Prediction models of carcass characteristics from non­castrated Nellore cattle finished in the feedlot system under tropical conditions.

Our objective was to use measures of intake and productive performance to adjust prediction models for the carcass traits of non-castrated Nellore cattle finished in a feedlot. Individual data from 168 non-castrated male Nellore steers finished in feedlot between the years 2016-2021 were used. Descriptive statistical analyzes and Pearson correlation coefficients were performed. The outliers were tested by evaluating the studentized residuals in relation to the values predicted by the equations. Residues that were outside the range of -2.5 to 2.5 were removed. The goodness of fit of the developed equations was evaluated by the coefficients of determination (R2) and root mean square error (RMSE). Models for carcass yield, subcutaneous fat thickness, ribeye area, and shear force were adjusted. Means of 53.5% carcass yield, 4.8 mm subcutaneous fat thickness, 73 cm2 loin eye area, and 8.1 kg shear force were observed. The observed average intakes were 9.9 kg/day of dry matter, 3.3 kg/day of neutral detergent fiber content, 1.5 kg/day of crude protein, and 7.1 kg/day of total digestible nutrients. The average confinement time was 113 days, the average total weight gain was 152.2 kg and the average daily gain was 1.35 kg/day. Intake measures significantly correlated with shear force and subcutaneous fat thickness and ribeye area. Carcass yield was significantly correlated with total weight gain, feedlot time, and hot carcass weight. Measures of nutrient intake, performance, and confinement time can be used as predictors of carcass yield, ribeye area, fat thickness, and shear force of non-castrated Nellore cattle finished in a feedlot. The prediction equations for ribeye area, carcass yield, subcutaneous fat thickness, and shear force showed sufficient precision and accuracy for non-castrated Nellore cattle finished in confinement systems under tropical conditions. All equations can be used with caution to estimate carcass traits of cattle finished in a feedlot using measures of intake and productive performance.

Carcass Traits of Growing Meat Goats Fed Different Levels of Hempseed Meal.

Hempseed meal (HSM) is the byproduct of hemp seeds and is rich in crude protein and fiber, making it an ideal candidate as a feedstuff for ruminants. The objective of the present study is to evaluate the effects of feeding different levels of HSM on the carcass traits of crossbred Boer goats. Forty castrated goat kids (approximately six months, 25.63 ± 0.33 kg) were assigned to one of four treatments (n = 10) in a completely randomized design. Goats were fed pelleted diets (50% forage and 50% concentrate) with additional supplementation of HSM: control with 0%, 10%, 20%, and 30% of the total diets. Goats were harvested and processed after a 60-day feeding trial. There were no significant differences (p > 0.05) in the mean values of dressing percentages, carcass weights, body wall thickness, and ribeye area among treatments. Marbling scores and percentages of moisture, fats, proteins, and collagen in the muscles showed no significant differences (p > 0.05) among the treatments. Results suggest that including up to 30% of HSM in the diet of growing meat goats does not affect their carcass traits.

Palatability assessments of beef strip loin steaks portioned by weight or by thickness sourced from various carcass weight/ribeye area size combinations.

Beef carcasses (n = 90; U.S. Choice) met a 3 ribeye area (REA - Small, Medium, Large) × 3 carcass weight (CW - Light, Intermediate, Heavy) scheme to assess palatability on steaks cut by portion thickness (PT- 3.18 cm) and weight (PW -340 g). Significant interactions revealed trends for steaks from the Small REA, regardless of CW, to have among the lowest shear-force values. For PT steaks, significant interaction for overall liking revealed no differences for Small and Medium REA across all CW categories, but steaks from Large REA from Light CW differed (P < 0.05) from the other two CW categories. For PT steaks, overall liking and tenderness liking scores were higher (P < 0.05) for Small REA compared to other categories, whereas CW did not influence any palatability trait. REA and CW do impact beef steak palatability, though steaks from all combinations were "very tender" and highly acceptable from a palatability standpoint.

Gene co-expression networks associated with carcass traits reveal new pathways for muscle and fat deposition in Nelore cattle.

BACKGROUND: Positively correlated with carcass weight and animal growth, the ribeye area (REA) and the backfat thickness (BFT) are economic important carcass traits, which impact directly on producer's payment. The selection of these traits has not been satisfactory since they are expressed later in the animal's life and multigene regulated. So, next-generation technologies have been applied in this area to improve animal's selection and better understand the molecular mechanisms involved in the development of these traits. Correlation network analysis, performed by tools like WGCNA (Weighted Correlation Network Analysis), has been used to explore gene-gene interactions and gene-phenotype correlations. Thus, this study aimed to identify putative candidate genes and metabolic pathways that regulate REA and BFT by constructing a gene co-expression network using WGCNA and RNA sequencing data, to better understand genetic and molecular variations behind these complex traits in Nelore cattle. RESULTS: The gene co-expression network analysis, using WGCNA, were built using RNA-sequencing data normalized by transcript per million (TPM) from 43 Nelore steers. Forty-six gene clusters were constructed, between them, three were positively correlated (p-value< 0.1) to the BFT (Green Yellow, Ivory, and Light Yellow modules) and, one cluster was negatively correlated (p-value< 0.1) with REA (Salmon module). The enrichment analysis performed by DAVID and WebGestalt (FDR 5%) identified eight Gene Ontology (GO) terms and three KEGG pathways in the Green Yellow module, mostly associated with immune response and inflammatory mechanisms. The enrichment of the Salmon module demonstrated 19 GO terms and 21 KEGG pathways, related to muscle energy metabolism, lipid metabolism, muscle degradation, and oxidative stress diseases. The Ivory and Light yellow modules have not shown significant results in the enrichment analysis. CONCLUSION: With this study, we verified that inflammation and immune response pathways modulate the BFT trait. Energy and lipid metabolism pathways, highlighting fatty acid metabolism, were the central pathways associated with REA. Some genes, as RSAD2, EIF2AK2, ACAT1, and ACSL1 were considered as putative candidate related to these traits. Altogether these results allow us to a better comprehension of the molecular mechanisms that lead to muscle and fat deposition in bovine.

Comparative muscle transcriptome associated with carcass traits of Nellore cattle.

BACKGROUND: Commercial cuts yield is an important trait for beef production, which affects the final value of the products, but its direct determination is a challenging procedure to be implemented in practice. The measurement of ribeye area (REA) and backfat thickness (BFT) can be used as indirect measures of meat yield. REA and BFT are important traits studied in beef cattle due to their strong implication in technological (carcass yield) and nutritional characteristics of meat products, like the degree of muscularity and total body fat. Thus, the aim of this work was to study the Longissimus dorsi muscle transcriptome of Nellore cattle, associated with REA and BFT, to find differentially expressed (DE) genes, metabolic pathways, and biological processes that may regulate these traits. RESULTS: By comparing the gene expression level between groups with extreme genomic estimated breeding values (GEBV), 101 DE genes for REA and 18 for BFT (false discovery rate, FDR 10%) were identified. Functional enrichment analysis for REA identified two KEGG pathways, MAPK (Mitogen-Activated Protein Kinase) signaling pathway and endocytosis pathway, and three biological processes, response to endoplasmic reticulum stress, cellular protein modification process, and macromolecule modification. The MAPK pathway is responsible for fundamental cellular processes, such as growth, differentiation, and hypertrophy. For BFT, 18 biological processes were found to be altered and grouped into 8 clusters of semantically similar terms. The DE genes identified in the biological processes for BFT were ACHE, SRD5A1, RSAD2 and RSPO3. RSAD2 has been previously shown to be associated with lipid droplet content and lipid biosynthesis. CONCLUSION: In this study, we identified genes, metabolic pathways, and biological processes, involved in differentiation, proliferation, protein turnover, hypertrophy, as well as adipogenesis and lipid biosynthesis related to REA and BFT. These results enlighten some of the molecular processes involved in muscle and fat deposition, which are economically important carcass traits for beef production.

Prediction of retail beef yield and fat content from live animal and carcass measurements in Nellore cattle.

Data from 156 Nellore males were used to develop equations for the prediction of retail beef yield and carcass fat content, expressed as kilograms and as a percentage, from live animal and carcass measurements. Longissimus muscle area and backfat and rump fat thickness were measured by ultrasound up to 5 d before slaughter and fasted live weight was determined 1 d before slaughter. The same traits were obtained after slaughter. The carcass edible portion (CEP in kg and CEP% in percentage; n = 116) was calculated by the sum of the edible portions of primal cuts: hindquarter, forequarter, and spare ribs. Trimmable fat from the carcass boning process, with the standardization of about 3 mm of fat on retail beef, was considered to be representative of carcass fat content. Most of the variation in CEP was explained by fasted live weight or carcass weight (R(2) of 0.92 and 0.96); the same occurred for CEP% (R(2) of 0.15 and 0.13), and for CEP, the inclusion of LM area and fat thickness reduced the equation bias (lower value of Mallow's Cp statistics). For trimmable fat, most variation could be explained by weight or rump fat thickness. In general, the equations developed from live animal measurements showed a predictive power similar to the equations using carcass measurements. In all cases, the traits expressed as kilograms were better predicted (R(2) of 0.39 to 0.96) than traits expressed as a percentage (R(2) of 0.08 to 0.42).

Desempenho e características da carcaça de bovinos alimentados com dietas com caroço de algodão / Performance and carcass traits of steers fed diets containing whole cottonseed

Avaliou-se o efeito da adição de caroço de algodão à dieta de bovinos confinados sobre o desempenho animal e características da carcaça. Usaram-se 36 novilhos Nelore com médias de idade e peso vivo inicial de 20 meses e 333,5kg. Os animais foram confinados e receberam dietas com os seguintes teores de caroço de algodão: 0; 14,35 por cento; 27,51 por cento; e 34,09 por cento na matéria seca da dieta. O ganho de peso diário, peso vivo final e consumo de matéria seca diminuíram linearmente com o aumento da proporção de caroço de algodão na dieta. Não foi observado efeito sobre o ganho de peso total ajustado ao consumo. Os pesos da carcaça e do contrafilé diminuíram linearmente com o aumento da proporção de caroço de algodão na dieta. Não foi observado efeito do teor de caroço de algodão sobre a área de olho de lombo. A espessura de gordura de cobertura do dorso e da garupa diminuíram com o aumento do teor de caroço de algodão. Observou-se correlação de 0,62 entre as duas medidas de gordura. A adição de caroço de algodão à dieta de bovinos de corte não se mostrou vantajosa, pois diminuiu o desempenho animal e a deposição de gordura na carcaça.

The purpose of this experiment was to evaluate the effect of adding whole cottonseed to the diet of feedlot cattle over the performance and carcass traits. Thirty six Nellore bullocks were used, with an average age of 20 months and average initial weight of 333.5kg. The animals were raised on feedlot and fed diets with the following content of whole cottonseed: 0; 14.35 percent; 27.51 percent; or 34.09 percent on a dry matter basis. The daily weight gain, final weight and dry matter intake decreased linearly as the proportion of cottonseed increased. No effect of cottonseed on total weight gain adjusted for intake was observed. Carcass and ribeye weights decreased linearly as cottonseed content increased. No effect of cottonseed content was observed for ribeye area. In contrast, back fat and rump fat thickness decreased as cottonseed content increased. A correlation of .62 was observed between back fat and rump fat. Adding whole cottonseed to beef cattle diets did not prove advantageous because it reduced animal performance and carcass fat deposition.