Global prevalence of potentially pathogenic free-living amoebae in sewage and sewage-related environments-systematic review with meta-analysis.

Free-living amoebae (FLA) include amphizoic microorganisms important in public health, widely isolated from air, water, and soil. However, its occurrence in sewage-related environments still needs to be systematically documented. This study summarizes the occurrence of FLA in sewage-related environments through a systematic review with meta-analysis. A total of 1983 scientific article were retrieved from different databases, of which 35 were selected and analyzed using a random effects forest plot model with a 95% confidence interval (IC). The pooled overall prevalence of FLA in sewage across 12 countries was 68.96% (95% IC = 58.5-79.42). Subgroup analysis indicates high prevalence in all environments analyzed, including sewage water from the sewage treatment plant (81.19%), treated sewage water (75.57%), sewage-contaminated water (67.70%), sediment contaminated by sewage (48.91%), and sewage water (47.84%). Prevalence values of Acanthamoeba spp., Hartmanella/Vermamoeba spp., and Naegleria spp. are 47.48%, 28.24%, and 16.69%, respectively. Analyzing the species level, the distribution is as follows: Acanthamoeba palestinensis (88%), A. castellanii (23.74%), A. astronyxis (19.18%), A. polyphaga (13.59%), A. culbertsoni (12.5%), A. stevensoni (8.33%), A. tubiashi (4.35%) and A. hatchetti (1.1%), Naegleria fowleri (28.4%), N. gruberi (25%), N. clarki (8.33%), N. australiensis (4.89%) and N. italica (4.29%), Hartmannella/Vermamoeba exundans (40%) and H.V. vermiform (32.61%). Overall, our findings indicate a high risk associated with sewage-related environments, as the prevalence of FLA, including pathogenic strains, is high, even in treated sewage water. The findings of this study may be valuable both for risk remediation actions against amoebic infections and for future research endeavors.

Coagulative removal of polystyrene microplastics from aqueous matrices using FeCl3-chitosan system: Experimental and artificial neural network modeling.

Effluent from sewage treatment plants (STPs) is a significant source of microplastics (MPs) re-entry into the environment. Coagulation-flocculation-sedimentation (CFS) process as an initial tertiary treatment step requires investigation for coagulative MPs removal from secondary-treated sewage effluents. In this study, experiments were conducted on synthetic water containing 25 mg/L polystyrene (PS) MPs using varying dosages of FeCl3 (1-10 mg/L) and chitosan (0.25-9 mg/L) to assess the effect of process parameters, such as pH (4-8), stirring speed (0-200 rpm), and settling time (10-40 min). Results revealed that ∼89.3% and 21.4% of PS removal were achieved by FeCl3 and chitosan, respectively. Further, their combination resulted in a maximum of 99.8% removal at favorable conditions: FeCl3: 2 mg/L, chitosan: 7 mg/L, pH: 6.3, stirring speed: 100 rpm, and settling time: 30 min, with a statistically significant (p < 0.05) effect. Artificial neural network (ANN) validated the experimental results with RMSE = 1.0643 and R2 = 0.9997. Charge neutralization, confirmed by zeta potential, and adsorption, ascertained by field-emission scanning electron microscope (FESEM) and Fourier-transform infrared spectroscopy (FTIR), were primary mechanisms for efficient PS removal. For practical considerations, the application of the FeCl3-chitosan system on the effluents from moving bed biofilm reactor (MBBR) and sequencing batch reactor (SBR)-based STPs, spiked with PS microbeads, showed > 98% removal at favorable conditions.

The combined effects of treated sewage discharge and land use on rivers.

Freshwater ecosystems are increasingly threatened by multiple anthropogenic stressors. Release of treated sewage effluent and pollution from agricultural or urban sources can independently reduce water quality with implications for ecological communities. However, our knowledge of the combined effects of these stressors is limited. We performed a field study to quantify the combined effect of treated sewage discharge and land use on nutrient concentrations, sewage fungus presence and communities of macroinvertebrates and benthic algae. Over three seasons in four rivers we found that a model which included an interaction between sewage pollution and time of the year (i.e. months) was the best predictor of nutrient concentrations and the abundance of algae and sewage fungus. Both macroinvertebrate and algae communities shifted downstream of sewage input. Specifically, more tolerant groups, such as cyanobacteria and oligochaetes, were more abundant. The EPT (Ephemeroptera, Plecoptera and Tricoptera) water quality score was best explained by an interaction between month and agriculture in the surrounding landscape. Overall, our results show that sewage discharge has a significant impact on water quality and benthic riverine communities, regardless of the surrounding land uses. Agricultural inputs, however, could be more important than treated sewage discharge in reducing the abundance of sensitive invertebrate taxa. We need both improvements to wastewater treatment processes and reductions in agricultural pollution to reduce threats to vulnerable freshwater communities.

Impact of treated sewage on meiobenthic nematodes: a case study from the Tunisian Refining Industries Company / Impacto de esgoto tratado em nematoides meiobentônicos: um estudo de caso da Tunisian Refining Industries Company

The main objective of the current study was to assess the impact of the water taken from the 'Tunisian Refining Industries Company' on meiobenthic nematodes, before and after a series of treatments in decantation basins followed by its discharge in Bizerte bay, Tunisia. The comparison of environmental parameters of the two types of water was clearly indicative of an improvement in the quality of treated waters after a significant reduction in their loads in hydrocarbons. Overall, the water retained a good quality after being treated by 'Tunisian Refining Industries Company' before discharge in the sea. At the end of the experiment, differential responses were observed according to the richness of sediment in organic matter and hydrocarbons. Thus, it was apparent that the nematode assemblage exposed to the treated waters was closer to controls and associated to higher values of abundance, than that under untreated ones. It was also assumed that the species Microlaimus honestus De Man, 1922, Paramonohystera proteus Wieser, 1956 and Cyartonema germanicum Juario, 1972 are sensitive bioindicators of bad environmental statues and of hydrocarbon presence in the environment. On the other hand, Metoncholaimus pristiurus (Zur Strassen, 1894) Filipjev, 1918 would rather be classified as a positive bioindicative species of this type of pollutants.

O principal objetivo do presente estudo foi avaliar o impacto da água retirada da "Tunisian Refining Industries Company" sobre os nematoides meiobentônicos, antes e depois de uma série de tratamentos em bacias de decantação seguidos de seu descarte na baía de Bizerte, Tunísia. A comparação dos parâmetros ambientais dos dois tipos de água foi claramente indicativa de uma melhoria na qualidade das águas tratadas após uma redução significativa das suas cargas em hidrocarbonetos. No geral, a água reteve uma boa qualidade após ser tratada pela "Tunisian Refining Industries Company" antes da descarga no mar. Ao final do experimento, foram observadas respostas diferenciais de acordo com a riqueza de sedimentos em matéria orgânica e hidrocarbonetos. Assim, ficou claro que a assembleia de nematoides exposta às águas tratadas estava mais próxima dos controles e associada a maiores valores de abundância do que nas não tratadas. Também foi assumido que as espécies Microlaimus honestus De Man, 1922, Paramonohystera proteus Wieser, 1956 e Cyartonema germanicum Juario, 1972 são bioindicadores sensíveis de más estátuas ambientais e da presença de hidrocarbonetos no meio ambiente. Por outro lado, Metoncholaimus pristiurus (Zur Strassen, 1894) Filipjev, 1918 preferiria ser classificado como uma espécie bioindicativa positiva deste tipo de poluentes.

Impact of treated sewage on meiobenthic nematodes: a case study from the Tunisian Refining Industries Company

Abstract The main objective of the current study was to assess the impact of the water taken from the Tunisian Refining Industries Company on meiobenthic nematodes, before and after a series of treatments in decantation basins followed by its discharge in Bizerte bay, Tunisia. The comparison of environmental parameters of the two types of water was clearly indicative of an improvement in the quality of treated waters after a significant reduction in their loads in hydrocarbons. Overall, the water retained a good quality after being treated by Tunisian Refining Industries Company before discharge in the sea. At the end of the experiment, differential responses were observed according to the richness of sediment in organic matter and hydrocarbons. Thus, it was apparent that the nematode assemblage exposed to the treated waters was closer to controls and associated to higher values of abundance, than that under untreated ones. It was also assumed that the species Microlaimus honestus De Man, 1922, Paramonohystera proteus Wieser, 1956 and Cyartonema germanicum Juario, 1972 are sensitive bioindicators of bad environmental statues and of hydrocarbon presence in the environment. On the other hand, Metoncholaimus pristiurus (Zur Strassen, 1894) Filipjev, 1918 would rather be classified as a positive bioindicative species of this type of pollutants.

Resumo O principal objetivo do presente estudo foi avaliar o impacto da água retirada da Tunisian Refining Industries Company sobre os nematoides meiobentônicos, antes e depois de uma série de tratamentos em bacias de decantação seguidos de seu descarte na baía de Bizerte, Tunísia. A comparação dos parâmetros ambientais dos dois tipos de água foi claramente indicativa de uma melhoria na qualidade das águas tratadas após uma redução significativa das suas cargas em hidrocarbonetos. No geral, a água reteve uma boa qualidade após ser tratada pela Tunisian Refining Industries Company antes da descarga no mar. Ao final do experimento, foram observadas respostas diferenciais de acordo com a riqueza de sedimentos em matéria orgânica e hidrocarbonetos. Assim, ficou claro que a assembleia de nematoides exposta às águas tratadas estava mais próxima dos controles e associada a maiores valores de abundância do que nas não tratadas. Também foi assumido que as espécies Microlaimus honestus De Man, 1922, Paramonohystera proteus Wieser, 1956 e Cyartonema germanicum Juario, 1972 são bioindicadores sensíveis de más estátuas ambientais e da presença de hidrocarbonetos no meio ambiente. Por outro lado, Metoncholaimus pristiurus (Zur Strassen, 1894) Filipjev, 1918 preferiria ser classificado como uma espécie bioindicativa positiva deste tipo de poluentes.

Impact of treated sewage on meiobenthic nematodes: a case study from the Tunisian Refining Industries Company / Impacto de esgoto tratado em nematoides meiobentônicos: um estudo de caso da Tunisian Refining Industries Company

The organization of the roots, ganglia and the peripheral distribution of the cranial nerves of the fully formed embryos of Oreochromis niloticus are examined in the transverse serial sections. These nerves carry fibers, which were also analyzed. The results of this study demonstrated that the glossopharyngeal nerve originates by means of only one root, which leaves the cranium through the glossopharyngeal foramen. This nerve gives fibers (visceromotor) to the first internal and external levator arcus branchialis muscles. There is a single epibranchial (petrosal) ganglion located extracranially. Nervus glossopharyngeus has three rami; pharyngeus, pretramticus and posttrematicus. The ramus pharyngeus carries only viscerosensory fibers; general for the pharyngeal epithelium and special ones for the pseudobranch. General viscerosensory fibers are also carried by rami pretrematicus and posttrematicus for the pharyngeal epithelial lining. The special sensory fibers are carried by the ramus pretrematicus for the taste buds and by ramus posttrematicus for the gill filaments. The ramus pretrematicus also carries visceromotor fibers for the first adductor arcus branchialis and to the first obliquus ventralis muscles.

A organização das raízes, gânglios e a distribuição periférica dos nervos cranianos dos embriões totalmente formados de Oreochromis niloticus são examinados nas seções transversais seriais. Esses nervos carregam fibras, que também foram analisadas. Os resultados deste estudo demonstraram que o nervo glossofaríngeo se origina por meio de apenas uma raiz, que sai do crânio pelo forame glossofaríngeo. Este nervo fornece fibras (visceromotoras) para os primeiros músculos levantadores do arco branquial interno e externo. Existe um único gânglio epibranquial (petroso) localizado extracranialmente. Nervus glossopharyngeus tem três ramos; faríngeo, pretramticus e póstrematicus. O ramo faríngeo contém apenas fibras viscerossensoriais ­ gerais para o epitélio faríngeo e especiais para o pseudobrânquio. Fibras viscerossensoriais gerais também são transportadas por ramos pretrematicus e posttrematicus para o revestimento epitelial da faringe. As fibras sensoriais especiais são transportadas pelo ramus pretrematicus para as papilas gustativas e pelo ramus posttrematicus para os filamentos branquiais. O ramo pretrematicus também carrega fibras visceromotoras para o primeiro adutor arcus branchialis e para o primeiro músculo oblíquo ventral.

Cryptic night-time trace metal and metalloid contamination in an intensively cultivated coastal catchment.

Detailed, high resolution time-series observations were performed to investigate sources, diel cycling, natural attenuation, and loadings of dissolved trace metals/metalloids in a subtropical headwater stream draining intensive horticulture in Australia. A transect of ∼3 km away from the source (farms) showed >75% reduction in concentration and loads of most trace elements. Mercury and arsenic had elevated loads downstream relative to other elements. Hourly time-series sampling revealed elevated creek discharge at night, accompanied by elevated nickel, selenium, copper, and mercury loads. Inputs from groundwater or treated sewage used for irrigation within the catchment are likely sources. Groundwater bore and treated sewage samples were highly contaminated with either zinc, copper, or mercury. Comparisons of daily and hourly samples indicated common sampling strategies can underestimate horticultural contaminant loadings. Load estimates for mercury and copper derived from hourly samples were 1.6- to 7- fold greater than loads from daily sample data collected over 79 days with varying rainfall. These high contaminant concentrations and loads are of concern to food products receiving irrigation and protected waterbodies downstream.

Comparison of Nanofiltration with Reverse Osmosis in Reclaiming Tertiary Treated Municipal Wastewater for Irrigation Purposes.

This study compares the performance of nanofiltration (NF) and reverse osmosis (RO) for the reclamation of ultrafiltered municipal wastewater for irrigation of food crops. RO and NF technologies were evaluated at different applied pressures; the performance of each technology was evaluated in terms of water flux, recovery rate, specific energy consumption and quality of permeate. It was found that the permeate from the reverse osmosis (RO) process complied with Food and Agriculture Organization (FAO) standards at pressures applied between 10 and 18 bar. At an applied pressure of 20 bar, the permeate quality did not comply with irrigation water standards in terms of chloride, sodium and calcium concentration. It was found that nanofiltration process was not suitable for the reclamation of wastewater as the concentration of chloride, sodium and calcium exceeded the allowable limits at all applied pressures. In the reverse osmosis process, the highest recovery rate was 36%, which was achieved at a pressure of 16 bar. The specific energy consumption at this applied pressure was 0.56 kWh/m3. The lowest specific energy of 0.46 kWh/m3 was achieved at an applied pressure of 12 bar with a water recovery rate of 32.7%.

Neural stem cell-based in vitro bioassay for the assessment of neurotoxic potential of water samples.

Intensive agriculture activities, industrialization and growing numbers of wastewater treatment plants along river banks collectively contribute to the elevated levels of neurotoxic pollutants in natural water reservoirs across Europe. We established an in vitro bioassay based upon neural stem cells isolated from the subventricular zone of the postnatal mouse to evaluate the neurotoxic potential of raw wastewater, treated sewage effluent, groundwater and drinking water. The toxic potential of water samples was evaluated employing viability, proliferation, differentiation and migration assays. We found that raw wastewater could reduce the viability and proliferation of neural stem cells, and decreased the neuronal and astrocyte differentiation, neuronal neurite growth, astrocyte growth and cell migration. Treated sewage water also showed inhibitory effects on cell proliferation and migration. Our results indicated that relatively high concentrations of nitrogenous substances, pesticides, mercuric compounds, bisphenol-A, and phthalates, along with some other pollutants in raw wastewater and treated sewage water, might be the reason for the neuroinhibitory effects of these water samples. Our model successfully predicted the neurotoxicity of water samples collected from different sources and also revealed that the incomplete removal of contaminants from wastewater can be problematic for the developing nervous system. The presented data also provides strong evidence that more effective treatments should be used to minimize the contamination of water before release into major water bodies which may be considered as water reservoirs for human usage in the future.

Heavy metal stabilization and improved biochar generation via pyrolysis of hydrothermally treated sewage sludge with antibiotic mycelial residue.

Hydrothermally treated sewage sludge was pyrolyzed at temperatures of 300, 500, and 700 °C with antibiotic mycelial residue addition ratios of 0, 10, 25, and 50 wt%. The results showed that co-pyrolysis could obviously improve biochar properties. Specifically, adding antibiotic mycelial residue increased the aromaticity and raised the higher heating value of the biochar, which indicates its better potential as fuel. The enrichment in functional groups improved the surface properties of biochar, indicating its better applicability. Additionally, the heavy metal concentrations in biochar were diluted by adding antibiotic mycelial residue, which led to lower toxic inputs to the environment. Moreover, heavy metals were transformed to more stable fractions after co-pyrolysis. A higher pyrolysis temperature and greater antibiotic mycelial residue amounts led to better immobilization of heavy metals, thus preventing their leaching to the environment. This work proposes a promising technique for the synergetic treatment of sewage sludge and antibiotic mycelial residue for improved biochar formation.

Carbide Derived Carbon (CDC) as novel adsorbent for ibuprofen removal from synthetic water and treated sewage effluent.

PURPOSE: Pharmaceuticals are becoming one of the largest environmental concerns when it comes to the water treatment industry. Increased usage of these chemicals poses a serious risk to ecology and human health due to their leakage into surface waters. In the present study, carbide derived carbon (CDC) was used for the first time as a new adsorbent to remove ibuprofen from synthetic water and wastewater effluent. METHODS: The morphology, chemical composition, surface area and surface charge of the CDC particles were investigated using the transmission electron microscopy, scanning electron microscopy, energy dispersive spectroscopy, Fourier transform infrared spectroscopy, BET analysis and zeta potential measurements. The effects of CDC dosage, temperature, initial pH and agitation speed on the adsorption process were examined by using batch adsorption experiments. Moreover, the adsorption kinetics, thermodynamics, and isotherms were investigated. RESULTS: Adsorption and kinetic equilibrium data demonstrate that the adsorption of ibuprofen onto the CDC obeys the Langmuir isotherm model and the kinetics follow the pseudo-2nd order mechanism. The thermodynamic results reveal that ibuprofen adsorption is endothermic and spontaneous. The ibuprofen removal by CDC was mainly controlled by the electrostatic forces at high pH of the feed solution and by the dispersive interactions in acidic media. The ibuprofen removal is promoted at high temperature, high agitation speed and low pH. The highest adsorption capacity of ibuprofen onto the CDC was 367 mg/g at pH 3. Furthermore, the CDC efficiently removed ibuprofen from spiked treated sewage effluent. CONCLUSIONS: The obtained data indicate that the CDC provides a fast and efficient adsorptive removal of ibuprofen both from a model aqueous solution and treated sewage effluent.

Simultaneous heavy metal immobilization and antibiotics removal during synergetic treatment of sewage sludge and pig manure.

The safe handling of heavy metals and antibiotics during waste disposal has attracted wide attention. In the present study, hydrothermally treated sewage sludge was used for co-pyrolysis with different concentration ratios of pig manure at 600 °C for heavy metal immobilization and antibiotic removal. Heavy metals (except Cd) were mainly retained in the biochar samples due to a high degree of decomposition characteristic of organic matter. Pyrolysis significantly immobilized the heavy metals via converting unstable F1 + F2 + F3 fractions (acid-soluble fraction + reducible fraction + oxidizable fraction) to stable F4 fraction (residual fraction), and more pig manure addition led to improved immobilization performance. After co-pyrolysis, the potential environmental risk of feedstocks reduced significantly and the addition of 50 wt.% of pig manure gave a minimum potential ecological risk index of 10.36 with a low risk of contamination. In addition, six types of antibiotics in feedstocks were decomposed completely during pyrolysis. The co-pyrolysis process showed numerous advantages in the synthetic treatment of sewage sludge and pig manure by reducing the heavy metal toxicity and antibiotic levels.

Public acceptability of treated wastewater reuse in Saudi Arabia: Implications for water management policy.

Treated wastewater reuse is increasingly important for sustainable water resource management, especially in water-stressed countries located in the world's arid regions that rely on groundwater and desalination process for meeting their water demands. This study investigates the socio-demographic variables influencing public perceptions of reusing grey and mixed wastewater for non-domestic uses: firefighting, swimming pools, and car washing. Data were collected from 624 households in the Dammam Metropolitan Area, Saudi Arabia using a structured questionnaire and analyzed using descriptive and inferential statistics. The results from logistic regression indicates that the likelihood of a household to accept reusing treated mixed wastewater is influenced by gender with odds ratio (OR) of 2.71-2.18, residential location (OR = 1.32-1.03), age (OR = 1.22-0.18) and educational level (OR = 1.33-0.98), with a tendency for more acceptance of treated grey wastewater than mixed wastewater. These findings showcase the difficulty that the country could face concerning the public acceptance of treated wastewater for non-domestic uses to augment current freshwater sources even among the educated class. This study is significant because sustainably meeting the country's rising water demands requires the stringent implementation of strategic wastewater reuse policy, including bold steps towards wastewater streams segregation, and intensive public awareness campaigns to change negative perceptions on treated sewage effluent. This study concludes that a substantial reduction in the country's reliance on costly desalinated water and fast depleting non-renewable groundwater requires complete reuse and recycling of treated wastewater for wider non-conventional purposes.

Growth and antibiotic resistance acquisition of Escherichia coli in a river that receives treated sewage effluent.

Wastewater treatment plants could discharge Escherichia coli and antibiotic resistant bacteria to the environment adjacent to, or downstream of their discharge point. However, their discharge also contains nutrients which could promote growth of E. coli in water environments. This study was done to clarify the potential of growth and antibiotic resistance acquisition of E. coli in a river environment. Levels of E. coli were monitored in a river that receives treated sewage effluent for over four years. River water, periphyton and sediment samples were collected at sites upstream and downstream of treated sewage inflow. Concentrations of E. coli increased in river water and periphyton at the sites downstream of the treated sewage inflow, although levels of E. coli were very low or below detection limit in the treated sewage samples. Concentrations of Chlorophyll a increased at the downstream sites, likely due to nutrient input from the treated sewage. Based on pulsed field gel electrophoresis, identical genotype occurred at multiple sites both upstream and downstream of the treated sewage inflow. However, strains resistant to antibiotics such as ampicillin, cefazolin, ciprofloxacin, and chloramphenicol were more frequently obtained from the downstream sites than the upstream sites. Multidrug resistant E. coli strains were detected in periphyton and sediment samples collected at the downstream sites. Non-resistant strains with PDGE genotype identical to the multi-drug strains were also detected, indicating that E. coli might have become resistant to antibiotics by acquiring resistance genes via horizontal gene transfer. Laboratory incubation experiment showed the growth of E. coli in periphyton or sediment-fed river water samples. These results suggest that the wastewater treatment inflow did not directly provide E. coli to the river water, but could promote the growth of periphyton, which could lead to the elevated levels of E. coli and the emergence of antibiotic resistant E. coli.

Residual effects of treated effluent diversion on a seaweed farm in a tidal strait using a multi-nested high-resolution 3-D circulation-dispersal model.

A high-resolution 3-D model was developed to assess the impact of a diversion outfall at the Tarumi Sewage Treatment Plant (TSTP) on an adjacent seaweed farm in Osaka Bay, Japan. The model was extensively validated to ensure a reasonable agreement with in situ observations. The western part of the farm is largely influenced by tidal currents, whereas the eastern area is mainly affected by subtidal residual currents that are primarily due to surface wind stress. The released effluent is transported by counterclockwise residual circulation formed off the TSTP. The model reveals that the diversion adequately suppresses the influence on the farm. While the instantaneous effluent concentration is diminished by about 50%, the effluent accumulated on the farm decreased from 2.83 × 104 m3 to 2.01 × 104 m3 due to the diversion, demonstrating an approximately 28% reduction of the effluent from the TSTP by the diversion outfall.

Spatial and Temporal Microbial Patterns in a Tropical Macrotidal Estuary Subject to Urbanization.

Darwin Harbour in northern Australia is an estuary in the wet-dry tropics subject to increasing urbanization with localized water quality degradation due to increased nutrient loads from urban runoff and treated sewage effluent. Tropical estuaries are poorly studied compared to temperate systems and little is known about the microbial community-level response to nutrients. We aimed to examine the spatial and temporal patterns of the bacterial community and its association with abiotic factors. Since Darwin Harbour is macrotidal with strong seasonal patterns and mixing, we sought to determine if a human impact signal was discernible in the microbiota despite the strong hydrodynamic forces. Adopting a single impact-double reference design, we investigated the bacterial community using next-generation sequencing of the 16S rRNA gene from water and sediment from reference creeks and creeks affected by effluent and urban runoff. Samples were collected over two years during neap and spring tides, in the dry and wet seasons. Temporal drivers, namely seasons and tides had the strongest relationship to the water microbiota, reflecting the macrotidal nature of the estuary and its location in the wet-dry tropics. The neap-tide water microbiota provided the clearest spatial resolution while the sediment microbiota reflected current and past water conditions. Differences in patterns of the microbiota between different parts of the harbor reflected the harbor's complex hydrodynamics and bathymetry. Despite these variations, a microbial signature was discernible relating to specific effluent sources and urban runoff, and the composite of nutrient levels accounted for the major part of the explained variation in the microbiota followed by salinity. Our results confirm an overall good water quality but they also reflect the extent of some hypereutrophic areas. Our results show that the microbiota is a sensitive indicator to assess ecosystem health even in this dynamic and complex ecosystem.

Removal characteristics and fluctuation of norovirus in a pilot-plant by an ultrafiltration membrane for the reclamation of treated sewage.

When ultrafiltration (UF) membrane processes that are able to effectively reduce viruses are installed in a waste water reclamation system, the security of sanitation safety for water-borne diseases is essential. It is important to understand the behaviour of enteric viruses such as Adenovirus, Rotavirus and Norovirus (NV), the detection rate of which is relatively high in sewage. This study focused on the UF membrane process for the reclaimed water treatment process, and investigated the removal performance in NV type GI and GII in the UF membrane process by performing coagulation and sedimentation as the pre-treatment process in a pilot-plant by considering the concentration fluctuation of the influent. The removal ratio of GI and GII by the UF membrane process alone was 3.3 ± 0.7 Log in GI and 3.6 ± 1.0 Log in GII, and no clear difference in the removal ratio by NV species type was observed. The removal ratio of NV GII was increased by about 0.6 Log on average (4.2 ± 1.1 Log) compared with the UF membrane process only when the coagulation and sedimentation process were conducted as pre-treatment. However, there was no significant difference in the removal of NV GI by conducting the coagulation and sedimentation process.

Study on interactions between suspended matter and biofouling formed by treated sewage.

Heat exchangers used for treated sewage energy recovery usually suffer from the composite fouling problem, which seriously impairs the heat transfer efficiency. Treated sewage heat exchanger composite fouling is mostly composed of biofouling and is notably affected by interactions between the biofouling and suspended matter. Experiments were performed using simulated treated sewage and two kinds of simulated suspended matter, silicon dioxide particles and polyamide filaments, to model the interactions. Different flow velocities, particle sizes and concentrations were tested with their influences presented by the fouling wet weight changes. Empirical equation and threshold were developed based on the results to predict whether the suspended matter promotes or impedes fouling growth. The results indicate that proper control of the flow velocities, particle sizes and concentrations of suspended matter using empirical equation and threshold can inhibit fouling by reducing unwanted positive interactions and promoting beneficial negative interactions. The filament interactions were analysed and the unique attachment mechanisms of filaments were discussed for the first time.

Characterisation of a diverse range of circular replication-associated protein encoding DNA viruses recovered from a sewage treatment oxidation pond.

Our knowledge of circular replication-associated protein encoding single-stranded (CRESS) DNA virus diversity has increased dramatically in recent years, largely due to advances in high-throughput sequencing technologies. These viruses are apparently major virome components in most terrestrial and aquatic environments and it is therefore of interest to determine their diversity at the interfaces between these environments. Treated sewage water is a particularly interesting interface between terrestrial and aquatic viromes in that it is directly pumped into waterways and is likely to contain virus populations that have been strongly impacted by humans. We used a combination of high-throughput sequencing, full genome PCR amplification, cloning and Sanger sequencing to investigate the diversity of CRESS DNA viruses present in a sewage oxidation pond. Using this approach, we recovered 50 putatively complete novel CRESS viral genomes (it remains possible that some are components of multipartite viral genomes) and 11 putatively sub-genome-length circular DNA molecules which may be either defective genomes or components of multipartite genomes. Thirteen of the genomes have bidirectional genome organisations and share similar conserved replication-associated protein (Rep) motifs to those of the gemycircularviruses: a group that in turn is most closely related to the geminiviruses. The remaining 37 viral genomes share very low degrees of Rep similarity to those of all other known CRESS DNA viruses. This number of highly divergent CRESS DNA virus genomes within a single sewage treatment pond further reinforces the notion that there likely exist hundreds of completely unknown genus/family level CRESS DNA virus groupings.

Concentrated microalgae cultivation in treated sewage by membrane photobioreactor operated in batch flow mode.

This study investigated the microalgae biomass production and nutrients removal efficiency from treated sewage by newly developed membrane photobioreactor in which Chlorella vulgaris was cultured in batch flow mode. Its performance was compared with conventional photobioreactor. The results show that the volumetric microalgae productivity was 39.93 and 10.36 mg L(-1)d(-1) in membrane photobioreactor and conventional photobioreactor, respectively. The nutrients removal rate in membrane photobioreactor was 4.13 mg N L(-1)d(-1) and 0.43 mg P L(-1)d(-1), which was obviously higher than that in conventional photobioreactor (0.59 mg N L(-1)d(-1) and 0.08 mg P L(-1)d(-1)). The better performance of membrane photobioreactor was due to the submerged membrane module in the reactor which acted as a solid-liquid separator and thereby enabled the reactor to operate with higher supply flow rate of cultivation medium. Moreover, in the outflow stage of the membrane photobioreactor, the microalgae culture liquor in the reactor could be further concentrated.