RESUMO
Aphelenchoides pseudogoodeyi has recently been reported in association with seeds of forage grasses and rice in Brazil and senescent strawberry plants, in the United States. This nematode is likely a mycophagous species; however, so far, its pathogenicity potential to plants is unclear. This study aimed to verify the pathogenicity of A. pseudogoodeyi to two species of ornamental plants. The experiments were conducted by inoculating A. pseudogoodeyi onto Bird's-Nest Fern (Asplenium nidus) and Oriental Lily (Lilium speciosum) leaves, using two inoculation methods (with and without injury). After 40 days of inoculation (DAI) in Bird's-Nest Fern and 5, 10, 20 and 40 DAI in Oriental Lily, the pathogenicity and the host efficiency were evaluated by symptoms observation and by severity, final nematode population and reproductive factor (RF), respectively. Additionally, a histopathological study was performed by inoculating A. pseudogoodeyi onto Bird's-Nest Fern for observing anatomical alterations. A. pseudogoodeyi was able to cause local necrotic lesions on both Bird's-Nest Fern and Oriental Lily leaves. However, the presence of injury was essential to enable A. pseudogoodeyi to penetrate and cause those symptoms in both plant species. Also, the total population of A. pseudogoodeyi decreased drastically over time and RF was <1, which characterized these species as poor-host or resistant plants. A. pseudogoodeyi penetrated into the foliar tissue and induced a total destruction of the mesophyll and collapse of the cells, with the formation of large intercellular spaces. It is concluded that A. pseudogoodeyi is an opportunistic pathogen as injury was required to induce symptoms in Bird's-Nest Fern and Oriental Lily.
RESUMO
In vertebrates such as the mouse and the human, primordial germ cells (PGCs) arise at the base of the allantois and are carried to the epithelium of the posterior intestine, to later migrate to the primordial gonads. In the case of bats, almost nothing is known about this process. To clarify the dynamics of PGCs during gonadal morphogenesis in the phyllostomid bat Sturnira lilium, the proteins for the Ddx4, Sox9 and Mis genes were detected in the gonads of embryos at different stages of development. We identified 15 stages (St) of embryonic development in Sturnira lilium. We found that the formation of the genital ridge and the establishment of the undifferentiated gonad take place between stages 11 and 14. The onset of morphological differentiation in the gonad is first detected in the male gonads at St17. The first PGCs in meiosis are detected in the ovary at St19, whereas in the testicles, the PGCs were in mitotic arrest. Structural changes leading to testicular and ovarian development in Sturnira lilium are observed to be similar to those described for the mouse; however, differences will be established concerning the time taken for these processes to occur.
Assuntos
Quirópteros/embriologia , Células Germinativas , Gônadas/embriologia , Morfogênese , Animais , Western Blotting , RNA Helicases DEAD-box/genética , RNA Helicases DEAD-box/metabolismo , Feminino , Imunofluorescência , Camundongos , Microscopia ConfocalRESUMO
Exposure of bulbs to cold, a physiological phenomenon called vernalization, and bulb size are important factors in the production of lily bulbs and flowers. This study aimed to verify the influence of vernalization of bulbs on flowering cut lily plants, as well as the impact of size and shape of harvest on the production and quality of flowers and bulbs. In turn, the way the stems of the plants used for cut-flower production are cropped is of higher importance for the production of new flower bulbs. In this sense, the experiment was conducted in Viçosa, MG, in a greenhouse in a randomized block design, in split splot scheme with three replications, in which the vernalization periods (25, 35 and 45 days at 4 ± 1 C) constituted the plots; bulb sizes (diameters of 3.2-3.8 cm; 2.5-3.2 cm 1.9- and 2.5 cm), subplots and ways to harvest (full harvest of the stem at the required length for the commercial harvest of the flower, commercial stem harvest at the commercial length, maintaining 10cm of stem in the soil; removal of the floral buds as soon as their appearance is observed and harvest at the end of the season), the sub subplots. The bulbs were planted in beds, with 15 x 20 cm spacing. It was evaluated the number of plants that flowered and the number of flowers, the length and the diameter of the floral buds, fresh and dry weights, diameter and plant height as well as number, perimeter and amount of fresh and dry bulbs.There was a decrease in the plant height with the increase of the vernalization period and a reduction ofthe diameter of the planted bulbs, as well as of the number and the fresh and dry weights of the producedbuds. The production of flowers and buds in number, size and weight was directly proportional to thesize of the planted bulbs, while the form of harvest with removal of flower buds increased the number,the perimeter and the fresh and dry weights of the buds...(AU)
A exposição dos bulbos ao frio, fenômeno fisiológico denominado vernalização, e o tamanho do bulbo são importantes na produção de flores de lírio. Por sua vez, a forma de colheita das hastes das plantas utilizadas para corte de flor é preponderante na produção de bulbos filhos. Este estudo teve como objetivo verificar a influência da vernalização dos bulbos em plantas de lírio em flor, bem como o impacto do tamanho e forma da colheita na produção e qualidade de flores e bulbos. O experimento foi conduzido em Viçosa, MG casa de vegetação, em blocos casualizados, com parcelas sub-subdivididas, com três repetições, em que os períodos de vernalização (25, 35 e 45 dias, a 4± 1? C) constituíram as parcelas; os tamanhos de bulbo (diâmetros de 3,2- 3,8 cm; 2,5-3,2 cm e 1,9- 2,5 cm), as subparcelas e as formas de colheita (colheita total da haste no ponto de colheita comercial da flor; colheita da haste no ponto comercial, mantendo 10 cm da haste no solo; retirada do botão floral assim que seu surgimento foi observado e colheita no fim do ciclo vegetativo), as sub subparcelas. Os bulbos foram plantados em canteiros, em espaçamento de 15 x 20 cm. Foram avaliados o número de plantas que floresceram e número de flores, comprimento e diâmetro do botão floral, massas fresca e seca, diâmetro e altura da planta e número, perímetro e massa fresca e seca de bulbos. Houve decréscimo na altura da planta como aumento do período de vernalização e redução do diâmetro do bulbo plantado, assim como do númeroe da massa seca e fresca dos bulbos produzidos. Já, a produção de flores e bulbos, em número, tamanhoe peso, foi diretamente proporcional ao tamanho do bulbo plantado, enquanto a forma de colheita, comretirada do botão floral aumentou o número, o perímetro e as massas frescas e secas dos bulbos. Bulboscom diâmetro entre 3,2 - 3,8 cm, armazenados por 25 dias em câmara fria possibilitam maior produçãode flores e a maior altura da haste, fatores que favorecem o aumento do...(AU)
Assuntos
Bulbo/anatomia & histologia , Bulbo/química , Flores/crescimento & desenvolvimento , Lilium/crescimento & desenvolvimento , Produtos AgrícolasRESUMO
Exposure of bulbs to cold, a physiological phenomenon called vernalization, and bulb size are important factors in the production of lily bulbs and flowers. This study aimed to verify the influence of vernalization of bulbs on flowering cut lily plants, as well as the impact of size and shape of harvest on the production and quality of flowers and bulbs. In turn, the way the stems of the plants used for cut-flower production are cropped is of higher importance for the production of new flower bulbs. In this sense, the experiment was conducted in Viçosa, MG, in a greenhouse in a randomized block design, in split splot scheme with three replications, in which the vernalization periods (25, 35 and 45 days at 4 ± 1 C) constituted the plots; bulb sizes (diameters of 3.2-3.8 cm; 2.5-3.2 cm 1.9- and 2.5 cm), subplots and ways to harvest (full harvest of the stem at the required length for the commercial harvest of the flower, commercial stem harvest at the commercial length, maintaining 10cm of stem in the soil; removal of the floral buds as soon as their appearance is observed and harvest at the end of the season), the sub subplots. The bulbs were planted in beds, with 15 x 20 cm spacing. It was evaluated the number of plants that flowered and the number of flowers, the length and the diameter of the floral buds, fresh and dry weights, diameter and plant height as well as number, perimeter and amount of fresh and dry bulbs.There was a decrease in the plant height with the increase of the vernalization period and a reduction ofthe diameter of the planted bulbs, as well as of the number and the fresh and dry weights of the producedbuds. The production of flowers and buds in number, size and weight was directly proportional to thesize of the planted bulbs, while the form of harvest with removal of flower buds increased the number,the perimeter and the fresh and dry weights of the buds...
A exposição dos bulbos ao frio, fenômeno fisiológico denominado vernalização, e o tamanho do bulbo são importantes na produção de flores de lírio. Por sua vez, a forma de colheita das hastes das plantas utilizadas para corte de flor é preponderante na produção de bulbos filhos. Este estudo teve como objetivo verificar a influência da vernalização dos bulbos em plantas de lírio em flor, bem como o impacto do tamanho e forma da colheita na produção e qualidade de flores e bulbos. O experimento foi conduzido em Viçosa, MG casa de vegetação, em blocos casualizados, com parcelas sub-subdivididas, com três repetições, em que os períodos de vernalização (25, 35 e 45 dias, a 4± 1? C) constituíram as parcelas; os tamanhos de bulbo (diâmetros de 3,2- 3,8 cm; 2,5-3,2 cm e 1,9- 2,5 cm), as subparcelas e as formas de colheita (colheita total da haste no ponto de colheita comercial da flor; colheita da haste no ponto comercial, mantendo 10 cm da haste no solo; retirada do botão floral assim que seu surgimento foi observado e colheita no fim do ciclo vegetativo), as sub subparcelas. Os bulbos foram plantados em canteiros, em espaçamento de 15 x 20 cm. Foram avaliados o número de plantas que floresceram e número de flores, comprimento e diâmetro do botão floral, massas fresca e seca, diâmetro e altura da planta e número, perímetro e massa fresca e seca de bulbos. Houve decréscimo na altura da planta como aumento do período de vernalização e redução do diâmetro do bulbo plantado, assim como do númeroe da massa seca e fresca dos bulbos produzidos. Já, a produção de flores e bulbos, em número, tamanhoe peso, foi diretamente proporcional ao tamanho do bulbo plantado, enquanto a forma de colheita, comretirada do botão floral aumentou o número, o perímetro e as massas frescas e secas dos bulbos. Bulboscom diâmetro entre 3,2 - 3,8 cm, armazenados por 25 dias em câmara fria possibilitam maior produçãode flores e a maior altura da haste, fatores que favorecem o aumento do...
Assuntos
Bulbo/anatomia & histologia , Bulbo/química , Flores/crescimento & desenvolvimento , Lilium/crescimento & desenvolvimento , Produtos AgrícolasRESUMO
Exposure of bulbs to cold, a physiological phenomenon called vernalization, and bulb size are important factors in the production of lily bulbs and flowers. This study aimed to verify the influence of vernalization of bulbs on flowering cut lily plants, as well as the impact of size and shape of harvest on the production and quality of flowers and bulbs. In turn, the way the stems of the plants used for cut-flower production are cropped is of higher importance for the production of new flower bulbs. In this sense, the experiment was conducted in Viçosa, MG, in a greenhouse in a randomized block design, in split splot scheme with three replications, in which the vernalization periods (25, 35 and 45 days at 4 ± 1 C) constituted the plots; bulb sizes (diameters of 3.2-3.8 cm; 2.5-3.2 cm 1.9- and 2.5 cm), subplots and ways to harvest (full harvest of the stem at the required length for the commercial harvest of the flower, commercial stem harvest at the commercial length, maintaining 10cm of stem in the soil; removal of the floral buds as soon as their appearance is observed and harvest at the end of the season), the sub subplots. The bulbs were planted in beds, with 15 x 20 cm spacing. It was evaluated the number of plants that flowered and the number of flowers, the length and the diameter of the floral buds, fresh and dry weights, diameter and plant height as well as number, per
A exposição dos bulbos ao frio, fenômeno fisiológico denominado vernalização, e o tamanho do bulbo são importantes na produção de flores de lírio. Por sua vez, a forma de colheita das hastes das plantas utilizadas para corte de flor é preponderante na produção de bulbos filhos. Este estudo teve como objetivo verificar a influência da vernalização dos bulbos em plantas de lírio em flor, bem como o impacto do tamanho e forma da colheita na produção e qualidade de flores e bulbos. O experimento foi conduzido em Viçosa, MG casa de vegetação, em blocos casualizados, com parcelas sub-subdivididas, com três repetições, em que os períodos de vernalização (25, 35 e 45 dias, a 4± 1? C) constituíram as parcelas; os tamanhos de bulbo (diâmetros de 3,2- 3,8 cm; 2,5-3,2 cm e 1,9- 2,5 cm), as subparcelas e as formas de colheita (colheita total da haste no ponto de colheita comercial da flor; colheita da haste no ponto comercial, mantendo 10 cm da haste no solo; retirada do botão floral assim que seu surgimento foi observado e colheita no fim do ciclo vegetativo), as sub subparcelas. Os bulbos foram plantados em canteiros, em espaçamento de 15 x 20 cm. Foram avaliados o número de plantas que floresceram e número de flores, comprimento e diâmetro do botão floral, massas fresca e seca, diâmetro e altura da planta e número, perímetro e massa fresca e seca de bulbos. Houve decréscimo na altura da
RESUMO
Exposure of bulbs to cold, a physiological phenomenon called vernalization, and bulb size are important factors in the production of lily bulbs and flowers. This study aimed to verify the influence of vernalization of bulbs on flowering cut lily plants, as well as the impact of size and shape of harvest on the production and quality of flowers and bulbs. In turn, the way the stems of the plants used for cut-flower production are cropped is of higher importance for the production of new flower bulbs. In this sense, the experiment was conducted in Viçosa, MG, in a greenhouse in a randomized block design, in split splot scheme with three replications, in which the vernalization periods (25, 35 and 45 days at 4 ± 1 C) constituted the plots; bulb sizes (diameters of 3.2-3.8 cm; 2.5-3.2 cm 1.9- and 2.5 cm), subplots and ways to harvest (full harvest of the stem at the required length for the commercial harvest of the flower, commercial stem harvest at the commercial length, maintaining 10cm of stem in the soil; removal of the floral buds as soon as their appearance is observed and harvest at the end of the season), the sub subplots. The bulbs were planted in beds, with 15 x 20 cm spacing. It was evaluated the number of plants that flowered and the number of flowers, the length and the diameter of the floral buds, fresh and dry weights, diameter and plant height as well as number, per
A exposição dos bulbos ao frio, fenômeno fisiológico denominado vernalização, e o tamanho do bulbo são importantes na produção de flores de lírio. Por sua vez, a forma de colheita das hastes das plantas utilizadas para corte de flor é preponderante na produção de bulbos filhos. Este estudo teve como objetivo verificar a influência da vernalização dos bulbos em plantas de lírio em flor, bem como o impacto do tamanho e forma da colheita na produção e qualidade de flores e bulbos. O experimento foi conduzido em Viçosa, MG casa de vegetação, em blocos casualizados, com parcelas sub-subdivididas, com três repetições, em que os períodos de vernalização (25, 35 e 45 dias, a 4± 1? C) constituíram as parcelas; os tamanhos de bulbo (diâmetros de 3,2- 3,8 cm; 2,5-3,2 cm e 1,9- 2,5 cm), as subparcelas e as formas de colheita (colheita total da haste no ponto de colheita comercial da flor; colheita da haste no ponto comercial, mantendo 10 cm da haste no solo; retirada do botão floral assim que seu surgimento foi observado e colheita no fim do ciclo vegetativo), as sub subparcelas. Os bulbos foram plantados em canteiros, em espaçamento de 15 x 20 cm. Foram avaliados o número de plantas que floresceram e número de flores, comprimento e diâmetro do botão floral, massas fresca e seca, diâmetro e altura da planta e número, perímetro e massa fresca e seca de bulbos. Houve decréscimo na altura da
RESUMO
La intoxicación de los felinos puede ser accidental o intencional. Muchas sustâncias pueden causar síntomas similares, sin embargo, la literatura sobre el tema es escassa y el tratamiento para cada cuadro clínico es muy diferente. En este contexto, este estudio tuvo como objetivo determinar las lesiones patológicas de las intoxicaciones en los gatos en el área cubierta por el Laboratorio Regional de Diagnóstico de la Facultad de Veterinaria de la Universidad Federal de Pelotas, mediante la revisión de los archivos de laboratorio y necropsias realizadas. Se estudiaron 24 casos de intoxicación. La historia clínica fue fundamental para establecer el diagnóstico, clínica y signos y lesiones características. Entre las sustancias involucradas estabanlos derivados de lacumarina, la estricnina, etilenglicol, fluoroacetato sódio, goma de moqueta, antipulgas y Lilium sp.
Poisoning in cats can be accidental or intentional. Many substances can cause similar symptoms. And the literature on the subject are scarce. However, the treatment for each clinical features is very different and yet, the literature on the subject are scarce. This study aimed to determine the pathologicallesions of poisoning in cats in the area covered by the Regional Diagnostic Laboratory of the Veterinary School of the Federal University of Pelotas, by reviewing laboratory files and necropsies. 24 cases of poisoning were studied. The clinical history was fundamental to establish the diagnose, clinical signs and characteristic lesions. The substances involved were coumarin derivatives, strychnine, ethylene glycol, sodium fluoroacetate, carpet glue, anti-fleas and Lilium sp.
As intoxicações em felinos podem ser acidentais ou intencionais. Muitas substâncias podem provocar sintomas semelhantes, e os dados da literatura sobre o assunto são escassos. No entanto o tratamento indicado para cada quadro clínico é diferente. Neste contexto este trabalho teve como objetivo determinar as lesões anatomopatológicas das intoxicações em gatos na área de abrangência do Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal Pelotas, através da revisão de arquivos do laboratório e necropsias realizadas. Foram estudados 24 casos de intoxicação. A história clínica foi fundamental para o estabelecimento do diagnóstico, além de sinais clínicos e lesões características. As substâncias implicadas foram derivadoscumarínicos, estricnina, etilenoglicol, fluoracetato, cola de carpete, antipulgas e Lilium sp.
Assuntos
Animais , Felidae , Intoxicação/diagnóstico , Intoxicação/veterinária , Estricnina/intoxicação , Lilium/intoxicaçãoRESUMO
A reliable somatic embryogenesis protocol is a prerequisite for application of other plant biotechniques. Several protocols were reported for genus Lilium, with variable success. Between them, transverse Thin Cell Layers (tTCL) were used efficiently to induce indirect somatic embryogenesis of Lilium. Somatic embryogenesis potential is dependent on the genotype, explant, and culture medium composition, especially as for plant growth regulators and environmental conditions. Usually, the process comprises three phases: embryogenic callus induction, embryogenic callus proliferation and somatic embryo germination. Somatic embryo germination can be achieved in light or dark. In the first case, complete plantlets are formed, with green leaves and pseudobulb in the base. In darkness, microbulbs are formed from single somatic embryos or clusters. A last phase of microbulb enlargement allows plantlets or microbulbs to increase their biomass. These enlarged microbulbs do not need special acclimatization conditions when transferred to soil and quickly produce sturdy plants. This chapter describes a protocol for somatic embryogenesis of Lilium using tTCL from microbulbs.
Assuntos
Lilium/crescimento & desenvolvimento , Raízes de Plantas/crescimento & desenvolvimento , Técnicas de Embriogênese Somática de Plantas/métodos , Técnicas de Cultura de Tecidos/métodos , Aclimatação/genética , Germinação/genética , Lilium/genética , Desenvolvimento Vegetal/genética , Reguladores de Crescimento de Plantas/metabolismo , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Raízes de Plantas/genética , Brotos de Planta/genética , Brotos de Planta/crescimento & desenvolvimento , Plantas Geneticamente Modificadas/genéticaRESUMO
Fluctuating asymmetry is a biological phenomenon defined by directionally departures from perfect symmetry and is hypothesized to indicate the inability of an organism to maintain precise development. Furthermore, its measurement permits evaluation of levels of stress during development of organisms, since asymmetric patterns indicate a stressful environment during ontogeny. This study aimed to assess whether there is significant difference between body sides in two bat species adapted to urban environments and if there is significant difference in levels of asymmetry in various body regions. Bats, Artibeus planirostris (n = 89) and Sturnira lilium (n = 36), were studied. In most body parts, there was no significant difference between sides, suggesting that species adapted to a human environment were not hampered. Moreover, we observed that bat forelimbs had the lowest levels of asymmetry (p 0.05 between the hindlimb and forelimb, and p 0.01 between the head and forelimb, on females of S. lilium). Some studies suggest a ranking of importance of body parts based on the degree of asymmetry. This study highlighted the importance of the forelimbs in bats and suggested that they are not hampered by adaptation to human environments.(AU)
Assuntos
Animais , Quirópteros/anatomia & histologia , Quirópteros/crescimento & desenvolvimento , Adaptação Biológica , Adaptação a Desastres , Área UrbanaRESUMO
Fluctuating asymmetry is a biological phenomenon defined by directionally departures from perfect symmetry and is hypothesized to indicate the inability of an organism to maintain precise development. Furthermore, its measurement permits evaluation of levels of stress during development of organisms, since asymmetric patterns indicate a stressful environment during ontogeny. This study aimed to assess whether there is significant difference between body sides in two bat species adapted to urban environments and if there is significant difference in levels of asymmetry in various body regions. Bats, Artibeus planirostris (n = 89) and Sturnira lilium (n = 36), were studied. In most body parts, there was no significant difference between sides, suggesting that species adapted to a human environment were not hampered. Moreover, we observed that bat forelimbs had the lowest levels of asymmetry (p 0.05 between the hindlimb and forelimb, and p 0.01 between the head and forelimb, on females of S. lilium). Some studies suggest a ranking of importance of body parts based on the degree of asymmetry. This study highlighted the importance of the forelimbs in bats and suggested that they are not hampered by adaptation to human environments.
Assuntos
Animais , Adaptação Biológica , Adaptação a Desastres , Quirópteros/anatomia & histologia , Quirópteros/crescimento & desenvolvimento , Área UrbanaRESUMO
Water-soluble nutrients are absorbed by the small intestine via transcellular and paracellular mechanisms. Based on a few previous studies, the capacity for paracellular nutrient absorption seems greater in flying mammals than in nonflying mammals, but there has been little investigation of the mechanisms driving this difference. Therefore, we studied three species each of bats (Artibeus lituratus, Sturnira lilium and Carollia perspicillata) and nonflying mammals (Akodon montensis, Mus musculus and Rattus norvegicus). Using standard pharmacokinetic techniques in intact animals, we confirmed the greater paracellular nutrient absorption in the fliers, comparing one species in each group. Then we conducted in situ intestinal perfusions on individuals of all species. In both approaches, we measured the absorption of 3OMD-glucose, a nonmetabolizable glucose analog absorbed both paracellularly and transcellularly, as well as L-arabinose, which has no mediated transport. Fractional absorption of L-arabinose was three times higher in the bat (S. lilium: 1.2±0.24) than in the rodent (A. montensis: 0.35±0.04), whereas fractional absorption of 3OMD-glucose was complete in both species (1.46±0.4 and 0.97±0.12, respectively). In agreement, bats exhibited two to 12 times higher l-arabinose clearance per square centimeter nominal surface area than rodents in intestinal perfusions. Using L-arabinose, we estimated that the contribution of the paracellular pathway to total glucose absorption was higher in all three bats (109-137%) than in the rodents (13-39%). These findings contribute to an emerging picture that reliance on the paracellular pathway for nutrient absorption is much greater in bats relative to nonflying mammals and that this difference is driven by differences in intestinal permeability to nutrient-sized molecules.
Assuntos
Metabolismo dos Carboidratos/fisiologia , Quirópteros/fisiologia , Absorção Intestinal/fisiologia , Intestinos/fisiologia , Animais , Camundongos , Ratos , Sigmodontinae/fisiologia , Especificidade da EspécieRESUMO
Morphology of gametes is used to understand the physiological processes in reproduction among domestic and wild animals. These gametes are used in assisted reproductive technology (ART) and conservation programs. In the case of Artibeus jamaicensis and Sturnira lilium, few studies have been conducted related to these issues. The aim of this study was to describe the structure of spermatozoa, semen characteristics and also the morphology and quality of cumulus oocyte complexes (COC) of A. jamaicensis and S. lilium. Semen characteristics were: A. jamaicensis had a sperm concentration of 4.26×10(6)sperm/ml, progressive motility of 34.55%; viability of 73.23%; head, tail and mid-piece abnormalities of 12.50%. Head length was 6.26µm, mid-piece 18.61µm and tail 70.92µm. S. lilium, had a sperm concentration of 5.15×10(6)sperm/ml, progressive motility of 60.00%, viability of 83.82%; abnormalities in head, tail and mid-piece of 13.77%. Head length was 7.01µm, mid-piece 20.33µm and tail 70.50µm. On average 12.8 of right ovarian oocytes and 9.9 of left ovarian oocytes of A. jamaicensis were recovered. For S. lilium on average 10.7 oocytes from the right ovary and 10.9 oocytes from the left ovary were recovered, ranging in quality from excellent to poor. Sperm morphology and quality of COC were similar to those for other domestic and wild animals. Bat gametes can be used for the study of reproductive biology, in conservation programs and assisted reproductive technology (ART) among domestic and wild animals.
Assuntos
Quirópteros/fisiologia , Oócitos/citologia , Oócitos/fisiologia , Espermatozoides/citologia , Espermatozoides/fisiologia , Animais , Feminino , MasculinoRESUMO
El leucismo consiste en la pérdida completa de un pigmento en particular o de todos los pigmentos, excepto aquellos de partes blandas. Durante un estudio realizado en la selva baja del departamento de Madre de Dios (Perú) se capturó un ejemplar de Sturnira lilium, con pigmentaciones anormales. La coloración general del espécimen es blanca, ojos negros y manchas grises irregulares en las membranas alares. Este es el primer caso de leucismo en un murciélago para Perú y es el primer registro de esta condición para esta especie.
The leucism it consists in the loss of a pigment in particular or all pigments, except those of soft parts. During a study in the low forest of Madre de Dios Department (Peru), we captured a specimen of Sturnira lilium with abnormal pigmentations. The general coloration of the specimen is white, with black eyes and with irregular gray spots on the alar membranes. This is the first case of leucism in a bat from Peru and the first record of this condition for this species.
RESUMO
El leucismo consiste en la pérdida completa de un pigmento en particular o de todos los pigmentos, excepto aquellos de partes blandas. Durante un estudio realizado en la selva baja del departamento de Madre de Dios (Perú) se capturó un ejemplar de Sturnira lilium, con pigmentaciones anormales. La coloración general del espécimen es blanca, ojos negros y manchas grises irregulares en las membranas alares. Este es el primer caso de leucismo en un murciélago para Perú y es el primer registro de esta condición para esta especie.
The leucism it consists in the loss of a pigment in particular or all pigments, except those of soft parts. During a study in the low forest of Madre de Dios Department (Peru), we captured a specimen of Sturnira lilium with abnormal pigmentations. The general coloration of the specimen is white, with black eyes and with irregular gray spots on the alar membranes. This is the first case of leucism in a bat from Peru and the first record of this condition for this species.
RESUMO
Sturnira lilium was colected from December 2006 to May 2009, in remainder of restinga vegetation in Santa Catarina state. In three opportunities we observed the presence of seeds of Billbergia zebrina in feces of S. lilium. The fruits of Bromeliaceae are rare as part of the diet of bats in the Neotropical area. This is the first record of B. zebrine in the diet of S. lilium.
Durante as amostragens de morcegos que ocorreram de dezembro de 2006 a maio de 2009, em remanescente de vegetação de restinga sul de Santa Catarina, em três oportunidades foi observada a presença de sementes de Billbergia zebrina em fezes de indivíduos de Sturnira lilium. O registro de utilização de frutos de bromeliáceas como parte da dieta de morcegos frugívoros na região Neotropical é raro. Este é o primeiro registro de consumo de frutos B. zebrina por S. lilium.
RESUMO
Sturnira lilium was colected from December 2006 to May 2009, in remainder of restinga vegetation in Santa Catarina state. In three opportunities we observed the presence of seeds of Billbergia zebrina in feces of S. lilium. The fruits of Bromeliaceae are rare as part of the diet of bats in the Neotropical area. This is the first record of B. zebrine in the diet of S. lilium.
Durante as amostragens de morcegos que ocorreram de dezembro de 2006 a maio de 2009, em remanescente de vegetação de restinga sul de Santa Catarina, em três oportunidades foi observada a presença de sementes de Billbergia zebrina em fezes de indivíduos de Sturnira lilium. O registro de utilização de frutos de bromeliáceas como parte da dieta de morcegos frugívoros na região Neotropical é raro. Este é o primeiro registro de consumo de frutos B. zebrina por S. lilium.
RESUMO
Flower senescence is triggered by physiological changes including degradation of starch and chlorophyll, increase in respiration and ethylene production. Pulsing of cut flowers with STS is one efficient way to inhibit ethylene action and production. To evaluate the effects of STS combined or not with sucrose on flower longevity and quality, lily inflorescences cv. Ace were pulsed with 5% sucrose for 12 hours, 1mM STS for 15 minutes, STS + sucrose and control (distilled water) in inflorescences harvested with three bud flowers at different ages. They were evaluated at length and opening of the buds, relative water content, content of chlorophyll and longevity. The longevity of lilly flowers variety Ace is influenced by the stage of harvest, with longer longevity for the younger stage, A. Inflorescences harvested at stage A and pulsed with STS had bigger diameter, but the treatment reduced the bud opening and flower quality. The sucrose improved the opening of buds harvested at stage A and increased the flower vase life. Thus the use of sucrose at 5% allowed harvesting the inflorescences at earlier stage, increasing the flower vase life.
A senescência é provocada por mudanças fisiológicas e bioquímicas como degradação do amido e clorofila, aumento da respiração e produção de etileno. O tratamento de flores cortadas com STS constitui uma das formas de inibir a produção ou ação do fitormônio. Para avaliar os efeitos do STS, em combinação ou não com sacarose, aplicado em solução de condicionamento, sobre a longevidade e a qualidade de inflorescências cortadas de lírio, variedade Ace, hastes com 3 botões de diferentes idades, foram tratadas com solução de sacarose 5% por 12 horas; solução de STS 1mM por 15 minutos; solução de STS (15 minutos) + solução de sacarose por 12 horas e controle (água destilada). Foram avaliados: comprimento e abertura dos botões, teor relativo de água e teor de clorofila na folha e longevidade. A longevidade das inflorescências de lírio, variedade Ace, é influenciada pelos estádios de colheita, obtendo-se maior longevidade no estádio mais jovem, A. Inflorescências colhidas no estádio A apresentam maior diâmetro quando pré-tratadas com STS; todavia, este tratamento compromete a abertura dos botões e a qualidade das flores. A sacarose possibilita a abertura dos botões colhidos no estádio A e aumento da vida útil da flor. Assim, o uso de solução de sacarose, na concentração de 5% permite a colheita de inflorecências de lírio em estádio prematuro, obtendo-se maior vida de vaso da flor cortada.
RESUMO
Flower senescence is triggered by physiological changes including degradation of starch and chlorophyll, increase in respiration and ethylene production. Pulsing of cut flowers with STS is one efficient way to inhibit ethylene action and production. To evaluate the effects of STS combined or not with sucrose on flower longevity and quality, lily inflorescences cv. Ace were pulsed with 5% sucrose for 12 hours, 1mM STS for 15 minutes, STS + sucrose and control (distilled water) in inflorescences harvested with three bud flowers at different ages. They were evaluated at length and opening of the buds, relative water content, content of chlorophyll and longevity. The longevity of lilly flowers variety Ace is influenced by the stage of harvest, with longer longevity for the younger stage, A. Inflorescences harvested at stage A and pulsed with STS had bigger diameter, but the treatment reduced the bud opening and flower quality. The sucrose improved the opening of buds harvested at stage A and increased the flower vase life. Thus the use of sucrose at 5% allowed harvesting the inflorescences at earlier stage, increasing the flower vase life.
A senescência é provocada por mudanças fisiológicas e bioquímicas como degradação do amido e clorofila, aumento da respiração e produção de etileno. O tratamento de flores cortadas com STS constitui uma das formas de inibir a produção ou ação do fitormônio. Para avaliar os efeitos do STS, em combinação ou não com sacarose, aplicado em solução de condicionamento, sobre a longevidade e a qualidade de inflorescências cortadas de lírio, variedade Ace, hastes com 3 botões de diferentes idades, foram tratadas com solução de sacarose 5% por 12 horas; solução de STS 1mM por 15 minutos; solução de STS (15 minutos) + solução de sacarose por 12 horas e controle (água destilada). Foram avaliados: comprimento e abertura dos botões, teor relativo de água e teor de clorofila na folha e longevidade. A longevidade das inflorescências de lírio, variedade Ace, é influenciada pelos estádios de colheita, obtendo-se maior longevidade no estádio mais jovem, A. Inflorescências colhidas no estádio A apresentam maior diâmetro quando pré-tratadas com STS; todavia, este tratamento compromete a abertura dos botões e a qualidade das flores. A sacarose possibilita a abertura dos botões colhidos no estádio A e aumento da vida útil da flor. Assim, o uso de solução de sacarose, na concentração de 5% permite a colheita de inflorecências de lírio em estádio prematuro, obtendo-se maior vida de vaso da flor cortada.
RESUMO
In order to evaluate the response of effective vernalization days on the soluble sugar and total nitrogen contents and the emergence of Lilium longiflorum plant shoots, cv Snow Queen bulbs were kept under different vernalization temperatures and periods of treatment under vernalizing temperatures, and cultivated in a plastic greenhouse after the vernalization. The experiment was carried out at the Universidade Federal de Santa Maria, RS, in two seasons: from 03/20/02 to 11/05/02 and from 12/05/02 to 05/15/03. The treatments were 0, 9, 11, 14, 19, 23, 28, 29, 34, 38, 42, 45 and 56 effective vernalization days, calculated with a beta response function to temperature. The soluble sugar and total nitrogen contents, and the duration of the planting-emergence (P-M) phase were determined. The vernalization promoted the mobilization of sugar and nitrogen reserves, which is important for the nutrition of the emergence of the shoot. The increasing number of the effective vernalization days reduced the duration of the P-E phase. Bulbs treated under temperatures of 10ºC for 6 to 8 weeks of storage (34 and 45DV), had the shortest duration of the P-E phase.
Com o objetivo de avaliar a resposta dos dias efetivos de vernalização do lírio sobre o conteúdo de açúcares solúveis e nitrogênio total e a emergência de plantas, bulbos de Lilium longiflorum, cv Snow Queen foram submetidos a diferentes temperaturas de vernalização e períodos de exposição à temperaturas vernalizantes e cultivados em estufa plástica. O experimento foi conduzido na Universidade Federal de Santa Maria, Santa Maria (RS), em duas épocas de plantio dos bulbos: de 20/03/02 a 05/11/02 e 05/12/02 a 15/05/03. Os tratamentos foram 0, 9, 11, 14, 19, 23, 28, 29, 34, 38, 42, 45 e 56 dias efetivos de vernalização (DV), calculados a partir de uma função beta de resposta da vernalização à temperatura. Determinaram-se o conteúdo de açúcares solúveis e nitrogênio total e a duração do subperíodo de plantio dos bulbos-emergência das plantas (P-E). A vernalização promoveu a mobilização de reservas de açúcares e nitrogênio, o que é importante para nutrição da planta em emergência. O aumento do número de dias efetivos de vernalização reduziu a duração do subperíodo P-E. Bulbos tratados em temperaturas de 10ºC por 6 e 8 semanas de armazenamento (34 e 45DV), apresentaram uma menor duração do subperíodo P-E.
RESUMO
In order to evaluate the response of effective vernalization days on the soluble sugar and total nitrogen contents and the emergence of Lilium longiflorum plant shoots, cv Snow Queen bulbs were kept under different vernalization temperatures and periods of treatment under vernalizing temperatures, and cultivated in a plastic greenhouse after the vernalization. The experiment was carried out at the Universidade Federal de Santa Maria, RS, in two seasons: from 03/20/02 to 11/05/02 and from 12/05/02 to 05/15/03. The treatments were 0, 9, 11, 14, 19, 23, 28, 29, 34, 38, 42, 45 and 56 effective vernalization days, calculated with a beta response function to temperature. The soluble sugar and total nitrogen contents, and the duration of the planting-emergence (P-M) phase were determined. The vernalization promoted the mobilization of sugar and nitrogen reserves, which is important for the nutrition of the emergence of the shoot. The increasing number of the effective vernalization days reduced the duration of the P-E phase. Bulbs treated under temperatures of 10ºC for 6 to 8 weeks of storage (34 and 45DV), had the shortest duration of the P-E phase.
Com o objetivo de avaliar a resposta dos dias efetivos de vernalização do lírio sobre o conteúdo de açúcares solúveis e nitrogênio total e a emergência de plantas, bulbos de Lilium longiflorum, cv Snow Queen foram submetidos a diferentes temperaturas de vernalização e períodos de exposição à temperaturas vernalizantes e cultivados em estufa plástica. O experimento foi conduzido na Universidade Federal de Santa Maria, Santa Maria (RS), em duas épocas de plantio dos bulbos: de 20/03/02 a 05/11/02 e 05/12/02 a 15/05/03. Os tratamentos foram 0, 9, 11, 14, 19, 23, 28, 29, 34, 38, 42, 45 e 56 dias efetivos de vernalização (DV), calculados a partir de uma função beta de resposta da vernalização à temperatura. Determinaram-se o conteúdo de açúcares solúveis e nitrogênio total e a duração do subperíodo de plantio dos bulbos-emergência das plantas (P-E). A vernalização promoveu a mobilização de reservas de açúcares e nitrogênio, o que é importante para nutrição da planta em emergência. O aumento do número de dias efetivos de vernalização reduziu a duração do subperíodo P-E. Bulbos tratados em temperaturas de 10ºC por 6 e 8 semanas de armazenamento (34 e 45DV), apresentaram uma menor duração do subperíodo P-E.