Detection of Multiple Human Viruses, including Mpox, Using a Wastewater Surveillance Approach in Brazil.

Sewage surveillance can be used as an effective complementary tool for detecting pathogens in local communities, providing insights into emerging threats and aiding in the monitoring of outbreaks. In this study using qPCR and whole genomic sewage surveillance, we detected the Mpox virus along with other viruses, in municipal and hospital wastewaters in Belo Horizonte, Brazil over a 9-month period (from July 2022 until March 2023). MPXV DNA detection rates varied in our study, with 19.6% (11 out of 56 samples) detected through the hybrid capture method of whole-genome sequencing and 20% (12 out of 60 samples) through qPCR. In hospital wastewaters, the detection rate was higher, at 40% (12 out of 30 samples) compared to 13.3% (4 out of 30 samples) in municipal wastewaters. This variation could be attributed to the relatively low number of MPXV cases reported in the city, which ranged from 106 to 341 cases during the study period, and the dilution effects, given that each of the two wastewater treatment plants (WWTP) investigated serves approximately 1.1 million inhabitants. Additionally, nine other virus families were identified in both hospitals and municipal wastewaters, including Adenoviridade, Astroviridae, Caliciviridae, Picornaviridade, Polyomaviridae, Coronaviridae (which includes SARS-CoV-2), Herspesviridae, Papillomaviridae and Flaviviridae (notably including Dengue). These findings underscore the potential of genomic sewage surveillance as a robust public health tool for monitoring a wide range of viruses circulating in both community and hospitals environments, including MPXV.

Influence of ozonation and UV/H2O2 on the genotoxicity of secondary wastewater effluents.

The implementation of novel wastewater treatment technologies, including Advanced Oxidation Processes (AOPs) such as ozonation and ultraviolet radiation (UV) combined with hydrogen peroxide (H2O2), can be a promising strategy for enhancing the quality of these effluents. However, during effluent oxidation AOPs may produce toxic compounds that can compromise the water reuse and the receiving water body. Given this possibility, the aim of this study was to evaluate the genotoxic potential of secondary effluents from two different Wastewater Treatment Plants (WWTP) that were subjected to ozonation or UV/H2O2 for periods of 20 (T1) and 40 (T2) minutes. The genotoxic potential was carried out with the Comet assay (for clastogenic damage) and the Micronucleus assay (for clastogenic and aneugenic damage) in HepG2/C3A cell culture (metabolizing cell line). The results of the comet assay revealed a significant increase in tail intensity in the Municipal WWTP (dry period) effluents treated with UV/H2O2 (T1 and T2). MN occurrence was noted across all treatments in both Pilot and Municipal WWTP (dry period) effluents, whereas nuclear buds (NBs) were noted for all Pilot WWTP treatments and UV/H2O2 treatments of Municipal WWTP (dry period). Moreover, the UV/H2O2 (T1) treatment of Municipal WWTP (dry period) exhibited a noteworthy incidence of multiple alterations per cell (MN + NBs). These findings imply that UV/H2O2 treatment demonstrates higher genotoxic potential compared to ozonation. Furthermore, seasonal variations can have an impact on the genotoxicity of the samples. Results of the study emphasize the importance of conducting genotoxicological tests using human cell cultures, such as HepG2/C3A, to assess the final effluent quality from WWTP before its discharge or reuse. This precaution is essential to safeguard the integrity of the receiving water body and, by extension, the biotic components it contains.

A COMPRENHESIVE ECO-EFFICIENCY ANALYSIS OF WASTEWATER TREATMENT PLANTS: ESTIMATION OF OPTIMAL OPERATIONAL COSTS AND GREENHOUSE GAS EMISSIONS.

The transition to a neutral carbon and sustainable urban water cycle requires improving eco-efficiency in wastewater treatment processes. To support decision-making based on eco-efficiency evaluations, reliable estimations are fundamental. In this study, the eco-efficiency of a sample of 109 WWTPs was evaluated using efficiency analysis tree method. It combines machine learning and linear programming techniques and therefore, overcomes overfitting limitations of non-parametric methods used by past research on this topic. Results from the case study revealed that optimal costs and greenhouse gas emissions depend on the quantity of organic matter and suspended solids removed from wastewater. The estimated average eco-efficiency is 0.373 which involves that the assessed WWTPs could save 0.32 €/m3 and 0.11 kg of CO2 equivalent/m3. Moreover, only 4 out of 109 WWTPs are identified as eco-efficient which implies that the majority of the evaluated facilities can achieve substantial savings in operational costs and greenhouse gas emissions.

A comprehensive assessment of energy efficiency of wastewater treatment plants: An efficiency analysis tree approach.

Wastewater treatment plants (WWTPs) are energy intensive facilities. Controlling energy use in WWTPs could bring substantial benefits to people and environment. Understanding how energy efficient the wastewater treatment process is and what drives efficiency would allow treating wastewater in a more sustainable way. In this study, we employed the efficiency analysis trees approach, that combines machine learning and linear programming techniques, to estimate energy efficiency of wastewater treatment process. The findings indicated that considerable energy inefficiency among WWTPs in Chile existed. The mean energy efficiency was 0.287 suggesting that energy use should cut reduce by 71.3 % to treat the same volume of wastewater. This was equivalent to a reduction in energy use by 0.40 kWh/m3 on average. Moreover, only 4 out of 203 assessed WWTPs (1.97 %) were identified as energy efficient. It was also found that the age of treatment plant and type of secondary technology played an important role in explaining energy efficiency variations among WWTPs.

Ecotoxicological assessment of estuarine surface waters receiving treated and untreated sanitary wastewater.

Pollution from sewage discharge is one of the most critical environmental problems worldwide, e.g., in Brazil, where basic sanitation is still scarce. As pollution can affect biomes, especially estuaries where intensive ecological and human activities occur, has caused widespread concern. This work aimed to study the water quality of the Jundiaí/Potengi Estuary (JPE) in an area close to the discharge of treated and untreated wastewater for 18 months. Physicochemical and microbiological parameters were measured and integrated using the Water Quality Index of the Canadian Council of Ministers of the Environment. Ecotoxicological tests were performed with Brazilian endemic organisms to assess the impact of water pollution on biota. A generalized linear regression model was applied to understand the effects of water quality on ecotoxicological responses. Concentrations of metals, dissolved oxygen, total ammonia nitrogen, nitrate, and thermotolerant coliforms did not comply with Brazilian environmental regulations. A significant increase in the mortality rate of Mysidopsis juniae and Nitocra sp. and a significant decrease in the reproductive rate of Nitocra sp. indicated the most affected areas related to the discharge of treated and untreated wastewater. Only 10% of the samples from sites without direct wastewater impact showed a toxic response in at least one organism. Both water quality and sampling sites were statistical predictors of ecotoxicological response, describing not only the pollutant load but also the type of effluent. This study demonstrated the degradation of the environmental quality of the JPE, particularly due to the discharge of sanitary wastewater, and highlights the importance of protection and remediation measures to preserve this protected area.

Low biological phosphorus removal from effluents treated by slow sand filters.

The legislation for environment protection requires strict controls of the wastewater releasing in water bodies. The wastewater treatment plants (WWTP) have been used for organic matter degradation; however, the residual total phosphorus (TP) removal has not been efficient. TP and nitrogen present in wastewater are associated to eutrophication of water bodies and algae growth. Therefore, this study discusses the efficiency of phosphorus removal by a slow filter (SF), complementary to a WWTP and the microbial community involved. The results showed that the use of SF, with or without macrophytes, is not suitable to remove TP. Spatial variation in microbial communities distributed in three distinct zones was identified in the SF. Proteobacteria, Bacteroidetes, Chloroflexi and Firmicutes covered the hydrolytic and fermentative bacteria. The acetogenesis, nitrification, and denitrification, as well as the removal of phosphorus from the effluent, were performed by representatives affiliated to different groups. Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria among these, Dokdonella sp., Frateuria sp., Comamonas sp., Diaphorobacter sp., Nitrosospira sp., Ferruginibacter sp., Flavobacterium sp., and the uncultured OD1 were the most abundant bacteria in the SF. The low efficiency for TP removing from SF effluents can be explained by the low abundance of phosphorus accumulating organisms (PAOs), with the association of the low concentration of biodegradable organic matter in the inlet effluent. Therefore, the alternative to using SF as a complement to WWTPs, as recommended by some Brazilian environmental agencies, did not prove to be viable and new approaches must be evaluated. KEY POINTS: • The phosphorus removal was performed by a slow filter system in a WWTP but obtained a low efficiency. • Microbial spatial variation was distributed into distinct zones from slow filter. • Low abundance of PAOs was observed due to the low availability of organic matter.

Efficient removal of antibiotics from water resources is a public health priority: a critical assessment of the efficacy of some remediation strategies for antibiotics in water.

This review discusses the fundamental principles and mechanism of antibiotic removal from water of some commonly applied treatment techniques including chlorination, ozonation, UV-irradiation, Fenton processes, photocatalysis, electrochemical-oxidation, plasma, biochar, anaerobicdigestion, activated carbon and nanomaterials. Some experimental shortfalls identified by researchers such as certain characteristics of degradation agent applied and the strategies explored to override the identified limitations are briefly discussed. Depending on interactions of a range of factors including the type of antibiotic compound, operational parameters applied such as pH, temperature and treatment time, among other factors, all reviewed techniques can eliminate or reduce the levels of antibiotic compounds in water to varying extents. Some of the reviewed techniques such as anaerobic digestion generally require longer treatment times (up to 360, 193 and 170 days, according to some studies), while others such as photocatalysis achieved degradation within short contact time (within a minimum of 30, but up to 60, 240, 300 and 1880 minutes, in some cases). For some treatment techniques such as ozonation and Fenton, it is apparent that subjecting compounds to longer treatment times may improve elimination efficiency, whereas for some other techniques such as nanotechnology, application of longer treatment time generally meant comparatively minimal elimination efficiency. Based on the findings of experimental studies summarized, it is apparent that operational parameters such as pH and treatment time, while critical, do not exert sole or primary influence on the elimination percentage(s) achieved. Elimination efficiency achieved rather seems to be due more to the force of a combination of several factors.

Climatological and Epidemiological Conditions Are Important Factors Related to the Abundance of blaKPC and Other Antibiotic Resistance Genes (ARGs) in Wastewater Treatment Plants and Their Effluents, in an Endemic Country.

Several physicochemical and season factors have been related to the abundance of antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs), considered hotspots of bacterial resistance. However, few studies on the subject have been carried out in tropical countries endemic for resistance mechanisms such as blaKPC. In this study, the occurrence of ARGs, particularly blaKPC, was determined throughout a WWTP, and the factors related to their abundance were explored. In 2017, wastewater samples were taken from a WWTP in Colombia every 15 days for 6 months, and a total of 44 samples were analyzed by quantitative real-time PCR. sul1, sul2, blaKPC, and ermB were found to be the most prevalent ARGs. A low average reduction of the absolute abundance ARGs in effluent with respect to influent was observed, as well as a greater absolute abundance of ARGs in the WWTP effluent in the rainy season. Factors such as temperature, pH, oxygen, total organic carbon (TOC), chemical oxygen demand (COD), and precipitation were significantly correlated with the absolute abundance of several of the ARGs evaluated. A generalized linear mixed-effects model analysis showed that dissolved oxygen and precipitation in the sampling day were important factors related to the absolute concentration of blaKPC over time. In conclusion, the abundance of ARGs in the WWTP could be influenced by endemic conditions and physicochemical and climatological parameters. Therefore, it is necessary to continuously monitor clinical relevant genes in WWTPs from different global regions, even more so in low-income countries where sewage treatment is limited.

High Resistance of a Sludge Enriched with Nitrogen-Fixing Bacteria to Ammonium Salts and Its Potential as a Biofertilizer.

The increasing use of chemical fertilizers causes the loss of natural biological nitrogen fixation in soils, water eutrophication and emits more than 300 Mton CO2 per year. It also limits the success of external bacterial inoculation in the soil. Nitrogen fixing bacteria can be inhibited by the presence of ammonia as its presence can inhibit biological nitrogen fixation. Two aerobic sludges from wastewater treatment plants (WWTP) were exposed to high ammonium salts concentrations (>450 mg L-1 and >2 dS m-1). Microbial analysis after treatment through 16S pyrosequencing showed the presence of Fluviicola sp. (17.70%), a genus of the Clostridiaceae family (11.17%), and Azospirillum sp. (10.42%), which were present at the beginning with lower abundance. Denaturing gradient gel electrophoresis (DGGE) analysis based on nifH genes did not show changes in the nitrogen-fixing population. Nitrogen-Fixing Bacteria (NFB) were identified and associated with other microorganisms involved in the nitrogen cycle, presumably for survival at extreme conditions. The potential use of aerobic sludges enriched with NFB is proposed as an alternative to chemical fertilizer as this bacteria could supplement nitrogen to the plant showing competitive results with chemical fertilization.

Metagenomic analysis of urban wastewater resistome and mobilome: A support for antimicrobial resistance surveillance in an endemic country.

In developing countries, where high levels of antimicrobial resistance are observed in hospitals, the surveillance of this phenomenon in wastewater treatment plants (WWTPs) and the environment is very limited, especially using cutting-edge culture-independent methods. In this study, the composition of bacterial communities, the resistome and mobilome (the pool of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), respectively) at a WWTP were determined using shotgun metagenomics and culture-based approaches. Wastewater samples were collected at four sampling points of a WWTP in Antioquia, Colombia. A total of 24 metagenomes were analyzed. Specifically, there were marked differences in bacterial community composition, resistome, and mobilome, according to the WWTP sampling points. Bacterial families of clinical importance such as Moraxellaceae, Aeromonadaceae, and Enterobacteriaceae were mainly detected in the WWTP influent and effluent samples. Genes encoding resistance to macrolide-lincosamide-streptogramin, ß-lactams, and those conferring multidrug resistance (e.g., acrB, adeG, and mexD) were the most abundant. Moreover, some clinically important ARGs such as blaKPC-2 and blaCTX-M, and others not reported locally, such as blaTEM-196, blaGES-23, blaOXA-10, mcr-3, and mcr-5 were frequently detected. Co-occurrence network analyses indicated a significant association of ARGs such as blaOXA-58 and blaKPC genes with Aeromonadaceae and Enterobacteriaceae. Among the markers of MGEs, intI1 and ISCR8 were the most frequently detected. Altogether, this work reveals the importance of shotgun metagenomics and culture-based approaches in antimicrobial resistance studies. The findings also support that WWTPs are hotspots for antimicrobial resistance, whose analysis constitutes a powerful tool to predict the impact of antimicrobial resistance in a population.

Quantitative microbial risk assessment of SARS-CoV-2 for workers in wastewater treatment plants.

Faecal-oral transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is yet to be validated, but it is a critical issue and additional research is needed to elucidate the risks of the novel coronavirus in sanitation systems. This is the first study that investigates the potential health risks of SARS-CoV-2 in sewage to wastewater treatment plant (WWTP) workers. A quantitative microbial risk assessment (QMRA) is applied for three COVID-19 scenarios (moderate, aggressive and extreme) to study the effects of different stages of the pandemic in terms of percentage of infected population on the probability of infection to WWTP workers. A dose-response model for SARS-CoV-1 (as a surrogate pathogen) is assumed in the QMRA for SARS-CoV-2 using an exponential model with k = 4.1 × 102. Literature data are incorporated to inform assumptions for calculating the viral load, develop the model, and derive a tolerable infection risk. Results reveal that estimates of viral RNA in sewage at the entrance of WWTPs ranged from 4.14 × 101 to 5.23 × 103 GC·mL-1 (viable virus concentration from 0.04 to 5.23 PFU·mL-1, respectively). In addition, estimated risks for the aggressive and extreme scenarios (2.6 × 10-3 and 1.3 × 10-2, respectively) were likely to be above the derived tolerable infection risk for SARS-CoV-2 of 5.5 × 10-4 pppy, thus reinforcing the concern of sewage systems as a possible transmission pathway of SARS-CoV-2. These findings are helpful as an early health warning tool and in prioritizing upcoming risk management strategies, such as Emergency Response Plans (ERPs) for water and sanitation operators during the COVID-19 and future pandemics.

High frequency of gram-negative bacilli harboring blaKPC-2 in the different stages of wastewater treatment plant: A successful mechanism of resistance to carbapenems outside the hospital settings.

Wastewater treatment plants (WWTPs) are considered to be a reservoir and a source of bacterial resistance. Worryingly, the presence of carbapenem-resistant Gram-negative bacilli (CRGNB) in WWTPs has recently been reported, but there are still many research gaps regarding its emergence and impact. The distribution of CRGNB in the different stages of a WWTP in Colombia and the relationship between the physicochemical factors involved with their presence are described in this paper. Additionally, given the impact on public health, the CRGNB detected were compared with isolates previously found in hospital patients. Residual water samples were taken from five different stages of a WWTP between January and July 2017. A total of 390 GNB were isolated, and a significant frequency of CRGNB harboring blaKPC-2 (38.2%, n = 149/390) was detected, of which 57% were Enterobacteriaceae, 41.6% Aeromonadaceae, and 1.3% Pseudomonadaceae. The Enterobacteriaceae were more frequent in the raw effluent compared to the Aeromonadaceae, which in turn were more prevalent in the recycled activated sludge and final effluent. Environmental variables such as pH, oxygen, chemical oxygen demand, and temperature were significantly correlated with the quantification of carbapenem-resistant Enterobacteriaceae (CRE) at specific points in the WWTP. Interestingly, isolated K. pneumoniae harboring blaKPC-2 from the WWTPs were diverse and did not relate genetically to the hospital strains with which they were compared. In conclusion, these results confirm the worrying scenario of the dissemination and persistence of emerging contaminants such as CRGNB harboring blaKPC-2, and reinforce the need to establish strategies aimed at containing this problem using multifocal interventions.

Bacteria from industrial waste: potential producers of polyhydroxyalkanoates (PHAs) in Manizales, Colombia.

Polymers are currently used in the industry as raw material, yet they are rapidly eliminated and largely contaminate the environment. To address this issue, there is a special interest in biodegradable polymers, namely, polyhydroxyalkanoates (PHAs), produced by microorganisms. This study identifies PHA-producing bacteria from two industrial wastewaters of Manizales, Colombia. The samples were cultured in mineral salt medium with glucose as the carbon source in the presence of Nile red stain. The fluorescent colonies were independently transferred to another medium and assessed through fluorescence microscopy with Nile blue stain. The fluorescent strains under Nile blue staining were purified in Nutrient Agar, and their morphological and microbiological characteristics were determined. The bacteria positive for red-orange fluorescence were purified in Nutrient Agar medium, and molecular analyses were performed by PCR amplification of a 650-bp fragment of the 16S ribosomal DNA gene. The bacteria were also assessed in terms of PHA production. We confirmed the identity of 12 out of 14 PHA-positive strains, which belonged to the following genera: Bacillus, Lactococcus, Citrobacter, Enterobacter, and Acinetobacter. Five of the isolates (Enterobacter cloacae, Enterobacter sp., Enterobacter ludwigii, Bacillus thuringiensis, and Bacillus safensis) are promising strains for PHA production, with production values ranging from 0.360 to 0.9960 g/L. Bacteria that produce more than 0.3 g/L are considered useful for the industrial manufacture of bioplastic. We recommend performing large-scale studies on these strains to assess their use for the industrial production of biopolymers, allowing to generate high-impact bioconversion processes of industrial interest.

High clonal diversity of multidrug-resistant and extended spectrum beta-lactamase-producing Escherichia coli in a wastewater treatment plant.

Increasing beta-lactam resistance has led to the exploration of different places, such as wastewater treatment plants (WWTPs) which have been considered to be reservoirs and sources of bacterial resistance. This work aims to determine the presence of beta-lactamase-producing-Enterobacteriaceae in different points of a WWTP in Colombia. Six samplings were carried out in 2017 in the raw influent, aeration tanks, recycled sludge and final effluent of a WWTP. The beta-lactamase-producing-Enterobacteriaceae were detected and identified using phenotypic and molecular methods. Of the 353 isolates included, 28.3% corresponded to enterobacteria. The most frequent microorganisms were Escherichia coli (83%), Citrobacter freundii (11%) and Enterobacter cloacae complex (4%). The 97% of enterobacteriaceae had at least one beta-lactamase, and the most prevalent were the blaTEM (43.8%) and blaCTX-M-1group (35.8%) which were detected specially in recycled sludge and final effluent sample points. High percentage of multidrug resistance (to beta-lactams and non-beta-lactam antibiotics) was detected in E. coli (63.2%). Additionally, the typing by PFGE and MLST showed high genotypic diversity and the presence of the successful ST131 clone, globally spread. This work highlights the strong role of E. coli as a vector for the dissemination of resistance and the beta-lactamases in aquatic environments.

Noroviruses in raw sewage, secondary effluents and reclaimed water produced by sand-anthracite filters and membrane bioreactor/reverse osmosis system.

The importance of noroviruses (NoVs) in the epidemiology of waterborne diseases has increased globally in the last decades. The present study aimed to monitor genogroup I and II noroviruses in different treatment stages of four wastewater treatment plants (WWTPs) in the metropolitan São Paulo. WWTPs consist of secondary (activated sludge) and tertiary treatments (coagulation, sand-anthracite filters, membrane bioreactor (MBR)/reverse osmosis (RO) and chlorination). Raw sewage (500mL) and treated effluents (1L) were concentrated by celite and reclaimed water (40L) by hollow-fiber ultrafiltration system. Quantitative (qPCR) and nested PCR with nucleotide sequencing were used for quantification and molecular characterization. NoVs were widely distributed in raw wastewater samples (83.3%-100% NoV GI and 91.6%-100% NoV GII) and viral loads varied from 3.8 to 6.66log10gcL-1 for NoV GI and 3.8 to 7.3log10gcL-1 for NoV GII. Mean virus removal efficiencies obtained for activated sludge processes ranged from 0.3 to 0.8 log10 for NoV GI and 0.4 to 1.4 log10 for NoV GII. NoVs were not detected in the reuse water produced by MBR/RO system, while sand-anthracite filters resulted in a NoV GI and GII decay of 1.1-1.6 log10 and 0.7-1.6 log10, respectively. A variety of genotypes (GI.2, GI.3a, GI.3b, GI.5, GII.1, GII.4 Sydney 2012, GII.5, GII.6, GII.17) was observed, with a predominance of GI.2 and GII.17 in the different genogroups. These results corroborate with recent data about the entry and dissemination of the emerging genotype GII.P17-GII.17 Kawasaki 2014 in the country, and may indicate a change in the epidemiological patterns of norovirus strains circulation in this region. This is the first large-scale study to evaluate burden and genotypes of noroviruses in WWTPs in Brazil, providing a rapid diagnosis of viruses circulating in the population.

Estimaciones del consumo de drogas ilícitas derivadas del análisis de aguas residuales: Una revisión crítica / Illicit drug consumption estimations derived from wastewater analysis: A critical review

Resumen La medición cuantitativa de drogas ilícitas en aguas residuales puede proporcionar información objetiva sobre los patrones y tendencias del uso de psicotrópicos en la comunidad. Se analizó la presencia de psicoactivos en plantas de tratamiento para aguas residuales y en el medio ambiente por epidemiología de alcantarillado, mediante una revisión sistemática de 2008-2017 por medio de los descriptores controlados: "drogas ilícitas", "plantas de tratamiento de aguas residuales" y "ambiente" hecha en las bases de datos Medline vía PubMed, SciELO, BVS vía LILACS y Google Académico. La búsqueda arrojó 439 estudios y fueron seleccionados 34 estudios para análisis. Los años de publicación variaron, siendo 2016 el de mayor de publicaciones (diez), seguido de 2014 y 2012 (cinco), 2017, 2013, 2010 (tres), 2011 (dos), 2015, 2009 y 2008 (uno cada uno). Los resultados mostraron la presencia de indicadores químicos de las drogas clásicas cocaína y cannabis. La investigación sobre la presencia de drogas ilícitas, particularmente como compuestos activos en el medio ambiente, es vital para mejorar el conocimiento de los tipos consumidos, su prevalencia; siendo capaz desplegar para analizar la exposición y el impacto de estos compuestos en el medio ambiente y la salud pública. Los resultados de esta revisión han resaltado que la epidemiología basada en aguas residuales es un enfoque útil y poderoso.

Abstract Quantitative measurement of illicit drugs in wastewater can provide objective information on community drug use patterns and trends. The presence of illicit drugs in treatment plants for wastewater and in the environment was analyzed by sewege epidemiology. It is a systematic review from 2008-2017 by means of the controlled descriptors: "illicit drugs", "wastewater treatment plants" and "environment" performed on Medline databases via PubMed, SciELO, BVS via LILACS and Google Scholar. The search yielded 439 studies. 34 studies were selected for analysis. The years of publication varied, with 2016 being the highest of publications (ten), followed by 2014 and 2012 (five), 2017, 2013, 2010 (three), 2011 (two), 2015, 2009 and 2008 (one each). The results showed the presence of chemical indicators of the classic drugs cocaine and cannabis. Research on the presence of illicit drugs, particularly as active compounds in the environment, are vital to improve knowledge of the types consumed, its occurrence; being able to unfold to analyze the exposure and the impact of these compounds in the environment and public health. The results of this revision have highlighted that wastewater-based epidemiology is a useful and powerful approach.

'An investigation into the occurrence and removal of pharmaceuticals in Colombian wastewater'.

In this work, the presence of 20 pharmaceuticals in wastewater from Colombia is investigated. Several widely consumed compounds have been detected in wastewater samples from different origins and geographical areas in Colombia. The studied pharmaceuticals included antibiotics, analgesics and anti-inflammatories, cholesterol lowering statin drugs, lipid regulators, and anti-depressants. The investigated samples were urban wastewater collected during one whole week before (influent) and after treatment (effluent) in the wastewater treatment plants (WWTPs) of Bogotá and Medellin. Raw wastewater from the Hospital of Tumaco and from the city of Florencia were also collected. Analyses performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that most of the target analytes were present in all the wastewater samples. The highest concentrations (up to 50 µg/L) corresponded to acetaminophen, but several antibiotics, such as azithromycin, ciprofloxacin and norfloxacin, and antihypertensive drugs, such as losartan and valsartan, were commonly present in influent wastewater (IWW) at levels above 1 µg/L. Moreover, the treatment applied in WWTPs seemed to not efficiently remove the compounds under study, because most pharmaceuticals were also present in effluent wastewater (EWW) at concentrations close to those of the IWW. Special emphasis was made in this work on the quality of data reported, performing a detailed study of quality control (QC) samples. The analytical approach used -direct injection of 5-fold diluted samples without any additional treatment - is simpler and faster than the commonly applied solid phase extraction (SPE). The use of 12 isotope-labelled internal standards ensured the satisfactory correction of matrix effects for the corresponding analytes. For the remaining 8 compounds, no drastic matrix effects were observed, and only four compounds (cloxacillin, doxycycline, losartan, tetracycline) presented QC recoveries near or slightly below 60%, revealing ionization suppression, particularly in the IWW. Data on the occurrence of pharmaceuticals reported in this paper are the basis for current studies that aim to develop efficient systems for the degradation/removal of these compounds from the aquatic environment.

Study of pharmaceuticals in surface and wastewater from Cuernavaca, Morelos, Mexico: Occurrence and environmental risk assessment.

The present work describes the first known study to date on the occurrence of pharmaceuticals in surface water and wastewater of Cuernavaca, the capital of the state of Morelos (México). Selected pharmaceuticals (a total of 35) were extracted from the collected water samples with a generic solid phase extraction (SPE) protocol and determined in the sample extracts by means of high-performance liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). A screening level risk assessment combining the measured environmental concentrations (MECs) with dose-response data based on predicted no-effect concentrations (PNECs) was also applied to estimate Hazard Quotients (HQs) for the pharmaceuticals detected in the investigated area. A total of twelve pharmaceuticals were found in the water samples analyzed, with detection frequencies above 78% and in most cases of 100%. Overall, the most abundant pharmaceuticals in surface water were the analgesic and anti-inflammatory drugs naproxen (732-4880ng/L), acetaminophen (354-4460ng/L), and diclofenac (258-1398ng/L), and the lipid regulator bezafibrate (286-2100ng/L). On the contrary, other compounds like the ß-blocker atenolol and the psychiatric drug carbamazepine were found at only a few ng or tens of ng per liter in the Apatlaco River. Despite the fact that some of the most abundant compounds showed good removal (>97%) during wastewater treatment, concentrations downstream the WWTP were only slightly lower than upstream. This indicates the existence of additional inputs of untreated wastewater into the river. Based on the obtained HQ-values, the concentrations of ibuprofen, sulfamethoxazole, diclofenac and naproxen present in the river could pose a high toxicity risk for the aquatic ecosystem. These findings highlight these pharmaceuticals as relevant organic contaminants in the area of study and the need to further monitor them in order to adopt appropriate measures to safeguard the ecosystem, and eventually human health.

Ciliated protozoa community of a combined UASB-activated sludge system in southeastern Brazil.

The aims of the present study were (1) to evaluate the abundance and taxonomic composition of ciliated protozoa in the activated sludge of a full-scale combined anaerobic-aerobic system operating in a tropical country and (2) to study the relationship between the effluent quality, the physicochemical variables, and the ciliates present in the operating system. The total ciliate fauna of the activated sludge of the Piçarrão Wastewater Treatment Plant (Piçarrão WWTP) was composed of 36 morphospecies belonging to 33 genera. These included 21 species observed in the activated sludge samples on the day of collection and 15 species found in cultures. The activated sludge of the Piçarrão WWTP contained a diversified ciliate community composed mainly of indicator organisms. The most frequently occurring morphospecies were Aspidisca cicada, Vorticella spp., Gastronauta aloisi, Acineria uncinata, and Epistylis plicatilis complex. These results showed that satisfactory operating conditions prevailed at the Piçarrão WWTP. In the combined UASB-activated sludge system, the presence of Aspidisca cicada suggests the occurrence of denitrification in the process while the presence of Acineria uncinata and G. alosi indicates the removal of carbonaceous organic matter.

Bacterial communities involved in sulfur transformations in wastewater treatment plants.

The main sulfate-reducing (SRB) and sulfur-oxidizing bacteria (SOB) in six wastewater treatment plants (WWTPs) located at southern Brazil were described based on high-throughput sequencing of the 16S rDNA. Specific taxa of SRB and SOB were correlated with some abiotic factors, such as the source of the wastewater, oxygen content, sample type, and physical chemical attributes of these WWTPs. When the 22 families of SRB and SOB were clustered together, the samples presented a striking distribution, demonstrating grouping patterns according to the sample type. For SOB, the most abundant families were Spirochaetaceae, Chromatiaceae, Helicobacteriaceae, Rhodospirillaceae, and Neisseriaceae, whereas, for SRB, were Syntrophaceae, Desulfobacteraceae, Nitrospiraceae, and Desulfovibriaceae. The structure and composition of the major families related to the sulfur cycle were also influenced by six chemical attributes (sulfur, potassium, zinc, manganese, phosphorus, and nitrogen). Sulfur was the chemical attribute that most influenced the variation of bacterial communities in the WWTPs (λ = 0.14, p = 0.008). The OTUs affiliated to Syntrophus showed the highest response to the increase of total sulfur. All these findings can contribute to improve the understanding in relation to the sulfur-oxidizing and sulfate-reducing communities in WWTPs aiming to reduce H2S emissions.