Obtaining and characterizing polyphenol extracts based on anthocyanins from Melinis minutiflora inflorescences and Plinia cauliflora fruits and application in gelatins.

Anthocyanins are a class of compounds potentially used as food dyes. Thus, this study aimed to obtain and characterize natural extracts from Melinis minutiflora inflorescence (M), Plinia. cauliflora peel (JP) and P. cauliflora peel and seeds (JPS) and apply them as natural food dyes in gelatins. The extracts did not show statistically significant differences in pH values and water activity. The M and JPS extracts showed similar values of anthocyanins and total phenolic compounds and were higher than those from the JP extract. The M and JPS extracts showed a bathochromic effect, which was not observed for the JP extract. The bathochromic effect may indicate a possible complexation of anthocyanins. The color composition analysis revealed that the JP extract has a higher absorbance at a wavelength of 520 nm, indirectly suggesting the presence of more monomeric anthocyanins in its composition. The extract application test in gelatin did not change the texture properties of the gelatins. In addition, our findings revealed that the JPS extract had the best color stability after ten days of analysis, indicating that anthocyanin complexation with the phenolic compounds of P. cauliflora seeds contributed more effectively to anthocyanin stability in the model used.

Effects of liquid extract from Plinia cauliflora fruits residues on Chinese hamsters biochemical parameters / Efeitos do extrato líquido de resíduos de frutos de Plinia cauliflora sobre os parâmetros bioquímicos de hamsters chineses

Plinia cauliflora (Mart.) Kausel, popularly known as jabuticaba, is rich in polyphenols. Phenolic compounds exhibit several biological properties, which reflect on biomarkers such as biochemical parameters. In the present study, we evaluated the plasmatic levels of glucose, total cholesterol, HDL-cholesterol, triglycerides, and uric acid of Chinese hamsters fed for 45 days with a regular diet or cholesterol-enriched diet supplemented with a liquid extract obtained from P. cauliflora fruits residues standardized in ellagic acid and total phenolic compounds. The results showed that the concentrated extract obtained from jabuticaba residues increased the glycemia of animals fed with a regular diet and reduced the plasmatic uric acid levels of animals fed with a cholesterol-enriched diet. Since hyperuricemia is considered to be a significant risk factor of metabolic disorders and the principal pathological basis of gout, the liquid extract from P. cauliflora fruits residues would be a promising candidate as a novel hypouricaemic agent for further investigation.

Plinia cauliflora (Mart.) Kausel, popularmente conhecida como jabuticaba, é rica em polifenois. Os compostos fenólicos apresentam diversas propriedades biológicas, que refletem em biomarcadores, como os parâmetros bioquímicos. No presente estudo, avaliamos os níveis plasmáticos de glicose, colesterol total, HDL-colesterol, triglicerídeos e ácido úrico em hamsters chineses alimentados por 45 dias com dieta regular ou dieta enriquecida com colesterol suplementada com extrato líquido obtido de resíduos de frutos de P. cauliflora padronizado em ácido elágico e compostos fenólicos totais. Os resultados mostraram que o extrato concentrado obtido dos resíduos de jabuticaba aumentou a glicemia dos animais alimentados com dieta regular e reduziu os níveis plasmáticos de ácido úrico dos animais alimentados com dieta rica em colesterol. Uma vez que a hiperuricemia é considerada um fator de risco significativo de distúrbios metabólicos e a principal base patológica da gota, o extrato líquido dos resíduos de frutas de P. cauliflora seria um candidato promissor como um novo agente hipouricêmico para investigação posterior.

Effects of liquid extract from Plinia cauliflora fruits residues on Chinese hamsters biochemical parameters

Abstract Plinia cauliflora (Mart.) Kausel, popularly known as jabuticaba, is rich in polyphenols. Phenolic compounds exhibit several biological properties, which reflect on biomarkers such as biochemical parameters. In the present study, we evaluated the plasmatic levels of glucose, total cholesterol, HDL-cholesterol, triglycerides, and uric acid of Chinese hamsters fed for 45 days with a regular diet or cholesterol-enriched diet supplemented with a liquid extract obtained from P. cauliflora fruits residues standardized in ellagic acid and total phenolic compounds. The results showed that the concentrated extract obtained from jabuticaba residues increased the glycemia of animals fed with a regular diet and reduced the plasmatic uric acid levels of animals fed with a cholesterol-enriched diet. Since hyperuricemia is considered to be a significant risk factor of metabolic disorders and the principal pathological basis of gout, the liquid extract from P. cauliflora fruits residues would be a promising candidate as a novel hypouricaemic agent for further investigation.

Resumo Plinia cauliflora (Mart.) Kausel, popularmente conhecida como jabuticaba, é rica em polifenois. Os compostos fenólicos apresentam diversas propriedades biológicas, que refletem em biomarcadores, como os parâmetros bioquímicos. No presente estudo, avaliamos os níveis plasmáticos de glicose, colesterol total, HDL-colesterol, triglicerídeos e ácido úrico em hamsters chineses alimentados por 45 dias com dieta regular ou dieta enriquecida com colesterol suplementada com extrato líquido obtido de resíduos de frutos de P. cauliflora padronizado em ácido elágico e compostos fenólicos totais. Os resultados mostraram que o extrato concentrado obtido dos resíduos de jabuticaba aumentou a glicemia dos animais alimentados com dieta regular e reduziu os níveis plasmáticos de ácido úrico dos animais alimentados com dieta rica em colesterol. Uma vez que a hiperuricemia é considerada um fator de risco significativo de distúrbios metabólicos e a principal base patológica da gota, o extrato líquido dos resíduos de frutas de P. cauliflora seria um candidato promissor como um novo agente hipouricêmico para investigação posterior.

Fruit and Agro-Industrial Waste Extracts as Potential Antimicrobials in Meat Products: A Brief Review.

The use of antimicrobials in meat products is essential for maintaining microbiological stability. The reformulation by substituting synthetic additives for natural ones is an alternative to provide cleaner label products. Therefore, this work performed a literature search about extracts from fruits and agro-industrial waste with antimicrobial activity that can be applied in meat products. Jabuticaba waste extracts are excellent sources of anthocyanins with antimicrobial and pigmentation potential, capable of being applied in meat products such as fresh sausage, without compromising sensory attributes. Residue from grapes is rich in antimicrobial phytochemicals, mainly catechins, epicatechins, gallic acid and procyanidins. Extracts from different grape by-products and cultivars showed inhibition of Staphylococcus aureus, Listeria monocytogenes, Pseudomonas aeruginosa, Escherichia coli O157: H7 and other bacterial strains. Antimicrobial effects against L. monocytogenes, Bacillus cereus, S. aureus and E. coli O157: H7 were identified in Opuntia extracts. In addition, its application in hamburgers reduced (p < 0.05) aerobic mesophilic bacteria, Enterobacteriaceae and Pseudomonas sp. counts, and at a concentration of 2.5%, improved the microbiological stability of salami without causing sensory and texture changes. These data reinforce the possibility of substituting synthetic preservatives for natural versions, a growing trend that requires researching effective concentrations to maintain the sensory and technological properties.

Four New Phloroglucinol-Terpene Adducts from the Leaves of Myrciaria cauliflora.

Myrcauones A-D (1-4), four new phloroglucinol-terpene adducts were isolated from the leaves of Myrciaria cauliflora. Their structures with absolute configurations were elucidated by combination of spectroscopic analysis, single crystal X-ray diffraction, and electronic circular dichroism (ECD) calculations. Compound 1 was a rearranged isobutylphloroglucinol-pinene adduct featuring an unusual 2,3,4,4a,10,11-hexahydro-1H-3,11a-methanodibenzo[b,f]oxepin backbone. Compound 4 showed moderate antibacterial activity against Gram-positive bacteria including multiresistant strains.

Jaboticabin and Related Polyphenols from Jaboticaba ( Myrciaria cauliflora) with Anti-inflammatory Activity for Chronic Obstructive Pulmonary Disease.

Myrciaria cauliflora (jaboticaba) is an edible fruit common in Brazil that has been used for treating respiratory diseases, including chronic tonsillitis and asthma. This study explores the distribution of an anti-inflammatory depside, jaboticabin, in different parts of the jaboticaba plant as well as major polyphenols from the wood of jaboticaba, some with biological activity similar to jaboticabin. The peel of the fruit was found to be the major source of jaboticabin. This is the first phytochemical study of the wood of M. cauliflora. The antioxidant-activity-guided fractionation strategy successfully identified 3,3'-dimethylellagic acid-4- O-sulfate from jaboticaba wood. This ellagic acid derivative, in a manner similar to jaboticabin, showed antiradical activity and inhibited the production of the chemokine interleukin-8 after treating the human small airway epithelial cells with cigarette smoke extract. The human intestinal Caco-2 cell studies demonstrated the jaboticabin transport in vitro. The polyphenols, jaboticabin and 3,3'-dimethyellagic acid-4- O-sulfate, from jaboticaba were both found to exhibit anti-inflammatory activities, thus suggesting the potential use of these compounds or even the fruits themselves for chronic obstructive pulmonary disease.

Efficacy of photoactivated Myrciaria cauliflora extract against Staphylococcus aureus infection - A pilot study.

Staphylococcus aureus is one of the major microorganisms that cause human diseases, leading from mild skin infections to serious diseases. With the use of semi-synthetic penicillins, methicillin-resistant strains called Methicillin-resistant Staphylococcus aureus (MRSA) have emerged, whose resistance pattern extends to other beta-lactam antibiotics. It has already been shown that photodynamic therapy is capable of inactivating MRSA as the laser excites the photosensitizer responsible for transferring its electrons to the molecular oxygen, generating extremely reactive molecules, such as singlet oxygen, being these reactive components the chemicals that promote the bacterial clearance. Thus, the research aiming at the development of new photosensitizers becomes important, especially to increase the amount of therapeutic resources available for the treatment of persistent infections related to this bacterium. In this context, Myrciaria cauliflora is a plant that has antimicrobial action and there are no reports of the use of its crude extract as a photosensitizer in antimicrobial photodynamic therapy. In that way, this work conveys an innovative way of the use of M. cauliflora extract as a photosensitizer, comprising its use as an antimicrobial agent when activated by light, against S. aureus. In vitro tests were performed where it was observed that after the photoactivation with blue LED light, the extract presented an augment in its antimicrobial activity, together with production of singlet oxygen. In the model of intradermal infection in Balb/c mice, a reduction in bacterial load was also detected, with raised expression of TNF-α, IL-17A, and MPO. Here, we demonstrate that the extract of M. cauliflora has photosensitizing action, promoting the production of singlet oxygen, besides IL-17A, TNF-α, and MPO.

Identification of candidate genes involved in anthocyanin accumulation in the peel of jaboticaba (Myrciaria cauliflora) fruits by transcriptomic analysis.

Jaboticaba is a grape-like fruit that accumulates high levels of anthocyanins in the peel and is proposed as a good source of functional pigments. However, the molecular mechanisms underlying anthocyanin accumulation in jaboticaba peel remains to be elucidated. In this study, we employed RNA-seq technique to compare the transcriptomic differences between green-colored and black-colored jaboticaba peels. Over 5 million high-quality reads were assembled into 62,190 unigenes with an average length of 737 bp, 29,320 (47.15%) of them were annotated by public databases. 2152 unigenes were found to be differentially expressed (830 upregulated and 1322 downregulated). Gene ontology analysis and pathway enrichment annotation revealed that 18 differentially expressed genes encode phenylalanine ammonialyase, 4-coumaroyl:CoA-ligase, chalcone synthase, flavanone 3-hydroxylase, flavonoid 3'-hydroxylase, anthocyanidin synthase, UDP-glucose: flavonoid 3-O-glucosyltransferase, glutathione S-transferase, Cytochrome b5 were associated with anthocyanin biosynthesis. Additionally, 54 differentially expressed transcription factors were identified. Furthermore, the expression of genes involved in biosynthesis and signal transduction of ethylene and abscisic acid were negatively and positively correlated with that of anthocyanin pathway genes and anthocyanin accumulation, respectively. Quantitative reverse transcription PCR analysis of candidate genes showed trends similar to those in the RNA-seq analysis. McMYB, a homolog of AtMYB113, induced anthocyanin accumulation in tobacco leaves when co-infiltrated PsbHLH3. These results will contribute to further understanding of the molecular mechanisms regulating anthocyanin accumulation in jaboticaba peel.

Small Brazilian wild fruits: Nutrients, bioactive compounds, health-promotion properties and commercial interest.

Brazilian berries present great nutritional, functional and economic characteristics comparable to temperate berries. They constitute an important innovation domain for the food, pharmaceutical and cosmetic industries, due to their positive health effects and market potential. The main objective of this review was to describe the physicochemical, nutritional and biological aspects of six Brazilian small native wild fruits from the Arecaceae (açaí, buriti and pupunha), Mirtaceae (camu-camu and jaboticaba) and Malpighiaceae (murici) families, highlighting their antioxidant, anti-lipidaemic, anti-inflammatory, antiproliferative, and antigenotoxic potential among others. It also discussed some relevant topics for new studies that could be of benefit to industry and improve the local economy where these fruits are found. Thus, the dissemination of the works already carried out with these fruits strategically relevant can stimulate new lines of research to consolidate this new field for the food industries.

Polyphenol and Ellagitannin Constituents of Jabuticaba (Myrciaria cauliflora) and Chemical Variability at Different Stages of Fruit Development.

A new ellagitannin named cauliflorin (1), seven known hydrolyzable tannins (2-8), and six known phenolics (9-14) were isolated from jabuticaba. Compounds 2-8 had not been previously isolated from M. cauliflora fruits. The jabuticaba fruit was analyzed at four developmental stages for ellagitannins 1, 3, 7, and 8, phenolic acids 11 and 12, anthocyanins, organic acids, and sugars via HPLC-UV-DAD and NMRq. The content of ellagitannins and organic acids declined during fruit development, whereas at full ripeness sugar and anthocyanin levels underwent a sharp increase and were mainly constituted by fructose and cyanidin-3-O-glucose, respectively. Ellagitannins' profile varied considerably among fruit tissues, with pedunculagin (3), castalagin (7), and vescalagin (8) mostly concentrated in jabuticaba seeds, whereas cauliflorin (1) and anthocyanins accumulated in the peels. Changes in jabuticaba's phenolic compound contents were mostly influenced by fruit part (peel, pulp, and seed) rather than by degree of ripeness.

Jabuticaba-Induced Endothelium-Independent Vasodilating Effect on Isolated Arteries / Efeito Vasodilatador Independente do Endotélio Induzido pela Jabuticaba (M. cauliflora) em Artérias Isoladas

Abstract Background: Despite the important biological effects of jabuticaba, its actions on the cardiovascular system have not been clarified. Objectives: To determine the effects of jabuticaba hydroalcoholic extract (JHE) on vascular smooth muscle (VSM) of isolated arteries. Methods: Endothelium-denuded aortic rings of rats were mounted in isolated organ bath to record isometric tension. The relaxant effect of JHE and the influence of K+ channels and Ca2+ intra- and extracellular sources on JHE-stimulated response were assessed. Results: Arteries pre-contracted with phenylephrine showed concentration-dependent relaxation (0.380 to 1.92 mg/mL). Treatment with K+ channel blockers (tetraethyl-ammonium, glibenclamide, 4-aminopyridine) hindered relaxation due to JHE. In addition, phenylephrine-stimulated contraction was hindered by previous treatment with JHE. Inhibition of sarcoplasmic reticulum Ca2+ ATPase did not change relaxation due to JHE. In addition, JHE inhibited the contraction caused by Ca2+ influx stimulated by phenylephrine and KCl (75 mM). Conclusion: JHE induces endothelium-independent vasodilation. Activation of K+ channels and inhibition of Ca2+ influx through the membrane are involved in the JHE relaxant effect.

Resumo Fundamentos: Embora a jabuticaba apresente importantes efeitos biológicos, suas ações sobre o sistema cardiovascular ainda não foram esclarecidas. Objetivos: Determinar os efeitos do extrato de jabuticaba (EHJ) sobre o músculo liso vascular (MLV) em artérias isoladas. Métodos: Aortas (sem endotélio) de ratos foram montadas em banho de órgãos isolados para registro de tensão isométrica. Foram verificados o efeito relaxante, a influência dos canais de K+ e das fontes de Ca2+ intra- e extracelular sob a resposta estimulada pelo EHJ. Resultados: Artérias pré-contraídas com fenilefrina apresentaram relaxamento concentração-dependente (0,380 a 1,92 mg/mL). O tratamento com bloqueadores de canais de K+ (tetraetilamônio, glibenclamida, 4-aminopiridina) prejudicaram o relaxamento pelo EHJ. A contração estimulada com fenilefrina também foi prejudicada pelo tratamento prévio com EHJ. A inibição da Ca2+ATPase do reticulo sarcoplasmático não alterou o relaxamento pelo EHJ. Além disso, o EHJ inibiu a contração causada pelo influxo de Ca2+ estimulado por fenilefrina e KCl (75 mM). Conclusão: O EHJ induz vasodilatação independente do endotélio. Ativação dos canais de K+ e inibição do influxo de Ca2+ através da membrana estão envolvidas no efeito relaxante do EHJ.

Effect of plant extracts and a disinfectant on biological parameters and pathogenicity of the fungus Beauveria bassiana (Bals.) Vuill. (Ascomycota: Cordycipitaceae) / Ação de extratos vegetais e desinfetantes sobre parâmetros biológicos e patogenicidade do fungo Beauveria bassiana (Bals.) Vuill. (Ascomycota: Cordycipitaceae)

Abstract The fungus Beauveria bassiana is naturally found in poultry houses and causes high rates of mortality in Alphitobius diaperinus. Laboratory and field experiments have shown the potential of this fungus as an insect control agent. However, in poultry houses, bacteria as Salmonella, can be found and have been studied alternative control methods for this pathogen. Thus, this study aimed to evaluate the effect of plant extracts and a disinfectant on the fungus Beauveria bassiana (strain Unioeste 4). Conidial viability, colony-forming unit (CFU) counts, vegetative growth, conidia production, insecticidal activity of the fungus and compatibility were used as parameters in the evaluation of the effect of these products on the fungus. Alcoholic and aqueous extracts of jabuticaba (Myrciaria cauliflora (Mart.), guava (Psidium guajava (L.)), and jambolan (Syzygium cumini (L.), at concentrations of 10% as well as the commercial disinfectant, Peroxitane® 1512 AL, were evaluated at the recommended concentrations (RC), 1:200 (RC), 0.5 RC and 2 RC. There was a negative influence of alcoholic and aqueous extracts of jabuticaba, guava and three dilutions of Peroxitane on the viability of conidia. The CFUs and vegetative growth of the fungus were affected only by the Peroxitane (all dilutions). For conidial production, the aqueous extract of guava had a positive effect, increasing production, while the Peroxitane at the R and RC concentrations resulted in a negative influence. The mortality of A. diaperinus, caused by the fungus after exposure to these products, was 60% for the peracetic acid at 0.5 RC, and above 80% for the extracts. Thus, the results showed that all the extracts and Peroxitane at RC 0.5 are compatible with the fungus B. bassiana Unioeste 4, however only the extracts had a low impact on inoculum potential.

Resumo O fungo Beauveria bassiana é encontrado naturalmente em aviários de frango de corte, tendo sua eficácia como agente controlador do Alphitobius diaperinus, em condições de laboratório e campo. No entanto, nos aviários encontram-se também bactérias, como a Salmonella, para a qual vêm sendo pesquisadas alternativas de controle. Sendo assim, o objetivo do trabalho foi avaliar o efeito de extratos vegetais e um desinfetante com potencial de uso contra Salmonella spp., sobre os parâmetros biológicos do fungo B. bassiana isolado Unioeste 4. Foram avaliados extratos alcoólicos e aquosos de folhas de jabuticabeira (Myrciaria cauliflora (Mart.)), goiabeira (Psidium guajava (L.)), jamboleiro (Syzygium cumini (L.)), na concentração de 10% e também o desinfetante comercial Peroxitane®1512 AL na concentração recomendada – 1:200 (CR), 0,5 CR e 2CR. Foram avaliados a: germinação dos conídios, unidades formadoras de colônias (UFC), crescimento vegetativo, produção de conídios e efeito sobre a atividade inseticida do fungo contra adultos de A. diaperinus, bem como a compatibilidade entre produtos e o fungo. Verificou-se influência negativa dos extratos alcoólico e aquoso de jabuticabeira, goiabeira e das três diluições de Peroxitane sobre a viabilidade dos conídios. Já, a UFC e o crescimento vegetativo foram afetados apenas com Peroxitane (em todas as diluições). Para produção de conídios, o extrato aquoso de goiabeira teve efeito positivo, elevando a produção, enquanto as diluições recomendada e o dobro de Peroxitane mostraram influência negativa. Observou-se ainda que a mortalidade de A. diaperinus causada pelo fungo após a exposição aos produtos foi de 60% para o ácido peracético na 0,5 CR, já para os extratos foi acima de 80%. Assim, os resultados demonstraram que todos os extratos e o Peroxitane na 0,5 CR são compatíveis com o fungo B. bassiana Unioeste 4, porém apenas os extratos tiveram baixo impacto sobre o potencial de inóculo do fungo.

Myrciaria cauliflora extracts attenuate diabetic nephropathy involving the Ras signaling pathway in streptozotocin/nicotinamide mice on a high fat diet.

Diabetic nephropathy (DN) is a major cause of end-stage renal disease and its mortality is continuously increasing worldwide. Previous studies indicate that reactive oxygen species play an important role in high glucose-induced renal injury. Myrciaria cauliflora has been reported as a functional food rich in anthocyanins possessing anti-oxidative and anti-inflammatory properties. This study examined whether M. cauliflora extracts (MCE) can attenuate diabetic nephropathy progression in type 2 diabetes mellitus mice. First, the composition of the anthocyanins and polyphenols of MCE were determined by high-performance liquid chromatography and spectrophotometry. One hundred mg/kg of streptozotocin and 240 mg/kg nicotinamide were administered to C57BL/6J mice fed a high fat diet and varied concentrations of MCE. The plasma glucose concentration, body weight, oral glucose tolerance, blood pressure, renal ultrasound ultrasonic wave were monitored every 2 weeks. Following euthanasia, the kidneys of the mice were analyzed using hematoxylin-eosin, periodic acid Schiff, Masson's trichrome, and immunohistochemistry staining. The results showed that MCE stabilized the plasma glucose and indirectly improved insulin sensitivity in diabetic mice. In addition, diabetes-caused glomerular atrophy, accumulation of saccharide, and formation of collagen IV were recovered or reduced under treatment with MCE in diabetic mice. Our results indicate that MCE has beneficial effects in DN and the mechanism has been confirmed to inhibit Ras/PI3K/Akt and kidney fibrosis related proteins. This work illustrates the potential of MCE rich in anthocyanins and polyphenols as a natural food to inhibit DN.

Myrciaria cauliflora extract improves diabetic nephropathy via suppression of oxidative stress and inflammation in streptozotocin-nicotinamide mice.

Myrciaria cauliflora is a functional food rich in anthocyanins, possessing antioxidative and anti-inflammatory properties. Our previous results demonstrated M. cauliflora extract (MCE) had beneficial effects in diabetic nephropathy (DN) and via the inhibition of Ras/PI3K/Akt and kidney fibrosis-related proteins. The purpose of this study was to assess the benefit of MCE in diabetes associated with kidney inflammation and glycemic regulation in streptozotocin-nicotinamide (STZ/NA)-induced diabetic mice. Compared with the untreated diabetic group, MCE significantly improved blood glucose and serum biochemical characteristic levels. Exposure to MCE increased antioxidative enzyme activity and diminished reactive oxygen synthesis. Mice receiving MCE supplementation had reduced intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), monocyte chemoattractant protein 1 (MCP-1), colony stimulating factor 1 (CSF-1), interleukin-1ß (IL-1ß), IL-6 and tumor necrosis factor α (TNF-α) levels compared to the untreated diabetic mice. Inflammatory and fibrotic related proteins such as collagen IV, fibronectin, Janus kinase (JAK), phosphorylated signal transducer and activator of transcription 3 (STAT3), protein kinase C beta (PKC-ß), and nuclear factor kappa B (NF-κB) were also inhibited by MCE treatment in STZ/NA mice. These results suggest that MCE may be used as a hypoglycemic agent and antioxidant in Type 2 diabetic mice.

Atividade antioxidante em vinhos de jabuticaba e de uva / Antioxidant activity in wines made from jabuticaba and grape

Samples of red and white dry wines made from jaboticaba and grape were analyzed with the purpose of verifying the antioxidant activity of compounds present in the wines. Different volumes of duplicate samples were spectrophotometrically analysed in a ?-carotene/linoleic acid system, every 15 minutes, for two hours. It was observed that the red wine made from jabuticaba presented a better antioxidant activity and inhibition values close to the ones obtained with BHT (a synthetic antioxidant usedin the food industry), with inhibition around 65%. The kinetic study revealed that wines made from jabuticaba are good free-radical scavengers with F <1 in phase 1 of the oxidative chain reaction, with better results than the wines made from grapes. The jabuticaba wine is a good source of antioxidants. However, further studies are necessary, especially concerning the profile of bioactive compounds, since it is a much appreciated beverage in several regions in Brazil

Actividad antioxidante en vinos de jabuticaba:comparación con vinos de uva. Muestras de vinostintos y blancos secos de jabuticaba y uva fueronanalizadas con la finalidad de verificar la actividad antioxidante de los compuestos presentes. Fue utilizado el sistema ?-caroteno/ácido linoleico, en el cual fueron hechas medidasespectrofotométricas de volúmenes de 20?L,50?L, 100?L y 200?L de muestras en duplicadocon lecturas a cada 15 minutos durante doshoras. Fue verificado que el vino tinto de jabuticaba presentó mejor actividad antioxidante con una inhibición de la oxidación próxima a los valores del BHT (antioxidante sintéticoutilizado en la industria alimenticia), inhibiciónen torno de 65%. El estudio cinético reveló quevinos de jabuticaba son eficientes comosecuestradores de radicales libres con F<1 en lafase 1 de la cadena oxidativa, y con resultadossuperiores a los vinos de uvas. El vino dejabuticaba es una buena fuente de antioxidantes,pero son necesarios más estudios en relación aeste, especialmente debido a ser de producciónnacional y a la ausencia de datos sobre el perfilde los compuestos activos de esta bebida muy apreciada en varias regiones del Brasil.

Amostras de vinhos tintos e brancos secos dejabuticaba e uva foram analisadas com afinalidade de verificar a atividade antioxidantedos compostos presentes. Foi utilizado o sistema?-caroteno/ácido linoleico, no qual foram feitasmedidas espectrofotométricas dos volumes de 20?L, 50?L, 100?L e 200?L das amostras em duplicata com leituras a cada 15 minutos porduas horas. Foi verificado que o vinho tinto dajabuticaba apresentou melhor atividade antioxidante e que o mesmo obteve valores deinibição da oxidação próximos ao do BHT (antioxidante sintético utilizado na indústriaalimentícia), com inibição em torno de 65%. No estudo cinético, foi revelado que vinhos de jabuticaba são bons sequestradores de radicais livres com F<1 na fase 1 da cadeia oxidativa ecom resultados superiores aos vinhos de uvas.O vinho de jabuticaba é uma boa fonte de antioxidantes, no entanto, faz-se necessáriomais estudos e em especial sobre perfil de compostos bioativos por se tratar de uma bebida muito apreciada em várias regiões do Brasil.

Atmosfera modificada e condições de armazenamento nas características físico-químicas de jabuticabas da cultivar 'paulista' / Modified atmosphere and storage conditions in jaboticabas cv 'paulista' physicochemistry characteristics

O objetivo deste trabalho foi avaliar o efeito de diferentes embalagens nas características físico-químicas de jabuticabas da cultivar 'paulista', sob condições de temperatura ambiente (25°C) e refrigeração (8°C). Os frutos foram analisados no início do experimento (tempo 0) e no 2°, no 4°, no 6° e no 8° dia de armazenamento, em temperatura ambiente, e no 3°, no 6°, no 9° e no 12° dia, em câmara de refrigeração, sob diferentes condições de recobrimento (controle, filme PVC, plástico de polietileno e fécula de araruta a 2 por cento gelatinizada). Estes foram avaliados quanto à perda de massa fresca, pH, acidez titulável, teor de sólidos solúveis (°Brix), teor de ácido ascórbico e teor de açúcares redutores. O experimento foi realizado em delineamento inteiramente casualizado, em fatorial 2 x 4 x 4, com três repetições. A embalagem de PVC e polietileno demonstraram ser eficientes em reduzir as perdas de massa em ambas as condições de temperatura. A refrigeração foi importante para a manutenção pós-colheita da jabuticaba, pois manteve a qualidade dos frutos armazenados até 12 dias, em comparação com os frutos armazenados em temperatura ambiente, por oito dias. A interação tempo e embalagem foi significativa para os parâmetros perda de massa, pH, teor de sólidos solúveis, vitamina C e açúcares redutores, exceto para acidez. No entanto, não se pode afirmar, com base nos resultados das análises químicas, qual dos tipos de embalagem proporcionou melhores condições de conservação.

The aim of this study was to evaluate the effect of different kinds of packing in araruta and jaboticabas cv paulista' physicochemistry characteristics, stored at room temperature (25°C) and at refrigeration (8°C). The fruits were analyzed at the beginning of experiment (time 0) and in the 2nd, 4th, 6th and 8th days of storage at room temperature, and in the 3rd, 6th, 9th and 12nd days in the refrigeration camera under different cover conditions (control, PVC film, polyethylene plastic and araruta starch to 2 percent jellied). They were evaluated by the loss of fresh mass, pH, titrable acidity, soluble solids content (°Bx), ascorbic acid content and reducing sugar content. The experiment was carried out by completely randomized design in factorial 2 x 4 x 4 with three repetitions. The PVC film and polyethylene proved to be efficient in reducing the mass losses in both temperature conditions. The refrigeration was important for the post-harvest maintenance of jabuticaba, because it maintained the superior quality of fruits stored up to 12 days compared to the stored at room temperature for 8 days. The interaction time and packing was significant for most of the evaluated parameters, except for the acidity one. However it cannot be affirmed through the results of the chemical analyses which of the different types of packing tested showed better conservation conditions.

Eficácia do extrato de Myrciaria cauliflora (Mart. ) O. Berg. (jabuticabeira) sobre bactérias orais / Effectiveness of the Myrciaria cauliflora (Mart. ) O. Berg. extract on oral bacteria

O objetivo deste estudo foi avaliar a atividade antimicrobiana do extrato da folha de Myrciaria cauliflora (Mart.) O. Berg., Myrtaceae (jabuticabeira) sobre: Streptococcus mitis (ATCC 903), Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 15300), Streptococcus oralis (ATCC 10557), Streptococcus salivarius (ATCC 7073) e Lactobacillus casei (ATCC 9595). A pesquisa foi realizada através de técnicas bacteriológicas laboriosas. Os ensaios foram realizados pelo método da diluição em meio sólido para a determinação da Concentração Inibitória Mínima (CIM). Os resultados obtidos foram transferidos para um banco de dados informatizado e calculados os parâmetros estatísticos mediante o emprego do programa SPSS versão 13.0. Utilizou-se, ao nível de 5 por cento de significância, o teste t-Student. Em estudo comparativo, foi determinada a CIM do digluconato de clorexidina a 0,12 por cento. O extrato de jabuticabeira formou halos de inibição variando de 10 a 18 mm de diâmetro e apresentou desempenho médio significativamente inferior em relação a clorexidina, na comparação do extrato bruto vs substância pura e nas concentrações 1:2 e 1:4. Conclui-se, que o extrato de Myrciaria cauliflora produziu uma significante atividade bacteriostática in vitro sobre as bactérias do biofilme dental, o que sugere a utilização dessa substância como meio alternativo e economicamente viável para o controle de afecções em Odontologia.

The aim of this study was to evaluate in vitro antimicrobial activity of Myrciaria cauliflora (Mart.) O. Berg., Myrtaceae leaves extract on: Streptococcus mitis (ATCC 903), Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 15300), Streptococcus oralis (ATCC 10557), Streptococcus salivarius (ATCC 7073) and Lactobacillus casei (ATCC 9595). The study was done using laborious bacteriological techniques. The assays were made through dilution in agar diffusion method in order to reach the Minimum Inhibitory Concentration (MIC) determination. The results obtained were transferred for database and had the statistics parameter calculated by SPSS program 13.0 version. The t-Student test was used with 5 percent of significance. In comparative study, the digluconate of chlorexidine (0,12 percent) was determinated. The leaf extract of Myrciaria cauliflora Berg. formed halos of inhibition oscillating between 10-18 mm of diameter and it showed middle performance significantly inferior in respect to digluconate of chlorexidine (0,12 percent), on the pure extract and in the concentrations 1:2 and 1:4. It follows that, the Myrciaria cauliflora Berg. extract produced a significative in vitro bactericidal activity on the former oral biofilm bacterias, that suggests the use of these substances as an economic and viable kind of alternative to the control of odontological diseases.

Caracterização química do fruto jabuticaba (Myrciaria cauliflora Berg) e de suas frações / Chemical characterization of the jabuticaba fruits (Myrciaria cauliflora Berg) and their fractions

A jabuticaba (Myrciaria cauliflora Berg) é uma fruta nativa brasileira e pouco se conhece sobre os constituintes químicos nas diversas partes do fruto, principalmente, em relação aos compostos bioativos. Este trabalho teve por objetivo determinar a composição centesimal e alguns compostos bioativos no fruto inteiro e nas frações da jabuticaba de duas variedades (Paulista e Sabará). O experimento foi conduzido em delineamento inteiramente casualizado em esquema fatorial 4 x 2 x 3, sendo 4 fatores, fruto inteiro, casca, polpa e semente; 2 variedades de jabuticabas e 3 repetições. Os frutos foram colhidos, selecionados, pesados, sanitizados, separados em fruto inteiro, casca, polpa e semente, que foram triturados, congelados e liofilizados até peso constante. Os teores de proteína bruta e extrato etéreo foram baixos. Os teores de cinzas não variaram entre as frações, exceto para as cascas da variedade Sabará que apresentaram os maiores teores. As fibras alimentares apresentaram diferença significativa entre as frações, não variando entre as duas variedades. As cascas apresentaram os maiores teores: 33,80 g/100g na Paulista e 33,23 g/100g na Sabará, sendo o maior percentual de fibras insolúveis. O maior teor de extrato não nitrogenado foi encontrado na polpa e o menor na casca, não se diferenciando entre as variedades. Quanto aos compostos bioativos, foram analisados saponinas, ácido oxálico, inibidor de tripsina, polifenóis e lectinas. Destes apenas os polifenóis nas frações casca apresentaram níveis elevados, necessitando de caracterizá-los, a fim de se verificar se o fruto é seguro para ser usado na indústria alimentícia.

Jabuticaba, Myrciaria cauliflora Berg, is a native Brazilian fruit; very little is known about the chemistry of its components, specially the bioactives compounds. The purpose of this paper was to determine the centesimal composition and some bioactives compounds of the whole fruit and fractions of two varieties of jabuticaba (Paulista and Sabará). The experiment was conducted in a completely randomized design on a 4 x 2 x 3 factorial scheme (4 factors: whole fruit, skin, pulp and seed; 2 varieties: Paulista e Sabará, and 3 replicates). The fruits were sampled, selected, weighted, sanitized and fractionated into whole fruit, skin, pulp and seed that were grinded, frozen and lyophilized to a constant weight. Protein contents and of ether extract were low for all fractions. The ash contents indicate elevated levels of minerals, specially on the Sabará variety. The content of alimentary fibers differed widely among fractions, with no differences between varieties. The skin fractions presented the highest levels of fiber, 33.80 g/100g on Paulista and 33.23 g/100g on Sabará, most of it was insoluble fibers. The greatest amount of non nitrogen extract was found in the pulp fraction, while the lowest was found in the skin, with no differences between varieties. Regarding bioactives compounds, only polyphenols were presented at considerable levels in all the fractions but the pulp one, showing the need for better characterization before using the fruit in the food industry.