Screening and identification of natural α-glucosidase and α-amylase inhibitors from partridge tea (Mallotus furetianus Muell-Arg) and in silico analysis.

Partridge leaves (Mallotus furetianus Muell-Arg.) have long been consumed as popular folk substitute tea for treating hyperglycemia in China. In this study, the inhibiting effects of partridge tea extracts on α-glucosidase and α-amylase were investigated, and then effect of partridge tea aqueous extracts (PTAEs) on glucose consumption capacity of 3 T3-L1 preadipocytes cells was determined. Results verified that PTAEs showed excellent anti-α-glucosidase and anti-α-amylase effects. In addition, the PTAEs evidently promoted glucose consumption capacity of 3T3L1 preadipocytes cells. To this end, a combined method of affinity ultrafiltration and HPLC-ESI-qTOF-MS/MS was used for rapidly screening and identifying the potential inhibitors in the PTAEs. Catechin, epicatechin, rutin, ferulic acid, and kaempferitrin with high affinity capacity indicated strong inhibiting effect on α-glucosidase and α-amylase. Docking studies revealed the potential interactive mechanisms between these major inhibitors and two digestive enzymes. This research shows that partridge tea is effective in preventing and treating post hyperglycemia.

Chemical constituents from Mallotus furetianus / 中草药

Objective To study the chemical constituents of Mallotus furetianus. Methods The constituents were isolated and purified by silica gel chromatography and HPLC repeatedly, and the structures were identified by spectra analysis and chemical methods. Results Sixteen compounds were isolated from the leaves of M. furetianus, the structures were identified as 3-hydroxy-4,5 (R)-dimethy1-2 (5H)-furanone (1), gallic acid (2), 3,4,8,9,10-pentahydroxydibenzo [b, d] pyran-6-one (3), epicatechin (4), catechin (5), kaempferol-3-O-robinobioside (6), apigenin (7), 2-hydroxysuccinic acid (8), 5-hydroxymethylfuroic acid (9), gallic acid methyl ester (10), caffeic acid (11), tanshinol (12), diolmycin B2 (13), oresbiusin A (14), 3,4-dihydroxyphenyl-β- D-glucopyranoside (15), and (2S)-pyroglutamic acid (16). Conclusion Compounds 7-16 are obtained from the genus of Mallotus for the first time, and compounds 4-16 are obtained from M. furetianus for the first time.