Effects of pasture management and supplementation on the productive performance of recently lambed ewes.

The hypothesis was tested that the performance of lactating ewes is affected by the supplementation level and pasture management. Two supplementation levels (0.5 and 1.0% of body weight, BW) and two pasture managements (mowed and non-mowed) were tested. Forty adult ewes (2 years old) with an average weight at lambing of 62.97 ± 7.0 kg (day 0) and an average the body condition score of 2.5 points (day 0) were evaluated. Verminosis was monitored with periodic deworming. The number of eggs per gram of feces (EPG) of ewes at lambing was used as a covariate for performance assessments. Ewes lost an average of 7.5 kg over the 90 days post-partum (12% BW). The weaning rate was 53%. The body condition of the ewes was influenced by the post-partum period. The ewes mobilized their body reserves in the first 30 days of lactation. Mowing management negatively affected the nutritive value of the forage. Supplementation with 0.5% BW was sufficient for nutritional management post-partum. Pasture management (mowed vs. not mowed) cannot prevent post-partum weight loss. Supplementation levels and pasture management altered the morphological and chemical components of the pasture.

Temporal Stability of Management Zone Patterns: Case Study with Contact and Non-Contact Soil Electrical Conductivity Sensors in Dryland Pastures.

Precision agriculture (PA) intends to validate technological tools that capture soil and crop spatial variability, which constitute the basis for the establishment of differentiated management zones (MZs). Soil apparent electrical conductivity (ECa) sensors are commonly used to survey soil spatial variability. It is essential for surveys to have temporal stability to ensure correct medium- and long-term decisions. The aim of this study was to assess the temporal stability of MZ patterns using different types of ECa sensors, namely an ECa contact-type sensor (Veris 2000 XA, Veris Technologies, Salina, KS, USA) and an electromagnetic induction sensor (EM-38, Geonics Ltd., Mississauga, ON, Canada). These sensors were used in four fields of dryland pastures in the Alentejo region of Portugal. The first survey was carried out in October 2018, and the second was carried out in September 2020. Data processing involved synchronizing the geographic coordinates obtained using the two types of sensors in each location and establishing MZs based on a geostatistical analysis of elevation and ECa data. Although the basic technologies have different principles (contact versus non-contact sensors), the surveys were carried out at different soil moisture conditions and were temporarily separated (about 2 years); the ECa measurements showed statistically significant correlations in all experimental fields (correlation coefficients between 0.449 and 0.618), which were reflected in the spatially stable patterns of the MZ maps (averaging 52% of the total area across the four experimental fields). These results provide perspectives for future developments, which will need to occur in the creation of algorithms that allow the spatial variability and temporal stability of ECa to be validated through smart soil sampling and analysis to generate recommendations for sustained soil amendment or fertilization.

Effects of grazing on soil properties in mediterranean forests (Central-Eastern Spain).

Traditional management practices, such as grazing, can have adverse impact on soils. Despite an extensive body of literature exploring the effects of grazing on soil and plants worldwide, there is a notable lack of research on its impacts in Mediterranean forests within the Iberian Peninsula Furthermore, there is a knowledge gap on the enzymatic activities and basal respiration of soil in forest after grazing. To address these gaps, this study aimed to investigate the impact of grazing on various important physicochemical and biological soil properties along with vegetation richness in a Mediterranean forest located in Castilla-La Mancha (Central Eastern Spain). Relative to undisturbed sites, grazing significantly reduced soil water content (-53%) and available water (-59%). However, soil hydraulic conductivity remained unaffected by animal trampling and the soil water repellency observed in ungrazed sites disappeared. Grazed soils experienced a slight increase in pH (+18%). Among the biochemical properties studied, only dehydrogenase showed a significant increase (+100%) while basal respiration exhibited a notable decrease (-24%). Grazing resulted in a reduction of plant species richness (-34%) indicating a loss of biodiversity in grazed areas. The observed significant alterations in key soil and plant properties due to livestock activity suggest that grazing has the potential to modify the overall soil quality of these sites. Certain variables that exhibited noteworthy differences between grazed and ungrazed sites could serve as indicators of grazing impacts in Mediterranean forests. These indicators may be considered proxies for establishing effective land management strategies to mitigate degradation in the Mediterranean forest ecosystem.

Discovery of insecticide resistance in field-collected populations of the aphid pest, Acyrthosiphon kondoi Shinji.

BACKGROUND: The bluegreen aphid (Acyrthosiphon kondoi) is a worldwide pest of alfalfa, pulses, and other legume crops. An overreliance on insecticides to control A. kondoi has potentially placed populations under selection pressure favouring resistant phenotypes, but to date, there have been no documented cases of insecticide resistance. Recently, Australian growers began reporting that conventional insecticides were failing to adequately control A. kondoi populations, prompting this laboratory-based investigation into whether these populations have evolved resistance. RESULTS: We discovered four A. kondoi populations with moderate resistance (10-40-fold) to three different insecticide groups: organophosphates, carbamates and pyrethroids. However, A. kondoi populations showed no resistance to the butenolide, flupyradifurone. We were unable to identify general metabolic mechanisms using synergist assays (cytochromes P450, glutathione S-transferases, or esterases), indicating that further detailed molecular investigations to characterise the putative resistance mechanism are needed. CONCLUSION: Insecticide-resistant A. kondoi present an emerging challenge to Australian agriculture. Growers require new tools and updated strategies, including access to newer chemistries, to alleviate their reliance on the few insecticides currently registered against A. kondoi. The implications of insecticide resistant A. kondoi for future management, the potential mechanisms of resistance, and future research priorities are discussed. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Powder and agglomerated free-choice minerals for grazing cattle: animal responses and chemical and physical alterations of the mineral mixture.

The aim was to evaluate the animal response and the chemical and physical changes of free-choice mineral mixtures fed to grazing cattle. Growing beef cattle were fed either powder (POW) or agglomerated (AGL) mineral mixtures in three different experiments (Exp.), carried out in pastures of Brachiaria grass. In Exp. 1 and 2, the mineral mixtures were disposed in unsheltered troughs (POWun vs. AGLun), being delivered once (D0, Exp.1) or twice (D0 and D8, Exp. 2), throughout 14-day periods. In Exp. 3, POWun and AGLun were additionally compared to POW in sheltered troughs (POWshe), and the mineral mixtures were disposed in D0, throughout 21-day periods. Non-consumed supplement was removed and sampled on D14 (Exp. 1 and 2) or D21 (Exp. 3). Evaluations included average daily body weight gain (ADG), daily disappearance of the supplement (DSD), penetration force of the supplement mass, faecal chemical composition and serum levels of Ca, P and Mg. In Exp. 1, no effects were observed on ADG and faecal mineral concentrations, however, changes in mineral concentrations and a 40% reduction in Na concentration in the supplement were observed, compared to the initial concentration. AGLun had a lower penetration force. In Exp. 2, there were no effects on DSD and faecal mineral concentrations. POWun showed a smaller reduction in Na content compared to AGLun, and AGLun showed lower penetration force. In Exp. 3, the treatments did not affect ADG, but there was a trend towards higher DSD and serum phosphorus (P) concentration for AGLun (p = 0.08). Higher faecal Na concentration was observed for AGLun and higher Na concentration occurred in non-consumed mixture of POWshe. Mineral supplements offered in uncovered troughs showed altered chemical and physical characteristics, with possible effects on supplement intake. However, the general changes are unlikely to alter animal performance.

Feed resources used for small ruminant nutrition in Sub-Saharan Africa: a case study of Uganda.

Small ruminants are of great importance to the livelihoods of many people in Sub-Saharan Africa (SSA) since they act as a source of meat, income, hides and skins and for cultural identity. Despite their great importance, especially to rural smallholder farmers, their production in Africa is hindered by poor nutrition. The first stage of establishing development strategies to increase feed quality and quantity is to conduct a comprehensive inventory of the feed resources that are currently available, especially in SSA. Therefore, this study aims to evaluate the availability and nature of the common feed resources that are used in the feeding of small ruminants in Uganda. This study involved a comprehensive review of various scientific, technical and economic literature that has been published up to date. Our results show that the available feed resources in Uganda can be grouped mainly into:- crop wastes and residues, agro-industrial by-products, conserved feeds, natural pastures and browse. The main feed resources for small ruminants in Uganda have been identified as natural pastures, which are generally characterised by a low nutritive value. However, browses are seen to have a high crude protein content and could be used as a supplement to grass forages and crop residues. It has been noted that farmers lack knowledge related to feed processing and management, in addition to a lack of policy governing feed quality. Therefore, there is a need to train farmers on how to process and utilise crop residues, in addition to proper feed management practices like pasture cultivation and conservation. It is also necessary to introduce widely new pasture species with better nutritional values that are suitable for the local production systems.

Plant biomass and seed production of the legumes Aeschynomeme histrix and Stylosanthes hamata and the potential of endozoochory by cattle and sheep in semi-arid native pastures.

Endozoochory is a substantial vector for seed dispersal and plays an important role in vegetation dynamics, mainly in colonisation processes through seed input to the vegetation and soil seed bank. We investigated the endozoochorous seed input by cattle and sheep on a pasture located in the western region of Burkina Faso. Through germination experiments, we assessed viable seed content of the dung of these grazing animals to estimate their suitability and efficiency for seed dispersal of fodder legumes. Cattle and sheep were daily fed seeds of Sthylosanthes hamata and Aeschynomene histrix, mixed with cotton seed cake. Faeces containing seeds of both legumes were collected 24 h after feeding. One part of faeces samples was spread in buckets of soil for direct germination in the greenhouse to evaluate germinating seed content. To improve pastures, a randomized completed design with 6 replications was conducted with both legumes and phosphorus fertilization (0 and 100 kg/ha of P2O5) and year as experimental factors. Recovery of A. histrix seeds was better than that of S. hamata with cattle (18 and 9%, respectively) compared to sheep. Seed recovered from faeces had higher germination with sheep than cattle. Thus, S. hamata seed recovered from faeces germinated well (12 and 45% with cattle and sheep, respectively, than fresh seeds used as control. However, A. histrix's seeds recovered from faeces germinated less than control (P < 0.001). The findings confirmed that ruminants could be used for targeted legume seed dispersal in natural pastures. A. histrix and S. hamata have high potential for plant biomass and seed production when phosphorus is applied. Seed ingestion by ruminants should be undertaken for improving natural pastures in semi-arid zones as lower cost practice.

Grazing management and stocking strategy decisions for pasture-based beef systems: experimental confirmation vs. testimonials and perceptions.

Grazing management and stocking strategy decisions involve the manipulation of grazing intensity, grazing frequency, and timing of grazing to meet specific objectives for pasture sustainability and economic livestock production. Although there are numerous stocking systems used by stakeholders, these methods may be broadly categorized as either continuous or some form of rotational stocking. In approximately 30 published experiments comparing continuous vs. rotational stocking, there was no difference in liveweight gain per animal between stocking methods in 66% of studies. There was no difference in gain per hectare between methods in 69% of studies, although for gain per hectare the choice of fixed or variable stocking rate methodology affected the proportion (92% for fixed; 50% for variable). Despite these experimental results showing limited instances of difference between rotational and continuous stocking, rotational strategies (e.g., "mob stocking" or "regenerative grazing") have received what appears to be unmerited acclaim for use for livestock production. Many proposed "mob stocking" or "regenerative grazing" systems are based on philosophies similar to high intensity-low frequency stocking, including provision for >60 d of rest period from grazing. In addition, grazing management practitioners and stakeholders have voiced and proposed major positive benefits from rotational stocking, "mob stocking", or "regenerative grazing" for soil health attributes, carbon sequestration, and ecosystem services, without experimental evidence. The perceptions and testimonials supporting undefined stocking systems and methods have potential to mislead practitioners and result in economic disservices. Thus, we suggest that scientists, extension-industry professionals, and producers seek replicated experimental data as the basis for predicting outcomes of grazing decisions.

Pasture Minerals for Dairy Cattle.

While mineral requirements do not differ with the production systems, the forage bases of the different dairy production systems influence the risk of mineral deficiency. Testing representative pastures on a farm is a key to understanding the potential for risk of mineral deficiency and should be combined with blood or tissue samples, clinical observation, and responses to treatment to evaluate the need for supplementation.

Shepherds View of Large Carnivore Recovery in the Pyrenees, Spain.

The studied farms are small family businesses, and so, in more than half of the cases, their continuity is not guaranteed. Livestock management is typical of a mountain system, in which the animals graze throughout the year in cultivated fields, sown meadows, forests near the farms, and mountain pastures during the three summer months. The herds always have the constant surveillance of a shepherd. Farmers consider the current infrastructure present in mountain grasslands insufficient to facilitate the management and care of their herd. Their activity conflicts with various species of wildlife, such as the wild boar, Sus scrofa, roe deer, Capreolus capreolus, or griffon vulture, Gyps fulvus, and large carnivores such as the brown bear, Ursus arctos, or the grey wolf Canis lupus, despite all of them taking preventive measures to defend their herds from predators. The most widely used prevention measures are the presence of mastiff dogs, Canis lupus familiaris, next to the herds and the use of electric fencing to lock up livestock at night. Farmers reject the presence of bears and wolves in their area, considering it a real threat to the continuity of their economic activity, which presents a high degree of vulnerability.

Infectious keratoconjunctivitis in semi-domesticated reindeer (Rangifer tarandus tarandus): a questionnaire-based study among reindeer herders in Norway and Sweden.

BACKGROUND: The effects of climate change, loss of pastureland to other land usage and presence of large carnivores are the main reasons for the increase in supplementary feeding of semi-domesticated reindeer (Rangifer tarandus tarandus) in Fennoscandia over the last decades. Feeding might expose reindeer to stress and increased animal-to-animal contact, leading to an increased risk of infectious disease transmission, such as infectious keratoconjunctivitis (IKC). As it can develop rapidly and be very painful, IKC is described as an important animal welfare concern and a potential source of economic loss. The aim of this study was to investigate the current presence of IKC and potential associations between IKC and supplementary feeding through an online questionnaire survey, distributed among reindeer herders in Norway and Sweden in 2021. RESULTS: Seventy-six reindeer herders (33 from Norway and 43 from Sweden) responded to the questionnaire, representing 6% and 4% of the registered reindeer herding groups in Norway and Sweden, respectively. Infectious keratoconjunctivitis was common, with 54 (71%) of the 76 herders that responded having observed clinical signs during the past 10 years. These signs were mainly observed as increased lacrimation, causing "wet cheeks", but also as keratitis and conjunctivitis. Autumn and winter were the seasons in which IKC was observed most. The herders reported several measures, such as slaughter and isolation of affected reindeer, to counteract the spread of disease. The herding year 2019/2020 was associated with reports of outbreaks of IKC in herds as well as being the herding year where most herders (80%) had performed supplementary feeding. A significant association was found between IKC and feeding performed in an enclosure (odds ratio = 15.20), while feeding on free-range areas had a non-significant, negative, relationship with the appearance of IKC outbreaks (odds ratio = 0.29). Finally, there was a trend in the data suggesting that IKC affected calves especially. CONCLUSIONS: Infectious keratoconjunctivitis is a common disease, mainly observed in winter and autumn. It usually has mild to moderately severe clinical signs. Our results imply that IKC is associated with stress and feeding situations and that calves might be more susceptible than adults, however, this needs to be confirmed with further studies, preferably at an individual animal level.

Exploring the diversity and composition of soil microbial communities in different soybean-maize management systems.

Soybean-maize are cultivated in different management systems, such as no-tillage and pastures, which presents potential to add organic residues, and it can potentially impacts the soil microbial community present in these systems. Thus, this study aimed to examine the effects of different soybean-maize management practices on the diversity and composition of soil microbial communities. Specifically, 16 S rRNA amplicon sequencing was used to investigate whether the use of pasture species in a fallowing system influences microbial communities in a soybean-maize rotation system, as compared to conventional tillage and no-tillage systems. The results indicate that the inclusion of the pasture species Urochloa brizantha in soybean-maize management systems leads to distinct responses within the soil microbial community. It was found that different soybean-maize management systems, particularly those with U. brizantha, affected the microbial community, likely due to the management applied to this pasture species. The system with 3 years of fallowing before soybean-maize showed the lowest microbial richness (∼2000 operational taxonomic units) and diversity index (∼6.0). Proteobacteria (∼30%), Acidobacteria (∼15%), and Verrucomicrobia (∼10%) were found to be the most abundant phyla in the soil under tropical native vegetation, while soils under cropland had an increased abundance of Firmicutes (∼30% to ∼50%) and Actinobacteria (∼30% to ∼35%). To summarize, this study identified the impacts of various soybean-maize management practices on the soil microbial community and emphasized the advantages of adding U. brizantha as a fallow species.

[Risk factors for infectious foot -disorders on two Swiss cattle -mountain pastures]. / Risikofaktoren für infektiöse Zehen­erkrankungen auf zwei Schweizer ­Rinderalpen.

INTRODUCTION: In the present study, risk groups for infectious foot disorders were identified on two large Swiss cattle mountain pastures by analyzing animal and treatment data of a total of 3256 animals of the bovine species. Both mountain pastures were part of the Federal Office for Agriculture (FOAG) consultancy project «Healthy animals, attractive herdsmen positions and less medication on large cattle mountain pastures¼. The project was launched in 2020 following the increased incidence of lameness on these mountain pastures. Bacteriological and histological analyses were to provide information as to whether the most common foot disorder was interdigital phlegmon (IP) or whether digital dermatitis also occurred. Further, the temporal distribution of cases over the mountain pasture season and the influence of mountain pasture and year were investigated and interpreted for the project years 2020 to 2022, and treatment incidences were compared between years. Multiple treatment cycles in the same individual were classified into persistent infections and new infections. Nineteen of 394 first-treated cattle were clinically examined, 12 of them were additionally sampled for bacteriological and histological analyses. All cases examined showed, both clinically and following laboratory analyses, typical characteristics for IP. In contrast, there was no specific evidence for the presence of digital dermatitis. No persistent infections occurred during treatment with benzylpenicillin. Multivariate logistic regression analyses identified > 365-730-day-old cattle (odds ratio OR 8,29), as well as inseminated (OR 5,30) and non-inseminated (OR 7,85) heifers as risk groups for the disease studied (p < 0,05). Association with the oestrus activity of non-inseminated heifers and a generally higher locomotor activity in heifers compared to cows - with a correspondingly increased risk of injury - is conceivable. Meat breeds had a reduced risk compared to dairy breeds (OR 0,29). Breed differences in behavior and/or the effectiveness of the local immune response might have an impact. Knowing about these risk groups can be put to use in the future when selecting animals to be taken to the mountain pastures and/or when planning pasture management in order to reduce the prevalence of infectious foot disorders and thereby the use of antibiotics.

INTRODUCTION: Dans la présente étude, nous avons identifié, grâce à l'analyse des données concernant les animaux et les traitements d'un total de 3256 animaux de l'espèce bovine, des groupes à risque pour les maladies infectieuses des onglons sur deux grands alpages bovins en Suisse. Les deux alpages faisaient partie du BLW-projet de conseil «Animaux sains, postes de bergers attractifs et moins de médicaments sur les grands alpages bovins¼. Le projet a débuté en 2020 suite à l'accumulation de problèmes de boiteries sur ces alpages. Des analyses bactériologiques et histologiques devaient fournir des indications afin de savoir si la maladie des onglons majoritaire était le phlegmon interdigité (PI) ou si la dermatite digitale était également présente. La répartition temporelle des cas de maladies durant la saison d'estivage et l'influence de l'alpage et de l'année pour les années de projet 2020 à 2022 ont été évaluées et interprétées, et les incidences des traitements comparées entre les années. Les cycles de traitement multiples chez le même individu ont été répartis en deux groupes: les infections persistantes et les nouvelles infections. Dix-neuf des 394 bovins primo-traités ont été évalués cliniquement. Douze de ces 19 animaux ont également été soumis à un échantillonnage bactériologique et histologique. Tous les cas étudiés présentaient, aussi bien cliniquement que suite à l'analyse technique en laboratoire, les caractéristiques typiques d'un PI. Par contre, il n'y a pas eu d'indication quant à la présence de dermatite digitale. Aucune infection persistante n'a été constatée après traitement avec de la Benzylpénicilline. Les bovins âgés > 365­730 jours (OR 8.29), ainsi que les génisses inséminées (odds ratio OR 5.30) et non inséminées (OR 7.85) ont été identifiés, suite à une analyse de régression logistique multivariée, comme faisant partie de groupes à risque pour la maladie étudiée (p < 0.05). Un lien avec l'activité en période de chaleurs des génisses non inséminées et une activité locomotrice généralement plus intensive des génisses par rapport aux vaches ­ avec par conséquent un risque accru de blessures ­ est concevable. Le risque était réduit pour les races à viande par rapport aux races laitières (OR 0.29). Des différences de comportement et/ou d'efficacité de l'immunité locale entre les races pourraient avoir une influence. Le fait de connaître ces groupes à risque pourrait être mis à profit à l'avenir lors de la sélection des animaux à monter à l'alpage et/ou lors de la planification de la gestion des prairies, afin de diminuer la prévalence des maladies infectieuses des onglons et ainsi l'utilisation d'antibiotiques.

Effect of Long-Term Semiarid Pasture Management on Soil Hydraulic and Thermal Properties.

Semiarid pasture management strategies can affect soil hydraulic and thermal properties that determine water fluxes and storage, and heat flow in unsaturated soils. We evaluated long-term (>10 years) perennial and annual semiarid pasture system effects on saturated hydraulic conductivity (ks), soil water retention curves (SWRCs), soil water thresholds (i.e., volumetric water content (θv) at saturation, field capacity (FC), and permanent wilting point (PWP); plant available water (PAW)), thermal conductivity (λ), and diffusivity (Dt) within the 0-20 cm soil depth. Forage systems included: Old World bluestem (Bothriochloa bladhii) + legumes (predominantly alfalfa (Medicago sativa)) (OWB-legume), native grass-mix (native), alfalfa + tall wheatgrass (Thinopyrum ponticum) (alfalfa-TW), and annual grass-mix (annual) pastures on a clay loam soil; and native, teff (Eragrostis tef), OWB-grazed, and OWB-ungrazed pastures on a sandy clay loam soil. The perennial OWB-legume and native pastures had increased soil organic matter (SOM) and reduced bulk density (ρb), improving ks, soil water thresholds, λ, and Dt, compared to annual teff and alfalfa-TW (P < 0.05). Soil λ, but not Dt, increased with increasing θv. Grazed pastures decreased ks and water retention compared to other treatments (P < 0.05), yet did not affect λ and Dt (P > 0.05), likely due to higher ρb and contact between particles. Greater λ and Dt at saturation and PWP in perennial versus annual pastures may be attributed to differing SOM and ρb, and some a priori differences in soil texture. Overall, our results suggest that perennial pasture systems are more beneficial than annual systems for soil water storage and heat movement in semiarid regions.

Local and landscape environmental heterogeneity drive ant community structure in temperate seminatural upland grasslands.

Environmental heterogeneity is an important driver of ecological communities. Here, we assessed the effects of local and landscape spatial environmental heterogeneity on ant community structure in temperate seminatural upland grasslands of Central Germany. We surveyed 33 grassland sites representing a gradient in elevation and landscape composition. Local environmental heterogeneity was measured in terms of variability of temperature and moisture within and between grasslands sites. Grassland management type (pasture vs. meadows) was additionally included as a local environmental heterogeneity measure. The complexity of habitat types in the surroundings of grassland sites was used as a measure of landscape environmental heterogeneity. As descriptors of ant community structure, we considered species composition in terms of nest density, community evenness, and functional response traits. We found that extensively grazed pastures and within-site heterogeneity in soil moisture at local scale, and a high diversity of land cover types at the landscape scale affected ant species composition by promoting higher nest densities of some species. Ant community evenness was high in wetter grasslands with low within-site variability in soil moisture and surrounded by a less diverse landscape. Fourth-corner models revealed that ant community structure response to environmental heterogeneity was mediated mainly by worker size, colony size, and life history traits related with colony reproduction and foundation. We discuss how within-site local variability in soil moisture and low-intensity grazing promote ant species densities and highlight the role of habitat temperature and humidity affecting community evenness. We hypothesize that a higher diversity of land cover types in a forest-dominated landscape buffers less favorable environmental conditions for ant species establishment and dispersal between grasslands. We conclude that spatial environmental heterogeneity at local and landscape scale plays an important role as deterministic force in filtering ant species and, along with neutral processes (e.g., stochastic colonization), in shaping ant community structure in temperate seminatural upland grasslands.

Uncertainties in the adaptation of alpine pastures to climate change based on remote sensing products and modelling.

Over the last century, the management of pastoral systems has undergone major changes to meet the livelihood needs of alpine communities. Faced with the changes induced by recent global warming, the ecological status of many pastoral systems has seriously deteriorated in the western alpine region. We assessed changes in pasture dynamics by integrating information from remote-sensing products and two process-based models, i.e. the grassland-specific, biogeochemical growth model PaSim and the generic crop-growth model DayCent. Meteorological observations and satellite-derived Normalised Difference Vegetation Index (NDVI) trajectories of three pasture macro-types (high, medium and low productivity classes) in two study areas - Parc National des Écrins (PNE) in France and Parco Nazionale Gran Paradiso (PNGP) in Italy - were used as a basis for the model calibration work. The performance of the models was satisfactory in reproducing pasture production dynamics (R2 = 0.52 to 0.83). Projected changes in alpine pastures due to climate-change impacts and adaptation strategies indicate that: i) the length of the growing season is expected to increase between 15 and 40 days, resulting in changes in the timing and amount of biomass production, ii) summer water stress could limit pasture productivity; iii) earlier onset of grazing could enhance pasture productivity; iv) higher livestock densities could increase the rate of biomass regrowth, but major uncertainties in modelling processes need to be considered; and v) the carbon sequestration potential of pastures could decrease under limited water availability and warming.

Response of soil health indicators to dung, urine and mineral fertilizer application in temperate pastures.

Healthy soils are key to sustainability and food security. In temperate grasslands, not many studies have focused on soil health comparisons between contrasting pasture systems under different management strategies and treatment applications (e.g. manures and inorganic fertilisers). The aim of this study was to assess the responses of soil health indicators to dung, urine and inorganic N fertiliser in three temperate swards: permanent pasture not ploughed for at least 20 years (PP), high sugar ryegrass with white clover targeted at 30% coverage reseeded in 2013 (WC), and high sugar ryegrass reseeded in 2014 (HG). This study was conducted on the North Wyke Farm Platform (UK) from April 2017 to October 2017. Soil health indicators including soil organic carbon (SOC, measured by loss of ignition and elemental analyser), dissolved organic carbon (DOC), total nitrogen (TN), C:N ratio, soil C and N bulk isotopes, pH, bulk density (BD), aggregate stability, ergosterol concentration (as a proxy for fungi biomass), and earthworms (abundance, mass and density) were measured and analysed before and after application of dung and N fertilizer, urine and N fertiliser, and only N fertiliser. The highest SOC, TN, DOC, ergosterol concentration and earthworms as well as the lowest BD were found in PP, likely due to the lack of ploughing. Differences among treatments were observed due to the application of dung, resulting in an improvement in chemical indicators of soil health after 50 days of its application. Ergosterol concentration was significantly higher before treatment applications than at the end of the experiment. No changes were detected in BD and aggregate stability after treatment applications. We conclude that not enough time had passed for the soil to recover after the ploughing and reseeding of the permanent pasture, independently of the sward composition (HG or WC). Our results highlight the strong influence of the soil management legacy in temperate pasture and the positive effects of dung application on soil health over the short term. In addition, we point out the relevance of using standardised methods to report soil health indicators and some methodological limitations.

Species richness and adaptation of the invasive cattle tick, Rhipicephalus microplus (Acari: Ixodidae) on camps grazed by sheep in the Eastern Cape Province, South Africa.

The seasonal dynamics and distribution of ticks play a pivotal role in the epidemiology of tick-borne diseases. The objective of this study was to establish the seasonal abundance and species richness of questing ixodid ticks on Amathole Montane Grassland camps grazed by sheep. Ticks questing for hosts were collected monthly for a period of three years by dragging flannel strips attached to a wooden spar over the vegetation. At each occasion, six replicate drag-samples were made in camps grazed by sheep. Of the questing ticks (n = 14 891) collected from the vegetation, the most abundant larvae were those of Rhipicephalus microplus (95.04%) followed by Rhipicephalus appendiculatus (2.32%), Rhipicephalus evertsi (1.56%), Rhipicephalus decoloratus (1.03%), Rhipicephalus simus (0.03) and Amblyomma hebraeum (0.02%). Comparing the two Rhipicephalus (Boophilus) spp., R. microplus (98.9%) outcompete the indigenous tick, R. decoloratus (1.1%). The R. microplus larvae were significantly higher (P < 0.05) in 2015 (2.11±0.108), 2016 (2.02 ± 0.076) and 2017 (1.94±0.075) during spring than any other season. There were no significant differences (P ˃ 0.05) from R. appendiculatus questing ticks collected in autumn (0.27±0.007; 0.30±0.052) and spring (0.33±0.007; 0.20±0.052) for 2015 and 2016, respectively. The study showed that the cattle tick, R. microplus adapted very well on host species, in this case sheep, and encroached to areas that were too cold for its adaptation.

Bradyrhizobium occurrence in nodules of perennial horsegram.

The introduction of a forage legume into a tropical pasture should decrease the need for N fertilizer, provided biological N2 fixation (BNF) contributes enough to compensate for exported N. Macrotyloma axillare (perennial horsegram) is a suitable legume for composing mixed pastures, and our hypothesis is that the isolation of indigenous rhizobia from roots and rhizosphere is the way of achieving an efficient inoculant to maximize BNF to the legume. Nodules and rhizosphere soil taken from M. axillare grown in a mixed pasture with palisade grass were sampled and used in a trap host assay using Leonard jars containing a mixture of vermiculite and sand. A total of ten bacteria were initially isolated using this technique. The isolates were then used in two experiments to evaluate the inoculation responses on the perennial horsegram in greenhouse conditions to which nodulation, plant growth, and shoot N accumulation were measured. Phylogenetic analyses based on 16S rRNA and recA placed all strains within genus Bradyrhizobium, some of them not previously described. The best strain provided more than 120 nodules and more than 65 mg of nodules per plant. Strain BR14182 was considered the most promising given the high dry matter and N accumulation in plant shoots. This study provides the first analysis of Bradyrhizobium diversity nodulating M. axillare in Brazil and provided evidence of the role of inoculation in incrementing the plant-rhizobium symbiosis in a forage legume.

Seasonal Variations in Voluntary Intake and ApparentDigestibility of Forages by Goats in the Chinese Altai Mountains.

Forage availability and quality directly impact animal performance, ultimately affecting productivity and health. This study aimed to understand the interaction between qualitative and quantitative vegetation availability and feed intake of goats on alpine pastures in the Chinese Altai Mountains. The daily grazing routes of three goats from a local herding family were monitored with GPS devices set at a logging rate of 64 s during spring and the early and late summer season in 2013 and 2014. The quantity and quality of vegetation along their grazing routes was determined, and the amount of feces excreted was measured in a total of five goats per season for the indirect determination of the animals' feed intake. The grazing routes were longer in spring than in summer, leading to larger grazing areas visited in spring. Vegetation on offer ranged from 980 to 2400 kg dry mass per hectare and was similar in the spring and summer seasons but higher in 2013 than in 2014. Feed consumption of forage and nutrients did not significantly differ between seasons and years, respectively, suggesting that the goats' nutrient intake was not restricted by interannual variability of forage on offer. Regular monitoring of animal numbers and of vegetation quantity and quality on the mountain rangelands can help responsible government agencies to estimate forage offtake of small ruminants in order to timely adjust grazing pressure in the study region.