RESUMO
In radar entomology, one primary challenge is detecting small species (smaller than 5 cm) since these tiny insects reflect radiation that can be poorly observable and, therefore, difficult to interpret. After a literature search on radar entomology, this research found few works where it has been possible to sense insects with dimensions smaller than 5 cm using radars. This paper describes different methodologies to detect Mediterranean fruit flies with 5-6 mm sizes using a pulsed W-band radar and presents the experimental results that validate the procedures. The article's main contribution is the successful detection of Mediterranean fruit flies employing the shadow effect on the backscattered radar signal, achieving an 11% difference in received power when flies are present. So far, according to the information available and the literature search, this work is the first to detect small insects less than 1 cm long using a pulsed radar in W-Band. The results show that the proposed shadow effect is a viable alternative to the current sensors used in smart traps, as it allows not only detection but also counting the number of insects in the trap.
Assuntos
Insetos , Radar , AnimaisRESUMO
(1) Background: Synthetic cannabinoids (SCs) are emerging drugs of abuse sold as 'K2', 'K9' or 'Spice'. Evidence shows that using SCs products leads to greater health risks than cannabis. They have been associated with greater toxicity and higher addiction potential unrelated to the primary psychoactive component of marijuana, Δ9-tetrahydrocannabinol (Δ9-THC). Moreover, early cases of intoxication and death related to SCs highlight the inherent danger that may accompany the use of these substances. However, there is limited knowledge of the toxicology of Spice ingredients. This systematic review intends to analyze the toxicity of SCs compounds in Spice/K2 drugs. (2) Methods: Studies analyzing synthetic cannabinoid toxicity and dependence were included in the present review. We searched the PubMed database of the US National Library of Medicine, Google Scholar, CompTox Chemicals, and Web of Science up to May 2022. (3) Results: Sixty-four articles reporting the effects of synthetic cannabinoids in humans were included in our review. Ten original papers and fifty-four case studies were also included. Fourteen studies reported death associated with synthetic cannabinoid use, with AB-CHMINACA and MDMB-CHMICA being the main reported SCs. Tachycardia and seizures were the most common toxicity symptoms. The prevalence of neuropsychiatric symptoms was higher in third-generation SCs. (4) Conclusion: SCs may exhibit higher toxicity than THC and longer-lasting effects. Their use may be harmful, especially in people with epilepsy and schizophrenia, because of the increased risk of the precipitation of psychiatric and neurologic disorders. Compared to other drugs, SCs have a higher potential to trigger a convulsive crisis, a decline in consciousness, and hemodynamic changes. Therefore, it is crucial to clarify their potential harms and increase the availability of toxicology data in both clinical and research settings.
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The present study aimed to compare the adhesion and proliferation of human periodontal ligament fibroblasts (hPDL) in transverse sections of the teeth sealed with two different obturation techniques, BioRoot RCS/hydraulic obturation (HO) and AH-Plus/continuous-wave condensation (CWC). The techniques were tested using an in vitro model to simulate the interaction between periodontal tissues and the materials. The root canals were instrumented and sterilized. A total of 15 samples were obturated with BioRoot RCS/HO and 15 samples with AH-Plus/CWC. Then, roots were sectioned to obtain obturated teeth slices, and hPDL cells were seeded onto the root slices. The results were obtained at intervals of 4 and 24h for cell adhesion; and at 3,7,14, and 21 days for cell proliferation. Empty cell culture plates were use as controls. The cell adhesion was increased at 4 and 24h for both groups, with an increased response observed in the BioRoot RCS/HO group (p<0.05). The difference in cell proliferation was also found between experimental groups. After 14 days of culture, BioRoot RCS/HO group showed an increase response than control and AH-Plus/CWC groups (p<0.05), and after 21 days both groups behaved better than control group, with an increased response observed in the BioRoot RCS/HO group. This study demonstrated that both root canal sealers allow the attach and growth of periodontal ligament fibroblasts, with an increased biological response in the BioRoot RCS/HO group.
El presente estudio se enfocó en comparar la adhesión y proliferación de fibroblastos de ligamento periodontal humano (hPDL) en secciones transversales de raíces previamente obturadas con dos técnicas de obturación diferentes: obturación hidráulica empleando cono único de gutapercha y BioRoot RCS como sellador (HO), y obturación de condensación de onda continua y AH-Plus como sellador (CWC). Los selladores se usaron en un modelo in vitro que simula la interacción entre los tejidos periodontales y los materiales de obturación. Los conductos radiculares fueron instrumentados, esterilizados y obturados. La muestra se compuso de un total de 15 raíces con la técnica BioRoot RCS/HO y 15 raíces con la técnica AH-Plus/CWC. Las células de hPDL fueron sembradas en condiciones estándar de cultivo sobre las raíces seccionadas. Los resultados fueron obtenidos a intervalos de 4 y 24h para adhesión celular, y a los 3,5,7,14 y 21 días de cultivo para proliferación celular. La adhesión celular a las 4 y 24 horas mostró ser diferente para ambas técnicas en comparación con el grupo control, siendo más importante en el grupo BioRoot RCS/HO. La diferencia en la proliferación entre grupos se observó a los 14 días de cultivo, únicamente para el grupo BioRoot RCS/HO; Sin embargo para el día 21 ambas técnicas mostraron mayor proliferación celular que el grupo control, con mejor respuesta para el grupo BioRoot RCS/HO. Este estudio ha demostrado que ambos selladores de conductos permiten la adhesión y crecimiento de fibroblastos de ligamento periodontal, siendo el grupo BioRoot RCS/HO el que mostró mayor biocompatibilidad.
Assuntos
Humanos , Selantes de Fossas e Fissuras/análise , Teste de Materiais , Ligamento Periodontal , Receptores de Hidrocarboneto ArílicoRESUMO
A common viral replication strategy is characterized by the assembly of intracellular compartments that concentrate factors needed for viral replication and simultaneously conceal the viral genome from host-defense mechanisms. Recently, various membrane-less virus-induced compartments and cellular organelles have been shown to represent biomolecular condensates (BMCs) that assemble through liquid-liquid phase separation (LLPS). In the present work, we analyze biophysical properties of intranuclear replication compartments (RCs) induced during human adenovirus (HAdV) infection. The viral ssDNA-binding protein (DBP) is a major component of RCs that contains intrinsically disordered and low complexity proline-rich regions, features shared with proteins that drive phase transitions. Using fluorescence recovery after photobleaching (FRAP) and time-lapse studies in living HAdV-infected cells, we show that DBP-positive RCs display properties of liquid BMCs, which can fuse and divide, and eventually form an intranuclear mesh with less fluid-like features. Moreover, the transient expression of DBP recapitulates the assembly and liquid-like properties of RCs in HAdV-infected cells. These results are of relevance as they indicate that DBP may be a scaffold protein for the assembly of HAdV-RCs and should contribute to future studies on the role of BMCs in virus-host cell interactions.
Assuntos
Adenoviridae/metabolismo , Condensados Biomoleculares , Proteínas de Ligação a DNA/metabolismo , Compartimentos de Replicação Viral/fisiologia , Replicação Viral/fisiologia , Adenoviridae/genética , Infecções por Adenoviridae , Adenovírus Humanos/metabolismo , Linhagem Celular , Proteínas de Ligação a DNA/química , Interações entre Hospedeiro e Microrganismos , Humanos , Organelas/virologia , Domínios Proteicos , Proteínas Virais/química , Proteínas Virais/genética , Proteínas Virais/metabolismoRESUMO
Sheets of 5754-aluminum alloy processed by a modified repetitive corrugation and straightening (RCS) process were tested in order to measure their formability. For this purpose, forming limit curves were derived. They showed that the material forming capacity decreased after being processed by RCS. However, they kept good formability in the initial stages of the RCS process. The formability study was complemented with microstructural analysis (derivation of texture) and mechanical tests to obtain the strain-rate sensitivity. The texture analysis was done by employing X-ray diffraction, obtaining pole figures, and the orientation distribution function. It was noticed that the initial texture was conserved after successive RCS passes, but the intensity dropped. RCS process did not induce ß-fiber, contrary to common deformation process. The strain-rate sensitivity coefficient was measured through tensile tests at different temperatures and strain rates; the coefficient of the samples processed after one and two passes were still relatively high, indicating the capacity to delay necking, in agreement with the good formability observed in the initial passes of the RCS process.
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Advanced glycation end products (AGEs) represent a set of molecules that contribute directly to the initiation and aggravation of diseases associated with ageing. AGEs are produced by the reaction between reducing sugars (or α-dicarbonyl compounds), proteins, and amino acid residues. Previous in vitro methods using non-enzymatic procedures described in the literature require an incubation period of 1â»3 weeks to generate AGEs. In this study, the reaction time for the formation of AGEs (48 and 3 h) was significantly reduced by adaptation of methods previously described in the literature and coupling them to the free radical generation system termed hypoxanthine/xanthine oxidase assay. The incorporation of this assay into the experimental system accelerated the production of AGEs as a result of the formation of reactive oxygen species (ROS), as shown by increased fluorescence. The capacity of different classes of chemical compounds (aminoguanidine, chlorogenic acid, rutin, and methanol extracts of Hancornia speciosa Gomes) to inhibit protein glycation by acting as scavenging agents of α-dicarbonyl species was evaluated. Aminoguanidine and, especially, rutin identified in the leaf extracts of H. speciosa Gomes showed a high capacity to act as scavengers of reactive carbonyl species RCS-trapping, resulting in the inhibition of AGEs formation.
RESUMO
Abstract The endodontic retreatment is a feasible solution when post-operative apical periodontitis persists or develops. The complete removal of the filling materials is important in order to ensure the unobstructed contact of the intracanal disinfectants with the microbes. As a new generation of bioceramic endodontic sealers has emerged, their removal efficacy from the root canal system during retreatment is a matter of concern among clinicians. The aim of this article is to provide a comprehensive review of the current literature on the retreatability of these novel obturating materials. A significant amount of bioceramic sealer remnants in the root canal walls was observed in all studies. Even though canal cleanliness could not be obtained at an ideal level, the re-establishment of the working length and patency can be considered manageable and comparable to other endodontic sealers.
Resumen El retratamiento endodóntico es una solución factible cuando la periodontitis apical post-operatoria persiste o se desarrolla. La eliminación completa de los materiales obturadores es importante para garantizar el contacto y acción de los desinfectantes e irrigantes endodónticos con los microorganismos persistentes. A medida que ha surgido una nueva generación de selladores endodónticos biocerámicos, su eficacia de eliminación del sistema de conductos radiculares durante el retratamiento es motivo de preocupación entre los profesionales. El objetivo de este artículo es proporcionar una revisión exhaustiva de la literatura actual sobre la influencia o posibles limitantes del uso de este tipo de cementos bioactivos durante el retratamiento endodóntico. Los estudios determinaron una cantidad significativa de restos de selladores biocerámicos en las paredes del conducto radicular. Aunque la limpieza del canal no se pudo obtener a un nivel "ideal", el restablecimiento de la longitud de trabajo y la permeabilidad se puede considerar manejable y comparable a otros selladores endodónticos.
Assuntos
Materiais Restauradores do Canal Radicular/uso terapêutico , Cimentos Dentários/uso terapêutico , Implantação Dentária Endo-Óssea Endodôntica , Cerâmicas Modificadas Organicamente/uso terapêutico , Implantação Dentária Endo-Óssea Endodôntica/tendênciasRESUMO
AIM: To investigate the ability of BioRoot RCS, a tricalcium silicate-based root canal sealer and AH Plus to effectively fill the root canals of contralateral teeth using three evaluation methods, and to investigate also the correlation between the methods. METHODOLOGY: The prepared root canals of ten pairs of contralateral mandibular premolar teeth were filled with gutta-percha and sealer using lateral compaction. The percentage of voids within the root canal was assessed by micro-computed tomography, whilst sealing ability was investigated by fluid transport and leakage of fluorescent microspheres. The interaction of sealer with dentine, and sealer penetration were assessed by confocal microscopy. The void volume, fluid flow, microsphere leakage and sealer interaction with dentine for both materials were compared. Nonparametric (Mann-Whitney) tests were used to compare the % void and fluid transport of the two sealers. Spearman correlation was used to assess the pairwise relationships between the techniques. The level of significance was set to 0.05. RESULTS: BioRoot RCS exhibited significantly more percentage of voids than AH Plus. There was no difference in fluid flow and microsphere penetration. BioRoot RCS exhibited a different pattern of sealer penetration and interaction with the dentine walls compared to AH Plus. For both materials, the pairwise correlations between the three techniques were close to zero, indicating weak relationships. CONCLUSIONS: MicroCT analysis revealed a higher void volume for BioRoot RCS. The other techniques did not show a difference between the sealing ability of the sealers. The correlation between the three ex vivo methods of assessment was weak demonstrating their complementarity rather than their concordance.