The structure of wing in the earliest Permopsocida.

Permopsocids are small acercarian insects with mouthparts specialized for sucking. They are closely related to Hemiptera and Thysanoptera. The earliest known representatives are from the Early Permian. Here evidence is presented that the Permopsocida occurred even earlier in Pennsylvanian (Moscovian) deposits in the Piesberg quarry near Osnabrück (Lower Saxony, Germany). This material is assigned to the Permian family Psocidiidae; Carbonopsocus mercuryi gen. et sp. nov., based on the wing venation diagnosed by the unique branching pattern of the main veins, the shape of the areola postica being longer than wide, the angular shape of the pterostigma, the ir crossvein directed proximally mid of pterostigma (apomorphy) and the vannus formed by the three veins of PCu, A1 and A2. The shape of the veins, with a Y-vein formed by the distal fusion of PCu with A1, could be a putative symplesiomorphy of the Psocodea with Permopsocida and Hemiptera. C. mercuryi gen. et sp. nov. is the first appearance date for Permopsocida and roots the Acercaria tree. In addition, another specimen of Dichentomum cf. arroyo (Psocidiidae) from Carrizo Arroyo is presented and figured, confirming the presence of the genus Dichentomum near the Carboniferous-Permian boundary and linking it to the Artinskian species from Elmo in Kansas, USA.

We know very little about pollination in the Platanthera Rich (Orchidaceae: Orchidoideae).

The Platanthera Rich. (Orchidoideae) comprise a speciose genus of orchids primarily in the northern hemisphere, with up to 200 known species worldwide. Individual species are known to self-pollinate, but many rely on insect pollinators with characteristics such as floral color, timing of floral odor emissions, nectar rewards, and spur length associated with particular pollination syndromes. As with many orchids, some orchid-pollinator associations are likely highly co-evolved, but we also know that some Platanthera spp. are the result of hybridization events, which implies a lack of pollinator fidelity in some cases. Some Platanthera spp. occur in large numbers which, coupled with the numerous Platanthera-pollinator systems, make them accessible as study species and useful for co-evolutionary studies. Due to the likely effects of climate change and ongoing development on Platanthera spp. habitats, these orchids and their associated pollinators should be a focus of conservation attention and management. However, while there is a fairly substantial literature coverage of Platanthera-pollinator occurrence and interactions, there are still wide gaps in our understanding of the species involved in these systems. In this systematic review, we outline what is current knowledge and provide guidance on further research that will increase our understanding of orchid-insect co-evolutionary relationships. Our review covers 157 orchid species and about 233 pollinator species interacting with 30 Platanthera spp. We provide analyses on aspects of these interactions such as flower morphology, known insect partners of Platanthera species, insect-Platanthera specificity, pollination visitor timing (diurnal vs. nocturnal), floral rewards, and insect behavior affecting pollination outcomes (e.g., pollinia placement). A substantial number of Platanthera spp. and at least a few of their known pollinators are of official (IUCN) conservation concern - and many of their pollinators remain unassessed or even currently unknown - which adds to the urgency of further research on these co-evolved relationships.

Revealing a Novel Potential Pest of Plum Trees in the Caucasus: A Species Resembling the European Leaf-Mining Stigmella plagicolella, Nepticulidae.

In instances of severe infestations, Nepticulidae larvae can inflict damage on cultivated plants. Previously, it was assumed that the Prunus-feeding Nepticulidae have continuous distribution from Europe to the neighboring Caucasus. During recent fieldwork in the Caucasus, leaf mines were found on plum trees that initially resembled those of Stigmella plagicolella (Stainton) in Europe. However, upon rearing the adults, significant differences emerged, leading to the hypothesis that a different Prunus-feeding species exists in the Caucasus; this challenges previous records in Western Asia. This paper presents the outcomes of our morphological, molecular, and statistical investigations, unveiling S. colchica sp. nov., a previously unknown potential plum-tree pest. Distinguished by male genitalia characteristics, the new species differs from S. plagicolella. The inter- and intraspecific divergences between S. colchica sp. nov. and S. plagicolella range from 3.5% to 6.02%. Moreover, the utilized delimitation algorithms reliably clustered two species separately, as does our mitotype network. A statistical analysis also shows a discernible trend between the leaf mines of S. colchica sp. nov. and S. plagicolella. This unexpected discovery not only documents a new potential pest, enhancing our understanding of the Caucasian fauna, but also contributes to the broader biological inventory.

A crane fly of the genus Gynoplistia Macquart (Diptera, Limoniidae) from the early Miocene of New Zealand.

The first fossil limoniid fly from the Miocene Fossil-Lagerstätte of Foulden Maar in New Zealand is described on the basis of an isolated well-preserved wing. The specimen is tentatively attributed to a new species Gynoplistiafouldensensissp. nov. in the large extant genus Gynoplistia, which is well diversified in the country. It is the second fossil record of this genus, the first one being an isolated wing from the Cretaceous Weald Clay Formation in the United Kingdom.

Amazons Are Back: Absence of Males in a Praying Mantis from Uruguayan Savannas.

With a broad distribution throughout South America, Brunneria subaptera (Saussure) (Coptopterygidae) is the most abundant praying mantis species in Uruguay, mostly associated with grassland vegetation. Their body coloration can vary between green or brown sepia. The recent catalog of Uruguayan mantids showed that all specimens of B. subaptera from the collections in Uruguay were females, suggesting an absence of males in natural populations. The aim of this study was to determine the occurrence of female-only populations of B. subaptera in Uruguay. Moreover, we analyzed the genetic variation and estimated the time of the most recent common ancestor of the species. We performed bimonthly samplings in two localities in Uruguay for 1 year and we sexed the individuals. We extracted DNA from the abdominal tissue of adult females and sequenced a fragment of the mitochondrial cox1 gene to assess the genetic diversity and to estimate diversification times. We did not find any males in either of the studied localities. The age estimates revealed a recent origin of the species (2.33 Ma), and there was no genetic differentiation between the individuals from Uruguayan localities. This absence of males of B. subaptera documented in the sampled locations suggests that the species reproduces by thelytokous parthenogenesis. This study opens promising venues for future research into reproductive strategies and polymorphism in South-American praying mantises that inhabit one of the largest areas of grasslands in the world, currently under dramatic deterioration and reduction.

Retinal Adaptation in Response to Light and Dark Regimes in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae).

The oriental armyworm, Mythimna separata (Walker), is a well-known nocturnal migratory pest that relies on its exceptional nocturnal vision for navigation during long-distance flights. In this study, we investigated the ultrastructure of the compound eyes of adult M. separata using transmission electron microscopy and quantitatively evaluated adaptational changes in the retina under light and dark conditions. The compound eyes of M. separata are superposition eyes with a clear zone. The retina shows remarkable anatomical differences under light and dark adaptation, primarily characterized by distinct patterns of rhabdoms within the clear zone: the rhabdoms are nearly absent under light adaptation, but become more voluminous under dark adaptation. In the distal, middle, and proximal sections of the clear zone, the cross-sectional areas of retinulae and rhabdoms, as well as the rhabdom occupation ratio, are significantly larger under dark adaptation than under light adaptation. Conversely, the opposite trend is observed beneath the clear zone. These results indicate remarkable plasticity in the M. separata retina throughout a normal daily cycle, providing a theoretical basis for improving searchlight and ground light trap techniques for the management of this migratory species.

A comparison of dissection and 3D approaches to estimate muscle physiological cross-sectional area, validated by in vivo bite forces.

Performance traits such as bite forces are crucial to fitness and relate to the niche and adaptation of species. However, for many insects it is not possible to directly measure bite forces because they are too small. Biomechanical models of bite forces are therefore relevant to test hypotheses of adaptation in insects and other small organisms. Although such models are based on classical mechanics, combining forces, material properties and laws of levers, it is currently unknown how various models relate to bite forces measured in vivo. One critical component of these models is the physiological cross-sectional area (PCSA) of muscles, which relates to the maximum amount of force they can produce. Here, using the grasshopper Schistocerca gregaria, we compare various ways to obtain PCSA values and use in vivo measurements of bite forces to validate the biomechanical models. We show that most approaches used to derive PCSA (dissection, 3D muscle convex hull volume, muscle attachment area) are consistent with the expected relationships between PCSA and bite force, as well as with the muscle stress values known for insects. The only exception to this are PCSA values estimated by direct 3D muscle volume computation, which could be explained by noisy variation produced by shrinkage. This method therefore produces PCSA values which are uncorrelated to in vivo bite forces. Furthermore, despite the fact that all other methods do not significantly differ from expectations, their derived PCSA values vary widely, suggesting a lack of comparability between studies relying on different methods.

Trichoptera Life Stages Present Distinct Responses to Environmental Conditions in Amazonian Streams.

Biological communities have their biodiversity patterns affected by environmental, spatial, and biogeographic factors that vary from taxa to taxa, and often between life stages. This is especially true when there are differences in the habitat the species use in each of them. Individuals of the insect order Trichoptera are mostly aquatic in their larval stage and terrestrial in their adult stage, which may result in different behaviors and environmental requirements. Our goal was to evaluate the congruence between the larval and adult stages of Trichoptera in Amazonian streams regarding their abundance, richness, and assemblage composition. Additionally, we tried to identify the main environmental factors related to each life stage. For this, larvae and adults of Trichoptera were sampled in the same sites at 12 streams in the Caxiuanã National Forest, Pará state, Brazil. Adult assemblages had greater richness of genera and abundance of individuals than the larval ones, and there was no congruence in the genera composition between these life stages. Our results also showed that different environmental variables structured Trichoptera larvae and adults. Since the sampling of larvae and adults proved to be complementary in the studied streams, we advise that Trichoptera diversity surveys consider both life stages of these organisms.

First Report of Mitochondrial DNA Copy Number Variation in Opsius heydeni (Insecta, Hemiptera, Cicadellidae) from Polluted and Control Sites.

Mitochondrial DNA easily undergoes alterations due to exposure to stress factors. In particular, mitochondrial DNA copy number (mtDNAcn) variation can be used as a biomarker of the effect of exposure to various environmental contaminants. In this study, a molecular investigation based on the evaluation of mtDNAcn variation was applied for the first time to individuals belonging to the species Opsius heydeni. A total of 20 samples were collected from two sites in eastern Sicily: Priolo Gargallo, a site with a strong anthropic impact, and the Simeto river Oasis, a control site. Specimens identified based on morphological traits were used to obtain COI gene sequences from this species that were not previously available in GenBank. After processing, the relative mtDNAcn was evaluated using real-time PCR of a portion of the COI and 18S genes. A decrease in the mtDNAcn in the specimens from the polluted site was observed. These results highlight how environmental contaminants can alter the mitochondrial genome and how Opsius heydeni can be considered a potential bioindicator species of environmental quality.

New insights into immune genes and other expanded gene families of the house fly, Musca domestica, from an improved whole genome sequence.

The house fly, Musca domestica, is a pest of livestock, transmits pathogens of human diseases, and is a model organism in multiple biological research areas. The first house fly genome assembly was published in 2014 and has been of tremendous use to the community of house fly biologists, but that genome is discontiguous and incomplete by contemporary standards. To improve the house fly reference genome, we sequenced, assembled, and annotated the house fly genome using improved techniques and technologies that were not available at the time of the original genome sequencing project. The new genome assembly is substantially more contiguous and complete than the previous genome. The new genome assembly has a scaffold N50 of 12.46 Mb, which is a 50-fold improvement over the previous assembly. In addition, the new genome assembly is within 1% of the estimated genome size based on flow cytometry, whereas the previous assembly was missing nearly one-third of the predicted genome sequence. The improved genome assembly has much more contiguous scaffolds containing large gene families. To provide an example of the benefit of the new genome, we used it to investigate tandemly arrayed immune gene families. The new contiguous assembly of these loci provides a clearer picture of the regulation of the expression of immune genes, and it leads to new insights into the selection pressures that shape their evolution.

First report of parasitism by Eutrichophilus cercolabes (Phthiraptera: Trichodectidae) on Coendou spinosus (Erethizontidae) in Rio Grande do Sul, Brazil - case report.

Coendou spinosus is a species of rodent popularly known as porcupine, it has a great ability to adapt to different habitats and is found in tropical forests in countries such as Bolivia, Brazil, Venezuela and Guianas. This mammal has already been identified as a reservoir of several pathogenic agents for humans and other animals and has a variety of ectoparasites, endoparasites and hemoparasites little studied and described. Due to this, the objective was to report the parasitism by Eutrichophilus cercolabes in C. spinosus in the central region of Rio Grande do Sul, southern Brazil. In total, 16 lice were found, one male and 15 females of the species. This is the first report of the parasitism of this Phthiraptera on C. spinosus in Rio Grande do Sul. The scarcity of reports on the taxonomy and biotic characteristics, as well as the vector capacity of pathogens of most species of ectoparasites of wild animals, highlights the need for further studies on the distribution of these arthropods in different regions and host species.

Coendou spinosus é uma espécie de roedor conhecida popularmente por porco-espinho, possui uma grande capacidade de adaptação a diferentes habitats sendo encontrada em florestas tropicais de países como Bolívia, Brasil, Venezuela e Guianas. Esse mamífero já foi identificado como reservatório de vários agentes patogênicos para o homem e outros animais e possui uma variedade de ectoparasitos, endoparasitos e hemoparasitos pouco estudados e descritos. Devido a isso, objetivou-se relatar o parasitismo por Eutrichophilus cercolabes em C. spinosus na região central do Rio Grande do Sul, Sul do Brasil. Ao total, foram encontrados 16 piolhos, sendo um macho e 15 fêmeas da espécie. Esse é o primeiro relato do parasitismo deste Phthiraptera em C. spinosus no Rio Grande do Sul. A escassez de relatos sobre a taxonomia e características bióticas, bem como a capacidade vetorial de patógenos da maioria das espécies de ectoparasitos de animais silvestres evidencia a necessidade da realização de mais estudos sobre a distribuição desses artrópodes em diferentes regiões e espécies hospedeiras.

Butterfly wing color made of pigmented liquid.

A previously undescribed mechanism underlying butterfly wing coloration patterns was discovered in two distantly related butterfly species, Siproeta stelenes and Philaethria diatonica. These butterflies have bright green wings, but the color pattern is not derived from solid pigments or nanostructures of the scales or from the color of the cuticular membrane but rather from a liquid retained in the wing membrane. Wing structure differs between the green and non-green areas. In the non-green region, the upper and lower cuticular membranes are attached to each other, whereas in the green region, we observed a space of 5-10 µm where green liquid is held and living cells are present. A pigment analysis and tracer experiment revealed that the color of the liquid is derived from hemolymph components, bilin and carotenoid pigments. This discovery broadens our understanding of the diverse ways in which butterfly wings obtain their coloration and patterns.

First record of a rare case of accidental urinary myiasis by Telmatoscopus albipunctata (Diptera: Psychodidae), with morphological description, in a human in Brazil.

Telmatoscopus albipunctata is an insect of the Psychodidae family, with a worldwide distribution, particularly in tropical and subtropical countries. Although it does not have hematophagous habits, it has veterinary medical importance due to the mechanical transmission of protozoa and bacteria, many of which cause nosocomial infections. This dipteran has been reported as one of the causative agents of accidental myiasis in humans in several countries, and since it has not been registered in South America, the objective of the present report was to describe a rare case of accidental urinary myiasis caused by T. albipunctata (Diptera: Psychodidae) in humans in Brazil. In the present report, a 25-year-old female patient, resident of the central region of the state of Rio Grande do Sul, Brazil, sought medical attention after finding larvae in her urine. She complained of vaginal itching and skin dermatitis. The larvae were sent to the Veterinary Parasitology Laboratory of the Federal University of Santa Maria, where they were mounted on permanent slides for further taxonomic identification. Morphological characteristics allowed the identification of fourth-stage larvae and pupae of T. albipunctata. Therefore, the present work reports the first record of accidental urinary myiasis caused by T. albipunctata infection in Brazil and South America.

Insect fauna including unrecorded species in Ulleungdo, South Korea.

Background: Ulleungdo harbours a unique ecosystem owing to its isolation from the mainland alongside its maritime climate. The island, formed via volcanic activity, is the largest island in the East Sea of Korea and retains a primeval forest. The ecosystems are being destroyed owing to increasing human activity on the island. Therefore, through the investigation of the insect fauna of Ulleungdo, we tried to provide information that can be the basis for understanding the island ecology of Ulleungdo. This survey was conducted four times between April and October in 2020 at Seonginbong. New information: The findings of the survey regarding insect fauna at Seonginbong, Ulleungdo included 10 orders, 105 families, 216 genera and 212 species, of which 12 families, two subfamilies, 13 genera and 74 species were previously unrecorded. The data have been registered in the Global Biodiversity Information Facility (GBIF; www.GBIF.org).

Composition and Dynamics of Hexapod Communities on Yushan Bamboo (Yushania niitakayamensis) in the Subtropical Montane Areas of Taiwan.

Plant communities that colonize high-elevation zones generally have short growing seasons which lead to specialized adaptations in such zones. In montane areas of Taiwan, Yushan bamboo (YB, Yushania niitakayamensis) is dominant at elevations ranging from 2500 to 3300 m and grows in a grassland-like open habitat. In this study, hexapods were collected from YB bimonthly between 2009 and 2012 by using a sweeping net. The composition of and several bioindices for the hexapods were determined, and multivariate analyses were conducted to explore the dynamics and seasonal distribution of the hexapods. A total of 32,000 individuals belonging to 11 orders and 113 families were collected, with adult individuals being collected more frequently in warmer seasons (from June to October). Of the sampled individuals, 90% belonged to the orders Collembola (42%), Hemiptera (35%), and Hymenoptera (13%). The number of individuals belonging to Hemiptera were stable in all seasons, and the number of hymenopteran wasps was influenced by temperature and exhibited a stable dynamic pattern. The number of individuals belonging to Collembola fluctuated dramatically. The multivariate analyses revealed that the collected hexapods could be divided into two major family groups according to survey season (i.e., summer and winter groups). Several families were collected only in summer, but a few were collected only in winter. Eigenvalues obtained from a principal component analysis revealed that the families Chironomidae, Delphacidae, Entomobryidae, Hypogastruridae, Sminthuridae, and Thripidae (all dominant) were the major contributors to the winter group. These families were abundant all year, although some were more abundant during winter. The three dominant orders Collembola, Hemiptera, and Hymenoptera, each of which has a distinct community structure and dynamic pattern, may have their own adaptive mechanisms in the subtropical regions of Taiwan. Hemiptera individuals, which feed on YB, were most abundant in the adult stage in summer and in the nymphal stage in winter. The abundance of parasitic hymenopteran wasps, which had stable dynamic patterns, was associated with that of their host insects and temperature. The drastic fluctuations in the abundance of Collembola may have been caused by abiotic factors, such as precipitation and microhabitat factors. The early onset of spring and the late onset of winter might also affect the dynamics of the studied hexapods.

Highly accurate long reads are crucial for realizing the potential of biodiversity genomics.

BACKGROUND: Generating the most contiguous, accurate genome assemblies given available sequencing technologies is a long-standing challenge in genome science. With the rise of long-read sequencing, assembly challenges have shifted from merely increasing contiguity to correctly assembling complex, repetitive regions of interest, ideally in a phased manner. At present, researchers largely choose between two types of long read data: longer, but less accurate sequences, or highly accurate, but shorter reads (i.e., >Q20 or 99% accurate). To better understand how these types of long-read data as well as scale of data (i.e., mean length and sequencing depth) influence genome assembly outcomes, we compared genome assemblies for a caddisfly, Hesperophylax magnus, generated with longer, but less accurate, Oxford Nanopore (ONT) R9.4.1 and highly accurate PacBio HiFi (HiFi) data. Next, we expanded this comparison to consider the influence of highly accurate long-read sequence data on genome assemblies across 6750 plant and animal genomes. For this broader comparison, we used HiFi data as a surrogate for highly accurate long-reads broadly as we could identify when they were used from GenBank metadata. RESULTS: HiFi reads outperformed ONT reads in all assembly metrics tested for the caddisfly data set and allowed for accurate assembly of the repetitive ~ 20 Kb H-fibroin gene. Across plants and animals, genome assemblies that incorporated HiFi reads were also more contiguous. For plants, the average HiFi assembly was 501% more contiguous (mean contig N50 = 20.5 Mb) than those generated with any other long-read data (mean contig N50 = 4.1 Mb). For animals, HiFi assemblies were 226% more contiguous (mean contig N50 = 20.9 Mb) versus other long-read assemblies (mean contig N50 = 9.3 Mb). In plants, we also found limited evidence that HiFi may offer a unique solution for overcoming genomic complexity that scales with assembly size. CONCLUSIONS: Highly accurate long-reads generated with HiFi or analogous technologies represent a key tool for maximizing genome assembly quality for a wide swath of plants and animals. This finding is particularly important when resources only allow for one type of sequencing data to be generated. Ultimately, to realize the promise of biodiversity genomics, we call for greater uptake of highly accurate long-reads in future studies.

Functional characterization of maternally accumulated hydrolases in the mature oocytes of the vector Rhodnius prolixus reveals a new protein phosphatase essential for the activation of the yolk mobilization and embryo development.

Yolk biogenesis and consumption have been well conserved in oviparous animals throughout evolution. Most egg-laying animals store yolk proteins within the oocytes' yolk granules (Ygs). Following fertilization, the Ygs participate in controlled pathways of yolk breakdown to support the developing embryo's anabolic metabolism. While the unfolding of the yolk degradation program is a crucial process for successful development in many species, the molecular mechanisms responsible for yolk mobilization are still mysterious and have mostly not been explored. Here, we investigate the functional role of the oocyte maternally accumulated mRNAs of a protein phosphatase (PP501) and two aspartic proteases (cathepsin-D 405, CD405 and cathepsin-D 352, CD352) in the yolk degradation and reproduction of the insect vector of Chagas disease Rhodnius prolixus. We found that PP501 and CD352 are highly expressed in the vitellogenic ovary when compared to the other organs of the adult insect. Parental RNAi silencing of PP501 resulted in a drastic reduction in oviposition and increased embryo lethality whereas the silencing of CD352 resulted only in a slight decrease in oviposition and embryo viability. To further investigate the PP501-caused high reproduction impairment, we investigated the Ygs biogenesis during oocyte maturation and the activation of the yolk degradation program at early development. We found that the Ygs biogenesis was deficient during oogenesis, as seen by flow cytometry, and that, although the PP501-silenced unviable eggs were fertilized, the Ygs acidification and acid phosphatase activity were affected, culminating in a full impairment of the yolk proteins degradation at early embryogenesis. Altogether we found that PP501 is required for the oocyte maturation and the activation of the yolk degradation, being, therefore, essential for this vector reproduction.

Diversity, Form, and Postembryonic Development of Paleozoic Insects.

While Mesozoic, Paleogene, and Neogene insect faunas greatly resemble the modern one, the Paleozoic fauna provides unique insights into key innovations in insect evolution, such as the origin of wings and modifications of postembryonic development including holometaboly. Deep-divergence estimates suggest that the majority of contemporary insect orders originated in the Late Paleozoic, but these estimates reflect divergences between stem groups of each lineage rather than the later appearance of the crown groups. The fossil record shows the initial radiations of the extant hyperdiverse clades during the Early Permian, as well as the specialized fauna present before the End Permian mass extinction. This review summarizes the recent discoveries related to the documented diversity of Paleozoic hexapods, as well as current knowledge about what has actually been verified from fossil evidence as it relates to postembryonic development and the morphology of different body parts.

Extracting abundance information from DNA-based data.

The accurate extraction of species-abundance information from DNA-based data (metabarcoding, metagenomics) could contribute usefully to diet analysis and food-web reconstruction, the inference of species interactions, the modelling of population dynamics and species distributions, the biomonitoring of environmental state and change, and the inference of false positives and negatives. However, multiple sources of bias and noise in sampling and processing combine to inject error into DNA-based data sets. To understand how to extract abundance information, it is useful to distinguish two concepts. (i) Within-sample across-species quantification describes relative species abundances in one sample. (ii) Across-sample within-species quantification describes how the abundance of each individual species varies from sample to sample, such as over a time series, an environmental gradient or different experimental treatments. First, we review the literature on methods to recover across-species abundance information (by removing what we call "species pipeline biases") and within-species abundance information (by removing what we call "pipeline noise"). We argue that many ecological questions can be answered with just within-species quantification, and we therefore demonstrate how to use a "DNA spike-in" to correct for pipeline noise and recover within-species abundance information. We also introduce a model-based estimator that can be used on data sets without a physical spike-in to approximate and correct for pipeline noise.