Assessment of soil fertility and nutrient management strategies in calcareous soils of Khuzestan province: a case study using the Nutrient Index Value method.

Soil fertility (SF) is a crucial factor that directly impacts the performance and quality of crop production. To investigate the SF status in agricultural lands of winter wheat in Khuzestan province, 811 samples were collected from the soil surface (0-25 cm). Eleven soil properties, i.e., electrical conductivity (EC), soil organic carbon (SOC), total nitrogen (TN), calcium carbonate equivalent (CCE), available phosphorus (Pav), exchangeable potassium (Kex), iron (Fe), copper (Cu), zinc (Zn), manganese (Mn), and soil pH, were measured in the samples. The Nutrient Index Value (NIV) was calculated based on wheat nutritional requirements. The results indicated that 100%, 93%, and 74% of the study areas for CCE, pH, and EC fell into the low, moderate, and moderate to high NIV classes, respectively. Also, 25% of the area is classified as low fertility (NIV < 1.67), 75% falls under medium fertility (1.67 < NIV value < 2.33), and none in high fertility (NIV value > 2.33). Assessment of the mean wheat yield (AWY) and its comparison with NIV showed that the highest yield was in the Ramhormoz region (5200 kg.ha-1), while the lowest yield was in the Hendijan region (3000 kg.ha-1) with the lowest EC rate in the study area. Elevated levels of salinity and CCE in soils had the most negative impact on irrigated WY, while Pav, TN, and Mn availability showed significant effects on crop production. Therefore, implementing SF management practices is essential for both quantitative and qualitative improvement in irrigated wheat production in Khuzestan province.

First Morphological and Molecular Characterization of Paratylenchus vandenbrandei (Rhabditida: Tylenchulidae) in Iran.

Paratylenchus vandenbrandei, has been recovered from the rhizospheric soil of Euphrates poplar (Populus euphratica) in the Karkheh protected area of Khuzestan province, southwestern Iran. The species was identified as P. vandenbrandei by the presence of three lines in the lateral fields; conoid rounded lip region; presence of submedian lobes, a stylet 24.0-28.8 µm long; an excretory pore at the level of the anterior part of the pharyngeal bulb; a round-to-oval spermatheca; presence of vulval flaps; and a conoid tail, with a terminus that is rounded or slightly pointed in some specimens. Males have a conoid tail, with a rounded-to-slightly-pointed terminus. The phylogenetic relationships of the species were reconstructed and investigated using partial sequencing of the D2-D3 expansion segments of large subunits, as well as internal transcribed spacer regions (LSU D2-D3 and ITS rDNA) based on Bayesian inference (BI). P. vandenbrandei has formed a clade with P. neonanus, P. minor, P. nainianus, P. chongqinjensis, P. pedrami, P. baldaccii, P. leptos and P. rostrocaudatus with maximal support (BPP = 1.00). To the best of our knowledge, this is the first report of P. vandenbrandei in Iran and the first molecular characterization of the species worldwide.

Prevalence of asymptomatic strongyloidiasis co-infection in COVID-19 patients residing in endemic areas.

BACKGROUND: Fatal forms of strongyloidiasis, hyperinfection syndrome (HS) and disseminated strongyloidiasis (DS), are caused by exaggerated autoinfection of the intestinal nematode, Strongyloides stercoralis (S. stercoralis). Corticosteroids, frequently administered to patients with severe COVID-19, can transform chronic asymptomatic strongyloidiasis into the above-mentioned fatal diseases. This study aimed to investigate the prevalence of strongyloidiasis in COVID-19 patients receiving corticosteroids in a hypoendemic region. METHODS: The present cross-sectional study enrolled 308 COVID-19 patients admitted to two hospitals in Ahvaz and Abadan in the southwest of Iran between 2020 and 2022. A real-time reverse transcription polymerase chain reaction (RT-PCR) test and chest computed tomography (CT) scan were employed to detect and monitor the disease's severity in the patients, respectively. All patients were evaluated for IgG/IgM against S. stercoralis using Enzyme-linked immunosorbent assay (ELISA) test. Subsequently, individuals with a positive ELISA test were confirmed using parasitological methods, including direct smear and agar plate culture (APC). RESULTS: The patients were between 15 and 94 years old, with a mean age of 57.99 ± 17.4 years. Of the 308 patients, 12 (3.9%) had a positive ELISA test, while 296 (96.1%) had negative results. Three of the 12 patients with a positive ELISA result died, and three failed to provide a stool sample. To this end, only six cases were examined parasitologically, in which S. stercoralis larvae were observed in five patients. Significant differences were found between S. stercoralis infection with sex (p = 0.037) and age (p = 0.027). Binary regression analysis revealed that strongyloidiasis was positively associated with sex (odds ratio [OR]: 5.137; 95% confidence interval [CI]: 1.107-23.847), age (OR: 5.647; 95% CI 1.216-26.218), and location (OR: 3.254; 95% CI: 0.864-12.257). CONCLUSIONS: Our findings suggest that screening for latent strongyloidiasis in COVID-19 patients in endemic areas using high-sensitivity diagnostic methods, particularly ELISA, before receiving suppressive drugs should be given more consideration.

Assessing and mapping distribution, area, and density of riparian forests in southern Iran using Sentinel-2A, Google earth, and field data.

Riparian forests in Iran are valuable ecosystems which have many ecological values. Because of destruction of these forests in recent years, providing spatio-temporal information from area and distribution of these ecosystems has been receiving much attention. This study was performed for mapping distribution, area and density of riparian forests in southern Iran using Sentinel-2A, Google Earth, and field data. First Sentinel-2A satellite image of the study area was provided. The field work was performed to take the training areas and to assess the forest density of riparian forests in Khuzestan province. In the first part of this study, after selecting training areas as pixel-based samples on the Sentinel-2A satellite image, supervised classification of image was performed using support vector machine (SVM) algorithm to classify the distribution of riparian forests. After classification of Sentinel-2A satellite image, the boundary of riparian forests map was checked and corrected on Google Earth images. In the second part of this study, field data, Normalized Difference Vegetation Index (NDVI), and regression model were used to assess the density of riparian forests. Finally, the accuracy of the final riparian forest map (showing both distribution and density of riparian forests) was assessed using Google Earth images. Results showed that the final riparian forest map (showing both distribution and density of riparian forests) with overall accuracy 89% and kappa index 0.81 had a good accuracy for classifying the distribution and density of riparian forests in Khuzestan province. These results demonstrate the accuracy of SVM algorithm for classifying the distribution of riparian forests and also capability of NDVI for classifying the density of riparian forests in this study. Results also showed that regression model (R2 = 0.97) is reliable for estimating riparian forest density. The results demonstrated that there are 68447.18 ha of riparian forest around the main rivers in Khuzestan province, mainly distributed in the northwest and southeast of the province. From this area, 54694.15 ha have been covered by dense forests and 13753.03 ha by sparse forests. Results of this research have created the useful data of area, distribution and density of riparian forests in 10-m spatial resolution which is necessary for conservation and management of these forests in southern Iran. It is suggested that mapping area, distribution and density of these forests would be performed using SVM algorithm and NDVI in the certain temporal periods for protective management of these ecosystems in time series.

Mineralogical, geochemical, and textural characteristics of soil and airborne samples during dust storms in Khuzestan, southwest Iran.

Dust storms are frequent phenomena in Khuzestan Province, southwest Iran, leading to environmental hazards and deleterious impacts on human health. This work analyzes mineralogic and geochemical characteristics of dust sediments at the source regions and in deposition areas in southwest Iran during three dust-storm events in winter and spring 2018. Twenty soil and airborne dust samples were collected and analyzed for compositions of dust at different distances from the source regions in Iraq and southwest Iran, aiming to assess the source characterization and possible mixing processes in the atmosphere. The grain size distributions were also analyzed at specific sites. The results show that about 50 % of the volume size distribution corresponds to particle sizes of above 20 µm, indicating local/regional dust storms of coarse to giant particles. XRD analysis indicates that calcite is the dominant mineral in all the samples, with a high quartz and dolomite fraction. The most abundant major compounds are SiO2 and CaO, while Cl, Ba, Sr, Pb, Ni, Zn, Cr, V are the main trace elements. The enrichment factor (EF) analysis showed that apart from Th, Nb, Ce, and V, all the other elements (Pb, Zn, Cr, etc.) have an anthropogenic origin or represent high amounts of pollutant contamination. High levels of elemental enrichment are attributable to intensive pollution in Khuzestan Province and at sampling sites due to fossil-fuel combustion, gas and petroleum drilling activities. Moreover, based on the geo-accumulation index (Igeo), all samples are found to be contaminated by heavy metals due to prior war-related materiel, oil and gas extraction, and emissions from polluting industries.

Effects of climate variables on the incidence of scorpion stings in Iran for five years.

BACKGROUND: Although scorpionism is recorded worldwide, some regions such as Iran present a higher incidence. Due to the great prevalence of scorpion stings in Khuzestan province, southwestern Iran, the present study examined the relationship between different climate parameters and the scorpion sting rate in this area from April 2010 to March 2015. METHODS: In this cross-sectional descriptive-analytical study, we considered all scorpion sting cases recorded in the Department of Infectious Diseases, Ahvaz Jundishapur University of Medical Sciences. Data were analyzed using statistics, frequency distribution and Pearson's correlation coefficient. RESULTS: A total of 104,197 cases of scorpion stings was recorded from 2010 to 2015. The cumulative incidence of scorpion sting was 2.23%. The spatial distribution of scorpion stings showed that most cases occurred in the Dehdez district (4,504 scorpion stings/100,000 inhabitants) and the Masjed Soleyman county (4,069 scorpion stings/100,000 inhabitants). A significant association was found between climate factors (temperature, evaporation rate, sunshine duration, humidity, and precipitation) and the scorpion sting rate. An increase in rainfall and humidity coincided with a reduction in scorpion stings whereas an increase in temperature, evaporation, and sunshine duration was accompanied by a growth of scorpion stings. No significant correlation was found between wind velocity/direction and the incidence rate of stings. Moreover, the seasonal peak incidence of scorpion stings was recorded in summer (an average of 8,838 cases) and the lowest incidence was recorded during winter (an average of 1,286 cases). The annual trend of scorpion sting cases decreased during the period from 2010 to 2015. CONCLUSION: Climate variables can be a good index for predicting the incidence of scorpion stings in endemic regions. Since they occur mostly in the hot season, designing preventive measures in the counties and districts with a high incidence of scorpion stings such as Dehdez and Masjed Soleyman can minimize mortality and other burdens.

Effects of climate variables on the incidence of scorpion stings in Iran for five years

Background: Although scorpionism is recorded worldwide, some regions such as Iran present a higher incidence. Due to the great prevalence of scorpion stings in Khuzestan province, southwestern Iran, the present study examined the relationship between different climate parameters and the scorpion sting rate in this area from April 2010 to March 2015. Methods: In this cross-sectional descriptive-analytical study, we considered all scorpion sting cases recorded in the Department of Infectious Diseases, Ahvaz Jundishapur University of Medical Sciences. Data were analyzed using statistics, frequency distribution and Pearson's correlation coefficient. Results: A total of 104,197 cases of scorpion stings was recorded from 2010 to 2015. The cumulative incidence of scorpion sting was 2.23%. The spatial distribution of scorpion stings showed that most cases occurred in the Dehdez district (4,504 scorpion stings/100,000 inhabitants) and the Masjed Soleyman county (4,069 scorpion stings/100,000 inhabitants). A significant association was found between climate factors (temperature, evaporation rate, sunshine duration, humidity, and precipitation) and the scorpion sting rate. An increase in rainfall and humidity coincided with a reduction in scorpion stings whereas an increase in temperature, evaporation, and sunshine duration was accompanied by a growth of scorpion stings. No significant correlation was found between wind velocity/direction and the incidence rate of stings. Moreover, the seasonal peak incidence of scorpion stings was recorded in summer (an average of 8,838 cases) and the lowest incidence was recorded during winter (an average of 1,286 cases). The annual trend of scorpion sting cases decreased during the period from 2010 to 2015. Conclusion: Climate variables can be a good index for predicting the incidence of scorpion stings in endemic regions. Since they occur mostly in the hot season, designing preventive measures in the counties and districts with a high incidence of scorpion stings such as Dehdez and Masjed Soleyman can minimize mortality and other burdens.(AU)

Morphological and molecular data of two species of the rare genera Thada Thorne, 1941 and Tenunemellus Siddiqi, 1986 (Nematoda: Tylenchidae) from Iran.

Thada populus n. sp. was found in the rhizosphere of Populus euphratica in the city of Dezful, south-western Iran. The new species is characterized by its 365-453 µm body length, cuticle with transverse annuli 0.9-1.2 µm wide, lateral fields with four lines, lip region low with one or two annuli, 7.0-8.5 µm wide at base, longitudinal or slightly sigmoid amphidial slit, delicate stylet 8.4-9.8 µm long with posteriorly directed knobs, dorsal pharyngeal gland opening at 1.0-1.5 µm posterior to the stylet knobs, non-muscular and valve-less median bulb, pyriform and offset basal bulb, almost oval spermatheca, posterior position of vulva (V = 75-79%) without lateral membrane, short post-vulval uterine sac and conical tail with finely to broadly rounded terminus. Morphological differences of the new species with those of the type species, Thada striata, are discussed. Molecular phylogenetic studies of the new species using partial sequences of small subunit ribosomal DNA revealed that the new species formed a clade with Malenchus spp., Filenchus facultativus, F. fungivorus and Filenchus sp. in Bayesian inference. Morphological and molecular phylogenetic studies were also performed on Tenunemellus indicus, the second recovered species, the latter analysis using partial sequences of large subunit ribosomal DNA. In the inferred Bayesian tree, T. indicus formed a clade with Labrys fuzhouensis, Lelenchus leptosoma from the Netherlands, Malenchus spp. and Filenchus discrepans.

Labrys khuzestanensis n. sp. (Nematoda, Tylenchidae), a new member of the genus with large labial plate.

Labrys khuzestanensis n. sp. was recovered from the rhizosphere of a palm tree in southwestern Iran and described using morphological and molecular data. The new species was characterized by combination of the following features: its 590-675 µm long body, finely annulated cuticle in light microscopy (LM), lateral field with two smooth incisures, cephalic region dorso-ventrally flattened with protuberant, elongate and laterally extended labial plate, appearing a V-shaped piece in lateral view, amphidial openings as longitudinal slits confined to labial plate in scanning electron microscopic (SEM) images, stylet 7.5-9.0 µm long, elongated fusiform median bulb with faint valvular apparatus, excretory pore wide with moderately sclerotized duct and elongate filiform tail with finely rounded tip. Compared to four currently known species under the genus, the new species has a remarkably larger and more prominent labial plate as revealed by SEM and trapezoid cephalic region under LM. The molecular phylogenetic analyses of the new species using partial SSU rDNA revealed it has close affinity with three species of Labrys (L. chinensis, L. fuzhouensis and L. filiformis) in Bayesian inference (BI) and maximum likelihood (ML) methods and occupied a placement inside their clade. L. fujianensis, the other species of the genus, occupied a distant placement to the aforementioned clade. This is the second species of Labrys being originally described from Iran, representing the third species of Labrys occurring in the country.

Gill lesions and mortality in common carp (Cyprinus carpio) with a dense bloom of Heterosigma-like algae in Khuzestan province.

BACKGROUND: Widespread common carp (Cyprinus carpio) mortalities have been recorded in Khuzestan province fish farms in recent years. In summer of 2017 two cases of harmful algal bloom were encountered that led to massive mortality in common carp in Khuzestan, Iran. AIMS: The aim of this study was to identify the possible etiologic agent of two mortalities with characteristic symptoms of gill lesions due to harmful algae. METHODS: Water samples were collected and 5 moribund fishes were examined by histophatologic, scanning electron microscopic and PCR examination. RESULTS: In wet smear preparations, a lot of algal cells and fragments, and sloughed, necrotic epithelial cells were observed between the lamellae. In histopathologic examination of gills, hyperplasia, necrosis and algal cells surrounded by hyperplastic cells were seen in tissue sections. No inflammatory cell aggregation was noticed. In scanning electron microscopic examination the algae was found attached to the gill surface (cell diameter: 8.5 ± 4.2 µm) with 2 equal flagella. CONCLUSION: Phytoplankton analysis using direct microscopy and electron microscopy, morphologically resembling Heterosigma was identified, however, in PCR tests, Heterosigma analysis showed negative results, therefore the causative agent was called "Heterosigma-like" algae.

Analysis of knowledge and attitudes related to parasitic infections among inhabitants of Ahvaz County, Khuzestan Province, Iran.

Parasitic diseases are among the major health problems of various societies, especially people in developing countries, causing high economic and mortality burdens. Many researchers have reported that awareness and knowledge of a disease are effective in preventing and controlling the disease. The aim of the present study was to assess the knowledge and attitudes on parasitic diseases of the population in Ahvaz County, southwestern Iran. This community-based cross-sectional study was carried out in 2017 on 3500 subjects above 15 years old of Ahvaz County. First, participants were provided the necessary information regarding the study, and then data were collected using a constructed questionnaire. Of the 3500 participants, 1732 (49.5%) were female and 1768 (50.5%) were male, 348 (9.9%) said that they had been infected with a parasitic disease at least once in their lifetime. Almost half of the participants (42.8%) knew malaria was a parasitic disease and 50.2%, 48.9%, and 41.5% were aware of the possible transmission of parasitic diseases through uncooked meat, dirty hands, and close contact with animals. Regarding clinical symptoms, 73.2% and 32.6% of the respondents knew diarrhea and abdominal pain respectively were clinical symptoms of intestinal parasitic diseases. However, only 31.3% were aware of the close contact with cats and the risk of human toxoplasmosis and possible abortion in pregnant women. The findings reveal that the attitude of most participants toward treatment was positive (93.9%), but only 47.5% of believed that disinfecting fruits and vegetables could prevent intestinal parasitic infections.

Human Rhinoviruses A9, A49, B14 and Echovirus 3, 9 among the patients with acute respiratory infection.

  Background: Acute respiratory infection result in high mortality and morbidity worldwide. There are several viral factors that originate respiratory diseases among them Enteroviruses(EVs) and Human Rhinoviruses(HRVs) can be mentioned. HRVs and EVs belong to Picornaviridae family and they have been recently classified under Enteroviruses. The pattern of respiratory infections generating organisms varies according to geographical locations. Therefore, it seems necessary to organize an appropriate plan to manage common viral diseases exclusively about Rhinoviruses and Enteroviruses. PATIENT AND METHODS: A total of 100 samples were collected from patients with acute respiratory infections (ARIs) who were hospitalized in Ahvaz city hospitals during December 2012 to November 2013 (one year longitude). Semi-Nested PCR was done on samples for detection of HRVs and EVs using region gene of VP4/VP2. Phylogenetic and molecular evolutionary analyses performed with MEGA version 5 software find out the sequence homology among the detected HRV and EV serotype. RESULTS: The results of this study revealed that from of 100 cases of ARIs 19 patients (19%) were HRV positive and 3 (3%) patients positive for EVs. Most positive cases of HRVs were observed in the autumn season while 3 positive cases of EVs were equally found in spring, summer and autumn. Phylogenetic analyses showed that the HRV strains were HRV-A9, HRV-A49, HRV-B14 and EV strains were Echo3 and 9. CONCLUSION: The results of this study revealed that high prevalence of 19% HRVs, HRV-A9, HRV-A49, HRV-B14 serotypes and low frequency of 3% Echo Viruses, Echo3 and Echo 9 serotypes have been detected in patients with ARI.

Genomic analysis of Blastocystis hominis isolates in patients with HIV-positive using locus SSU-rDNA.

Blastocystis hominis (B. hominis) is a protozoan zoonosis which clinical signs of infection with this parasite has been reported to be more severe in patients with weakened immune systems than healthy controls. So, the aim of the study was to evaluate genomic analysis of B. hominis isolates obtained from patients with HIV-positive using locus SSU-rDNA. At first, 268 stool samples were randomly collected from patients with HIV-positive referred to health centers of Khuzestan province, southwest of Iran. Formol-ether and direct smear techniques were used for the detection of parasitic agents. After extracting DNA, the samples were analyzed by the PCR method. Finally, the subtypes were determined by the sequencing and PCR methods. New samples were used for the preparation of positive control sample; they were cultured in coagulant-serum biphasic cultivation media. Of 268 stool samples, 33 (12.3%) cases were detected positive for B. hominis using Formol-Ether technique but 51 (19%) cases were positive using molecular method. The most common isolates were related to the subtype III with 29 positive cases (56.8%), then, genotype I with 11 (21.6%) cases, 6 cases (11.8%) with genotype II, 3 (5.9%) combined cases with genotypes I and III as well as 2 cases (3.9%) with genotype VI. There was a significant difference between two groups of HIV-positive patients (infected with the parasite and/or without the parasite) in the term of the mean of TCD4-positive cells. The results indicated a relatively high prevalence of B. hominis in HIV-positive patients as well as our findings may represent that the number reduction of TCD4-positive cells has an effective role in the increased risk of the parasitic infection in HIV-positive patients.

Evaluation of serum levels of C3 and C4 complement factors in patients with beta thalassemia major in Khuzestan Province, Southwest Iran.

BACKGROUND AND OBJECTIVES: Thalassemia syndrome is the most common genetic disorder in the world and infection is the second cause of death in these patients. Measurement of serum C3 and C4 complement factors in serum was done in 60 patients with beta thalassemia major in comparison with 30 healthy subjects as control group. MATERIALS AND METHODS: The serum level of C3 and C4 complement factors in 60 patients with beta thalassemia major who were randomly selected from among the patients referred to Shafa Hospital of Ahvaz was evaluated and compared with 30 samples from healthy individuals with no history of recent infectious or autoimmune diseases. It should be noted that single-radial-immunodiffusion assay was used in this study. RESULTS: This study has shown a significant reduction in serum levels of C3 and C4 in patients compared to controls (P value < 0.05). DISCUSSION: Decreased synthesis or increased consumption of complement factors in patients receiving multiple blood transfusions might lead to continuous contact between the immune system and various antigens, causing nonstop use of complement factors, recurrent infections, changes in parameters of the immune system due to iron overload as well as exposure to infectious factors such as HBV, HCV, HIV, and HTLV through blood transfusion.

Description of Enchodorus yeatsi n. sp. (Dorylaimida, Nordiidae) from Southern Iran and Its Molecular Phylogenetic Study.

Enchodorus yeatsi n. sp., a new species of the rare genus Enchodorus is described and illustrated based on its morphological and molecular characters. It was recovered from southern Iran. Females of the new species are characterized by having 1,511.3- to 1,792.5-µm long slender body, angular lip region having prominent papillae, 12- to 13-µm long odontostyle, double guiding ring, simple rod-like odontophore, didelphic-amphidelphic reproductive system, and 102- to 160-µm long elongate-conoid tail with rounded tip. Males of the new species are abundant and functional, characterized by 1,484- to 1,576-µm long body, 40- to 43-µm long spicules, 5 to 6 ventromedian supplements, and ventrally bent elongate conical tail. Compared to the type species, Enchodorus dolichurus, the new species has differences in its tail morphology and V value. These morphological differences and the separation of two species was further supported with basic differences in sequences of 28S rDNA D2/D3 and internal transcribed spacer 1 (ITS1) fragments. Compared to Enchodorus neodolichurus, it has basic differences in tail characters and spicule lengths. Molecular phylogenetic studies using partial sequences of 28S rDNA D2/D3 fragment of the new species and available sequences of Nordiidae members and several other dorylaim species/genera, revealed E. yeatsi n. sp. and E. dolichurus forming a clade with 0.81 Bayesian posterior probability (BPP). This clade forms a sister clade to the clade of Heterodorus sp. and Rhyssocolpus vinciguerrae, again with 0.81 BPP. In ITS1 tree, reconstructed using few available sequences, the new species and E. dolichurus formed a clade with 0.98 BPP.

Assessment of oxytetracycline and tetracycline antibiotics in manure samples in different cities of Khuzestan Province, Iran.

Tetracyclines (TCs), a class of antibiotics with a broad spectrum, are the most frequently used antibiotics in animal production. The major concern is that the widespread use of the antibiotics may lead to the emergence of new strains of bacteria that are resistant to these antibiotics. The objective of this study was to determine the residual levels of oxytetracycline and tetracycline in 80 animal manure samples that were collected from the livestock and poultry feedlots in Khuzestan Province. The residual levels of the antibiotics in the samples were extracted by using solid-phase extraction (SPE) method and subsequently were measured by liquid chromatography. Recoveries from the spiked poultry manure samples ranged from 65 to 113% for tetracycline and 86 to 132% for oxytetracycline. Relative standard deviations of the recoveries were less than 5.7% within the same day. Method detection limit (MDL) measured for oxytetracycline and tetracycline in the manure were 0.011 and 0.01 mg/kg, respectively. Analysis of the collected 50 chickens and 30 cow manure samples showed that the highest concentration of tetracycline was related to Behbahan City (5.36 mg/kg) and the lowest concentration was detected for Ramhormoz (0.05 mg/kg). The highest and lowest concentrations of oxytetracycline were respectively observed for Behbahan (13.77 mg/kg) and Ramhormoz (0.047 mg/kg). Based on the results, in chicken manure, there was significant statistical difference between the residual TC concentrations among five cities (p(value) < 0.05). However, no significant relationship was observed between oxytetracyclin (OTC) residual concentrations among five cities (p(value) > 0.05).