The applicability of Beckman PK7300 fully automatic blood group instrument for TPPA detection / 中国输血杂志

【Objective】 To investigate the applicability of Beckman PK7300 for TPPA testing on anti-TP reactive specimens from blood donors. 【Methods】 1) The TPPA test using Beckman PK7300 (abbreviated as instrument method) had been established and the performance was verificated by calculating the total compliance rate, positive compliance rate and negative compliance rate as compared with the manual method. The repeatability of this instrument method was also evaluated. 2) The applicability of the instrument method was evaluated by examing 555 TP-reactive samples for 6 consecutive days, so as to analyze the readable reading rate, agglutination strength distribution and other control methods. 【Results】 1) The total, positive, and negative compliance rates of TPPA detection by both instrumental and manual methods were 100% (kappa value =1). The SPC value of samples, read manually as " + + ", was less than or equal to 3 by Beckman PK7300; the SPC value of samples, read manually as " -", was greater than or equal to 20 by Beckman PK7300. The two methods were well consistent. The instrument method was repeated for 12 times for the same samples, and the accuracy rate was 100% (12 / 12), with good repeatability.2) The results of the TPPA test in 555 anti-TP reactivity specimens showed an overall readable rate of 99.82%(554/555). The SPC values of the negative and unsensitized particles of TPPA were distributed on both sides of the determination value without crossover. The control and monitor thoughout the test were carried out automatically by the instrument. 【Conclusion】 The TPPA test conducted by the Beckman PK7300 fully automatic blood group instrument is suitable for the confirmatory experiment of anti-TP reactive specimens in blood center laboratories, which could realize the automation and standardization of TPPA detection.

Study on blood donor deferral strategy by analyzing anti-TP test results / 中国输血杂志

【Objective】 To discuss the reliability and applicability of the current blood deferral strategy concerning anti-TPreactive blood donors (by ELISA). 【Methods】 TPPA confirmatory test was performed on the samples routinely detected by two different anti-TP ELISA reagents(reagent 1 and reagent 2), and the test data of dual reagent reactive and one reagent reactive blood donors were analyzed to determine the possibility of true positivity. 【Results】 1 624 anti-TP reactive samples(by ELISA) were collected, among which 1 467 were dual reagent reactive, 77 were reagent 1 reactive, and 80 were reagent 2 reactive. TPPA results showed that the positive predictive value (PPV) of dual reactive samples was 85.48%. Samples with high S/CO value (reagent 1≥13 and/or reagent 2 >17) were more likely to be true positive, with the PPV at 98.56% (reagent 1) and 99.13% (reagent 2), respectively, which were significantly higher than that when the S/CO value was≥1. Among the samples reactive to one reagent, 2 were confirmed positive in reagent 1 and 3 in reagent 2, with the PPV at 2.60% and 3.75% respectively, and had no correlation with high S/CO value. 【Conclusion】 Dual-reagent reactive donors with high S/CO value showed high possibility of true positivity, therefore should be deferred. TPPA test is helpful to identify true positivity in one-reagent reactive donors. Confirmatory test and follow-up should be a supplement to the current blood donor deferral strategy to ensure blood safety.

Effect of anti-TP0136 antibodies on the progression of lesions in an infected rabbit model.

The effect of anti-TP0136 antibodies on the progression of syphilis is poorly understood. This study aimed to investigate the effect of anti-TP0136 antibodies on the progression of lesions in an infected rabbit model. Intramuscular injection of rTP0136 into rabbits in the immunized group (n = 4) elicited high titers of anti-TP0136 antibodies, and rabbits were then challenged with 105T. pallidum per site along their back. Lesion development was observed, and the injection sites were biopsied for tp0574 mRNA and histological analyses every week until the wound healed. The rabbits in the control group were injected with normal saline instead of rTP0136. Viable T. pallidum in the challenged rabbits was assessed with rabbit infectivity tests. The lesions in the immunized group took longer to heal than those in the control group (42 d vs. 28 d, P < 0.001) and had markedly higher levels of total cellular infiltrates. The mRNA level of tp0574 in the immunized group was significantly higher than that in the control group (P < 0.05). Viable T. pallidum was detected in rabbit lymph nodes in both the immunized and control groups. Our study showed that high titers of anti-TP0136 antibodies promoted the infiltration of inflammatory cells into local lesions and intensified tissue damage, thus delaying wound healing, and had no protective effect on the occurrence of syphilis in the rabbit model.

Analysis on results of treponema pallidum antibody detection among 50 011 inpatients / 国际检验医学杂志

Objective To analyze the results of anti‐Treponema pallidum antibody (anti‐TP) in the inpatients to provide the ba‐sis for the prevention and control of syphilis .Methods The results of anti‐TP test among 50 011 patients in our hospital from Jan . 2013 to Nov .2015 were retrospectively analyzed .Results Of 50 011 detected inpatients ,1 360 cases were anti‐TP positive with the total anti‐TP positive rate of 2 .72% ,in which the male positive rate was 3 .22% (719 cases)and the female positive rate was 2 .32%(641 cases) ,the difference was statistically significant (P< 0 .05) .The neonates ,populations aged ≥80 years old and 40 - < 60 years had higher anti‐TP positive rate ,which were 3 .67% ,3 .62% and 3 .24% respectively .The inpatients with anti‐TP positive were mainly concentrated in the surgical departments ,totally 916 cases(67 .35% ) ,in which the general surgery department had the highest anti‐TP positive detection rate(20 .51% ) ,while 444 cases(32 .65% ) of anti‐TP positive were in the non‐surgical depart‐ments .Among the inpatients with anti‐TP positive ,520 cases(38 .24% ) had the positive titer in the serum TRUST .Conclusion Syphilis infection situation is serious ,the routine anti‐TP detection in inpatients has an important significance for preventing nosoco‐mial infection and reducing the medical dispute .

Analyze of the False Positive Rate of Serum Anti Treponema Pallidum Specific Antibody of 26 707 Cases Detected by Chemiluminescence Method / 现代检验医学杂志

Objective To detect serum anti-Treponema pallidum specific antibody of 26 707 cases by Abbott I2000SR auto-matic chemiluminescent microparticle immunoassay analyzer,and treponema pallidum particle agglutination assay (TPPA) was regarded as a standard reference method which was used to detect anti-Treponema pallidum specific antibody.To analyze the false positive rate of Abbott I2000SR according to the TPPA.Methods Collected 26 707 serums from inpatients and outpatients of the hospital during September 1,2013 to March 5,2014.The subjects were asked to fasting conditions taking venous blood 3 ml,3 000 r/min centrifugal 10 min utes after the separation of serum,detected the Anti-TP by CMIA (Ab-bott I2000SR)and the TPPA testing,analyzed test results by statistical methods.Results There were 52 cases detected by I2000SR whose S/CO values of 26 707 cases of serum Treponema pallidum specific antibodies were 1 to 2,of which 9 cases were verified positive by TPPA,and the positive rate was 17.31%.There were 26 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were 2 to 3,of which 9 cases were verified positive by TPPA,and the posi-tive rate was 34.62%.There were 26 cases detected by I2000SR whose S/CO values of Treponema pallidum specific anti-bodies were 3 to 5,of which 9 cases were verified positive by TPPA,and the positive rate was 34.62%.There were 25 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were 5 to 7,of which 11 cases were veri-fied positive by TPPA,and the positive rate was 44%.There were 25 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were 7 to 10,of which 17 cases were verified positive by TPPA,and the positive rate was 68%.There were 28 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were 10to 13,of which 24 cases were verified positive by TPPA,and the positive rate was 85.71%.There were 23 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were 13 to 17,of which 20 cases were verified posi-tive by TPPA,and the positive rate was 86.96%.There were 24 cases detected by I2000SR whose S/CO values of Trepone-ma pallidum specific antibodies were 17 to 21,of which 22 cases were verified positive by TPPA,and the positive rate was 91.67%.There were 29 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were 21 to 26,of which 28 cases were verified positive by TPPA,and the positive rate was 96.55%.There were 104 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were above 26,of which 104 cases were verified posi-tive by TPPA,and the positive rate was 100%.The total number of positive cases were 364,of which 254 were positive ca-ses,the positive rate was 69.78%.False positive rate was 0.42% and positive predictive value was 69.78%.Conclusion Abbott I2000SR automated chemiluminescent microparticle immunoassay analyzer has the feature of automated detection, closed reagents,simple operation,speed,and more accurate results and so on.Although high sensitivity but its results have false positive,so cannot diagnose based on the results of Abbott I2000SR,and need use of the TPPA to test and corroborate.

Detection of four infection indicators in 28165 patients before transfusion and surgery / 中国感染控制杂志

Objective To investigate the detection results of HBsAg,anti-HCV,anti-Treponema pallidum anti-body (anti-TP )and anti-HIV in patients before transfusion and surgery. Methods Four infection indicators of 28 165 patients were detected by enzyme-linked immunosorbent assay from June 2011 to May 2012,results were an-alyzed statistically.Results Of 28 165 patients,total positive rate was 12.15% (n= 3 422),the positive rate of HB-sAg,anti-HCV,anti-TP and anti-HIV was 8.69% (n= 2 447),1.31% (n= 368),2.07% (n= 583),and 0.09% (n=24)respectively. The positive rate of HBsAg,anti-HCV and anti-HIV in male was higher than female (χ2 was 36.64,28.95,and 4.82,respectively,allP<0.05).In different age groups,positive rate of all indicators in<20 age group was lowest,while positive rates of HBsAg,anti-HCV,and anti-HIV were highest in 20-39 and 40-59 age groups,anti-TP was highest in ≥60 age group.Conclusion Detection of bloodborne pathogens before transfusion and surgery is helpful for realizing infection status of patients before transfusion and surgery.

Analysis of Detection Indicators of Blood Donor’s Transfusion Infection in Wuxi Area from 2 0 0 5 to 2 0 1 2 / 现代检验医学杂志

Objective To understand the Wuxi area index detection of infectious blood donors in the blood situation,in order to take targeted measures to block the blood-borne diseases.Methods From 2005 to 2012,Wuxi area (not including county, district)blood donation blood samples 329 254 (person)copies of the test results were retrospectively analyzed.Results 8 years,Wuxi unpaid donors HBsAg,anti-HCV,anti-HIV,and anti-TP four tested positive rates were 0.587%,0.452%, 0.212% and 0.509%,the total positive rate was 1.76%.Different years of anti-HCV,anti-HIV and anti-TP positive rate difference was statistically significant (χ2= 40.74~138.24,P<0.01).From 2011 to 2012 HBsAg positive rate was signifi-cantly increased,and compared with the previous year,difference was statistically significant (χ2=47.56,52.34,P<0.01), while four the number of positive indicators of infection in different sex,age and occupation with different proportions of dis-tribution.Conclusion The positive rate of most indicators declined year by year,but there was still an upward trend in indi-vidual proj ects.Should further improve the different groups of scientific blood donation advocacy work to ensure blood safe-ty.

Test Data Analysis of Blood Donors in Beijing 2001-2008 / 中华医院感染学杂志

OBJECTIVE To provide current tendency of viral transmission by test-negative blood components changing among blood donors and to improve the safety of blood for transfusion.METHODS The test data of 1 608 816 blood donors in Beijing Red Cross Blood Center from 2001 to 2008 were analyzed.RESULTS Before voluntary blood donation,the positive rate of anti-HCV was 0.16%,that of anti-HIV was 0.005% and that of anti-TP was 0.15%.After voluntary blood donation,positive rate of these components increased,there were 0.45%,0.017% and 0.48%,respectively,but the positive rate of HBsAg and ALT was decreased.After voluntary blood donation,the positive rate of HBsAg,anti-HCV,anti-HIV and anti-TP were elevated,that of ALT was decreased in greatly.CONCLUSIONS As the increasing risk of viral transmission disease,it is more important for blood safety to screen volunteer blood donors.