EVALUATION OF A COMBINATION OF TILETAMINE-ZOLAZEPAM, MEDETOMIDINE, AND AZAPERONE WITH NASAL OXYGEN SUPPLEMENTATION FOR THE IMMOBILIZATION OF CAPTIVE CHACOAN PECCARIES (CATAGONUS WAGNERI) IN THE CHACO REGION OF PARAGUAY.

A combination of tiletamine-zolazepam, medetomidine, and azaperone was used to immobilize captive Chacoan peccaries (Catagonus wagneri) for health assessments and biological sample collection at the Centro Chaqueño para la Conservación e Investigación (CCCI) in the Paraguayan Chaco during July in 2017 and 2018. In total, 83 peccaries kept in 0.25-1.50 hectare enclosures were immobilized via dart-administered anesthetic. Mean animal weight was 33.89±3.74 kg (standard deviation; n=77). The mean intramuscular (IM) anesthetic drug and dosages were 0.03±0.00 mg/kg of medetomidine, 0.91±0.10 mg/kg of Zoletil 50 (tiletamine-zolazepam), and 0.30±0.03 mg/kg azaperone. The mean time to recumbency after darting was 6.07±2.65 min. The mean time to reach the anesthetic plane postdarting was 10.00±2.00 min. Muscle relaxation was adequate to allow minor veterinary procedures. A mean dosage of 0.15±0.02 mg/kg of atipamezole was given IM to reverse the medetomidine. Recoveries were smooth and animals were standing by 59.17±30.18 min postreversal. Full recovery and release back to enclosures occurred 90±30 min postreversal. A single dose of this drug combination provided adequate anesthesia for 88% of adult Chacoan peccaries; 12% needed a supplemental dose of tiletamine-zolazepam because of failure to receive the full dose from the anesthetic dart. Sex and age did not impact the dosage required to achieve immobilization. Confinement during recovery from anesthesia is required with this protocol. Aside from mild hypoxemia, no adverse effects from anesthesia were observed. However, oxygen supplementation as a part of this protocol is recommended to support circulatory and respiratory capacity.

Analgesic and sedative evaluation of two protocols in swine underwent orchiectomy / Avaliação analgésica e sedativa de dois protocolos em suínos submetidos à orquiectomia

Introduction: Sedation and analgesia are essential in chemical restraint in pigs for various procedures. Analgesia allows theanimal to be free of the deleterious effects of pain, which are related to stress and weight loss. In this sense, the aim of this studywas evaluate two sedation protocols in pigs undergoing elective orchiectomy.Materials, Methods and Results: There were used 22 pigs with three months old, with an average weight of 19.05 ± 2.46 kg.The food was withheld for 12 h and water for 6 h. After fasting period, they were mask-induced with isofl urane, for surgicalpreparation and than they were recovered from anesthesia. After 30 min, the animals were allocated in two groups: ketamine/midazolam /Tramadol (CMT, n = 11) who received 5 mg kg-1 of ketamine, 1 mg kg,-1 of midazolam and 4 mg kg-1 of tramadol,and azaperone/tramadol (AT, n = 11) which received 6 mg kg-1 of azaperone and 4 mg kg-1 of tramadol, both treatments administered intramuscularly. The parameters evaluated were: heart rate (HR) through the stethoscope, respiratory frequency (f) by themovement of the rib cage, systolic blood pressure (SBP), mean blood pressure (MBP) and diastolic blood pressure (DBP) trougha catheter in femoral artery, rectal temperature (RT) with a digital thermometer, arterial blood gas analysis, with an blood gasanalyzer, and blood count and biochemical profi le. The moments of evaluation were: baseline, and 10, 20, 30 and 40 min aftertreatments, corresponding to M0, M1, M2, M3 and M4, respectively. Score of sedation, muscle relaxation, response to stimuli,time to sternal recumbency, time to deambulation, time to full recovery and quality of recovery were also evaluated. The meansbetween groups in the same time were analyzed by Student’s t test and the means between times, in the same group by analysisof variance followed by Student Newmann Keuls test (P  0.05). Non parametrical data were analyzed with Mann Whitney rank...

Analgesic and sedative evaluation of two protocols in swine underwent orchiectomy / Avaliação analgésica e sedativa de dois protocolos em suínos submetidos à orquiectomia

Introduction: Sedation and analgesia are essential in chemical restraint in pigs for various procedures. Analgesia allows theanimal to be free of the deleterious effects of pain, which are related to stress and weight loss. In this sense, the aim of this studywas evaluate two sedation protocols in pigs undergoing elective orchiectomy.Materials, Methods and Results: There were used 22 pigs with three months old, with an average weight of 19.05 ± 2.46 kg.The food was withheld for 12 h and water for 6 h. After fasting period, they were mask-induced with isofl urane, for surgicalpreparation and than they were recovered from anesthesia. After 30 min, the animals were allocated in two groups: ketamine/midazolam /Tramadol (CMT, n = 11) who received 5 mg kg-1 of ketamine, 1 mg kg,-1 of midazolam and 4 mg kg-1 of tramadol,and azaperone/tramadol (AT, n = 11) which received 6 mg kg-1 of azaperone and 4 mg kg-1 of tramadol, both treatments administered intramuscularly. The parameters evaluated were: heart rate (HR) through the stethoscope, respiratory frequency (f) by themovement of the rib cage, systolic blood pressure (SBP), mean blood pressure (MBP) and diastolic blood pressure (DBP) trougha catheter in femoral artery, rectal temperature (RT) with a digital thermometer, arterial blood gas analysis, with an blood gasanalyzer, and blood count and biochemical profi le. The moments of evaluation were: baseline, and 10, 20, 30 and 40 min aftertreatments, corresponding to M0, M1, M2, M3 and M4, respectively. Score of sedation, muscle relaxation, response to stimuli,time to sternal recumbency, time to deambulation, time to full recovery and quality of recovery were also evaluated. The meansbetween groups in the same time were analyzed by Students t test and the means between times, in the same group by analysisof variance followed by Student Newmann Keuls test (P  0.05). Non parametrical data were analyzed with Mann Whitney rank...(AU)

Azaperone e sua associação com xilazina ou dexmedetomidina em suínos / Azaperone and its association with xilazina or dexmedetomidina in swines

O estudo objetivou avaliar o efeito sedativo do azaperone e de sua associação com a xilazina ou dexmedetomidina na espécie suína, assim como verificar a possibilidade de o agente butirofenônico contrabalançar os efeitos causados pelos agonistas α2-adrenérgicos nos parâmetros cardiovasculares. Foram estudados 18 suínos hígidos da linhagem Dambread X MS 50, de 50 dias de idade, pesando 17,3kg (±1,7). Todos os animais foram submetidos a anestesia com isofluorano para instrumentação necessária ao protocolo experimental e, 30 minutos após a recuperação anestésicas, os parâmetros basais foram mensurados e os animais alocados aleatoriamente em três grupos de seis animais cada: GA (Azaperone 2mg kg-1 + Cloreto de sódio 0,5ml - IM), GAD (Azaperone 2mg kg-1 + Dexmedetomidina 3µg kg-1 - IM), GAX (Azaperone 2mg kg-1 + Xilazina 2mg kg-1 - IM). Os parâmetros foram novamente avaliados aos 15, 30, 45 e 60 minutos após administração dos fármacos correspondentes aos grupos do estudo. A frequência cardíaca teve seus valores reduzidos em todos os grupos, porém essa redução foi maior no GAX. Durante o estudo não foi observado efeito bifásico sobre a pressão arterial, com hipertensão seguida de hipotensão. O GAX apresentou redução de PaO2 e aumento de PaCO2, assim como observou-se melhor efeito sedativo nesse grupo. Os resultados permitem concluir que a associação de azaperone com xilazina promoveu melhor sedação e miorrelaxamento e menor resposta a estímulos.

The aim of this study was to evaluate the sedative effects of azaperone and its association with xylazine or dexmedetomidine in swine, as well as verifying the possibility of the butyrophenone agent to counterbalance the effects caused by α2-adreneceptor agonists on the cardiovascular and hemodynamic parameters. For this, eighteen healthy swines of the Dambread X MS 50 lineage aged 50 days-old, weighing around 17.3kg (±1.7) were used. All animals were submitted an isoflurane anesthesia by face mask throughout the period of instrumentation. Basal parameters were measured 30 minutes after recovering from general anesthesia. All swines were randomly assigned into three groups of six animals each: AG (azaperone 2mg kg-1 + sodium chloride 0.5ml - IM), ADG (azaperone 2mg kg-1 + dexmedetomidine 3µg kg-1 - IM) and AXG (azaperone 2mg kg-1 + xylazine 2mg kg-1 - IM). Parameters were again measured at the following times: 15, 30, 45 and 60 minutes after administrating the corresponding drugs to each group. The heart rate had its values reduced in all groups; however this reduction was more significative in AXG. During the study was not observed a biphasic effect over the arterial pressure with an initial increase followed by a gradual decrease. AXG presented reduction of PaO2 and an increase in PaCO2 as well as a better sedative effect. The results allow to conclude that the association of azaperone with xylazine promoted a better tranquilization and muscular relaxation.

Contenção farmacológica e anestesia do queixada (Tayassu pecari Link, 1795), pela associação de azaperone, tiletamina zolazepam, romifidina e atropina, com protocolos calculados por extrapolação alométrica interespecífica / Chemical restraint and anesthesia of the white-lipped-peccary (Tayassu pecari Link, 1795), with the combination of azaperone, romifidine, tiletamine, zolazepam, and atropine with doses calculated by allometric scaling

Onze queixadas (Tayassu pecari), pertencentes ao plantel do zoológico do Parque Municipal Danilo Galafassi (Cascavel, Paraná, Brasil), foram anestesiados pela associação de azaperone, romifidina, tiletamina, zolazepam e sulfato de atropina. As doses foram estabelecidas por meio de extrapolação alométrica interespecífica, usando como modelo as doses indicadas para animais domésticos. Para romifidina usou-se como modelo a dose de 0,10 mg/kg, indicada para um cavalo de 500,00 Kg; para um cão de 10,00 Kg; para azaperone usou-se como modelo a dose de 1,00 mg/kg, indicada para um suíno de 100,00 kg; e para atropina usou-se como modelo a dose de 0,05 mg/kg, indicada para um cão de 10 kg. Conhecendo-se os pesos dos indivíduos, as doses calculadas das drogas foram acondicionadas em um mesmo dardo e administradas à distância por meio de zarabatana. Os animais foram avaliados quanto à perda da reação postural de endireitamento, início da anestesia, retorno da consciência, retorno da reação postural de endireitamento e retorno à ambulação normal. A cada 10 minutos após a indução da anestesia, foram monitorizados os parâmetros fisiológicos temperatura retal, freqüência cardíaca, freqüência respiratória e saturação parcial de oxigênio. Nos mesmos momentos, avaliou-se a qualidade da contenção farmacológica (pelo nível de miorrelaxamento) e da anestesia (pelas reações a estímulos dolorosos), sendo conferidos os conceitos A (excelente), B (bom) e C (ruim). No 100° minuto realizou-se a administração intravenosa de ioimbina, avaliando-se seus efeitos na recuperação da anestesia. Concluiu-se que o protocolo testado é eficaz para a contenção farmacológica e anestesia de queixadas(AU)

Eleven White-lipped-peccaries (Tayassu pecari) belongig to the zoo of the City Park Danilo Galafassi (Cascavel, Paraná, Brazil) were anesthetized by the association of azaperone, romifidine, tiletamine, zolazepam, and atropine sulfate. The doses were established by allometric scaling, using as models the doses indicated for domestic animals: a) for romifidine the model was the dose or 0,10 mg/kg, indicated for a 500,00 kg horse, b) for the tiletamine + zolazepam association the model was the dose of 5,0 mg/kg, indicated for a 10,00 kg dog, c0 for azaperone the model was the dose of 1,00 mg/kg, indicated for a 100,00 kg pig, d) for atropine the model was the dose of 0,05 mg/kg, indicated for a 10 kg dog. Knowing the individuals” weights, the calculated doses of the drugs were mixed in the same dart and remotely administered by a blwgun. There were observed loss of righting reflex, beginning of anesthesia, return of conscience, return of righting reflex, and return to normal ambulation. Physiologic parameters (rectal temperature, heart rate, respiratory rate, and partial saturation of oxygen) were monitored every 10 minutes after the induction of the anesthesia. In the same moments there were evaluated the quality of the chemical restraint (by the muscle relaxation level, and the anesthesia (by the reactions to painful stimuli), being given the concepts A (excellent), B (good) and C (bad). In the 100th minute it was administered intravenous yohimbine and its effects on the recovering were evaluated. It was concluded that that the tested protocol is effective for chemical restraint and anesthesia of the white-lipped-peccary(AU)

Contenção farmacológica e anestesia do queixada (Tayassu pecari Link, 1795), pela associação de azaperone, tiletamina zolazepam, romifidina e atropina, com protocolos calculados por extrapolação alométrica interespecífica / Chemical restraint and anesthesia of the white-lipped-peccary (Tayassu pecari Link, 1795), with the combination of azaperone, romifidine, tiletamine, zolazepam, and atropine with doses calculated by allometric scaling

Onze queixadas (Tayassu pecari), pertencentes ao plantel do zoológico do Parque Municipal Danilo Galafassi (Cascavel, Paraná, Brasil), foram anestesiados pela associação de azaperone, romifidina, tiletamina, zolazepam e sulfato de atropina. As doses foram estabelecidas por meio de extrapolação alométrica interespecífica, usando como modelo as doses indicadas para animais domésticos. Para romifidina usou-se como modelo a dose de 0,10 mg/kg, indicada para um cavalo de 500,00 Kg; para um cão de 10,00 Kg; para azaperone usou-se como modelo a dose de 1,00 mg/kg, indicada para um suíno de 100,00 kg; e para atropina usou-se como modelo a dose de 0,05 mg/kg, indicada para um cão de 10 kg. Conhecendo-se os pesos dos indivíduos, as doses calculadas das drogas foram acondicionadas em um mesmo dardo e administradas à distância por meio de zarabatana. Os animais foram avaliados quanto à perda da reação postural de endireitamento, início da anestesia, retorno da consciência, retorno da reação postural de endireitamento e retorno à ambulação normal. A cada 10 minutos após a indução da anestesia, foram monitorizados os parâmetros fisiológicos temperatura retal, freqüência cardíaca, freqüência respiratória e saturação parcial de oxigênio. Nos mesmos momentos, avaliou-se a qualidade da contenção farmacológica (pelo nível de miorrelaxamento) e da anestesia (pelas reações a estímulos dolorosos), sendo conferidos os conceitos A (excelente), B (bom) e C (ruim). No 100° minuto realizou-se a administração intravenosa de ioimbina, avaliando-se seus efeitos na recuperação da anestesia. Concluiu-se que o protocolo testado é eficaz para a contenção farmacológica e anestesia de queixadas

Eleven White-lipped-peccaries (Tayassu pecari) belongig to the zoo of the City Park Danilo Galafassi (Cascavel, Paraná, Brazil) were anesthetized by the association of azaperone, romifidine, tiletamine, zolazepam, and atropine sulfate. The doses were established by allometric scaling, using as models the doses indicated for domestic animals: a) for romifidine the model was the dose or 0,10 mg/kg, indicated for a 500,00 kg horse, b) for the tiletamine + zolazepam association the model was the dose of 5,0 mg/kg, indicated for a 10,00 kg dog, c0 for azaperone the model was the dose of 1,00 mg/kg, indicated for a 100,00 kg pig, d) for atropine the model was the dose of 0,05 mg/kg, indicated for a 10 kg dog. Knowing the individuals” weights, the calculated doses of the drugs were mixed in the same dart and remotely administered by a blwgun. There were observed loss of righting reflex, beginning of anesthesia, return of conscience, return of righting reflex, and return to normal ambulation. Physiologic parameters (rectal temperature, heart rate, respiratory rate, and partial saturation of oxygen) were monitored every 10 minutes after the induction of the anesthesia. In the same moments there were evaluated the quality of the chemical restraint (by the muscle relaxation level, and the anesthesia (by the reactions to painful stimuli), being given the concepts A (excellent), B (good) and C (bad). In the 100th minute it was administered intravenous yohimbine and its effects on the recovering were evaluated. It was concluded that that the tested protocol is effective for chemical restraint and anesthesia of the white-lipped-peccary

Azaperone e sua associação com xilazina ou dexmedetomidina em suínos

The aim of this study was to evaluate the sedative effects of azaperone and its association with xylazine or dexmedetomidine in swine, as well as verifying the possibility of the butyrophenone agent to counterbalance the effects caused by 2-adreneceptor agonists on the cardiovascular and hemodynamic parameters. For this, eighteen healthy swines of the Dambread X MS 50 lineage aged 50 days-old, weighing around 17.3kg (±1.7) were used. All animals were submitted an isoflurane anesthesia by face mask throughout the period of instrumentation. Basal parameters were measured 30 minutes after recovering from general anesthesia. All swines were randomly assigned into three groups of six animals each: AG (azaperone 2mg kg-1 + sodium chloride 0.5ml - IM), ADG (azaperone 2mg kg-1 + dexmedetomidine 3µg kg-1 - IM) and AXG (azaperone 2mg kg-1 + xylazine 2mg kg-1 - IM). Parameters were again measured at the following times: 15, 30, 45 and 60 minutes after administrating the corresponding drugs to each group. The heart rate had its values reduced in all groups; however this reduction was more significative in AXG. During the study was not observed a biphasic effect over the arterial pressure with an initial increase followed by a gradual decrease. AXG presented reduction of PaO2 and an increase in PaCO2 as well as a better sedative effect. The results allow to conclude that the association of azaperone with xylazine promoted a better tranquilization and muscular relaxation.

O estudo objetivou avaliar o efeito sedativo do azaperone e de sua associação com a xilazina ou dexmedetomidina na espécie suína, assim como verificar a possibilidade de o agente butirofenônico contrabalançar os efeitos causados pelos agonistas 2-adrenérgicos nos parâmetros cardiovasculares. Foram estudados 18 suínos hígidos da linhagem Dambread X MS 50, de 50 dias de idade, pesando 17,3kg (±1,7). Todos os animais foram submetidos a anestesia com isofluorano para instrumentação necessária ao protocolo experimental e, 30 minutos após a recuperação anestésicas, os parâmetros basais foram mensurados e os animais alocados aleatoriamente em três grupos de seis animais cada: GA (Azaperone 2mg kg-1 + Cloreto de sódio 0,5ml - IM), GAD (Azaperone 2mg kg-1 + Dexmedetomidina 3µg kg-1 - IM), GAX (Azaperone 2mg kg-1 + Xilazina 2mg kg-1 - IM). Os parâmetros foram novamente avaliados aos 15, 30, 45 e 60 minutos após administração dos fármacos correspondentes aos grupos do estudo. A frequência cardíaca teve seus valores reduzidos em todos os grupos, porém essa redução foi maior no GAX. Durante o estudo não foi observado efeito bifásico sobre a pressão arterial, com hipertensão seguida de hipotensão. O GAX apresentou redução de PaO2 e aumento de PaCO2, assim como observou-se melhor efeito sedativo nesse grupo. Os resultados permitem concluir que a associação de azaperone com xilazina promoveu melhor sedação e miorrelaxamento e menor resposta a estímulos.

Azaperone e sua associação com xilazina ou dexmedetomidina em suínos

The aim of this study was to evaluate the sedative effects of azaperone and its association with xylazine or dexmedetomidine in swine, as well as verifying the possibility of the butyrophenone agent to counterbalance the effects caused by 2-adreneceptor agonists on the cardiovascular and hemodynamic parameters. For this, eighteen healthy swines of the Dambread X MS 50 lineage aged 50 days-old, weighing around 17.3kg (±1.7) were used. All animals were submitted an isoflurane anesthesia by face mask throughout the period of instrumentation. Basal parameters were measured 30 minutes after recovering from general anesthesia. All swines were randomly assigned into three groups of six animals each: AG (azaperone 2mg kg-1 + sodium chloride 0.5ml - IM), ADG (azaperone 2mg kg-1 + dexmedetomidine 3µg kg-1 - IM) and AXG (azaperone 2mg kg-1 + xylazine 2mg kg-1 - IM). Parameters were again measured at the following times: 15, 30, 45 and 60 minutes after administrating the corresponding drugs to each group. The heart rate had its values reduced in all groups; however this reduction was more significative in AXG. During the study was not observed a biphasic effect over the arterial pressure with an initial increase followed by a gradual decrease. AXG presented reduction of PaO2 and an increase in PaCO2 as well as a better sedative effect. The results allow to conclude that the association of azaperone with xylazine promoted a better tranquilization and muscular relaxation.

O estudo objetivou avaliar o efeito sedativo do azaperone e de sua associação com a xilazina ou dexmedetomidina na espécie suína, assim como verificar a possibilidade de o agente butirofenônico contrabalançar os efeitos causados pelos agonistas 2-adrenérgicos nos parâmetros cardiovasculares. Foram estudados 18 suínos hígidos da linhagem Dambread X MS 50, de 50 dias de idade, pesando 17,3kg (±1,7). Todos os animais foram submetidos a anestesia com isofluorano para instrumentação necessária ao protocolo experimental e, 30 minutos após a recuperação anestésicas, os parâmetros basais foram mensurados e os animais alocados aleatoriamente em três grupos de seis animais cada: GA (Azaperone 2mg kg-1 + Cloreto de sódio 0,5ml - IM), GAD (Azaperone 2mg kg-1 + Dexmedetomidina 3µg kg-1 - IM), GAX (Azaperone 2mg kg-1 + Xilazina 2mg kg-1 - IM). Os parâmetros foram novamente avaliados aos 15, 30, 45 e 60 minutos após administração dos fármacos correspondentes aos grupos do estudo. A frequência cardíaca teve seus valores reduzidos em todos os grupos, porém essa redução foi maior no GAX. Durante o estudo não foi observado efeito bifásico sobre a pressão arterial, com hipertensão seguida de hipotensão. O GAX apresentou redução de PaO2 e aumento de PaCO2, assim como observou-se melhor efeito sedativo nesse grupo. Os resultados permitem concluir que a associação de azaperone com xilazina promoveu melhor sedação e miorrelaxamento e menor resposta a estímulos.

Comparative study of the sedative and antinociceptive effects of levomepromazine, azaperone and midazolam in laboratory animals / Estudo comparativo dos efeitos sedativos e antinociceptivos de levomepromazina, azaperone e midazolam em animais de laboratório

The sedative and antinociceptive effects of levomepromazine, azaperone and midazolam were studied in rats and mice using three behavior evaluation methods. Both exploratory behavior and spontaneous locomotor activity were significantly diminished in a spontaneous locomotor activity test in open field when using levomepromazine and azaperone. However, the azaperone effects were short lived in comparison to levomepromazine effects. Midazolam caused reduction in exploratory activity with no effect in spontaneous locomotion. When assessing the antinociceptive effect in the tail flick reflex latency test after infliction of a pain stimulus in rats, tested drugs did not show any antinociceptive effect. The drugs studied were able to abolish the writhing reflex in mice when compared to control. Levomepromazine, azaperone and midazolam, at the doses were able to inhibit the exploratory behavior in rats, proving their sedative effect. Regarding the antinociceptive effects for visceral pain, these drugs were able to block contortions in mice.(AU)

Os efeitos sedativos e antinociceptivos da levomepromazina, azaperone e midazolam foram avaliados utilizando-se três testes de comportamento em ratos e camundongos. No teste da atividade locomotora espontânea em campo aberto observou-se que tanto o comportamento exploratório como a atividade locomotora espontânea foram significativamente diminuídos quando se utilizou levomepromazina e azaperone. O efeito causado pelo azaperone foi menos prolongado quando comparado ao da levomepromazina. O midazolam causou diminuição do comportamento exploratório sem alterar a atividade locomotora espontânea. Quando se avaliou o efeito antinociceptivo por meio da latência para o reflexo da retirada da cauda em ratos após estímulo doloroso, as drogas não apresentaram nenhum efeito antinociceptivo observável. No teste das contorções em camundongos, os fármacos foram capazes de abolir as contorções quando comparados ao efeito do grupo-controle. Levomepromazina, azaperone e midazolam nas doses utilizadas foram capazes de inibir o comportamento exploratório de ratos, comprovando seus efeitos sedativos. Com relação aos efeitos antinociceptivos para dor visceral, eles foram capazes de inibir as contorções.(AU)

Comparative study of the sedative and antinociceptive effects of levomepromazine, azaperone and midazolam in laboratory animals

The sedative and antinociceptive effects of levomepromazine, azaperone and midazolam were studied in rats and mice using three behavior evaluation methods. Both exploratory behavior and spontaneous locomotor activity were significantly diminished in a spontaneous locomotor activity test in open field when using levomepromazine and azaperone. However, the azaperone effects were short lived in comparison to levomepromazine effects. Midazolam caused reduction in exploratory activity with no effect in spontaneous locomotion. When assessing the antinociceptive effect in the tail flick reflex latency test after infliction of a pain stimulus in rats, tested drugs did not show any antinociceptive effect. The drugs studied were able to abolish the writhing reflex in mice when compared to control. Levomepromazine, azaperone and midazolam, at the doses were able to inhibit the exploratory behavior in rats, proving their sedative effect. Regarding the antinociceptive effects for visceral pain, these drugs were able to block contortions in mice.

Os efeitos sedativos e antinociceptivos da levomepromazina, azaperone e midazolam foram avaliados utilizando-se três testes de comportamento em ratos e camundongos. No teste da atividade locomotora espontânea em campo aberto observou-se que tanto o comportamento exploratório como a atividade locomotora espontânea foram significativamente diminuídos quando se utilizou levomepromazina e azaperone. O efeito causado pelo azaperone foi menos prolongado quando comparado ao da levomepromazina. O midazolam causou diminuição do comportamento exploratório sem alterar a atividade locomotora espontânea. Quando se avaliou o efeito antinociceptivo por meio da latência para o reflexo da retirada da cauda em ratos após estímulo doloroso, as drogas não apresentaram nenhum efeito antinociceptivo observável. No teste das contorções em camundongos, os fármacos foram capazes de abolir as contorções quando comparados ao efeito do grupo-controle. Levomepromazina, azaperone e midazolam nas doses utilizadas foram capazes de inibir o comportamento exploratório de ratos, comprovando seus efeitos sedativos. Com relação aos efeitos antinociceptivos para dor visceral, eles foram capazes de inibir as contorções.