Design, Development and Testing of a Monitoring System for the Study of Proton Exchange Fuel Cells and Stacks.

This article is about the design, development and validation of a new monitoring architecture for individual cells and stacks to facilitate the study of proton exchange fuel cells. The system consists of four main elements: input signals, signal processing boards, analogue-to-digital converters (ADCs) and a master terminal unit (MTU). The latter integrates a high-level graphic user interface (GUI) software developed by National Instruments LABVIEW, while the ADCs are based on three digital acquisition units (DAQs). Graphs showing the temperature, currents and voltages in individual cells as well as stacks are integrated for ease of reference. The system validation was carried out both in static and dynamic modes of operation using a Ballard Nexa 1.2 kW fuel cell fed by a hydrogen cylinder, with a Prodigit 32612 electronic load at the output. The system was able to measure the voltage distributions of individual cells, and temperatures at different equidistant points of the stack both with and without an external load, validating its use as an indispensable tool for the study and characterization of these systems.

Methylpiperidinium Iodides as Novel Antagonists for α7 Nicotinic Acetylcholine Receptors.

The α7 nicotinic acetylcholine receptor (nAChR) is expressed in neuronal and non-neuronal cells and is involved in several physiopathological processes, and is thus an important drug target. We have designed and synthesized novel piperidine derivatives as α7 nAChR antagonists. Thus, we describe here a new series of 1-[2-(4-alkoxy-phenoxy-ethyl)]piperidines and 1-[2-(4-alkyloxy-phenoxy-ethyl)]-1-methylpiperidinium iodides (compounds 11a-11c and 12a-12c), and their actions on α7 nAChRs. The pharmacological activity of these compounds was studied in rat CA1 hippocampal interneurons by using the whole-cell voltage-clamp technique. Inhibition of the choline-induced current was less for 11a-11c than for the methylpiperidinium iodides 12a-12c and depended on the length of the aliphatic chain. Those compounds showing strong effects were studied further using molecular docking and molecular dynamics simulations. The strongest and non-voltage dependent antagonism was shown by 12a, which could establish cation-π interactions with the principal (+)-side and van der Waals interactions with the complementary (-)-side in the α7 nAChRs. Furthermore, compound 11a forms hydrogen bonds with residue Q115 of the complementary (-)-side through water molecules without forming cation-π interactions. Our findings have led to the establishment of a new family of antagonists that interact with the agonist binding cavity of the α7 nAChR, which represent a promising new class of compounds for the treatment of pathologies where these receptors need to be negatively modulated, including neuropsychiatric disorders as well as different types of cancer.