Bacterial Flora in Screw-Fixed Superstructures with Different Sealing Materials: A Comparative Clinical Trial.

A screw-fixed superstructure is predominantly selected for implant prostheses because of the concern regarding developing peri-implantitis, although its infection route remains unclear. Focusing on microleakage from access holes, the present study clinically investigated the bacterial flora in access holes with different sealing materials. We examined 38 sites in 19 patients with two adjacent screw-fixed superstructures. Composite resin was used in the control group, and zinc-containing glass ionomer cement was used in the test group. Bacteria were collected from the access holes 28 days after superstructure placement and were subjected to DNA hybridization analysis. The same patient comparisons of the bacterial counts showed a significant decrease in 14 bacterial species for the red, yellow, and purple complexes in the test group (p < 0.05). In addition, the same patient comparisons of the bacterial ratios showed a significant decrease in six bacterial species for the orange, green, yellow, and purple complexes in the test group (p < 0.05). Furthermore, the same patient comparisons of the implant positivity rates showed a significant decrease in the six bacterial species for the orange, yellow, and purple complexes in the test group. The results of this study indicate that zinc-containing glass ionomer cement is effective as a sealing material for access holes.

Evaluation of sealing efficacy and removal convenience of sealing materials for implant abutment screw access holes.

BACKGROUND: Sealing materials are used to fill abutment screw access holes (SAH) to prevent microleakage and protect the central screws in oral implant restoration. However, thus far, no consensus has been reached on sealing material selection. In this study, a comparison of the sealing efficacy and removal convenience of different sealing materials for cement-retained implant restoration was conducted. METHODS: Various sealing materials were classified into five groups, namely, gutta-percha (GP), temporary restorative paste (TRP), vinyl polysiloxane (VPS), polytetrafluoroethylene (PTFE) tape, and onlay resin (OR), and 35 sets of analog-abutments were allocated into five groups of seven specimens. A sealing efficacy test was conducted using a modified dye-penetration method, in which a lower absorbance indicated better sealing efficacy. For the removal-convenience test, the materials were removed from each SAH after solidification, and the retrieval time was recorded. RESULTS: On days 1 and 10, PTFE exhibited the highest absorbance value with significant differences compared to the other groups. On day 30, TRP and PTFE showed significantly higher absorbance values than GP, VPS, and OR, but no significant difference was detected between TRP and PTFE (p = 0.424). The absorbance values of TRP and PTFE from days 1, 10, and 30 showed significant intragroup differences, while those of the other groups did not. In terms of the removal convenience on days 1, 10, and 30, VPS achieved the best performance, followed by PTFE, OR, TRP, and GP. CONCLUSION: Within the limitations of this experiment, VPS and OR showed better sealing efficacy against microleakage and a more convenient removal than the other materials; thus, VPS and OR are recommended for clinical use.

The Use of the Diode Laser against the Microbiome on Composites Closing the Screw Access Hall (Sah) in the Reconstruction of Dental Implants: Ex Vivo Studies.

Patients undergoing implant treatment are at risk of peri-implant bone loss, which is most often caused by the adverse effects of microorganisms, but there are few proven procedures for their reduction. The aim of the research was to identify the microorganisms inhabiting the composites used to close the screw access hole (SAH), compare them numerically with those present on the surface of crowns and teeth, and optimize the doses of the diode laser, which will reduce microorganisms and will not deteriorate the roughness of polished composites. Patients were swabbed from the surface of SAH composites, from porcelain and zirconium restorations, and from teeth, and then the number of microorganisms was determined by using a culture technique. Microorganisms were identified by MALDI-TOF MS and NGS sequencing. The effectiveness of diode laser irradiation was achieved by using four variants of exposure. After polishing and laser irradiation, the surface roughness of the composites was studied by using optical profilometry. On the surface of SAH, 106 to 108 microorganisms were identified at 0.4 cm2, including many pathogenic species. Among the materials used for the reconstruction of dental implants, the greatest microbiological contamination was found on the composites used to close the SAH. The diode laser with a wavelength of 810 nm with an average power of 3.84 W, during 60 s and 2 × 30 s, has a biocidal effect and does not significantly change the surface roughness of composites. The best reduction of microorganisms was achieved on a composite polished with a polishing rubber and then with a Sof-Lex™ Pre-Polishing Spiral beige (3M ESPE, Ave. St. Paul., MN, USA). Studies have shown that using the optimal laser dose can help treat periimplantitis. These studies provide important information on the possibility of the effective elimination of microorganisms by using a diode laser in the treatment of peri-implant bone loss.

Selection of 1-mm venting or 2.5-mm screw access holes on implant crowns based on cement extrusion and retention capacity.

BACKGROUND: This in vitro study aimed to provide evidence regarding the selection of hole diameters of implant crowns to reduce excess cement extrusion at the abutment margin, and to examine the maintenance of their retention capacity in anterior and posterior cement-retained implant crowns. METHODS: Six groups of implant crowns were prepared according to the position of the teeth and the size of their holes as follows: anterior crown without hole (ANH), anterior crown with 1-mm mini venting hole (AMH), anterior crown with 2.5-mm regular screw access hole (ARH), posterior crown without hole (PNH), posterior crown with 1-mm mini venting hole (PMH), and posterior crown with 2.5-mm regular screw access hole (PRH). Temporary cement was used to bond the crowns to the abutments. The mean amount of excess cement extrusion among the different groups at the abutment margin was calculated. Retentive strength under different hole designs was measured as the dislocation force of the crown using a universal testing machine. One-way ANOVA and Welch's t-test were used to analyze the results. RESULTS: The average amounts of extruded excess cement were 18.96 ± 0.64, 1.78 ± 0.41, and 1.30 ± 0.41 mg in the ANH, AMH, and ARH groups, respectively, and 14.87 ± 0.36, 1.51 ± 0.40, and 0.82 ± 0.22 mg in the PNH, PMH, and PRH groups, respectively. The hole opening in the crowns could significantly reduce residual cement regardless of its size (p < 0.001). The mean retentive strengths were 54.16 ± 6.00, 47.63 ± 13.54, and 31.99 ± 7.75 N in the ANH, AMH, and ARH groups, respectively, and 57.84 ± 10.19, 53.22 ± 6.98, and 39.48 ± 5.12 N in the PNH, PMH, and PRH groups, respectively. The retention capacity of the implant crown deteriorated rapidly as the holes on the crown surface enlarged. CONCLUSIONS: The presence of a hole on the implant crown reduced the amount of excess cement. The retention ability of the implant crowns deteriorated as the size of the hole increased. Considering the esthetic effect of the crown and the possible influence on crown retention, an implant crown with a 1-mm mini venting hole is a better clinical choice than the one with a 2.5-mm regular screw access hole.

Periodontal Pathogen Adhesion, Cytotoxicity, and Surface Free Energy of Different Materials for an Implant Prosthesis Screw Access Hole.

Background and Objectives: Oral implant restorations are an excellent treatment option for edentulous patients; however, periodontopathogenic bacteria have been found in the microgaps between implant-abutment junctions. Implant designs to limit the microgaps have been extensively studied. However, studies have shown microgaps continue to exist, allowing for the leakage of bacteria into the implant system. Screw access hole materials are used to fill the access hole void. The use of materials with beneficial properties could provide bacterial leakage prevention. The aim of this study was to examine the surface free energy, cytotoxicity, and bacterial adhesion of selected screw access hole materials such as cotton, polytetrafluoroethylene (PTFE) tape, paraffin wax-polyolefin thermoplastic (PF), paraffin wax (Wax), gutta-percha (GP), and caviton EX (CE). Materials and Methods: A sessile drop test was performed to observe the contact angle and calculate the surface free energy of each material in order to determine the level of hydrophobicity. Cytotoxicity was examined in a mouse gingival epithelial cell line for day 1 and day 3. Bacterial adhesion was tested with Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. Results: PTFE, PF, and wax presented low surface free energies of 19.34, 23.041, and 24.883 mN.m-1, respectively. No cytotoxicity was observed, except for GP and CE. Concurrently, the bacterial adhesion was also the lowest in PTFE and PF. Conclusions: Within the limits of this study, PTFE and PF showed an excellent biocompatibility with few bacterial adhesions. These materials could be potential screw access hole materials in clinical settings.

The effect of diameter of the screw-access hole on the implant prosthodontic system and surrounding cortical bone-A 3D finite element analysis / 实用口腔医学杂志

Objective: To investigate the stress and stress distribution generated on each component of implant prosthodontic system and surrounding cortical bone when different diameters of screw-access hole (SAH) were prepared on molar crown. Methods: A fimite element(FE) model of partial mandible without first molar was set up, and an Bego implant was insert into it. A total of 5 models of the crown were computer-simulated by varying the diameter (Φ = 0-4 mm) of the SAH. The loading forces were 200 N axially (0°) and 100 N obliquely (45°) respectively on occlusive surface. The FE analysis was performed by computer. Results: Φ ≤3 mm: stress on occlusal surface of crown was almost unchanged and mainly distributed in the loading area. Φ = 4 mm, stress appeared an obvious rise and reached the maximum, the stress concentration under vertical load was changed to the hole margin. In vertical loading, screw could remain at a relatively low stress level when diameter did not exceed 1 mm. No changes on other components was observed. Conclusion: SAH diameter of 1 mm is recommended when a cement-and screw-retained crown is used in posterior region.

Influence of Sealing of the Screw Access Hole on the Fracture Resistance of Implant-Supported Restorations

Abstract The purpose of this study was to assess the influence of sealing of the screw access hole (SAH) on the fracture resistance of metal-ceramic implant-supported restorations. UCLA abutments were used to make 30 implant-retained mandibular molar restorations and divide equally into three groups: Group SRS: screw-retained restorations with SAH sealed; Group SRNS: screw-retained restorations with SAH not sealed; Group CR: cement-retained restorations. The following protocol was adopted to restore the SAH: the ceramic surface of the SAH was air-abraded with aluminum oxide; etched with 10% hydrofluoric acid; a silane coupling agent and a bonding agent were applied; cotton pellets were used as filling material and P-60 resin composite as restoring material. The cement-retained restorations were cemented with Rely-X U100. A metal rod with a spherical tip of 6.0 mm diameter was used to apply a vertical static load, simultaneously on the buccal and lingual incline cusps, at a crosshead speed of 0.5 mm/min until the fracture of the specimens. Data were analyzed using one-way ANOVA and Dunnet test (p<0.05) for multiples comparisons. The mode of failure was evaluated by a scanning electron microscopy (SEM). No significant difference between screw-retained restorations was found. The highest mean fracture resistance values were observed with CR group. Therefore, it was shown that SAH sealing did not influence the fracture resistance of the screw-retained restorations.

Resumo O objetivo deste estudo foi avaliar a influência do selamento do orifício de acesso ao parafuso (OAP) na resistência à fratura de coroas metalocerâmicas implantossuportadas aparafusadas. Pilares UCLA foram utilizados para fazer 30 molares inferiores implantossuportados e divididos igualmente em três grupos: Grupo AS: coroas aparafusadas com o OAP selado; Grupo ANS: coroas aparafusadas com o OAP não selado; Grupo CC: coroas cimentadas. O seguinte protocolo foi utilizado para selar o OAP: a superfície de cerâmica do OAP foi jateada com óxido de alumínio; condicionada com ácido fluorídrico a 10%; um agente silano e um adesivo dentinário foram aplicados; bolinhas de algodão foram utilizadas como material de preenchimento e a resina composta P-60 como material de restauração. As coroas cimentadas foram cimentadas com Rely-X U100. Uma haste de metal com uma ponta esférica de 6.0 mm de diâmetro foi usada para aplicar uma carga estática vertical, simultaneamente nas vertentes triturantes das cúspides linguais e vestibulares, com 0,5 mm/min velocidade do atuador até a fratura das coroas. Os dados foram analisados através de ANOVA um fator e teste de Dunnett (p<0,05) para as comparações múltiplas. O modo de falha foi avaliado por um microscópio eletrônico de varredura (MEV). Não houve diferença significativa entre restaurações aparafusadas. Os maiores valores médios de resistência à fratura foram observados com o Grupo CC. Portanto, observou-se que o selamento do OAP não influenciou a resistência à fratura das coroas aparafusadas.