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Objective To compare the diagnostic performance of quantitative and semi-quantitative hemodynamic parameters of breast dynamic contrast-enhanced MRI (DCE-MRI) in differential diagnosis of benign and malignant breast lesions. Methods Fifty-nine patients (66 lesions) with breast lesions underwent hybrid DCE MRI with high temporal and spatial resolution, and semi-quantitative and quantitative hemodynamic parameters were obtained. The semi-quantitative parameters included time-intensity curve (TIC), initial area under the gadolinium curve (IAUGC), maximum slope of increase (MaxSlope), contrast enhancement rate (CER) and positive enhancement integral (PEI), while quantitative parameters included volume transfer constant (Ktrans), rate constant (Kep) and extravascular extracellular volume fraction (Ve). The differences of all the parameters between benign and malignant breast lesions were compared by using non-parametric tests. ROC was used to analyze the diagnostic performance. Results There were 31 malignant lesions (malignant group) and 35 benign ones (benign group). There were significant differences of Ktrans, Kep, TIC, IAUGC and MaxSlope between the two groups (all P0.05). AUC of ROC curve of Ktrans, Kep, TIC, IAUGC and MaxSlope were all >0.7. AUC of semi-quantitative parameter combination in diagnosis of benign and malignant breast lesions were significantly higher than those of single parameter (all P<0.05), AUC of quantitative parameter in combination was not significantly higher than those of Ktrans (P=0.134), but significantly higher than those of Kep and Ve (all P<0 001). There was no significant difference in AUC between combined semi-quantitative and quantitative parameters in diagnosis of benign and malignant breast lesions (P=0.614). Conclusion Ktrans, Kep, TIC, IAUGC and MaxSlope have good diagnostic performance in differentiating benign and malignant breast lesions. The combined semi-quantitative and quantitative hemodynamic DCE-MRI parameters have similar diagnostic performance.
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Objective To investigate the antitumor effect of specific cytotoxic T lymphocytes induced by renal cell carcinoma multiple antigen peptide dendritic cell (DC) vaccine in vitro. Methods Dendritic cells were induced by hGM-CSF,hIL-4 from blood.Peptide of renal cell carcinoma cell line (RCC786-0)was got by citrate-phosphate buffer elution.Peripheral blood mononuclear cell was cultured.Multiple antigen peptide DC cell vaccine was obtained by acid-eluted peptide pulsed DC.The tumor antigen specific CTL was generated from activated T cell by vaccine.Killing activity of the tumor antigen specific CTL was activated by vaccine. Results Antigen peptide DC cell vaccine could show a strong cytotoxic activity of CTLs(31.93±5.05%),which was much higher than control groups(5.88±2.26%,8.03±6.70%,9.70±2.09%,9.35±3.58%). Conclusion Renal cell carcinoma antigen peptide DC vaccine could show a high antitumor effect in vitro.
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Glutathione S-transferases(GSTs) are a family of soluble detoxification enzymes which use reduced glutathione(GSH) in conjugation and reduction reactions.Toxic electrophiles,including a variety of carcinogens,are substrates for the GSTs and are more easily excreted into bile or urine after conjugation or reduction .The three-dimensional structures of GSTs from several species,including humans,have been determined.GST activity has been found to present in all human tissues,and expression of the various GST isoenzymes differs in degree in the various tissues. Glutathione S-transferases may play a role in the protection of cells against toxic electrophiles and in the resistance to cancer chemotherapeutic agants.The polymorphisms of GSTs genes are one of the important factors that exercise influence on individual susceptibility to cancer.
