RESUMO
The present study investigated the effects of different concentrations of linear crotamine (CrL), used asagent for cell transfection, on in vitro development of bovine embryos. Embryos were exposed to 0; 5 and 12.5µM of CrL in Synthetic Oviductal Fluid (SOF) for 6 h. Vehicle solution was NaCl 150 mM and not exposedembryos (in vitro fertilized-IVF group) was included. In vitro developmental competence was evaluated bycleavage (day 2), blastocyst (day 7 and 8), and hatching (day 8) rates. No difference (P > 0.05) was observedamong CrL groups and control group (IVF) for all observed parameters. In conclusion, the use of the tested CrLconcentrations for 6 h during in vitro culture of bovine embryos have no deleterious effects on embryodevelopment.(AU)
Assuntos
Animais , Feminino , Bovinos , Bovinos/embriologia , Embrião de Mamíferos , Teratogênicos , Peptídeos Penetradores de CélulasRESUMO
The present study investigated the effects of different concentrations of linear crotamine (CrL), used asagent for cell transfection, on in vitro development of bovine embryos. Embryos were exposed to 0; 5 and 12.5µM of CrL in Synthetic Oviductal Fluid (SOF) for 6 h. Vehicle solution was NaCl 150 mM and not exposedembryos (in vitro fertilized-IVF group) was included. In vitro developmental competence was evaluated bycleavage (day 2), blastocyst (day 7 and 8), and hatching (day 8) rates. No difference (P > 0.05) was observedamong CrL groups and control group (IVF) for all observed parameters. In conclusion, the use of the tested CrLconcentrations for 6 h during in vitro culture of bovine embryos have no deleterious effects on embryodevelopment.