in clinical settings have hindered the accumulation of substantial evidence supporting the efficacy and effectiveness of BCIs in stroke rehabilitation. This article introduces a practical guide designed to navigate through these challenges in conducting BCI interventions for stroke rehabilitation. Applicable regardless of infrastructure and study design limitations, this guide acts as a comprehensive reference for executing BCI-based stroke interventions. Furthermore, it encapsulates insights gleaned from administering hundreds of BCI rehabilitation sessions to stroke patients.•Presents a comprehensive methodology for implementing BCI-based upper extremity therapy in stroke patients.•Provides detailed guidance on the number of sessions, trials, as well as the necessary hardware and software for effective intervention.

Factors Associated with Adherence to the Brazilian Food Guide in Food Service Workers.

The present study aimed to evaluate factors associated with adherence to the Food Guide for the Brazilian Population (GAPB) among food service workers. A cross-sectional study was conducted with 421 employees from 43 food service establishments located in a capital of Brazil. Health and lifestyle data were collected, including the continuous use of medication, smoking, physical activity, and alcohol consumption. An instrument based on the GAPB was utilized, covering the domains of planning, household organization, eating habits, and food choices, according to GAPB recommendations. The findings revealed a direct association between the quality of life scores and the total GAPB adherence score (ß = 1.17; p < 0.001), as well as the domains of planning (ß = 0.53; p < 0.001), household organization (ß = 0.22; p = 0.001), and eating habits (ß = 0.38; p < 0.001). Adherence to the GAPB among food service workers was strongly linked to their perception of quality of life, in addition to other factors such as gender, age, education, type of work activity, and variables of lifestyle. Therefore, the eating practices of these employees need to be assessed comprehensively, and enhancing their quality of life can encourage proper and healthy eating.