RESUMO
AIM:To optimize the purification of Acanthopanax senticosus Harms root extract based on the rodent with anticerebral ischemia. METHODS:We identified the effect of D-101 macroporous resin adsorption,polyamide adsorption and n-butanol extraction on cerebral ischemia-reperfusion injure model rat and behead-mouth(opening) model mouse in the treatment of aqueous acanthopanax root extract. RESULTS:Aqueous acanthopanax root extract,which was purfied by using D-101 macroporous resin,had advantage over that were treated with polyamide adsorption and n-butanol extraction.Eleutheroside content in the root extract showed the positive correlation with anticerebral ischemia. CONCLUSION:D-101 macroporous resin adsorption is a good purification method for acanthopanax root extract.