RESUMO
Objective To explore the reverse effect and mechanism of progesterone(Prog) on a human tongue cancer drug-resistant cell line Tca8113/BLM in vitro. Methods The reverse effect of Prog on Tca8113/BLM was determined by MTT. The effects of Prog on the intracellular ADM congregation, cell cycle distribution and the expression of P-gp in the cell membrane were detected by flow cytometry (FCM). Results The value of IC50 of Tca8113 and Tca8113/BLM cells without Prog was 9 56 and 120 1,respectively. After treated with Prog(2umol/L),The value of IC50 of Tca8113 and Tca8113/BLM cells was 9 56 and 12 1,respectively. The potent fold of BLM in Tca8113/BLM cells was 9 92, the concentration of intracellular ADM in Tca8113/BLM cells significantly increased(P
