Effects of mercury at field estimated concentration in brain of Bombus atratus (Hymenoptera: Bombini).

Although mercury neurotoxic effects are well known in several species, it is poorly studied in bees. Mercury contamination is increasing in several regions of the Brazilian Amazon Rainforest due to illegal and indiscriminate gold mining. Therefore, this study aimed to evaluate the effects of mercury (Hg) in brain Kenyon cells of foraging workers of Bombus atratus exposed to an average concentration (110 ppb) found in pots of honey from native bees of South America and Australia. Twenty forager workers were collected in the field (23° 34' S 47° 31' W), divided into control (n = 10) and exposed (n = 10) groups, and individually kept in special boxes for 48 h. For the exposed group, we offered Hg solution (at 110 ppb) ad libitum, while for the control group we offered water, and for both sucrose syrup at 70%. After the exposure time, the bees were crio-anesthezied at 4 °C. Brains were dissected and processed for morphological, morphometric, and histochemical analyses. Morphological results showed that the Kenyon cells of the Hg-exposed group presented both cytoplasmic vacuolization and nuclear pyknosis, which indicate cell death. These findings were corroborated by the acridine orange staining. Hg exposure also induced significant nuclear chromatin compaction in Kenyon cells. The calyces and peduncles of the mushroom bodies showed disorganization and vacuolization. In summary, these changes may imply in a severe impairment of the cognitive abilities of the bees, which could lead them to the loss of many tasks, such as foraging or even nest founding by the queen.

Octopamine and Dopamine differentially modulate the nicotine-induced calcium response in Drosophila Mushroom Body Kenyon Cells.

In Drosophila associative olfactory learning, an odor, the conditioned stimulus (CS), is paired to an unconditioned stimulus (US). The CS and US information arrive at the Mushroom Bodies (MB), a Drosophila brain region that processes the information to generate new memories. It has been shown that olfactory information is conveyed through cholinergic inputs that activate nicotinic acetylcholine receptors (nAChRs) in the MB, while the US is coded by biogenic amine (BA) systems that innervate the MB. In this regard, the MB acts as a coincidence detector. A better understanding of the properties of the responses gated by nicotinic and BA receptors is required to get insights on the cellular and molecular mechanisms responsible for memory formation. In recent years, information has become available on the properties of the responses induced by nAChR activation in Kenyon Cells (KCs), the main neuronal MB population. However, very little information exists on the responses induced by aminergic systems in fly MB. Here we have evaluated some of the properties of the calcium responses gated by Dopamine (DA) and Octopamine (Oct) in identified KCs in culture. We report that exposure to BAs induces a fast but rather modest increase in intracellular calcium levels in cultured KCs. The responses to Oct and DA are fully blocked by a VGCC blocker, while they are differentially modulated by cAMP. Moreover, co-application of BAs and nicotine has different effects on intracellular calcium levels: while DA and nicotine effects are additive, Oct and nicotine induce a synergistic increase in calcium levels. These results suggest that a differential modulation of nicotine-induced calcium increase by DA and Oct could contribute to the events leading to learning and memory in flies.