RESUMO
A survey of nematodes associated with the red palm weevil Rhynchophorus ferrugineus was conducted in southern Italy in 2015 and 2016 in order to create a species inventory and obtain data about nematode biodiversity. A total of 70 insect samples (pupae and adults) were collected from infested Phoenix canariensis, Phoenix dactylifera, and Chamaerops humilis palms in three Italian Regions: sampling took place at 11 locations in Apulia, 1 in Basilicata, and 1 in Sardinia regions. Individual insects were dissected to determine nematode presence, and different nematode species were also recovered from red palm weevil cocoons collected at the sites in Apulia. Individual nematodes were molecularly identified by sequencing the ITS, D2-D3 expansion domains of the 28SrRNA gene and the mitochondrial COI and inferring the phylogenetic relationships. The insect-associated nematofauna identified belonged to the families Rhabditidae, Cephalobidae, and Diplogastridae. Just two nematode species, Teratorhabditis synpapillata and Mononchoides macrospiculum, were always found in association with adult insects and cocoons taken from all sampling sites. This paper reports on the biodiversity of the nematodes associated with R. ferrugineus and on current knowledge of the specific habitat of specialized and divergent entomophilic nematodes.
RESUMO
ABSTRACT: The aim of this study was to conduct a survey on nematodes associated with five cultivated fruit trees growing in the Agroforestry System (AFS) of the municipality of Oiapoque, Amapá, Brazil. Rhizosphere samples were collected from three points at the base of murici ( Byrsonima sp.), soursop ( Annona muricata ), cupuaçu ( Theobroma grandiflorum ), passion fruit ( Passiflora sp.) and peach palm ( Bactris gasipaes ) trees. Four species of phytonematodes were identified, namely Pratylenchus brachyurus, Helicotylenchus dihystera, Mesocriconema xenoplax and Rotylenchulus reniformis . The most prevalent and abundant species was R. reniformis , which was found in the rhizospheres of passion fruit, cupuaçu, soursop, and peach palm. The first record in Brazil of the nematode P. brachyurus , found in the roots of murici is reported here, and all of the nematode species identified here are the first records for fruit trees in the state of Amapá.
RESUMO: Este estudo teve como objetivo o levantamento de nematoides, associados a cinco fruteiras cultivadas em Sistema Agroflorestal (SAF), no município de Oiapoque, AP. Amostras da rizosfera foram coletadas em três pontos, na base de plantas de murici ( Byrsonima sp.), graviola ( Annona muricata ), cupuaçu ( Theobroma grandiflorum ), maracujá ( Passiflora sp.) e pupunha ( Bactris gasipaes ). Foram constatadas quatro espécies de fitonematoides: Pratylenchus brachyurus, Helicotylenchus dihystera, Mesocriconema xenoplax e Rotylenchulus reniformis . A espécie mais prevalente e abundante foi R . reniformis , encontrado na rizosfera de maracujá, cupuaçu, graviola e pupunha. Foi relatado o primeiro registro de P. brachyurus em raízes de muricizeiro no Brasil e todas as espécies encontradas configuram-se como primeiros registros de fitonematoides em fruteiras no estado do Amapá.
