Effect of suckler cow breed type and parity on the development of the cow-calf bond post-partum and calf passive immunity.

BACKGROUND: Development of the cow-calf bond post-partum and passive immunity of calves from spring-calving beef × beef (B×B) and beef × dairy (B×D) cow genotypes was determined using primiparous and multiparous (Experiment 1), and primiparous and second-parity (Experiment 2) animals. In Experiment 1, calves either suckled colostrum naturally ('natural-suckling') (n = 126), or were fed colostrum, using an oesophageal-tube ('artificially-fed') (n = 26), from their dam within 1-h post-partum. In Experiment 2, all calves (n = 60) were artificially-fed colostrum from their dam. Prior to colostrum suckling/feeding, colostrum was sampled for IgG analysis. The cow-calf bond was assessed using CCTV recordings during the first 4-h post-partum. Calves were blood sampled at 48-h post-partum to determine IgG and total protein (TP) concentrations, and zinc sulphate turbidity (ZST) units. RESULTS: There was no difference (P > 0.05) in cow licking behaviours and calf standing and suckling behaviours between the genotypes, except in Experiment 2 where B×D calves had more attempts to suckle before suckling occurred (P ≤ 0.05) compared to B×B calves. In Experiment 1, multiparous cows licked their calves sooner (P ≤ 0.05) and for longer (P < 0.01), and their calves had fewer attempts to stand (P < 0.001), stood for longer (P = 0.05), and had fewer attempts to suckle before suckling occurred (P < 0.001) than primiparous cows; there was no parity effect on cow-calf behaviour in Experiment 2. Colostrum IgG concentrations and measures of calf passive immunity did not differ (P > 0.05) between the genotypes in either Experiment. In Experiment 1, colostrum IgG concentrations were greater (P ≤ 0.05) in multiparous compared to primiparous cows and their calves had superior (P ≤ 0.05) passive immunity; no effect of parity was found in Experiment 2. Passive immunity did not differ (P > 0.05) between suckled and artificially-fed calves in Experiment 1. CONCLUSIONS: Cow genotype had little effect on cow-calf behaviours, but under 'natural-suckling' conditions primiparous cows expressed maternal inexperience and their calves were less vigorous than multiparous cows. Colostrum IgG concentration and calf passive immunity measures were unaffected by genotype, but under 'natural-suckling' conditions calves from primiparous cows had lower passive immunity.

Twinning in wild, endangered lion-tailed macaques Macaca silenus in the Anamalai Hills of the Western Ghats, India.

Many primate species show various behavioural and ecological adaptations to provisioning, one of which is the unusual occurrence of twins. Here, we report observations on two pairs of surviving twins in lion-tailed macaques Macaca silenus in the Anamalai Hills of the Western Ghats, India. The Puthuthottam population of lion-tailed macaques has historically been restricted to a rainforest fragment measuring 92 ha, situated adjacent to human settlements. Over the last 10 years, however, several groups from this population have begun to directly interact with the local human communities, visiting settlements at a rate of 0.52 events/day and exploiting various anthropogenic food resources. We followed and opportunistically collected behavioural ad libitum data on two sets of twins for a year, between March 2019 and March 2020. Both of the mothers were primarily terrestrial, although the mother with the younger set of twins also used the tree canopy and other precarious substrates, such as cables. Although two previous cases of twinning have been reported in this population, one in the late 1990s and one between 2000 and 2002, neither of those sets of twins survived beyond a few weeks, with at least one infant in each pair dying of unknown causes. We discuss, but discount, the possibility that one of the infants in either set of twins was an adoptee. Our observations indicate that some lion-tailed macaque twins can survive under free-ranging conditions if they receive adequate care from their biological mother or another female. Our findings also provide further evidence of increased rates of twinning as a consequence of dietary changes in synanthropic non-human primate populations.

CRF binding protein activity in the hypothalamic paraventricular nucleus is essential for stress adaptations and normal maternal behaviour in lactating rats.

To ensure the unrestricted expression of maternal behaviour peripartum, activity of the corticotropin-releasing factor (CRF) system needs to be minimised. CRF binding protein (CRF-BP) might be crucial for this adaptation, as its primary function is to sequester freely available CRF and urocortin1, thereby dampening CRF receptor (CRF-R) signalling. So far, the role of CRF-BP in the maternal brain has barely been studied, and a potential role in curtailing activation of the stress axis is unknown. We studied gene expression for CRF-BP and both CRF-R within the paraventricular nucleus (PVN) of the hypothalamus. In lactating rats, Crh-bp expression in the parvocellular PVN was significantly higher and Crh-r1 expression in the PVN significantly lower compared to virgin rats. Acute CRF-BP inhibition in the PVN with infusion of CRF(6-33) increased basal plasma corticosterone concentrations under unstressed conditions in dams. Furthermore, while acute intra-PVN infusion of CRF increased corticosterone secretion in virgin rats, it was ineffective in vehicle (VEH)-pre-treated lactating rats, probably due to a buffering effect of CRF-BP. Indeed, pre-treatment with CRF(6-33) reinstated a corticosterone response to CRF in lactating rats, highlighting the critical role of CRF-BP in maintaining attenuated stress reactivity in lactation. To our knowledge, this is the first study linking hypothalamic CRF-BP activity to hypothalamic-pituitary-adrenal axis regulation in lactation. In terms of behaviour, acute CRF-BP inhibition in the PVN under non-stress conditions reduced blanket nursing 60 min and licking/grooming 90 min after infusion compared to VEH-treated rats, while increasing maternal aggression towards an intruder. Lastly, chronic intra-PVN inhibition of CRF-BP strongly reduced maternal aggression, with modest effects on maternal motivation and care. Taken together, intact activity of the CRF-BP in the PVN during the postpartum period is essential for the dampened responsiveness of the stress axis, as well as for the full expression of appropriate maternal behaviour.

Do human-wildlife interactions predict offspring hiding strategies in peri-urban fallow deer?

Human activities can induce significant behavioural changes in wildlife. Often explored through extractive interactions (e.g. hunting) that can favour certain behavioural traits, the implications of non-extractive ones, such as wildlife feeding, remain understudied. Research shows that people tend to favour bolder individuals within populations despite their dynamics and consequences being unclear. Using fallow deer in a peri-urban environment, we studied whether mothers that show reduced fear of humans and consistently approach them for food adopt weaker anti-predator strategies by selecting less concealed fawning bedsites closer to human hotspots. This would provide the advantage of additional feeding opportunities in comparison with shyer mothers while keeping their fawns close. Our dataset encompassed 281 capture events of 172 fawns from 110 mothers across 4 years. Surprisingly, mothers that regularly accepted food from humans selected more concealed bedsites farther from human hotspots, giving their offspring better protection while also benefitting from additional food during lactation. Our results show behavioural adaptations by a subset of females and, for the first time, link the tendency to approach humans and strategies to protect offspring. Given previous findings that these begging females also deliver heavier fawns at birth, our research further investigates human-wildlife feeding interactions and their behavioural implications.

High fat diet-induced maternal obesity in mice impairs peripartum maternal behaviour.

Obesity during pregnancy represents a significant health issue and can lead to increased complications during pregnancy and impairments with breastfeeding, along with long-term negative health consequences for both mother and offspring. In rodent models, diet-induced obesity (DIO) during pregnancy leads to poor outcomes for offspring. Using a DIO mouse model, consisting of feeding mice a high fat diet for 8 weeks before mating, we recapitulate the effect of high pup mortality within the first 3 days postpartum. To examine the activity of the dam around the time of birth, late pregnant control and DIO dams were recorded in their home cages and the behaviour of the dam immediately before and after birth was analysed. Prior to giving birth, DIO dams spent less time engaging in nesting behaviour, while after birth, DIO dams spent less time in the nest with their pups compared to control dams, indicating reduced pup-engagement in the early postpartum period. We have previously reported that lactogenic hormone action, mediated by the prolactin receptor, in the medial preoptic area of the hypothalamus (MPOA) is critical for the onset of normal postpartum maternal behaviour. We hypothesized that DIO dams may have lower lactogenic hormone activity during late pregnancy, which would contribute to impaired onset of normal postpartum maternal behaviour. Day 16 lactogenic activity, transport of prolactin into the brain, and plasma prolactin concentrations around birth were all similar in control and DIO dams. Moreover, endogenous pSTAT5, a marker of prolactin receptor activity, in the MPOA was unaffected by DIO. Overall, these data indicate that lactogenic activity in late pregnancy of DIO dams is not different to controls and is unlikely to play a major role in impaired onset of normal postpartum maternal behaviour.

Genomic Selection for Dairy Cattle Behaviour Considering Novel Traits in a Changing Technical Production Environment.

Cow behaviour is a major factor influencing dairy herd profitability and is an indicator of animal welfare and disease. Behaviour is a complex network of behavioural patterns in response to environmental and social stimuli and human handling. Advances in agricultural technology have led to changes in dairy cow husbandry systems worldwide. Increasing herd sizes, less time availability to take care of the animals and modern technology such as automatic milking systems (AMSs) imply limited human-cow interactions. On the other hand, cow behaviour responses to the technical environment (cow-AMS interactions) simultaneously improve production efficiency and welfare and contribute to simplified "cow handling" and reduced labour time. Automatic milking systems generate objective behaviour traits linked to workability, milkability and health, which can be implemented into genomic selection tools. However, there is insufficient understanding of the genetic mechanisms influencing cow learning and social behaviour, in turn affecting herd management, productivity and welfare. Moreover, physiological and molecular biomarkers such as heart rate, neurotransmitters and hormones might be useful indicators and predictors of cow behaviour. This review gives an overview of published behaviour studies in dairy cows in the context of genetics and genomics and discusses possibilities for breeding approaches to achieve desired behaviour in a technical production environment.

Protecting the future child: Foetal alcohol spectrum disorder, easy rescue and the regulation of maternal behaviour.

This paper argues that social contexts of inequality are crucial to understanding the ethics of gestational harm and responsibility. Recent debates on gestational harm have largely ignored the social context of gestators, including contexts of inequality and injustice. This can reinforce existing social injustices arising from colonialism, socio-economic inequality and racism, for example, through increased regulation of maternal behaviour. To demonstrate this, I focus on the related notions of the 'future child' and an obligation of easy rescue, which have been used to discuss the ethics of gestational harm in the context of alcohol consumption during gestation and foetal alcohol spectrum disorder (FASD). I use a feminist perspective to evaluate these ideas and conclude that anyone concerned with remediation of social injustice has good reason to be suspicious of the notion of the future child in the context of gestational harm.

Sows' Responses to Piglets in Distress: An Experimental Investigation in a Natural Setting.

Domestic pigs (Sus scrofa) possess complex socio-cognitive skills, and sows show high inter-individual variability in maternal behaviour. To evaluate how females-reared under natural conditions-react to the isolation calls of their own piglets or those of other females, we conducted observations and experimental trials. In January-February 2021, we conducted all-occurrences sampling on affiliation, aggression, and lactation (daily, 7:30-16:30 h) on six lactating and four non-lactating females at the ethical farm Parva Domus (Turin, Italy). The trials (30 s each, n = 37/sow) consisted of briefly catching and restraining a piglet. We recorded the sow response (none/reactive/proactive movement towards the piglet; self-directed anxiety behaviours such as body shaking) before and during the trial and under control conditions. Increased levels of anxiety behaviour in sows were accompanied by an increased frequency of responses. Less aggressive sows and lactating sows showed the highest frequencies of response. Finally, the isolation calls' maximum intensity had an influence on the type of response observed, with higher proactive response frequencies following lower intensity isolation calls. Our results suggest that being under lactation could play a key role in increasing sow response levels and that specific acoustic features may influence the response.

Prevalence of lactational oestrus in cats and consequences for kittens.

OBJECTIVES: Although lactation and suckling suppresses fertility in most mammals, some feline breeders have reported spontaneous oestrus during lactation, causing distress to kittens. This led the Official French Feline Pedigree Registry (Livre Officiel des Origines Félines - LOOF) to send a questionnaire to cat breeders requesting data on their last three litters. The aim of this study was to investigate the prevalence of lactational oestrus, its impact on litters and potential associations with litter size, age, parity, breed and seasonality. METHODS: Answers from 108 breeders were collected, providing data on 238 litters in 23 different breeds. Data were also collected on successive litters from multiparous queens (n = 20) and were analysed separately from the 195 independent births. RESULTS: Of the 195 independent births with complete data sets, 96 (49%) queens came into oestrus during lactation, 37 (38%) of which were associated with loss of maternal interest (n = 20), milk quality variation (n = 2), clotted milk (n = 3), reduced milk quantity (n = 13), which in kittens led to reduced weight (n = 6), diarrhoea (n = 9), vomiting (n = 4), nausea (n = 2) or death (n = 4), and bottle feeding (n = 2), early weaning (n = 4) or modified litter behaviour (n = 1). A significant association was found between small litter size (one or two kittens) and the onset of lactational oestrus (P = 0.007) and between births occurring in February, March and April and lactational oestrus (P = 0.005); there was no association with age or breed. CONCLUSIONS AND RELEVANCE: Breeders perceived a relationship in 38% of cases of lactational oestrus with maternal disinterest, clotted milk, reduced milk yield and in kittens, weight loss, vomiting, diarrhoea or even death. An association between small litter size and lactational oestrus was found, as well as with births occurring between February and April. Breeders presenting with at-risk females should be warned. Conservative and preventive measures such as contraceptive options are discussed as a possible therapy.

Postnatal melatonin administration to stressed dams for ameliorating risk-taking behaviour in rat pups through maternal care improvement.

BACKGROUND AND AIM: Anxiety often occurs both concurrently and sequentially in childhood and adolescence in association with prenatal stress, which may reduce the quality of maternal care and then cause mood disorders among children in later life. Against this background, melatonin, as a powerful antioxidant, was used in the present study to ameliorate risk-taking behaviour induced by pure maternal care in rat pups. MATERIALS AND METHODS: The Wistar rat dams recruited in this study were exposed to restraint stress from gestational day (GD) 11 until delivery. They further received melatonin (10 mg/kg) during the postnatal days (PNDs) 0-7 by intraperitoneal (IP) injections at 4:00 PM. The pregnant rats were then divided into four groups, namely, control, stress, stress + melatonin and melatonin, and their maternal behaviour and corticosterone levels were measured. In the offspring, the outcomes of some behavioural tasks, including the elevated plus-maze (EPM) and open-field (OF) tests were ultimately assessed. RESULTS: The study results revealed that the quantity and quality of maternal care significantly declined and the plasma corticosterone levels compounded in the stressed dams. Melatonin treatment, however, improved their nursing behaviour and reduced their plasma corticosterone levels. The offspring performance in two tasks also showed an upward trend in risk-taking behaviour in the stress group, and melatonin administration ameliorated the effects of stress and lessened their anxiety-like behaviour. CONCLUSION: It was concluded that prenatal restraint stress could impair stress responses and quality of maternal care, whereas postnatal melatonin administration potentially contributed to the normalization of stress reaction and anxiolysis.

Pups and prolactin are rewarding to virgin female and pregnant mice.

Maternal interactions with offspring are highly rewarding, which reinforces expression of essential caregiving behaviours that promote offspring survival. In rats, the rewarding effect of pups depends on reproductive state, with lactating females specifically developing strong preferences for pup-associated contexts. Whether this also occurs in mice is unknown, hence we aimed to characterise pup-related preference across reproductive states in female mice. In a conditioned place preference (CPP) test, pups were a rewarding stimulus to female mice prior to lactation, with virgin and pregnant females developing a preference for a pup-associated context. We have previously shown that lactogenic hormones, acting through the prolactin receptor (Prlr), play an important role in maternal motivation. Here, we aimed to investigate whether Prlr action is important for pup-related reward behaviour in mice. We showed that prolactin itself had a reinforcing effect in a CPP test, and that exposure to pups increased blood prolactin levels in virgin female mice. Prlr expression in CamKIIα-expressing neurons and GABAergic neurons has previously been shown to be important for different aspects of parental behaviour. However, we found that conditional Prlr deletion from either of these neuronal populations did not disrupt the development of a preference for pup-associated contexts in pregnant female mice, indicating that lactogenic action on these populations is not necessary for the rewarding effect of pups. Together, these data show that while lactogenic hormones likely contribute to a rewarding effect of pups, their action on two key neuronal populations is not necessary for this effect in female mice.

Piglets' behaviour and performance in relation to sow characteristics.

The importance of maternal care in commercial pig production is largely ignored. The sow has little possibility to interact with her piglets, and piglets are often subjected to early weaning or artificial rearing. This study aimed to investigate aspects of physiological and behavioural maternal provisioning that contribute to offspring outcomes. We hypothesised that better maternal care and nutritional provisioning would relate positively to piglet immunity, growth and behaviour. Nineteen sows and their litters were studied in free-farrowing pens. Oxytocin and tumour necrosis factor-α in colostrum/milk and salivary cortisol were sampled from sows throughout lactation. Sows were assessed for dominance rank, response to handling, maternal defensiveness, suckling initiation and termination, posture and sow-piglet contact. Piglets were weighed, measured for body mass index (BMI) and sampled for blood (Immunoglobulin G; at birth). After weaning, they experienced a human approach test (HAT) and novel object test. Correlations were explored between individual sow characteristics, individual piglet outcomes, and between sow characteristics and piglet outcomes averaged by litter. Significant correlations between sow and piglet factors were analysed at the litter level in mixed models with piglet outcomes as response variables and sow characteristics as predictor variables, while accounting for sow parity, litter size and batch. Litters grew faster when their sow had lower cortisol values (P = 0.03), while sows with lower cortisol levels had more successful suckling bouts and engaged in greater amounts of sow-piglet contact. Litters had a lower BMI at weaning when the sow had a higher milk fat percentage at d3. Litters of the most dominant sows took longer to approach the human in the HAT, while litters of sows with higher cortisol at d0 took longer to approach the novel object when assessed on correlations (r = 0.82, P < 0.001) but not when the model accounted for parity and litter size (P = 0.35). Only some of the measured nutritive and non-nutritive sow factors influenced litter performance and behaviour, with parity and litter size also playing a role. Given the continued increase in litter size, but also the interest in loose-housed lactation pens for sows, further research on sows' maternal investment and how it can be optimised is warranted.

Perceptions of Mothers about Support and Self-Efficacy in Breastfeeding: A Qualitative Study.

Breastfeeding is a complex process influenced by different personal and social factors which will determine both the initiation and the resilience for its maintenance. The aim is to identify the beliefs and expectations of mothers concerning breastfeeding to determine the perception of their self-efficacy and the influence on the management of their babies' feeding. A qualitative study through semi-structured interviews was carried out. The sample size was defined by the saturation criteria. Twenty-two women participated, eleven were from an urban environment and eleven were from a rural environment. Mothers' knowledge of breastfeeding, their expectations of that process, their experience, and their strategies for overcoming problems associated with initiating, establishing, and continuing breastfeeding were influenced by the role of nurses and midwives in supporting their perception of self-efficacy. Likewise, maternity policies are important for the continuance of exclusive breastfeeding. This study shows the complexity of the initiation and establishment of breastfeeding and the existence of several social factors surrounding these moments. Furthermore, it demonstrates the importance and reference of nurses and midwives and the role of State maternity policies.

Enhanced pup retrieval behaviour in a mouse model of polycystic ovary syndrome.

Polycystic ovary syndrome (PCOS) is the most common endocrinopathy to affect women of reproductive-age world-wide. Hyperandrogenism is both a hallmark feature of PCOS, and is hypothesised to be an underlying mechanism driving the development of the condition in utero. With circulating hormones known to profoundly influence maternal responses in females, we aimed to determine whether maternal behaviour is altered in a well-described prenatally androgenised (PNA) mouse model of PCOS. Mouse dams were administered with dihydrotestosterone or vehicle on days 16, 17 and 18 of pregnancy. Maternal responses were assessed in both the dihydrotestosterone-injected dams following parturition and in their adult female PNA offspring. Exposure of dams to excess androgens during late pregnancy had no detrimental effects on pregnancy outcomes, including gestation length, pup survival and gestational weight gain, or on subsequent maternal behaviour following parturition. By contrast, PNA virgin females, modelling PCOS, exhibited enhanced maternal behaviour when tested in an anxiogenic novel cage environment, with females rapidly retrieving pups and nesting with them. In comparison, most control virgin females failed to complete this retrieval task in the anxiogenic environment. Assessment of progesterone receptor and oestrogen receptor α immunoreactivity in the brains of virgin PNA and control females revealed increased numbers of oestrogen receptor α positive cells in the brains of PNA females in regions well known to be important for maternal behaviour. This suggests that increased oestrogenic signalling in the neural circuit that underlies maternal behaviour may be a possible mechanism by which maternal behaviour is enhanced in PNA female mice.

Effect of maternal parenting style on child behaviour and its management strategies in dental office: A pilot study.

AIM: To evaluate the effect of maternal parenting style on child behaviour and consequent management strategies in dental office. MATERIALS AND METHODS: Eighty child-mother pairs were included in the study. Before the start of the treatment, Maternal parenting style was evaluated utilising Primary Caregivers Practices Report, while child behaviour along with his/her perception towards dental treatment were observed using Frankl Behaviour Rating Scale and Sound Eye Motor scale, respectively. Also the behaviour management strategy utilised for the child was noted and its correlation to both parenting style and child behaviour was evaluated. RESULTS: A statistically significant correlation (p-value = 0.00) was observed between the maternal parenting style and child behaviour displayed during dental treatment. Also, a positive correlation (p-value = 0.00) was observed between behaviour displayed by the children and behaviour management strategy used in the dental operatory. CONCLUSION: The clinicians can predict the type of behaviour the child might display and the behaviour management strategy to be utilised for a specific child based on the maternal parenting style.

Does Nursing Behaviour of Sows in Loose-Housing Pens Differ from That of Sows in Farrowing Pens with Crates?

Sows confined to farrowing crates are restricted in performing natural behaviour such as maternal behaviour. Loose-housing farrowing pens (LH) and farrowing pens with crates (FC) were compared regarding sows' nursing behaviour via video analyses over four weeks per batch (one day per week). Nursing frequency was similar in LH and FC pens (1.25 ± 0.82 vs. 1.19 ± 0.75 nursings/sow/hour; p > 0.05). However, nursing duration differed between the two systems (LH: 5.7 ± 4.6 min vs. FC: 7.0 ± 5.0 min; odds ratio (OR) 1.168, p = 0.011). In LH pens, more nursing bouts were sow-terminated than in FC pens (OR 0.427, p = 0.001). The probability of sow-terminated nursing occurring increased from week 1 to week 4 (OR 3.479, adjusted p (padj) < 0.001), while that of observing unnursed piglets decreased from week 1 to week 4 (OR 0.301, padj < 0.001) and rose with increasing litter size (OR 1.174, p = 0.010). We conclude that nursing behaviour was affected by the farrowing system, with shorter nursing duration and more nursing terminations by the sow in LH than in FC pens. Since this corresponds to the nursing behaviour of sows in semi-natural conditions, it can be assumed that sows in LH pens are more likely to exhibit natural nursing behaviour.

Maternal sensitivity and intrusiveness in early childhood as predictors of children's weight at school age.

BACKGROUND: While previous research indicates that low maternal sensitivity in mother-child interactions puts children at risk of overweight and obesity, maternal intrusiveness has rarely been investigated in association with children's weight. We investigated whether maternal sensitivity and intrusiveness in early childhood predict children's increased body mass index standard deviation scores (BMI-SDS) at school age. BMI-SDS are standardized for age and gender with respect to a reference standard. METHODS: At baseline (t1), we assessed maternal sensitivity and (non-)intrusiveness of 116 mothers with their children (48.3% female) aged 5-47 months (M = 24.00, SD = 11.36) using the emotional availability scales. We obtained anthropometric data for mothers at t1 by measuring height and weight in the laboratory and for children at birth assessed by medical staff. Six years later (t2) we obtained anthropometric data for children in the laboratory or based on parental report. Linear regression analyses were run with child BMI-SDS at t2 as outcome and sensitivity and (non-)intrusiveness as predictors, adjusting for confounders and exploring child age and gender as moderators. RESULTS: Maternal sensitivity only negatively predicted children's BMI-SDS in girls, while maternal intrusiveness predicted higher child BMI-SDS at school age regardless of child gender. The effect of maternal non-intrusiveness remained significant when controlling for confounders. CONCLUSION: Maternal intrusiveness in early childhood seems to represent a risk factor for increased BMI-SDS in children, while lower maternal sensitivity tends to be a risk factor for increased BMI-SDS in girls. This may have implications for prevention or intervention programmes.

Maternal reactivity of ewes at lambing is genetically linked to their behavioural reactivity in an arena test.

In sheep, the bond between the dam and her lambs is established during the first hours of a lamb's life. Genetic variability for behavioural reactivity of ewes assessed in an arena test performed 24 hr after lambing has already been reported. However, there is no evidence that this reactivity represents the ewe's maternal reactivity at lambing in outdoor conditions. The objective of this study was to investigate whether or not the behavioural reactivity of ewes in the arena test is genetically related to their maternal reactivity measured at lambing. A total of 935 Romane ewes were studied. The maternal reactivity of ewes at the outdoor lambing site was recorded in response to a human approach and to the handling of the lambs. Their behavioural reactivity was also recorded 24 hr post-lambing in the arena test that involved a separation from the litter and a human presence. Flight distance, aggressive reaction, time to restore contact with the litter, maternal behaviour scores and vocalizations recorded at the lambing site were heritable (0.12-0.34). All of these behaviours were genetically correlated with the behavioural reactivity in the arena test. The highest genetic correlations (from 0.60 to 0.90) were found amongst maternal behavioural scores, flight distance and high-pitched bleats. In conclusion, behavioural reactivity in the arena test can be used to assess early maternal reactivity in standardized conditions. Phenotyping of ewes' behavioural reactivity with a simplified arena test can be performed for genetic improvement in maternal behaviour in sheep.

A survey on management and housing of peri-parturient dairy cows and their calves.

Housing and management around the time of calving impact dairy cow behaviour, health and welfare, but little is known about current practice. The aim was to provide an overview of current calving practice and the study describes the main calving housing and management based on replies to an online questionnaire by 42 dairy cattle experts in 28 countries, or regions, in Europe, Canada and USA. The survey suggests that in the majority of countries and regions included in this study, dairy cows typically calve in indoor calving facilities; either individual pens, group pens or a system where the cow is moved from a group pen into an adjacent individual pen before calving. Regarding individual calving pens, the survey suggests that in the majority of countries and regions included in this study, most pens have open sides and offer cows no opportunity to isolate, although research shows that a secluded corner of an individual pen creates a preferred calving site. Further, the survey suggests that when cows calve in individual calving pens or tie-stalls, they are often moved there with signs of imminent calving, although research shows that this practice increases the duration of calving and it is recommended to move cows before their expected calving time. Regarding group pens, none of the 42 respondents replied that group pens typically offer cows the opportunity to isolate at calving. Recent research suggests that when cows calve in a secluded area of a group calving pen, this reduces the risk of failure of passive transfer of immunity. Regarding calving facilities where group pens are combined with adjacent individual pens, this was reported to be the most typical in 10 of the 24 countries and regions with indoor calving sections covered by the survey. The same concerns regarding when the cow is moved from the group pen to an individual pen apply, as outlined above. Irrespective of pen type, the most frequently reported surface was deep bedded straw and the most frequent type of separation between pens was open sides. Cow-calf separation within 12 hours of birth, and thereafter individual housing of calves combined with milk feeding via a teat bucket or bar was indicated the most frequent management. The survey presents experts' evaluations of current practice of housing and management of peri-parturient dairy cows and their calves, and suggests that there is a discrepancy between current calving management and housing and recommendations based on recent research.

Why do some primate mothers carry their infant's corpse? A cross-species comparative study.

Non-human primates respond to the death of a conspecific in diverse ways, some of which may present phylogenetic continuity with human thanatological responses. Of these responses, infant corpse carrying by mothers (ICC) is the most frequently reported. Despite its prevalence, quantitative analyses of this behaviour are scarce and inconclusive. We compiled a database of 409 published cases across 50 different primate species of mothers' responses to their infants' deaths and used Bayesian phylogenetic regressions with an information-theoretic approach to test hypotheses proposed to explain between- and within-species variation in ICC. We found that ICC was more likely when the infant's death was non-traumatic (e.g. illness) versus traumatic (e.g. infanticide), and when the mother was younger. These results support the death detection hypothesis, which proposes that ICC occurs when there are fewer contextual or sensory cues indicating death. Such an interpretation suggests that primates are able to attain an awareness of death. In addition, when carried, infant age affected ICC duration, with longer ICC observed for younger infants. This result suggests that ICC is a by-product of strong selection on maternal behaviour. The findings are discussed in the context of the evolution of emotion, and implications for evolutionary thanatology are proposed.