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Pathog Glob Health ; 112(8): 415-420, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30433859

RESUMO

Virulence and antibiotic resistance properties related to different Escherichia coli phylogenetic groups have not been studied in detail in Mexico. We aimed to identify patterns of virulence genes and multidrug resistance in phylogenetic groups of uropathogenic strains (UPEC). Strains of E. coli were isolated from outpatients with urinary tract infections (UTIs), who went to unit of the public health sector in the State of Mexico. E. coli virulence markers and phylogenetic groups were identified by PCR. Susceptibility to 12 antimicrobials was determined by Kirby-Bauer. E. coli was identified in 60.4% (n = 194) of the patients with UTIs. Phylogroups B2 51% (n = 99), A 13.4% (n = 26) and B1 10.3% (n = 20) were the most frequent. Resistance to three or up to eleven antibiotics was detected in most phylogroups (n = 188). The genes fimH (n = 146), feoB (n = 179), iutA (n = 178), sitA (n = 121), fyuA (n = 99), and traT (n = 142) were mainly detected in strains of phylogroups B2, A, B1, C, and D. Seventy-two patterns of virulence markers were distributed across eight E. coli phylogenetic groups. A high frequency of virulence markers and the multiple antibiotic resistance phenotypes was observed in the phylogroups. The genes of extended-spectrum ß-lactamases (ESBLs) found with higher frequency among UPEC strains were blaTEM, blaSHV y blaCTX-M group 1, CIT (plasmid-mediated AmpC ß-lactamase), and blaOXA-like. In conclusion, our findings show the importance of surveillance, permanent monitoring, and particularly controlled prescription of antibiotics by physicians in the social security health system to reduce the spread of highly virulent UPEC strains that are resistant to multiple antimicrobial agents.


Assuntos
Farmacorresistência Bacteriana Múltipla , Infecções por Escherichia coli/microbiologia , Infecções Urinárias/microbiologia , Escherichia coli Uropatogênica/efeitos dos fármacos , Escherichia coli Uropatogênica/patogenicidade , Fatores de Virulência/análise , Adulto , Idoso , Antibacterianos/farmacologia , Infecções por Escherichia coli/epidemiologia , Feminino , Variação Genética , Genótipo , Humanos , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Pacientes Ambulatoriais , Filogenia , Reação em Cadeia da Polimerase , Infecções Urinárias/epidemiologia , Escherichia coli Uropatogênica/classificação , Escherichia coli Uropatogênica/isolamento & purificação , Virulência , Fatores de Virulência/genética , Adulto Jovem
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