Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integrated Pseudomonas sp. JY-Q with surface-co-displayed PETase and MHETase.

The waste pollution problem caused by polyethylene terephthalate (PET) plastics poses a huge threat to the environment and human health. As plasticizers, Phthalate esters (PAEs) are widely used in PET production and become combined pollutants with PET. Synthetic biology make it possible to construct engineered cells for microbial degradation of combined pollutants of PET and PAEs. PET hydroxylase (PETase) and monohydroxyethyl terephthalate hydroxylase (MHETase) isolated from Ideonella sakaiensis 201-F6 exhibit the capability to depolymerize PET. However, PET cannot enter cells, thus enzymatic degradation or cell surface displaying technology of PET hydrolase are the potential strategies. In this study, Pseudomonas sp. JY-Q was selected as a chassis strain, which exhibits robust stress tolerance. First, a truncated endogenous outer membrane protein cOmpA and its variant Signal (OprF)-cOmpA were selected as anchor motifs for exogenous protein to display on the cell surface. These anchor motifs were fused at the N-terminal of PET hydrolase and MHETase and transformed into Pseudomonas sp. JY-Q, the mutant strains successfully display the enzymes on cell surface, after verification by green fluorescent protein labeling and indirect immunofluorescence assay. The resultant strains also showed the catalytic activity of co-displaying PETase and MHETase for PET biodegradation. Then, the cell surface displaying PET degradation module was introduced to a JY-Q strain which genome was integrated with PAEs degrading enzymes and exhibited PAEs degradation ability. The resultant strain JY-Q-R1-R4-SFM-TPH have the ability of degradation PET and PAEs simultaneously. This study provided a promising strain resource for PET and PAEs pollution control.

A comparative study on the toxic effects of lead pollution and nanoplastic-lead mixed pollution on red drum and their detoxification strategies.

Nanoplastics and heavy metals pose various adverse effects on marine organisms. However, the combined toxicity of nanoplastics and lead pollution to marine fish is not fully understood. This study investigates the toxic effects and detoxification strategies of lead pollution (p07) compared to nanoplastic-lead mixed pollution (m07) in red drum during exposure and recovery phases. Under m07 pollution, the maximum lead content in muscle was 22.61 mg/kg, which was significantly higher than the 15.82 mg/kg observed under p07 pollution. This finding demonstrated that nanoplastics significantly enhance lead accumulation, leading to more severe toxic effects on red drum. Histological analyses revealed that lipid droplets in the liver and epithelial lifting in the gills were the primary lesion types. During the exposure periods, red drum primarily detoxified lead through cellular renewal and the removal of damaged proteins under p07 pollution. Conversely, under m07 pollution, detoxification relied on cellular senescence, apoptosis, endocytosis, and the removal of damaged proteins. In the recovery phases, red drum predominantly recovered through cell proliferation and antioxidant responses under p07 pollution. Under m07 pollution, the focus shifted to functional protein synthesis, apoptosis, endocytosis, and lipid metabolism. This study offers valuable insights into the monitoring and management of combined environmental pollution.

Complexity of influences on atrazine phytoremediation of coexisting graphene oxide in water: Mitigating its phytotoxicity while decreasing plant removal contribution.

Phytoremediation is an efficient technology for the removal of herbicide atrazine (ATZ) contamination in water bodies, but its ability to reduce ATZ under combined pollution remains unclear, especially ATZ co-existing with the emerging pollutant graphene oxide (GO) that may have potential effects on ATZ fate, plants and microbes. Herein, we investigated the phytoremediation potential of an emergent plant (Iris pseudacorus) for ATZ and the response of bacteria in a hydroponic system with and without GO. The results showed that plants enhanced ATZ dissipation in water with the increased removal rate by a factor of 1.7-4.0. GO restricted ATZ uptake by plants, but favored ATZ bioconcentration in cell walls. The plant contributed most to changes in the bacterial communities, decreasing the alpha diversity, while enriching the functional categories involving in amino acid and carbohydrate metabolisms. These findings indicated that I. pseudacorus can be employed as an effective candidate of phytoremediation for ATZ co-existing with GO at environmentally relevant concentrations, tending to recruit bacteria with plant stress tolerance and growth-promotion activities more than with ATZ degradation activities; GO exerted a mitigating effect on ATZ stress improving the barrier function of cell walls, but decreased the contribution of plants to ATZ removal.

Algal EPS modifies the toxicity potential of the mixture of polystyrene nanoplastics (PSNPs) and triphenyl phosphate in freshwater microalgae Chlorella sp.

Triphenyl phosphate (TPP) and polystyrene nanoplastics (PSNPs) are prevalent freshwater contaminants obtained mainly from food packaging, textiles and electronics. Algal extracellular polymeric substances (EPS), a part of natural organic matter, may influence these pollutants' behaviour and toxicity. The presence of EPS can enhance the aggregation of TPP-PSNP mixtures, and reduce the bioavailability, and thus the toxicity potential. Understanding the mutual interactions between TPP, PSNPs, and EPS in the aquatic environment is a prerequisite for the environmental risk assessment of these chemicals. The study examines the toxicity effects of various surface-modified PSNPs (1 mg/L of plain, animated, and carboxylated) and TPP (0.05, 0.5, and 5 mg/L) in pristine and combined forms on freshwater microalgae, Chlorella sp., as a model organism. The physical-chemical interactions of algal EPS (10 mg/L) with PSNPs and TPP and their mixtures were studied. The toxicity potential of the PSNPs was estimated by quantifying growth inhibition, oxidative stress, antioxidant activity, and photosynthesis in the cells. TPP toxicity increased in the presence of the PSNPs, however the addition of algal EPS reduced the combined toxic effects. EPS plays a protective role by reducing oxidative stress and enhancing photosynthetic efficiency in the algal cells. The Pearson modeling analysis indicated a positive correlation between growth inhibition, and reactive oxygen species, malondialdehyde production. The cluster heatmap and correlation mapping revealed a strong correlation among the oxidative stress, growth inhibition, and photosynthetic parameters. The study clearly highlights the potential of EPS in mitigating the risk of mixed emerging pollutants in the aquatic environment.

Coupling polyethylene microplastics with other pollutants: Exploring their combined effects on plant health and technologies for mitigating toxicity.

The presence of microplastics in agricultural soils has raised concerns regarding their potential impacts on ecosystem health and plant growth. The introduction of microplastics into soil can alter its physicochemical properties, leading to adverse effects on plant development. Furthermore, the adsorption capabilities of microplastics may enhance the toxicity of soil pollutants, potentially resulting in detrimental effects on plant life. Large-sized microplastics may become adhered to root surfaces, impeding stomatal function and restricting nutrient uptake. Conversely, smaller microplastics and nano-plastics may be internalized by plants, causing cellular damage and genotoxicity. In addition, the presence of microplastics in soil can indirectly affect plant growth and development by altering the soil environment. Therefore, it is essential to investigate the potential impacts of microplastics on agricultural ecosystems and develop strategies to mitigate their effects. This review describes the adsorption power between polyethylene microplastics and pollutants (heavy metals, polycyclic aromatic hydrocarbons and antibiotics) commonly found in agricultural fields and the factors affecting the adsorption process. Additionally, the direct and indirect effects of microplastics on plants are summarized. Most of the single or combined microplastic contaminants showed negative effects on plant growth, with a few beneficial effects related to the characteristics of the microplastics and environmental factors. Currently microbial action and the application of soil conditioners or plant growth promoters can alleviate the effects of microplastics on plants to a certain extent. In light of the complex nature of soil environments, future research should concentrate on mitigate and control these interactions and the impact of compound pollution on ecosystems.

Efficient and simultaneous immobilization of fluoride and lead in water and tea garden soil by bayberry tannin foam loaded zirconium.

Nowadays, human activities intensified the combined pollution of fluoride and lead in acidic tea garden soil. The key to eliminating this combined pollution is to immobilize pollutants simultaneously, thus preventing their migration from tea garden soil to tea trees. In this paper, the natural product bayberry tannin was employed as raw material to fabricate functional materials (TF-Zr) for simultaneous adsorption of fluorine (F) and lead (Pb) in water and soil by the reactivity of tannin with Pb2+ and the affinity of Zr with F. SEM-Mapping, EDS, FT-IR, XPS were utilized to probe the immobilization mechanisms. The results showed that TF-Zr could simultaneously and efficiently adsorb F- and Pb2+ from water with the adsorption capacity of 5.02 mg/g (Pb) and 4.55 mg/g (F). The adsorption processes were both in accordance with the proposed secondary kinetic adsorption model. Besides, the presence of F- promoted the adsorption of Pb2+ by TF-Zr. The materials were applied into tea garden soil to explore its effect on the variation of F and Pb forms in the soil. It was found that the proportion of water-soluble fluorine, exchangeable fluorine and exchangeable lead in the tea garden soil decreased significantly, while the proportion of residual fluorine and lead increased evidently, illustrating TF-Zr possessed eximious fixation effect on the highly reactive fluorine and lead in the soil and facilitated their conversion to the more stable residue state. Therefore, TF-Zr can be used for the efficient and simultaneous immobilization of fluorine and lead in water and tea garden soil.

Unveiling the impacts of microplastic pollution on soil health: A comprehensive review.

Soil contamination by microplastics (MPs) has emerged as a significant global concern. Although traditionally associated with crop production, contemporary understanding of soil health has expanded to include a broader range of factors, including animal safety, microbial diversity, ecological functions, and human health protection. This paradigm shifts underscores the imperative need for a comprehensive assessment of the effects of MPs on soil health. Through an investigation of various soil health indicators, this review endeavors to fill existing knowledge gaps, drawing insights from recent studies conducted between 2021 and 2024, to elucidate how MPs may disrupt soil ecosystems and compromise their crucial functions. This review provides a thorough analysis of the processes leading to MP contamination in soil environments and highlights film residues as major contributors to agricultural soils. MPs entering the soil detrimentally affect crop productivity by hindering growth and other physiological processes. Moreover, MPs hinder the survival, growth, and reproductive rates of the soil fauna, posing potential health risks. Additionally, a systematic evaluation of the impact of MPs on soil microbes and nutrient cycling highlights the diverse repercussions of MP contamination. Moreover, within soil-plant systems, MPs interact with other pollutants, resulting in combined pollution. For example, MPs contain oxygen-containing functional groups on their surfaces that form high-affinity hydrogen bonds with other pollutants, leading to prolonged persistence in the soil environment thereby increasing the risk to soil health. In conclusion, we succinctly summarize the current research challenges related to the mediating effects of MPs on soil health and suggest promising directions for future studies. Addressing these challenges and adopting interdisciplinary approaches will advance our understanding of the intricate interplay between MPs and soil ecosystems, thereby providing evidence-based strategies for mitigating their adverse effects.

Improved microbial-plant soil bioremediation of PAHs and heavy metal through in silico methods.

The combined pollution of polycyclic aromatic hydrocarbons (PAHs) and organic cadmium (Cd) in farmland soils, and the field controlling strategy need to be studied urgently. In this study, 5 PAHs, 5 Cd and 11 soil conditioners were selected to explore the co-exposure risk and remediation efficiency. Firstly, a significant combination Fl-alkylalkoxy cadmium was obtained using forward and reverse methods coupling variation coefficient methods (the combined pollution value was 0.173). Secondly, the interaction energy of microbial degradation / plant absorption of Fl under Cd stress, and microbial mineralization / plant absorption of alkylalkoxy cadmium under PAHs stress were characterized using factorial experimental design, molecular docking and molecular dynamics simulation. The combined pollution of alkylalkoxy cadmium and dialkyl cadmium, phenanthrene and Benzo [a] pyrene was significant (synergistic contribution rates were 17.58 % and 19.22 %, respectively). In addition, 6 soil conditioners with significant efficiency were selected to design Taguchi orthogonal experimental schemes, indicating the microbial degradation / mineralization and plant absorption were significantly effective (the maximum increase of remediation efficiency was 93.81 %) under the combinations (i.e., trratone, coumarol, fulvamic acid, potassium fertilizer and others, etc.). Finally, it was found that the soil conditioners affected the hydrophobic groups and forces, and the efficiency was proportional to the highest peak value and minimum distance in the RDF curve. This study identifies the risk characteristics of co-exposure of PAHs and Cd and screens effective soil conditioners, providing theoretical guidance for risk controlling.

Performances of a novel BAF with ferromanganese oxide modified biochar (FMBC) as the carriers for treating antibiotics, nitrogen and phosphorus in aquaculture wastewater.

In this paper, a biological aerated filter (BAF) based on ferromanganese oxide-biochar (FMBC) was constructed to investigated the removal performance and mechanism for conventional pollutants and four kinds of antibiotic, in contrast of conventional zeolite loaded BAF (BAF-A) and bamboo biochar filled BAF (BAF-B). Results showed that the average removal efficiency of total nitrogen (TN), total phosphorus (TP) and antibiotics in a FMBC-BAF (named by BAF-C) were 52.97 ± 2.27%, 51.58 ± 1.92% and 70.36 ± 1.00% ~ 81.65 ± 0.99% respectively in running period (39-100 d), which were significantly higher than those of BAF-A and BAF-B. In the BAF-C, the expression of denitrification enzyme activities and the secretion of extracellular polymeric substance (EPS) especially polyprotein (PN) were effectively stimulated, as well as accelerated electron transfer activity (ETSA) and lower electrochemical impedance spectroscopy (EIS) were acquired. After 100 days of operation, the abundance of nitrogen, phosphorus and antibiotic removal functional bacteria like Sphingorhabdus (4.52%), Bradyrhizobium (1.98%), Hyphomicrobium (2.49%), Ferruginibacter (7.80%), unclassified_f_Blastoca tellaceae (1.84%), norank_f_JG30-KF-CM45 (6.82%), norank_f_norank_o_SBR1031 (2.43%), Nitrospira (2.58%) norank_f_Caldilineaceae (1.53%) and Micropruina (1.11%) were enriched. Mechanism hypothesis of enhanced performances of nutrients and antibiotics removal pointed that: The phosphorus was removed by adsorption and precipitation, antibiotics removal was mainly achieved through the combined action of adsorption and biodegradation, while nitrogen removal was realized by biologic nitrification and denitrification in a FMBC-BAF for aquaculture wastewater treatment.

Degradation of Three Herbicides and Effect on Bacterial Communities under Combined Pollution.

Pesticide residues in soil, especially multiple herbicide residues, cause a series of adverse effects on soil properties and microorganisms. In this work, the degradation of three herbicides and the effect on bacterial communities under combined pollution was investigated. The experimental results showed that the half-lives of acetochlor and prometryn significantly altered under combined exposure (5.02-11.17 d) as compared with those of individual exposure (4.70-6.87 d) in soil, suggesting that there was an antagonistic effect between the degradation of acetochlor and prometryn in soil. No remarkable variation in the degradation rate of atrazine with half-lives of 6.21-6.85 d was observed in different treatments, indicating that the degradation of atrazine was stable. 16S rRNA high-throughput sequencing results showed that the antagonistic effect of acetochlor and prometryn on the degradation rate under combined pollution was related to variation of the Sphingomonas and Nocardioide. Furthermore, the potential metabolic pathways of the three herbicides in soil were proposed and a new metabolite of acetochlor was preliminarily identified. The results of this work provide a guideline for the risk evaluation of combined pollution of the three herbicides with respect to their ecological effects in soil.

[Screening and Stress Responsive Characteristics of Potential Hyperaccumulator of Pb, Zn, and Cd Compound Heavy Metals].

To screen for Pb, Zn, and Cd composite heavy metal hyperaccumulator plants, a survey, sampling, and analysis of dominant plants in typical lead-zinc mines and smelter areas in Baoji City were conducted. Potential Pb, Zn, and Cd composite heavy metal hyperaccumulator plants were initially screened, and a pot experiment of soil cultivation was carried out to observe the response characteristics of chlorophyll （chlorophyll a, chlorophyll b, carotenoids, and total chlorophyll）, antioxidant enzymes （SOD, CAT, and POD）, and other physiological indicators （MDA and proline） under the stress of Pb, Zn, and Cd composite heavy metals. A field experiment was also conducted to further verify and determine their enrichment ability for Pb, Zn, and Cd composite heavy metals, aiming to provide scientific basis and technical support for the remediation of Pb, Zn, and Cd composite heavy metal-polluted soil. The field survey revealed that Symphytum officinale L. met the international hyperaccumulator plant index requirements for the enrichment of Pb, Zn, and Cd, with enrichment quantity, bioconcentration factor （BCF）, and transfer factor （TF） all meeting the requirements. It was a potential hyperaccumulator plant for Pb, Zn, and Cd composite heavy metals. The soil cultivation pot experiment showed that as the gradient of Pb, Zn, and Cd composite heavy metal stress increased, the content of chlorophyll a, chlorophyll b, and total chlorophyll in S. officinale L. leaves gradually decreased, causing disruption to the plant's photosynthetic system when the gradient was greater than or equal to IV. The chlorophyll content in Ricinus communis L. leaves exhibited a "low-stimulation-high-inhibition" phenomenon, while excessive stress stimulated the activation of its own protective systems, leading to reduced toxicity. In addition, there were significant differences （P < 0.05） in the content of chlorophyll a, chlorophyll b, carotenoids, and total chlorophyll between S. officinale L. and R. communis L. both compared to the control treatment and between stress gradients. The SOD activity in the leaves of S. officinale L. and R. communis L. showed a trend of increasing first, then decreasing, and then increasing. The CAT activity in the leaves of S. officinale L. exhibited a "low-stimulation-high-inhibition" effect, whereas the CAT activity in the leaves of R. communis L. showed a trend of continuous decrease. The POD activity in the leaves of S. officinale L. generally increased, whereas in the leaves of R. communis L., it increased first, then decreased, and then increased. The MDA content in the leaves of S. officinale L. generally decreased, whereas in the leaves of R. communis L., it exhibited an upward trend. In addition, whether compared to the control between stress gradients, there were significant differences （P < 0.05） in the SOD, CAT, POD, MDA, and proline content of S. officinale L. and R. communis L. The field experiment results indicated that S. officinale L. could meet the hyperaccumulator plant index requirements for the enrichment of Pb, Zn, and Cd, making it a potential germplasm resource for Pb, Zn, and Cd composite heavy metal hyperaccumulator plants. It can be an ideal choice for the remediation of Pb, Zn, and Cd composite heavy metal-polluted soil.

Adsorption behavior and mechanism of heavy metals onto microplastics: A meta-analysis assisted by machine learning.

Microplastics (MPs) have the potential to adsorb heavy metals (HMs), resulting in a combined pollution threat in aquatic and terrestrial environments. However, due to the complexity of MP/HM properties and experimental conditions, research on the adsorption of HMs onto MPs often yields inconsistent findings. To address this issue, we conducted a comprehensive meta-analysis assisted with machine learning by analyzing a dataset comprising 3340 records from 134 references. The results indicated that polyamide (PA) (ES = -1.26) exhibited the highest adsorption capacity for commonly studied HMs (such as Pb, Cd, Cu, and Cr), which can be primarily attributed to the presence of C=O and N-H groups. In contrast, polyvinyl chloride (PVC) demonstrated a lower adsorption capacity, but the strongest adsorption strength resulting from the halogen atom on its surface. In terms of HMs, metal cations were more readily adsorbed by MPs compared with metalloids and metal oxyanions, with Pb (ES = -0.78) exhibiting the most significant adsorption. As the pH and temperature increased, the adsorption of HMs initially increased and subsequently decreased. Using a random forest model, we accurately predicted the adsorption capacity of MPs based on MP/HM properties and experimental conditions. The main factors affecting HM adsorption onto MPs were HM and MP concentrations, specific surface area of MP, and pH. Additionally, surface complexation and electrostatic interaction were the predominant mechanisms in the adsorption of Pb and Cd, with surface functional groups being the primary factors affecting the mechanism of MPs. These findings provide a quantitative summary of the interactions between MPs and HMs, contributing to our understanding of the environmental behavior and ecological risks associated with their correlation.

Response of wheat (Triticum aestivum L. cv.) to the coexistence of micro-/nanoplastics and phthalate esters alters its growth environment.

Micro- and nano-plastics (MPs/NPs) have emerged as a global pollutant, yet their impact on the root environment of plants remains scarcely explored. Given the widespread pollution of phthalate esters (PAEs) in the environment due to the application of plastic products, the co-occurrence of MPs/NPs and PAEs could potentially threaten the growth medium of plants. This study examined the combined effects of polystyrene (PS) MPs/NPs and PAEs, specifically dibutyl phthalate and di-(2-ethylhexyl) phthalate, on the chemical properties and microbial communities in a wheat growth medium. It was observed that the co-pollution with MPs/NPs and PAEs significantly increased the levels of oxalic acid, formic acid, and total organic carbon (TOC), enhanced microbial activity, and promoted the indigenous input and humification of dissolved organic matter, while slightly reducing the pH of the medium solution. Although changes in chemical indices were primarily attributed to the addition of PAEs, no interaction between PS MPs/NPs and PAEs was detected. High-throughput sequencing revealed no significant change in microbial diversity within the media containing both PS MPs/NPs and PAEs compared to the media with PS MPs/NPs alone. However, alterations in energy and carbohydrate metabolism were noted. Proteobacteria dominated the bacterial communities in the medium solution across all treatment groups, followed by Bacteroidetes and Verrucomicrobia. The composition and structure of these microbial communities varied with the particle size of the PS in both single and combined treatments. Moreover, variations in TOC, oxalic acid, and formic acid significantly influenced the bacterial community composition in the medium, suggesting they could modulate the abundance of dominant bacteria to counteract the stress from exogenous pollutants. This research provides new insights into the combined effects of different sizes of PS particles and another abiotic stressor in the wheat root environment, providing a critical foundation for understanding plant adaptation in complex environmental conditions.

Plant Cadmium Toxicity and Biomarkers Are Differentially Modulated by Degradable and Nondegradable Microplastics in Soil.

The impact of microplastics (MPs) as emerging pollutants on plant heavy metal toxicity has been extensively reported in vegetable-soil systems over recent years. However, little attention has been given to cultivar variations between degradable and non-degradable MPs. This study investigated the effects of degradable polylactic acid (PLA) and nondegradable polypropylene (PP) MPs on plant growth and biomarker (malonaldehyde (MDA) and antioxidant enzymes) performance in Cd-contaminated arable soil. The results show that both types of MPs significantly impacted plant biomass and biomarker contents across all three Cd levels. The degree of impact was significantly sensitive to both the type and dose of MPs, as they reduced the soil pH and cation exchange capacity (CEC) while increasing soil dissolved organic carbon (DOC), microbial biomass carbon, and nitrogen. PP exhibited greater root growth inhibition and phytotoxicity at higher doses of 1% and 5% compared to PLA. Specifically, the highest MDA contents were 1.44 and 2.20 mmol mg-1 protein for shoots and roots, respectively, in the 5% PLA treatment under a 10.1 mg kg-1 Cd level, which were 1.22 and 1.18 times higher than those in corresponding treatments of 5% PP. Overall, PLA had less significant effects on plant phytotoxicity, Cd availability, and soil properties compared to PP. Regression pathway analysis indicated that MPs increased shoot Cd uptake by altering both soil physical-chemical and microbial characteristics. Among the soil variables, pH, CEC, and Cd bioavailability were found to play vital roles. Yet, no single variable acts alone in the mechanism for plant Cd uptake. PLAs are suggested to replace conventional non-biodegradable plastics to control environmental MP pollution, particularly in agricultural systems with higher Cd contamination. However, the long-term effects of the by-products generated during the biodegradation process require further investigation.

Enhanced Cadmium Adsorption Dynamics in Water and Soil by Polystyrene Microplastics and Biochar.

Microplastics (MPs) are prevalent emerging pollutants in soil environments, acting as carriers for other contaminants and facilitating combined pollution along with toxic metals like cadmium (Cd). This interaction increases toxic effects and poses substantial threats to ecosystems and human health. The objective of this study was to investigate the hydrodynamic adsorption of Cd by conducting experiments where polystyrene microplastics (PS) and biochar (BC) coexisted across various particle sizes (10 µm, 20 µm, and 30 µm). Then, soil incubation experiments were set up under conditions of combined pollution, involving various concentrations (0.5 g·kg-1, 5 g·kg-1, 50 g·kg-1) and particle sizes of PS and BC to assess their synergistic effects on the soil environment. The results suggest that the pseudo-second-order kinetic model (R2 = 0.8642) provides a better description of the adsorption dynamics of Cd by PS and BC compared to the pseudo-first-order kinetic model (R2 = 0.7711), with an adsorption saturation time of 400 min. The Cd adsorption process in the presence of PS and BC is more accurately modeled using the Freundlich isotherm (R2 > 0.98), indicating the predominance of multilayer physical adsorption. The coexistence of 10 µm and 20 µm PS particles with BC enhanced Cd absorption, while 30 µm PS particles had an inhibitory effect. In soil incubation experiments, variations in PS particle size increased the exchangeable Cd speciation by 99.52% and decreased the residual speciation by 18.59%. The addition of microplastics notably impacted the exchangeable Cd speciation (p < 0.05), with smaller PS particles leading to more significant increases in the exchangeable content-showing respective increments of 45.90%, 106.96%, and 145.69%. This study contributes to a deeper understanding of the mitigation mechanisms of biochar in the face of combined pollution from microplastics and heavy metals, offering theoretical support and valuable insights for managing such contamination scenarios.

Multi-interface interaction mechanism of pulp reject-based flocculants for the removal of antibiotics and its combined pollutants.

The efficient removal of antibiotics and its combined pollutants is essential for aquatic environment and human health. In this study, a lignin-based organic flocculant named PRL-VAc-DMC was synthesized using pulp reject as the raw material, with vinyl acetate (VAc) and methacryloxyethyltrimethyl ammonium chloride (DMC) as the grafting monomers. A series of modern characterization methods were used to confirm the successful preparation of PRL-VAc-DMC and elucidate its polymerization mechanism. It was found that the Ph-OH group and its contiguous carbon atoms of lignin served as the primary active sites to react with grafting monomers. Flocculation experiments revealed that PRL-VAc-DMC could react with tetracycline (TC) through π-π* interaction, hydrophobic interaction, hydrogen bonding, and electrostatic attraction. With the coexistence of humic acid (HA) and Kaolin, the aromatic ring, hydroxyl, and amide group of TC could react with the benzene ring, hydroxyl group, and carboxyl group of HA, forming TC@HA@Kaolin complexes with Kaolin particles acting as the hydrophilic shell. The increase in particle size, electronegativity, and hydrophily of TC@HA@Kaolin complexes facilitated their interaction with PRL-VAc-DMC through strong interfacial interactions. Consequently, the presence of HA and Kaolin promoted the removal of TC. The synergistic removal mechanism of TC, HA, and Kaolin by PRL-VAc-DMC was systematically analyzed from the perspective of muti-interface interactions. This paper is of great significance for the comprehensive utilization of pulp reject and provides new insights into the flocculation mechanism at the molecular scale.

Insights into the combined toxicity and mechanisms of BDE-47 and PFOA in marine blue mussel: An integrated study at the physiochemical and molecular levels.

The coexistence of multiple emerging contaminants imposes a substantial burden on the ecophysiological functions in organisms. The combined toxicity and underlying mechanism requires in-depth understanding. Here, marine blue mussel (Mytilus galloprovincialis L.) was selected and exposed to 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and perfluorooctanoic acid (PFOA) individually and in combination at environmental related concentrations to elucidate differences in stress responses and potential toxicological mechanisms. Characterization and comparison of accumulation, biomarkers, histopathology, transcriptomics and metabolomics were performed. Co-exposure resulted in differential accumulation patterns, exacerbated histopathological alterations, and different responses in oxidative stress and biomarkers for xenobiotic transportation. Moreover, the identified differentially expressed genes (DEGs) and differential metabolites (DEMs) in mussels were found to be annotated to different metabolic pathways. Correlation analyses further indicated that DEGs and DEMs were significantly correlated with the above biomarkers. BDE-47 and PFOA altered the genes and metabolites related to amino acid metabolism, energy and purine metabolism, ABC transporters, and glutathione metabolism to varying degrees, subsequently inducing accumulation differences and combined toxicity. Furthermore, the present work highlighted the pivotal role of Nrf2-keap1 detoxification pathway in the acclimation of M. galloprovincialis to reactive oxygen species (ROS) stress induced by BDE-47 and PFOA. This study enabled more comprehensive understanding of combined toxic mechanism of multi emerging contaminants pollution.

Meta-analysis reveals the combined effects of microplastics and heavy metal on plants.

The combined pollution of microplastics and heavy metals is becoming increasingly serious, and its effects on toxicology and heavy metal accumulation of plants are closely related to crop yield and population health. Here, we collected 57 studies to investigate the effect of microplastics on heavy metal accumulation in plants and their combined toxicity. An assessment was conducted to discover the primary pollutant responsible for the toxicity of combined pollution on plants. The study examined the influence of microplastic characteristics, heavy metal characteristics, and experimental methods on this pollutant. The results showed that combined toxicity of plants was more similar to heavy metals, whereas microplastics interacted with heavy metals mainly by inducing oxidative stress damage. Culture environment, heavy metal type, experimental duration, microplastic concentration and microplastic size were the main factors affecting heavy metal accumulation in plants. There was a negative correlation between experimental duration, microplastic concentration and microplastic size with heavy metal accumulation in plants. The interactions among influencing factors were found, and microplastic biodegradation was the core factor of the strong interaction. These results provided comprehensive insights and guiding strategies for environmental and public health risks caused by the combined pollution of microplastics and heavy metals.

Effects of microplastics and cadmium co-contamination on soil properties, maize (Zea mays L.) growth characteristics, and cadmium accumulation in maize in loessial soil-maize systems.

Microplastics (MPs) are pervasive pollutants found in agricultural soils, yet research on the combined impacts of MPs and heavy metals on soil-plant systems remains limited. This study investigates the combined impact of low-density polyethylene (LDPE) microplastics (L: 1 mm, S: 100 µm, 0.1%, 1%) and Cd on soil properties, available Cd content, maize growth, and Cd accumulation by mazie through pot experiments. The findings unveiled notable impacts of the treatment groups, namely MP-L0.1%, MP-S0.1%, MP-L1%, and MP-S1%, on soil organic carbon (SOC), maize height, and catalase (CAT) activity (P < 0.05). The dosage of MPs significantly influenced maize height, MP-S0.1% treatment resulted in a 5.6% reduction, while the other groups had insignificant effects. Particle size and dosage significantly affected SOC and CAT (P < 0.01). The MP-L1% and MP-S1% groups resulted in increases of SOC by 121.5% and 281.0%, respectively. CAT reductions were 32.6%, 62.8%, 41.9%, and 34.9% in MP-L0.1%, MP-S0.1%, MP-L1%, and MP-S1% groups, individually. The Cd treatment induced a significant decrease in soil cation exchange capacity (CEC), maize stem diameter, and root length, accompanied by significant increases in maize plant height, malondialdehyde (MDA), CAT, and superoxide dismutase (SOD) activities. Combined LDPE and Cd contamination had significant effects on maize height and Cd content in leaves. Specifically, MP-L0.1%+Cd, MP-S0.1%+Cd, MP-L1%+Cd, and MP-S1%+Cd reduced maize height by 4.1%, 4.5%, 8.7%, and 13.8%, respectively. The co-presence of LDPE and Cd increased available Cd content in soil while elevating Cd concentration in maize shoots and roots, with a notable 25.5% increase in Cd concentration in maize leaves in the MP-L1%+Cd group compared to the Cd group. Furthermore, LDPE effects on soil-plant systems varied depending on particle size and dosage. This research provides important perspectives on evaluating the concurrent contamination and potential dangers of MPs and toxic metals in soil-plant environments.

Microplastics increase cadmium absorption and impair nutrient uptake and growth in red amaranth (Amaranthus tricolor L.) in the presence of cadmium and biochar.

Microplastic (MP) pollution in terrestrial ecosystems is gaining attention, but there is limited research on its effects on leafy vegetables when combined with heavy metals. This study examines the impact of three MP types-polyethylene (PE), polyethylene terephthalate (PET), and polystyrene (PS)-at concentrations of 0.02, 0.05, and 0.1% w/w, along with cadmium (Cd) and biochar (B), on germination, growth, nutrient absorption, and heavy metal uptake in red amaranth (Amaranthus tricolor L.). We found that different MP types and concentrations did not negatively affect germination parameters like germination rate, relative germination rate, germination vigor, relative germination vigor, and germination speed. However, they increased phytotoxicity and decreased stress tolerance compared to an untreated control (CK1). The presence of MPs, particularly the PS type, reduced phosphorus and potassium uptake while enhancing Cd uptake. For example, treatments PS0.02CdB, PS0.05CdB, and PS0.1CdB increased Cd content in A. tricolor seedlings by 158%, 126%, and 44%, respectively, compared to the treatment CdB (CK2). Additionally, MP contamination led to reduced plant height, leaf dry matter content, and fresh and dry weights, indicating adverse effects on plant growth. Moreover, the presence of MPs increased bioconcentration factors and translocation factors for Cd, suggesting that MPs might act as carriers for heavy metal absorption in plants. On the positive side, the addition of biochar improved several root parameters, including root length, volume, surface area, and the number of root tips in the presence of MPs, indicating potential benefits for plant growth. Our study shows that the combination of MPs and Cd reduces plant growth and increases the risk of heavy metal contamination in food crops. Further research is needed to understand how different MP types and concentrations affect various plant species, which will aid in developing targeted mitigation strategies and in exploring the mechanisms through which MPs impact plant growth and heavy metal uptake. Finally, investigating the potential of biochar application in conjunction with other amendments in mitigating these effects could be key to addressing MP and heavy metal contamination in agricultural systems.