RESUMO
Objetivos: determinar os valores de normalidade dos componentes do plasma de coelhos da linhagem Nova Zelândia, convencionais, através de testes bioquímicos, comparando os resultados entre os sexos e entre os períodos, ao longo de exames realizados de 2008 a 2010. Material e Métodos: plasma de coelhos provenientes do Centro de Bioterismo da Faculdade de Medicina da Universidade de São Paulo foi testado por sistema de identificação-espectrofolometríca em analisador bioquímico semi-automatizado (Cobas Mira-Roche Diag System) e Kits comerciais (Bioclin/Biosystems e Labtest) padronizados para animais. Resultados: Houve uma variação significativ (p<0,05*) entre as concentrações de AST*, triglicérides e HDL, estando o primeiro e o terceiro aumentados entre as fêmeas e o terceiro, entre os machos. Na comparação dos resultados obtidos entre os anos, observou-se uma diferenciação para ALT nos machos e fêmeas (2008), aumento de LDH, triglicérides e amônia para machos (2009) e aumento de fósfoto, ferro, colesterol total, HDL e VLDL para fêmeas (2009). Conclusão: Foram obtidos os seguintes valores (Média ± erro padrão), machos e fêmeas, respectivamente, alanina aminotransferase (ALT) (U/L) 48,56 ± 8,32,41 ±16,91; aspartato aminotransferase (AST) (U/L)=46,81±15,0,56,8±17,09*; bilirrubina indireta (mg/dL)= 0,36±0,48,0,35; bilirrubina total (mg/dL)=1,02±1,32;2,0±1,17; bilirrubina direta (mg/dL)= 0,37±1,1,0,46±0,49; uréia (mg/dL)= 50,4±9,86,49,58±±10,02; creatina (mg/dL)= 0,37±1,1,0,46±0,94; uréia (mg/dL)=52,53±5,21,41,99±2,82, nitrogênio urêico sanguíneo (Bun-uréia) (mg/dL)= 6,87±0,76,7,82±1,16; cloretos (mEq/dL)= 78,14±8,42,83,04±8,33; glicose (mg/dL)= 104,63±13,37,110,36±12,04; magnésio (mg/dL)= 2,14±1,83,2,04±1,9; colesterol total (mg/dL)= 71,33±12,9,69,92±9,16; triglicerídeos (mg/dL)= 80,88±8,82,54,06±13,99*, lipoproteína de alta densidade (HDL) (mg/dL)= 10,32±2,26, 12,1±3,06, lipoproteína de baixa densidade (LDL) (mg/dL)= 31,73±16,69, 27±21,53, fosfatase alcalina (ALP) (U/L)= 133,1±12,13,140,96±12,77; proteínas totais (g/dL)= 6,07±1,39,6,53±1,25; albumína (g/dL)= 3,04±2,53,3,52±2,19; globulina (g/dL)= 1,83±2,57,2,9±2,02; cálcio (mg/dL)= 7,6±1,61,7,02±1,45; desidrogenase lática (LDH) (mg/dL)= 166,64±18,05, 162, 13±20,86. CEAU nº 251/11.
Objectives: To determine normal values of New Zealand rabbits plasma components conventional, by biochemical tests, comparing the results between gender and periods during tests conducted from 2008 to 2010. Material and Methods: Plasma from rabbits of the Faculdade de Medicina da USP (Universidade of São Paulo) fecilities was tested by spectrophotometric identification system for semi-automated biochemical analyzer (Cobas mira-Roche Diag System), and commercial kits (Bioclin/Biosystems and Labtest) standardized for animals. Results: There was a significant variation (p<0,05*) between the concentrations of AST* (aspartate aminotransferase), triglycerides and HDL (High Density Lipoprotein), with the first and third increased between the females and he third amon males. Comparing the results between years, there was a diference for ALT (alanine aminotransferase) in males and females (2008) , increased LDH (lactate denydiogenase), triglycerides and ammonia for males (2009) and increased phosphorus, iron, total cholesterol, HDL and VLDL (Very Low Density Lipoprotein) to females (2009). Conclusion: We obtained the following values (mean ±standard error), males and females, respectivelu: ALTT(I/L) 48.56±8.32, 41±16.91; AST (U/L) = 46.81±15.0, 56.8±17.09*: indirect bilirubin (mg/dL) = 0.36±0.48, 0.19±0.35; total bilirubin (mg/dL) = 1.02±17.09*; indirect bilirubin (mg/dL) = 0.55±0.23, 0.19±0.24; ammonia (mg/dL) = 50.4±9.86, 49.58±10.02; creatinine (mg/dL) - 0.37±1.1, 0.46±0.94; urea (md/dL) = (mg/dL) = 52.53±5.21, 45.99±2.82; blood urea nitrogen [Bun-urea] (mg/dL) = 6.87±0.76, 7.82±1.16; chloride (mEq dL) = 78.14±8.42, 83.04±8.33; phosphorus (mg/dL) = 5.04±0.95, 5.03±0.93; iron (,mg/dL) = 261.15±35.83, 214.26±45.7; glucose (mg/dL) = 104..63±13.37, 110.36±12.04, magnesium (mg/dL) = 2.14±1.83, 2.04±1.9; total cholesterol (mg/dL) = 71.33±12.9, 69.92±9.16; triglycerides (mg/dL) = 80.88±8.82, 54.06±13.99*; HDL (mg/dL) = 33.75±6.88, 39.43±6.49*; VLDL (mg/dL) = 10.32±2.26, 12.1±3.06; low density lipoprotein [LDL] (md/dL) = 31.73±16.69, 27.0±21.53; alkaline phsphatase [ALP] (U/L) = 133.1±12.13, 140.96±12.77; total protein (g/dL) = 6.07±1.39, 6.53±1.25; albumin (g/dL) = ± 3.04±2.53, 3.52±2.198/ globulin (g/dL) = 1.83±2.57, 2.9±2.02; calcium (mg/dL) = 7.6±1.61, 7.02±1.46; LDH (mg/dL) = 166.64±18.05, 162.13±20.86.
Assuntos
Animais , Coelhos , Manejo de Espécimes/métodos , Plasma/citologia , Análise Química do Sangue/estatística & dados numéricos , Coelhos/classificaçãoRESUMO
Objetivos: determinar os valores de normalidade dos componentes do plasma de coelhos da linhagem Nova Zelândia, convencionais, através de testes bioquímicos, comparando os resultados entre os sexos e entre os períodos, ao longo de exames realizados de 2008 a 2010. Material e Métodos: plasma de coelhos provenientes do Centro de Bioterismo da Faculdade de Medicina da Universidade de São Paulo foi testado por sistema de identificação-espectrofolometríca em analisador bioquímico semi-automatizado (Cobas Mira-Roche Diag System) e Kits comerciais (Bioclin/Biosystems e Labtest) padronizados para animais. Resultados: Houve uma variação significativ (p<0,05*) entre as concentrações de AST*, triglicérides e HDL, estando o primeiro e o terceiro aumentados entre as fêmeas e o terceiro, entre os machos. Na comparação dos resultados obtidos entre os anos, observou-se uma diferenciação para ALT nos machos e fêmeas (2008), aumento de LDH, triglicérides e amônia para machos (2009) e aumento de fósfoto, ferro, colesterol total, HDL e VLDL para fêmeas (2009). Conclusão: Foram obtidos os seguintes valores (Média ± erro padrão), machos e fêmeas, respectivamente, alanina aminotransferase (ALT) (U/L) 48,56 ± 8,32,41 ±16,91; aspartato aminotransferase (AST) (U/L)=46,81±15,0,56,8±17,09*; bilirrubina indireta (mg/dL)= 0,36±0,48,0,35; bilirrubina total (mg/dL)=1,02±1,32;2,0±1,17; bilirrubina direta (mg/dL)= 0,37±1,1,0,46±0,49; uréia (mg/dL)= 50,4±9,86,49,58±±10,02; creatina (mg/dL)= 0,37±1,1,0,46±0,94; uréia (mg/dL)=52,53±5,21,41,99±2,82, nitrogênio urêico sanguíneo (Bun-uréia) (mg/dL)= 6,87±0,76,7,82±1,16; cloretos (mEq/dL)= 78,14±8,42,83,04±8,33; glicose (mg/dL)= 104,63±13,37,110,36±12,04; magnésio (mg/dL)= 2,14±1,83,2,04±1,9; colesterol total (mg/dL)= 71,33±12,9,69,92±9,16; triglicerídeos (mg/dL)= 80,88±8,82,54,06±13,99*, lipoproteína de alta densidade (HDL) (mg/dL)= 10,32±2,26, 12,1±3,06, lipoproteína de baixa densidade (LDL) (mg/dL)= 31,73±16,69, 27±21,53, fosfatase alcalina (ALP) (U/L)= 133,1±12,13,140,96±12,77; proteínas totais (g/dL)= 6,07±1,39,6,53±1,25; albumína (g/dL)= 3,04±2,53,3,52±2,19; globulina (g/dL)= 1,83±2,57,2,9±2,02; cálcio (mg/dL)= 7,6±1,61,7,02±1,45; desidrogenase lática (LDH) (mg/dL)= 166,64±18,05, 162, 13±20,86. CEAU nº 251/11.(AU)
Objectives: To determine normal values of New Zealand rabbits plasma components conventional, by biochemical tests, comparing the results between gender and periods during tests conducted from 2008 to 2010. Material and Methods: Plasma from rabbits of the Faculdade de Medicina da USP (Universidade of São Paulo) fecilities was tested by spectrophotometric identification system for semi-automated biochemical analyzer (Cobas mira-Roche Diag System), and commercial kits (Bioclin/Biosystems and Labtest) standardized for animals. Results: There was a significant variation (p<0,05*) between the concentrations of AST* (aspartate aminotransferase), triglycerides and HDL (High Density Lipoprotein), with the first and third increased between the females and he third amon males. Comparing the results between years, there was a diference for ALT (alanine aminotransferase) in males and females (2008) , increased LDH (lactate denydiogenase), triglycerides and ammonia for males (2009) and increased phosphorus, iron, total cholesterol, HDL and VLDL (Very Low Density Lipoprotein) to females (2009). Conclusion: We obtained the following values (mean ±standard error), males and females, respectivelu: ALTT(I/L) 48.56±8.32, 41±16.91; AST (U/L) = 46.81±15.0, 56.8±17.09*: indirect bilirubin (mg/dL) = 0.36±0.48, 0.19±0.35; total bilirubin (mg/dL) = 1.02±17.09*; indirect bilirubin (mg/dL) = 0.55±0.23, 0.19±0.24; ammonia (mg/dL) = 50.4±9.86, 49.58±10.02; creatinine (mg/dL) - 0.37±1.1, 0.46±0.94; urea (md/dL) = (mg/dL) = 52.53±5.21, 45.99±2.82; blood urea nitrogen [Bun-urea] (mg/dL) = 6.87±0.76, 7.82±1.16; chloride (mEq dL) = 78.14±8.42, 83.04±8.33; phosphorus (mg/dL) = 5.04±0.95, 5.03±0.93; iron (,mg/dL) = 261.15±35.83, 214.26±45.7; glucose (mg/dL) = 104..63±13.37, 110.36±12.04, magnesium (mg/dL) = 2.14±1.83, 2.04±1.9; total cholesterol (mg/dL) = 71.33±12.9, 69.92±9.16; triglycerides (mg/dL) = 80.88±8.82, 54.06±13.99*; HDL (mg/dL) = 33.75±6.88, 39.43±6.49*; VLDL (mg/dL) = 10.32±2.26, 12.1±3.06; low density lipoprotein [LDL] (md/dL) = 31.73±16.69, 27.0±21.53; alkaline phsphatase [ALP] (U/L) = 133.1±12.13, 140.96±12.77; total protein (g/dL) = 6.07±1.39, 6.53±1.25; albumin (g/dL) = ± 3.04±2.53, 3.52±2.198/ globulin (g/dL) = 1.83±2.57, 2.9±2.02; calcium (mg/dL) = 7.6±1.61, 7.02±1.46; LDH (mg/dL) = 166.64±18.05, 162.13±20.86.(AU)