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1.
J Pers Med ; 14(6)2024 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-38929873

RESUMO

Anterior cruciate ligament (ACL) instability poses a considerable challenge in traumatology and orthopedic medicine, demanding precise diagnostics for optimal treatment. The pivot-shift test, a pivotal assessment tool, relies on subjective interpretation, emphasizing the need for supplementary imaging. This study addresses this limitation by introducing a machine learning classification algorithm integrated into a mobile application, leveraging smartphones' built-in inertial sensors for dynamic rotational stability assessment during knee examinations. Orthopedic specialists conducted knee evaluations on a cohort of 52 subjects, yielding valuable insights. Quantitative analyses, employing the Intraclass Correlation Coefficient (ICC), demonstrated robust agreement in both intraobserver and interobserver assessments. Specifically, ICC values of 0.94 reflected strong concordance in the timing between maneuvers, while signal amplitude exhibited consistency, with the ICC ranging from 0.71 to 0.66. The introduced machine learning algorithms proved effective, accurately classifying 90% of cases exhibiting joint hypermobility. These quantifiable results underscore the algorithm's reliability in assessing knee stability. This study emphasizes the practicality and effectiveness of implementing machine learning algorithms within a mobile application, showcasing its potential as a valuable tool for categorizing signals captured by smartphone inertial sensors during the pivot-shift test.

2.
Food Sci Technol Int ; : 10820132241248675, 2024 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-38689461

RESUMO

In recent years, people have become interested in consuming low-fat foods as this reduces the risk of obesity and cardiovascular disease. For this reason, this study optimized the incorporation of passion fruit epicarp (PFE) as a partial fat substitute in dairy ice cream. For this purpose, a central composite rotational design 22 was performed and 5 response variables were optimized, of which 4 variables were maximized (percentage overrun, hardness, adhesiveness, and color coordinates L*, a*, b*), while the melting rate was minimized as a function of two factors (% PFE and % fat). The stability of three types of ice cream was then evaluated: experimental ice cream (3% milk fat and 0.97% PFE), commercial ice cream (4% milk fat and 2% vegetable fat) and control ice cream (3% milk fat without PFE added), according to their physicochemical and sensory properties during 57 days of storage (-22 °C, 85% relative humidity). The results showed that it was possible to reduce the milk fat by up to 25% by adding PFE (0.97%), which significantly reduced the melting rate and resulted in higher hardness (N), adhesiveness and increased yellowness compared to the commercial samples. The optical microscopy analysis also showed that PFE keeps the air bubbles immobile due to the increased viscosity in the serum phase during the 57-day storage.

3.
J Thromb Thrombolysis ; 57(4): 721-729, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38523179

RESUMO

Hypercoagulability and reduced fibrinolysis are well-established complications associated with COVID-19. However, the timelines for the onset and resolution of these complications remain unclear. The aim of this study was to evaluate, in a cohort of COVID-19 patients, changes in coagulation and fibrinolytic activity through ROTEM assay at different time points during the initial 30 days following the onset of symptoms in both mild and severe cases. Blood samples were collected at five intervals after symptoms onset: 6-10 days, 11-15 days, 16-20 days, 21-25 days, and 26-30 days. In addition, fibrinogen, plasminogen, PAI-1, and alpha 2-antiplasmin activities were determined. Out of 85 participants, 71% had mild COVID-19. Twenty uninfected individuals were evaluated as controls. ROTEM parameters showed a hypercoagulable state among mild COVID-19 patients beginning in the second week of symptoms onset, with a trend towards reversal after the third week of symptoms. In severe COVID-19 cases, hypercoagulability was observed since the first few days of symptoms, with a tendency towards reversal after the fourth week of symptoms onset. A hypofibrinolytic state was identified in severe COVID-19 patients from early stages and persisted even after 30 days of symptoms. Elevated activity of PAI-1 and alpha 2-antiplasmin was also detected in severe COVID-19 patients. In conclusion, both mild and severe cases of COVID-19 exhibited transient hypercoagulability, reverted by the end of the first month. However, severe COVID-19 cases sustain hypofibrinolysis throughout the course of the disease, which is associated with elevated activity of fibrinolysis inhibitors. Persistent hypofibrinolysis could contribute to long COVID-19 manifestations.


Assuntos
Antifibrinolíticos , COVID-19 , Trombofilia , Humanos , Fibrinólise , Inibidor 1 de Ativador de Plasminogênio/farmacologia , Síndrome de COVID-19 Pós-Aguda
4.
Front Pharmacol ; 14: 1176629, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37886132

RESUMO

Amazonian flora includes several species with the potential to develop pharmaceutical and biotechnological products. The essential oils from Amazonian species possess some biological properties, such as antioxidant, antibacterial, and cytotoxic activities. The essential oil of red sacaca (RSO), Croton cajucara Benth., contains metabolites characterized by antioxidant and anti-inflammatory activities. Nanostructured lipid carriers (NLC) are an advantageous alternative for the effective delivery of drugs because they can solubilize lipophilic actives and reduce their cytotoxicity. This study aimed to optimize the synthesis of RSO-loaded nanostructured lipid carriers (NLC-RSO) using a 23 factorial design and investigate their antioxidant and cytotoxic effects. The red sacaca essential oil (RSO) metabolite profile was characterized using gas chromatography coupled with a mass spectrometer (GC-MS), identifying 33 metabolites, with linalool and 7-hydroxy-calamenene as the major ones, as reported in the literature. The optimized NLC-RSO formulation had a particle size less than 100 nm and a polydispersity index lower than 0.25. After characterizing NLC-RSO using Fourier-transform infrared spectroscopy, powder X-ray diffraction, zeta potential, moisture content, and wettability, in vitro cytotoxicity were performed in A549 and BEAS-2B cell lines using the resazurin metabolism assay. The data indicated a lower IC50 for RSO than for NLC-RSOs in both cell lines. Furthermore, low cytotoxicity of blank nanoparticles (blank NP) and medium chain triglycerides-loaded nanostructured lipid carriers (NLC-MCT) towards both pulmonary cell lines was noted. At a concentration of 50-100 µg/mL, free RSO exhibited higher cytotoxicity than NLC-RSO, demonstrating the protective effect of this lipid carrier in reducing cytotoxicity during metabolite delivery. Similarly, free RSO showed higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging than NLC-RSO, also indicating this protective effect. The 2',7'-dichlorofluorescein diacetate (DCFH-DA) intracellular reactive oxygen species (ROS) level assay did not show differences between the treatments at higher but non-cytotoxic dosages. Taken together, our results suggest that NLC-RSOs are potential RSO delivery systems for applications related to cancer treatment.

5.
Heliyon ; 9(9): e19990, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37809611

RESUMO

The design of structures submitted to aerodynamic loads usually requires the development of specific computational models considering fluid-structure interactions. Models using structural frame elements are developed in several relevant applications such as, the design of advanced aircraft wings, wind turbine blades or power transmission lines. In the case of flexible frame structures submitted to fluid flows, the computation of inertial and aerodynamic forces for large displacements and rotations is a challenging task. In this article, we present a novel formulation for the efficient computation of aerodynamic forces in frame structures, coupling the co-rotational framework with the quasi-steady theory. A numerical procedure is provided considering a tangent matrix for the aerodynamic forces. This formulation is implemented in the open-source library ONSAS, allowing users to reproduce the results or solve other frame nonlinear dynamic problems. The proposed formulation and its implementation are validated through the resolution of four numerical examples. The formulation and the numerical procedure proposed efficiently provide accurate solutions for these challenging problems with large displacements and rotations.

6.
Toxicon ; 234: 107301, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37741576

RESUMO

Venom-induced consumption coagulopathy and thrombocytopenia are common and potentially severe manifestations of viperid snakebite envenoming since they contribute to local and systemic hemorrhage. Therefore, the assessment of the efficacy of antivenoms to neutralize coagulopathic and thrombocytopenic toxins should be part of the preclinical evaluation of these drugs. To evaluate the efficacy of the polyvalent (Crotalinae) antivenom produced in Costa Rica, in this study we have used a mouse model of coagulopathy and thrombocytopenia induced by the venom of Bothrops asper, based on the bolus intravenous (i.v.) injection of venom. When venom and antivenom were incubated before injection, or when antivenom was administered i.v. immediately after venom injection, venom-induced hemostatic alterations were largely abrogated. We also studied the recovery rate of clotting parameters in conditions where antivenom was administered when mice were coagulopathic. Some parameters recovered more rapidly in antivenom-treated mice than in control envenomed animals, but others showed a spontaneous recovery without antivenom. This is due to a rapid clearance of plasma venom levels in these experimental conditions. This implies that models based on the bolus i.v. injection of venom have limitations for assessing the effect of antivenom in the recovery of clotting alterations once coagulopathy has developed. It is suggested that alternative models should be developed based on a slower systemic absorption of venom. Overall, our findings provide a protocol for the preclinical evaluation of antivenoms and demonstrate that the polyvalent antivenom is effective in neutralizing the toxins of B. asper venom responsible for coagulopathy and thrombocytopenia.

7.
Toxicol Ind Health ; 39(10): 583-593, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37530424

RESUMO

Textile effluents, although their composition can vary considerably, typically contain high levels of dissolved salts and exhibit wide variations in pH. Ecotoxicological studies regarding the effects of these parameters, however, have been limited owing to the need for sensitive and easy-to-handle bioindicators that require low amounts of sampling, are cost-effective, time-efficient, and ethically endorsed. This kind of study, additionally, demands robust multi-factorial statistical designs that can accurately characterize the individual and combined relationship between variables. In this research, Response Surface Methodology (RSM) was used to calculate the individual and interaction effects of NaCl concentration and pH value of a Simulated Textile Effluent (STE) on the development rate (DR) of the bioindicators: Bacillus subtilis bacteria and Lactuca sativa lettuce. The results demonstrated that the bioindicators were sensitive to both NaCl and pH factors, where the relative sensitivity relationship was B. subtilis > L. sativa. The quadratic equations generated in the experiments indicated that increased concentrations of 50-250 mg L-1 of NaCl caused a perturbance of 1.40%-34.40% on the DR of B. subtilis and 0.50%-12.30% on L. sativa. The pH factor at values of 3-11 caused an alteration of 27.00%-64.78% on the DR of the B. subtilis and 51.37%-37.37% on the L. sativa. These findings suggest that the selected bioindicators could serve as effective tools to assess the ecotoxicological effects of textile effluents on different ecological systems, and the RSM was an excellent tool to consider the ecotoxicological effects of the parameters and to describe the behavior of the results. In conclusion, the NaCl and pH factors may be responsible for disrupting different ecosystems, causing imbalances in their biodiversity and biomass. Before discharge or reuse, it is suggested to remove salts and neutralize pH from textile effluents and, mostly, develop novel, eco-friendlier textile processing techniques.


Assuntos
Bacillus subtilis , Poluentes Químicos da Água , Lactuca , Cloreto de Sódio/toxicidade , Cloreto de Sódio/análise , Ecossistema , Biomarcadores Ambientais , Sais/análise , Concentração de Íons de Hidrogênio , Têxteis , Indústria Têxtil , Poluentes Químicos da Água/análise , Resíduos Industriais/análise
8.
J Pediatr ; 263: 113645, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37517648

RESUMO

OBJECTIVE: To assess serial myocardial performance and pulmonary vascular resistance (PVR) in infants of mothers with gestational diabetes mellitus (GDM) over the first year of life. STUDY DESIGN: This was a prospective, observational study. Echocardiography was performed at birth, 6 months, and 1 year of age. Pulmonary artery acceleration time and left ventricular (LV) eccentricity index provided surrogate measurements of PVR. Biventricular function was assessed by tissue Doppler imaging and deformation analysis. RESULTS: Fifty infants of mothers with GDM were compared with 50 controls with no difference in gestation (38.9 ± 0.8 weeks vs 39.3 ± 0.9 weeks; P = .05) or birthweight (3.55 ± 0.49 kg vs 3.56 ± 0.41 kg; P = .95). At 1 year of age, the pulmonary artery acceleration time was lower (70 ± 11 vs 79 ± 10; P = .01) in the GDM group. LV global longitudinal strain (24.7 ± 1.9 vs 28.8 ± 1.8 %; P < .01), LV systolic strain rate (1.8 ± 0.2 vs 2.1 ± 0.3 1/s; P < .01), and RV free wall strain (31.1 ± 4.8 vs 34.6 ± 3.9 %; P < .01) were lower in the GDM cohort at 1 year of age (all P values adjusted for gestation, mode of delivery, and maternal body mass index). CONCLUSIONS: Our findings demonstrate higher indices of PVR and lower biventricular function in infants of mothers with GDM compared with controls at each time point assessed in this study over the first year of life.


Assuntos
Diabetes Gestacional , Gravidez , Recém-Nascido , Feminino , Humanos , Lactente , Diabetes Gestacional/diagnóstico por imagem , Estudos Prospectivos , Ecocardiografia/métodos , Miocárdio , Sístole , Idade Gestacional
9.
Food Sci Technol Int ; : 10820132231172363, 2023 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-37128651

RESUMO

Pectin methylesterase (PME) is the target-enzyme in orange juice processing, and its inactivation preserves the original juice's cloud, a valuable quality attribute for consumers. This study was primarily undertaken to evaluate the combination of supercritical carbon dioxide (SC-CO2) and mild temperatures on PME inactivation in the juice. Physicochemical and color parameters were also evaluated. Pressures (P) in the range of 74 to 351 bar, temperatures (T) between 33°C and 67°C, and holding times (t) varying from 20 to 70 min were tested using a central composite rotational design. Determination of PME activity, pH, titratable acidity, soluble solids, ratio, lightness, °hue and chroma in freshly extracted/raw and treated juice samples, and total color difference (TCD) between raw and treated juice were carried out. The percentage of PME reduction widely ranged from 13.7% to 91.8%. The variable P had a significant effect (p ≤ 0.05) on TCD and acidity variation. T exhibited a significant effect on the PME reduction, TCD, and soluble solids variation; however, t impacted on the soluble solids variation only. The interaction between P and t (P.t) affected the soluble solids and acidity variation, and T.t affected TCD. The combination of SC-CO2 and mild temperatures is potentially capable of inactivating PME in orange juice.

10.
Anal Chim Acta ; 1266: 341354, 2023 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-37244664

RESUMO

BACKGROUND: the chemometric processing of second-order chromatographic-spectral data is usually carried out with the aid of multivariate curve resolution-alternating least-squares (MCR-ALS). When baseline contributions occur in the data, the background profile retrieved with MCR-ALS may show abnormal lumps or negative dips at the position of the remaining component peaks. RESULTS: The phenomenon is shown to be due to remaining rotational ambiguity in the obtained profiles, as confirmed by the estimation of the boundaries of the range of feasible bilinear profiles. To avoid the abnormal features in the retrieved profile, a new background interpolation constraint is proposed and described in detail. Both simulated and experimental data are employed to support the need of the new MCR-ALS constraint. In the latter case, the estimated analyte concentrations agreed with those previously reported. SIGNIFICANCE: The developed procedure helps to reduce the extent of rotational ambiguity in the solution and to better interpret the results on physicochemical grounds.

11.
Environ Technol ; : 1-20, 2023 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-36927407

RESUMO

Using the Response Surface Methodology (RSM) and Rotational Central Composite Design (RCCD), this study evaluated the removal of DCF under denitrifying conditions, with ethanol as cosubstrate, in batch reactors, being 1 L Erlenmeyer flasks (330 mL of reactional volume) containing Dofing medium and kept under agitation at 130 rpm and incubated at mesophilic temperature (30 °C). It considered the individual and multiple effects of the variables: nitrate (130 - 230 mg NO3- L-1), DCF (60-100 µg DCF L-1) and ethanol (130 - 230 mg EtOH L-1). The highest drug removal efficiency (17.5%) and total nitrate removal were obtained at 176.6 ± 4.3 mg NO3 -L-1, 76.8 ± 3.7 µg DCF L-1, and 180.0 ± 2.5 mg EtOH L-1. Under such conditions, the addition of ethanol and nitrate was significant for the additional removal of diclofenac (p > 0.05). The prevalence of Rhodanobacter, Haliangium and Terrimonas in the inoculum biomass (activated sludge systems) was identified through the 16S rRNA gene sequencing. The potential of these genera to remove nitrate and degrade diclofenac was inferred, and the main enzymes potentially involved in this process were α-methylacyl-CoA racemase, long-chain fatty acid-CoA ligase, catalases and pseudoperoxidases.

12.
Childs Nerv Syst ; 39(6): 1647-1651, 2023 06.
Artigo em Inglês | MEDLINE | ID: mdl-36705687

RESUMO

INTRODUCTION: This study aimed to highlight that atlantoaxial rotatory fixation (AARF) can be related to neurosurgery procedures in children, with an afterward demonstration of good results after halo-gravity traction and C1-C2 stabilization using the Harms technique. CASE DESCRIPTION: To the best of our knowledge, this is the first case to report a 4-year-old boy who presented with AARF after a posterior fossa craniotomy to treat a cerebellar astrocytoma. At our medical facility, AARF was diagnosed using plain radiography and computed tomography imaging. The patient was treated with continuous cranial traction for 14 days. Initially, we detected that the patient had no C1 posterior arch or C2 spinous process; therefore, the best option was to perform the Harms technique. Postoperatively, the patient was placed in a cervical collar for 4 weeks. At the 4-year postoperative follow-up, the patient was doing well and had not developed any complications. CONCLUSION: Herein, we report a case in which AARF can be developed after neurosurgical procedure. Surgical techniques used for atlantoaxial subluxation should be carefully selected. In our case, the Harms technique after cranial traction was an excellent option for correcting and stabilizing the abnormal neck position. However, further studies are required to determine the best technique to use in the pediatric population.


Assuntos
Articulação Atlantoaxial , Luxações Articulares , Masculino , Humanos , Criança , Pré-Escolar , Articulação Atlantoaxial/diagnóstico por imagem , Articulação Atlantoaxial/cirurgia , Luxações Articulares/complicações , Radiografia , Tomografia Computadorizada por Raios X , Craniotomia
13.
J Blood Disord, v. 10, n. 1, 1074, jun. 2023
Artigo em Inglês | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP | ID: bud-5371

RESUMO

Background: Sensitivity of classical coagulation assays by using mammalian plasmas to pro‐ and anticoagulant compounds includ ing venom or toxins occurs on a microscale level (micrograms). Al though it improves responses to agonists, recalcification triggers a relatively fast thrombin formation process. The Recalcification Time (RT) of factor XII- deficient Chicken Plasma (CP) is comparatively long (≥1800 seconds) when compared to human plasma or others. Our objective was to compare its sensitivity with that presented by human plasma samples to Unfractionated Heparin (UH), a pro totype anticoagulant compound, under similar conditions through rotational thromboelastometry. Methods: To find doses of UH sufficient enough to prolong the Clotting Time (CT) parameter of these activated plasmas to values within their normal RT ranges. Results: In total, 0.0065±0.0009 IU of UH (n=6) was detected in 260µL of CP samples, but only 0.125±0.012 IU of UH was sufficient to induce a similar effect in activated human plasma samples. Conclusion: The higher sensitivity of CP to anticoagulants could be useful for (a) detection of anticoagulant compounds in substanc es of unknown origin; (b) purification procedures of anticoagulant toxins from crude animal venoms and (c) determination of relative potencies of agonists and their selective antagonists such as phar maceutical agents, antivenoms or natural inhibitors of venom tox ins with a better result in kinetic clothing parameters

14.
Rev. bras. oftalmol ; 82: e0029, 2023. graf
Artigo em Português | LILACS-Express | LILACS | ID: biblio-1449768

RESUMO

RESUMO Objetivo Avaliar a precisão da tomografia corneana com imagens de Scheimpflug (Pentacam® AXL, OCULUS, Wetzlar, Alemanha) nos padrões de escaneamento com 25 e 50 imagens, verificando sua repetibilidade e reprodutibilidade em olhos normais, com ceratocone e com ceratocone após crosslinking. Métodos Estudo prospectivo, transversal, misto, no qual foram incluídos os pacientes que tinham córneas normais, ou com ceratocone; e não tinham realizado cirurgias corneanas, com exceção do crosslinking. Foram excluídos os pacientes que tivessem realizado outras cirurgias, como implante de anel intracorneano ou transplante de córnea. Foram realizadas três medidas com 25 imagens e três medidas com 50 imagens em cada olho incluído no estudo. Resultados O estudo avaliou 54 olhos de 41 pacientes. Destes, dez (18,52%) olhos eram com córneas normais e sem cirurgia ocular prévia aleatoriamente selecionados de 5 pacientes; 22 (40,74%) olhos aleatoriamente selecionados de 18 pacientes com ceratocone, sem cirurgia ocular prévia; e 22 (40,74%) olhos com ceratocone de 18 pacientes operados por crosslinking. No estudo da repetibilidade, percebemos uma maior variação de valores em todos os índices nos pacientes com ceratocone comparados aos de pacientes com olhos normais, em grande parte dos parâmetros analisados. Conclusão Não houve diferença significativa entre os métodos de escaneamento por 25 e 50 imagens, sendo o escaneamento por 25 imagens mais conveniente. A presença de ceratocone teve impacto negativo na repetibilidade de ambos os tipos de exame, e foi grande a concordância entre os métodos nos três grupos estudados. O coeficiente de repetibilidade permite considerar a relevância clínica dos dados para avaliar a progressão de ceratocone.


ABSTRACT Objective To evaluate the accuracy of corneal tomography with Scheimpflug images (Pentacam® AXL, OCULUS, Wetzlar, Germany) in scanning patterns with 25 and 50 images, by analyzing their repeatability and reproducibility in normal eyes, eyes with keratoconus and with keratoconus after crosslinking. Methods Prospective, cross-sectional, mixed study including patients who had normal corneas, or who had keratoconus, who had not undergone corneal surgery, with the exception of crosslinking. Patients who had undergone surgery for intracorneal ring implantation or corneal transplantation were excluded. Three measurements were performed with 25 images and three measurements were performed with 50 images on each eye included in the study. Results The study evaluated a total of 54 eyes of 41 patients. Of these, 10 (18.52%) eyes had normal corneas, without previous eye surgery, 22 (40.74%) were eyes with keratoconus, without previous eye surgery, and 22 (40.74%) were eyes with keratoconus, with crosslinking for keratoconus. In the study of repeatability, we noticed a greater variation of values in all indices in patients with KC compared to those with normal eyes, in most of the parameters analyzed. Conclusion There is no significant difference between the scanning methods with 25 and 50 images. However, 25 scans has advantages that make it the main method suggested. The presence of keratoconus has a negative impact on the repeatability of both types of examination, and there is great agreement between the methods in the three groups studied. The high repeatability coefficient in some parameters suggests that these data should be used with care in the assessment of keratoconus progression.

15.
Foods ; 11(23)2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-36496669

RESUMO

The application of ß-galactosidase in the fermentation of milk enables the acquirement of lower levels of lactose that are tolerated by lactose maldigesters and can reduce the nutritional consequences of avoiding dairy products. The present study evaluated the viability of the fortification of lactose-free prebiotic Greek yogurt formulas with whey protein concentrate (WPC). Two rotational central composite designs (RCCDs) were applied: one to perform the hydrolysis of the whey protein concentrate and another for the yogurt formulations (α = 2 with 2 central points and 4 axial points). Two ß-galactosidase enzymes obtained from Kluyveromyces lactis were used. The content of lactose, glucose, galactose, and lactic acid were determined in the WPC, milk (pasteurized and powdered), and yogurts. The three best formulations regarding the attributes' viscosity, syneresis, firmness, and elasticity were sensorially evaluated by using a nine-point hedonic scale. A microbiological analysis was performed after 48 h of yogurt production. The characterization of the products and the comparison of the results obtained were evaluated using the Student's T test and the analysis of variance with Tukey's test (p-values < 0.05). The application of a lactose-free WPC promoted viscosity, firmness, and elasticity. The syneresis was reduced, and whey increased the protein and calcium content. Lactose-free WPC can be used as a partial substitute for skimmed powdered milk in yogurts. The obtained results are encouraging with respect to the production of lactose-free Greek yogurts by the dairy industry.

16.
Environ Technol ; : 1-15, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36352347

RESUMO

ABSTRACTNonylphenol ethoxylate (NPE) is an endocrine-disrupting chemical that has bioaccumulative, persistent and toxic characteristics in different environmental matrices and is difficult to remove in sewage treatment plants. In this study, the effects of the initial concentration of NPE (0.2 ± 0.03 - 3.0 ± 0.02 mg. L-1) and ethanol (73.9 ± 5.0-218.6 ± 10.6 mg. L-1) were investigated using factorial design. Assays were carried out in anaerobic batch reactors, using the Zinder basal medium, yeast extract (200 mg. L-1), vitamin solution and sodium bicarbonate (10% v/v). The optimal conditions were 218.56 mg.L-1 of ethanol and 1596.51 µg.L-1 of NPE, with 92% and 88% of NPE and organic matter removal, respectively, and methane yield (1689.8 ± 59.6 mmol) after 450 h of operation. In this condition, bacteria potentially involved in the degradation of this surfactant were identified in greater relative abundance, such as Acetoanaerobium (1.68%), Smithella (1.52%), Aminivibrio (0.91%), Petrimonas (0.57%) and Enterobacter (0.47%), as well as archaea Methanobacterium and Methanoregula, mainly involved in hydrogenotrophic pathway.

17.
Sensors (Basel) ; 22(20)2022 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-36298314

RESUMO

Computer vision techniques can monitor the rotational speed of rotating equipment or machines to understand their working conditions and prevent failures. Such techniques are highly precise, contactless, and potentially suitable for applications without massive setup changes. However, traditional vision sensors collect a significant amount of data to process and measure the rotation of high-speed systems, and they are susceptible to motion blur. This work proposes a new method for measuring rotational speed processing event-based data applied to high-speed systems using a neuromorphic sensor. This sensor produces event-based data and is designed to work with high temporal resolution and high dynamic range. The main advantages of the Event-based Angular Speed Measurement (EB-ASM) method are the high dynamic range, the absence of motion blurring, and the possibility of measuring multiple rotations simultaneously with a single device. The proposed method uses the time difference between spikes in a Kernel or Window selected in the sensor frame range. It is evaluated in two experimental scenarios by measuring a fan rotational speed and a Router Computer Numerical Control (CNC) spindle. The results compare measurements with a calibrated digital photo-tachometer. Based on the performed tests, the EB-ASM can measure the rotational speed with a mean absolute error of less than 0.2% for both scenarios.


Assuntos
Movimento , Movimento (Física) , Monitorização Fisiológica
18.
Materials (Basel) ; 15(17)2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36079479

RESUMO

Despite geopolymers having emerged as a more sustainable alternative to Portland cement, their rheological properties still need to be thoroughly investigated, aiming at the material's applicability. Additionally, studies that evaluated the fresh state of geopolymer composites with nanomaterials are scarce. Thus, two metakaolin-based geopolymer systems were reinforced with nanomaterials with a similar geometry: carbon nanotubes (CNT) and silicon carbide whiskers (SCW). The nanomaterials incorporation was assessed by rotational rheometry (conducted up to 110 min), isothermal calorimetry, compressive strength after 7 and 28 days, and the microstructure was investigated using X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR). CNT and SCW incorporation (0.20 wt.%) did not significantly affect the yield stress and viscosity of the R2-group (based on metakaolin type 2), while increasing the rheological parameters up to 56.0% for the R1-group (based on metakaolin type 1). Both additions modified the reaction kinetics. Increments of up to 40.7% were observed in the compressive strength of geopolymer pastes with the incorporation of a SCW content of 0.2 wt.%. XRD and FTIR results suggest similar structural modifications between precursors. Nevertheless, R2 showed substantial transformations while the R1 group exhibited anhydrous material that can react over time. Overall, incorporating CNT and SCW contributed to higher mechanical increments on systems with average mechanical strength (R1) compared to systems with higher potential mechanical performance (R2).

19.
Braz J Microbiol ; 53(4): 1871-1880, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36173601

RESUMO

Microencapsulation of microorganisms has been studied to increase product shelf life and stability to enable the application in sustainable agriculture. In this study, the microencapsulation of Trichoderma asperellum conidia by spray drying (SD) was evaluated. The objective was to assess the effect of drying air temperature and wall material (maltodextrin DE20, MD20) concentration on the microencapsulation and to identify the optimum conditions to produce, in high yield, microparticles with low moisture, high conidial viability and conidial survival. Microparticles were characterized in terms of morphology, particle size, and shelf life. A central composite rotatable design (CCRD) was used to evaluate the effect of operating parameters on drying yield (DY), moisture content, conidial viability (CV), and conidial survival (SP). Microencapsulation experiments were carried out under optimum conditions to validate the obtained model. The optimum temperature and MD20/conidia dry weight ratios were 80 °C and 1:4.5, respectively, which afforded a drying yield of 63.85 ± 0.86%, moisture content of 4.92 ± 0.07%, conidial viability of 87.10 ± 1.16%, and conidial survival of 85.78 ± 2.88%. Microencapsulation by spray drying using MD20 as wall material extended the viability of conidia stored at 29 °C compared with the control. The mathematical models accurately predicted all the variables studied, and the association of the microencapsulation technique using DE20 maltodextrin was able to optimize the process and increase the product's shelf life. It was also concluded that high inlet air temperatures negatively affected conidia survival, especially above 100 °C.


Assuntos
Hypocreales , Secagem por Atomização , Esporos Fúngicos , Dessecação
20.
Rev. mex. anestesiol ; 45(3): 184-187, jul.-sep. 2022. tab, graf
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1409784

RESUMO

Resumen: La tromboelastometría evalúa los cambios viscoelásticos en el proceso de coagulación. Nos ofrece una representación gráfica de la formación del coágulo, la estabilidad del mismo y la presencia de lisis. La tromboelastometría rotacional es una herramienta diagnóstica que representa de forma gráfica la funcionalidad del coágulo para un manejo dirigido e individualizado de la coagulopatía asociada a hemorragia. En este trabajo se puntualiza cómo la tromboelastometría rotacional es a la coagulación como el electrocardiograma es al corazón.


Abstract: Thromboelastometry evaluates viscoelastic changes in the coagulation process. It offers us a graphic representation of the formation of the clot, its stability and the presence of lysis. Rotational thromboelastometry is a diagnostic tool that graphs the functionality of the clot, for a targeted and individualized management of bleeding-associated coagulopathy. In this work it is specified how rotational thromboelastometry is to coagulation as the electrocardiogram is to the heart.

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