Mining novel cis-regulatory elements from the emergent host Rhodosporidium toruloides using transcriptomic data.

The demand for robust microbial cell factories that produce valuable biomaterials while resisting stresses imposed by current bioprocesses is rapidly growing. Rhodosporidium toruloides is an emerging host that presents desirable features for bioproduction, since it can grow in a wide range of substrates and tolerate a variety of toxic compounds. To explore R. toruloides suitability for application as a cell factory in biorefineries, we sought to understand the transcriptional responses of this yeast when growing under experimental settings that simulated those used in biofuels-related industries. Thus, we performed RNA sequencing of the oleaginous, carotenogenic yeast in different contexts. The first ones were stress-related: two conditions of high temperature (37 and 42°C) and two ethanol concentrations (2 and 4%), while the other used the inexpensive and abundant sugarcane juice as substrate. Differential expression and functional analysis were implemented using transcriptomic data to select differentially expressed genes and enriched pathways from each set-up. A reproducible bioinformatics workflow was developed for mining new regulatory elements. We then predicted, for the first time in this yeast, binding motifs for several transcription factors, including HAC1, ARG80, RPN4, ADR1, and DAL81. Most putative transcription factors uncovered here were involved in stress responses and found in the yeast genome. Our method for motif discovery provides a new realm of possibilities in studying gene regulatory networks, not only for the emerging host R. toruloides, but for other organisms of biotechnological importance.

Análise dos perigos microbiológicos em amostras de caldo de cana / Analysis of microbiological hazards in samples of sugarcane juice

O caldo de cana é uma bebida nutritiva, muito comercializada por vendedores formais e ambulantes que, muitas vezes, não possuem condições adequadas de preparo, armazenamento e manipulação do caldo. O objetivo deste trabalho foi avaliar a contaminação microbiológica do caldo de cana comercializado por vendedores formais e ambulantes na cidade de Uberlândia/MG. Foram analisadas dez amostras do produto para contagem total de bactérias mesófilas, NMP/mL de coliformes totais e termotolerantes, pesquisa de Escherichia coli (e outras bactérias entéricas), incluindo Salmonella spp. Coliformes totais foram recuperados em todas as amostras e os termotolerantes em 70% delas, apresentando valores acima do padrão estabelecido pela legislação vigente. Não foram encontradas E. coli ou Salmonella spp., porém outras bactérias entéricas foram recuperadas em todas as amostras, sendo a mais prevalente Klebsiella pneumoniae. Os resultados mostram a necessidade de melhorias durante todos os processos realizados para obtenção do caldo de cana, a fim de minimizar a contaminação e presença de bactérias patogênicas.(AU)

Sugarcane juice is a nourishing drink marketed by formal and street vendors, who often lack adequate brewing preparation, storage, and handling conditions. This work aimed to evaluate the microbiological contamination of cane juice marketed by formal and ambulant vendors in Uberlandia/MG. Ten product samples have been analyzed for a total count of mesophilic bacteria, NMP/mL of total and thermotolerant coliforms, Escherichia coli (and other enteric bacteria) and Salmonella spp. Total coliforms were recovered in all samples and thermotolerant in 70% of the total, presenting values above the standard established by the current legislation. Not found Escherichia coli or Salmonella spp., but other enteric bacteria have been recovered in all samples, the most prevalent being Klebsiella pneumoniae. The results show the need for improvements during all processes performed to obtain the sugarcane juice to minimize the contamination and the presence of pathogenic bacteria.(AU)

Removal of phenolic, turbidity and color in sugarcane juice by electrocoagulation as a sulfur-free process.

This work analyzed the use of electrocoagulation as substitute for sugarcane clarification process using sulfitation. It was evaluated technological parameters (Icumsa color and turbidity), phenolic compounds content and CIELAB color parameters. Four kinetics of reduction color from sugarcane juice were carried out. The essays were divided according to the voltage applied: 35, 45, 55 and 65 V (also based on previous tests). Higher voltage treatments achieved greater reduction of Icumsa color, turbidity and total phenolic compounds. However, none of treatments impacted simple phenolic content analyzed in this work. Tristimulus analysis presented some pattern that went beyond technological analysis, including that 65 V essay changed the pigmentation of sugarcane juice and had an early stabilization on chroma. This kind of results could be useful for industry, once they could correlate quality with different color parameters and finally improve the clarification in general with finer settings of technique according to different situations.

Determination of nutrients in sugarcane juice using slurry sampling and detection by ICP OES.

The fractional factorial and Doehlert designs for optimization of a slurry sampling procedure to determine of nutrients in sugarcane juice by inductively coupled plasma optical emission spectrometry (ICP OES) were applied. External calibration curves were used for direct analysis of the slurry. This procedure allowed determination of Ca, Cu, Fe, K and Mg with limits of detection (LoD) obtained of 2.0, 0.04, 0.2, 1.0 and 1.5 mg L-1, respectively. The precision was expressed as relative standard deviation (%RSD), being better than 1.4% (n = 3). Accuracy was confirmed by comparison with sample digestion method. The results for analysis of fourteen sugarcane juices samples demonstrated that the nutrients Ca, Cu, Fe, K and Mg have average contents of 108, 0.506, 6.40, 470 and 114 mg L-1, respectively. The proposed analytical method is a good alternative for simultaneous determination of nutrients in sugarcane juice using introduction of slurries and detection by ICP OES.

Interaction of Saccharomyces cerevisiae-Lactobacillus fermentum-Dekkera bruxellensis and feedstock on fuel ethanol fermentation.

The alcoholic fermentation for fuel ethanol production in Brazil occurs in the presence of several microorganisms present with the starter strain of Saccharomyces cerevisiae in sugarcane musts. It is expected that a multitude of microbial interactions may exist and impact on the fermentation yield. The yeast Dekkera bruxellensis and the bacterium Lactobacillus fermentum are important and frequent contaminants of industrial processes, although reports on the effects of both microorganisms simultaneously in ethanolic fermentation are scarce. The aim of this work was to determine the effects and interactions of both contaminants on the ethanolic fermentation carried out by the industrial yeast S. cerevisiae PE-2 in two different feedstocks (sugarcane juice and molasses) by running multiple batch fermentations with the starter yeast in pure or co-cultures with D. bruxellensis and/or L. fermentum. The fermentations contaminated with D. bruxellensis or L. fermentum or both together resulted in a lower average yield of ethanol, but it was higher in molasses than that of sugarcane juice. The decrease in the CFU number of S. cerevisiae was verified only in co-cultures with both D. bruxellensis and L. fermentum concomitant with higher residual sucrose concentration, lower glycerol and organic acid production in spite of a high reduction in the medium pH in both feedstocks. The growth of D. bruxellensis was stimulated in the presence of L. fermentum resulting in a more pronounced effect on the fermentation parameters than the effects of contamination by each microorganism individually.

Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice

ABSTRACT The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice. The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design. The response surface was utilized to determine the levels that, among the variables that influence dextranase production, provide higher production of these enzymes. The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated. It was observed that only NaNO3 and pH showed significant effect (p<0.05) over dextranase production and was determined that the levels which provided higher enzyme production were, respectively, 5 g/L and 5.5. The dextranases produced by fungus P. chlamydosporia reduced by 75% the dextran content of the sugarcane juice once treated for 12 hours, when compared to the control treatment.

Molecular detection of Trypanosoma cruzi in acai pulp and sugarcane juice.

Chagas disease, caused by Trypanosoma cruzi affects about 6-8 million people worldwide. Although transmission by triatomine insects has been controlled, other means of transmission maintain the infection. These forms of transmission are responsible for introducing Chagas disease in other non-endemic countries of the world. Thus, Chagas disease, nowadays is a worldwide health problem. In Brazil, acai pulp and sugarcane juice have been associated with Chagas disease outbreaks. The difficulties in isolation of the parasite from foods are hampering source tracking which could allow the confirmation of an implicated food commodity in these outbreak investigations. To address this scientific gap, we evaluated the performance of real-time PCR (qPCR) for detecting T. cruzi in acai pulp and sugarcane juice. All experiments were performed with acai pulp and sugarcane juice samples contaminated with different concentrations of T. cruzi. In assays with qPCR, the results showed that the ideal procedure for T. cruzi identification in acai pulp and sugarcane juice consisted of: i. centrifugation; ii. DNA extraction with a commercial kit for stool matrix; and iii. qPCR using a specific molecular marker for T. cruzi. The seeding in LIT medium of experimentally contaminated foods was effective in detecting the parasitic load by qPCR. The efficacy of qPCR was also verified testing food samples crushed with infected Triatomines. In conclusion, this methodology can be used to perform rapid diagnosis in outbreaks, facilitating measures in disease control.

Molecular detection of Trypanosoma cruzi in acai pulp and sugarcane juice

Chagas disease, caused by Trypanosoma cruzi affects about 6-8 million people worldwide. Although transmission by triatomine insects has been controlled, other means of transmission maintain the infection. These forms of transmission are responsible for introducing Chagas disease in other non-endemic countries of the world. Thus, Chagas disease, nowadays is a worldwide health problem. In Brazil, acai pulp and sugarcane juice have been associated with Chagas disease outbreaks. The difficulties in isolation of the parasite from foods are hampering source tracking which could allow the confirmation of an implicated food commodity in these outbreak investigations. To address this scientific gap, we evaluated the performance of real-time PCR (qPCR) for detecting T. cruzi in acai pulp and sugarcane juice. All experiments were performed with acai pulp and sugarcane juice samples contaminated with different concentrations of T. cruzi. In assays with qPCR, the results showed that the ideal procedure for T. cruzi identification in acai pulp and sugarcane juice consisted of: i. centrifugation; ii. DNA extraction with a commercial kit for stool matrix; and iii. qPCR using a specific molecular marker for T. cruzi. The seeding in LIT medium of experimentally contaminated foods was effective in detecting the parasitic load by qPCR. The efficacy of qPCR was also verified testing food samples crushed with infected Triatomines. In conclusion, this methodology can be used to perform rapid diagnosis in outbreaks, facilitating measures in disease control.

Determination of pesticides in sugarcane juice employing microextraction by packed sorbent followed by gas chromatography and mass spectrometry.

This paper describes the development of a method for the determination of six pesticides (tebuthiuron, carbofuran, atrazine, metribuzine, ametryn, and bifenthrin) in sugarcane juice using microextraction by packed sorbent as the extraction technique. The extraction steps were optimized by factorial design, being the variables pH, ionic strength, desorption solvent and solvent volume optimized for comparisons among sorbent materials. Among the evaluated materials C18-Chromabond(®) showed better extraction efficiency. A factorial design 2(3) with central point was used for the extraction cycles optimization. Draw/eject and washes cycles showed significant improvements in the extraction efficiency when the number of cycles increased. The method was validated and showed a limit of quantification in the range of 2.0-10.0 µg.L(-1) . The calibration curves were constructed by weighting models that reduced the sum of absolute residues values and improved determination coefficient. The matrix factor and extraction efficiency were 97.3-77.3% and 27.1-64.8%, respectively. The accuracy was 71.7-106.9%; precision evaluated as the coefficient of variance obtained in intra and inter day analysis was 4.5-15.9%. The method was applied to the determination of pesticide residues in four sugarcane juice samples commercially available in markets from different cities from São Paulo state, Brazil.

Análise parasitológica e microbiológica de suco de laranja in natura e caldo de cana comercializados por ambulantes nas cidades de Crato e Juazeiro do Norte CE / Parasitological and microbiological analysis of orange juice fresh and sugarcane juice sold by street vendors in the cities of Crato and Juazeiro do Norte - CE

o suco de laranja e caldo de cana são bebidas muito apreciadas por suas características sensoriais e nutritivas. Estes produtos são preparados normalmente por ambulantes, em pontos de venda de comércio informal, que raramente tem treinamento profissional e desconhecem sobre normas de boas práticas de manipulação de alimentos. Considerando estes locais insalubres e com condições higienicossanitárias insatisfatórias foram realizadas análises bacteriológica, parasitológicas e micológicas em 30 amostras comercializadas nas cidades de Crato e Juazeiro do Norte - CE. As amostras foram semeadas em meio CPS para as análises bacteriológicas e micológicas e para análises parasitológicas foram realizadas as técnicas de Hoffman e de Faust. Nas análises bacteriológicas foi identificada a presença das bactérias Klebsiella spp., Escherichia coli, Staphylococcus spp. e Pseudomonas spp. Foi identificada também a presença de Candida spp. Todas as amostras foram negativas para as análises parasitológicas. Com I base nestes resultados, sugerem-se maiores cuidados nas boas práticas de fabricação, principalmente na higiene pessoal de manipuladores e sanificação de máquinas extratoras. (AU)

The orange juice and sugar cane juice drinks are highly appreciated for its organoleptic and nutritional characteristics. These products are usually prepared by street vendors in sales points of informal trade that has seldom unaware about professional training and practice standards of food handling. Considering these local unsanitary conditions and inadequate sanitary bacteriological analyzes were carried, mycological and parasitological 30 samples marketed in the cities of Crato and Juazeiro do Norte - CE. Samples were sown amid CPS for bacteriological and mycological analyzes and analyzes were made parasitological techniques Hoffman technique and Faust. In bacteriological analysis was identified the presence of the bacteria Klebsielia spp., Escherichia coli, Staphylococcus spp., and Pseudomonas spp. It was also identified the presence of Candida spp. Ali samples were negative for parasitological analysis. Based on these results, we suggest greater care in good manufacturing practices, particularly in personnel hygienic and sanitation machinery extractors. (AU)

Análise microbiológica e das condições higienicossanitárias do caldo de cana comercializado nas ruas do município de Cuiabá / Microbiological analysis and sanitary conditions of sugarcane juice sold in Cuiaba

A venda de alimentos por ambulantes é um fenômeno crescente no Brasil e, neste tipo de comércio, as condições inadequadas de manipulação e acondicionamento da matéria-prima podem trazer riscos à saúde da população. O presente trabalho objetivou verificar se o caldo de cana comercializado nas ruas do município de Cuiabá-MT atendia aos padrões microbiológicos preconizados pela legislação nacional vigente, para a determinação de coliformes termotolerantes e Salmonella spp, além de observar os cuidados higiênicos e sanitários do local de processamento e comercialização. Das 20 amostras de caldo de cana obtidas em 10 pontos da cidade, 75% apresentaram índices de contagens para coliformes a 45°C acima do padrão máximo aceitável de 102 NMP/mL, no entanto, em nenhuma das amostras foi detectada a presença de Salmonella. Das 15 amostras positivas para coliformes a 4SOC, 53,3% continham gelo, e 46,7% eram representadas por aquelas sem adição de gelo. Os pontos de venda pesquisados apresentavam más condições gerais, retratando o despreparo dos manipuladores e das condições das matérias-primas por eles processadas. Os dados obtidos nesta pesquisa permitem afirmar que o caldo de cana comercializado pelos ambulantes estava em condições higienicossanitárias insatisfatórias, por apresentarem índices elevados de contaminação por coliformes a 45°C. (AU)

The sale of food by street vendors is a growing phenomenon in Brazil, this trade the inadequate handling and packaging of raw materials can pose risks to public health. This study aimed to determine if the juice sold in the streets of the city of Cuiabá-MT, meets the microbiological standards recommended by national legislation inforce, with the determination of thermotolerant coliforms or 45ºC and Salmonella sp.. Besides observing the hygienic and sanitary processing site. Of the 20 samples of sugarcane juice valued at 10 points in the city, 75% had counts of coliforms at 45°C above the standard maximum acceptable 1()2 MPN /mL, however, in none of the samples showed the presence of Salmonella spp.. Of the 15 positive for coliforms at 45°C, 53, 3% contained ice, and 46, 7% were represented by those without this addition. Were observed in retail outlets surveyed general poor condition, portraying the unpreparedness of the handlers, and the conditions of raw materials processed by them since the time of receipt, storage and preparation of the product. The data obtained in this study have revealed that the juice was sold by street vendors inadequate sanitary conditions, therefore, had high levels of contamination by coliforms at 45 ºC. (AU)

Processamento e estabilidade de caldo de cana acidificado / Processing and stability of acidified sugarcane juice

O caldo de cana adicionado de polpa ou suco de fruta é uma bebida bastante apreciada no Brasil. O presente trabalho consistiu na elaboração, no processamento em escala piloto e no estudo da vida de prateleira de caldo de cana com polpa de maracujá. Processaram-se nove lotes de caldo de cana adicionado de 4% (m/m) de polpa de maracujá, a 85, 90 e 95 °C/30 s, em triplicata. A bebida pasteurizada foi envasada assepticamente em garrafas de polietileno tereftalato (PET) e estocada a 7 °C, na ausência de luz. Testes físico-químicos foram empregados na caracterização da bebida. Procedeu-se à análise do teor de vitamina C no caldo in natura e na bebida processada. Determinaram-se as atividades das enzimas polifenoloxidase (PPO) e peroxidase (POD) antes e após o processamento. A estimativa da vida útil do produto foi fundamentada em testes microbiológicos e sensoriais. A avaliação da estabilidade microbiológica baseou-se na enumeração de bactérias e fungos psicrotróficos. Testes de escala hedônica de sete pontos para avaliação da aparência, aroma, sabor e aceitação global foram empregados na análise da estabilidade sensorial. As médias dos valores de pH, sólidos solúveis e acidez titulável variaram entre 3,9 e 4,0; 17 e 23 °Brix; e 0,15 e 0,19% de ácido cítrico, respectivamente. A polpa de maracujá não incrementou o teor de ácido ascórbico da bebida que ficou entre 2,6 a 2,8 mg/ 100 mL de bebida. A atividade da PPO variou entre 3,1 e 40,3 U/mL para os nove lotes do caldo acidificado, antes da pasteurização. Os três binômios empregados no processamento foram efetivos na inativação da enzima. A atividade da POD oscilou entre 69,8 e 220,4 U/mL; contudo, apenas o processamento a 95 °C atingiu 100% de inativação. As contagens de bactérias e fungos em todos os nove lotes processados foram inferiores a 2 log UFC/mL ao longo de 90 dias de estocagem. A bebida atingiu médias de notas entre 5 e 6 para todos os atributos avaliados após 30 dias de estocagem. As médias dos tempos de vida útil da bebida processada a 85, 90 e 95 °C/30 s atingiram 31, 39 e 52 dias, respectivamente. Concluiu-se que o aumento da temperatura de pasteurização afetou positivamente a estabilidade da bebida. Contudo, o decréscimo da aceitação sensorial limitou a sua vida útil, no qual o sabor foi o principal atributo limitante.

Sugarcane juice, either pure or with fruit pulp addition, is an appreciated beverage in Brazil. The aim of this research was to evaluate the shelf-life of sugarcane juice mixed with passion fruit pulp (4% w/w). Nine batches were processed at 85, 90 and 95 °C/30 s, in three replicates. The pasteurized beverage was aseptically filled into polyethylene terephthalate (PET) bottles and stored at 7 °C without light exposure. pH, soluble solids and titrable acidity were measured during storage. The content of vitamin C was analyzed both in fresh sugar cane juice and pasteurized beverage. Polyphenoloxidase (PPO) and peroxidase (POD) activities were determined both before and after processing. Microbiological stability evaluation was based on psychrotrophic bacteria and fungi counts. Sensory acceptance was estimated by assigning a liking score on a 7-point hedonic scale to the attributes appearance, flavor, taste and overall appreciation. The mean values for pH, soluble solids and titratable acidity ranged from 3.8 to 4.3, 15 to 24 °Brix, and 0.13 to 0.17% of citric acid, respectively, for all processed batches. The passion fruit pulp did not increase the ascorbic acid content in the pasteurized product which ranged from 2.6 to 2.8 mg / 100 mL of beverage. The PPO activity varied from 3.1 to 40.3 U/mL for the nine acidified sugarcane juice batches before pasteurization. The three pasteurization binomials were effective for enzyme inactivation. The POD activity ranged from 69.8 to 220.4 U/mL. However, only processing at 95 °C achieved complete inactivation. The bacteria and fungi counts in all nine pasteurized batches were lower than 2 log CFU/mL up to 90 days of storage. The product achieved scores between 5 and 6 for all sensory attributes evaluated after 30 days of storage. The estimated shelf-life for beverage processed at 85, 90 and 95 °C/30 s was 31, 39 and 52 days, respectively. It was concluded that pasteurization temperature had a positive effect on beverage stability. Nevertheless, the decrease in flavor acceptance was the main limiting factor for the product/'s shelf-life.

Produtividade de rapadura de genótipos de cana-de-açúcar na Região de Dueré, sul de Tocantins / Scraping productivity of sugarcane genotypes at the south of Tocantins state

Avaliou-se o comportamento de treze genótipos de cana de açúcar quanto à produção de rapadura, na região de Dueré, sul do estado de Tocantins. A pesquisa foi conduzida no assentamento Santa Tereza, município de Dueré-TO. O delineamento experimental utilizado foi o de blocos ao acaso com três repetições. Os genótipos de cana avaliados foram SP80-1816, SP81-3250, RB56-5230, RB92-8064, SP80-1842, SP85-3877, RB86-7515, RB83-5486, SP84-5210, IAC86-2480, SP86-155, RB85-5536 e Nativa-25. As características avaliadas foram altura das plantas, massa do colmo industrial, massa dos ponteiros, sólidos solúveis, quantidade de garapa, produtividade de rapadura, relação colmo industrial/garapa, relação garapa/rapadura, relação colmo industrial/rapadura, e porcentagem de floração. O genótipo nativa-25 não é recomendado para produção de rapadura; o genótipo RB92-8064 é recomendado para produção de rapadura e apresenta potencial para ser utilizado na alimentação animal.

The scraping production of thirteen sugarcane genotypes was evaluated at the South of Tocantins State. The research was carried out at the Santa Tereza settlement, located at the municipality of Dueré, Tocantins State. The experiment was carried out in a completely randomized blocks design with three replicates. The sugarcane genotypes evaluated were SP80-1816, SP81-3250, RB56-5230, RB92-8064, SP80-1842, SP85-3877, RB86-7515, RB83-5486, SP84-5210, IAC86-2480, SP86-155, RB85-5536 and Nativa-25. The evaluated characters were plant height, mass of industrial stalk, mass of pointers, soluble solids, yielding of sugarcane juice, scraping productivity, ration industrial stalk/ sugarcane juice, ratio of sugarcane juice/scraping, ratio of industrial stalk/ scraping and blossom percentage. According to the results obtained, the genotype Nativa-25 is not recommended to scraping production, while the genotype RB92-8064 can be recommended to scraping production and it showed the highest potential to be used as animal food.

Scraping productivity of sugarcane genotypes at the South of Tocantins State / Produtividade de rapadura de genótipos de cana-de-açúcar na Região de Dueré, Sul de Tocantins

The scraping production of thirteen sugarcane genotypes was evaluated at the South of Tocantins State. The research was carried out at the Santa Tereza settlement, located at the municipality of Dueré, Tocantins State. The experiment was carried out in a completely randomized blocks design with three replicates. The sugarcane genotypes evaluated were SP80-1816, SP81-3250, RB56-5230, RB92-8064, SP80-1842, SP85-3877, RB86-7515, RB83-5486, SP84-5210, IAC86-2480, SP86-155, RB85-5536 and Nativa-25. The evaluated characters were plant height, mass of industrial stalk, mass of pointers, soluble solids, yielding of sugarcane juice, scraping productivity, ration industrial stalk/ sugarcane juice, ratio of sugarcane juice/scraping, ratio of industrial stalk/ scraping and blossom percentage. According to the results obtained, the genotype Nativa-25 is not recommended to scraping production, while the genotype RB92-8064 can be recommended to scraping production and it showed the highest potential to be used as animal food. 

Avaliou-se o comportamento de treze genótipos de cana de açúcar quanto à produção de rapadura, na região de Dueré, sul do estado de Tocantins. A pesquisa foi conduzida no assentamento Santa Tereza, município de Dueré-TO. O delineamento experimental utilizado foi o de blocos ao acaso com três repetições. Os genótipos de cana avaliados foram -1816, SP81-3250, RB56-5230, RB92-8064, SP80-1842, SP85-3877, RB86-7515, RB83-5486, SP84-5210, IAC86-2480, SP86-155, RB85-5536 E Nativa-25. As características avaliadas foram altura das plantas, massa do colmo industrial, massa dos ponteiros, sólidos solúveis, quantidade de garapa, produtividade de rapadura, relação colmo industrial/garapa, relação garapa/rapadura, relação colmo industrial/rapadura, e porcentagem de floração. O genótipo nativa-25 não é recomendado para produção de rapadura; o genótipo RB92-8064 é recomendado para produção de rapadura e apresenta potencial para ser utilizado na alimentação animal.