Health-Related Composition and Bioactivity of an Agave Sap/Prickly Pear Juice Beverage.

In this study, a beverage made from a combination of Agave sap (AS) and prickly pear juice (PPJ) was analyzed for its nutrients and bioactive and potentially health-promoting compounds. The beverage was evaluated for its ability to act as an antioxidant, regulate glycemic properties, and undergo gut bacterial fermentation in vitro. The major mono- and oligosaccharides present in the beverage were galacturonic acid (217.74 ± 13.46 mg/100 mL), rhamnose (227.00 ± 1.58 mg/100 mL), and fructose (158.16 ± 8.86 mg/mL). The main phenolic compounds identified were protocatechuic acid (440.31 ± 3.06 mg/100 mL) and catechin (359.72 ± 7.56 mg/100 mL). It was observed that the beverage had a low glycemic index (<40) and could inhibit digestive carbohydrases. The combination of ingredients also helped to reduce gas production during AS fermentation from 56.77 cm3 to 15.67 cm3. The major SCFAs produced during fermentation were butyrate, acetate, and propionate, with valerate being produced only during the late fermentation of the AS. This beverage is rich in bioactive compounds, such as polyphenols and dietary fiber, which will bring health benefits when consumed.

Interactions of Opuntia ficus-indica with Dactylopius coccus and D. opuntiae (Hemiptera: Dactylopiidae) through the Study of Their Volatile Compounds.

Opuntia ficus-indica has always interacted with many phytophagous insects; two of them are Dactylopius coccus and D. opuntiae. Fine cochineal (D. coccus) is produced to extract carminic acid, and D. opuntiae, or wild cochineal, is an invasive pest of O. ficus-indica in more than 20 countries around the world. Despite the economic and environmental relevance of this cactus, D. opuntiae, and D. coccus, there are few studies that have explored volatile organic compounds (VOCs) derived from the plant-insect interaction. The aim of this work was to determine the VOCs produced by D. coccus and D. opuntiae and to identify different VOCs in cladodes infested by each Dactylopius species. The VOCs (essential oils) were obtained by hydrodistillation and identified by GC-MS. A total of 66 VOCs from both Dactylopius species were identified, and 125 from the Esmeralda and Rojo Pelón cultivars infested by D. coccus and D. opuntiae, respectively, were determined. Differential VOC production due to infestation by each Dactylopius species was also found. Some changes in methyl salicylate, terpenes such as linalool, or the alcohol p-vinylguaiacol were related to Dactylopius feeding on the cladodes of their respective cultivars. Changes in these VOCs and their probable role in plant defense mechanisms should receive more attention because this knowledge could improve D. coccus rearing or its inclusion in breeding programs for D. opuntiae control in regions where it is a key pest of O. ficus-indica.

Climatic and soil characteristics account for the genetic structure of the invasive cactus moth Cactoblastis cactorum, in its native range in Argentina.

Background: Knowledge of the physical and environmental conditions that may limit the migration of invasive species is crucial to assess the potential for expansion outside their native ranges. The cactus moth, Cactoblastis cactorum, is native to South America (Argentina, Paraguay, Uruguay and Brazil) and has been introduced and invaded the Caribbean and southern United States, among other regions. In North America there is an ongoing process of range expansion threatening cacti biodiversity of the genus Opuntia and the commercial profits of domesticated Opuntia ficus-indica. Methods: To further understand what influences the distribution and genetic structure of this otherwise important threat to native and managed ecosystems, in the present study we combined ecological niche modeling and population genetic analyses to identify potential environmental barriers in the native region of Argentina. Samples were collected on the host with the wider distribution range, O. ficus-indica. Results: Significant genetic structure was detected using 10 nuclear microsatellites and 24 sampling sites. At least six genetic groups delimited by mountain ranges, salt flats and wetlands were mainly located to the west of the Dry Chaco ecoregion. Niche modeling supports that this region has high environmental suitability where the upper soil temperature and humidity, soil carbon content and precipitation were the main environmental factors that explain the presence of the moth. Environmental filters such as the upper soil layer may be critical for pupal survival and consequently for the establishment of populations in new habitats, whereas the presence of available hosts is a necessary conditions for insect survival, upper soil and climatic characteristics will determine the opportunities for a successful establishment.

Application of soil herbicides in gardening to improve fruit production / Aplicação de herbicidas de solo na jardinagem para melhorar a produção de frutas

The study considered the use of soil herbicides: Begin Turbo, KS; Dual Gold, KE; Euro-Lighting, VRK; Command, KE; Pivot, VK; Proponite, KE; Zenkor Ultra, KS and partially soil action: Demetra, KE, and Dialen Super, KS. We conducted a comparative assessment of the biological effectiveness of the studied herbicides against the main species of weeds present in the experimental plots, annual and perennial dicotyledonous, annual cereal weeds. The effect of soil herbicide treatments on the physiological state of plants of apple, pear, walnut, and black currant was studied. The effect of the use of the studied drugs on the yield of protected crops for three years was evaluated. The tests proved the applicability of soil herbicides in nursery, production gardens, as well as on seedlings with a closed root system. The tested products, despite the principle of their action - penetration into weeds through the soil, did not harm the protected crops, no negative effect on the growth of trees and shrubs was recorded. The study revealed no evidence that drugs had a negative impact on fruit and berry crop productivity. There are suggestions for improving the efficacy of using soil herbicides when planting fruit and berry crops.

O estudo considerou o uso dos herbicidas de solo: Begin Turbo, KS; Dual Gold, KE; Euro-Lighting, VRK; Command, KE; Pivot, VK; Proponite, KE; Zenkor Ultra, KS e ação parcial do solo: Demetra, KE e Dialen Super, KS. Realizou-se uma avaliação comparativa da eficácia biológica dos herbicidas estudados contra as principais espécies de plantas daninhas presentes nas parcelas experimentais, dicotiledôneas anuais e perenes, plantas daninhas de cereais anuais. Estudou-se o efeito de tratamentos com herbicidas de solo sobre o estado fisiológico de plantas de macieira, pera, nogueira e groselha preta. Foi avaliado o efeito do uso das drogas estudadas na produtividade de cultivos protegidos por três anos. Os testes comprovaram a aplicabilidade de herbicidas de solo em viveiros, hortas de produção, bem como em mudas com sistema radicular fechado. Os produtos testados, apesar de seus princípios de ação - penetração em ervas daninhas através do solo, não prejudicou as culturas protegidas, não foi registrado nenhum efeito negativo no crescimento de árvores e arbustos. O estudo não revelou evidências de que as drogas tenham um impacto negativo na produtividade das frutas e bagas. Existem sugestões para melhorar a eficácia do uso de herbicidas de solo ao plantar frutas e bagas.

Antioxidant Edible Films Based on Pear Juice and Pregelatinized Cassava Starch: Effect of the Carbohydrate Profile at Different Degrees of Pear Ripeness.

Edible films based on fruit and vegetable purees combined with different food-grade biopolymeric binding agents (e.g., pectin, gelatin, starch, sodium alginate) are recognized as interesting packaging materials that benefit from the physical, mechanical, and barrier properties of biopolymers as well as the sensory and nutritional properties of purees. In the current contribution, edible antioxidant films based on pear juice and pregelatinized cassava starch were developed. In particular, the suitability of using pregelatinized cassava starch for the non-thermal production of these novel edible films was evaluated. In addition, the effects on the films' properties derived from the use of pear juice instead of the complete puree, from the content of juice used, and from the carbohydrate composition associated with the ripening of pears were all studied. The produced films were characterized in terms of their total polyphenol content, water sensitivity, and water barrier, optical, mechanical and antioxidant properties. Results showed that the use of pear juice leads to films with enhanced transparency compared with puree-based films, and that juice concentration and carbohydrate composition associated with the degree of fruit ripeness strongly govern the films' properties. Furthermore, the addition of pregelatinized cassava starch at room temperature discloses a significant and favorable impact on the cohesiveness, lightness, water resistance, and adhesiveness of the pear-juice-based films, which is mainly attributed to the effective interactions established between the starch macromolecules and the juice components.

Fermentation Patterns, Methane Production and Microbial Population under In Vitro Conditions from Two Unconventional Feed Resources Incorporated in Ruminant Diets.

In this study, four experimental treatments were evaluated: (T1) alfalfa hay + concentrate, (50:50%, DM); (T2) alfalfa hay + Leucaena leucocephala + concentrate, (30:20:50%, DM); (T3) alfalfa hay + prickly pear + concentrate, (30:20:50%, DM); and (T4) alfalfa hay + Leucaena leucocephala + prickly pear + concentrate, (30:10:10:50%, DM). NH3-N concentrations in T2 and T4 decreased when replaced with alfalfa hay in 20 and 10%, respectively. Treatments did not affect the concentration of total volatile fatty acids (TVFA) between T3 and T4 (p > 0.05), while the concentrations among T1 and T2 were different (p < 0.05). T2 showed a reduction of 25.5% in the methane production when compared to T1 (p < 0.05). The lowest concentrations of protozoa were observed in T2 and T4, which contained Leucaena leucocephala (T2) and Leucaena leucocephala + prickly pear (T4) (p < 0.05). The highest concentration of total methanogens was recorded in T1 and was different in T2, T3, and T4 (p < 0.05). Leucaena leucocephala, at an inclusion percentage of 20%, decreased the methane when compared to T1, whereas prickly pear increased methane production in relation to T1.

Assessment of Prickly Pear Fruit Peel Mucilage in Form of Gel as a Green Coagulant for the Tertiary Treatment of Domestic Wastewater.

The application of natural coagulants derived from food byproducts in domestic wastewater tertiary treatment, which contains a number of impurities as suspended colloidal particles, has a potential use as essential substitutes for traditional inorganic coagulants. These biomaterials are a sustainable and environmentally friendly alternative that can be used to improve water quality and human health. In this study, prickly pear (PP) fruit peel mucilage gel was evaluated as a novel coagulant for the tertiary stage of domestic wastewater treatment. Jar tests were performed on residual raw water at the inlet (influent) and outlet (effluent) of the tertiary wastewater treatment (constructed wetland) with a coagulant dose of 12 mg L-1 at a pH of 13. The efficiency of green (i.e., mucilage) and inorganic chemical (i.e., FeCl3) coagulants was compared on the basis of turbidity and color removal. The flocs produced by the coagulants were characterized structurally by FTIR spectroscopy and Zeta potential analysis and morphologically by scanning electron microscopy (SEM). The results showed that the turbidity and the color removal efficiency of the mucilage compared to the FeCl3 at the outlet of the treatment (effluent) were practically the same, reaching 94% turbidity and 85-87% color removal efficiency with both coagulants. The structure and morphology of the flocs generated by the coagulants showed a higher content of organic matter trapped in the flocs. The floc formation observed mechanisms were adsorption/bridging for mucilage and charge neutralization for FeCl3. The results of this study demonstrated that the PP mucilage green coagulant can be used to enhance the quality of treatment of domestic wastewater in an eco-friendly and biodegradable manner.

Bioactive Compounds and Sensory Analysis of Freeze-Dried Prickly Pear Fruits from an Inter-Andean Valley in Peru.

Prickly pear fruits are seasonal and have shades ranging from pale green to deep purple. Their pigments are associated with bioactive compounds, being sensitive to thermal transformation processes for their conservation. The objective of this research was to evaluate the bioactive compounds and the sensory analysis of freeze-dried prickly pear fruits from an inter-Andean valley in Peru. The prickly pear fruits of the morada, anaranjada, and blanca ecotypes came from an inter-Andean valley in Peru at 2972 m altitude. The sliced fruits were freeze-dried at -40 °C and 0.25 mTorr, and the total polyphenol content (TPC), vitamin C, and antioxidant activity (AA) were determined, as well as the color L* a* b*, color index (CI*), FTIR spectra, and mineral content. In the same way, sensory analysis of preferences with nine scales was applied. It was observed that in the freeze-dried fruits, TPC, AA, and vitamin C increased significantly (p-value < 0.05), and their corresponding functional groups increased in intensity in their corresponding FTIR spectra; furthermore, trace elements such as Cu, Fe, Se, Zn, Si, and Mn were identified. On the other hand, freeze-drying provided deeper colors to the fruits, which most panelists said they "very much liked" during the sensory analysis, although the texture was not very well accepted, with most panelists reporting being "indifferent" towards it. The freeze-drying technique allows the bioactive and sensory attributes of prickly pear fruits from inter-Andean valleys to be preserved, making it a potential fruit for export and conservation due to its seasonality.

Extract from Opuntia ficus-indica cladode delays the Aedes aegypti larval development by inducing an axenic midgut environment.

This study evaluated the effects of acute exposure of Aedes aegypti third instar (L3 ) larvae to the saline extract of Opuntia ficus-indica cladodes on the biological cycle and fertility of the emerging adults. For this, larvae were treated for 24 h with the extract at » LC50 (lethal concentration to kill 50% of larvae), ½ LC50 or LC50 ; the development and reproduction of the emerged adults were evaluated after a recovery period of 9 days. The resistance of proteins in the extract to hydrolysis by L3 digestive enzymes and histomorphological alterations in the larval midgut were also investigated. The extract contained lectin, flavonoids, cinnamic derivatives, terpenes, steroids, and reducing sugars. It showed a LC50 of 3.71% for 48 h. The data indicated mean survival times similar in control and extract treatments. It was observed development delay in extract-treated groups, with a lower number of adults than in control. However, the females that emerged laid similar number of eggs in control and treatments. Histological evaluation revealed absence of bacterial and fungal microorganisms in the food content in midguts from larvae treated with cladode extract. Electrophoresis revealed that three polypeptides in the extract resisted to hydrolysis by L3 digestive proteases for 90 min. The lectin activity was not altered even after 24-h incubation with the enzymes. In conclusion, the extract from O. ficus-indica can delay the development of Ae. aegypti larvae, which may be linked to induction of an axenic environment at larval midgut and permanence of lectin activity even after proteolysis.

Effect of replacing corn with cactus pear on the performance and carcass traits and meat quality of feedlot finished lambs / Efeito da substituição do milho pela palma forrageira sobre o desempenho, características de carcaça e qualidade da carne de cordeiros terminados em confinamento

The aim was to evaluate the effect of replacing ground corn with spineless cactus from two species Orelha de Elefante Mexicana-OEM (Opuntia stricta Haw.) and Gigante (Opuntia ficus-indica Mill) in a total mixed ration for finishing lambs evaluating its effects on intake, ingestive behavior, performance, carcass traits, commercial cuts and physiochemical composition of the meat. Eighteen crossbred lambs with average body weight of 15.0 ± 2.32 kg were distributed in a completely randomized design with three treatments (OEM and Gigante spineless cactus and ground corn as control treatment) and six replications. Spineless cactus species replacing ground corn in lambs diets does not change the intake of dry matter (DMI), crude protein and neutral detergent fiber or time (min/d) of ingestion, rumination and idleness, or final body weight gain. Lambs fed with ground corn and "Gigante" cactus presented a higher total weigh gain (TWG) and average daily weight gain (ADG) when compared to OEM spineless specie. Ground corn diet promoted better hot and cold carcass yield in lambs receiving spineless cactus, regardless of species. Feed and total costs (kg/lamb) were higher for the ground corn diet. The replacement of ground corn with spineless cactus did not change moisture, protein, and ash meat contents, as well as water holding capacity, cooking loss, shear force, and a* color intensity. However, there was an effect for the meat lipid content, b* and L* color intensity and color index, where the species of cactus Gigante on ground corn presented the highest lipid content and yellowness (b*) intensity and lower L* color compared to OEM. Spineless cactus species Gigante can replace ground corn as a source of energy in diets for finishing lambs because it significantly improves the financial income for the producer without changing the ADG, DMI, ingestive behavior and yield of commercial cuts.

Objetivou-se avaliar o efeito da substituição do milho moído por duas espécies de palma forrageira, Orelha de Elefante Mexicana-OEM (Opuntia stricta Haw.) e espécie Gigante (Opuntia ficus-indica Mill) em uma ração completa para terminação de cordeiros avaliando seus efeitos sobre consumo, comportamento ingestivo, desempenho, características de carcaça, cortes comerciais e composição físico-química da carne. Foram utilizados dezoito cordeiros sem padrão racial definido com peso corporal médio de 15,0 ± 2,32 kg e foram distribuídos em delineamento inteiramente casualizado com três tratamentos (OEM e palma Gigante e grão de milho como controle) e seis repetições. As duas espécies de palma forrageira em substituição ao milho moído na dieta de cordeiros não modificaram o consumo de matéria seca (CMS) proteína bruta e fibra em detergente neutro nem o tempo (min/dia) de ingestão, ruminação e ócio ou ganho corporal final. Cordeiros alimentados com milho moído e palma Gigante apresentaram maior ganho de peso total (GPT) e médio diário (GMD) em relação à espécie OEM. A dieta com milho moído promoveu melhor rendimento de carcaça quente e fria comparando cordeiros recebendo palma forrageira, independente da espécie. Os custos com ração e total (kg/cordeiro) foram maiores para a dieta com milho moído. A substituição do milho moído pela palma forrageira não alterou os teores de umidade, proteína e cinzas da carne, bem como a capacidade de retenção de água, perda por cozimento, força de cisalhamento e intensidade de cor a*. No entanto, houve efeito para o teor de lipídios da carne, índice de intensidade de cor b* e L*, sendo que a espécie de palma Gigante e o milho grão moído apresentou o maior teor de lipídios e intensidade de amarelo (b*) e menor cor L* em relação ao OEM. A espécie palma forrageira Gigante pode substituir o milho moído como fonte de energia em dietas para cordeiros em terminação, pois melhora significativamente o rendimento financeiro do produtor sem alterar o GMD, CMS, comportamento ingestivo e rendimento dos cortes comerciais.

Genetic diversity and relationships among Nopalea sp. and Opuntia spp. accessions revealed by RAPD, ISSR and ITS molecular markers.

BACKGROUND: Dactylopius opuntiae (Cockerell) (carmine cochineal) is an insect pest highly noxious that has spread through cactus pear crops in the Brazilian semiarid region. Knowledge of diversity and genetic relationships of the cactus pear accessions is fundamental to create new varieties resistance to carmine cochineal. Therefore, this investigation was undertaken to assess the genetic diversity and genetic relationships that existed among cactus pear accessions of Nopalea sp. and Opuntia spp. with contrasting resistance to D. opuntiae. METHODS AND RESULTS: We conducted a molecular analysis in seven cactus pear accessions from the "reference collection" of the Agronomic Institute of Pernambuco, Brazil using RAPD, ISSR and ITS molecular markers. A total of 242 bands were detected from 26 polymorphic primers. The high percentage of polymorphism by RAPD (89.8%), ISSR (81.2%) and ITS (75%) markers suggests that the cactus pear accessions have high genetic diversity. The combined analysis of markers systems enabled the accessions discrimination of about the genus and ploidy, but were incongruous in relation to resistance level to D. opuntiae. CONCLUSIONS: Genetic diversity, discrimination of about the genus and ploidy was confirmed by merging information from ISSR, RAPD and ITS markers systems. The IPA-200016, IPA-200149, IPA-100004, IPA-200205 accessions are genetically divergent, therefore could be potentially incorporated into any further breeding programs directed to create new varieties of cactus pear resistant to D. opuntiae.

Physical and Dietary Intervention with Opuntia ficus-indica (Nopal) in Women with Obesity Improves Health Condition through Gut Microbiota Adjustment.

Obesity is a multifactorial disease resulting in excessive accumulation of fat. Worldwide, obesity is an important public health problem, affecting a large proportion of the world population. The tender cactus Opuntia ficus-indica, commonly known in Mexico as "nopal", is widely distributed in this country, Latin America, South Africa, and the Mediterranean area. Nopal cladodes are commonly marketed in different forms as fresh, frozen, or pre-cooked, and used as fresh green vegetable. The aim of this study was to evaluate the capability of nopal to improve the health condition of participants affected by obesity, in a physical and dietary intervention, through gut microbiota modification. These results were contrasted with the effect of nopal in the gut microbiota of normal weight participants. We describe the association among biochemical, anthropometric markers, and the gut microbiota diversity found in fecal samples of the obese and normal weight groups. The results presented in this work suggest that caloric restriction, addition of nopal to the diet and physical activity, promote changes in the gut microbiota in obese women, improving the host metabolism, as suggested by the correlation between some bacterial species with biochemical and anthropometrical parameters.

Identification and characterization of Colletotrichum species associated with anthracnose on persimmon in Brazil.

Persimmon (Diospyros kaki) anthracnose is a major threat in production areas worldwide. Most of the studies are focused on Colletotrichum horii, but other species have been reported as well. The association of distinct Colletotrichum species present in Brazilian persimmon production regions as well as their host ranges are yet elusive. The aims of this work were to identify and characterize Colletotrichum species associated with the persimmon anthracnose. In a survey performed in four production regions of Brazil, 88.7% and 11.3% out of 231 isolates were identified as members of Colletotrichum gloeosporioides species complex (Cgc) or Colletotrichum acutatum species complex (Cac), respectively. A subset of 18 isolates were identified through multilocus phylogenetic analysis, using ITS-rDNA region and two loci, namely GAPDH and TUB2. This study revealed the presence of four species: C. horii (38.8%) and Colletotrichum fructicola (27.7%) from the Cgc and Colletotrichum nymphaeae (27.7%) and Colletotrichum melonis (5.8%), from the Cac. Additionally, 13 isolates were selected for morphological, physiological, and pathogenic analyses. Contrasting characteristics were observed among species of the Cgc and Cac complexes. The optimal temperature for mycelial growth and germination were higher for Cgc species. The percentage of appressoria melanisation also varied across complexes. All the identified species were able to cause anthracnose-like symptoms on persimmon fruit, leaves, shoots, and sepals. Colletotrichum species from persimmon were also able to infect apple and pear. The findings will support decisions to manage anthracnose of persimmon under high infection risk due to multiple host susceptibility.

Physicochemical Composition and Fatty Acid Profile of Goat Kids' Meat Fed Ground-Corn-Grain Silage Rehydrated with Different Additives.

The effects of the replacement of dry ground corn (GC) with corn-grain silage rehydrated with water (RCSwater), cactus pear mucilage (RCSmucilage), and whey (RCSwhey) on the growth, physicochemical composition, and fatty acid profile of goat kids' meat were investigated. Thirty-two crossbred goat kids (16.4 ± 2.50 kg initial weight) were assigned in a randomized block design with four treatments and eight repetitions. The NDF intake of goat kids fed with RCSmucilage was higher in comparison to RCSwater and RCSwhey (p = 0.0009). The dietary replacement of GC by RCSmucilage increased the final weight (p = 0.033) and meat-cooking losses (p = 0.0001) of kids. The concentrations of oleic (p = 0.046), 11,14-eicosadienoic (p = 0.033), and EPA (p = 0.010) were higher in the meat of kids fed with RCSmucilage and RCSwhey, and the α-linolenic concentration was higher (p = 0.019) for animals feeding with RCSmucilage. Meat from kids fed with RCSwhey presented the lowest ∑SFA and the highest ∑MUFA. In contrast, the ∑PUFA (p < 0.012) was higher for goats fed with RCSwater. The ∑ω3 (p < 0.0001) was higher in animals fed with RCSmucilage and RCSwhey. Desirable fatty acids were higher (p = 0.044) in animals fed with RCSmucilage and RCSwhey, and the atherogenicity (p = 0.044) and thrombogenicity (p < 0.0001) indexes were lower for goats fed the RCSwhey diet. The enzymatic activities of Δ9desaturase (C16) were higher (p = 0.027) in goat kids fed with GC and RCSmucilage, and Δ9desaturase (C18) was higher (p = 0.0497) when goats were fed with RCSmucilage and RCSwhey. Elongase activities were higher (p = 0.045) in goat kids fed with GC and RCSwater. The total replacement of GC by RCSmucilage is recommended in the diet of goat kids due to improvements in the weight gain and proportion of desirable fatty acids in the meat. In addition, RCSmucilage promoted better conservation of the silage at a lower cost when compared to commercial additives.

Effect of regulated deficit irrigation (RDI) on the vegetative growth of pear variety Triunfo de Viena (Pyrus communis L.) / Efecto del riego deficitario controlado (RDC) en el crecimiento vegetativo del peral variedad Triunfo de Viena (Pyrus communis L.)

ABSTRACT One of the consequences of a water deficit in the vegetative growth is the modification of the plant vigor, which determines the extent and the rate of growth and lengthening in shoots, which, in this phase, is more important than fruit growth, which is very slow in terms of dry mass accumulation. The objective of this research was to determine the effect on the vegetative growth of the pear variety Triunfo de Viena (Pyrus communis L) of regulated deficit irrigation (RDI), which was compared with a control irrigated at 100% crop evapotranspiration (ETc). The irrigation treatments consisted of the application of watering regimes of 74 and 48% ETc in treatment T2 and 60 and 27% ETc in treatment T3 in 2014 and 2015, respectively, during the period of rapid fruit growth with the same watering regime used in T1 (control) applied during the rest of the season. The irrigation reduction used in T2 and T3 represented water savings of 26% and 40% in 2014 and 52% and 73% in 2015, respectively. In the deficit treatments, there were no significant differences with respect to the control for the length, or absolute and relative growth rates (AGR and RGR) of the shoots. The weight of the wood from the fructification pruning had a significant difference between T3 and the control.

RESUMEN Una de las consecuencias del déficit hídrico en el crecimiento vegetativo es la modificación del vigor de la planta, que determina la extensión y el ritmo de crecimiento y de alargamiento de los brotes que, en esta fase, es más importante que el crecimiento del fruto, el cual, es bastante lento, en términos de acumulación de masa seca. El objetivo de esta investigación fue determinar el efecto sobre el crecimiento vegetativo del peral variedad Triunfo de Viena (Pyrus communis L.) del riego deficitario controlado (RDC), que se comparó con un Control, regado al 100%, de la evapotranspiración del cultivo ETc. Los tratamientos de riego consistieron en la aplicación de láminas de agua, correspondiente al 74 y 48% de la ETc, en el tratamiento T2 y 60 y 27% de la ETc, en el tratamiento T3, en 2014 y 2015, respectivamente; durante el periodo de crecimiento rápido del fruto, mientras el resto de la temporada, todos se regaron igual que el T1 (control). La reducción de la lámina de riego en T2 y T3, durante este periodo, representó un ahorro de agua de 26 y 40%, en 2014 y 52 y 73%, en 2015, respectivamente. En los tratamientos deficitarios no hubo diferencias significativas respecto al control, en el diámetro, la longitud, la tasa absoluta y la relativa de crecimiento (TAC y TRC) de brotes. En la masa de madera correspondiente a la poda de fructificación, se presentó diferencia significativa, entre el T3 y el control.

Evaluation of cryotolerant yeasts for the elaboration of a fermented pear beverage in Patagonia: Physicochemical and sensory attributes.

The production of pome fruits as pears and apples, as well as their derived industries, is of great economic importance in North Patagonia. The elaboration of fermented beverages as cider or perry has evidenced a substantial diversification during the last years, with the evaluation of different fruit varieties, yeast starters and technological changes. In this work, two cryotolerant yeasts belonging to the species Saccharomyces uvarum were evaluated at laboratory and pilot scale in sterile and no-sterile pear must. One of the strains was originally isolated from apple chicha (strain NPCC1314) and the other from apple cider (strain NPCC1420) in Patagonia. Both physicochemical and sensory features of the fermented products were evaluated. Both strains were able to successfully complete the fermentations, although strain NPCC1420 showed the better kinetic properties including a faster sugar consumption than the strain NPCC1314. Both strains showed excellent implantation capacity, but the fermented products showed different chemical profiles. The perry fermented with the strain NPCC1314 was characterized by better sensory attributes as assessed by trained panelists and a greater acceptance for untrained public than the same fermented with the strain NPCC1420. The two strains were able to consume sorbitol, both in pear must and in agar-plates supplemented with sorbitol as the sole carbon source. This ability is described for the first time in S. uvarum, at least for the two strains evaluated in this work.

Sensory attributes, physicochemical and antioxidant characteristics, and protein profile of wild prickly pear fruits (O. macrocentra Engelm., O. phaeacantha Engelm., and O. engelmannii Salm-Dyck ex Engelmann.) and commercial prickly pear fruits (O. ficus-indica (L.) Mill.).

Mexico presents the highest richness of Opuntia Mill. species. These species are an important economic factor for the country, and source of nutrients, bioactive compounds, pigments, and nutraceuticals which can be of interest for the food and pharmaceutical industry. However, there are some wild Opuntia species in the Chihuahua desert, that have not been analyzed to establish their properties and potential use. The aim of study was to evaluate the sensory, physicochemical and protein profile in wild prickly pear fruits (O. macrocentra Engelm. (OM), O. phaeacantha Engelm. (OP), and O. engelmannii Salm-Dyck ex Engelmann. (OE)) from Samalayuca, Chihuahua and compare them with two commercial prickly pear fruits (O. ficus-indica (L.) Mill. (green-OFG, red-OFR). The sensory profile of wild species was characterized by highest color, odor, and sour taste compared to the commercial fruits. Pulp, peel, and seeds from wild prickly pear fruits showed lower pH, and higher titratable total acidity, total phenolic compounds, total flavonoids, antioxidant capacity, protein, lipids, ash, carbohydrates (only peel), and crude fiber content than commercial Opuntia species. Furthermore, O. engelmannii showed a tendency to present the highest betacyanins, betaxanthins, and betalains contents. A total of 181, 122, 113, 183 and 140 different proteins were identified in OM, OP, OE, OFG, OFR species, respectively. All species showed the highest enrichment in three main pathways such as amino acids biosynthesis, glycolysis (dark)/gluconeogenesis (light), and the citric acid cycle. The wild prickly pear fruits of this study showed important nutritional, protein, and antioxidant properties with biological interest, and can be a potential source of functional ingredients and nutraceuticals.

Stabilisation of betalains and phenolic compounds extracted from red cactus pear (Opuntia ficus-indica) by spray and freeze-drying using oca (Oxalis tuberosa) starch as drying aid.

The objective of this work was to evaluate the stabilisation of betalains and phenolic compounds extracted from red cactus pear by spray and freeze-drying. After hydroethanolic extraction and partial solvent removal under reduced pressure, the highly coloured extracts were enriched with oca starch and maltodextrin as drying aids in different ratios (100:0, 70:30, and 50:50, oca starch: maltodextrin) prior to spray and freeze-drying. The obtained microencapsulated extract powders were characterised by moisture content, hygroscopicity, solubility and morphology. In addition, the stability of the encapsulated betalains and phenolics was evaluated during storage at room temperature for 105 days. All microcapsules showed high retentions of betacyanins (69.9-86.5% after 105 days), betaxanthins (72.2-81.9%), phenolic compounds (46.5-63.5%) and antioxidant capacity (60.1-64.9%, FRAP method; 49.7-57.5%, ABTS method). The system with 70:30 starch:maltodextrin ratio as drying aids showed the highest values of retention regarding the polyphenol content (63.5%), antioxidant capacity (64.9% to FRAP method) and betacyanin content (86.5%), as well as a low degradation rate constant of betacyanins (1.23 × 10-3 days-1) and a long half-life (563 days). Oca starch used alone or in combination with maltodextrin has been shown to work adequately as a microencapsulating agent and stabilizer of pigments and antioxidants derived from red cactus pear.

Nopal fiber (Opuntia ficus-indica) improves symptoms in irritable bowel syndrome in the short term: a randomized controlled trial.

BACKGROUND: Clinical guidelines provide limited and conflicting recommendations regarding dietary fiber supplementation in irritable bowel syndrome (IBS). Nopal (Opuntia ficus-indica) is a cactus plant fiber containing both insoluble and soluble fibers that may have therapeutic potential in IBS. Our aim was to evaluate the dose-response effect of extracted nopal fiber powder on symptoms in IBS. METHODS: We performed a 4-arm, double-blind, parallel, randomized controlled trial in 60 patients fulfilling Rome IV criteria for IBS. Patients were randomized and blindly allocated to receive either nopal fiber (10, 20, or 30 g/day) or placebo (30 g/day dextrose) for one week and to keep their usual diet. Symptom severity (Global Symptom Question, IBS-SSS, Gastrointestinal Symptom Rating Scale), stool frequency and consistency (Bristol Stool Form Scale), breath hydrogen response, and stool short-chain fatty acids (SCFA) were measured at baseline and follow-up. KEY RESULTS: Significantly more patients reported adequate relief of symptoms after 20 g/day (87%, p = 0.008) and 30 g/day (80%, p = 0.025) of nopal fiber compared to placebo (33%). More patients receiving 20 g/day nopal fiber (67%) had a > 50% reduction in IBS-SSS compared to placebo (20%, p = 0.027), whereas the 30 g/day dose induced more loose stools (p = 0.027). Response rates were similar among IBS subtypes. There were no differences in breath hydrogen or stool SCFA between groups. CONCLUSIONS AND INFERENCES: Nopal fiber supplementation at doses of 20 and 30 g/day was associated with short-term improvement in IBS symptoms, warranting a fully powered clinical trial of longer duration with symptomatic, physiological, and microbiological endpoints.

Cladosporium species causing "Cladosporium rot" on "Bosc" pear fruit in Argentina.

"Cladosporium rot" on "Bosc" pear fruit during cold storage causes significant economic losses and has been reported in recent years in the productive valleys of Río Negro and Neuquén. The species involved were not determined. During 2016-2017, "Bosc" pears (Pyrus communis) in cold storage chambers exhibited external brownish black circular spots caused by Cladosporium spp. The objective of this work was to determine the Cladosporium species that caused the above mentioned symptoms. The morphological and molecular analyses of the partial sequence of the actin gene (ACT) supported the identification. Cladosporium macrocarpum, Cladosporium subtilissimum and Cladosporium floccosum were determined as the species involved in the disease. Although Cladosporium has been reported to cause pear rot, this is the first report to identify these species as causal agents of this fruit disease.