Sex-dependent behavioral effects of chronic nicotine during adolescence evaluated in young adult rats tested in Hole-Board.

As one of the leading causes of death and serious illnesses, tobacco smoking remains a significant issue in modern societies. Many individuals smoke during adolescence, a trend that has been exacerbated by the prevalence of vaping among young people. In this context, studying the behavioral effects induced by nicotine administration in male and female rats, during the adolescent period, assumes great importance because it can help to better understand the dynamics underlying tobacco use in the two sexes. For this purpose, we employed 4 groups of rats, 2 male and 2 female groups, chronically treated with saline or nicotine 3 mg/kg i.p. for 30 days, spanning from postnatal day 30 to postnatal day 60. Utilizing quantitative analyses and T-pattern detection and analysis, our findings revealed a complex and multifaceted behavioral reorganization in adolescent rats subjected to chronic nicotine administration. Specifically, we observed an increase of anxiety in males and a reduction in females. The distinctive structural changes, induced by chronic nicotine in both sexes, have significant implications, from a translational perspective, for studies on nicotine dependence disorders.

A quantitative and T-pattern analysis of anxiety-like behavior in male GAERS, NEC, and Wistar rats bred under the same conditions, against a commercially available Wistar control group in the hole board and elevated plus maze tests.

AIM: The Genetic Absence Epilepsy Rats from Strasbourg (GAERS) are an inbred polygenic model of childhood absence epilepsy (CAE), which, as their non-epileptic control (NEC) rats, are derived from Wistar rats. While the validity of GAERS in reproducing absence seizures is well established, its use as a model for CAE psychiatric comorbidities has been subject to conflicting findings. Differences in colonies, experimental procedures, and the use of diverse controls from different breeders may account for these disparities. Therefore, in this study, we compared GAERS, NEC, and Wistar bred in the same animal facility with commercially available Wistar (Cm Wistar) as a third control. METHODS: We performed hole board (HB) and elevated plus maze (EPM) tests that were analyzed with standard quantitative and T-pattern analysis in male, age-matched Cm Wistar and GAERS, NEC, and Wistar, bred under the same conditions, to rule out the influence of different housing factors and provide extra information on the structure of anxiety-like behavior of GAERS rats. RESULTS: Quantitative analysis showed that GAERS and NEC had similar low anxiety-like behavior when compared to Cm Wistar but not to Wistar rats, although a higher hole-focused exploration was revealed in NEC. T-pattern analysis showed that GAERS, NEC, and Wistar had a similar anxiety status, whereas GAERS and NEC exhibited major differences with Cm Wistar but not Wistar rats. EPM results indicated that GAERS and NEC also have similar low anxiety compared to Cm Wistar and/or Wistar rats. Nevertheless, the analysis of the T-pattern containing open-arm entry showed GAERS and Wistar to be less anxious than NEC and Cm Wistar rats. CONCLUSION: To summarize, comorbid anxiety may not be present in male GAERS rats. This study also highlighted the importance of including a control Wistar group bred under the same conditions when evaluating their behavior, as using Wistar rats from commercial breeders can lead to misleading results.

The hole-board apparatus in the study of anxiety.

Anxiety disorders pose a significant challenge in contemporary society, and their impact in terms of social and economic burden is overwhelming. Behavioral research conducted on animal subjects is crucial for comprehending these disorders and, from a translational standpoint, for introducing innovative therapeutic approaches. In this context, the Hole-Board apparatus has emerged as a widely utilized test for studying anxiety-related behaviors in rodents. Although a substantial body of literature underscores the utility and reliability of the Hole-Board in anxiety research, recent decades have witnessed a range of studies that have led to uncertainties and misinterpretations regarding the validity of this behavioral assay. The objective of this review is twofold: firstly, to underscore the utility and reliability of the Hole-Board assay, and concurrently, to examine the underlying factors contributing to potential misconceptions surrounding its utilization in the study of anxiety and anxiety-related behaviors. We will present results from both conventional quantitative analyses and multivariate approaches, while referencing a comprehensive collection of studies conducted using the Hole-Board.

Sudden bio-mathematical self-similarity and the uniqueness of human mass societies: from T-patterns and T-strings to T-societies.

With the explosive growth of human knowledge especially in the twenteeth century with even greater facilitation of access to knowledge, the world of even relatively recent great thinkers becomes daunting as seen from a modern viewpoint. Recently, humans ignored the existence of the complex intracellular world of cell organs, giant information molecules such as DNA, societies of specialized worker molecules (proteins), and generally the surprising nanoscale world visible to humanity since only a few decades ago. Moreover, computational power and video technology were inaccessible to all scientists from, for example, Aristotle to Freud, so new views and ideas seem to be expected about phenomena at all scales including nano and human. Some have arrived very recently. Thus urgently needed knowledge about the biology of animal and human behavior received the first Nobel Prize as late as 1973, in Physiology and Medicine, shared by Karl von Frisch, Konrad Lorenz, and Niko Tinbergen. Lorenz's Nobel lecture was entitled "Analogy as a Source of Knowledge" which did not mention self-analogy (self-similarity) as none of the species studied were part of others and knowledge of the nanoscale phenomena at the heart of this article had barely become available. The views and empirical findings presented in this article depend on such recent intracellular nanoscale insights and the development of a set of mathematical patterns, called T-system, of which only two are considered, the self-similar (i.e., parts having a structure similar to the whole) T-pattern and the derived T-string, a T-patterned material string (here, polymer or text). Specially developed algorithms implemented in the THEMETM software for T-pattern detection and analysis (TPA) allowed the detection of interaction T-patterns in humans, animals, and brain neuronal networks, showing self-similarity between animal interaction patterns and neuronal interaction patterns in their brains. TPA of DNA and text also showed unique self-similarity between modern human literate mass societies and the protein societies of their body cells, both with Giant Extra-Individual Purely Informational T-strings (GEIPIT; genomes or textomes) defining the behavioral potentials of their specialized citizens. This kind of society is here called T-society and only exists in humans and proteins, while the self-similarity between them only exists in human T-societies.

New insights into the behavioral structure of Pikler educators: An application of T-pattern detection and analysis with THEME.

Introduction: The recent generalization of early childhood schooling has given rise to a need for the development of rigorous, specific training programs aimed at early education professionals (0-3 years of age). This work emanates from the unique nature of Pikler-Lóczy education as a reference for early childhood education centers, and its expertise in providing quality care in an everyday classroom situation. The aim of the study is to use T-pattern detection and analysis, within the framework of observational methodology, to identify relevant aspects of the choreography followed by Pikler educators during breakfast, and thus provide substantial material with a view to creating a training plan. Methods: Two expert educators in Pikler-Lóczy education were directly and systematically observed in their own classrooms, following a nomothetic, follow-up and multidimensional design. The observation instrument underwent a molarization process so that the detected elements would be more significant and understandable for novice educators. Results: Using THEME, the invariant aspects of the educators' behavior were analyzed. The significance level for the critical interval was the THEME default (α = 0.005). The results show: on the one hand, changes expected in T-patterns in accordance with the observation instrument molarity; and on the other hand, stability in terms of the comparison between the two educators. Discussion: These results help us to identify the complex structure of the breakfast choreography, and how educators establish interaction with children. In addition to specific issues pertaining to Pikler-Lóczy education, the conclusions highlight the advantages of using T-pattern analysis within the framework of observational methodology, in order to dissect a mealtime routine in its natural context, and explain components of early childhood education intervention that satisfy basic childhood needs. This constitutes a starting point from which to generate instructive material for the training of professionals working in early education.

T-pattern detection in the scientific literature of this century: A systematic review.

Introduction: Scientific literature contains mainly systematic reviews focused on substantial aspects, but there are also approaches that have combined both substantial and methodological aspects, which is our preferred option since it undeniably adds value. The aims of this study were: (1) to carry out a systematic review of the literatura on T-Pattern analysis (TPA), and (2) to explore the possible contribution of mixed methods research to the integration of qualitative and quantitative elements on a synthesis level. Methods: Based on PRISMA guidelines, searches were carried out in the Scopus, PsycINFO, and Web of Science databases. The general search syntax was: "THEME" AND ("T-Patterns" OR "T Patterns") carried out in title, keywords and abstract. In addition, we included empirical articles on THEME and T-Patterns collected in other sources based on citations in several empirical works and consultations with different authors. This selection process resulted in 125 primary documents making up this systematic review. Results: The results showed that the detection of structures in behavior patterns forms a nexus between studies carried out in very diverse fields and contexts. Most studies are observational, whilst the applicability and power of T-Pattern detection are extraordinary. It allows the researcher to go deeper in a robust analysis that responds to the integration of qualitative and quantitative elements which constitutes the leit motive of mixed methods; and also to discover the deep, hidden structure that underlies the respective databases, regardless of the methodology used in each study. The possibilities in assigning parameters notably increase the options for obtaining results and their interpretation. Discussion: It is relevant the extraordinary strength and applicability of T-pattern detection. There is a high presence of T-pattern detection and analysis in studies using observational methodology. It is necessary commit to consolidating the methodological analysis of selected works, as taking individual and collective responsibility for improving methodological quality of TPA studies, taking advantage of the resources provided by the THEME program.

Structural analyses in the study of behavior: From rodents to non-human primates.

The term "structure" indicates a set of components that, in relation to each other, shape an organic complex. Such a complex takes on essential connotations of functionally unitary entity resulting from the mutual relationships of its constituent elements. In a broader sense, we can use the word "structure" to define the set of relationships among the elements of an emergent system that is not determined by the mere algebraic sum of these elements, but by the interdependence relationships of these components from which the function of the entire structure itself derives. The behavior of an integrated living being can be described in structural terms via an ethogram, defined as an itemized list of behavioral units. Akin to an architectural structure, a behavioral structure arises from the reciprocal relationships that the individual units of behavior establish. Like an architectural structure, the function of the resulting behaving complex emerges from the relationships of the parts. Hence, studying behavior in its wholeness necessitates not only the identification of its constitutive units in their autarchic individuality, but also, and importantly, some understanding of their relationships. This paper aimed to critically review different methods to study behavior in structural terms. First, we emphasized the utilization of T-pattern analysis, i.e., one of the most effective and reliable tools to provide structural information on behavior. Second, we discussed the application of other methodological approaches that are based on the analysis of transition matrices, such as hierarchical clustering, stochastic analyses, and adjusted residuals. Unlike T-pattern analysis, these methods allow researchers to explore behavioral structure beyond its temporal characteristics and through other relational constraints. After an overview of how these methods are used in the study of animal behavior, from rodents to non-human primates, we discussed the specificities, advantages and challenges of each approach. This paper could represent a useful background for all scientists who intend to study behavior both quantitatively and structurally, that is in terms of the reciprocal relationships that the various units of a given behavioral repertoire normally weave together.

Patterns of verbal interaction in newly formed music ensembles.

Ensemble rehearsal in the European classical music tradition has a relatively homogenised format in which play-through, discussion, and practice of excerpts are employed to establish and agree on performance parameters of notated music. This research analyses patterns in such verbal communication during rehearsals and their development over time. Analysing two newly established ensembles that work over several months to a performance, it investigates the interaction dynamics of two closely collaborating groups and adaptation depending on task demands, familiarity with each other and an upcoming deadline. A case study approach with two groups of five singers allowed in-depth exploration of individual behaviours and contributions; results are reported descriptively and supported by qualitative data. The results highlight changes over time that reflect the development of implicit (faster decisions) interactions from explicit (slower decisions). They show a trajectory of opening up and closing down in terms of interactional flexibility, enabling members to significantly contribute to the group, followed by tightening the interaction to establish stability for performance. These findings and novel employment of T-pattern analysis contribute to the understanding of human group behaviour and interaction patterns leading to expert team performance.

T-pattern analysis of offensive and defensive actions of youth football goalkeepers.

Nowadays, football goalkeepers (GKs) play an important role in the team's organization, namely, considering the offensive and defensive processes. The purpose of our investigation focuses on the notational and T-pattern analysis of the offensive and defensive actions of elite young football GKs. The participating GKs (n = 3, mean age of 16.6 years) presented 8 years of experience in the specific position, were internationally selected for the national team of Portugal, and competed in the national U-17 championship of Portugal. Thirty football matches were observed. The observational sample consisted of defensive actions (n = 225) and offensive actions (n = 296). Two observational instruments were used to codify the actions: the observation system of defensive technical-tactical actions of GKs and the observation system of offensive technical-tactical actions of GKs. Both instruments underwent a validation process, and inter- and intra-observer reliability was tested. The codification of the actions was performed with the LINCE program, and later the data were exported to Microsoft Excel and THEME 5.0. The notational data were analyzed in SPSS, and T-pattern detection analysis was performed in THEME 5.0. The predominant actions of young observed GKs were fundamentally goal defense and participation in the team's offensive process construction through actions performed with the foot and hand. The analysis of T-patterns allowed to identify T-pattern actions in the defensive actions of goal defense and exit of the goal, as well as related to the defensive set pieces. In the offensive process, the analysis of T-patterns reinforced the participation of the GK in the team's first phase of construction and in the execution of goal kicks and actions that start as a result of the actions of the opponent. The GK's defensive actions are mostly focused on the objective of goal defense and offensive actions with the hands and feet are important for their participation in positional attack construction. Our study contributes to a better knowledge of the GK's actions in the competition and is relevant to be considered by the specific position coaches in the training process organization.

A Systematic Review of the Use of T-Pattern and T-String Analysis (TPA) With Theme: An Analysis Using Mixed Methods and Data Mining Techniques.

In recent years, research interest in human and non-human behavioral analysis has increased significantly. One key element in the resulting studies is the use of software that facilitates comparative analysis of behavioral patterns, such as using T-Pattern and T-String analysis -TPA- with THEME. Furthermore, all these studies use mixed methods research. Results from these studies have indicated a certain amount of similarity between the biological, temporal, and spatial patterns of human social interactions and the interactions between the contents of their constituent cells. TPA has become an important, widely-used technique in applied behavioral science research. The objectives of the current review were: (1) To identify the results of research over the last 4 years related to the concepts of T-Pattern, TPA, and THEME, since it is in this period in which more publications on these topics have been detected (2) To examine the key concepts and areas in the selected articles with respect to those concepts, applying data and text mining techniques. The results indicate that, over the last 4 years, 20% of the studies were laboratory focused with non-humans, 18% were in sports environments, 9% were in psychological therapy environments and 9% were in natural human contexts. There were also indications that TPA is beginning to be used in workplace environments, which is a very promising setting for future research in this area.

Hierarchy and diffusion of organizational forms.

In this paper we first of all summarize and rationalize current typologies of organizational forms, arranging available classifications in a hierarchy of increasing generality. The ensuing structure parallels the classification of living beings into classes of increasing generality such as species, genus, family, order, and so on. Subsequently, we analyze the structure of communications that favored the diffusion of each organizational form. We isolate a few stylized communication structures, pointing to the presence of several sources endowed with global connections as the most efficient diffusion mode. The empirical research that is being carried out on single organizations is close to observing their T-patterns, whereas nothing comparable is in sight for organizational forms as yet. However, at least in some cases, we dare to formulate tentative hypotheses on certain features that the ensuing T-patterns-of-patterns might exhibit.

Análisis del error en los saltos xuanfengjiao y tengkong waibaitui en wushu. Un estudio piloto / Analysis of the Mistakes in Xuanfengjiao and Tengkong Waibaitui Jumps in Wushu

El objetivo de esta investigación es detectar y analizar mediante T-Patterns y coordenadas polares los errores técnicos y las secuencias conductuales que se producen en dos saltos con rotación de 540 grados integrados en las formas o rutinas de competición de wushu (taolu). Analizamos 47 formas de competición masculina sénior de ámbito internacional: 43 saltos Xuanfengjiao y 33 saltos Tengkong-Waibaitui. Los errores más frecuentes son el insuficiente grado de giro y la falta de altura de la patada a nivel de los hombros. Las secuencias de errores afectan al ataque del salto, al desarrollo aéreo, a la toma de suelo, y a la recepción y desequilibrios. Los técnicos, árbitros y entrenadores de wushu pueden utilizar estos resultados para mejorar el entrenamiento y desarrollo de la competición y perfeccionar la tarea de los docentes proporcionando feedbacks más precisos. (AU)

The objective of this research is to identify and analyze T-Patterns and polar coordinates and the technical and behavioral sequential mistakes that occur in two jumps with a rotation of 540 degrees integrated in the forms and routines of wushu competition (taolu). We analyzed 47 forms of an international senior men's competition: 43 Xuanfengjiao jumps and 33 Tengkong-Waibaitui jumps. The most frequent mistakes are the insufficient degree of rotation and the lack of height of the kick at shoulder level. The mistake sequences affect the attack of the jump, the aerial development, the landing on the floor, and the reception and imbalances. The wushu technicians, referees and coaches can use these results to improve the training and development of the competition and to improve the task of the teachers by providing more accurate feedbacks. (AU)

Effect of Teaching Method on Exercise Execution in Adolescents' Use of Outdoor Fitness Equipment.

The use of outdoor fitness equipment (OFE) is an effective strategy to promote physical activity. The equipment normally includes information panels with phrases and images describing appropriate exercises. However, as using this equipment inappropriately is a potential problem, it is important to find an optimal unsupervised instruction method for correct exercise execution. Our objective in this study was to determine which of several exercise prescription methods, without direct professional supervision, might best instruct adolescents to correctly engage in upper limb motor execution on outdoor flexion-extension equipment. A total of 54 adolescents from a middle socioeconomic level in northwest Spain participated in this descriptive and quasi-experimental study. We randomly assigned members of this convenience sample into three groups who received either video instruction, instruction via images or written panel instructions. We used observational methodology in videographic analysis to evaluate the mistakes participants made in motor execution with each instructional method. We utilized different analytic techniques from observational methodology: statistical analyses (means, standard deviation, confidence intervals, ANOVA, etc.) and detecting T-Patterns with Theme and polar coordinate analysis using HOISAN. Participants who relied on video instructions committed fewer errors than those who relied on panel instructions. The video method prevented loss of information that occurred when instructional images were used. We suggest including a QR code on outdoor fitness equipment in open-air parks to permit users to download an explanatory video to their mobile phones.

Inverted-T pattern reduction mammoplasty in bilateral breast ptosis: cosmetic and oncological outcomes.

BACKGROUND: Breast ptosis is directly caused by Cooper's ligament laxity, with the decline of nipple areola complex (NAC) and mammary parenchyma. Breast cancer with ptosis is always a knotty problem that can hardly be repaired by classic breast conservation surgery (BCS) ending up with a pleasing appearance. We analyzed our 12 years' experience of performing inverted-T pattern techniques to treat bilateral breast ptosis, with or without breast cancer. METHODS: One hundred forty-eight breasts in 74 patients undergoing inverted-T pattern reduction mammoplasty were included in this study. Information about patients' clinical and surgical characteristics, complications, NAC sensitivity, cosmetic and oncological outcomes were collected and retrospectively analyzed. RESULTS: In the cohort of 57 patients with pure breast ptosis, the mean body mass index (BMI) was 25.2 kg/m2, and the mean weight of resected tissue from the left and right breast reductions were 744.9 and 756.7 g. In the cohort of 17 patients diagnosed as breast cancer with ptosis, the mean BMI was 25.1 kg/m2, and the mean weight of resected tissue were 504.1 g for left and 535.6 g for right side. The majority of repairs were performed for tumors located in the upper outer (58.8%), mostly with inferior or superomedial pedicles (90%). All the upper inner tumors were repaired with inferior pedicles. Minor complications such as seroma (8.1%), NAC epidermolysis (8.1%), delayed wound healing (4.1%) were detected postoperatively. Partial NAC necrosis occurred in one patient (1.4%). 82.4% of all the patients rated "very satisfied" or "satisfied" as the final cosmetic outcomes. NAC sensitivity was "very high" and "high" in 82.4% patients. No local occurrence, distant metastasis and mortality occurred in tumor patients. CONCLUSIONS: The inverted-T pattern reduction mammoplasty is a reliable technique to treat bilateral breast ptosis with a low complication rate. For cases with breast cancer, this technique can achieve both satisfying cosmetic outcomes and oncological safety.

Effects of Different Anxiety Levels on the Behavioral Patternings Investigated through T-pattern Analysis in Wistar Rats Tested in the Hole-Board Apparatus.

The Hole-Board is an ethologically based tool for investigating the anxiety-related behavior of rats following manipulation of the central anxiety level. The present paper aims to assess behavioral patterning following pharmacological manipulation of emotional assets in Wistar rats tested in this experimental apparatus. For this purpose, the behavior of three groups of rats injected with saline, diazepam or FG7142 was evaluated using conventional quantitative and multivariate T-pattern analyses. The results demonstrate that quantitative analyses of individual components of the behavior, disjointed from the comprehensive behavioral structure, are of narrow utility in the understanding of the subject's emotional condition. Among the components of the behavioral repertoire in rodents tested in the Hole-Board, Edge-Sniff and Head-Dip represent the most significant ones to rate anxiety level. They are characterized by a strong bivariate relationship and are also firmly part of the behavioral architecture, as revealed by the T-pattern analysis (TPA), a multivariate technique able to detect significant relationships among behavioral events over time. Edge-Sniff → Head-Dip sequences, in particular, are greatly influenced by the level of anxiety: barely detectable in control animals, they completely disappear in subjects with a reduced level of anxiety and are present in almost 25% of the total of T-patterns detected in subjects whose anxiety level increased.

Detection of a temporal structure in the rat behavioural response to an aversive stimulation in the emotional object recognition (EOR) task.

Aim of the research was to investigate whether a temporal structure could be detected in the behavioural response to an aversive stimulation. A fear-related memory task was used in rats, placed in a modified version of the Novel Object Recognition task known as Emotional Object Recognition task, i.e. a behavioural assay that orbits around the declarative memory for an aversive experience. To this purpose, twelve male Wistar rats, divided in two groups (Control and Aversive memory), observed after 4 h (OR4h) and after 24 h (OR24h) from the delivery of an aversive stimulation, associated to a specific object, were used. Data were evaluated both in terms of conventional quantitative approaches and by means of T-pattern analysis, namely a multivariate technique able to unveil the temporal structure of behaviour and the relationships amongst the behavioural items in time. Results evidenced several changes between groups and over time as well. Mean occurrences and mean durations showed significant differences between OR4h and OR24h sessions and between Control and Aversive memory groups for behavioural items of Exploration, Object-related aversion and Immobility. T-pattern analysis revealed important changes of behavioural variability, complexity and repetitiveness, (i.e., the three main qualitative features of T-patterns) in the Aversive memory group. These outcomes highlight a simpler and linear behavioural profile, focused only on specific sequences of particularly repetitive events. Overall, the present study demonstrates a) the presence of a temporal organization of fear-related behavioural events and b) the influence of learning on the modifications observed over time.

Exploring playful asymmetries for gender-related decision-making through T-pattern analysis.

The focus of this study was on exploring decision-making in a triadic motor game (Bears, Monkeys and Ants), using T-Pattern analysis (TPA). The research involved 23 players aged 12 to 13 years (mean ± standard deviation; 12.5 ± 1), 12 girls and 11 boys, from a secondary middle-class school in Tenerife (Spain). An observational design was used (quadrant III): Nomothetic (a plurality of players), Punctual (a session recorded) and Multidimensional (different criteria). A mixed methods approach was used. It consisted of systematic observation to assess specific roles and subroles. A viewing instrument was built to observe the players 'ad hoc' while they were playing. To address the quality of the records, the inter-observer and intra-observer reliability and validity were calculated. We carried out a descriptive analysis of the behaviors' frequencies coded through Lince. For data analysis we use the tools IBM SPSS 25 and THEME v.6. TPA were performed according to collective (team by team), team-gender and individual. Although analyzes were performed (Chi-square (χ2)), comparing the participation between girls and boys in each team; no significant differences were found (p>.05). Greater complexity was pointed out throught T-Pattern analysis in Monkeys and Ants teams than in the Bears team. Girls showed greater variability than boys in Monkey and Ants teams. When girls used 3 different roles, boys only used 2 of them, being the conduct to realease peers (p <0.005) recurrent in Monkeys. The boys as a team (p <0.005) used subroles that directly modified the outcome in the Ants team. Similar T-Patterns were found in girls (p<.05) when analyzed individually. The use of TPA allows the detection of hidden features while girls and boys were playing. The apparent neutrality of the game may have a seemingly random decision-making process but TPA revealed specificities highly applicable to the study of gender through triadic motor games.

Brain histamine and oleoylethanolamide restore behavioral deficits induced by chronic social defeat stress in mice.

The physiological mechanisms underlying the complex interplay between life stressors and metabolic factors is receiving growing interest and is being analyzed as one of the many factors contributing to depressive illness. The brain histaminergic system modulates neuronal activity extensively and we demonstrated that its integrity is necessary for peripheral signals such as the bioactive lipid mediator oleoylethanolamide (OEA) to exert its central actions. Here, we investigated the role of brain histamine and its interaction with OEA in response to chronic social defeat stress (CSDS), a preclinical protocol widely used to study physio-pathological mechanisms underlying symptoms observed in depression. Both histidine decarboxylase null (HDC-/-) and HDC+/+ mice were subjected to CSDS for 21 days and treated with either OEA or vehicle daily, starting 10 days after CSDS initiation, until sacrifice. Undisturbed mice served as controls. To test the hypothesis of a histamine-OEA interplay on behavioral responses affected by chronic stress, tests encompassing the social, ethological and memory domains were used. CSDS caused cognitive and social behavior impairments in both genotypes, however, only stressed HDC+/+ mice responded to the beneficial effects of OEA. To detect subtle behavioral features, an advanced multivariate approach known as T-pattern analysis was used. It revealed unexpected differences of the organization of behavioral sequences during mice social interaction between the two genotypes. These data confirm the centrality of the neurotransmitter histamine as a modulator of complex behavioral responses and directly implicate OEA as a protective agent against social stress consequences in a histamine dependent fashion.

The impact of chronic daily nicotine exposure and its overnight withdrawal on the structure of anxiety-related behaviors in rats: Role of the lateral habenula.

Tobacco smoking is a serious health problem worldwide and a leading cause of mortality. Nicotine, the addictive component of tobacco, affects a range of emotional responses, including anxiety-related behaviors. Although perceived by smokers to be anxiolytic, evidence suggests that smoking increases anxiety and that mood fluctuates with nicotine intake. Thus, nicotine addiction may depend on easing the psychobiological distress caused by its abuse. The lateral habenula (LHb) has been implicated as a neural substrate for acute nicotine-induced anxiety, but its role in anxiety-like behaviors associated with chronic nicotine exposure has not been explored. Here, we assessed the effect of chronic nicotine exposure and its subsequent overnight withdrawal on anxiety-like behavior using both quantitative and multivariate T-pattern analysis in rats tested using the hole-board apparatus. Additionally, we explored the role of the LHb by comparing the behavioral effects of short-term nicotine withdrawal in chronically treated LHb-lesioned rats. Quantitative analysis revealed increased anxiety-like behavior in chronically treated overnight nicotine-deprived rats, as manifested in reduced general and focused exploratory behaviors, which was eased in animals that received nicotine. Quantitative analysis failed to reveal a role of the LHb in overnight nicotine deprivation-induced anxiety. Conversely, T-pattern analysis of behavioral outcomes revealed that chronic nicotine-treated rats still show anxiety-like behavior following nicotine challenge. Moreover, it demonstrated that the LHb lesion induced a stronger anxiolytic-like response to the acute challenge of nicotine in chronically nicotine-exposed animals, implicating the LHb in the anxiogenic effect of chronic nicotine exposure. These data further highlight the LHb as a promising target for smoking cessation therapies and support the importance of T-pattern analysis for behavioral analysis.

A Survey for the Ranking of Trajectory Prediction Algorithms on Ubiquitous Wireless Sensors.

The number of wireless sensors in use-for example, the global positioning system (GPS) intelligent sensor-has increased in recent years. These intelligent sensors generate a vast amount of spatiotemporal data, which can assist in finding patterns of movements. These movement patterns can be used to predict the future location of moving objects; for example, the movement of an emergency vehicle can be predicted for health care decision-making. Although there is a body of research work regarding motion trajectory prediction, there are no guidelines for choosing algorithms best suited for individual needs in uncertain and complex situations and as per the application domains. In this paper, we surveyed existing trajectory prediction algorithms. These algorithms are further ranked scientifically in terms of accuracy (performance), ease of use, and best fit as per the available datasets. Our results show three top algorithms, namely NextPlace, the Markov model, and the hidden Markov model. This study can be beneficial for multicriteria decision-making for various disciplines, including health care.