Effects of prolonged application of peracetic acid-based disinfectant on recirculating aquaculture systems stocked with Atlantic salmon parr.

The use of recirculating aquaculture systems (RAS) for Atlantic salmon (Salmo salar) production has become increasingly common. RAS water disinfection plays a crucial role on its biosecurity. Peracetic acid (PAA) is a promising disinfectant due to its powerful oxidative properties, broad antimicrobial spectrum, and rapid degradation into no harmful compounds. This study focused on assessing the consequences of prolonged application of a PAA-based disinfectant in a RAS stocked with salmon parr. The experiment included three treatment groups in triplicate: 0 mg/L PAA (control), 0.1 mg/L PAA, and 1 mg/L PAA, using nine-replicated RAS with a total of 360 fish (14.8 ± 2.3 g; N = 40/RAS). The study spanned 28 days, with samples collected on days 0, 14, and 28. The analyzed parameters were water quality, and fish parameters, including external welfare indicators, gill histology, total antioxidant capacity (TAC), reactive oxygen species/reactive nitrogen species (ROC/RNC), oxidative stress biomarkers related to DNA and protein, cellular DNA damage, and global gene expression. While water quality remained relatively stable, there was an increase in bacterial populations in the groups exposed to PAA, particularly 1 mg/L PAA. Fish weight did not differ between the control and PAA-exposed groups. TAC, ROC/RNC, and oxidative stress biomarkers exhibited similar trends. The study identified >400 differentially expressed genes (DEGs) in the skin, gill, and olfactory organ, with many of these DEGs associated with immune responses. Comparing the transcriptomic profiles of the three tissue organs revealed that the olfactory organ was the most reactive to PAA treatment. This study shows that calculated PAA concentrations of 0.1 mg/L and 1 mg/L in the pump-sump, contributed to an increase of bacteria whereas no detectable differences in health and welfare of salmon parr were found. These findings are promising for the implementation of PAA-based disinfectants in RAS stoked with Atlantic salmon parr.

Effects of swimming activity and feed restriction on antioxidant and digestive enzymes in juvenile rainbow trout: Implications for nutritional and exercise strategies in aquaculture.

BACKGROUND: In this study, we investigated the effects of swimming activity and feed restriction on digestion and antioxidant enzyme activities in juvenile rainbow trout (average body weight of 26.54 ± 0.36 g). METHODS: The stomach, liver and kidney tissues were obtained from four distinct groups: the static water group (fish were kept in static water and fed to satiation), the feeding restricted group (fish were kept in static water with a 25% feed restriction), the swimming exercised group (fish were forced to swimming at a flow rate of 1 Body Length per second (BL/s)) and the swimming exercised-feed restricted group (subjected to swimming exercise at a 1 BL/s flow rate along with a 25% feed restriction). We determined the levels of glutathione, lipid peroxidation and the activities of catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase and lactate dehydrogenase, as well as the presence of reactive oxygen species in the tissues obtained from the fish. Additionally, the activities of pepsin, protease, lipase and arginase in these tissues were measured. RESULTS: Swimming activity and feed restriction showed different effects on the enzyme activities of the fish in the experimental groups. CONCLUSION: It can be concluded that proper nutrition and exercise positively influence the antioxidant system and enzyme activities in fish, reducing the formation of free radicals. This situation is likely to contribute to the fish's development.

Transcriptome Analysis of Different Aquaculture Substrates on the Immune Response of Babylonia areolata.

To assess the impact of different substrates in a recirculating water system on the immune response and antioxidant capacity of Babylonia areolata, we conducted a comparative analysis of the transcriptomes and antioxidant performance of the digestive glands in three substrate environments (sand-S group, ceramic granules-C group, and PVC breeding nest-P group). Transcriptome results revealed that the S group and P group exhibited the highest number of differentially expressed genes (DEGs), with a total of 2218 DEGs, including 928 upregulated and 1290 downregulated DEGs. The C group and P group had 1055 DEGs in common, with 316 upregulated and 739 downregulated DEGs. The C group and S group had the fewest DEGs, with 521 in total, including 303 upregulated and 218 downregulated DEGs. GO enrichment analysis showed that in the S vs P group, terms such as catalytic activity, membrane part, and cellular process were enriched with 287, 262, and 180 DEGs, respectively. In the C vs P group, binding, cellular process, and cell part were enriched with 146, 135, and 127 DEGs, respectively. In the C vs S group, catalytic activity, membrane part, and metabolic process were enriched with 90, 83, and 59 DEGs, respectively. Kegg enrichment analysis revealed significant changes in immune-related pathways in the S vs P group, including lysosome, phagosome, and leukocyte transendothelial migration, with 30, 13, and 10 enriched DEGs, respectively. In the C vs P group, phagosome, drug metabolism-other enzymes, and N-Glycan biosynthesis showed significant changes in immune-related pathways, with 9, 6, and 4 enriched DEGs, respectively. In the C vs S group, lysosome, PPAR signaling pathway, and fatty acid degradation exhibited significant changes in immune-related pathways, with 8, 4, and 3 enriched DEGs, respectively. Regarding antioxidant capacity, the S group showed significantly higher total T-AOC than the other experimental groups, while CAT, SOD, POD, and AKP were lower than in the C and P groups. The ACP level in the Sand group was not significantly different from the P group but significantly lower than the C group. In conclusion, substrate environments significantly influence the immune-related genes and key antioxidant enzyme activities in B. areolata.

Low-frequency band noise generated by industrial recirculating aquaculture systems exhibits a greater impact on Micropterus salmoidess.

The effect of underwater noise environment generated by equipment in industrial recirculating aquaculture systems (RAS) on fish is evident. However, different equipment generate noise in various frequency ranges. Understanding the effects of different frequency ranges noise on cultured species is important for optimizing the underwater acoustic environment in RAS. Given this, the effects of underwater noise across various frequency bands in RAS on the growth, physiology, and collective behavior of juvenile largemouth bass (Micropterus salmoides) were comprehensively evaluated here. In this study, three control groups were established: low-frequency noise group (80-1000 Hz, 117 dB re 1µPa RMS), high-frequency noise group (1-19 kHz, 117 dB re 1µPa RMS), and ambient group. During a 30-day experiment, it was found that: 1) industrial RAS noise with different frequency bands all had a certain inhibitory effect on the growth of fish, which the weight gain rate and product of length and depth of caudal peduncle in the ambient group were significantly higher than those of the two noise groups, with the low-frequency noise group showing significantly lower values than the high-frequency noise group; 2) industrial RAS noise had a certain degree of adverse effect on the digestive ability of fish, with the low-frequency noise group being more affected; 3) industrial RAS noise affected the collective feeding behavior of fish, with the collective feeding signal propagation efficiency and feeding intensity of the noise groups being significantly lower than those of the ambient group, and the high-frequency noise group performing better than the low-frequency noise group as a whole therein. From the above, the underwater noise across different frequency bands generated by equipment operation in industrial RAS both had an impact on juvenile largemouth bass, with the low-frequency noise group being more severely affected.

Microplastics in water, feed and tissues of European seabass reared in a recirculation aquaculture system (RAS).

Plastic particles (PLs) are ubiquitous in aquatic ecosystems, and aquaculture production is susceptible to contamination from external or endogenous sources. This study investigated PL presence in water, fish feed and body sites of 55 European seabass produced in a recirculating aquaculture system (RAS). Fish morphometric parameters and health status biomarkers were determined. A total of 372 PLs were recovered from water (37.2 PL/L), 118 PLs from feed (3.9 PL/g), and 422 from seabass (0.7 PL/g fish; all body sites analysed). All 55 specimens had PLs in at least two of the four body sites analysed. Concentrations were higher in the gastrointestinal tract (GIT; 1.0 PL/g) and gills (0.8 PL/g) than in the liver (0.8 PL/g) and muscle (0.4 PL/g). PL concentration in GIT was significantly higher than in muscle. Black, blue, and transparent fibres made of man-made cellulose/rayon and polyethylene terephthalate were the most common PLs in water and seabass, while black fragments of phenoxy resin were the most common in feed. The levels of polymers linked to RAS components (polyethylene, polypropylene, and polyvinyl chloride) were low, suggesting a limited contribution to the overall PL levels found in water and/or fish. The mean PL size recovered from GIT (930 µm) and gills (1047 µm) was significantly larger than those found in the liver (647 µm) and dorsal muscle (425 µm). Considering all body sites, PLs bioconcentrated in seabass (BCFFish >1), but their bioaccumulation did not occur (BAFFish <1). No significant differences were observed in oxidative stress biomarkers between fish with low (<7) and high (≥7) PL numbers. These findings suggest that fish produced in RAS are mainly exposed to MPs through water and feed. Further monitoring under commercial conditions and risk assessment are warranted to identify potential threats to fish and human health and define mitigating measures.

Comparative study on nutritional quality and serum biochemical indices of common carp (Cyprinus carpio) aged 11 to 13 months aged cultured in traditional ponds and land-based container aquaculture systems.

In the study, the physical characteristics, nutritional composition, mineral elements, volatile substances, and serum biochemistry of common carp (Cyprinus carpio) after rearing for two months were compared and analyzed to reveal the differences in muscle nutritional quality and serum biochemical indices between fish raised in traditional ponds (TP) and land-based container recirculating aquaculture systems (C-RAS). One hundred fish were selected from each aquaculture mode for the following experiments. Results show that: in terms of physical properties, C-RAS frozen seepage rate was significantly lower than that in TP (P < 0.05), the chewiness, gumminess, springiness, resilience, adhesiveness, cohesiveness, and shearing of C-RAS group were significantly higher than in TP (P < 0.01). Regarding muscle nutrients, the moisture, the crude lipid contents of C-RAS group were significantly lower than that of the TP group (P < 0.05), and the crude protein content of C-RAS group was extremely significantly higher than that in TP (P < 0.01). The saturated fatty acids (ΣSFA) and polyunsaturated fatty acids (ΣPUFA) in the muscle of C-RAS were significantly higher than that in TP (P < 0.01), and monounsaturated fatty acids (ΣMUFA) of C-RAS were significantly lower than that in TP (P < 0.01). Methionine content in C-RAS was significantly higher than that in TP (P < 0.05). In terms of mineral elements, the contents of K and Se in C-RAS were significantly higher than those in TP, and the content of Zn in C-RAS was significantly lower than that in TP (P < 0.05). In terms of volatile substances, nonanal, octanal, and benzaldehyde in C-RAS were significantly lower than those in TP(P < 0.01), 2,3-diethyl-5-methylpyrazine, ethyl 3-methylpentanoate, butyl formate were significantly higher than those in TP (P < 0.01). In terms of serum biochemistry, the glucose index in C-RAS was extremely significantly lower than that in TP group, total protein, albumin, alkaline phosphatase, and triglyceride were extremely significantly higher than those in TP (P < 0.01). Experiments show that the aquaculture mode has a large impact on the physical properties of the common carp, nutrients, mineral elements, volatile matter, and serum biochemical. In conclusion, the common carp cultured in C-RAS systems had better muscle nutritional quality, which provides a reference for future regulation of fish nutritional quality by C-RAS.

Enhanced aquaculture wastewater treatment in a biofilm reactor filled with sponge/ferrous oxalate/biochar composite (Sponge-C2FeO4@NBC) biocarriers: Performance and mechanism.

Fabricating low-cost and efficient biofilm carriers for moving bed biofilm reactors in wastewater treatment is crucial for achieving environmental sustainability. Herein, a novel sponge biocarrier doped with NaOH-loaded biochar and nano ferrous oxalate (sponge-C2FeO4@NBC) was prepared and evaluated for nitrogenous compounds removal from recirculating aquaculture systems (RAS) wastewater by stepwise increasing ammonium nitrogen (NH4+-N) loading rates. The prepared NBC, sponge-C2FeO4@NBC, and matured biofilms were characterized using SEM, FTIR, BET, and N2 adsorption-desorption techniques. The results reveal that the highest removal rates of NH4+-N reached 99.28 ± 1.3% was yielded by the bioreactor filled with sponge-C2FeO4@NBC, with no obvious nitrite (NO2--N) accumulation in the final phase. The reactor packed with sponge-C2FeO4@NBC biocarrier had the highest relative abundance of functional microorganisms responsible for nitrogen metabolism than in the control reactor, confirmed from 16S rRNA gene sequencing analysis. Our study provides new insights into the newly developed biocarriers for enhancing RAS biofilters treatment performance in keeping water quality within the acceptable level for the rearing of aquatic species.

Positive and negative effects of recirculating aquaculture water advanced oxidation: O3 and O3/UV treatments improved water quality but increased antibiotic resistance genes.

Recirculating aquaculture systems (RASs) can be efficiently used for aquaculture, and oxidation treatment is commonly used to improve water quality. However, the effects of oxidation treatments on aquaculture water safety and fish yield in RASs are poorly understood. In this study, we tested the effects of O3 and O3/UV treatments on aquaculture water quality and safety during culture of crucian carp. O3 and O3/UV treatments reduced the dissolved organic carbon (DOC) concentration by ∼40% and destroyed the refractory organic lignin-like features. There was enrichment of ammonia oxidizing (Nitrospira, Nitrosomonas, and Nitrosospira) and denitrifying (Pelomonas, Methyloversatilis, and Sphingomonas) bacteria, and N-cycling functional genes were enriched by 23% and 48%, respectively, after O3 and O3/UV treatments. Treatment with O3 and O3/UV reduced NH4+-N and NO2--N in RASs. O3/UV treatment increased fish length and weight as well as probiotics in fish intestine. However, high saturated intermediates and tannin-like features induced antibiotic resistance genes (ARGs) in O3 and O3/UV treatments, by 52% and ∼28%, respectively, and also enhanced horizontal transfer of ARGs. Overall, the application of O3/UV achieved better effects. However, understanding the potential biological risks posed by ARGs in RASs and determining the most efficient water treatment strategies to mitigate these risks should be goals of future work.

Occurrence, source tracking and removal of antibiotics in recirculating aquaculture systems (RAS) in southern China.

The recirculating aquaculture system (RAS) has attracted much attention in China as a way to rapidly transform and upgrade aquaculture ponds to realize zero-emissions of pollutants in aquaculture tail water. Tail water purification ponds (TWPPs) play an important role in the treatment of aquaculture wastewater. However, until now, there have been few reports on the occurrence of antibiotics in RAS and the removal of antibiotics from the TWPPs of RAS. Therefore, this study focused on the occurrence of antibiotics in a typical ecological RAS. For comparison, the same measurements were simultaneously carried out in nearby open aquaculture ponds and rivers. The pollution level and spatial distribution of antibiotics in the RAS and the removal of antibiotics in the TWPPs were explored. The results showed that (1) eleven and twelve antibiotics were detected in water and sediment samples in the RAS, respectively, but no antibiotics were found in fish muscles and feed. Erythromycin (ERY), lincomycin (LIN), and ciprofloxacin (CFX) were the three main types of antibiotics found in water and sediment samples. (2) The TWPPs of the RAS can effectively remove antibiotics in aquaculture water. The antibiotic concentration in recirculating aquaculture ponds of the RAS was as high as 180 ng/L. After treatments in the TWPPs, the antibiotic concentration of aquaculture water decreased to 81.6 ng/L (3) The antibiotic concentrations in recirculating aquaculture ponds (25.2-180 ng/L) were lower than those in the nearby open aquaculture ponds (126-267.3 ng/L), and the concentration of antibiotics in the sediments of recirculating aquaculture ponds was up to 22.9 ng/g, while that in TWPPs was as high as 56.1 ng/g. In conclusion, the antibiotic residues in the RAS were low after antibiotics were banned in feed in China, and the removal of antibiotics in the TWPPs was more pronounced. Furthermore, cross-contamination was found between the RAS, surrounding open aquaculture ponds and the river, and the water supply of the RAS was likely to be the main contributor of antibiotics in the aquaculture environments. This study can help the government formulate discharge standards for antibiotics in aquaculture and also provide a reference for the transformation and upgrading of aquaculture ponds to achieve a zero-emission aquaculture mode.

Intensified fish farming. Performance of electrochemical remediation of marine RAS waters.

Aquaculture has been the fastest growing agricultural sector in the past few decades and currently supplies about half of the fish market. A range of environmental and management concerns including limited land and water availability have led to intensifying fish production by recirculating aquaculture systems (RAS). Fish's diet contains 30-60 % protein and about 4-10 % nitrogen (N). As fish assimilate only 20-30 % of the feed to produce body mass, the unassimilated N is released in the form of toxic ammonium that deteriorates water quality and compels its degradation. Widely extended biological nitrification is not efficient in the removal of nitrites nor other chemicals and pharmaceuticals used during fish culture. Electrochemical oxidation, a less developed alternative, reports several advantages such as, i) simultaneous degradation of ammonia­nitrogen (TAN) and water disinfection in the same step with considerable simplification of the whole process, ii) easy adaptability to different production scales and periods of fish growth, and iii) no generation of harmful by-products and no use of chemicals, among others. Besides, in the case of marine aquaculture, the technology benefits from the high conductivity of seawater; thus, electrochemical oxidation is positioned in a very good place to satisfy the water treatment needs of the increasing production rate of marine aquaculture fish. Here, we report the analysis of the performance of a RAS demonstration plant aimed at farming gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) and provided with electrochemical remediation of culture water. The performance of the plant, with 20 m3 of seawater operating at a recirculation rate of 0.9-1.4 h-1, has been analysed in terms of TAN removal, water disinfection, make-up water intake and energy consumption and compared to data of conventional RAS provided with biofilters. The benefits and advantages of the innovative electrochemical remediation of RAS water are highlighted.

Mode of Application of Peracetic Acid-Based Disinfectants has a Minimal Influence on the Antioxidant Defences and Mucosal Structures of Atlantic Salmon (Salmo salar) Parr.

Peracetic acid (PAA) is an oxidative disinfectant with a broad spectrum of antimicrobial activity and low environmental impact. In this study, we investigated the physiological impacts of PAA application in Atlantic salmon (Salmo salar) parr reared in freshwater recirculating aquaculture systems over a 4-week period. PAA at a target concentration of 1 mg/L was administered either in pulse (every 3 days) or continuous. The group that did not receive PAA served as a control. Fish tissue samples were collected for histology, gene expression, and biochemical analyses at day 0 and after 2 and 4 weeks of exposure. The expression of genes encoding for antioxidant defence in the olfactory organs, skin, and gills changed during the trial, but the temporal effects were more pronounced than inter-treatment impacts. The glutathione group of antioxidant genes was more responsive to PAA. In most cases, an upregulation was observed. Significantly lower levels of reactive oxygen species were identified in the plasma and skin mucus of the two PAA-exposed groups at week 4; nonetheless, significantly increased levels of total antioxidant capacity were only observed in the skin mucus of fish from the continuous treatment group. Additional markers of oxidative stress (i.e., 8-oxo-2'-deoxyguanosine and o,o'-dityrosine) were analysed in the skin, gills, liver, and dorsal fins. These markers were unaffected by the two PAA treatments. Sporadic reversible structural alterations were observed in the three mucosal organs; the changes were time-dependent, and the effects of PAA treatment were minimal. The number of mucous cells varied over time but not within treatments except in the skin of the pulse group at week 4 where a reduction was observed. The ratio of acidic and neutral mucous cells in the skin and gills were affected by PAA treatments especially in the pulse group. Overall, this study revealed that Atlantic salmon parr mobilised mucosal and systemic antioxidant defences against the oxidative disinfectant PAA, but it was evident that the mode of application did not impose a strong influence. The minimal effects of PAA application on the indicators of health and welfare underscore the potential use of PAA as a routine disinfectant in recirculating aquaculture systems.

Impact of ozone treatment on dissolved organic matter in land-based recirculating aquaculture systems studied by Fourier transform ion cyclotron resonance mass spectrometry.

In land-based recirculating aquaculture systems (RAS), the accumulation of dissolved organic matter (DOM) can have detrimental effects on water quality impacting the system performance, microbial community, and consequently fish health and welfare. Ozone is used in the RAS water treatment process to improve water quality and remove DOM. However, little is known about the molecular composition of DOM in RAS and its transformation when exposed to ozone. In this study, we performed a detailed molecular characterization of DOM in RAS and explored its transformation induced by ozonation of RAS waters. Ultra-high resolution (UHR) Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) was used to characterize the DOM matrix of RAS waters (pump-sump and tanks) and to evaluate its transformation by ozonation. The analysis of DOM extracted from makeup water and feed samples allowed for the determination of DOM sources in RAS prior to ozonation. The CHO and unsaturated group of compounds were the most abundant class found in water samples. On the contrary, the DOM from feed samples was unique and consisted mainly of CHO, CHON and unsaturated group of compounds. After the ozonation of RAS waters, humic-like and unsaturated compounds [positive oxygen subtracted double bond equivalent per carbon (DBE-O)/C)] were decomposed, particularly the CHO-DOM that contained fewer -CH2- features. Fulvic-like compounds and several hundred saturated compounds [negative (DBE-O)/C)] were formed post ozonation, particularly the CHON and CHONS group of compounds that were associated with fish diets, makeup waters and transformation products from the ozonation of the RAS waters. This study showed that the high accuracy of the ultra-high resolution FTICR MS can be applied to characterize and monitor the changes of DOM at a molecular level in RAS waters. To our knowledge, this is the first study where FTICR MS was incorporated for the characterization of DOM and its sources in RAS.

Anaerobic digestion challenges and resource recovery opportunities from land-based aquaculture waste and seafood processing byproducts: A review.

The unprecedented demand for seafood has resulted in land-based recirculating aquaculture systems (RAS), a highly intensive but sustainable fish farming method. However, intensification also results in concentrated waste streams of fecal matter and uneaten feed. Harvesting and processing vast quantities of fish also leads to the production of byproducts, further creating disposal challenges for fish farms. Recent research indicates that anaerobic digestion (AD), often used for waste treatment in agricultural and wastewater industries, may provide a viable solution. Limited research on AD of freshwater, brackish, and saline wastewater from RAS facilities and co-digestion of seafood byproducts has shown promising results but with considerable operational and process stability issues. This review discusses challenges to AD due to low solid concentrations, salinity, low carbon/nitrogen ratio, and high lipid content in the waste streams. Opportunities for recovering valuable biomolecules and nutrients through microbial treatment, aquaponics, microalgae, and polyhydroxyalkanoate production are also discussed.

Continuous light (relative to a 12:12 photoperiod) has no effect on anxiety-like behaviour, boldness, and locomotion in coho salmon (Oncorhynchus kisutch) post-smolts in recirculating aquaculture systems at a salinity of either 2.5 or 10 ppt.

There is increased interest in rearing salmon in Recirculating Aquaculture Systems (RAS), where environmental conditions can be tightly controlled to optimize growth. Photoperiod and salinity are two important parameters that can be manipulated in RAS. A longer photoperiod permits more time for feeding, while intermediate salinities may reduce the energetic costs of ionoregulation, both of which may enhance growth. However, little is known about how rearing at different photoperiods and salinity affect behaviour, an understudied but important research topic for intensive fish rearing. To address this, we examined the behavioural effects of two salinities and two photoperiod regimes in coho salmon (Oncorhynchus kisutch) post-smolts reared continuously for 120 days in a RAS. Fish were reared on a photoperiod of either 12 h light:12 h dark (12:12), or 24 h light (24:0) at salinities of 2.5 and 10 ppt. To investigate behavioural differences associated with these treatments, we quantified: i) movement in an open-field test, ii) exploratory behaviour/boldness using a novel object approach test, and iii) anxiety-like behaviour with a light/dark test. The 24:0 groups displayed no differences in boldness/anxiety-like behaviour and locomotion relative to the 12:12 groups at their respective salinities. Taken together, fish reared under continuous light (24:0) show negligible behavioural alterations compared to fish reared under normal light dark conditions (12:12).

Impact of underwater noise on the growth, physiology and behavior of Micropterus salmoides in industrial recirculating aquaculture systems.

The operation of the equipment in industrial recirculating aquaculture systems (RAS) affects the underwater soundscape of aquaculture tanks where fishes live. This study evaluated the influence of commercial industrial RAS noise on the growth, physiology, and behavior of juvenile largemouth bass (Micropterus salmoides). In this study, two experimental groups, the RAS noise group (115 dB re 1 µPa RMS) and the ambient group (69 dB re 1 µPa RMS), were studied. The water quality and feeding regime for each group were kept the same during the 60-day experiment. Results showed that there was no significant difference in the average daily feed intake of the fish between the two treatments, while the rate of weight gain of the ambient group (755.27 ± 65.62%) was significantly higher than that of the noise group (337.66 ± 88.01%). In addition, the RAS environmental noise also had an adverse effect on the anti-oxidation and immune systems of the fish based on results of analysis of blood, liver, and intestinal samples. Moreover, environmental noise affected the swimming behavior of the fish school. The mean angle and distance between the focal fish and its nearest neighbor fish in RAS noise group were 33.3° and 92.1 mm, respectively, which were larger than those of the ambient group with 24.4° and 89.5 mm, respectively. From the above results, RAS noise did influence the welfare of largemouth bass, and the soundscape in RAS hence should be managed in real production.

Thermophilic bacteria combined with alkyl polyglucose pretreated mariculture solid wastes using as denitrification carbon source for marine recirculating aquaculture wastewater treatment.

In marine recirculating aquaculture systems (RAS), efficient nitrogen removal is challenging due to the high NO3--N concentration, low organic matters content, and high salinity. In this study, mariculture solid wastes (MSW) acidogenic liquid pretreated by thermophilic bacteria (TB) combined with alkyl polyglucose (APG) was first used as carbon source for denitrification to remove NO3--N. TB + APG pretreatment could accelerate the hydrolysis of MSW, and the highest volatile fatty acids (VFAs) yield (40.3%) was obtained with TB + 0.2 g/g VSS APG pretreatment. MSW acidogenic liquid pretreated by TB + 0.2 g/g VSS APG was a reliable carbon source for denitrification, and the optimum COD/NO3--N ratio (C/N) was 8 with no residue of NOx--N. VFAs were more effectively utilized by denitrifiers than carbohydrate and protein. The high denitrification potential (PDN) and denitrification rate (VDN) indicated the higher denitrification ability at C/N of 8 using MSW acidogenic liquid as carbon source. The outcomes of this work could provide useful information for promoting technological innovation in marine RAS wastewater treatment.

Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems.

Nitrate (NO3-), a potential toxic nitrogenous compound to aquatic animals, is distributed in aquatic ecosystems worldwide. The aim of this study was to investigate the effects of different NO3- levels on growth performance, health status, and endocrine function of juvenile turbot (Scophthalmus maximus) in recirculating aquaculture systems (RAS). Fish were exposed to 0 mg/L (control, CK), 50 mg/L (low nitrate, LN), 200 mg/L (medium nitrate, MN), and 400 mg/L (high nitrate, HN) NO3-N for 60 d in experimental RAS. Cumulative survival (CS) was significantly decreased with increasing NO3- levels in LN, MN, and HN. The lowest CS was 35% in the HN group. Growth parameters, including absolute growth rate, specific growth rate, and feed conversion rate, were significantly different in HN compared with that in the CK. Histological survey of gills and liver revealed dose-dependent histopathological damage induced by NO3- exposure and significant differences in glutamate pyruvate transaminase and glutamate oxalate transaminase in MN and HN compared with that in the CK. The hepatosomatic index in HN was significantly higher than that in the CK. Additionally, NO3- significantly increased bioaccumulation in plasma in LN, MN, and HN compared to that in the CK. Significant decreases in hemoglobin and increases in methemoglobin levels indicated reduced oxygen-carrying capacity in HN. Additionally, qRT-PCR and enzyme-linked immunosorbent assay (ELISA) were developed to investigate key biomarkers involved in the GH/IGF-1, HPT, and HPI axes. Compared with that in the CK, the abundance of GH, GHRb, and IGF-1 was significantly lower in HN, whereas GHRa did not differ between treatments. The plasma T3 level significantly decreased in LN, MN, and HN and T4 significantly decreased in HN. The CRH, ACTH, and plasma cortisol levels were significantly upregulated in HN compared with that in the CK. We conclude that elevated NO3- exposure leads to growth retardation, impaired health status, and endocrine disorders in turbot and the NO3- level for juvenile turbot culture should not exceed 50 mg/L NO3-N in RAS. Our findings indicate that endocrine dysfunction of the GH/IGF-1, HPT, and HPI axes might be responsible for growth inhibition induced by NO3- exposure.

Characterizing changes of dissolved organic matter composition with the use of distinct feeds in recirculating aquaculture systems via high-resolution mass spectrometry.

Recirculating aquaculture systems (RAS) are a new alternative to traditional aquaculture approaches, allowing full control over the fish production conditions, while reducing the water demand. The reduction of water exchange leads to an accumulation of dissolved organic matter (DOM) that can have potential effects on water quality, fish welfare and system performance. Despite the growing awareness of DOM in aquaculture, scarce scientific information exists for understanding the composition and transformation of DOM in RAS. In this study, a non-targeted approach using ultra-performance liquid chromatography coupled to a hybrid quadrupole-time of flight mass spectrometer (UPLC-QTOF-MS) was used to characterize compositional changes of low molecular weight (LMW) DOM in RAS, when operated under two different feed types. A total of 1823 chemicals were identified and the majority of those contained a CHON chemical group in their structure. Changes in the composition of LMW-DOM in RAS waters were observed when the standard feed was switched to RAS feed. The DOM with the use of standard feed, consisted mainly of lignin/CRAM-like, CHO and CHOS chemical groups, while the DOM that used RAS feed, was mainly composed by unsaturated hydrocarbon, CHNO and CHNOS chemical groups. The Bray-Curtis dissimilarity cluster demonstrated differences in the composition of DOM from RAS and was associated to the type of feed used. When the RAS feed was used, the Kendrick mass defect plots of -CH2- homologous units in the pump-sump (after the water treatment) showed a high removal capacity for CHNO, CHNOS and halogenated chemicals with high Kendrick mass defect, KMD > 0.7. To our knowledge, this is the first report of LMW-DOM characterization of RAS by high-resolution mass spectrometry (HRMS).

Performance of novel sponge biocarrier in MBBR treating recirculating aquaculture systems wastewater: Microbial community and kinetic study.

In this study, a novel sponge biocarriers (SB) in moving bed bioreactor (MBBR) treating recirculating aquaculture systems wastewater was evaluated for the first time. Two lab-scale MBBRs were operated simultaneously for 116 days under various hydraulic retention times (HRTs). The reactors R1 and R2 were filled with K5 plastic carriers and SB, respectively. From the results, at an optimum HRT of 6 h, ammonia removal efficiency and nitrification rate were 86.67 ± 2.4% and 1.43 mg/L.h for the R1 and, 91.65 ± 1.3% and 1.52 mg/L.h for the R2, respectively. The microbial community analysis showed that the predominant genera in the nitrifying community were Nitrosomonas (AOB) and Nitrospira (NOB) in co-existence with heterotrophic genera Hyphomicrobium, Mesorhizobium, Zhizhongheella, and Klebsiella spp. Modified Stover-Kincannon model examined the ammonia removal kinetics, and the values of kinetic parameters obtained were Umax: 0.909 and 1.111 g/L.d and KB: 0.929 and, 1.108 g/L.d for the R1 and R2, respectively. The correlation coefficients (R2) of the MBBRs were higher than 0.98, indicating that the model adequately described the experimental data. Overall, MBBR, filled with the proposed novel SB operated at 6 h HRT, can achieve the highest nitrification performance and increase the diversity of the functional microbial communities.

Denitrification performance evaluation and kinetics analysis with mariculture solid wastes (MSW) derived carbon source in marine recirculating aquaculture systems (RAS).

Biological denitrification using mariculture solid wastes (MSW) carbon source is a promising solution for removing nitrate (NO3--N) and disposing MSW in marine recirculating aquaculture systems (RAS). To enhance denitrification performance, heating (HT), rhamnolipid (RL), alkali (AL), thermophilic bacteria (TB) pre-treated MSW acidogenic fermentation effluents were prepared as carbon sources. Profiles of soluble organics in four types of fermentation effluents were first evaluated. The highest volatile fatty acids (VFAs) yield (52.1%) was obtained from TB treated MSW after acidification. RL and TB treated MSW acidogenic fermentation effluents showed high NO3--N removal efficiency (NRE) (around 97%). Acidogenic fermentation effluent from TB treated MSW presented a high biodegradability, with the minimum effluent chemical oxygen demand (COD) amount (35 mg/L). Denitrification kinetics parameters were also analyzed; high fraction (74.5%) of the most readily biodegradable organics (SS) demonstrated that TB treated MSW acidogenic fermentation effluent is a high-quality carbon source for enhancing denitrification.