Hepatic heat shock proteins, antioxidant-related genes, and immunocompetence of heat-stressed broilers in response to short periods of incubation during egg storage and thermal conditioning.

Short Periods of Incubation During Egg Storage (SPIDES) approach improves chick quality and hatching rates. Also, embryonic thermal conditioning (TC) is a strategy for enhancing thermotolerance in avian species. Until now, evaluating the effect of either SPIDES or embryonic TC effects has only been separately conducted, so we hypothesized that combining TC and SPIDES may enhance the response of broilers to thermal stress. Eight hundred Ross broiler eggs were divided into two groups; the first one was kept under appropriate storage room conditions, S0 (control) The 2nd was subjected to SPIDES for 5 h at 37.8 ○C ± 0.1 three times at days 5, 10, and 15 (S1) after egg collection respectively. On the 14th day of incubation (DOI) each of the two main groups was randomly divided into two equal subgroups; the control one was left under the appropriate incubation settings (TC0) whereas the other received prenatal heat conditioning (TC1) at 39.5 ○C ± 0.1 for 6 h/d from the 14th to the 18th embryonic day (E), resulting finally in four experimental subgroups (S0TC0, S1TC0, S0TC1 & S1TC1). RESULTS: showed that SPIDES treatment improved the hatchability of the stored eggs by almost 20% compared to untreated eggs. A combination of SPIDES and TC (S1TC1) increased significantly the levels of Immunoglobulin (IgG and IgM) production at hatch and heat-stressed birds. Our findings revealed that the hepatic heat shock proteins (hsp70, 90 A,90 B, 60 and hspA9), antioxidants-related genes (CAT, and SOD2), and NADPH4 were significantly downregulated in the thermally conditioned group that challenged with thermal stress conditions. As opposed to that, the SPIDES group showed a significant increase in hepatic heat shock proteins, antioxidants-related genes, and NADPH4 when subjected to thermal-stress conditions. In conclusion, the combination of SPIDES and TC has a positive effect on some pre and post-hatch traits of broiler chicks. Under heat stress challenge, thermal conditioning can modify the expression of antioxidant-related genes and Hsps, leading to the enhanced acquisition of thermotolerance as evidenced by lower expression of Hsps and NADPH4. While SPIDES does not have a significant role in thermotolerance acquisition.

Electrothermaly conditioned carbon fibre for the analysis of volatile pollutants.

This study deals with the development of an inexpensive and single-step sorbent manufacturing methodology for the analysis of air pollutants. Disposable carbon fibre sorbents were prepared in a few minutes using the electrothermal conditioning technique. The sorbent conditioning current and time were optimised to obtain the best extraction of benzene, toluene, ethylbenzene and xylenes (BTEX) from the air samples. After sorbent characterisation, analysis parameters affecting the BTEX extraction efficiency, such as sampling volume, humidity and sampling flow rate, were optimised for active BTEX sampling. Under optimum conditions, validation parameters such as the limit of detection (LOD), repeatability, reproducibility, and linear range were found to be 0.07-0.11 mg m - 3, 1.1%-1.8%, 5.6%-9.5% and 0.24-45 mg m - 3, respectively. Thereafter, the BTEX analysis was successfully conducted using the proposed method, with acceptable recovery values (96%-103%) in the real indoor environments.

Growth performance and hepatic antioxidants responses to early thermal conditioning in broiler chickens.

There are little data about antioxidants' status responses to early thermal conditioning (TC) on broiler chickens. Therefore, the present study was conducted to investigate the different time ages of thermal conditioning on antioxidants responses and the growth rate of broiler chicks. A total of two hundred forty-one-day-old male broiler chicks (Cobb 500) weighed on average 51.5 ± 0.5g were randomly distributed into four equal groups (60 chicks each), and chicks of each group were ranked in five replicates. The first group reared under the ambient temperature, while the second, third and fourth groups (TC3, TC5 and TC7) were subjected to early-age thermal conditioning at 39°C for 6 h on the third, fifth and seventh day of age respectively. Broilers were fed ad-libitum, and drinking water was a free choice during the experimental period. At the end of the experimental period that lasted 5 weeks, all experimental groups were exposed to heat challenge at 36°C for 6 h. Early-age thermal conditioning did not affect growth performance. Plasma corticosterone elevation in TC5 (as a post-challenge response) was the lowest among the experimental groups. Hepatic malondialdehyde significantly increased in TC5 and TC7 groups both at the post-conditioning stage and at the end of the experimental period. Hepatic glutathione, glutathione S-transferases, catalase and superoxide dismutase significantly decreased by early-age thermal conditioning compared with non-conditioned broilers. Microscopic examination of the liver sections from broilers chickens in TC5 and TC7 groups showed all the basic features of normal liver tissue, while the control and TC3 groups showed few necrotic areas. It could be concluded that early-age thermal conditioning at 39°C for 6 h on the fifth day of age could improve the antioxidant defence system of broilers without any adverse effects on growth performance.

Thermal Endurance by a Hot-Spring-Dwelling Phylogenetic Relative of the Mesophilic Paracoccus.

High temperature growth/survival was revealed in a phylogenetic relative (SMMA_5) of the mesophilic Paracoccus isolated from the 78 to 85°C water of a Trans-Himalayan sulfur-borax spring. After 12 h at 50°C, or 45 min at 70°C, in mineral salts thiosulfate (MST) medium, SMMA_5 retained ~2% colony forming units (CFUs), whereas comparator Paracoccus had 1.5% and 0% CFU left at 50°C and 70°C, respectively. After 12 h at 50°C, the thermally conditioned sibling SMMA_5_TC exhibited an ~1.5 time increase in CFU count; after 45 min at 70°C, SMMA_5_TC had 7% of the initial CFU count. 1,000-times diluted Reasoner's 2A medium, and MST supplemented with lithium, boron, or glycine-betaine, supported higher CFU-retention/CFU-growth than MST. Furthermore, with or without lithium/boron/glycine-betaine, a higher percentage of cells always remained metabolically active, compared with what percentage formed single colonies. SMMA_5, compared with other Paracoccus, contained 335 unique genes: of these, 186 encoded hypothetical proteins, and 83 belonged to orthology groups, which again corresponded mostly to DNA replication/recombination/repair, transcription, secondary metabolism, and inorganic ion transport/metabolism. The SMMA_5 genome was relatively enriched in cell wall/membrane/envelope biogenesis, and amino acid metabolism. SMMA_5 and SMMA_5_TC mutually possessed 43 nucleotide polymorphisms, of which 18 were in protein-coding genes with 13 nonsynonymous and seven radical amino acid replacements. Such biochemical and biophysical mechanisms could be involved in thermal stress mitigation which streamline the cells' energy and resources toward system-maintenance and macromolecule-stabilization, thereby relinquishing cell-division for cell-viability. Thermal conditioning apparently helped inherit those potential metabolic states which are crucial for cell-system maintenance, while environmental solutes augmented the indigenous stability-conferring mechanisms. IMPORTANCE For a holistic understanding of microbial life's high-temperature adaptation, it is imperative to explore the biology of the phylogenetic relatives of mesophilic bacteria which get stochastically introduced to geographically and geologically diverse hot spring systems by local geodynamic forces. Here, in vitro endurance of high heat up to the extent of growth under special (habitat-inspired) conditions was discovered in a hot-spring-dwelling phylogenetic relative of the mesophilic Paracoccus species. Thermal conditioning, extreme oligotrophy, metabolic deceleration, presence of certain habitat-specific inorganic/organic solutes, and potential genomic specializations were found to be the major enablers of this conditional (acquired) thermophilicity. Feasibility of such phenomena across the taxonomic spectrum can well be paradigm changing for the established scopes of microbial adaptation to the physicochemical extremes. Applications of conditional thermophilicity in microbial process biotechnology may be far reaching and multifaceted.

Mapping of the Thermal Microenvironment for Dairy Cows in an Open Compost-Bedded Pack Barn System with Positive-Pressure Ventilation.

The objective of this study was to evaluate and characterize the dependence and the spatial and temporal distribution of variables and indices of the thermal environment in an open compost-bedded pack barn system with positive-pressure ventilation (CBPPV) during the winter period. The study was conducted in a CBPPV system located in the Zona da Mata region, Minas Gerais, Brazil. The indoor environment was divided into a mesh composed of 55 equidistant points, where data on dry-bulb air temperature (tdb) and relative humidity (RH) were collected. The collected data were divided into four periods-dawn, morning, afternoon, and night-and mean values were obtained. To evaluate the thermal microenvironment, the temperature and humidity index (THI) and the specific enthalpy of air (h) were used. For spatial dependence analysis, geostatistical techniques were applied. Through the results, a strong spatial dependence was verified for all variables evaluated. Through THI and h maps, conditions of thermal comfort were found for dairy cattle. The highest values of tdb, THI, and h were recorded in the afternoon period in the northwest region of the facility (tdb = 23.2 °C, THI = 69.7, and h = 50.9 kJ∙kg of dry air-1).

mRNA Expressions of Methylation Related Enzymes and Duration of Thermal Conditioning in Chicks.

DNA methylation regulates gene expression by modifying the nucleosome structure of DNA, without altering the gene sequence. It has been reported that DNA methylation reactions are catalyzed by several enzymes. In chickens, thermal conditioning treatment affects the central DNA methylation levels. The purpose of this study was to clarify the changes in DNA methylation and demethylation factors during thermal conditioning in the hypothalamus of 3-day-old chicks. Male chicks (3-days old) were exposed to 40±0.5°C as a thermal conditioning treatment for 1, 2, 6, 9, or 12 h. The control chicks were kept in a thermoneutral zone (30±0.2°C). After thermal conditioning, the mRNA levels of DNA methyltransferase (DNMT)-1, -3a, -3b, and ten-eleven translocation (TET)-1, -2, and -3 in the hypothalamus were measured by q-PCR. The mRNA levels of DNMT-3a and TET-1 were increased by thermal conditioning. Moreover, the expression level of TET-1 increased with the loading time of the thermal conditioning. The gene expressions of DNMT-1, DNMT-3b, TET-2, and TET-3 were not affected by thermal conditioning. Since DNMT-3a is a catalyst for de-novo DNA methylation and TET-1 catalyzes the oxidation of methylated cytosine, it is suggested that the thermal conditioning increased the activation of DNA methylation and demethylation factors, which occur in the hypothalamus of neonatal chicks.

Mitigating the detrimental effects of heat stress in poultry through thermal conditioning and nutritional manipulation.

The poultry industry faces several obstacles and challenges, including the changes in global temperature, increase in the per capita demand for meat and eggs, and the emergence and spread of various diseases. Among these, environmental challenges are one of the most severe hurdles impacting the growth and productivity of poultry. In particular, the increasing frequency and severity of heat waves over the past few years represent a major challenge, and this is expected to worsen in the coming decades. Chickens are highly susceptible to high ambient temperatures (thermal stress), which negatively affect their growth and productivity, leading to enormous economic losses. In the light of global warming, these losses are expected to increase in the near future. Specifically, the worsening of climate change and the rise in global temperatures have augmented the adverse effects of heat on poultry production worldwide. At present, the world population is approximately 7.9 billion, and it has been predicted to reach 9.3 billion by 2050 and approximately 11 billion by 2100, implying a great demand for protein supply; therefore, strategies to mitigate future poultry challenges must be urgently devised. To date, several mitigation measures have been adopted to minimize the negative effects of heat stress in poultry. Of these, thermal acclimation at the postnatal stage or throughout the embryonic stages has been explored as a promising approach; however, for large-scale application, this approach warrants further investigation to determine the suitable temperature and poultry age. Moreover, molecular mechanisms governing thermal conditioning are poorly understood. To this end, we sought to expand our knowledge of thermal conditioning in poultry, which may serve as a valuable reference to improve the thermotolerance of chickens via nutritional management and vitagene regulation. Vitagenes regulate the responses of poultry to diverse stresses. In recent years, nutritionists have paid close attention to bioactive compounds such as resveratrol, curcumin, and quercetin administered alone or in combination. These compounds activate vitagenes and other regulators of the antioxidant defense system, such as nuclear factor-erythroid 2-related factor 2. Overall, thermal conditioning may be an effective strategy to mitigate the negative effects of heat stress. In this context, the present review synthesizes information on the adverse impacts of thermal stress, elucidating the molecular mechanisms underlying thermal conditioning and its effects on the acquisition of tolerance to acute heat stress in later life. Finally, the role of some polyphenolic compounds, such as resveratrol, curcumin, and quercetin, in attenuating heat stress through the activation of the antioxidant defense system in poultry are discussed.

Early-life thermal stress mediates long-term alterations in hypothalamic microglia.

Stressful environmental events in early life have long-lasting consequences on later stress responses. We previously showed that heat conditioning of 3-day-old chicks during the critical period of heat-response development leads to heat vulnerability later in life. Here we assessed the role of early-life heat stress on the inflammatory response in the chick anterior hypothalamus (AH), focusing on hypothalamic microglia. We identified the microglial cell population in the chick AH using anti-KUL01 and anti-CD45 antibodies. Specific microglial features were also confirmed by expression of their signature genes. Under normal environmental conditions, hypothalamic microglia isolated from lipopolysaccharide (LPS)-injected chicks displayed a classical activated proinflammatory profile accompanied by a decreased homeostatic signature, demonstrating similarity of immune response with mammalian microglial cells. In accordance with our previous observations, conditioning of 3-day-old chicks under high ambient temperature decreased the number of newborn cells in the AH, among them microglial precursors. Although heat exposure did not affect microglial cell viability, it had a long-term impact on LPS-induced inflammatory response. Exposure to harsh heat led to heat vulnerability, and attenuated recruitment of peripheral monocytes and T cells into the AH following LPS challenge. Moreover, heat conditioning altered microglial reactivity, manifested as suppressed microglial activation in response to LPS. Innate immune memory developed by heat conditioning might underlie suppression of the microglial response to LPS challenge. We describe alterations in genome-wide CpG methylation profile of hypothalamic microglia, demonstrating probable epigenetic involvement in the reprogramming of microglial function, leading to heat-induced inflammatory cross-tolerance.

Effects of Thermal Conditioning and Folic Acid on Methylation of the BDNF Promoter Region in Chicks.

This study aimed to investigate the effects of thermal conditioning and folic acid on the methylation levels of the avian brain-derived neurotrophic factor (BDNF) promoter region at the M3 and M9 positions in the early life of broiler chicks. In Experiment 1, male broiler chicks (day 3 of life) were orally injected with methyl cellulose solution with or without folic acid (25 mg). The chicks in the heat-treatment groups were immediately exposed to a high ambient temperature (40±0.5°C) for 12 h, while chicks in the non-heat treatment groups were left in the thermoneutral zone (30±0.5°C). The groups were as follows: 1) no thermal conditioning group without folic acid (control), 2) thermal conditioning group without folic acid, 3) no thermal conditioning group with folic acid, and 4) thermal conditioning group with folic acid. In Experiment 2, treatments were similar to those in Experiment 1, except for the usage of female chicks. After the treatments, the methylation levels of the BDNF promoter in chicks were determined using semiquantitative PCR. There were no significant differences between groups in the levels of methylation at the M3 position in both males and females as a result of thermal conditioning and folic acid treatment. Interestingly, significant effects of thermal conditioning and folic acid treatment on methylation at the M9 position were found. BDNF methylation levels at M9 significantly decreased following thermal conditioning, while folic acid suppressed demethylation in both male and female chicks. These data suggest that folic acid and thermal conditioning affects DNA methylation patterns in the central nervous system of chicks, regardless of sex.

Influence of Thermal Annealing on the Sinterability of Different Grades of Polylactide Microspheres Dedicated for Laser Sintering.

We present a comparison of the influence of the conditioning temperature of microspheres made of medical grade poly(L-lactide) (PLLA) and polylactide with 4 wt % of D-lactide content (PLA) on the thermal and structural properties. The microspheres were fabricated using the solid-in-oil-in-water method for applications in additive manufacturing. The microspheres were annealed below the glass transition temperature (Tg), above Tg but below the onset of cold crystallization, and at two temperatures selected from the range of cold crystallization corresponding to the crystallization of the α' and α form of poly(L-lactide), i.e., at 40, 70, 90, and 120 °C, in order to verify the influence of the conditioning temperature on the sinterability of the microspheres set as the sintering window (SW). Based on differential scanning calorimetry measurements, the SWs of the microspheres were evaluated with consideration of the existence of cold crystallization and reorganization of crystal polymorphs. The results indicated that the conditioning temperature influenced the availability and range of the SWs depending on the D-lactide presence. We postulate the need for an individual approach for polylactide powders in determining the SW as a temperature range free of any thermal events. We also characterized other core powder characteristics, such as the residual solvent content, morphology, particle size distribution, powder flowability, and thermal conductivity, as key properties for successful laser sintering. The microspheres were close to spheres, and the size of the microspheres was below 100 µm. The residual solvent content decreased with the increase of the annealing temperature. The thermal conductivities were 0.073 and 0.064 W/mK for PLA and PLLA microspheres, respectively, and this depended on the spherical shape of the microspheres. The wide angle X-ray diffraction (WAXD) studies proved that an increase in the conditioning temperature caused a slight increase in the crystallinity degree for PLLA microspheres and a clear increase in crystallization for the PLA microspheres.

Effect of thermal conditioning on growth performance and thermotolerance in broilers: A systematic review and meta-analysis.

Thermal conditioning has been introduced as a cost-effective way to improve performance and thermotolerance in broilers. However, since all the trials were performed under various experimental conditions, it appears difficult to draw general conclusions. Therefore, the objective of this study was to quantify the response of broilers to thermal conditioning through a meta-analysis approach. A literature search was conducted on Scopus, PubMed, Scielo, Web of Science, and Google scholar in December 2020. A restricted maximum likelihood random effect model was used to pool the effect sizes from the body weight gain (BWG), feed intake (FI), feed conversion ratio (FCR), and body temperature (Tb). BWG, FI, and Tb were computed using the standardized mean difference (SMD) while FCR was computed using mean differences (MD) with a 95% confidence interval (IC). Growth performances were evaluated during the thermoneutral conditions while Tb was evaluated after either acute or chronic heat stress after early age thermal conditioning. A total of 17 studies were included in the dataset. Thermal conditioning significantly increased BWG (SMD = 0.139, IC = 0.0372-0.2407, P = 0.0074) and FI (SMD = 0.292, IC = 0.108-0.476, P = 0.0019) compared with the control. Additionally, subgroup analysis revealed that overall Tb was significantly reduced under acute heat stress (SMD = -0.455, IC = -0.718 to -0.192, P < 0.001) but not affected during chronic heat stress (SMD = -0.115, IC = -0.651 to -0.420, P = 0.6729). In conclusion, thermal conditioning significantly increased the broiler's BWG and FI under thermoneutral conditions and can help in reducing Tb under acute heat stress.

Thermal conditioning improves quality and speed of keratinocyte sheet production for burn wound treatment.

BACKGROUND AIMS: Cultured patient-specific keratinocyte sheets have been used clinically since the 1970s for the treatment of large severe burns. However, despite significant developments in recent years, successful and sustainable treatment is still a challenge. Reliable, high-quality grafts with faster availability and a flexible time window for transplantation are required to improve clinical outcomes. METHODS: Keratinocytes are usually grown in vitro at 37°C. Given the large temperature differences in native skin tissue, the aim of the authors' study was to investigate thermal conditioning of keratinocyte sheet production. Therefore, the influence of 31°C, 33°C and 37°C on cell expansion and differentiation in terms of proliferation and sheet formation efficacy was investigated. In addition, the thermal effect on the biological status and thus the quality of the graft was assessed on the basis of the release of wound healing-related biofactors in various stages of graft development. RESULTS: The authors demonstrated that temperature is a decisive factor in the production of human keratinocyte sheets. By using specific temperature ranges, the authors have succeeded in optimizing the individual manufacturing steps. During the cell expansion phase, cultivation at 37°C was most effective. After 6 days of culture at 37°C, three times and six times higher numbers of viable cells were obtained compared with 33°C and 31°C. During the cell differentiation and sheet formation phase, however, the cells benefited from a mildly hypothermic temperature of 33°C. Keratinocytes showed increased differentiation potential and formed better epidermal structures, which led to faster biomechanical sheet stability at day 18. In addition, a cultivation temperature of 33°C resulted in a longer lasting and higher secretion of the investigated immunomodulatory, anti-inflammatory, angiogenic and pro-inflammatory biofactors. CONCLUSIONS: These results show that by using specific temperature ranges, it is possible to accelerate the large-scale production of cultivated keratinocyte sheets while at the same time improving quality. Cultivated keratinocyte sheets are available as early as 18 days post-biopsy and at any time for 7 days thereafter, which increases the flexibility of the process for surgeons and patients alike. These findings will help to provide better clinical outcomes, with an increased take rate in severe burn patients.

Repeated thermal conditioning during the neonatal period affects behavioral and physiological responses to acute heat stress in chicks.

OBJECTIVE: The aim of the present study was to investigate the effects of repeated thermal conditioning (RTC) at an early age on physiological and behavioral responses in chicks. METHODS: Birds were assigned to one of the four treatments in which the RTC was exposure to 40 °C for 15 min daily. The treatments were 1) no thermal conditioning (control); 2) early exposure group (EE; RTC from 2 to 4 days of age); 3) later exposure group (LE; RTC from 5 to 7 days of age); or 4) both early and later exposure (BE; RTC from 2 to 7 days of age). All groups of chicks were challenged with high ambient temperature (40 °C for 15 min) at two weeks of age. RESULTS: During heat challenge, initiation times of dissipation behaviors (panting and wing-drooping) were measured. Rectal temperature and respiration rate were measured after and before heat challenge. Hypothalamic samples and blood were collected at the end of heat challenges. Initiation times of dissipation behaviors and rectal temperature were not affected by the treatments. Increases in respiration rate in response to heat challenge were suppressed by early RTC treatment. There was no clear pattern of glucose levels in relation to thermal conditioning, whereas plasma corticosterone levels were decreased by early treatment (EE and BE groups). Hypothalamic thyrotropin releasing hormone gene expression was suppressed by early and later thermal conditioning and suppressed further by both early and later exposure. Neuropeptide Y gene expression in the BE group was lower than in the other groups, with a similar trend for corticotropin releasing hormone expression. CONCLUSION: Our results suggest that the effect of repeated thermal conditioning on the central thermoregulatory system depends on the number of times that chicks experienced conditioning. In addition, repeated thermal conditioning has greater effects on the acquisition of thermotolerance when conditioning occurs in chicks of two to four days of age in comparison with chicks of five to seven days of age.

The effect of nasal shape on the thermal conditioning of inhaled air: Using clinical tomographic data to build a large-scale statistical shape model.

In this paper, we investigate the heating function of the nasal cavity qualitatively, using a high-quality, large-scale statistical shape model. This model consists of a symmetrical and an asymmetrical part and provides a new and unique way of examining changes in nasal heating function resulting from natural variations in nasal shape (as obtained from 100 clinical CT scans). Data collected from patients suffering from different nasal or sinus-related complaints are included. Parameterized models allow us to investigate the effect of continuous deviations in shape from the mean nasal cavity. This approach also enables us to avoid many of the compounded effects on flow and heat exchange, which one would encounter when comparing different patient-specific models. The effects of global size, size-related features, and turbinate size are investigated using the symmetrical shape model. The asymmetrical model is used to investigate different types of septal deviation using Mladina's classification. The qualitative results are discussed and compared with findings from the existing literature.

Effects of Thermal Conditioning on Changes in Hepatic and Muscular Tissue Associated With Reduced Heat Production and Body Temperature in Young Chickens.

Effects of increased summer temperatures on poultry production are becoming more pronounced due to global warming, so it is important to consider approaches that might reduce heat stress in chickens. Thermal conditioning in chickens in the neonatal period can improve thermotolerance and reduce body temperature increases when birds are exposed to high ambient temperature later in life. The objective of this study was to investigate physiological and molecular changes associated with heat production and hence body temperature regulation under high ambient temperatures in thermally conditioned chicks. Three-day-old broiler chicks (Chunky) were thermally conditioned by exposure to a high ambient temperature (40°C) for 12 h while control chicks were kept at 30°C. Four days after the treatment, both groups were exposed to 40°C for 15 or 90 min. The increase in rectal temperature during 90 min of exposure to a high ambient temperature was less in thermally conditioned than control chicks. At 15-min of re-exposure treatment, gene expression for uncoupling protein and carnitine palmitoyletransferase 1, key molecules in thermogenesis and fatty acid oxidation, were significantly higher in pectoral muscle of control chicks but not conditioned chicks. Hepatic argininosuccinate synthase (ASS) decreased and hepatic argininosuccinate lyase (ASL) increased after reexposure to a high temperature. The concentrations of hepatic arginosuccinic acid, and ASS and ASL expression, were upregulated in conditioned chicks compared with the control chicks, indicating activity of the urea cycle could be enhanced to trap more energy to reduce heat production in conditioned chicks. These results suggest thermal conditioning can reduce the increase in heat production in muscles of chickens that occurs in high ambient temperatures to promote sensible heat loss. Conditioning may also promote energy trapping process in the liver by altering the heat production system, resulting in an alleviation of the excessive rise of body temperature.

Thermal stimulation of Ross®-lineage embryos on a commercial scale / Estimulação térmica dos embriões da linhagem Ross® em escala comercial

ABSTRACT: Artificial incubation is an essential process to obtain healthy birds with good performance; nevertheless, it requires sustained improvement. During this process, incubation temperature is considered a critical factor, which has been studied. The objective of this study was to evaluate the development of Ross(r) embryos after hot and cold thermal stimulation. To this end, temperatures 1.39ºC above the standard temperature and a temperature fixed at 36.00ºC that varied 1.00 to 0.30ºC below the standard temperature were applied during the final embryonic development period (days 14 to 18) for three hours, on a commercial scale. Results revealed that hot and cold thermal stimulations did not cause embryo mortality; the hatching and chick quality index were maintained and even increased. Therefore, we believe that thermal stimulation has the potential to improve hatchery index, and thus grange performance; however, adjustments are needed, varying according to each individual hatchery, before it can used as a protocol.

RESUMO: A incubação artificial é um processo fundamental para obtenção de aves saudáveis e com bom rendimento, contudo, necessita de constantes melhorias. Nesse processo, a temperatura de incubação é considerada um fator crítico e vem sendo estudada. O objetivo deste trabalho foi avaliar o desenvolvimento de embriões da linhagem Ross(r) frente a estimulações térmicas por calor e por frio. Para tanto, temperaturas de 1,39ºC acima da temperatura padrão e de 36,00ºC fixos, abaixo da temperatura padrão (variando de 1,00 a 0,30ºC), foram aplicadas no último período de desenvolvimento embrionário (14º ao 18º dia) por três horas de duração, em escala comercial. Com esse experimento, percebeu-se que as estimulações térmicas por calor e por frio não causaram mortalidade embrionária, mantiveram e até aumentaram os índices de eclosão, bem como a qualidade do pintinho. Dessa forma, acredita-se que as estimulações térmicas têm potencial de melhorar os índices do incubatório, e consequentemente o desempenho na granja, contudo necessita de ajustes, que dependem de cada incubatório para ser utilizada como protocolo.

Estratégias para minimizar os efeitos de um ambiente térmico adverso para frangos de corte / Strategies to minimize the adverse thermal environment in broiler chickens

O calor limita a produção de frangos de corte. Este trabalho avaliou as interações entre o termocondicionamento precoce (TCP) e o uso do equilíbrio eletrolítico (EE) sobre o desempenho e as características da carcaça das aves. Assim, o balanço eletrolítico (BE = Na + K - Cl) foi ajustado em 350mEq/kg, e a relação eletrolítica (K+Cl)/Na em 3:1, pelo programa PPFR. O experimento foi realizado no setor de Zootecnia da Faculdade de Medicina Veterinária de Araçatuba/Unesp. Alojaram-se 240 pintos machos de um dia de idade, linhagem Cobb-500(r), em bateria metálica com 24 divisões e aquecimento elétrico automático. O delineamento experimental foi inteiramente ao acaso, em arranjo fatorial 2x2, totalizando quatro tratamentos com seis repetições e 10 aves por parcela experimental. O TCP ocorreu no quinto dia de idade, por 24 horas, a 36,5°C, em metade do lote. Posteriormente, as aves foram transferidas para um galpão climatizado e alojadas em boxes, da mesma forma que a outra parcela que não passou pelo TCP. Avaliaram-se os efeitos do estresse calórico crônico (seis horas a 32°C) aplicado do 35º ao 39º dia de idade. Foram monitoradas a temperatura e a umidade do ar, bem como a temperatura de globo negro. Alimentação e água foram fornecidas ad libitum. Calcularam-se os dados de desempenho zootécnico e a taxa de mortalidade das aves. Submeteram-se os resultados à análise de variância e à comparação de médias pelo teste de Tukey. Não houve interação entre o TCP e o EE sobre o desempenho e as características da carcaça das aves. As estratégias, TCP e EE, mostraram-se ineficazes para amenizar os efeitos do estresse calórico crônico em frangos de corte.

Heat limits the production of broilers. This study evaluated the interactions between early thermal conditioning (ETC) and the electrolyte balance (EB) on performance and characteristics of birds' carcass. Thus, the electrolyte balance (BE = Na+ + K+ - Cl-), was set to 350mEq/kg and electrolyte ratio (K+ + Cl-)/ Na+ in 3:1 by the PPFR program. The experiment was carried out at the Animal Science department of the Faculty of Veterinary Medicine of Araçatuba / UNESP. 240 1 day old Cobb-500(r) male chicks were used, lodged in metal batteries with 24 divisions and automatic electric heating. The experimental design was completely randomized in a 2x2 factorial arrangement, totaling 4 treatments with 6 replicates and 10 birds per experimental plot. ETC occurred on the 5th day of age for 24 hours at 36.5°C in half of the batch. Subsequently, these birds were transferred to an environmentally controlled shed and were grown in boxes, in the same way as the other half that had not received ETC treatment. The effects of chronic heat stress (6 hours at 32°C) applied from the 35th through the 39th day of age were evaluated. Temperature and air humidity, as well black-globe temperature were monitored electronically. Food and water were provided ad libitum. The growth performance data and the mortality rate of birds were measured. The results were subjected to analysis of variance and comparison of means was done by Tukey test. There were no interaction effects between ETC and EB on performance and characteristics of birds' carcass. The ETC and EB strategies proved to be ineffective to mitigate the effects of chronic heat stress in broilers.

Flash Thermal Conditioning of Olive Pastes during the Oil Mechanical Extraction Process: Cultivar Impact on the Phenolic and Volatile Composition of Virgin Olive Oil.

The concentration of phenolic and volatile compounds in virgin olive oil (VOO) is closely related to the different operative conditions applied to the mechanical extraction process of the olive oil. However, the great qualitative and quantitative variability of these compounds indicates an important role played by genetic and agronomic aspects. A heat exchanger was placed in front of a traditional, covered malaxer to study the impact of flash thermal conditioning (FTC) of olive paste on the quality of VOO, which is highly influenced by phenolic release and aroma generation. The VOO flash thermal conditioning of five major Italian cultivars showed a higher concentration of phenols (range of increase percentage, 9.9-37.3%) compared to the control trials, whereas the FTC treatment featured a differentiated impact on the volatile fractions, associated with the genetic origins of the olives.