The environmental consequences of national Audit governance: An analysis based on county-level data in China.

Recent years have witnessed growing public concern over air pollution in China, posing a challenge to the government's environmental management efforts. Empirical evidence indicates that the digital economy contributes to mitigating environmental pollution. Given that national audits are a crucial part of the national oversight system and considering the significant role of digital technology in audit governance, it is relevant to explore how the digital economy can support national audits in enhancing China's environmental quality. This study investigates the environmental impact of national audit governance, utilizing a dataset from 1540 counties in China spanning from 2005 to 2018. The findings reveal that effective national audits contribute to reducing haze pollution (HP) levels, with the digital economy playing a moderating role. The results also demonstrate heterogeneity; national audits are particularly effective in regions characterized by high urbanization rates, severe HP, and stringent environmental regulations. The mechanism analysis suggests that industrial transformation and enhanced government governance are the key mechanisms through which national audits reduce regional HP. Additionally, reforming the audit management system can amplify the effects of national audits on reducing HP.

Downregulated CAV-1 in mouse spinal cord may alleviate bone cancer pain by inhibiting the ERK/CREB pathway.

BACKGROUND: This study aimed to assess the potential function of Caveolin-1 (CAV-1) in mice with bone cancer pain. METHOD: Using a mice bone cancer pain model we explored the contribution of CAV-1 expression to bone cancer pain on the 14th day after surgery, mice in the tumor group were randomized and treated with increasing doses of the CAV-1 inhibitor, methyl-beta-cyclodextrin. Pain was assessed by monitoring the number of spontaneous flinches (NSF) and paw withdrawal mechanical threshold (PMWT)mechanical withdrawal threshold (MWT). The localization and expression of CAV-1 in mouse neurons was also determined. Additionally, the protein levels of CAV-1, extracellular signal regulated kinase (ERK) 1/2, cAMP response element-binding protein (CREB) were monitored in mouse spinal cord tissues by western blotting. RESULTS: CAV-1 was remarkably upregulated in the spinal cord of the tumor group on the 4th day after surgery, then downregulated on day 10, and upregulated again at day 14. Such CAV-1 levels were maintained until day 28. In the tumor group, the expression of p-ERK1/2 and p-CERB were upregulated at day 14 after surgery. Intrathecal injection of methyl-beta-cyclodextrin (MCD) downregulated p-ERK1/2 and p-CERB expression which correlated with alleviation of pain. CONCLUSION: Inhibition of CAV-1 in the spinal cord alleviates bone cancer pain in mice which correlates with inhibition of the ERK/CREB pathway.

Impact of logistics agglomeration on environmental quality in China: aggravating pollution effect or emission reduction effect?

Whether logistics agglomeration and pollution reduction can form a perfect combination in space is one of the important breakthroughs for the successful green transformation of the logistics industry in the future. This paper attempts to clarify the nonlinear change law of logistics agglomeration evolution on environmental quality in China and further clarify the interpretation of informatization and legalization factors on macro-regulatory functions. Based on the panel data of 31 provincial-level administrative regions in China from 2010 to 2020, the empirical verification is carried out by means of two-way fixed effects, quantile regression, instrumental variables, and spatial econometrics. The research finds that (1) there is an inverted U-shaped change trend between logistics agglomeration and environmental quality and the low-level logistics agglomeration period has a blocking effect on pollution emission reduction. However, from the starting point of the sample interval, representative regions such as Beijing and Shanghai gradually break through the bottleneck of logistics agglomeration in the form of a pyramid, which has an incentive effect on pollution emission reduction. The above conclusions are supported by robustness tests in six ways, including eliminating extreme data disturbance, eliminating the impact of the COVID-19, controlling the impact of other factors, pollutant sensitivity testing, avoiding the interference of pollution prevention and control, and correcting the endogenous bias of the model. (2) Further analysis of superimposed spatial effects and regulatory effects shows that regional spillover pollution has spatial dependence in both geographical and economic distribution. The effect of logistics agglomeration also produces an inverted U-shaped spatial spillover effect on the environmental quality of adjacent areas. Both informatization and legalization factors help alleviate the pollution increasing effect in the low-level logistics agglomeration stage. Under the regulatory effect of informatization, the arrival of the critical point for logistics agglomeration and emission reduction will be relatively delayed. Under the regulatory effect of legalization, the arrival of the critical point for logistics agglomeration and emission reduction will be relatively accelerated. (3) There is significant regional heterogeneity in the environmental effects of logistics agglomeration. Each region needs to "suit the remedy to the case" in combination with its own situation to break through the threshold of pollution reduction as soon as possible.

The Regulation Effect of Team Leadership and Cohesion on Athletes' Sports Grade / El efecto de regulación del liderazgo y la cohesión del equipo en el grado deportivo de los atletas

Team cohesion is a crucial element in bringing the group together. Team cohesion is the engine that drives the progression of a sports team, and it plays a crucial part in boosting the team's confidence and overall performance. This study aims to investigate the connection between athlete leadership and team cohesion and the potential mediating and regulating effects between emotional infection and sports grade. The Athlete Leadership Questionnaire, Group Environment Questionnaire, and Emotional Infection Scale were administered to 316 athletes, and the data were analyzed using SPSS. The Bootstrap approach was used to determine whether emotional infection significantly mediated the link between athlete conduct and team cohesion. In the main effect, athlete leadership can positively and significantly predict team cohesion (β=0.531, p<0.01), and the interaction item between athlete's leadership and sports level also reached a significant level (β=0.355, p<0.01). Age and sex did not pass the F test (F=1.148, p>0.05), indicating that they will not affect group cohesion. This study found that team cohesiveness and external leaders had the most impact on social cohesion, but social leaders had the greatest impact on task cohesion. In addition, the study found that emotional infection serves as a partial mediator between the leadership of athletes and their numerous aspects and team cohesion.(AU)

Comparative Genomic and Transcriptomic Analyses Reveal the Impacts of Genetic Admixture in Kazaks, Uyghurs, and Huis.

Population admixture results in the combinations of genetic components derived from distinct ancestral populations, which may impact diversity at the genetic, transcriptomic, and phenotypic levels, as well as postadmixture adaptive evolution. Here, we systematically investigated the genomic and transcriptomic diversity in Kazaks, Uyghurs, and Huis-three admixed populations of various Eurasian ancestries living in Xinjiang, China. All three populations showed elevated genetic diversity and closer genetic distance compared with the reference populations across the Eurasian continent. However, we also observed differentiated genomic diversity and inferred different demographic histories among the three populations. Varying ancestry proportions observed in both the global and local aspects corresponded to the population-differentiated genomic diversity, with the most representative signals observed in the genes EDAR, SULT1C4, and SLC24A5. The varying local ancestry partly resulted from the postadmixture local adaptation, with the most significant signals observed in immunity- and metabolism-related pathways. Admixture-shaped genomic diversity further influenced the transcriptomic diversity in the admixed populations; in particular, population-specific regulatory effects were associated with immunity- and metabolism-involved genes such as MTHFR, FCER1G, SDHC, and BDH2. Furthermore, differentially expressed genes between the populations were identified, many of which could be explained by the population-specific regulatory properties, including genes related to health concerns (e.g., AHI1 between Kazak and Uyghurs [P < 6.92 × 10-5] and CTRC between Huis and Uyghurs [P < 2.32 × 10-4]). Our results demonstrate genetic admixture as a driving force in shaping the genomic and transcriptomic diversity of human populations.

Employee equity incentive, executive psychological capital, and enterprise innovation.

The input and deployment of human resources, such as senior executives and the core employees involved in different innovation processes, is key to developing enterprise innovation activities. Under the same framework, it is crucial to explore how employee equity incentive and senior executives' psychological capital affect enterprise innovation. This paper's research sample comprises listed companies that implemented equity incentives in the A-share market from 2010 to 2021, examining the relationship between executive psychological capital, and enterprise innovation. This study found that: (1) Employee equity incentive and executives' psychological capital can significantly improve the quality of innovation output while promoting enterprises to increase the quantity of innovation outputs; (2) Executive psychological capital contributes to the intermediary effect between employee equity incentive and enterprise innovation; (3) R&D investment has a positive moderating effect on employee equity incentive, senior executives' psychological capital and enterprise innovation; (4) The innovation incentive effect brought by the implementation of stock options by enterprises is more significant, which also makes the psychological capital of executives maintain a positive role in enterprise innovation; (5) In addition, the positive effects of employee equity incentive and executive psychological capital on enterprise innovation are affected by different property rights. The positive effects of employee equity incentive and executive psychological capital on enterprise innovation in state-owned enterprises are not high.

Herders' adaptation strategies and animal husbandry development under climate change: A panel data analysis.

The frequent occurrence of extreme climate events has become an indisputable fact. However, the role of adaptation to extreme climate change in the development of livestock husbandry is still insufficiently understood. This study empirically analyzed the impact of herders' adaptation strategies to extreme drought on livestock husbandry development and aimed to explore the optimal grassland management path under continuous climate change. A panel dataset of surveyed herders from the Xilingol League, a traditional pastoral area in China, was used. The results indicated that the average frequency of extreme drought in the Xilingol League from 1980 to 2020 was 4.94 months/year, and the occurrence of extreme drought showed a slightly upward trend. The average technical efficiency of livestock husbandry was 0.721, which can still be improved. Hay purchases can effectively promote livestock technical efficiency (p<0.01) and is the main adaptation strategy of herders to extreme drought. Further analysis showed that non-farming and pastoral employment has a positive regulatory effect in the impact of purchased hay on livestock technical efficiency. The results of this study deepen the understanding of effective adaptation to extreme weather events in pastoral areas due to climate change and provide useful information to policymakers engaged in grassland management.

DeepsmirUD: Prediction of Regulatory Effects on microRNA Expression Mediated by Small Molecules Using Deep Learning.

Aberrant miRNA expression has been associated with a large number of human diseases. Therefore, targeting miRNAs to regulate their expression levels has become an important therapy against diseases that stem from the dysfunction of pathways regulated by miRNAs. In recent years, small molecules have demonstrated enormous potential as drugs to regulate miRNA expression (i.e., SM-miR). A clear understanding of the mechanism of action of small molecules on the upregulation and downregulation of miRNA expression allows precise diagnosis and treatment of oncogenic pathways. However, outside of a slow and costly process of experimental determination, computational strategies to assist this on an ad hoc basis have yet to be formulated. In this work, we developed, to the best of our knowledge, the first cross-platform prediction tool, DeepsmirUD, to infer small-molecule-mediated regulatory effects on miRNA expression (i.e., upregulation or downregulation). This method is powered by 12 cutting-edge deep-learning frameworks and achieved AUC values of 0.843/0.984 and AUCPR values of 0.866/0.992 on two independent test datasets. With a complementarily constructed network inference approach based on similarity, we report a significantly improved accuracy of 0.813 in determining the regulatory effects of nearly 650 associated SM-miR relations, each formed with either novel small molecule or novel miRNA. By further integrating miRNA-cancer relationships, we established a database of potential pharmaceutical drugs from 1343 small molecules for 107 cancer diseases to understand the drug mechanisms of action and offer novel insight into drug repositioning. Furthermore, we have employed DeepsmirUD to predict the regulatory effects of a large number of high-confidence associated SM-miR relations. Taken together, our method shows promise to accelerate the development of potential miRNA targets and small molecule drugs.

Research progress of S1P signaling pathway in eye diseases / 中华实验眼科杂志

Sphingolipid metabolism is widely involved in the functional regulation of different cells, and also plays an important role in ocular tissues.Sphingosine 1-phosphate (S1P) is the end product of sphingolipid metabolism and has been shown to play an important role in the onset and development of eye diseases.S1P signaling pathway is widely expressed in various ocular cells and is involved in the regulation of cell proliferation, differentiation, migration and apoptosis.S1P activates a variety of signaling pathways by binding to corresponding receptors and thus plays a wide range of physiological and pathological effects in the eye.Recent studies have found that the S1P signaling pathway can not only mediate the normal development of blood vessels and nerves in the eye, maintain the normal structure of the ocular tissues, and participate in the metabolism of lipids in the eye, but also has a close relationship with immune-related inflammatory response, pathological fibrosis, destruction of cell functional barrier and other related pathological changes.This paper mainly reviewed the basic overview of the S1P signaling pathway, its physiological role in the eye, and its role in the pathological changes of anterior and posterior segment diseases, so as to provide new directions and targets for the treatment of eye diseases.

Apigenin-Mn(II) loaded hyaluronic acid nanoparticles for ulcerative colitis therapy in mice.

Ulcerative colitis (UC) is a chronic idiopathic inflammatory bowel disease characterized by rapid progression and frequent comorbidities that make its treatment challenging. Nanomaterial-based strategies have been extensively studied to target the GI mucosal immune system in recent years. Herein, we propose a novel apigenin-Mn(II) loaded sodium hyaluronate nanoparticles where apigenin (API) was incorporated in the Mn2+ ramework, coated with hyaluronic acid. The apigenin-Mn(II) loaded sodium hyaluronate nanoparticles (API-Mn(II)@HA NPs) exhibited a diameter of 200 nm and were effective against UC. The preparation of the API-Mn(II) complex was relatively simple, and the mechanism underlying its therapeutic effect on UC induced by sodium dextran sulfate (DSS) was studied in detail. We found that API-Mn(II)@HA nanoparticles could effectively repair the intestinal barrier and significantly improve the damaged colon tissue by mediating inflammatory factors. This study provides novel insights on a new kind of active targeted nanoparticle for improving the efficacy of drugs for UC treatment.

Immunomodulatory Effects of Bifidobacterium spp. and Use of Bifidobacterium breve and Bifidobacterium longum on Acute Diarrhea in Children.

The intake of probiotic lactic acid bacteria not only promotes digestion through the microbiome regulated host intestinal metabolism but also improves diseases such as irritable bowel syndrome and inflammatory bowel disease, and suppresses pathogenic harmful bacteria. This investigation aimed to evaluate the immunomodulatory effects in intestinal epithelial cells and to study the clinical efficacy of the selected the Bifidobacterium breve and Bifidobacterium longum groups. The physiological and biochemical properties were characterized, and immunomodulatory activity was measured against pathogenic bacteria. In order to find out the mechanism of inflammatory action of the eight viable and sonicated Bifidobacterium spp., we tried to confirm the changes in the pro-inflammatory cytokines (TNF-α, interleukin (IL)-6, IL-12) and anti-inflammatory cytokine (IL-10), and chemokines, (monocyte chemoattractant protein-1, IL-8) and inflammatory enzymatic mediator (nitric oxide) against Enterococcus faecalis ATCC 29212 infection in Caco-2 cells and RAW 264.7 cells. The clinical efficacy of the selected B. breve and B. longum group was studied as a probiotic adjuvant for acute diarrhea in children by oral administration. The results showed significant immunomodulatory effects on the expression levels of TNF-α, IL-6, IL-12, MCP-1, IL-8 and NO, in sonicated Bifidobacterium extracts and viable bifidobacteria. Moreover, each of the Bifidobacterium strains was found to react more specifically to different cytokines. However, treatment with sonicated Bifidobacterium extracts showed a more significant effect compared to treatment with the viable bacteria. We suggest that probiotics functions should be subdivided according to individual characteristics, and that personalized probiotics should be designed to address individual applications.

Regulatory Effect of Irresistin-16 on Competitive Dual-Species Biofilms Composed of Streptococcus mutans and Streptococcus sanguinis.

Based on the ecological plaque hypothesis, suppressing opportunistic pathogens within biofilms, rather than killing microbes indiscriminately, could be a biofilm control strategy for managing dental caries. The present study aimed to evaluate the effects of irresistin-16 (IRS-16) on competitive dual-species biofilms, which consisted of the conditional cariogenic agent Streptococcus mutans (S. mutans) and oral commensal bacteria Streptococcus sanguinis (S. sanguinis). Bacterial growth and biofilm formation were monitored using growth curve and crystal violet staining, respectively. The microbial proportion was determined using fluorescence in situ hybridization. A 2, 5-diphenyltetrazolium bromide assay was used to measure the metabolic activity of biofilms. Bacterial/extracellular polysaccharide (EPS) dyeing, together with water-insoluble EPS measurements, were used to estimate EPS synthesis. A lactic acid assay was performed to detect lactic acid generation in biofilms. The cytotoxicity of IRS-16 was evaluated in mouse fibroblast L929 cells using a live/dead cell viability assay and cell counting kit-8 assay. Our results showed that IRS-16 exhibited selective anti-biofilm activity, leading to a remarkable survival disadvantage of S. mutans within competitive dual-species biofilms. In addition, the metabolic activity, EPS synthesis, and acid generation of dual-species biofilms were significantly reduced by IRS-16. Moreover, IRS-16 showed minimal cytotoxicity against mouse fibroblast L929 cells. In conclusion, IRS-16 exhibited remarkable regulatory effects on dual-species biofilms composed of S. mutans and S. sanguinis with low cytotoxicity, suggesting that it may have potential for use in caries management through ecological biofilm control.

Foreign direct investment, environmental regulation, and haze pollution: empirical evidence from China.

As China's economy continues to grow, more and more attention is being paid to environmental pollution issues, including haze pollution, which is the most prominent of the air problems. In order to alleviate the haze pollution problem while ensuring the normal operation of the economy, the government has adopted an environmental regulation policy along with the introduction of foreign direct investment. This study uses a fixed effects model and two-stage least squares to examine the correlation of foreign direct investment and environmental regulation to the haze pollution level by using the Chinese provincial panel data from 2008 to 2019. We found a positive interaction between the foreign direct investment and environmental regulation, where a high-quality investment promotes the haze reduction, and the environmental regulation can enhance this effect. The results approve the possibilities that China can bring the merit investment and reduce its air pollution simultaneously, but how to balance the interaction between environmental regulation and the investment needs to draw attention.

[Research progress of circadian rhythm].

Circadian rhythm disorder is a common society issue caused by jet lag,shift work,sleep disruption and changes in food consumption. Light is the major factor affecting the circadian rhythm system. Disruption of the circadian rhythm system can cause damage to the body,leading to some diseases. Maintaining a normal circadian system is of great importance for good health. Ideal therapeutic effect can not only alleviate symptoms of the diseases,but also recovery the disturbed circadian rhythm to normal. The paper summarizes the modeling methods of animal circadian rhythm disorder,diseases of circadian rhythm abnormality,regulation of circadian clock genes and medicine which are related to circadian rhythm to diseases of circadian rhythm disorder.

[Research Progress of FAM60A in the Regulation of Cellular Function].

FAM60A,a cell cycle protein,is a subunit of the SIN3 transcription regulator family member A/histone deacetylase(SIN3-HDAC)complex and plays an important role in cell cycle regulation,cell morphology change,cell proliferation,differentiation and migration,early embryogenesis and so on.Studies in recent years have shown that FAM60A plays a role in the occurrence and development of tumors including human osteosarcoma,esophageal cancer,gastric cancer,lung cancer and liver cancer,providing a new research direction for tumor diagnosis and treatment.Based on the research results in recent years at home and abroad,this paper discussed the effects of FAM60A on cellular functions.

Regulatory effect of m6 A modification on different viruses.

N6 -methyladenosine (m6 A) modification is the most common and reversible posttranscriptional modification of RNA in eukaryotes, which is mainly regulated by methyltransferase, demethylase, and specific binding protein. The replication of the virus and host immune response to the virus are affected by m6 A modification. In different kinds of viruses, m6 A modification has two completely opposite regulatory functions. This paper reviews the regulatory effects of m6 A modification on different viruses and provides a reference for studying the regulatory effects of RNA epitranscriptomic modification.

[Review on characteristics of acupuncture-activated network regulatory effect based on brain connectomics].

The network analysis method based on brain connectomics is an important entry point to explore the working mechanism of brain and is also the current trend of researches on acupuncture stimulation-induced changes of neuroimages. We, in the present review, summarized the common network analysis methods for exploring the underlying mechanisms of brain network-mediated regulatory effects of acupuncture interventions. Moreover, combining the current research development and our team's previous research findings, we extracted the characteristics of targeting, conditionity and dynamic of regulatory effects of acupuncture-activated brain network, and put forward our prospects about the future research from the aspects of new scanning modes (for instance, multimodal data acquisition of magnetic resonance, electroencephalogram, near infrared spectrum, etc.), network analysis (such as Granger causality analysis, complex network measures, whole-brain connectivity dynamics tracking, high-order resting-state functional connectivity analysis, etc.) and experimental research paradigms (for example, introduce of transcranial magnetic stimulation induced transient changes of brain functional activity, machine learning approach, etc.).

Lymphocyte modulation by tofacitinib in patients with rheumatoid arthritis.

Tofacitinib is an oral small molecule targeting the intracellular Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathways approved for the treatment of active rheumatoid arthritis (RA). We investigated the effects of tofacitinib on the response of RA lymphocytes to B and T cell collagen epitopes in their native and post-translationally modified forms. In particular, peripheral blood mononuclear cells (PBMCs) from patients with RA and healthy subjects were cultured with type II collagen peptides (T261-273, B359-369, carT261-273, citB359-369) or with phorbol myristate acetate (PMA)/ionomycin/CD40L in the presence or absence of 100 nM tofacitinib for 20 h and analyzed by fluorescence activated cell sorter (FACS). Cultures without brefeldin A were used for cytokine supernatant enzyme-linked immunosorbent assay (ELISA) analysis. Tofacitinib down-regulated inflammatory cytokines by stimulated B [interleukin (IL)-6 and tumor necrosis factor (TNF)-α] and T [interferon (IFN)-γ, IL-17 or TNF-α] cells in the short term, while a significant reduction of IL-17 and IL-6 levels in peripheral blood mononuclear cell (PBMC) supernatant was also observed. IL-10 was significantly reduced in collagen-stimulated B cells from patients with RA and increased in controls, thus mirroring an altered response to collagen self-epitopes in RA. Tofacitinib partially prevented the IL-10 down-modulation in RA B cells stimulated with collagen epitopes. In conclusion, the use of tofacitinib exerts a rapid regulatory effect on B cells from patients with RA following stimulation with collagen epitopes while not reducing inflammatory cytokine production by lymphocytes.

Critical review on soil phosphorus migration and transformation under freezing-thawing cycles and typical regulatory measurements.

Freezing-thawing period plays an important role in the soil nutrient cycling. The frequency of freezing-thawing cycles (FTCs) can directly affect the supply of effective soil nutrients, further influences the growth and development of crops. Phosphorus is one of the essential nutrients for crop growth, and almost no compounds in gas form in nature, which is non-renewable resources. In modern agricultural production, phosphorus required by plants is mainly from the soil, but the utilization rate of phosphorus fertilizer in soil is generally only 10%-25%. Therefore, it is of great significance to study phosphorus migration and transformation behavior of soil in the non-growth period and related interfacial processes for improving the utilization efficiency of phosphorus fertilizer, increasing crop yield, reducing excessive application of phosphorus fertilizer, and subsiding environmental pollution. This paper systematically concludes key interfacial process of soil phosphorus in freezing-thawing soil system and relative mechanisms describing migration and transformation behavior of soil phosphorus. Besides, it summarizes the mediating effects of widely used soil conditioner on phosphorus cycling. The results show that freezing- thawing will destroy the structure of the soil, causing phosphorus to migrate along with runoff, soil water and heat movement. It also affects the types of microorganisms, the activity of microbial communities and the oxidation-reduction reaction of related minerals, making the phosphorus in soil from an unstable form to an active form. Biochar and humic substances can improve the physical and chemical properties of the soil, and have favorable effects on soil during freezing-thawing period. This review has important significance for the rational utilization of existing phosphorus resources, the maintenance of soil phosphorus cycle balance and the sustainable development of agriculture, meanwhile, has guiding significance for the reasonable utilization of agricultural wastes.

Research progress of circadian rhythm / 中国中药杂志

Circadian rhythm disorder is a common society issue caused by jet lag,shift work,sleep disruption and changes in food consumption. Light is the major factor affecting the circadian rhythm system. Disruption of the circadian rhythm system can cause damage to the body,leading to some diseases. Maintaining a normal circadian system is of great importance for good health. Ideal therapeutic effect can not only alleviate symptoms of the diseases,but also recovery the disturbed circadian rhythm to normal. The paper summarizes the modeling methods of animal circadian rhythm disorder,diseases of circadian rhythm abnormality,regulation of circadian clock genes and medicine which are related to circadian rhythm to diseases of circadian rhythm disorder.