Behavioral trade-offs and multitasking by elk in relation to predation risk from Mexican gray wolves.

Predator non-consumptive effects (NCE) can alter prey foraging time and habitat use, potentially reducing fitness. Prey can mitigate NCEs by increasing vigilance, chewing-vigilance synchronization, and spatiotemporal avoidance of predators. We quantified the relationship between Mexican wolf (Canis lupus baileyi) predation risk and elk (Cervus canadensis) behavior. We conducted behavioral observations on adult female elk and developed predation risk indices using GPS collar data from Mexican wolves, locations of elk killed by wolves, and landscape covariates. We compared a priori models to determine the best predictors of adult female behavior and multitasking. Metrics that quantified both spatial and temporal predation risk were the most predictive. Vigilance was positively associated with increased predation risk. The effect of predation risk on foraging and resting differed across diurnal periods. During midday when wolf activity was lower, the probability of foraging increased while resting decreased in high-risk areas. During crepuscular periods when elk and wolves were most active, increased predation risk was associated with increased vigilance and slight decreases in foraging. Our results suggest elk are temporally avoiding predation risk from Mexican wolves by trading resting for foraging, a trade-off often not evaluated in behavioral studies. Probability of multitasking depended on canopy openness and an interaction between maternal period and predation risk; multitasking decreased prior to parturition and increased post parturition in high-risk areas. Openness was inversely related to multitasking. These results suggest adult female elk are altering the type of vigilance used depending on resource availability/quality, current energetic needs, and predation risk. Our results highlight potentially important, but often-excluded behaviors and trade-offs prey species may use to reduce the indirect effects of predation and contribute additional context to our understanding of predator-prey dynamics.

Influence of the number of hosts and the risk of predation on the foraging behavior of the parasitoid Eretmocerus eremicus.

BACKGROUND: Parasitoids are natural enemies that can modify their behaviors as they search for hosts based on the characteristics of the sites in which they forage. Theoretical models predict that a parasitoid will stay for longer periods in high-quality sites or patches than in those of low quality. In turn, patch quality may be linked to factors such as the number of hosts and the risk of predation. In the present study, we sought to determine if the factors of the number of hosts, risk of predation, and their interaction influence the foraging behavior of the parasitoid Eretmocerus eremicus (Hymenoptera: Aphelinidae) as theory predicts. To do this, we evaluated different parameters of parasitoid foraging behavior, such as residence time, number of oviposition events, and attacks, in sites of different patch quality. RESULTS: Our results show that when the factors, number of hosts, and, risk of predation, were evaluated separately, E. eremicus resided for longer periods and oviposited more frequently in patches with a high number of hosts and low risk of predation than in other patches. However, when both of these factors were combined, only the number of hosts influenced some aspects of the foraging behavior of this parasitoid, such as the number of oviposition events and attacks. CONCLUSION: For some parasitoids like E. eremicus, theoretical predictions may be fulfilled when patch quality is linked to the number of hosts but will not be fully satisfied when patch quality is related to the risk of predation. Furthermore, at sites with different combinations of host numbers and risk of predation, host number appears to be more critical than predation risk. These results suggest that the performance of the parasitoid E. eremicus to control whiteflies will be mainly mediated by the levels of whitefly infestation and, to a small extent, by the risk of predation to which the parasitoid is subjected. © 2023 Society of Chemical Industry.

Risk effects cascade up to an obligate scavenger.

The effects of predation risk on prey populations have been studied extensively; yet, how risk is manifested in a trophically linked guild-scavengers-has been overlooked. Risk could be particularly consequential for obligate scavengers that are vulnerable while foraging and rely on carrion provisioned by, and shared with, apex predators. We investigated whether Andean condors (Vultur gryphus) respond to predation risk in a landscape where the main source of carrion are camelids killed by pumas (Puma concolor). We hypothesized that condors would exhibit different behavioral responses to predation risk while they search, encounter, and exploit carrion. We explored condor habitat selection while flying by tracking nine birds with satellite transmitters and monitored via camera traps 41 natural carcasses and 25 experimental carrion stations. We found that condors searched for carrion in areas with a high probability of occurrence of puma kills. However, condors avoided exploiting carrion in areas featuring tall vegetation and steep slopes-selected by pumas to stalk prey-suggesting that condors manage risk primarily through the identification of safe foraging sites prior to landing. Our finding that condors avoided foraging near stalking cover for pumas highlights the importance of risk effects beyond predator-prey interactions, particularly for obligate scavengers.

Immediate predation risk alters the relationship between potential and realised selection on male traits in the Trinidad guppy Poecilia reticulata.

Imminent predation risk affects mating behaviours in prey individuals in a multitude of ways that can theoretically impact the strength of sexual selection, as well as its operation on traits. However, empirical studies of the effects of imminent predation risk on sexual selection dynamics are still scarce. Here we explore how perceived predation affects: (1) the relationship between the opportunity for selection and the actual strength of selection on male traits; and (2) which traits contribute to male fitness and the shape of selection on these traits. We simulate two consecutive reproductive episodes, under control conditions and perceived predation risk using experimental populations of Trinidad guppies. The opportunity for selection is higher under predation risk compared to the control condition, but realised selection on traits remains unaffected. Pre- and postcopulatory traits follow complex patterns of nonlinear selection in both conditions. Differences in selection gradients deviate from predictions based on evolutionary and non-lethal effects of predation, the most notable being strong disruptive selection on courtship rate under predation risk. Our results demonstrate that sexual selection is sensitive to imminent predation risk perception and reinforce the notion that both trait-based and variance-based metrics should be employed for an informative quantification.

Habituation in anuran tadpoles and the role of risk uncertainty.

The ability to learn in the context of predation allows prey to respond to threats by adjusting their behavior based on specific information acquired from their current environment. Habituation is a process that allows animals to adapt to environmental changes. Very little is known about habituation in wild animals in general and there are no studies on habituation in anuran tadpoles in particular. Here, we performed three experiments to investigate the behavioral response of predator naïve Pleurodema thaul tadpoles to repeated stimulation with two predation risk cues (injured conspecific and predator fed cues) which a priori provide different information regarding risk. Experiment 1 showed that P. thaul tadpoles habituate the antipredator response when undergo predation risk chemical cues from injured conspecific and that response is long term. Experiment 2 showed that P. thaul tadpoles did not habituate their antipredator response when exposed to cues derived from an event of nymph odonate preying on P. thaul tadpoles (predator fed cues). Experiment 3 specifically evaluated the risk imposed by each of the risk cues used in Experiment 1 and Experiment 2 and showed that the degree of perceived risk in tadpoles appear to be similar in a single experience with any risk stimuli. We suggest that the behavioral habituation of tadpoles in the context of predation could be modulated by the level of uncertainty associated with risk stimuli.

The role of hunting on Sapajus xanthosternos' landscape of fear in the Atlantic Forest, Brazil.

Habitats with spatial variation in food availability, predation risk, and hunting pressure allow us to study how animals resolve the trade-off between food searching and predator avoidance. We investigated the influence of food availability, predation risk, and the perceived predation risk on habitat use by a primate living under high hunting pressure, the yellow-breasted capuchin monkeys, Sapajus xanthosternos, at Una Biological Reserve (ReBio Una). We hypothesized that the hunting pressure occurring in the capuchins' home range would favor predator avoidance to the detriment of searching for food. We characterized a set of covariates related to resource availability (fruit and invertebrate biomasses, feeding on dispersed and clumped food items, sleeping sites), perceived predation risk (alarm calls given to terrestrial and aerial predators, silent group movement, and vigilance behavior), and actual predation risk (evidence of hunting) and estimated their effects on how one group of capuchin monkeys uses its habitat. The group divides its time among three major forest types within their home range: agroforest, mature, and secondary. Our results suggest that the actual and perceived risk of hunting by humans, as well as the perceived predation risk by both terrestrial and aerial predators, were significant determinants of capuchin monkeys' space use. Yellow-breasted capuchin monkeys' space use was negatively related to the risk of hunting by humans (actual evidence and silent behavior), the perceived risk of predation by both aerial and terrestrial predators, and the presence of sleeping sites. Capuchin monkeys' use of space was not related to the biomass of fruits in the habitat, and the biomass of invertebrates had a very low positive effect. We confirmed our prediction that in a habitat with high hunting pressure, the risk of predation, both perceived and actual, had a more significant impact on how yellow-breasted capuchins used space than did food availability.

Community-wide consequences of nonconsumptive predator effects on a foundation species.

Predators can exert nonconsumptive effects (NCEs) on prey, which often take place through prey behavioural adjustments to minimise predation risk. As NCEs are widespread in nature, interest is growing to determine whether NCEs on a prey species can indirectly influence several other species simultaneously, thus leading to changes in community structure. In this study, we investigate whether a predator can exert NCEs on a foundation species and indirectly affect community structure. Through laboratory experiments, we first tested whether the predatory marine snail Acanthina monodon exerts negative NCEs on larviphagy (consumption of pelagic larvae) and phytoplankton filtration rates of the mussel Perumytilus purpuratus, an intertidal foundation species. These hypotheses stem from the notion that mussels may decrease feeding activities in the presence of predator cues to limit detection by predators. Afterwards, a field experiment tested whether the presence of A. monodon near mussel beds leads to higher colonisation rates of invertebrates that reproduce through pelagic larvae (expected under a lower larviphagy in P. purpuratus) and to a lower algal biomass on P. purpuratus shells (expected under a lower metabolite excretion in the mussels), thereby changing the community structure of the species typically found in P. purpuratus beds. The laboratory experiments revealed that waterborne cues from A. monodon limit the larviphagy and filtration rates of P. purpuratus. In turn, the field experiment showed that A. monodon cues led to greater abundances of barnacles and bivalves and a lower algal biomass in P. purpuratus beds, thus altering community structure. Overall, this study shows that a predator can indirectly affect community structure through NCEs on an invertebrate foundation species. As invertebrate foundation species are ubiquitous worldwide, understanding predator NCEs on these organisms could help to better understand community regulation in systems structured by such species.

The effect of pelage, background, and distance on predator detection and the evolution of primate color vision.

Primates' predators, such as carnivore mammals, usually rely on camouflage to increase proximity to prey and start a predatory attempt. Camouflage depends not only on the color pattern presented by a predator's pelage but also on the background scene in which the hunting takes place. Another factor that influences camouflage effectiveness is prey's color vision since a given camouflage strategy might not work for all visual phenotypes. Still, little research has been made on the effect of primate visual phenotype on predator detection. Here, we investigate the effects of natural pelages, background scenarios, visual phenotypes, and detection distances on predator detection. We used photographs of taxidermized carnivores (ocelots, cougars, and lesser grisons) as detection stimuli, taken in three different natural scenarios (forest, savanna, and grassland), and at two viewing distances (near and far). On a touchscreen monitor, sets of four images (only one containing a hidden animal) were randomly presented to 39 human males (19 dichromats and 20 trichromats). We found that trichromats, when compared to dichromats, present a lower latency and a higher accuracy of carnivore detection for some conditions tested. We also found that pelage color, background scenario, and detection distance interact to influence the effectiveness of camouflage. Our results suggest that trichromacy might be even more advantageous for carnivore detection than thought before, since it facilitates detection of mammals with diverse pelage colorations, in environments with different phytophysiognomies, and at longer distances. We also propose that the higher rates of dichromacy found in modern human societies could have resulted from a relaxation in predation.

For emergency only: terrestrial feeding in Coimbra-Filho's titis reflects seasonal arboreal resource availability.

Terrestriality in Platyrrhine primates is primarily associated with low arboreal resource availability, low predation risk when on the ground and increased contact time with human observers. To test the relationship between these variables and ground use frequency, we studied a group of endangered Coimbra-Filho's titi monkeys (Callicebus coimbrai) in a 14-ha forest fragment in north-eastern Brazil. Terrestriality data were collected on a monthly basis (33 months) using scan sampling procedures from July 2008 to July 2012. Overall, Coimbra-Filho's titi monkeys were recorded during 0.6% of observation time (113 out of 18,164 scans) on the ground. Most of the time on the ground was spent feeding on young leaves (71 records) and the least amount of time on fruits (14 records). Availability of arboreal foods, rainfall, and time of contact with human observers did not influence overall terrestrial behaviour (ground use). However, the timing and nature of the monkeys' terrestrial feeding was strongly related to the absence of arboreal fruit (ß-estimate = -3.078) and young leaf (ß-estimate = -3.515) food resources. We suggest that terrestrial feeding by Coimbra-Filho's titi monkeys could be an adaptation to low arboreal fruit availability and the exploitation of alternative food resources.

FOOD SELECTION AND USE OF SPACE BY Didelphis pernigra (DIDELPHIDAE: MAMMALIA) IN A SUBURBAN ANDEAN ENVIRONMENT / Selección de alimento y uso del espacio por Didelphis pernigra (Didelphidae: Mammalia) en un ambiente uburbano andino

ABSTRACT Cities have grown throughout the Andes and we know little about the ecology of those species that tolerate them, limiting our options to do conservation. We applied optimal foraging theory to examine the behavior of the Andean White-eared Opossum (Didelphispernigra), in a suburban area in Bogotá, Colombia. We used the giving-up density technique, which uses the amount of food left in a feeding patch, to evaluate whether the opossum's foraging costs were affected by the height of food from the ground, and the quality and quantity of food. We also evaluated whether the spatial heterogeneity of the study site affected the opossum's foraging. We used an artificial feeding patch to test these ideas. When food was either concentrated and, in less amount (concentrated food) or diluted and more amount (diluted food), the opossums preferred to forage at 2 m than at 0.5 m, and they showed a preference for concentrated food at 0.5 m, but not at 2 m. The opossums' habitat use was affected by the spatial heterogeneity at the study site and animals preferred foraging along metal fences than on live fences made of trees. When a cable allowed connection between the metallic and live fences, the value of food patches at the live fence appeared to increase. Thus, although the opossums need resources associated with natural environments, our results suggest that there are human modifications that can benefit them, such as those that reduce the risk of predation and favor their mobility in suburban environments.

RESUMEN En los Andes las ciudades han crecido y sabemos poco sobre la ecología de aquellas especies que toleran dicho crecimiento, limitando las opciones para su apropiada conservación. Aplicamos la teoría de uso óptimo de parches para examinar el comportamiento de forrajeo del fara (Didelphispernigra) en un área suburbana de Bogotá, Colombia. Utilizamos la técnica de densidad de abandono, que usa la cantidad de alimento dejada en un parche, para evaluar si los costos de forrajeo de este marsupial fueron afectados por la altura de los parches desde el suelo, así como la calidad y cantidad de alimento. Igualmente, evaluamos el efecto de la heterogeneidad espacial del área de estudio sobre el forrajeo del fara. Usamos un parche alimentario artificial con alimento líquido para evaluar estas hipótesis. Cuando el alimento era concentrado y en menor cantidad (concentrado) o diluido y en mayor cantidad (diluido), los faras prefirieron forrajear a 2 m, y prefirieron el alimento concentrado sobre el diluido a 0.5 m, pero no a 2 m. El uso del hábitat del fara fue afectado por la heterogeneidad espacial en el sitio de estudio y ellos prefirieron forrajear a lo largo de cercas metálicas, más que sobre cercas vivas. Cuando un cable permitió la conexión entre las cercas metálicas y vivas, el valor de algunos parches en la cerca viva pareció aumentar. Aunque los faras necesitan recursos asociados con ambientes naturales, nuestros resultados sugieren que las modificaciones humanas, como las que reducen el riesgo de depredación y favorecen su movilidad, pueden beneficiarlos.

Increased terrestriality in a Neotropical primate living on islands with reduced predation risk.

An arboreal lifestyle is thought to be central to primate origins, and most extant primate species still live in the trees. Nonetheless, terrestrial locomotion is a widespread adaptation that has arisen repeatedly within the primate lineage. The absence of terrestriality among the New World monkeys (Platyrrhini) is thus notable and raises questions about the ecological pressures that constrain the expansion of platyrrhines into terrestrial niches. Here, we report the results of a natural experiment, comparing patterns of terrestrial behavior in white-faced capuchin monkeys (Cebus capucinus imitator) living on two islands off the Pacific coast of Panama that lack mammalian predators (island sites) with the behavior of capuchins at three sites in central Panama with more intact predator communities (mainland sites). Surveys with camera traps revealed increased terrestriality in island vs. mainland sites. Capuchin detection rates were higher, the range of party sizes observed was larger, and individuals engaged in a wider range of terrestrial behaviors on the islands lacking mammalian predators. Furthermore, females carrying infants were frequently photographed on the ground at the island sites, but never at the mainland sites. These findings support the long-standing hypothesis that predators constrain the exploitation of terrestrial niches by primates. These results are also consistent with the hypothesis that arboreal locomotion imposes costs that primates will avoid by walking on the ground when predation risk is low.

Differential antipredatory responses in the tuco-tuco (Ctenomys talarum) in relation to endogenous and exogenous changes in glucocorticoids.

Glucocorticoids participate in the behavioral and physiological responses generated under stressful circumstances coming from different sources-physical and/or psychological. In mammals, the increases of these hormones are mediated by the activation of the hypothalamic-pituitary-adrenal axis. This response occurs after exposure to novel and unpredictable situations that lead to the loss of homeostasis, for example, a direct encounter with predators or their cues. However, the relationship between the physiological and behavioral responses is still a complex issue in vertebrates. We evaluate the effects of an experimental manipulation of glucocorticoid levels on the generation of the behavioral and physiological response to stress by predation in the subterranean rodent C. talarum. We found that when tuco-tucos encountered predator cues-fur odor, and largely, immobilization-they responded physiologically by secreting cortisol. This response was accompanied by an associated behavioral response. However, when the increase in plasma cortisol originated exogenously by the injection of cortisol, a behavioral response was not observed. Finally, inhibition of glucocorticoids' synthesis was effective in weakening the behavioral effects produced by immobilization. In conclusion, in tuco-tucos, predator cues act as stress factors that trigger differential increases in plasma cortisol and a behavioral response associated with the appearance of anxiety states.

Information about Predators Varies across an Amazonian Rain Forest as a Result of Sentinel Species Distribution.

Information about predation risk is of fundamental value in biological communities. Because many prey species have shared predators, eavesdropping on other species' alarms is a widely recognized mechanism underlying the formation of mixed-species groups. However, information transfer may vary both across and within groups because some species provide higher-quality information about predators than others. We tested this phenomenon in Amazonian understory mixed-species flocks of birds in which two sentinel species-the bluish-slate antshrike (Thamnomanes schistogynus) and the dusky-throated antshrike (Thamnomanes ardesiacus)-occupy different habitats and provide alarm calls that are used by eavesdropping flock mates. In a playback experiment, two associate species responded significantly more strongly to alarm calls from the same sentinel species, reflecting the greater reliability of information about predator threats that could affect survival and habitat choice. Our work provides evidence of a repeated asymmetry across space in the available information about threats.

Are all patches worth exploring? Foraging desert birds do not rely on environmental indicators of seed abundance at small scales.

BACKGROUND: Consumers should show strong spatial preferences when foraging in environments where food availability is highly heterogeneous and predictable. Postdispersal granivores face this scenario in most arid areas, where soil seed bank abundance and composition associates persistently with vegetation structure at small scales (decimetres to metres). Those environmental features should be exploited as useful pre-harvest information, at least to avoid patches predicted to be poor. However, we did not find the expected spatial association in the algarrobal of the central Monte desert by observing foraging seed-eating birds, a field technique influenced by how much they exploit visited patches. In this work we tested if the first stage of foraging by granivorous birds (patch visit, encounter or exploration) is positively associated with environmental indicators of patch quality by recording the removal of single seeds from 300 scattered experimental devices during seasonal trials. Spatial selectivity was analysed by comparing the structural characteristics of used vs. available microhabitats, and evaluated against bottom-up and top-down hypotheses based on our previous knowledge on local seed bank abundance, composition and dynamics. Their foraging activity was also explored for spatial autocorrelation and environmental correlates at bigger scales. RESULTS: Postdispersal granivorous birds were less selective in their use of foraging space than expected if microhabitat appearance were providing them relevant information to guide their search for profitable foraging patches. No microhabitat type, as defined by their vegetation structure and soil cover, remained safe from bird exploration. Analyses at bigger temporal and spatial scales proved more important to describe heterogeneity in seed removal. CONCLUSIONS: Closeness to tall trees, probably related to bird territoriality and reproduction or to their perception of predation risk, seemed to determine a first level of habitat selection, constraining explorable space. Then, microhabitat openness (rather than seed abundance) exerted some positive influence on which patches were more frequently visited among those accessible. Selective patterns by birds at small scales were closer to our predictions of a top-down spatial effect, with seed consumption creating or strengthening (and not responding to) the spatial pattern and dynamics of the seed bank.

Chemically induced plasticity in early life history of Palaemon argentinus: are chemical alarm cues conserved within palaemonid shrimps?

Most aquatic animals use infochemicals from both conspecifics and heterospecifics to assess local predation risks and enhance predator detection. Released substances from injured conspecifics and other species (chemical alarm cues) are reliable cues to indicate an imminent danger in a specific habitat and often mediate the development of inducible defenses. Amphibian and fish embryos have been shown to acquire this information while at the embryonic stage of development, in relation to the developing nervous system and sensory development. With the exception of Daphnia, there is no information on chemically mediated responses to alarm cues in embryos of any crustacean groups. Therefore, we tested whether embryo exposure to chemical cues simulating predation on conspecifics or heterospecifics (closely related, non-coexisting species), or a mixture of both, alters embryonic developmental time, size and morphology of the first larval instar in Palaemon argentinus (Crustacea: Decapoda). Embryonic exposure to chemical alarm cues from conspecifics shortened the embryonic developmental time and elicited larger larvae with a longer rostrum. Rostrum length of the first larval instar changed independently of their size, thus elongated rostra can be considered a defensive feature. Embryonic developmental time was not altered by chemical alarm cues from either heterospecifics or the mixed cues treatment; however, exposure to these cues resulted in larger larvae compared with the control group. Chemically induced morphological plasticity in larvae in response to alarm cues from con- and heterospecifics suggests that such cues are conserved in palaemonids shrimps, providing embryos with an innate recognition of heterospecific alarm cues as predicted by the phylogenetic relatedness hypothesis.

"Armed to the teeth": The multiple ways to survive insecticidal and predatory challenges in Aedes aegypti larvae.

Environmental pollutants, such as insecticides, can alter the equilibrium of aquatic ecosystems, particularly those closely located to human occupations. The use of such anthropogenic compounds frequently results in the selection of resistant individuals. However, how the underlying insecticide resistance mechanisms interplay with the abilities of the resistant individuals to cope with other environmental challenges (e.g., predators) has not received adequate attention. Here, we evaluated whether resistance to pyrethroid insecticides in larvae of the yellow fever mosquito, Aedes aegypti (Diptera: Culicidae), would affect their abilities to survive other environmental challenges. We assessed the susceptibilities of the pyrethroid-resistant larvae to other insecticides (i.e., the oxadiazine indoxacarb and juvenile hormone mimic pyriproxyfen) and determined the activities of potential detoxification enzymes. Finally, we also recorded potential alterations in larva swimming behavior in the presence of predators, such as the water bug Belostoma anurum (Hemiptera: Belostomatidae). Our results revealed that high pyrethroid resistance was associated with moderate resistance to the other two insecticides. Furthermore, this multiple resistance was associated with higher detoxification activity by glutathione-S-transferases and general esterases. Interestingly, in comparison with insecticide-susceptible larvae, the pyrethroid-resistant larvae not only swam for longer periods and distances, but also took longer to be captured by B. anurum nymphs. Collectively, our findings revealed increased abilities to survive natural environmental challenges (e.g., predatory attacks) in mosquito larvae that express physiological and behavioral changes associated with multiple resistance to insecticides.

Combined effects of predator odor and alarm substance on behavioral and physiological responses of the pearl cichlid.

Several fish species trigger defensive behavioral responses when exposed to chemical cues that indicate predation risk. In these situations, physiological adjustments are also necessary to prepare the organism for a defensive response and increase survival odds. Chemical cues may be derived from predator odor or injured conspecifics. However, little is known about the effects of both cues combined. Therefore, our study evaluated the combined effects of predator odor (PO) and conspecific alarm substance (CAS) on the anti-predatory responses of the pearl cichlid (Geophagus brasiliensis). We set 4 experimental conditions 1) PO + CAS; 2) PO + Distilled Water (DW); 3) DW + CAS; and 4) DW + DW (negative control). Fish exposed to DW + CAS had values of ventilation rate and feeding latency significantly higher than when exposed to the other conditions, which yielded similar responses for both variables. Our data show that the combination of PO + CAS interfered with pearl cichlid behavior and physiology since it abolished CAS defensive responses. These results show that CAS plays an important role on the defensive responses of pearl cichlids, while PO does not, given that fish did not respond to PO by itself. Our findings point to different cue-specific strategies in pearl cichlids when dealing with different chemical cues that indicate predation risk.

Gradients in predation risk in a tropical river system.

The importance of predation risk as a key driver of evolutionary change is exemplified by the Northern Range in Trinidad, where research on guppies living in multiple parallel streams has provided invaluable insights into the process of evolution by natural selection. Although Trinidadian guppies are now a textbook example of evolution in action, studies have generally categorized predation as a dichotomous variable, representing high or low risk. Yet, ecologists appreciate that community structure and the attendant predation risk vary substantially over space and time. Here, we use data from a longitudinal study of fish assemblages at 16 different sites in the Northern Range to quantify temporal and spatial variation in predation risk. Specifically we ask: 1) Is there evidence for a gradient in predation risk? 2) Does the ranking of sites (by risk) change with the definition of the predator community (in terms of species composition and abundance currency), and 3) Are site rankings consistent over time? We find compelling evidence that sites lie along a continuum of risk. However, site rankings along this gradient depend on how predation is quantified in terms of the species considered to be predators and the abundance currency is used. Nonetheless, for a given categorization and currency, rankings are relatively consistent over time. Our study suggests that consideration of predation gradients will lead to a more nuanced understanding of the role of predation risk in behavioral and evolutionary ecology. It also emphasizes the need to justify and report the definition of predation risk being used.

Burrowing owls eavesdrop on southern lapwings' alarm calls to enhance their antipredatory behaviour.

Eavesdropping is a widespread behaviour among animals, providing the receiver with valuable information to assess the habitat, resources or threats. This kind of behaviour has been reported for the burrowing owl (Athene cunicularia), which in its northern range lives in close association with fossorial mammals and eavesdrops on their alarm calls as indicators of risk. In their southernmost range, burrowing owls do not associate with mammals, but they are often found sharing foraging and nesting patches with the southern lapwing (Vanellus chilensis), a noisy, territorial and aggressive plover species. We designed a field experimental study aimed at determining if burrowing owls are able to use lapwing calls as indicator of potential risk. We exposed focal owls to a sequence of sounds including lapwing alarm calls, and biological and non-biological controls, and registered their response as alert or relax behaviours. Linear mixed modeling showed that owls increased their alert behaviour in response to lapwing alarm calls but not in response to control treatments. In addition, owls' response was consistent between habitats (rural and urban) and seasons (breeding and non-breeding). Our results suggest that eavesdropping is a generalized strategy of burrowing owls to acquire environmental information throughout its distribution range.

Relating flight initiation distance in birds to tropical dry forest restoration

Flight initiation distance (FID), defined as the distance at which an individual flees from an approaching predator, might depend on how the individual perceives the risk of being predated. Using a standardized walking approach method on focal bird individuals, we investigated whether different levels of vegetation cover (habitat) influence the perception of predation risk. To do this, we worked in an area of tropical dry forest in central Mexico that is currently part of a restoration ecology experiment. We hypothesized that restoration decreases individuals predation risk perception by increasing the complexity of the vegetation cover. The escape responses of three tropical birds with different diets and foraging strategies were also contrasted. There was no effect of habitat on FID, suggesting that birds in both habitats perceived predation risk in a similar manner. There was, however, a difference in FID among species: the Golden-cheeked Woodpecker tolerated closer human presence before flight than the Inca Dove and Streak-backed Oriole. This difference is likely due to the use of an alternative avoidance strategy of this species, which uses trunks for hiding. To decrease birds perceived predation risk, restoration intervention plans should include a mosaic of larger excluded plots located near relatively well-conserved sites to increase the area covered by vegetation.