RESUMO
BACKGROUND: This study aimed at exploring the effect of microRNA-129-5p (miR-129-5p) targeting high mobility group box-1 (HMGB1)-receptor for advanced glycation end-products (RAGE) signaling pathway on the revascularization in a collagenase-induced intracerebral hemorrhage (ICH) rat model. METHODS: OX26-pGFAP-IL, an immunoliposome expressing miR-129-5p was constructed. The collagenase-induced ICH rat models were successfully established by 96 Sprague Dawley (SD) rats, which were categorized into the sham group, ICH group, miR-129-5p group, negative control (NC) group, ethyl pyruvate (EP, an inhibitor of HMGB1) group and N-benzyl-4-chloro-N-cyclohe-xylbenzamide (FPS-ZM1, a RAGE receptor antagonist) group. The miR-129-5p expression in the brain tissue homogenate was detected using the quantitative real-time polymerase chain reaction (qRT-PCR) and the protein expressions of HMGB1 and RAGE by Western blotting. Immunohistochemistry (IHC) was used for the detection of the vascular endothelial growth factor (VEGF). Microvessel density (MVD) was also detected. RESULTS: Compared to the sham group, the ICH, NC, EP and FPS-ZM1 groups had a decrease in miR-129-5p expressions, and an increase in the protein expressions of HMGB1, RAGE and VEGF and MVD. In comparison to the ICH, NC, EP and FPS-ZM1 groups the miR-129-5p group had an elevation in the miRNA-129-5p expressions. The miR-129-5p and EP groups had decreased HMGB1 protein expression and the miR-129-5p, EP and FPS-ZM1 groups had a reduced RAGE protein expression as compared to the ICH group. In comparison to the ICH group, the miR-129-5p, EP, FPS-ZM1 groups had a decline in the VEGF protein expression and MVD. CONCLUSION: Our study proved that up-regulation of miR-129-5p might suppress the HMGB1-RAGE signaling pathway to restrain the revascularization of rats with ICH.
Assuntos
Hemorragia Cerebral/metabolismo , Proteína HMGB1/metabolismo , MicroRNAs/metabolismo , Receptor para Produtos Finais de Glicação Avançada/metabolismo , Animais , Benzamidas/metabolismo , Encéfalo/metabolismo , Colagenases/metabolismo , Modelos Animais de Doenças , Feminino , Masculino , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/fisiologia , Regulação para Cima/fisiologia , Fator A de Crescimento do Endotélio Vascular/metabolismoRESUMO
Objective To study the value of serum HMGB1(high mobility group box-1) and carbohydrate antigen 72-4(CA72-4) in patients with early gastric cancer .Methods 50 cases of early gastric cancer patients were enrolled as a group ,45 cases of gastric lesions as benign gastric benign group ,100 cases of healthy people as healthy group.Serum HMGB1 and CA72-4 in all people were detected .Results Early gastric cancer serum HMGB1 and CA72-4 was higher than benign lesions in the stomach and the healthy group (χ2 =33.69,82.95,51.41,104.74, P0.05).HMGB1 diagnosis of early gastric cancer sensitivity was 70.0%,specific-ity 95.2%and accuracy was 88.7%.CA72-4 diagnosis of early gastric cancer with a sensitivity of 80.0%(40/50), specificity was 97.2%(141/145) an accuracy of 92.8%(181/195).Joint detection,diagnosis of early gastric cancer with a sensitivity of 94.0%(47/50),while the specificity was 93.1%(135/145),the accuracy was 93.3%(182/195),higher than those of the single detection sensitivity and accuracy .Early gastric cancer with lymph node metasta-sis in patients with serum HMGB1 and CA72-4 were significantly higher than those in patients without lymph node metastasis,the two groups were significantly different (t=2.927,4.096,all P<0.05).Conclusion Joint detection of serum HMGB1 and CA72-4 allows early diagnosis of gastric cancer ,and to determine the prognosis of patients .
