Anise essential oil immobilized in chitosan microparticles: a novel bactericidal material for food protection.

Foodborne infections in humans are one of the major concerns of the food industries, especially for minimally processed foods (MPF). Thereby, the packaging industry applies free chlorine in the sanitization process, ensuring the elimination of any fecal coliforms or pathogenic microorganisms. However, free chlorine's propensity to react with organic matter, forming toxic compounds such as trihalomethanes and haloacetic acid. Therefore, the present work aimed to synthesize a novel organic biomaterial as an alternative to free chlorine. Chitosan microparticles were produced, with Pimpinella anisum (anise) essential oil immobilized in the biopolymer matrix (MPsQTO). The characterization of this biomaterial was done through GC-MS/MS, FT-IR, and SEM. Antimicrobial assays proved that the MPsQTO presented antibacterial activity for Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, and Bacillus subtilis at 300 µL mL-1 of concentration. The fluorescence microscope also showed the MPsQTO targets the cytoplasmatic membrane, which is responsible for cell death in the first minutes of contact. Studies with the mutant B. subtilis (amy::pspac-ftsZ-gfpmut1) and the Saccharomyces cerevisiae D7 also proved that the biomaterial did not affect the genetic material and did not have any mutagenic/carcinogenic effect on the cells. The sanitization assays with pumpkin MPF proved that the MPsQTO is more effective than free chlorine, increasing the shelf-life of the MPF. Consequently, the novel biomaterial proposed in this work is a promising alternative to traditional chemical sanitizers.

Oxidative stability and physicochemical changes of dark chocolates with essential oils addition.

This research aimed to evaluate the oxidative stability and rheological properties of dark chocolates with the addition of essential oils (EO) of Cymbopogon citratus, Pimpinella anisum, and Mintostachys mollis. For this purpose, before the inclusion in chocolates, the EO were chemically characterized to identify the most important volatile compounds. We added essential oils of P. anisum, C. citratus and M. mollis to dark chocolates (cocoa 70%) at doses of 10, 12 and 14 µL per 500 g, separately. These chocolates were evaluated for oxidative activity, hardness, microstructure, rheological and melting properties and antioxidant capacity. It was found that C. citratus EO (10 µL/500 g of chocolate) improve the oxidative stability of the chocolates at 90 days of storage at 25 °C (230 meq O2/kg), while higher concentrations promote lipid oxidation. The incorporation of essential oils improves the antioxidant capacity, likewise, it changes the rheological, thermal, and microstructural properties. Therefore, essential oils can improve the physicochemical characteristics of dark chocolates allowing greater stability in oxidative fat and thus increase the shelf life.

Influence of fennel in Japanese quail diet over egg quality and behavior aspects / Influência da erva-doce na dieta de codornas japonesas sobre a qualidade dos ovos e aspectos comportamentais

The objective of this research was to evaluate the effect of Pimpinella anisum (fennel) on the performance, stress, and quality of Japanese Quail eggs in the second laying cycle (73 to 77 weeks). One hundred sixty-eight female quails from Japanese sub-species (Coturnix coturnix japonica) at 73 weeks of age, divided and submitted to four treatments (0; 250; 500 and 750mg of fennel/kg of feed) were used in a randomized block design. (RBD), with seven replications and six birds per experimental plot, totaling 28 plots. Performance, behavioral, and egg quality parameters were evaluated. Bird performance was not influenced (p>0.05) by the treatments tested. The highest egg weight and albumen weight were found in birds fed 750 mg of fennel, while a higher albumen height was observed for the estimated dose of 669 mg (p<0,05). The estimated doses of 554.09 mg, 634.10 mg, and 613.10 mg of fennel were efficient in reducing agitated behaviors by riding, pecking, and stirring, respectively. However, non-aggressive variables were not influenced by the addition of fennel to the diet (p>0.05). The tonic immobility test had a decreasing linear effect (p<0.05), which indicates a lower time in seconds in tonic immobility to birds receiving 750 mg of fennel. Fennel added to the diet did not interfere with performance, but it influenced essential parameters related to egg quality and was able to alter characteristics related to bird behavior.(AU)

O objetivo desta pesquisa foi avaliar o efeito da Pimpinella anisum(erva-doce) sobre o desempenho, estresse e qualidade dos ovos de codornas japonesas no segundo ciclo de postura (73 a 77 semanas). Foram utilizadas 168 codornas fêmeas da subespécie japonesa (Coturnix coturnix japonica) com 73 semanas de idade, divididas e submetidas a quatro tratamentos (0; 250; 500 e 750mg de erva-doce/kg de ração), em delineamento em blocos casualizados (DBC), com sete repetições e seis aves por parcela experimental, totalizando 28 parcelas. Foram avaliados os parâmetros de desempenho, comportamentais e qualidade dos ovos. O desempenho das aves não foi influenciado (p>0,05) pelos tratamentos testados. O maior peso dos ovos e peso do albúmen foram encontrados nas aves alimentadas com 750 mg de erva-doce, enquanto a maior altura do albúmen foi observada pela dose estimada de 669 mg (p<0.05). As variáveis comportamentais agressivas foram consideradas diferentes entre os níveis analisados (p<0,05). As doses estimadas de 554,09 mg, 634,10 mg e 613,10 mg de erva-doce foram eficientes na redução dos comportamentos agitados montando, bicando e agitada, respectivamente. No entanto as variáveis não agressivas não foram influenciadas pela adição de erva-doce na dieta (p>0,05). O Teste de imobilidade tônica teve efeito linear (p<0,05) decrescente, que indica um menor tempo em segundos em imobilidade tônica para as aves que receberam 750 mg de erva-doce. A erva-doce adicionada à dieta não interfere no desempenho, porém melhorou à qualidade dos ovos e foi capaz de alterar características relacionadas ao comportamento das aves.(AU)

Influence of fennel in Japanese quail diet over egg quality and behavior aspects / Influência da erva-doce na dieta de codornas japonesas sobre a qualidade dos ovos e aspectos comportamentais

The objective of this research was to evaluate the effect of Pimpinella anisum (fennel) on the performance, stress, and quality of Japanese Quail eggs in the second laying cycle (73 to 77 weeks). One hundred sixty-eight female quails from Japanese sub-species (Coturnix coturnix japonica) at 73 weeks of age, divided and submitted to four treatments (0; 250; 500 and 750mg of fennel/kg of feed) were used in a randomized block design. (RBD), with seven replications and six birds per experimental plot, totaling 28 plots. Performance, behavioral, and egg quality parameters were evaluated. Bird performance was not influenced (p>0.05) by the treatments tested. The highest egg weight and albumen weight were found in birds fed 750 mg of fennel, while a higher albumen height was observed for the estimated dose of 669 mg (p0.05). The tonic immobility test had a decreasing linear effect (p<0.05), which indicates a lower time in seconds in tonic immobility to birds receiving 750 mg of fennel. Fennel added to the diet did not interfere with performance, but it influenced essential parameters related to egg quality and was able to alter characteristics related to bird behavior.

O objetivo desta pesquisa foi avaliar o efeito da Pimpinella anisum(erva-doce) sobre o desempenho, estresse e qualidade dos ovos de codornas japonesas no segundo ciclo de postura (73 a 77 semanas). Foram utilizadas 168 codornas fêmeas da subespécie japonesa (Coturnix coturnix japonica) com 73 semanas de idade, divididas e submetidas a quatro tratamentos (0; 250; 500 e 750mg de erva-doce/kg de ração), em delineamento em blocos casualizados (DBC), com sete repetições e seis aves por parcela experimental, totalizando 28 parcelas. Foram avaliados os parâmetros de desempenho, comportamentais e qualidade dos ovos. O desempenho das aves não foi influenciado (p>0,05) pelos tratamentos testados. O maior peso dos ovos e peso do albúmen foram encontrados nas aves alimentadas com 750 mg de erva-doce, enquanto a maior altura do albúmen foi observada pela dose estimada de 669 mg (p0,05). O Teste de imobilidade tônica teve efeito linear (p<0,05) decrescente, que indica um menor tempo em segundos em imobilidade tônica para as aves que receberam 750 mg de erva-doce. A erva-doce adicionada à dieta não interfere no desempenho, porém melhorou à qualidade dos ovos e foi capaz de alterar características relacionadas ao comportamento das aves.

Plant extracts with potential to control of two-spotted spider mite / Extratos vegetais com potencial de uso no controle do ácaro rajado

Tetranychus urticae Koch (Acari: Tetranychidae) is a species of great economic importance to Brazil. In its control, the main option is the use of synthetic miticides, which, if used indiscriminately, can contaminate the environment, farmers and consumers. In the search for control alternatives, this study aimed to evaluate the effect of plant extracts and essential oils on females of this pest. For this, T. urticae females, reared in laboratory, were sprayed in Potter tower. Fourteen plants were tested in the form of aqueous extracts (AE), hydroethanolic extracts (HE) or essential oils (EO). The experimental plot consisted of a Petri dish with a Canavalia ensiformis L. leaf disk containing five T. urticae females, using ten plates per treatment. Mortality evaluations were performed 24, 48, 72, 96 and 120 hours after spraying, recording the number of dead females on the disc and the number of dead females on the moistened cotton. We considered that females found on cotton were repelled by treatments. Matricaria chamomilla L. HE and Pimpinella anisum L. AE have acaricidal effect for females, providing above 83% of mortality, after 120 hours. Origanum vulgare L. HE causes at least 75% mortality after 24 hours. Also 120 hours after spraying, P. anisum HE and O. vulgare AE cause repellency of females above 16%, and A. absinthium HE exceeds 22% for this parameter. The results stimulate further researches to determine the optimum dose and evaluation of additional effects on the fertility of surviving females.(AU)

Tetranychus urticae Koch (Acari: Tetranychidae) é uma espécie de grande importância econômica para o Brasil. Em seu controle, a principal opção são os acaricidas sintéticos, que, se usados de forma indiscriminada, podem contaminar ambiente, produtores e consumidores. Na busca de alternativas de controle, este trabalho teve por objetivo avaliar o efeito de extratos vegetais e óleos essenciais sobre fêmeas dessa praga. Para isso, fêmeas de T. ­urticae criadas em laboratório foram pulverizadas em torre de Potter. Foram testadas 14 espécies vegetais, na forma de extratos aquosos (EA), hidroetanólicos (EH) ou óleos essenciais (OE). A parcela experimental consistiu de uma placa de Petri com um disco de folha de feijão-de-porco (Canavalia ­ensiformis L.) contendo cinco fêmeas de T. urticae, sendo utilizadas dez placas por tratamento. As avaliações de mortalidade foram realizadas 24, 48, 72, 96 e 120 horas após a pulverização, registrando-se o número de fêmeas mortas no disco e no algodão umedecido. Considerou-se que fêmeas encontradas no algodão foram repelidas pelos tratamentos. Matricaria chamomilla L. na forma de extrato hidroetanólico e Pimpinella anisum L. na forma de extrato aquoso possuem efeito acaricida para fêmeas proporcionando acima de 83% de mortalidade, após 120 horas da pulverização. Origanum ­vulgare L. EH causa no mínimo 75% de mortalidade após 24 horas. Também após 120 horas, P. anisum EH e O. vulgare EA causam repelência de fêmeas acima de 16% e A. absinthium EH ultrapassa 22% para esse parâmetro. Os resultados estimulam a realização de pesquisas futuras para determinação da dose ótima e avaliação de efeitos adicionais sobre a fertilidade das fêmeas sobreviventes.(AU)

Plant extracts with potential to control of two-spotted spider mite / Extratos vegetais com potencial de uso no controle do ácaro rajado

Tetranychus urticae Koch (Acari: Tetranychidae) is a species of great economic importance to Brazil. In its control, the main option is the use of synthetic miticides, which, if used indiscriminately, can contaminate the environment, farmers and consumers. In the search for control alternatives, this study aimed to evaluate the effect of plant extracts and essential oils on females of this pest. For this, T. urticae females, reared in laboratory, were sprayed in Potter tower. Fourteen plants were tested in the form of aqueous extracts (AE), hydroethanolic extracts (HE) or essential oils (EO). The experimental plot consisted of a Petri dish with a Canavalia ensiformis L. leaf disk containing five T. urticae females, using ten plates per treatment. Mortality evaluations were performed 24, 48, 72, 96 and 120 hours after spraying, recording the number of dead females on the disc and the number of dead females on the moistened cotton. We considered that females found on cotton were repelled by treatments. Matricaria chamomilla L. HE and Pimpinella anisum L. AE have acaricidal effect for females, providing above 83% of mortality, after 120 hours. Origanum vulgare L. HE causes at least 75% mortality after 24 hours. Also 120 hours after spraying, P. anisum HE and O. vulgare AE cause repellency of females above 16%, and A. absinthium HE exceeds 22% for this parameter. The results stimulate further researches to determine the optimum dose and evaluation of additional effects on the fertility of surviving females.(AU)

Tetranychus urticae Koch (Acari: Tetranychidae) é uma espécie de grande importância econômica para o Brasil. Em seu controle, a principal opção são os acaricidas sintéticos, que, se usados de forma indiscriminada, podem contaminar ambiente, produtores e consumidores. Na busca de alternativas de controle, este trabalho teve por objetivo avaliar o efeito de extratos vegetais e óleos essenciais sobre fêmeas dessa praga. Para isso, fêmeas de T. ­urticae criadas em laboratório foram pulverizadas em torre de Potter. Foram testadas 14 espécies vegetais, na forma de extratos aquosos (EA), hidroetanólicos (EH) ou óleos essenciais (OE). A parcela experimental consistiu de uma placa de Petri com um disco de folha de feijão-de-porco (Canavalia ­ensiformis L.) contendo cinco fêmeas de T. urticae, sendo utilizadas dez placas por tratamento. As avaliações de mortalidade foram realizadas 24, 48, 72, 96 e 120 horas após a pulverização, registrando-se o número de fêmeas mortas no disco e no algodão umedecido. Considerou-se que fêmeas encontradas no algodão foram repelidas pelos tratamentos. Matricaria chamomilla L. na forma de extrato hidroetanólico e Pimpinella anisum L. na forma de extrato aquoso possuem efeito acaricida para fêmeas proporcionando acima de 83% de mortalidade, após 120 horas da pulverização. Origanum ­vulgare L. EH causa no mínimo 75% de mortalidade após 24 horas. Também após 120 horas, P. anisum EH e O. vulgare EA causam repelência de fêmeas acima de 16% e A. absinthium EH ultrapassa 22% para esse parâmetro. Os resultados estimulam a realização de pesquisas futuras para determinação da dose ótima e avaliação de efeitos adicionais sobre a fertilidade das fêmeas sobreviventes.(AU)

Effect of various concentrations of an anise seed powder (pimpinella anisum l) Supplement on selected hematological and biochemical parameters of broiler chickens

The objective of the study was to investigate the influence of anise seed (Pimpinella anisum L.) powder supplemented to the drinking water on selected blood parameters of broilers. In total, 360 one-day-old Hubbard Classic chicks were randomly divided into four groups with three replicates of 30 birds each. Anise seed powder was added at 0, 500, 750, and 1000mg/L to the drinking water offered to the C (control), T1, T2, and T3 groups respectively. The supplement was supplied for 56 days. When broilers were 28 and 56 days old, blood samples were collected (30 birds per group) by brachial vein puncture to evaluate the cellular components of blood (RBC, WBC, Hgb, HCT, H/L). The following serum biochemical parameters were evaluated: CHOL, GLU, TP, albumin, globulins, Ca, P, TG, total lipids, UA, and creatinine, as well as AST and ALT enzyme activities. The statistical analysis indicated that the anise supplement significantly improved blood RBC, WBC, Hgb, HCT, TP, albumin, globulin, GLU, P, and Ca levels of broilers in groups T4, T3, and T2 compared with the control group on days 28 and 56, and on average. Also, T3, T2 and T1 presented lower H/L ratio and CHOL, TG, total lipids, creatinine, UA, AST and ALT serum levels compared with the control group. The highest inclusion levels of anise seed powder, i.e., 1000 and 750mg/L, had a stimulating effect on the physiological traits of the birds.(AU)

Effect of various concentrations of an anise seed powder (pimpinella anisum l) Supplement on selected hematological and biochemical parameters of broiler chickens

The objective of the study was to investigate the influence of anise seed (Pimpinella anisum L.) powder supplemented to the drinking water on selected blood parameters of broilers. In total, 360 one-day-old Hubbard Classic chicks were randomly divided into four groups with three replicates of 30 birds each. Anise seed powder was added at 0, 500, 750, and 1000mg/L to the drinking water offered to the C (control), T1, T2, and T3 groups respectively. The supplement was supplied for 56 days. When broilers were 28 and 56 days old, blood samples were collected (30 birds per group) by brachial vein puncture to evaluate the cellular components of blood (RBC, WBC, Hgb, HCT, H/L). The following serum biochemical parameters were evaluated: CHOL, GLU, TP, albumin, globulins, Ca, P, TG, total lipids, UA, and creatinine, as well as AST and ALT enzyme activities. The statistical analysis indicated that the anise supplement significantly improved blood RBC, WBC, Hgb, HCT, TP, albumin, globulin, GLU, P, and Ca levels of broilers in groups T4, T3, and T2 compared with the control group on days 28 and 56, and on average. Also, T3, T2 and T1 presented lower H/L ratio and CHOL, TG, total lipids, creatinine, UA, AST and ALT serum levels compared with the control group. The highest inclusion levels of anise seed powder, i.e., 1000 and 750mg/L, had a stimulating effect on the physiological traits of the birds.

Immunomodulatory effects of Pimpinella anisum L. (Aniseed) in Broiler Chicks against Newcastle disease and infectious bursal disease viruses / Efecto inmunomodulador de Pimpinella anisum L. (anís) en pollos de engorde contra la enfermedad de Newcastley la enfermedad viral de la bursitis infecciosa

Pimpinella anisum L. (Aniseed) is mostly used as an immune stimulant, growth promoter, antifungal, antibacterial in many countries for centuries. The aim of this study was to determine the immunomodulatory effect of aniseed against Newcastle Disease (ND) and infectious bursal disease (IBD) viruses. The immunomodulatory effect of aniseed against ND and IBD viruses were determined by modifying splenic cell migration inhibition assay and differential leukocyte count for cellular immunity. Haemagglutination inhibition and indirect haemagglutination were used for measurement of humoral immune response against ND and IBD viruses, respectively. The present study suggests that the aniseed addition to basal diet at the rate of 0.5 g/kg and 1 g/kg of feed had best immunomodulatory activity both for humoral and cellular immune responses. However, at higher doses aniseed had adverse effects. Aniseed possesses significant immunomodulatory activity when it is added at lower doses i.e., 0.5 g/kg and 1 g/kg.

Pimpinella anisum L. (Anís) se utiliza principalmente como un estimulante inmunológico, promotor del crecimiento, antifúngico, y antibacteriano, en muchos países durante siglos. El objetivo de este estudio fue determinar el efecto inmunomodulador de anís contra la enfermedad de Newcastle (ND) y la enfermedad de la bursitis infecciosa (IBD). El efecto inmunomodulador de anís contra los virus ND y e IBD se determinaron mediante la modificación del ensayo de inhibición de la migración de células del bazo y recuento diferencial de leucocitos de la inmunidad celular. La inhibición de la hemaglutinación y hemaglutinación indirecta se utilizaron para la medición de la respuesta inmune humoral contra el virus de ND e IBD, respectivamente. El presente estudio sugiere que la adición de anís a la dieta basal a la tasa de 0,5 g/kg y 1 g/kg de alimentación tuvo una mejor actividad inmunomoduladora tanto para las respuestas inmunes humorales como celulares. Sin embargo, a dosis más altas de anís tuvo efectos adversos. El anís posee una importante actividad inmunomoduladora cuando se añade en dosis más bajas, es decir, 0,5 g/kg y 1 g/kg.

Growth promoting effect of Pimpinella anisum (Aniseed) in broiler chickens / Efecto promotor del crecimiento de Pimpinella anisum (semilla de anis) en pollos broiler

The present study was conducted to evaluate growth promoting effect of Pimpinella anisum L. (aniseed) in broiler chickens in terms of body weight gain, organ weights, feed intake and feed conversion ratio (FCR). For this purpose, four levels (0.5, 1.0, 1.5 and 2.0 grams per kg feed) of aniseed powder were administered in feed from day 0 till the end of experiment (42 days of age). The body weight gain, feed intake and FCR were recorded on weekly basis. The birds showed good performance (better weight gain, better feed intake and FCR) when aniseed was fed in lower doses however, birds showed poor performance at higher levels of aniseed. Likewise, almost same pattern was observed for organ weights. This study suggests the good effect of aniseed on performance of broiler chickens at lower doses which suggests the further need of series experiments to find out the minimum safe levels of aniseed to be incorporated in broiler ration to get desirable results.

Se realizó el presente estudio para evaluar el efecto promotor del crecimiento de Pimpinella anisum L. (anís) en pollos de engorde en términos de ganancia de peso corporal, peso de los órganos, el consumo de alimento y conversión alimenticia (FCR). Para este propósito, cuatro niveles (0,5, 1,0, 1,5 y 2,0 gramos por kg de alimento) de anís en polvo se administraron en el pienso desde el día 0 hasta el final del experimento (42 días de edad). La ganancia de peso corporal, consumo de alimento y FCR fueron registrados en forma semanal. Las aves mostraron un buen rendimiento (mejor ganancia de peso, un mejor consumo de alimento y FCR) cuando el anís se alimentó en dosis más bajas, sin embargo, las aves mostraron malos resultados en los niveles más altos de anís. Del mismo modo, se observó casi el mismo patrón de peso de los órganos. Este estudio sugiere que el buen efecto del anís en el rendimiento de pollos de engorde en dosis más bajas que sugiere la necesidad adicional de experimentos de la serie para conocer los niveles de seguridad mínimos de anís que se incorporarán en la ración de pollos de engorde para obtener los resultados deseados.

Análisis de los principios activos de especies mexicanas de anís y evaluación de su actividad biológica sobre microorganismos patógenos / Analysis of the active principles of Mexican anise species and evaluation of their biological activity on pathogenic microorganisms

La planta de anís Pimpinella anisum (Saunf - Hindi) es una de las plantas medicinales más antiguas utilizadas por el hombre. Actualmente, esta planta tiene varios usos en la industria alimentaria como la especia, mientras que en la farmacopea se utiliza como expectorante en trastornos digestivos, como diurético suave y como repelente de insectos en el uso externo. En este trabajo se evaluó la actividad biológica del extracto metanólico de Pimpinella anisum sobre el crecimiento in vitro de Entamoeba histolytica HM1-IMSS bajo condiciones axénicas. Observamos que la inhibición del crecimiento de E. histolytica estaba en CI50 = 0.034 µg/mL. Los resultados confirmaron la actividad antiamobica del extracto metanólico de P. anisum.

Ação antimicrobiana in vitro de extratos glicólicos de Psidium guajava L., Syzygium cumini L e Pimpinella anisum L / In vitro antimicrobial action of extracts glicolics of Psidium guajava L., Syzygium cumini L and Pimpinella anisum L

Várias pesquisas vêm sendo desenvolvidas e direcionadas no descobrimento de novos agentes antimicrobianos provenientes de plantas, para serem aplicados em produtos farmacêuticos. O objetivo deste estudo foi avaliar in vitro a propriedade antimicrobiana de extratos glicólicos (75%) de Psidium guajava L. (goiabeira), Syzygium cumini L. (jambolão) e Pimpinella anisum L. (erva-doce) sobre cepas padrão de Candida albicans, Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, Escherichia coli e Bacillus atrophaeus (esporos). Foi determinada a concentração inibitória mínima (CIM) pelo método de microdiluição em caldo, seguindo-se de subcultivos e magar para determinar a concentração microbicida mínima (CMM). Os extratos apresentaram as seguintes CIM e CMM, respectivamente: goiabeira 25% e 50% para C. albicans, E. faecalis e E. coli; 12,5% e 25% para S. mutans, e, 3,12% e 6,25% para S. aureus; jambolão 25% e 50% para C. albicans, 12,5% e 25% para E. faecalis e S. aureus, e, 6,25% e 12,5% para E. coli e S. mutans; erva-doce 12,5% e 25% para C. albicans e S. mutans, 6,25% e 12,5% para E. faecalis e S. aureus, e 3,12% e 6,25% para E. coli. Conclui-se que, apesar da resistência apresentada por B. atrophaeus, os extratos utilizados demonstraram potencial antimicrobiano sobre os demais micro-organismos.

Fungi contamination in the chamomile, anis and mate teas / Contaminação fúngica em chás de camomila, erva-doce e erva-mate

Microbiological evaluation on stored products such as medicinal plants has been a matter of great concern for Food Safety due to the potentiality in producing mycotoxins by some fungal species. In the present paper, the isolation, quantification and identification of potentially mycotoxigenic fungal genera in the chamomile (Chamomilla recutita),anise (Pimpinella anisum) and mate tea (Ilex paraguariensis) tea-leaf samples are reported. The analyzed tea-leaf samples were purchased from stores located in the city of Curitiba, Paraná, Brazil, and they were evaluated in the forms of cool infusion (pouring cool water on the leaves), regular infusion (pouring hot liquid on the leaves) and leaves boiling process. In chamomile, mate tea and anis tea-leaf samples analyzed in infusion form, no significant differences on CFU/g values were showed. The cool infusion forms of chamomile and anis teas presented some significant differences, but not in mate tea cool infusion. The fungal genera isolated from analyzed samples were Aspergillus sp (35.9%); Penicillium sp (9.4%); Fusarium sp (0.21%); Rhizopus sp (11.5%), Ulocladium sp (18.4%)and Mycelia sterilia (6.84%). The potentially mycotoxigenic fungus Aspergillus sp was the most frequent in all of analyzed tea samples, followed by Penicillium sp e Fusarium sp. Considering that the potentially mycotoxigenic fungi remain in tea samples even after

A avaliação da qualidade microbiológica de produtos armazenados, utilizados como plantas medicinais, é fundamental para garantir a segurança alimentar, em função do potencial micotoxigênico apresentado por algumas espécies de fungos. No presente trabalho foram realizados o isolamento, a quantificação e a identificação de fungos potencialmente produtores de micotoxinas em amostras de chás de camomila (Chamomilla recutita L.), erva-doce (Pimpinella anisum L.) e erva-mate (Ilex paraguariensis St.-Hil.), adquiridas em estabelecimentos comerciais da cidade de Curitiba, PR, Brasil. As amostras foram analisadas nas formas de infusão fria, de infusão tradicional e decocção. Não houve diferenças significativas nos valores de UFC/g nas amostras de camomila, erva-mate e erva-doce analisadas na forma de infusão. Foram observadas diferenças significativas em amostras de camomila e erva-doce em forma de infusão fria em relação às demais formas de processamento de amostras , contudo essas não foram evidentes nas amostras de erva-mate. Os principais gêneros de fungos isolados das amostras analisadas foramAspergillus sp (35,9%); Penicillium sp (9,4%); Fusarium sp (0,21%); Rhizopus sp (11,5%), Ulocladium sp (18,4%) e Mycelia sterilia (6,84%). Entre os fungos potencialmente toxigênicos, o Aspergillus sp foi o mais frequente nos três tipos de chás, seguido de Penicillium sp e Fusarium sp. Consider

In vitro antimicrobial action of extracts glicolics of Psidium guajava L., Syzygium cumini L. and Pimpinella anisum L. / Ação antimicrobiana in vitro de extratos glicólicos de Psidium guajava L., Syzygium cumini L. e Pimpinella anisum L.

Several researches have been developed to search for new antimicrobial agents from plants, to be used in pharmaceutical products. The aim of this study was to evaluating in vitro antimicrobial properties of extracts glicolics (75%) of Psidium guajava L. (guava), Syzygium cumini L. (jambolan) and Pimpinella anisum L. (herbsweet) on standard strains of Candida albicans, Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, Escherichia coli and Bacillus atrophaeus (spores). During the experiment, the minimal inhibitory concentration (MIC) was determined by broth dilution method, following by subcultivation in agar in order to determine the minimal microbicide concentration (MMC). The extracts showed the following CIM and MMC, respectively: Psidium guajava L 25% and 50% for C. albicans, E. faecalis and E. coli, 12.5% and 25% for S. mutans, and 3.12% and6.25% for S. aureus; Syzygium cumini L. 25% and 50% for C. albicans, 12.5% and 25% for E. faecalis and S. aureus, and 6.25% and 12.5% for E. coli and S. mutans; Pimpinella anisum L -12.5% and 25% for C. albicans and S. mutans,6.25% and 12.5% for E. faecalis and S. aureus, and 3.12% and 6.25% for E. coli. It could be concluded that, despite the resistance by B. atrophaeus, the extracts used showed antimicrobial potential on the other microorganisms.

Várias pesquisas vêm sendo desenvolvidas e direcionadas no descobrimento de novos agentes antimicrobianos provenientes de plantas, para serem aplicados em produtos farmacêuticos. O objetivo deste estudo foi avaliar in vitro a propriedade antimicrobiana de extratos glicólicos (75%) de Psidium guajava L. (goiabeira), Syzygium cuminiL. (jambolão) e Pimpinella anisum L. (erva-doce) sobre cepas padrão de Candida albicans, Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, Escherichia coli e Bacillus atrophaeus (esporos). Foi determinada a concentração inibitória mínima (CIM) pelo método de microdiluição em caldo, seguindo-se de subcultivos emagar para determinar a concentração microbicida mínima (CMM). Os extratos apresentaram as seguintes CIM e CMM, respectivamente: goiabeira 25% e 50% para C. albicans, E. faecalis e E. coli; 12,5% e 25% para S. mutans, e,3,12% e 6,25% para S. aureus; jambolão 25% e 50% para C. albicans, 12,5% e 25% para E. faecalis e S. aureus, e,6,25% e 12,5% para E. coli e S. mutans; erva-doce 12,5% e 25% para C. albicans e S. mutans, 6,25% e 12,5% para E. faecalis e S. aureus, e 3,12% e 6,25% para E. coli. Conclui-se que, apesar da resistência apresentada por B. atrophaeus, os extratos utilizados demonstraram potencial antimicrobiano sobre os demais micro-organismos.