Two new acetoisovanillone glycosides from the water-soluble fraction of Paeonia ostii.

The cortex root of Paeonia ostii, is used as a traditional Chinese medicine for treating female diseases. Phytochemical investigation of the water-soluble fraction of the plant led to the isolation of two new acetoisovanillone glycosides: acetoisovanillone-3-O-ß-D-glucopyranoside (1) and 2-hydroxy-acetoisovanillone-3-O-ß-D-glucopyranoside (2). Their structures were elucidated by extensive spectroscopic methods.

Interactions between heavy metals and other mineral elements from soil to medicinal plant Fengdan (Paeonia ostii) in a copper mining area, China.

In order to analyze the interaction relationship between heavy metals and other mineral elements during the processes of absorption and translocation by plant grown on heavy metal-contaminated area, the concentrations of Cu, Zn, Mn, Cd, Pb, Ca, Mg, Fe, and K in the medicinal plant Paeonia ostii T. Hong et J. X. Zhang and its rhizospheric soil were determined, which grow around an abandoned copper tailings reservoir in Tongling City, China. Geo-accumulation index (Igeo) calculation indicated that Cu and Pb are the main pollution elements in the rhizospheric soil. Moreover, the Cu and Pb concentrations in the cortex moutan of P. ostii exceeded the maximum permissible limits for food product safety. The bioaccumulation factor values of the tested metals in plant roots were found < 0.50, with the exception of Ca (maximum 5.99). The translocation factor values of detected heavy metals Cd and Pb were more than 1.00, which indicated that P. ostii could be considered a potential accumulator plant for Cd and Pb. Significant positive correlations including Cu-Cd, Cu-Zn, Cu-Pb, Cd-Zn, Cd-Fe, Cd-Fe, Zn-Pb, Pb-Fe, Mn-Fe, and Ca-Mg in the cortex moutan and Cu-Zn, Cu-Fe, Zn-Mg, Zn-Fe, and Mn-K in the leaves were observed (P < 0.05). Significant positive correlation between Cu, Zn, Mg, and Fe was also confirmed in the processes of absorption and translocation from the soil to plant (P < 0.05), which evidenced that synergistic element interactions of the essential elements Cu, Zn, Mg, and Fe are a result of the similarity in their ionic radii and octahedral coordination geometry.

Secondary metabolites from endophytic fungus Fusarium oxysporum isolated from Paeonia ostii / 中草药

Objective To study the secondary metabolites of endophytic fungus Fusarium oxysporum form Paeonia ostii. Methods The fermentation liquor of the fungal strain F. oxysporum were isolated and purified using various chromatographic methods. The structures of the compounds were identified by spectrum analysis. Results Twenty-three compounds were isolated from the fungal and their structures were identified as (2S)-proline (1), (22E,24R)-ergosta-7,22-diene-3β,5α,6β-triol (2), cyclo-(S-Pro-S-Leu) (3), cyclo-(L-Ala-L-Pro) (4), cyclo-(Val-Pro) (5), cyclo-(R-Pro-S-Phe) (6), cyclo-(D-cis-Hyp-L-Phe) (7), cyclo-(trans-4-hydroxy- L-Pro-L-Phe) (8), cyclo-(Gla-Tyr) (9), cyclo-(trans-4-hydroxy-L-Pro-L-Leu) (10), L,L-cyclo-(Trp-Pro) (11), cyclo-(L-Leu-Gly) (12), N-(3-(1H-indol-3-yl)propyl)acetamide (13), indole-3-acetic acid (14), 2-piperidone (15), 2-pyrrolidinone (16), thymidine (17), (5S)-5-[(4-hydroxyphenyl)methyl]-2,4-imidazolidinedione (18), (3S,6S)-3-(butan-2-yl)-6-(1-hydroxyethyl) piperazine-2,5-dione (19), cyclo-(Ala-Leu) (20), cyclo-(S-Pro-S-Phe) (21), cyclo-(Tyr-Pro) (22), and cyclo-(L-Phe-L-Tyr) (23). Conclusion With the exception of compounds 2, 6, and 14, the other compounds are isolated from the fermentation liquor of the fungal strain F. oxysporum for the first time.

Chemical constituents from seed cake of Paeonia ostii / 中草药

Objective: To study the chemical constituents from the seed cake of Paeonia ostii. Methods: Chemical constituents were isolated by Sephadex LH-20, preparative HPLC, and silica gel column chromatographies. The structures were elucidated on the basis of spectral data analysis. Results: Five compounds, 1-(p-hydroxybenzoate)-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside (1), polydatin (2), luteolin-7-O-β-D-glucoside (3), oleanolic acid (4), and betulinic acid (5) were isolated from the seed cake of P. ostii. Conclusion: Compound 1 is a new one named as paeoniostin A, compounds 2 and 3 are isolated from the family of Paeoniaceae for the first time, and compounds 4 and 5 are isolated from the plant for the first time.