Content Determination of Berberine Hydrochloride in Pessums by TLC-scanning / 中国药房

OBJECTIVE:To establish determine the content of berberine hydrochloride in the pessums.METHODS:Ben-zene-ethyl acetate-isopropanol-methanol-water(6∶3∶1.5∶1.5∶3)were taken as developers,which were detected under the UV light(365nm)and scanned by TLC scanner with the detective wavelength at430nm.RESULTS:The linear range of berberine hydrochloride on calibration curve was sample size0.25?g～1.25?g(r=0.9987),the average recovery rate was97.63%(RSD=1.88%).CONCLUSIONS:The method is simple,accurate and available for the quality control of pessums.