A preliminary study of bipolar disorder type I by mass spectrometry-based serum lipidomics.

The present study aimed at investigating possible alterations in the serum lipid profile of euthymic patients with bipolar disorder type I (BD) compared to healthy controls (HC). Thirty-five individuals from both genders were recruited, with 14 diagnosed and treated as BD patients (BD group) and 21 healthy subjects (HC group). Clinical assessment was based on the Structured Clinical Interview for DSM-IV Axis I Disorders (SCID-I), Young Mania Rating Scale (YMRS), and 17-items of Hamilton Depression Rating Scale (HDRS-17) data, which were used to confirm diagnosis, to verify psychiatric comorbidities, and to estimate the severity of manic and depressive symptoms. Ultra-high performance liquid chromatography (UHPLC) coupled to high resolution mass spectrometry (HRMS) was applied to analyze the lipids extracted from all serum samples from both studied groups. In this pioneer and exploratory study, we observed different serum lipid profiles for BD and HC groups, especially regarding glycerophospholipid, glycerolipid, and sphingolipid distribution. Multivariate statistical analyses indicated that 121 lipids were significantly different between BD and HC. Phosphatidylinositols were identified as the most altered lipids in BD patient sera. The results of this preliminary study reinforce the role of lipid abnormalities in BD and offer additional methodological possibilities for investigation in the field.

Serum lipid profile of broilers fed diets with inclusion of mango waste meal / Perfil lipídico sérico de frangos de corte alimentados com inclusão de farelo de resíduo de manga nas rações

We evaluated the effect of including 2.5, 5.0, 7.5 and 10.0% mango waste meal (MWM) variety UBA in corn and soybean meal-based diet son the serum lipid profile of broilers. The experimental design was completely randomized, with five treatments and six replicates with 20 birds per experimental unit. Concentrations of creatinine, albumin, total protein, total cholesterol, triacylglycerols (TAG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and very-low-density lipoprotein cholesterol (VLDL-C), were evaluated at the ages of 14, 28 and 42 days, with 30 birds evaluated per age. At 14 days, there was no difference serum creatinine, total cholesterol, HDL, triacylglycerols, total proteins or VLDL-C concentrations as compared with control. Albumin concentration was the highest at the MWM inclusion levels of 5.0, 7.5 and 10.0%. At 28 days, triacylglycerols, VLDL-C and LDL-C were the lowestat 7.5% inclusion whereas at 42 days, these same variables were the lowest with addition of 10.0% meal. At 14, 28 and 42 days of age, the VLDL-C, LDL-C and total TAG contents were found to decrease at mango waste meal inclusion levels of 5.0 and 7.5% in the diets, which maybe considered an indicator of improvements in the metabolic conditions of broilers.

Avaliou-se o efeito da inclusão de 2,5; 5,0; 7,5 e 10,0% do farelo do resíduo de manga (FRM) variedade Ubá em dietas à base de milho e farelo de soja sobre o perfil lipídico sérico de frangos de corte. O delineamento foi inteiramente casualizado, sendo cinco tratamentos com seis repetições de 20 aves por unidade experimental. Avaliaram-se as concentrações de creatinina, albumina, proteínas totais, colesterol total, triacilglicerois (TAG), lipoproteínas de alta densidade (HDL), lipoproteínas de baixa densidade (LDL), lipoproteínas de densidade de muito baixa (VLDL) nas idades de 14, 28 e 42 dias, sendo avaliadas 30 aves por idade. Aos 14 dias não houve diferença nas concentrações séricas de creatinina, colesterol total, HDL, triacilglicerois, proteínas totais e VLDL quando comparados com o controle. A concentração de albumina foi superior para os níveis de inclusão de 5,0; 7,5 e 10,0% de FRM. Aos 28dias, triacilglicerois, VLDL e LDL foram menores em 7,5% de inclusão, enquanto que aos 42 dias, essas mesmas variáveis foram menores com a adição de 10,0%. Aos 14, 28 e 42dias de idade foram observadas reduções dos teores de VLDL, LDL e TAG totais nos níveis de inclusão de FRM de 5,0% e 7,5% nas rações, isso pode ser considerado como indicador na melhoria das condições metabólicas dos frangos de corte.

Serum lipid profile of broilers fed diets with inclusion of mango waste meal / Perfil lipídico sérico de frangos de corte alimentados com inclusão de farelo de resíduo de manga nas rações

We evaluated the effect of including 2.5, 5.0, 7.5 and 10.0% mango waste meal (MWM) variety UBA in corn and soybean meal-based diet son the serum lipid profile of broilers. The experimental design was completely randomized, with five treatments and six replicates with 20 birds per experimental unit. Concentrations of creatinine, albumin, total protein, total cholesterol, triacylglycerols (TAG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and very-low-density lipoprotein cholesterol (VLDL-C), were evaluated at the ages of 14, 28 and 42 days, with 30 birds evaluated per age. At 14 days, there was no difference serum creatinine, total cholesterol, HDL, triacylglycerols, total proteins or VLDL-C concentrations as compared with control. Albumin concentration was the highest at the MWM inclusion levels of 5.0, 7.5 and 10.0%. At 28 days, triacylglycerols, VLDL-C and LDL-C were the lowestat 7.5% inclusion whereas at 42 days, these same variables were the lowest with addition of 10.0% meal. At 14, 28 and 42 days of age, the VLDL-C, LDL-C and total TAG contents were found to decrease at mango waste meal inclusion levels of 5.0 and 7.5% in the diets, which maybe considered an indicator of improvements in the metabolic conditions of broilers.(AU)

Avaliou-se o efeito da inclusão de 2,5; 5,0; 7,5 e 10,0% do farelo do resíduo de manga (FRM) variedade Ubá em dietas à base de milho e farelo de soja sobre o perfil lipídico sérico de frangos de corte. O delineamento foi inteiramente casualizado, sendo cinco tratamentos com seis repetições de 20 aves por unidade experimental. Avaliaram-se as concentrações de creatinina, albumina, proteínas totais, colesterol total, triacilglicerois (TAG), lipoproteínas de alta densidade (HDL), lipoproteínas de baixa densidade (LDL), lipoproteínas de densidade de muito baixa (VLDL) nas idades de 14, 28 e 42 dias, sendo avaliadas 30 aves por idade. Aos 14 dias não houve diferença nas concentrações séricas de creatinina, colesterol total, HDL, triacilglicerois, proteínas totais e VLDL quando comparados com o controle. A concentração de albumina foi superior para os níveis de inclusão de 5,0; 7,5 e 10,0% de FRM. Aos 28dias, triacilglicerois, VLDL e LDL foram menores em 7,5% de inclusão, enquanto que aos 42 dias, essas mesmas variáveis foram menores com a adição de 10,0%. Aos 14, 28 e 42dias de idade foram observadas reduções dos teores de VLDL, LDL e TAG totais nos níveis de inclusão de FRM de 5,0% e 7,5% nas rações, isso pode ser considerado como indicador na melhoria das condições metabólicas dos frangos de corte.(AU)