RESUMO
Objective To investigate the effect of the small dose dopamine on the surgical rate, curative effect and adverse reactions of the neonatal necrotic enteritis. Methods From January 2013 to January 2017 in our hospital for treatment of 60 cases of neonatal necrotizing enterocolitis were as the research object, and to give a randomized, control group 30 cases, give simple phentolamine static drop treatment; Intervention group, 30 cases in the control group on the basis of small dose dopamine was given to two groups of children with fasting time, length of hospital stay, operation rate, efficacy and adverse reactions happen to comprehensive evaluation. Results The average fasting time and hospital length of the intervention group were significantly better than the control group (P< 0.05), which was statistically significant. Intervention group with operation rate and total effective rate were 3.3％, 93.3％, the control rate of surgery, treatment in children with total effective rate of 16.7％, 70.0％ respectively, there are statistically significant difference (P< 0.05); There were only two cases of adverse reaction in the intervention group, 6.7％, and the difference of 23.3％ in the control group was statistically significant (P< 0.05). Conclusion On the basis of phentolamine treatment of neonatal necrotizing enterocolitis with small dose of dopamine was given to shortening the time of fasting and hospitalization time, decrease the rate of transit operation, and less adverse reaction, curative effect is distinct, worthy of clinical popularization and application.
RESUMO
Samples of intestine with necrotic enteritis from 63 pigs naturally infected with porcine circovirus type 2 (PCV2) were studied. Colon was the main target of PCV2 associated necrotic enteritis in 60 cases. Immunohistological investigations were carried out to detect the presence of PCV2 in necrotic lesions and to identify the type of cells infected by the virus. Crypt epithelial cells had positive labelling for PCV2 in 17 cases. Depletion of goblet cells occurred in 10 cases. In 24 necrotic enteritis cases, co-infection of PCV2 and Salmonella was identified. An increased rate of apoptosis in the crypt epithelial cells of the large intestine from PCV2 of naturally infected pigs was observed. Immunohistochemical findings confirmed the presence of PCV2 within cells from necrotic intestinal tissue, suggesting that PCV2 may play a role in the development of those lesions. Diagnosis of necrotic enteritis associated with PCV2 should be based on the detection of PCV2 antigen or DNA in the necrotizing lesions. However, bacteriological examination should be performed to rule out the presence of bacterial agents, since co-infections are likely to occur in PCV2 affected pigs.
Foram selecionadas amostras intestinais com enterite necrótica de 63 suínos naturalmente infectados pelo circovírus suíno tipo 2 (PCV2). Enterite necrótica associada com PCV2 ocorreu principalmente no cólon, em 60 casos. Análise imuno-histoquímica foi realizada para identificar a presença de PCV2 em lesões necróticas e o tipo de células infectadas pelo vírus. Células epiteliais das criptas apresentaram marcação positiva para PCV2 em 17 casos. Depleção de células caliciformes ocorreu em 10 casos. Em 24 casos de enterite necrótica, observou-se co-infecção por PCV2 e Salmonella. Foi observado um aumento no índice de apoptose nas células das criptas do intestino grosso de suínos naturalmente infectados com PCV2. Os achados imuno-histoquímicos e histopatológicos sugerem que a infecção por PCV2 das células do tecido intestinal pode ocasionar enterite necrótica. O diagnóstico de enterite necrótica associada com PCV2 deve ser baseado na detecção do antígeno ou do DNA viral nas lesões necróticas. Contudo, análise bacteriológica deve ser realizada para descartar a presença de agente bacteriano, já que co-infecções são comuns.
