Chasing the objective upper eyelid symmetry formula; R2, RMSE, POC, MAE, and MSE.

PURPOSE: Investigate the most appropriate mathematical formula to objectively express upper eyelid contour symmetry. METHODS: 62 eyes of 31 patients were included in the study. The upper eyelid contour symmetry of the patients was classified subjectively (independent of MRD1) as poor, acceptable, and good by three oculoplastic specialists (senior, expert, and junior surgeon). Bézier curves of the upper lid contour were drawn with ImageJ software (NIH, Bethesda, MA, USA). Using the algorithms created by Author SKC in Spyder (Python 3.7.9.), the symmetry of the Bézier curves of the left eyelids were obtained according to the y-axis, and the mid-pupils of both eyes were superimposed. The lower curve moved vertically to the equal height of the other curve to equalize MRD1's. R2 (Coefficient of determination), RMSE (Root-mean-square error), MSE (Mean squared error), POC (Percentage of co-efficiency), and MAE (Mean absolute error) were calculated. We evaluated the correlation between these objective formulas and the subjective grading of three surgeons using Spearman's rho (ρ). RESULTS: The correlation coefficient of RMSE and MSE were the same for all surgeons grading. There was a strong correlation between the senior surgeon's subjective scoring (N; poor = 8, acceptable = 16, good = 8) and R2, RMSE, POC, MAE (ρ = 0.643, p < 0.001, ρ = -0.607, p < 0.001, ρ = 0.562, p < 0.001, ρ = -0.517, p < 0.001, respectively). We found a strong relationship between the expert surgeon's subjective scoring (N; poor = 9, acceptable = 13, good:10) and R2 (ρ = 0.611, p < 0.001), RMSE (ρ = -0.549, p < 0.001), POC (ρ = 0.511, p < 0.001), and MAE (ρ = -0.450, p < 0.05). We found a strong correlation between junior surgeon's subjective scoring (N; poor = 6, acceptable = 18, good = 8) and R2, RMSE, and POC (ρ: -0.517, p < 0.001; ρ: -0.470, p < 0.001; ρ: 0.521, p < 0.001; respectively) and moderate correlation between MAE (ρ:-0.394, p < 0.05). The highest correlation is observed with R2. CONCLUSIONS: RMSE, MSE, POC, MAE, and especially R2, may quantitatively express upper eyelid contour symmetry, comparable with the oculoplastic surgeon. The highest correlation was observed between the senior surgeon and R2, and decreases with the experience of the surgeon.

Green Method Comparison and Optimization of Anthocyanin Recovery from "Sangiovese" Grape Pomace: A Critical Evaluation of the Design of Experiments Approach.

Grape pomace is the main by-product obtained from wine production that is still enriched in bioactive compounds. Within a framework of waste/by-product reuse through a sustainable approach, various green methods were utilized in this work to recover anthocyanins from the pomace resulting from "Sangiovese" grape vinification. Ultrasound- and Microwave-Assisted Extractions (UAE and MAE) were coupled with the use of green solvents, such as acidified water, an ethanol/water mixture, and Natural Deep Eutectic Solvents (NaDES), and their efficacy was compared with that of a conventional method based on a methanol/acidified water mixture. The Total Anthocyanin Index ranged from 36.9 to 75.2 mg/g DW for UAE, and from 54.4 to 99.6 mg/g DW for MAE, while resulting in 47.1 mg/g DW for conventional extraction. A Design of Experiments (DoE) approach was applied to MAE, the most efficient technique. Temperature, time, and the solid-to-liquid ratio were set as X variables, while malvidin-3-O-glucoside content and antioxidant activity were used as response variables, measured by High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The correlation between temperature and time and the antioxidant activity of the extract was positive, while it was found to be negative when considering malvidin-3-O-glucoside concentration as a response variable. Thus, the optimal conditions in temperature, time and solid-to-liquid ratio were different depending on the chosen variable. The results underline the importance of selecting an accurate response when using the response surface methodology approach.

Inattentional aftereffects: The role of attention on the strength of the motion aftereffect.

The way that attention affects the processing of visual information is one of the most intriguing fields in the study of visual perception. One way to examine this interaction is by studying the way perceptual aftereffects are modulated by attention. In the present study, we have manipulated attention during adaptation to translational motion generated by coherently moving random dots, in order to investigate the effect of the distraction of attention on the strength of the peripheral dynamic motion aftereffect (MAE). A foveal rapid serial visual presentation task (RSVP) of varying difficulty was introduced during the adaptation period while the adaptation and test stimuli were presented peripherally. Furthermore, to examine the interaction between the physical characteristics of the stimulus and attention, we have manipulated the motion coherence level of the adaptation stimuli. Our results suggested that the removal of attention through an irrelevant task modulated the MAE's magnitude moderately and that such an effect depends on the stimulus strength. We also showed that the MAE still persists with subthreshold and unattended stimuli, suggesting that perhaps attention is not required for the complete development of the MAE.

Famílias ouvintes e filhos surdos: o papel da Libras na comunicação / Hearing families and deaf children: the role of Libras in communication / Familias oyentes y niños sordos: el papel de Lengua de Señas Brasileña em la comunicación

Introdução: A Língua Brasileira de Sinais (Libras) é uma língua de natureza visuo-motora com um sistema linguístico e estrutura gramatical próprio e sua aquisição em tempo oportuno é importante para o desenvolvimento cognitivo e comunicativo da criança surda. As famílias ouvintes de crianças surdas devem se engajar no aprendizado dessa língua, uma vez que na ausência do conhecimento da Libras, podem apresentar dificuldades de comunicação e de relacionamento com seu filho surdo. Objetivo: O objetivo deste estudo foi compreender o papel da Libras na comunicação de familiares ouvintes e seus filhos surdos atendidos em um centro de reabilitação. Métodos: Trata-se de um estudo transversal, de caráter qualitativo. Foi aplicado um roteiro de entrevista semiestruturado com 10 questões abertas a pais ouvintes de crianças surdas que participam de atendimentos em um centro de reabilitação. A análise dos dados foi realizada por meio do método análise de conteúdo. Resultados: Ao todo foram entrevistadas 10 mães de crianças surdas. As idades das mães variaram de 21 a 47 anos. Nove mães afirmaram usar a Libras em casa com seus filhos todos os dias e todas relataram usá-la em atividades cotidianas da criança. Todas as mães afirmaram que o uso da Libras trouxe benefícios para o relacionamento na díade mãe-filho. Conclusão: Os achados revelam um importante papel da Libras tanto na comunicação de familiares ouvintes e seus filhos surdos, como no cotidiano desses lares. (AU)

Introduction: The Brazilian Sign Language (Libras) is a visual-motor language with its linguistic system and grammatical structure and its timely acquisition is important for deaf children's cognitive and communicative development. Deaf children's hearing families should engage in learning this language, since not knowing Libras may lead to communication and relationship difficulties with their deaf children. Objective: This study aimed to understand the role of Libras in the communication between hearing family members and their deaf children treated at a rehabilitation center. Methods: This cross-sectional qualitative study applied a semi-structured interview with 10 open-ended questions to hearing parents of deaf children who receive care at a rehabilitation center. Data were analyzed with the content analysis method. Results: Altogether, 10 mothers of deaf children were interviewed. Their ages ranged from 21 to 47 years. Nine mothers said they used Libras at home with their children every day, and all reported using it in their child's daily activities. All mothers stated that the use of Libras brought benefits to the mother-child relationship. Conclusion: The findings highlight the important role of Libras in the daily lives and communication of hearing family members and their deaf children. (AU)

Introducción: La Lengua de Señas Brasileña (Libras) es una lengua visomotora con sistema lingüístico y estructura gramatical propios y su adquisición oportuna es importante para el desarrollo cognitivo y comunicativo del niño sordo. Las familias oyentes de niños sordos deben involucrarse en el aprendizaje de este idioma, ya que en ausencia del conocimiento de Libras, pueden tener dificultades en la comunicación y las relaciones con su hijo sordo. Objetivo: El objetivo de este estudio fue comprender el papel de Libras en la comunicación de los familiares oyentes y sus hijos sordos atendidos en un centro de rehabilitación auditiva. Métodos: Se trata de un estudio transversal, cualitativo. Se aplicó un guión de entrevista semiestructurada con 10 preguntas abiertas a padres oyentes de niños sordos que participan en el cuidado en un centro de rehabilitación. El análisis de los datos se realizó mediante el método de análisis de contenido. Resultados: En total, se entrevistaron 10 madres de niños sordos. Las edades de las madres oscilaron entre 21 y 47 años. Nueve madres dijeron que usan Libras en casa con sus hijos todos los días y todas informaron que lo usan en las actividades diarias de sus hijos. Todas las madres afirmaron que el uso de Libras trajo beneficios a la relación madre-hijo. Conclusión: Los hallazgos revelan un papel importante de Libra tanto en la comunicación de los miembros oyentes de la familia y sus hijos sordos, como en la vida diaria de estos hogares. (AU)

Inhibition Effects and Mechanisms of Marine Compound Mycophenolic Acid Methyl Ester against Influenza A Virus.

Influenza A virus (IAV) can cause infection and illness in a wide range of animals, including humans, poultry, and swine, and cause annual epidemics, resulting in thousands of deaths and millions of hospitalizations all over the world. Thus, there is an urgent need to develop novel anti-IAV drugs with high efficiency and low toxicity. In this study, the anti-IAV activity of a marine-derived compound mycophenolic acid methyl ester (MAE) was intensively investigated both in vitro and in vivo. The results showed that MAE inhibited the replication of different influenza A virus strains in vitro with low cytotoxicity. MAE can mainly block some steps of IAV infection post adsorption. MAE may also inhibit viral replication through activating the cellular Akt-mTOR-S6K pathway. Importantly, oral treatment of MAE can significantly ameliorate pneumonia symptoms and reduce pulmonary viral titers, as well as improving the survival rate of mice, and this was superior to the effect of oseltamivir. In summary, the marine compound MAE possesses anti-IAV effects both in vitro and in vivo, which merits further studies for its development into a novel anti-IAV drug in the future.

Surface Modification of Magnetoactive Elastomers by Laser Micromachining.

It has been recently demonstrated that laser micromachining of magnetoactive elastomers is a very convenient method for fabricating dynamic surface microstructures with magnetically tunable properties, such as wettability and surface reflectivity. In this study, we investigate the impact of the micromachining process on the fabricated material's structural properties and its chemical composition. By employing scanning electron microscopy, we investigate changes in size distribution and spatial arrangement of carbonyl iron microparticles dispersed in the polydimethylsiloxane (PDMS) matrix as a function of laser irradiation. Based on the images obtained by a low vacuum secondary electron detector, we analyze modifications of the surface topography. The results show that most profound modifications occur during the low-exposure (8 J/cm2) treatment of the surface with the laser beam. Our findings provide important insights for developing theoretical models of functional properties of laser-sculptured microstructures from magnetoactive elastomers.

Creencias sobre prácticas de alimentación y obesidad de madres con hijos menores de 5 años del noroeste de México / Crenças sobre práticas de alimentação e obesidade entre mães com filhos menores de 5 anos de idade no noroeste do México / Beliefs about feeding practices and obesity among mothers with children under 5 years of age in northwestern Mexico

Introducción: Existe una tendencia mundial de incrementoen los índices de sobrepeso y obesidad en niños menoresde 5 años. Las creencias, percepciones y actitudes de lasmadres hacia las prácticas de alimentación y la obesidadtienen implicaciones directas sobre la salud del hijo.Objetivo: Explorar creencias sobre prácticas de alimentacióny obesidad infantil entre madres con hijos menores de cincoaños del noroeste de México.Metodología: Estudio cualitativo de aproximación etnográfica.Mediante un muestreo propositivo se realizaron nueve entrevistassemiestructuradas a mujeres que contaban con un promediode 12 años de educación.Resultados: Las creencias incluyeron inseguridad para asumirel rol de madre, una baja percepción de apoyo familiar ycreencias que no se alineaban con las recomendaciones dela lactancia materna exclusiva e introducción de alimentoscomplementarios. Además, estas tenían la creencia de que laobesidad no es un problema debido a la corta edad de sus hijos.Conclusiones: Existe una ambivalencia entre las creencias delas participantes y sus prácticas con relación a la alimentacióny la obesidad infantil. Es imperativo desarrollar estrategiasde educación y comunicación que proporcionen informaciónbasada en evidencia, de fácil acceso y disponible para madrescon la intención de prevenir problemas de nutrición durantela primera infancia.(AU)

Introduction: There is a worldwide trend of increasingrates of overweight and obesity in children under5 years of age. Mothers' beliefs, perceptions, andattitudes towards feeding practices and obesity havedirect implications on their children's health.Aim: To explore beliefs about feeding practices andchildhood obesity among mothers with children underfive years of age in northwestern Mexico.Methodology: Qualitative study with ethnographicapproach. Using purposive sampling, nine semi-structured interviews were conducted with womenwith an average of 12 years of education.Results: Beliefs included insecurity in assuming therole of mother, a low perception of family support, andbeliefs that were not aligned with the recommendations ofexclusive breastfeeding and introduction of complementary foods. In addition, these held the belief that obesity isnot a problem due to the young age of their children.Conclusions: There is ambivalence between theparticipants' beliefs and their practices regarding feedingand childhood obesity. It is imperative to developeducation and communication strategies that provideevidence-based information that is easily accessibleand available to mothers to prevent nutrition problemsduring early childhood.(AU)

Introdução: Há uma tendência mundial de aumentodas taxas de sobrepeso e obesidade em crianças commenos de 5 anos de idade. As crenças, percepções eatitudes das mães com relação às práticas alimentares eà obesidade têm implicações diretas na saúde infantil.Objetivo: explorar as crenças sobre práticas de alimentaçãoe obesidade infantil entre mães de crianças com menosde cinco anos de idade no noroeste do México.Metodologia: estudo qualitativo com uma abordagemetnográfica. Usando amostragem intencional, foramrealizadas nove entrevistas semiestruturadas commulheres com uma média de 12 anos de escolaridade.Resultados: As crenças incluíam insegurança em assumiro papel de mãe, baixa percepção de apoio familiar ecrenças que não se alinhavam com as recomendaçõesde amamentação exclusiva e introdução de alimentoscomplementares. Além disso, elas acreditavam quea obesidade não era um problema devido à poucaidade de seus filhos.Conclusões: Há uma ambivalência entre as crenças epráticas dos participantes com relação à alimentaçãoe à obesidade infantil. É imperativo desenvolverestratégias de educação e comunicação que forneçaminformações baseadas em evidências, facilmenteacessíveis e disponíveis para as mães, a fim de evitarproblemas de nutrição na primeira infância.(AU)

9,9'-epoxylignans from Syringa pinnatifolia: A typical case of stereochemical assignment by a quantum chemical calculation with MAEΔΔδ parameter.

In the current study, twenty-two stereochemical 9,9'-epoxylignans including 19 undescribed ones were isolated from the ethanol extract of Syringa pinnatifolia in our continuing effort to understand the overall chemical spectrum of this species. These isolates were structurally elucidated by extensive spectroscopic data analysis, X-ray diffraction, modified Mosher's method, and quantum chemical calculations. Meanwhile, the utilization of 13C NMR calculation and the MAEΔΔδ parameter facilitated the stereochemical assignment of groups of lignan stereoisomers. The 13C NMR data were corrected by the averaged errors at each corresponding carbon position in groups of lignan stereoisomers, which improved the theoretic 13C NMR calculation. The finding of the stereochemical structures of 9,9'-epoxylignans is significant. It is helpful to determine the absolute configurations of molecules with the similar core. In addition, these lignans exhibited potential cardioprotective activities on H9c2 cardiomyocytes in vitro and presented significant antioxidant effect.

Necrophagous flies as bioindicators in Cd and Zn co-contaminated areas of Tak Province, Thailand.

Necrophagous flies may be effective bioindicators of chemical substances within polluted locations, as they are sensitive to environmental changes, have large populations, and thrive in a single location over their lifespan. Diversity and abundance of necrophagous flies were determined at livestock farms contaminated with potentially toxic elements (PTEs) in Tak Province and Nakhon Sawan Province, Thailand. Substantial soil zinc (Zn) concentrations (> 1100 mg kg-1) were detected at a cattle farm at Khaothong, Nakhon Sawan Province, and soil cadmium (Cd) values were significantly elevated (> 3 mg kg-1) at a cattle farm in Pha De, Tak Province. Anthropogenic inputs including Zn mining, domestic wastewater, and certain materials used in local agriculture were point sources of PTEs at the livestock farms in the Pha De and Khaothong subdistricts. Lower temperatures and humidity during the rainy season may have resulted in increased numbers of necrophagous flies, which was 1.5 times greater compared to the dry season. However, the dry season exhibited a higher PTE buildup in fly tissue. The order of important value index (IVI) values of the necrophagous flies were: Chrysomya megacephala (56.80), Musca domestica (27.21), C. rufifacies (25.40) and Sarcophaga spp. (17.54), respectively. These necrophagous flies may play a significant role in PTE-contaminated ecosystems based on their high IVI values, suggesting that they could be used as bioindicators of PTEs. Principal component analysis (PCA) results for necrophagous flies associated with each sampling site during the dry season were consistent with flies having substantial IVI values. Musca domestica and C. megacephala of both sexes displayed substantial correlations with Cr, Al, and Mn, while females of Sarcophaga spp. displayed strong associations with Cd. At the cattle farm in Khaothong, males of M. domestica showed a significant relationship with Zn, Cu, Pb, and Ni. When considering PTE accumulation capacity in flies commonly found at field sites, C. megacephala and M. domestica are the most suitable bioindicators of PTEs. This study confirms that necrophagous flies serve as reliable bioindicators of PTE pollution.

Fatores que influenciam na interação mãe-filho-ambiente no processo de amamentação: estudo transversal / Factors influencing mother-child-environment interaction in the breastfeeding process: a cross-sectional study / Factores que influyen en la interacción madre-hijo-entorno en el proceso de lactancia materna: un estudio transversal

Objetivo: analisar os fatores que influenciam na interação mãe-filho-ambiente no processo de amamentação. Métodos: trata-se de estudo transversal realizado na Rede de Atenção Básica de Saúde de um município do interior do Maranhão, Brasil, com mães de crianças até um ano de idade em processo de amamentação. A coleta de dados foi realizada na Unidade Básica de Saúde ou domicílio, entre abril e julho de 2021, utilizando a Escala Interativa de Amamentação (EINA). Utilizaram-se testes t de Studente ANOVA a um fator, com p-valor < 0,05. Resultados: a média do total de pontuação na escala foi de 210,75 (Desvio Padrão - DP ±16,95), indicando adequada interação entre mãe-filho-ambiente no processo de amamentação. Ter maior escolaridade (ensino superior completo), ser casada e desejar amamentar apresentaram associação com a interação mãe-filho-ambiente no processo de amamentação. Conclusões: maior escolaridade, ser casada e desejar amamentar estão associadas à maior interação mãe-filho-ambiente no processo de amamentação. Quanto maior a vontade de amamentar, maior será a interação mãe-filho-ambiente no processo de amamentação. Estes achados permitem planejar estratégias para melhorar a identificação de binômios com maiores riscos de desmame precoce; e desenvolver ações específicas para o cuidado frente aos preditores que interferem no processo de amamentação.

Objective: analyze the factors that influence the mother-child-environment interaction in the breastfeeding process. Methods: this is a cross-sectional study carried out in the Primary Health Care Network of a municipality in the interior of Maranhão, Brazil, with mothers of children up to one year old who were breastfeeding. Data was collected at the Basic Health Unit or in their households, between April and July 2021, using the Interactive Breastfeeding Scale (EINA, as per its acronym in Portuguese). Student's t and one-way ANOVA tests were used, with a p-value < 0.05. Results: the average total score on the scale was 210.75 (Standard Deviation - SD ± 16.95), indicating adequate interaction between mother-child-environment in the breastfeeding process. Having a higher level of education (complete higher education), being married and wanting to breastfeed were associated with mother-child-environment interaction in the breastfeeding process. Conclusions: higher levels of education, being married and wanting to breastfeed are associated with greater mother-child interaction in the breastfeeding process. The greater the desire to breastfeed, the greater the mother-child-environment interaction in the breastfeeding process. These findings make it possible to plan strategies to improve the identification of pairs at greater risk of early weaning, and to develop specific actions for caring for the predictors that interfere with the breastfeeding process.

Objetivo: analizar los factores que influyen en la interacción madre-hijo-entorno en el proceso de lactancia materna. Métodos: Se trata de un estudio transversal realizado en la Red de Atención Primaria de Salud de un municipio del interior de Maranhão, Brasil, con madres de niños de hasta un año que estaban amamantando. Los datos fueron recolectados en la Unidad Básica de Salud o en el domicilio, entre abril y julio de 2021, utilizando la Escala Interactiva de Lactancia Materna (EINA, por sus siglas en portugués). Se utilizaron pruebas t de Student y ANOVA unidireccional, con un valor p < 0,05. Resultados: la puntuación media total de la escala fue de 210,75 (desviación estándar - DE ± 16,95), lo que indica una interacción adecuada entre madre-hijo-entorno en el proceso de lactancia. Tener un mayor nivel de educación (educación superior completa), estar casada y querer amamantar se asociaron con la interacción madre-hijo-ambiente en el proceso de lactancia. Conclusiones: mayores niveles de educación, estar casada y querer amamantar se asocian a una mayor interacción madre-hijo-entorno en el proceso de lactancia. Cuanto mayor es el deseo de amamantar, mayor es la interacción madre-hijo-entorno en el proceso de lactancia. Estos hallazgos permiten planificar estrategias para mejorar la identificación de las parejas con mayor riesgo de destete precoz y desarrollar acciones específicas para el cuidado de los predictores que interfieren en el proceso de lactancia.

U-Pb dating for zircons from granitic rocks in southwestern Cambodia.

U-Pb dating was conducted for zircons from a total of 14 samples from 13 granite bodies in southwestern Cambodia using LA-ICP-MS. The granitic rock samples were collected from southwestern Cambodia, southwest of the Mae Ping Fault extending from northwest Cambodia via Tonle Sap Lake to southern Vietnam. The studied rock bodies belong to the ilmenite-series, except for three granitic rock bodies. They were identified as I-or A-type. The analysis yielded three distinct age ranges: 295-309, 191-232, and 75-98 Ma. The 295-309 Ma ages are associated with the Paleo-Tethys Sea subduction beneath the Indochina Block. The ages of 191-232 Ma may correspond to the amalgamation period of the Sibumasu and Indochina Blocks during the Indosinian Orogeny. Granitic rocks with ages of 75-98 Ma occur near the southeastern Cambodia-southern Vietnam border. Formation of these granitic rocks was associated with the Paleo-Pacific Ocean Plate (the Izanagi Plate) subduction beneath the Indochina Block. The region in which these granitic rocks occur is part of the Dalat-Kratie Zone.

Phytochemical and Pharmacological Properties of a Traditional Herb, Strobilanthes Cusia (Nees) Kuntze.

The present investigation aimed to determine the effectiveness of bioactive components extracted from Hom herbs (Strobilanthes cusia (Nees) Kuntze) using the solvent-free microwave-assisted extraction (MAE) method. The obtained bioactive components were analyzed for total phenolic content (TPC) and active ingredient content. The Hom extracts were examined for antioxidant, antibacterial, anti-inflammatory, cytotoxic, and anticancer activities. The comparative analysis of extraction methods MAE was studied by using different solvents such as ethanol (EtOH), 50% ethanol (50EtOH) and distilled water (DW). The results obtained by the MAE method with DW as solvent show the TPC of 104.41±1.36 mg GAE/g crude and tryptanthrin 0.1138±0.0014 mg/g crude and indigo 0.0622±0.0015 mg/g crude. Comparatively, values ​​detected in the 50% EtOH extract were not significantly different at the 95% confidence level. At the same time, levels of indirubin were detected at levels equivalent to that of ethanol extracts. The DW extract from MAE had an IC50 value against the DPPH scavenging assay of 0.1927±0.0756 mg/ml, comparable to the test results of extracts of ethanol and 50% ethanol. The bioactive extracted using the MAE with water as solvent had minimum inhibitory concentration (MIC) and could suppress infection at 10 mg/disc. It was also observed that the extracts from the conventional extraction technique using ethanol as the solvent continued to be highly effective against Bacillus cereus even after employing the EtOH or 50% EtOH. Hom extract's MIC value representing inhibiting B. cereus was 0.625 mg/disc. Still, EtOH-extracted Hom demonstrated the highest cytotoxicity against 16HBEo- by reducing cell survival rate by less than 50% while the others did not. Interestingly, Hom that had been extracted using 50EtOH and DW with MAE had an anticancer impact on A549 by reducing the survival rate in a dose-dependent manner.

Evaluation of predictive capability of Bayesian spatio-temporal models for Covid-19 spread.

BACKGROUND: Bayesian models have been applied throughout the Covid-19 pandemic especially to model time series of case counts or deaths. Fewer examples exist of spatio-temporal modeling, even though the spatial spread of disease is a crucial factor in public health monitoring. The predictive capabilities of infectious disease models is also important. METHODS: In this study, the ability of Bayesian hierarchical models to recover different parts of the variation in disease counts is the focus. It is clear that different measures provide different views of behavior when models are fitted prospectively. Over a series of time horizons one step predictions have been generated and compared for different models (for case counts and death counts). These Bayesian SIR models were fitted using MCMC at 28 time horizons to mimic prospective prediction. A range of goodness of prediction measures were analyzed across the different time horizons. RESULTS: A particularly important result is that the peak intensity of case load is often under-estimated, while random spikes in case load can be mimicked using time dependent random effects. It is also clear that during the early wave of the pandemic simpler model forms are favored, but subsequently lagged spatial dependence models for cases are favored, even if the sophisticated models perform better overall. DISCUSSION: The models fitted mimic the situation where at a given time the history of the process is known but the future must be predicted based on the current evolution which has been observed. Using an overall 'best' model for prediction based on retrospective fitting of the complete pandemic waves is an assumption. However it is also clear that this case count model is well favored over other forms. During the first wave a simpler time series model predicts case counts better for counties than a spatially dependent one. The picture is more varied for morality. CONCLUSIONS: From a predictive point of view it is clear that spatio-temporal models applied to county level Covid-19 data within the US vary in how well they fit over time and also how well they predict future events. At different times, SIR case count models and also mortality models with cumulative counts perform better in terms of prediction. A fundamental result is that predictive capability of models varies over time and using the same model could lead to poor predictive performance. In addition it is clear that models addressing the spatial context for case counts (i.e. with lagged neighborhood terms) and cumulative case counts for mortality data are clearly better at modeling spatio-temporal data which is commonly available for the Covid-19 pandemic in different areas of the globe.

Idealização da maternidade e herança psíquica: reflexões no contemporáneo / Idealization of motherhood and psychic heritage: reflections in the contemporary / Idealización de la maternidad y herencia psíquica: reflexiones en lo contemporáneo

Considerando as transformações na maternidade ao longo da história e a atual crise identitária na relação da mulher com a maternidade, ou seja, o conflito entre os ideais de maternidade relacionados à figura da mulher/mãe e as demandas contemporâneas de realização individual no trabalho, realizou-se este estudo transversal, exploratório-descritivo de abordagem qualitativa, cujo objetivo consistia em descrever e analisar, a partir da psicanálise de casal e família, a experiência de mães que abandonaram da carreira profissional para a dedicação exclusiva à maternidade. Foram entrevistadas oito mulheres de classe média, entre 30 e 50 anos, que eram mães e abandonaram suas carreiras profissionais para se dedicarem à maternidade. As entrevistas que compuseram os resultados foram transcritas e as análises de conteúdo temática apontaram as múltiplas exigências, tanto sociais, quanto relacionadas à herança psíquica, que trazem implicações para a construção identitária dessas mulheres. Concluímos que um processo de elaboração dessa herança exige uma rede vincular de apoio e pressupõe a desidealização da maternidade, entendendo-a como plural, tanto no sentido de ser múltipla e diversa, quanto no sentido de ser uma construção vincular.

Noticing the history transformations in motherhood, and the current identity crisis in the woman's relationship between femininity and maternity, that is, the conflict between the ideas of motherhood related to the figure of the woman/mother and the contemporary demands of individual realization at work, this exploratory-descriptive, cross-sectional, study of a qualitative approach was carried out, whose objective was to describe and analyze, from the perspective of psychoanalysis of the linking configurations, the experience of mothers who abandoned the professional career for the exclusive dedication to motherhood. Eight middle-class women, aged between 30 and 50, who were mothers and abandoned their professional careers to dedicate themselves to motherhood-retirar, were interviewed. The interviews that composed the results were transcribed, and thematic content analyses pointed out the multiple demands, both social and related to psychic heritage, which have implications for the identity construction of these women. We conclude that an elaboration of this heritage requires a link network of support and presupposes the non-idealization of motherhood, understanding it as plural, both in the sense of being multiple and diverse, and in the sense of being a bonding construction.

Considerando los cambios en la maternidad a lo largo de la historia y la actual crisis de identidad en la relación de la mujer con la maternidad, es decir, el conflicto entre los ideales de maternidad relacionados con la figura de la mujer/madre y las demandas contemporáneas de realización individual en el trabajo, se realizó este estudio transversal, exploratorio-descriptivo de abordaje cualitativo, cuyo objetivo consistía en describir y analizar, a partir del psicoanálisis de las configuraciones vincular, la experiencia de madres que abandonaron de la carrera profesional para la dedicación exclusiva a la maternidad. Fueron entrevistadas ocho mujeres de clase media, entre 30 y 50 años. que eran madres y abandonaron sus carreras profesionales para dedicarse a la maternidad-retirar. Las entrevistas que compusieron los resultados fueron transcritas y los análisis de contenido temático señalaron las múltiples exigencias, tanto sociales, como relacionadas a la herencia psíquica, que traen implicaciones para la construcción identitaria de esas mujeres. Concluimos que un proceso de elaboración de esa herencia exige una red vincular de apoyo y presupone la desidealización de la maternidad, entendiéndola como plural, tanto en el sentido de ser múltiple y diversa, como en el sentido de ser una construcción vincular.

Seasonal Monitoring of Volatiles and Antioxidant Activity of Brown Alga Cladostephus spongiosus.

Cladostephus spongiosus was harvested once a month during its growing season (from May to August) from the Adriatic Sea. Algal volatile organic compounds (VOCs) were obtained by headspace solid-phase microextraction (HS-SPME) and hydrodistillation (HD) and analysed by gas chromatography and mass spectrometry (GC-MS). The effects of air drying and growing season on VOCs were determined. Two different extraction methods (ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE)) were used to obtain ethanolic extracts of C. spongiosus. In addition, the seasonal antioxidant potential of the extracts was determined, and non-volatile compounds were identified from the most potent antioxidant extract. Aliphatic compounds (e.g., pentadecane) were predominantly found by HS-SPME/GC-MS. Hydrocarbons were more than twice as abundant in the dry samples (except in May). Aliphatic alcohols (e.g., hexan-1-ol, octan-1-ol, and oct-1-en-3-ol) were present in high percentages and were more abundant in the fresh samples. Hexanal, heptanal, nonanal, and tridecanal were also found. Aliphatic ketones (octan-3-one, 6-methylhept-5-en-2-one, and (E,Z)-octa-3,5-dien-2-one) were more abundant in the fresh samples. Benzene derivatives (e.g., benzyl alcohol and benzaldehyde) were dominant in the fresh samples from May and August. (E)-Verbenol and p-cymen-8-ol were the most abundant in dry samples in May. HD revealed aliphatic compounds (e.g., heptadecane, pentadecanal, (E)-heptadec-8-ene, (Z)-heptadec-3-ene), sesquiterpenes (germacrene D, epi-bicyclosesquiphellandrene, gleenol), diterpenes (phytol, pachydictyol A, (E)-geranyl geraniol, cembra-4,7,11,15-tetraen-3-ol), and others. Among them, terpenes were the most abundant (except for July). Seasonal variations in the antioxidant activity of the ethanolic extracts were evaluated via different assays. MAE extracts showed higher peroxyl radical inhibition activity from 55.1 to 74.2 µM TE (Trolox equivalents). The highest reducing activity (293.8 µM TE) was observed for the May sample. Therefore, the May MAE extract was analysed via high-performance liquid chromatography with high-resolution mass spectrometry and electrospray ionisation (UHPLC-ESI-HRMS). In total, 17 fatty acid derivatives, 9 pigments and derivatives, and 2 steroid derivatives were found. The highest content of pheophorbide a and fucoxanthin, as well as the presence of other pigment derivatives, could be related to the observed antioxidant activity.

Simultaneous microwave-assisted extraction of bioactive compounds from aged garlic.

In this work, the simultaneous extraction of bioactives (organosulfur compounds, such as S-allyl-L-cysteine (SAC), carbohydrates, such as neokestose and neonystose, and total phenolic compounds) from aged garlic has been optimized for the first time to obtain multifunctional extracts for further application as food ingredients. Analytical methods using liquid chromatography coupled to mass spectrometry (HPLC-MS) and by hydrophilic interaction liquid chromatography with evaporative light scattering detection (HILIC-ELSD) were also previously optimized. High sensitivity (limits of detection between 0.013 and 0.77 µg mL-1) and appropriate repeatability (< 12%) and accuracy (> 92%) for the analysis of bioactives were achieved. After selecting water as the extraction solvent and microwave-assisted extraction (MAE) as the most efficient technique, operation conditions were optimized using a Box-Behnken experimental design (60 min; 120 °C; 0.05 g mL-1; 1 cycle) to maximize the content of bioactives from different aged garlic samples. Regarding organosulfur compounds, only SAC (traces-2.32 mg g-1 dry sample) and cycloalliin (1.23-3.01 mg g-1 dry sample) were detected in all samples, while amino acids such as arginine (0.24-3.45 mg g-1 dry sample) and proline (0.43-3.91 mg g-1 dry sample) were, in general, the most abundant. Bioactive carbohydrates (from trisaccharides to nonasaccharides) were only detected in fresh garlic and aged garlic processed under mild conditions, whereas all garlic extracts showed antioxidant activity. The developed MAE methodology is shown as a successful alternative to other procedures for the simultaneous extraction of aged garlic bioactives intended by the food and nutraceutical industries, among others.

Sex- and age-related differences in outcomes of patients with acute myocardial infarction: MINOCA vs. MIOCA.

AIMS: The aim of the study is to evaluate the impact of sex on acute myocardial infarction (AMI) patients' clinical presentation and outcomes, comparing those with non-obstructive and obstructive coronary arteries (MINOCA vs. MIOCA). METHODS AND RESULTS: We enrolled 2455 patients with AMI undergoing coronary angiography from January 2017 to September 2021. Patients were divided according to the type of AMI and sex: male (n = 1593) and female (n = 607) in MIOCA and male (n = 87) and female (n = 168) in MINOCA. Each cohort was further stratified based on age (≤/> 70 years). The primary endpoint (MAE) was a composite of all-cause death, recurrent AMI, and hospitalization for heart failure (HF) at follow-up. Secondary outcomes included all-cause and cardiovascular death, recurrent AMI, HF re-hospitalization, and stroke. MINOCA patients were more likely to be females compared with MIOCA ones (P < 0.001). The median follow-up was 28 (15-41) months. The unadjusted incidence of MAE was significantly higher in females compared with males, both in MINOCA [45 (26.8%) vs. 12 (13.8%); P = 0.018] and MIOCA cohorts [203 (33.4%) vs. 428 (26.9%); P = 0.002]. Age was an independent predictor of MAE in both cohorts. Among MINOCA patients, females ≤70 years old had a higher incidence of MAE [18 (23.7%) vs. 4 (5.9%); P = 0.003] compared with male peers, mainly driven by a higher rate of re-hospitalization for HF (P = 0.045) and recurrence of AMI (P = 0.006). Only in this sub-group of MINOCA patients, female sex was an independent predictor of MAE (hazard ratio = 3.09; 95% confidence interval: 1.02-9.59; P = 0.040). MINOCA females ≤70 years old had worse outcomes than MIOCA female peers. CONCLUSION: MINOCA females ≤70 years old had a significantly higher incidence of MAE, compared with males and MIOCA female peers, likely due to the different pathophysiology of the ischaemic event. TRIAL REGISTRATION: Data were part of the ongoing observational study 'AMIPE: Acute Myocardial Infarction, Prognostic and Therapeutic Evaluation' (ClinicalTrials.gov Identifier: NCT03883711).

Environmental and Socio-Cultural Factors Impacting the Unique Gene Pool Pattern of Mae Hong-Son Chicken.

Understanding the genetic diversity of domestic chicken breeds under the impact of socio-cultural and ecological dynamics is vital for the conservation of natural resources. Mae Hong Son chicken is a local breed of North Thai domestic chicken widely distributed in Mae Hong Son Province, Thailand; however, its genetic characterization, origin, and diversity remain poorly understood. Here, we studied the socio-cultural, environmental, and genetic aspects of the Mae Hong Son chicken breed and investigated its diversity and allelic gene pool. We genotyped 28 microsatellite markers and analyzed mitochondrial D-loop sequencing data to evaluate genetic diversity and assessed spatial habitat suitability using maximum entropy modeling. Sequence diversity analysis revealed a total of 188 genotyped alleles, with overall nucleotide diversity of 0.014 ± 0.007, indicating that the Mae Hong Son chicken population is genetically highly diverse, with 35 (M1-M35) haplotypes clustered into haplogroups A, B, E, and F, mostly in the North ecotype. Allelic gene pool patterns showed a unique DNA fingerprint of the Mae Hong Son chicken, as compared to other breeds and red junglefowl. A genetic introgression of some parts of the gene pool of red junglefowl and other indigenous breeds was identified in the Mae Hong Son chicken, supporting the hypothesis of the origin of the Mae Hong Son chicken. During domestication in the past 200-300 years after the crossing of indigenous chickens and red junglefowl, the Mae Hong Son chicken has adapted to the highland environment and played a significant socio-cultural role in the Northern Thai community. The unique genetic fingerprint of the Mae Hong Son chicken, retaining a high level of genetic variability that includes a dynamic demographic and domestication history, as well as a range of ecological factors, might reshape the adaptation of this breed under selective pressure.

Ceftriaxone-Loaded Polymeric Microneedles, Dressings, and Microfibers for Wound Treatment.

The objective of this study was to create polymeric dressings, microfibers, and microneedles (MN) loaded with ceftriaxone, using PMVA (Poly (Methyl vinyl ether-alt-maleic acid), Kollicoat® 100P, and Kollicoat® Protect as polymers to treat diabetic wounds and accelerate their recovery. These formulations were optimized through a series of experiments and were subsequently subjected to physicochemical tests. The results of the characterization of the dressings, microfibers, and microneedles (PMVA and 100P) were, respectively, a bioadhesion of 281.34, 720, 720, 2487, and 510.5 gf; a post-humectation bioadhesion of 186.34, 831.5, 2380, and 630.5 gf, tear strength of 2200, 1233, 1562, and 385 gf, erythema of 358, 8.4, 227, and 188; transepidermal water loss (TEWL) of 2.6, 4.7, 1.9, and 5.2 g/h·m2; hydration of 76.1, 89.9, 73.5, and 83.5%; pH of 4.85, 5.40, 5.85, and 4.85; and drug release (Peppas kinetics release) of n: 0.53, n: 0.62, n: 0.62, and n: 0.66). In vitro studies were performed on Franz-type diffusion cells and indicated flux of 57.1, 145.4, 718.7, and 2.7 µg/cm2; permeation coefficient (Kp) of 13.2, 19.56, 42, and 0.00015 cm2/h; and time lag (tL) of 6.29, 17.61, 27. 49, and 22.3 h, respectively, in wounded skin. There was no passage of ceftriaxone from dressings and microfibers to healthy skin, but that was not the case for PMVA/100P and Kollicoat® 100P microneedles, which exhibited flux of 194 and 0.4 µg/cm2, Kp of 11.3 and 0.00002 cm2/h, and tL of 5.2 and 9.7 h, respectively. The healing time of the formulations in vivo (tests carried out using diabetic Wistar rats) was under 14 days. In summary, polymeric dressings, microfibers, and microneedles loaded with ceftriaxone were developed. These formulations have the potential to address the challenges associated with chronic wounds, such as diabetic foot, improving the outcomes.

Mães em cárcere: percepções sobre a vivência da maternidade em privação de liberdade / Mothers in prison: perceptions on the experience of motherhood indeprivation of liberty / Madres en la cárcel: percepciones sobre la experiencia de maternidad en privación de libertad

Objetivo: analisar as percepções de mães encarceradas sobre a vivência da maternidade em privação de liberdade. Método: pesquisa exploratória, transversal, qualitativa, desenvolvida com 19 mulheres de uma penitenciária feminina da região Nordeste do Brasil. Utilizou-se como referencial teórico a Teoria do Apego de John Bowlby. A obtenção dos dados ocorreu por meio de questionário sociodemográfico e entrevista semiestruturada; posteriormente, os dados foram submetidos a análise de conteúdo de Bardin. Resultados: as entrevistadas são, na maioria, mulheres entre 23 a 28 anos, solteiras, com média de 2 filhos e com histórico de uso de álcool e/ou outras drogas, presas por tráfico de drogas. Duas categorias foram estabelecidas: O Significado da Maternidade e Vivência da Maternidade em Privação de Liberdade. Conclusão:observou-se o sofrimento do binômio com a separação parental, ocasionando prejuízos para o crescimento e desenvolvimento das crianças. As mães encarceradas estão expostas ao ambiente insalubre e desumanizado, o que acarreta sofrimento psíquico.

Objective: to analyze the condition of motherhood in deprivation of liberty as perceived by mothers in prison. Method: an exploratory, transversal, qualitative research was conducted using John Bowlby's Attachment Theory as a theoretical framework with 19 mothers from a women's penitentiary in northeastern Brazil. Data were collected by means of a sociodemographic questionnaire and semi-structured interviews, and later examined by Bardin's content analysis. Results: most of the participants are single, between 23 and 28 years old, with an average of two children and a history of alcohol consumption and/or other drugs, arrested for drug trafficking. Content analysis formed two categories: The Meaning of Motherhood and Experiencing Motherhood in Deprivation of Liberty. Conclusion: the binomial mother-infant suffered with parental separation, resulting in child growth and development issues. Incarcerated mothers are exposedto an unhealthy and dehumanized environment, leading to psychological distress

Objetivo: analizar las percepciones de madres privadas de libertad sobre la experiencia de la maternidad en privación de libertad. Método: investigación exploratoria, transversal, cualitativa, desarrollada con 19 mujeres de un centro penitenciario de mujeres del Nordeste de Brazil. Se utilizó como marco teórico la teoría del apego de John Bowlby. La recolección de datos se realizó desde un cuestionario sociodemográfico y una entrevista semiestructurada; posteriormente,se aplicó el análisis de contenido de Bardin. Resultados: los datos mostraron mujeres de entre 23 y 28 años de edad, solteras, con promedio de dos hijos y con antecedentes de consumo de alcohol y/u otras drogas, detenidas por tráfico de estupefacientes. Se establecieron dos categorías: El Sentido de la Maternidad y la Experiencia de la Maternidad Privada de Libertad. Conclusión: se observó el sufrimiento del binomio con la separación parental, provocando perjuicios en el crecimiento y desarrollo de los niños. Las madres encarceladas están expuestas a un entorno insalubre y deshumanizado, lo que genera angustia psicológica.