Correlation between 146S Antigen Content in Foot-and-Mouth Disease Inactivated Vaccines and Immunogenicity Level and Vaccine Potency Alternative Test Methods.

To investigate the association between 146S antigen contents in FMD inactivated vaccines and levels of antiviral immunity, this study vaccinated 30 kg pigs with three batches of FMD types O and A bivalent inactivated vaccines. Antibody titers and interferon-gamma (IFN-γ) secretion levels were measured on days 7, 14, 21, and 28 after primary immunization and on days 14 and 28 following booster immunization to assess associations between 146S contents and both antibody titers and IFN-γ secretion levels. Furthermore, 30 kg pigs were vaccinated with 46 batches of FMD type O inactivated vaccines and challenged on day 28, after which PD50 values were determined to evaluate the association between 146S content and PD50. The findings suggested that antibody titers and IFN-γ secretion levels at specific time points after immunization were positively associated with 146S contents. Additionally, 146S content showed a positive correlation with PD50, with greater PD50 values recorded for 146S contents ranging from 4.72 to 16.55 µg/dose. This investigation established a significant association between the 146S content in FMD inactivated vaccines and induced immune response against FMDV, thereby emphasizing its critical role in vaccine quality control. The determination of 146S content could serve as a new method for potency testing, offering an alternative to animal challenge tests.

Factors Affecting the Qualification Rate of Hand Disinfection Among Physicians: A Cross-Sectional Survey.

Objective: The objective of this study is to examine the qualification rate of hand disinfection in the surgical department wards and analyze the influencing factors. Methods: A surprise assessment was carried out to evaluate the daily adherence to hand hygiene protocols in the surgical department wards. We aimed to investigate the factors that impact the qualification rate of hand disinfection. Results: In this study, the qualification rate of hand disinfection was determined to be 64.38%. Notably, this rate exhibited significant variations based on gender, age, surgical site, and department category. Specifically, the qualification rate of hand disinfection among female participants stood at 82.35%, surpassing the qualification rate observed among male counterparts of 52.83%. Furthermore, doctors in the age group of 41-50 years demonstrated the highest qualification rate, and the abdominal surgical site exhibited the most noteworthy qualification rate, reaching 79.49%. The outcomes of the multiple logistic regression analysis highlighted the significance of age and gender as influential factors impacting the qualification rate. Specifically, doctors aged 51 years or older exhibited the lowest hand hygiene compliance, whereas female doctors demonstrated a notably higher qualification rate compared to their male counterparts. Conclusion: The disheartening qualification rate of hand disinfection highlights a concerning lack of awareness regarding hand hygiene among surgeons in their professional duties. Consequently, targeted interventions are imperative, focusing on intensified training, educational initiatives, enhanced supervision, and internal performance evaluations for key groups. The findings not only serve as a valuable database but also offer a viable roadmap for similar hospitals to reinforce the management of nosocomial infections.

Microbial monitoring of urban drinking water in Jiangxi Province from 2016 to 2020 / 上海预防医学

ObjectiveTo monitor the microbes in urban drinking water in Jiangxi from 2016 to 2020， to analyze the change in microbial qualification rate， and to provide a scientific basis for government decision-making. MethodsAccording to the Standard Examination Method for Drinking Water （GB/T 5750‒2006） and the Standards for Drinking Water Quality （GB 5749‒2006）， the water samples were collected， tested and evaluated for hygienic safety. The chi-square test was used to compare the qualification rates among different water periods， water source types， water supply modes， water samples， treatment processes， and disinfection methods. ResultsA total of 10 584 water samples were collected and examined from 2016 to 2020，with a qualification rate of 97.72%. The qualified rate of the microbiological index increased gradually over the years. There was no statistically significant difference in the microbiological qualification rate of water samples monitored in different water periods （χ2=0.718，P=0.398）， and the qualification rates were 97.85% and 97.60% in dry and abundant water periods respectively. There was a statistically significant difference in the qualification rates of water samples monitored in different water source types （χ2=79.560，P=0.398）， with groundwater having a higher qualification rate of 98.83% than surface water （97.70%）. The microbiological pass rate of water samples differed among different water supply methods （χ2=201.836，P<0.001）. The microbiological pass rates of municipal centralized water supply， self-built centralized water supply and secondary water supply were 97.51%， 88.94% and 96.56% respectively. The microbiological pass rates of water samples differed among different treatment processes. There were differences in the qualification rates of the water samples between different treatment processes（χ2=855.034，P<0.001）， with 98.22%， 67.19%， 95.60% and 53.13% of the conventionally treated， precipitated filtered， disinfected only and untreated water samples， respectively. The differences in the microbiological qualification rates of the monitored water samples between different disinfection methods were statistically significant （χ2=904.993，P<0.001）. Chlorination disinfection， chlorine dioxide disinfection， other disinfection methods and non-disinfected water samples had the pass rates of 98.35%， 98.19%， 84.38% and 60.16%， respectively. ConclusionThe qualified rate of the microbiological index of drinking water in Jiangxi increases year by year， but there are still some drinking water without appropriate disinfectants and water quality treatment processes. There is still a need to strengthen the purification， disinfection and monitoring of drinking water.

The Impact of Blockchain Application on the Qualification Rate and Circulation Efficiency of Agricultural Products: A Simulation Analysis with Agent-Based Modelling.

In the context of COVID-19, the circulation of agricultural products is increasingly important for the nutrition and health of people. With the changing needs of society and the advancement of technology, the agricultural product circulation system needs to undergo corresponding changes to adapt to the modern fast-paced social system. Blockchain technology couples with the circulation of agricultural products, as its technical features, such as immutability and a distributed ledger database, ensures the speed and stability of the key information circulation process of agricultural products. The research goal of this paper was to clarify the influence of blockchain technology on the qualification rate and circulation efficiency for agricultural products. Based on the main characteristics of blockchain technology and a summary of domestic and foreign theoretical research, this paper simulated the impacts of blockchain technology on the agricultural product circulation system. The results revealed that blockchain technology can improve the qualification rate of agricultural products and thereby ensure their quality and safety. The introduction of blockchain increased the qualification rate by nearly 30%. Moreover, blockchain technology significantly enhanced the efficiency of the agricultural product circulation system, thereby greatly promoting economic benefits. The introduction of blockchain increased circulation efficiency by nearly 15%. Finally, the introduction of blockchain technology can effectively promote the governance level and reduce the supervision costs of the agricultural product circulation system. Through simulation analysis, we found that blockchain technology has a positive impact on both the qualification rate and circulation efficiency for agricultural products. These findings enrich research into the application of blockchain technology in the management and circulation of modern agricultural products.

Analysis of the registered dietitian competence assessment examination during 2017-2021 in China / 中华临床营养杂志

Objective:To analyze the competence assessment examinations of registered dietitians in China during 2017-2021 to inform the training of dietetic professionals.Methods:A systematic review was conducted about the composition and changing trends of examination participants during 2017-2021. Participants were divided into subgroups based on gender, age, education, professional background and affiliations and the qualification rate was compared across different subgroups.Results:The number of examination participants showed an increasing trend overall, with the majority being females and aged 26-35 years. The ratio of participants with bachelor's: master's: doctor's degree was about 15: 9: 1. The proportion of participants majoring in nutrition science decreased from 32.1% to 20.3%, while that of participants majoring in other medical and food sciences increased. Among participants from healthcare institutions, a decreasing proportion of participants were with a major in nutrition science, while this proportion among participants from companies were stable at 38.8% to 50.2%. The average qualification rate was 35.7%, showing no significant differences among different gender and age groups. But the qualification rate varied significantly across subgroups with different education level, professional background, and affiliations ( P < 0.05). Subgroups with more advanced degrees showed higher qualification rate and the best rate was observed in the subgroups with professional background in nutrition science and from nutrition-related institutions. Conclusion:The training of dietetic professionals in China should be improved, lifelong learning should be promoted in nutrition practitioners, and the sustainable development of dietitians should be impelled.

Analysis of monitoring results of disinfection quality in child care institutions in Qingpu District of Shanghai， 2016-2018 / 上海预防医学

Objective:To determine the disinfection effect in child care institutions in Qingpu District of Shanghai， and provide evidence for improving the disinfection quality and preventing and controlling infectious diseases. Methods:According to the monitoring program of disinfection quality in child care institutions in Shanghai， disinfection quality was monitored for 3 years. Results:A total of 979 samples were monitored in the consecutive three years， in which 895 samples were qualified with a total qualification rate of 91.42%. The qualification rate of air samples was 100.00%， while the rate of staff hand samples was the lowest， with an average of 79.60%. During 2016-2018， the difference among the annual qualification rates was statistically significant（χ2=23.809，P<0.05）， whereas it was not significant in the rates between public institutions and private ones （χ2=0.141，P>0.05）. Conclusion:The quality of preventive disinfection in child care institutions in Qingpu District is generally good， but the disinfection of tableware， surface of objects and staff needs to be further improved.

Healthcare failure mode and effect analysis (HFMEA) for improving the qualification rate of disinfection quality monitoring process.

BACKGROUND: This study aimed to assess potential failure mode, implement countermeasures against risks and improve disinfection quality monitoring using healthcare failure mode and effect analysis (HFMEA). METHODS: Between July 2017 and March 2018, a multidisciplinary team was formed to conduct HFMEA and implement improvement interventions. Fourteen monitoring departments and seven monitoring items were involved. The qualification rate of monitoring process was used to evaluate the influence of HFMEA on the standardization monitoring management of disinfection quality. RESULTS: After HFMEA, the qualification rate of overall monitoring process of disinfection quality improved from 16.5% to 78.7% (P < 0.001), and the qualification rates of each monitoring step were all significantly improved. The qualification rate implemented by the clinical laboratory improved from 20.1% to 100.0% (P < 0.001). The qualification rate implemented by thirteen monitoring departments improved from 20.1% to 78.7% (P < 0.001), where seven reached 100%. Out of seven monitored items, three had the qualification rate of 100.0%, while the remaining four items showed significant rising in qualification rates (P < 0.001). CONCLUSION: HFMEA were helpful in improving the qualification rate of disinfection effect monitoring process.

Attribute Discrimination Index-Based Method to Balance Attribute Coverage for Short-Length Cognitive Diagnostic Computerized Adaptive Testing.

We propose a new method that balances attribute coverage for short-length cognitive diagnostic computerized adaptive testing (CD-CAT). The new method uses the attribute discrimination index (ADI-based method) instead of the number of items that measure each attribute [modified global discrimination index (MGDI)-based method] to balance the attribute coverage. Therefore, the information that each attribute provides can be captured. The purpose of the simulation study was to evaluate the performance of the new method, and the results showed the following: (a) Compared with uncontrolled attribute-balance coverage method, the new method produced a higher mastery pattern correct classification rate (PCCR) and attribute correct classification rate (ACCR) with both the posterior-weighted Kullback-Leibler (PWKL) and the modified PWKL (MPWKL) item selection method. (b) Equalization of ACCR (E-ACCR) based on the ADI-based method leads to better results, followed by the MGDI-based method. The uncontrolled method leads to the worst results regardless of item selection methods. (c) Both the ADI-based and MGDI-based methods produced acceptable examinee qualification rates, regardless of item selection methods, although they were relatively low for the uncontrolled condition.

Quality Control System in an Obstetrics and Gynecology Disease Biobank.

AIM: To ensure that sample quality meets the requirements of experimental research, the gynecology and obstetrics biobank of the Nanjing Drum Tower hospital designed different quality control methods for relevant types of samples. A range of quality control procedures has been formulated. METHODS: The sample types were frozen tissue, paraffin-embedded tissue, optimal cutting temperature (OCT)-embedded tissue, plasma, buffy coat, serum, blood clots, and urine. Different categories of samples from a random selection of 1% of cases were analyzed for quality control experiments: (i) frozen tissue, buffy coat, and blood clots: RNA and DNA were extracted and the concentration, purity, and integrity were determined; (ii) paraffin-embedded tissue: morphological observations were made after hematoxylin-eosin staining and immunohistochemical detection of ß-actin or CD10; (iii) OCT-embedded tissue: hematoxylin-eosin staining and immunofluorescence detection of ß-actin; and (iv) frozen tissue samples derived from different organs of 18 fetal autopsy specimens with different cold ischemia times (CITs), 0-12 hours, 12-18 hours, 18-24 hours, and 24-48 hours, were chosen to study RNA quality. There is no universally recognized quality control index for plasma, serum, and urine, so the quality of samples was evaluated from feedback from the research projects in which the samples were used. RESULTS: Currently, there are ∼2000 cases and 360,000 sample vials in the biobank. According to the experiments, (i) the concentration and purity of all nucleic acids of selected samples were qualified; (ii) for frozen tissues with a CIT ≤1 hour, using a qualified standard RNA quality number (RQN) ≥7, the qualification rate was 90%; (iii) frozen tissues with CIT between 1 and 18 hours, using a qualified standard RQN ≥5, the qualification rate was 61.1%; (iv) all of the paraffin-embedded tissues qualified for morphological observation; (v) the qualification rate of OCT-embedded tissue was 89%; and (vi) CIT had a great influence on the integrity of frozen tissue RNA. As the tissue CIT lengthened, the integrity of the RNA decreased. The RNA integrity parameters of different tissue types in the same specimen were significantly different. CONCLUSIONS: A quality control system was constructed in an obstetrics and gynecology disease biobank with various types of diseases and abundant samples. Using specific quality control experiments for different types of samples was a reliable operating strategy that can be beneficial for providing qualified research resources. For birth defect autopsy specimens, the samples used for RNA research should have a CIT of at least <12 hours.

Establishment of Antibiotic Prescription Evaluation Model and the Role of Pharmacist in Prescription Evaluation / 中国药学杂志

OBJECTIVE: To evaluate the effects of prescription evaluation system and the role of pharmacists through the analysis of the results of antibiotic prescription evaluation. METHODS: Random results evaluation analysis based on the results of prescription evaluation in paper during January 2013 to February 2015 and comments of software analysis during March to December 2015. RESULTS: The qualification rates of prescription are respectively 91.6%, 96.5% and 99.1% during 2013-2015 and the consistency of pharmacists and experts are respectively 88.6%, 89.3%, 97.9%, the types of unqualified prescription decrease. CONCLUSION: The evaluation system of antimicrobial agents prescription has accomplished transition from random evaluation of paper prescription evaluation to software ones, which fulfills the mode of prescription evaluation by information techniques.The improving consistency of pharmacists and experts indicates the professional standards of pharmacists.