Enantioseparation and determination of alminoprofen in rat plasma and its application to a stereoselective pharmacokinetic study.

An enantioselective study of alminoprofen (AMF) in rat plasma was performed by a rapid, specific and sensitive chiral liquid chromatography-tandem mass spectrometry (LCMS/MS) method. A homemade ß-cyclodextrin (ß-CD) derivatized based chiral column and a commercial polysaccharide type chiral column were selected and evaluated. The former one per-4-chlorophenylcarbamate-ß-CD bonded chiral stationary phase (CSP) which was synthesized in our lab produced the enhanced enantioselective separation. The dextro-ketoprofen (d-KTF) was selected as the internal standard (IS). The approach was linear in the concentration ranges of 1.00-20000.0 ng mL-1 (r2≥0.99) for each enantiomer. The mean recoveries of R-(-)- and S-(+)-AMF at three spiked levels of 2.00, 1000.0 and 16000.0 ng mL-1 ranged from 92.0 %-96.1 %, and the intra-day and inter-day relative standard deviations (RSDs) were within 8.9 %. The lower limit of quantification (LLOQ) values of R-(-)- and S-(+)-AMF in rat plasma matrices were both 1.00 ng mL-1. The established method was successfully applied to a stereoselective pharmacokinetic study of AMF enantiomers in rat plasma following oral administration. The pharmacokinetic results revealed that S-(+)-AMF displayed prominently higher Cmax and AUC values than R-(-)-enantiomer.

Microdialysis technique and interventional radiology / 介入放射学杂志

Basic research in interventional radiology,including transcatheter artery perfusion especially,is progressing slowly due to lack of proper method.Microdialysis technique,a kind of accurate sampling technique in vivo,may help to solve the problem.Just as its name implies,microdialysis means tiny dialysis with advantages of authenticity,exactness and less error.Furthermore it has been applied widely and should be received with great attention and popularity.

Investigation on pharmacokinetics and bioavailabiUty of insulin dry powder inhalation / 中华内分泌代谢杂志

Objective To study the characteristics of pharmacokinetics and pharmacodynamics of insulin dry powder inhalation and its relative bioavailability as compared with subcutaneous injection of regular insulin. Methods In this open,single-center,randomized,two-period,cross-over,euglycemic glucose clamp study,18 healthy volunteers(14 men and 4 women),aged(24.9?1.7)years,with body mass index(20.6?1.2)kg/m~2, received the insulin dry powder inhalatin(80 U)or regular insulin(15 U)subcutaneous administration.The blood samples of this study at 0,20,30,40,50,60,70,80,90,100,110,120,135,150,165,180,195, 210,225,240,270,300,330,360,390,420,450 and 480 rain were taken for serum insulin measurement, meanwhile,glucose infusion rates(GIR)were determined per 5 minutes over a period of 8 hours.Results The C_(max)were(57.9?17.8 vs 114.5?29.7)mU/L(tested vs reference preparation),T_(max)were(46.7?45.6 vs 107.8?33.7)min,GIR_(max)were(3.35?0.98 vs 5.17?1.75)mg?kg~(-1)?min~(-1)and T_(GIRmax)were(88.3?17.0 vs 151.9?34.6)min.The relative bioavailability was(10.26?2.25)%,and the relative bioefficacy was(14.33?7.26)%.Conclusion The study shows that insulin dry powder inhalation is absorbed via lungs and its action sets in earlier than that of the regular insulin injected subcutaneously.These pharmacokinetie and pharmacodynamic data may provide a reliabe guide for further clinical trial.

PHARMACOKINETICES OF RANITIDINE IN NORMAL SUBJECTS / 重庆医科大学学报

The pharmacokinetics of ranitidine was studied with the method of high performance li-quid chromatography in 10 healthy volunteers. The subjects were given a single dose of ranitidine 150mg (one capsule) orally. The serum concentration-time curves showed that one-compartment open model could be used in all subjects. The equation of mean serum conccentration was and with following pharmacokinetic parameters: (hr), and There were no statistically significant differences between the, kinetic parameters of reported articles and ours

STUDY ON PHARMACOKINETICS OF TMPH FOR INTRAVENOUS INJECTION IN RAT / 中国药理学通报

Pharmacokinetics of Tetramethylpyrazine Hydrochloride (TMPH) in rat was studied by using Ultraviolet Spectrophotometry. After a bolus iv injection of TMPH 30 mg/kg to rat, the pharmacokinetic characteristics are found to fit a two-compartment open model. The Pharmacokinetic parameters are: t 1/2? = 0 .1441h, t 1/2? = l .6953h, K21=2.1850h-1, K10=0.8605h-1, K12= 2 .0723h-1, AUC = 83 .3660mg?L -1h, CL = 0.3599L?kg-1h-1, Yc =0 .4182L?kg-1 , Vss =0 .7975L.kg-1