Serum microRNA profiling for the identification of predictive molecular markers of the response to controlled ovarian stimulation.

OBJECTIVE: To identify potential microRNA (miRNA) biomarkers of poor, normal and hyperresponse to controlled ovarian stimulation (COS). METHODS: In the present study, we assessed 40 serum samples from patients undergoing COS. We used ten samples to standardize miRNAs detection in the serum. The remaining 30 samples were split into three groups depending on the patient's response to COS: poor response (PR group, n=10), normal response (NR group, n=10), and hyperresponse (HR group, n=10). Aberrantly expressed miRNAs were identified using a large-scale expression analysis platform. Gene set enrichment analysis was performed to assess the biological processes potentially modulated by the identified miRNAs. RESULTS: Twenty-two miRNAs were detected only in the PR or HR groups when compared with the NR group. From those, 11 presented poor dissociation curves and were excluded from further analysis. A bioinformatics analysis revealed that the selected 11 miRNAs target several genes involved in GnRH, estrogen and prolactin signaling, oocyte maturation, female pregnancy, and meiosis. CONCLUSION: The large-scale analysis of miRNA expression identified distinct miRNA profiles for poor and hyperresponse to COS, which potentially modulate key processes for human assisted reproduction. All evidence suggests that the serum microRNA profiling may discriminate patients who will respond in an exacerbated manner from those who will respond insufficiently to COS. Further studies may validate these miRNAs, enabling the individualization of treatment and more successful outcomes.

Definitions of Pediatric Functional Abdominal Pain Disorders and Outcome Measures: A Systematic Review.

OBJECTIVE: To systematically review definitions of functional abdominal pain orders (FAPDs) and outcome measures used in therapeutic randomized controlled trials in pediatric FAPDs adhering to the Outcome Measures in Rheumatology recommendations. STUDY DESIGN: Cochrane, MEDLINE, Embase, and Cinahl databases were systematically searched from inception to April 2018. English-written therapeutic randomized controlled trials concerning FAPDs in children aged 4-18 years were included. Definitions of FAPDs, interventions, outcome measures, measurement instruments, and outcome assessors of each study were tabulated descriptively. Quality was assessed using the Delphi List. RESULTS: A total of 4771 articles were found, of which 64 articles were included (n = 25, 39% of high methodologic quality). The Rome III (50%), Rome II (17%), Apley (16%), and author-defined (17%) criteria were used to define FAPDs. Fourteen studies (22%) assessed a pharmacologic, 25 (39%) a dietary, and 25 (39%) a psychosocial intervention. Forty-four studies (69%) predefined their primary outcomes. In total, 211 reported predefined outcome measures were grouped into 23 different outcome domains; the majority being patient-reported (n = 27, 61%). Of the 14 studies that evaluated a pharmacologic intervention, 12 (86%) reported on adverse events. CONCLUSIONS: Studies on pediatric FAPDs are of limited methodologic quality and show large heterogeneity and inconsistency in defining FAPDs and outcome measures used. Development of a core outcome set is needed to make comparison between intervention studies possible.

Ovarian response to stimulation and suboptimal endometrial development are associated with adverse perinatal outcomes in intracytoplasmic sperm injection cycles.

OBJECTIVE: To study which factors affect perinatal outcomes in intracytoplasmic sperm injection (ICSI) cycles. METHODS: Data was obtained from 402 live births born to 307 patients undergoing ICSI cycles in a private university-affiliated IVF center between Jan/2014 and Dec/2015. The influences of the cycles' characteristics on the number of gestational weeks to livebirth (GW), baby birth weight (BW), and baby birth length (BL) were evaluated by linear regression models, adjusted for maternal age and body mass index, number of transferred embryos, number of gestational sacs, and number of born infants. In a subsequent analysis, GW, BW and baby sex were utilized for cycle classification into the groups Appropriate for gestational age (AGA n=256) and Small for gestational age (SGA n=146), which were compared by general linear models adjusted for the same confounder variables. RESULTS: The number of follicles (ß=-0.069 p=0.018) and retrieved oocytes (ß=-0.087 p=0.049) were negatively correlated with BL. The endometrial thickness was positively correlated with GW (ß=0.198 p=0.003) and BW (ß=28.351 p=0.044). When each baby was classified into AGA and SGA groups, it was observed that SGA babies were derived from cycles with higher estradiol levels at hCG day (SGA: 3897.01±550.35 vs. AGA: 2324.78±101.86 p=0.006) and higher number of retrieved oocytes (SGA: 16.70±1.78 vs. AGA: 12.92±0.42 p=0.042). The endometrial thickness was significantly lower in the SGA group (SGA: 10.2±0.23 vs. AGA: 11.68±0.17 vs. p=0.029). CONCLUSION: Higher ovarian response to stimulation and suboptimal endometrial development are associated with adverse perinatal outcomes in ICSI cycles.

Implicancia pronóstica de características basales en trombocitopenia esencial / Prognostic implication of basal characteristics in essential thrombocythemia

OBJETIVO: evaluar asociación entre características basales de Trombocitemia Esencial (TE) y eventos. Materiales-métodos: estudio observacional retrospectivo, datos capturados prospectivamente. Población: adultos con TE confirmada seguidos en CH del IIHEMA. Resultados: 67 pacientes evaluables. Md 68 años. Femenino 44, Masculino 23. Seguimiento Md 6 años. Score riesgo Alto 40, Intermedio 15, Bajo 12. Hematocrito Md 41%. Hemoglobina Md 13.4 g/dL. Leucocitos Md 9.7x109/L. Plaquetas Md 852x109/L. Esplenomegalia 12. Cariotipo normal 40, alterado 5. JAK2 V617F positivo 29/41. BCR/ABL1 negativo 45/45. FRV 40. Eventos: 67. Sangrado, 21 pacientes, presentó asociación con leucocitosis: Md 12.2x109/L en el grupo con sangrado y 8.8x109/L en el grupo sin evento (p=0.003). El 76% del grupo con sangrado y el 36,96% del grupo sin evento tenían leucocitos >10.0x109/L (p= 0.003). Trombocitosis Md 1.204x109/L en el grupo con sangrados y 814.5x109/L en el grupo sin evento (p=0.0098), no alcanzó significación estadística al comparar proporción de pacientes con recuento normal (p=0.46). Tromboembolia, 16 pacientes, se asoció con leucocitosis (Md 11.9x109/L en el grupo con tromboembolia y 9.2x109/L en el grupo sin evento (p=0.02). 75% del grupo con eventos y 41% del grupo sin eventos tenían leucocitos >10.0x109/L (p=0.018)) y con trombocitosis (Md 1.182x109/L en el grupo con tromboembolia y 809x109/L en el grupo sin evento (p=0.04), pero no alcanzó significación estadística al comparar proporción de pacientes con recuento normal (p=0.5)). CONCLUSIÓN: la asociación entre trombocitosis extrema/leucocitosis y sangrado coincidió con la literatura; leucocitosis se asoció a tromboembolia. El JAK2 V617F no mostró asociación: analizaremos prospectivamente aumentando la población para esclarecer asociación y posible rol de terapias dirigidas en esta entidad. (AU)

OBJETIVE: asses association basal characteristics of Essential Trombocitemia (ET) and events. MATERIALS-METHODS: retrospective observational study, data captured prospectively. Population: adults with confirmed ET treated in CH of IHEMA. RESULTS: 67 evaluable patients. Md 68-years-old. Female 44, Male 23. Follow up Md 6 years. Risk Score High 40, Intermeddle 15, Low 12. Hematocrit Md 41%. Hemoglobin Md 13.4 g/dL. Leukocytes Md 9.7x109/L. Platelets Md 852x109/L. Splenomegaly 12. Normal karyotype 40, abnormal 5. JAK2V617F positive 29/41. BCR-ABL1 negative 45/45. Vascular risk factors 40. EVENTS: 67. Bleeding, 21 patients, showed association with leukocytosis: Md 12.2x109/L in the group with bleeding and 8.8x109/L in the group without event (p=0.003). Leukocytes >10.0x109/L was seen in 76% of the group with bleeding versus 36.96% of the group without event (p= 0.003). Thrombocytosis Md 1.204x109/L in the group with bleeding and 814.5x109/L in the group without event (p=0.0098), but did not reach statistical significance when comparing with the proportion of patients with normal counts (p=0.46). Thromboembolism, 16 patients, showed association with both leukocytosis (Md 11.9x109/L in the group with thromboembolism and 9.2x109/L in the group without event (p=0.02). Leukocytosis >10.0x109/L was seen in 75% of the group with events and 41% of the group without event (p=0.018)) and with thrombocytosis (Md 1.182x109/L in the group with thromboembolism and 809x109/L in the group without event (p=0.04), but did not reach statistical significance when comparing with the proportion of patients with normal counts (p=0.5)). CONCLUSION: the association between extreme thrombocytosis/leukocytosis and bleeding was consistent with literature; leukocytosis was associated also with thromboembolism. JAK2 V617F mutated did not show association, we will prospectively analyze increasing the population to clarify its association and possible role of target therapies in this disease. (AU)

Application of a single-chain fragment variable (scFv) antibody for the confirmatory diagnosis of hydatid disease in non-endemic areas

Background: Hydatid disease is a serious parasitic disease threatening public health. Because of its rarity in non-endemic coastal areas, determining the nature and origin of a chronic, enlarged liver cystic mass is challenging in these regions. Under these circumstances, physicians need a confirmatory diagnostic tool beyond immunological and radiological examinations. This study investigated a novel human single-chain fragment variable (scFv) antibody for the confirmative diagnosis of 18 atypical hydatid disease cases in non-endemic coastal areas. Results: A scFv antibody against cystic echinococcosis was produced by genetic engineering and then applied to the immunohistochemical diagnosis of 18 cases of cystic echinococcosis presented in non-endemic coastal areas. The diagnosis of these cases by ultrasound and serum-based examinations was inconclusive. The 750 bp scFv antibody gene was expressed in COS-7 cells, and the antibody localized in the cytoplasm. The scFv antibody can detect the germinal layer and protoscolices of actively growing cysts but not of the degenerating protoscolices and has a diagnostic efficiency higher than that of single serum or ultrasound testing (P b 0.05). The combined use of scFv antibodies with serology and ultrasound diagnostics results in a diagnostic efficiency comparable to that of surgery. The scFv antibody can be used as a confirmatory test for the diagnosis of hydatid disease in non-endemic areas, providing a beneficial supplementary diagnostic method that complements traditional immune testing and ultrasonic radiology and thus helping physicians to effectively differentiate hydatid disease.

The predictive value of serum concentrations of anti-Müllerian hormone for oocyte quality, fertilization, and implantation.

OBJECTIVE: This study aimed to identify a possible correlation between serum levels of anti-Müllerian hormone (AMH) and oocyte quality, embryo developmental competence, and implantation potential. METHODS: 4488 oocytes obtained from 408 patients undergoing ICSI cycles were evaluated. Oocyte dimorphisms, embryo quality on days two and three, blastocyst formation competence, fertilization rates, implantation rates, and pregnancy rates were correlated with serum levels of AMH using Pearson's correlation coefficient and regression analysis. RESULTS: A positive correlation was observed between serum levels of AMH and number of retrieved oocytes (CC: 0.600, p<0.001), fertilization rate (CC:0.595, p=0.048), and number of obtained embryos (CC:0.495, p<0.001). AMH did not affect the quality of cleavage stage embryos or the chance of blastocyst formation. However, AMH levels affected oocyte quality (OR:0.75, CI 0.44-0.96, p<0.001), and implantation (CC:0,116, p=0.031) and pregnancy (OR:1.22, CI:1.03-1.53, p<0.001) rates. CONCLUSION: Serum levels of AMH are a useful predictor of ovarian response to COS, oocyte quality, and fertilization. However, AMH levels may also compromise clinical outcomes; lower AMH levels did not impair embryo development.

Vibrational spectroscopy and conformation of S-ethyl thioacetate: CH3COSCH2CH3 and comparison with -C(O)S- and -C(O)O- compounds.

The molecular structure and conformational properties of S-ethyl thioacetate, CH3COSCH2CH3, were determined in the gas phase by electron diffraction and vibrational spectroscopy (IR and Raman). The experimental investigations were supplemented by ab initio (MP2) and DFT quantum chemical calculations at different levels of theory. Theoretical methods reveal two structures with Cs (anti, anti) and C1 (anti, gauche) symmetries. The infrared and Raman spectra for different phases were also recorded and the bands observed assigned to the vibrational normal modes. Liquid Raman and infrared spectra in liquid and gaseous state measurements revealed the presence of two conformations anti, anti (Cs symmetry) and anti, gauche (C1 symmetry). The study was completed using natural bond orbital (NBO) analysis. We have also analyzed the internal rotation barrier about the C(O)SCC dihedral angle using a variety of computational approaches and natural bond orbital (NBO) analyses to understand the nature of the potential function and to explain the preferred conformation of the molecule.

Transmissão e a influência do volume dos estoques públicos sobre o preço do arroz no Brasil

This study examined the mechanism of prices from major rice producing states in Brazil (Rio Grande do Sul, Mato Grosso and Santa Catarina), as endogenous variables, and volume of public stocks in Brazil, as exogenous variables for the period July 2004 until December 2010. The analysis includes the use of VAR-VEC methodology to identify the number of lags used and check for co-integration among variables through the test of Johansen Co-integration. We tested the existence of causality between variables by the method of Granger causality. We applied the variance decomposition of forecast error and impulse-response function with Cholesky decomposition to analyze the relationship between prices and volume of the states in public stocks. With the proposed methodology, one can observe that the price of rice in the state of Rio Grande do Sul to the price in the state of Santa Catarina and the volume stored in stockpiles and is influenced by the price of rice in the state of Mato Grosso.

Este trabalho analisou o mecanismo de transmissão dos preços dos principais estados produtores de arroz no Brasil (Rio Grande do Sul, Mato Grosso e Santa Catarina), como variáveis endógenas, e volume dos estoques públicos no Brasil, como variável exógena, para o período de julho de 2004 até dezembro de 2010. A análise compreende o uso da metodologia VAR-VEC para identificar o número de defasagens utilizadas e verificar se existem co-integrações entre as variáveis, por meio do Teste de Co-integração de Johansen. Testou-se a existência de causalidade entre as séries pelo método de causalidade de Granger. Aplicou-se a decomposição da variância do erro de previsão e a função impulso-resposta com decomposição de Cholesky para analisar a relação entre os preços dos estados e volume em estoques públicos. Com a aplicação da metodologia proposta, pode-se observar que o preço do arroz no estado do Rio Grande do Sul influencia no preço no estado de Santa Catarina e no volume armazenado em estoques públicos e é influenciado pelo preço do arroz no estado do Mato Grosso.

Transmissão e a influência do volume dos estoques públicos sobre o preço do arroz no Brasil

This study examined the mechanism of prices from major rice producing states in Brazil (Rio Grande do Sul, Mato Grosso and Santa Catarina), as endogenous variables, and volume of public stocks in Brazil, as exogenous variables for the period July 2004 until December 2010. The analysis includes the use of VAR-VEC methodology to identify the number of lags used and check for co-integration among variables through the test of Johansen Co-integration. We tested the existence of causality between variables by the method of Granger causality. We applied the variance decomposition of forecast error and impulse-response function with Cholesky decomposition to analyze the relationship between prices and volume of the states in public stocks. With the proposed methodology, one can observe that the price of rice in the state of Rio Grande do Sul to the price in the state of Santa Catarina and the volume stored in stockpiles and is influenced by the price of rice in the state of Mato Grosso.

Este trabalho analisou o mecanismo de transmissão dos preços dos principais estados produtores de arroz no Brasil (Rio Grande do Sul, Mato Grosso e Santa Catarina), como variáveis endógenas, e volume dos estoques públicos no Brasil, como variável exógena, para o período de julho de 2004 até dezembro de 2010. A análise compreende o uso da metodologia VAR-VEC para identificar o número de defasagens utilizadas e verificar se existem co-integrações entre as variáveis, por meio do Teste de Co-integração de Johansen. Testou-se a existência de causalidade entre as séries pelo método de causalidade de Granger. Aplicou-se a decomposição da variância do erro de previsão e a função impulso-resposta com decomposição de Cholesky para analisar a relação entre os preços dos estados e volume em estoques públicos. Com a aplicação da metodologia proposta, pode-se observar que o preço do arroz no estado do Rio Grande do Sul influencia no preço no estado de Santa Catarina e no volume armazenado em estoques públicos e é influenciado pelo preço do arroz no estado do Mato Grosso.

THE EVALUATION OF PEPTIDE/HISTIDINE TRANSPORTER 1 (PHT1) FUNCTION: UPTAKE KINETICS UTILIZING A COS-7 STABLY TRANSFECTED CELL LINE.

There have been relatively few studies focused on the proton-dependent oligopeptide transporter (POT) superfamily member, Peptide/Histidine Transporter 1 (PHT1), with respect to its contribution to the ADME of peptides and peptide-based drugs. These studies were conducted to determine hPHT1-mediated, H+-dependent uptake kinetics of histidine, carnosine, Gly-Sar and valacyclovir in stably transfected hPHT1-COS-7 cells comparative to kinetics determined in an empty vector (Mock) stably transfected cell line. The results suggest that Gly-Sar appears to be a substrate for PHT1 based on efflux from the stably transfected hPHT1 COS-7 cells. Histidine and Gly-Sar concentration- and time-dependent studies suggest mixed-uptake kinetics. These studies suggest that stably transfected hPHT1-COS-7 cells exhibit different uptake kinetics than those observed in our previous studies and illustrate the requirement for experiments to delineate the physiological role of hPHT1.

Regionalização sob a ótica dos gestores: uma abordagem dialética / Regionalization as seen by managers: a dialectic approach

Este estudo é parte da pesquisa interdisciplinar Estudo da Regionalização e Organização da Assistência de Média e Alta Complexidade na Macrorregião Sudeste de Minas Gerais realizada por pesquisadores da Universidade Federal de Juiz de Fora (UFJF) e da Prefeitura de Juiz de Fora e alunos de iniciação científica da UFJF, concluída em 2008. Serão apresentados os dados e as reflexões relacionadas à organização da assistência à saúde do Sistema Único de Saúde na Macrorregião Sudeste de Minas Gerais que foram obtidos junto aos gestores municipais e ao Hospital Universitário da UFJF. Neste artigo, analisamos a regulação do acesso dos usuários à rede de saúde do município de Juiz de Fora. A preocupação principal é identificar como se estabelecem as relações entre os gestores dos municípios limítrofes com o município de Juiz de Fora, polo macrorregional de atenção à saúde e o Hospital Universitário da UFJF. A abordagem dialética formou a base metodológica deste estudo, considerando as categorias: contradição, totalidade e historicidade.

This study is part of the interdisciplinary study, Regionalization and Organization of Medium and High--Complexity Care in the Southeast Macro-region of Minas Gerais, undertaken by researchers and students from the Federal University of Juiz de Fora and researchers from the City Council, and concluded in 2008. We present data and reflections related to health care organization in the Brazilian Unified Health System (SUS) of the Southeast macro-region of Minas Gerais, which were obtained from the municipal managers and the Federal University of Juiz de Fora Hospital (HU-UFJF). In this article, we analyze the regulation of users` access to the health care network of the Juiz de Fora municipality. The main aim was to identify how the relationships between managers from neighboring municipalities and the HU-UFJF are built. The dialectic approach was the method used in this study, with the following categories being considered: contradiction, totality and historicity.

Acesso e regionalização em saúde: análise de um serviço ambulatorial de média complexidade / Access and regionalization in health: analysis of a medium complexity walk-in care service

Este artigo avalia o Serviço de Doenças Intestinais Inflamatórias do Hospital Universitário da Universidade Federal de Juiz de Fora, responsável pelo tratamento dos pacientes portadores de doença de Crohn ou de retocolite ulcerativa, em relação às normas que determinam a regionalização da assistência à saúde para identificar os limites e possibilidades do acesso e a caracterização dos usuários que, em face às peculiaridades da doença, são orientados para o atendimento fora dos padrões regulamentados no Plano Diretor de Regionalização do Estado de Minas Gerais. A situação diferenciada é o foco deste estudo, considerando-se o processo de contratualização entre a Secretaria Municipal de Saúde e o Hospital Universitário.

This article assesses the Inflammatory Intestinal Diseases Unit of the Federal University of Juiz de Fora Hospital, which is responsible for the care of patients with Crohn`s disease or ulcerative rectocolitis, as concerns adherence to the norms regulating the regionalization of health care, in order to identify access limitations and possibilities, and to characterize users who, owing to the peculiarities of the aforementioned diseases, are advised to seek care beyond the patterns standardized by the Regionalization Management Plan of the Minas Gerais state. This particular situation, taking into account the agreement process between the Municipal Health Authority and the University Hospital, is the focus of the study.

Humic substances isolated from residues of sugar cane industry as root growth promoter

Plant growth promoting substances are widely used in modern agriculture. Several products in the market are humic substances isolated from different sources. The filter cake, a residue of sugar production, is a rich and renewable source of organic matter and these characteristics place the filter cake as a possible source of plant growth promoting substances. Humic acids (HA) from filter cake were characterized, and their effects as root growth promoters were evaluated. Chemical features of the HA were evaluated through elemental composition, acidic functional groups, E4/E6 ratio and infrared spectroscopy analyzes. The biological activity of the HA was assessed using root architecture parameters and the P-type H+-ATPase activity. The lateral root development was directly related to the stimulation of plasma membrane ATPase activity. The ability of HA to promote root development indicate that HA from filter cake can be used as environmental plant growth stimulators.

Substâncias promotoras do crescimento vegetal são amplamente utilizadas na agricultura moderna. Existem vários produtos no mercado, muitos dos quais são substâncias húmicas isoladas de diferentes fontes. A torta de filtro, um resíduo da produção do açúcar, é uma fonte rica e renovável de matéria orgânica e essas características a tornam uma possível fonte de substâncias promotoras do crescimento vegetal. Ácidos húmicos (AH) da torta de filtro foram caracterizados, e foi avaliado seu efeito como promotor de crescimento radicular. As características químicas dos AH foram avaliadas por meio da composição elementar, grupos funcionais ácidos, relação E4/E6 e espectroscopia de infravermelho. A atividade biológica dos AH foi acessada avaliando-se a arquitetura radicular e a atividade da H+-ATPase de membrana plasmática. O desenvolvimento de raízes laterais foi diretamente relacionado ao estímulo da atividade da H+-ATPase. A habilidade dos AH em promover o desenvolvimento radicular indica que AH extraídos da torta de filtro podem ser utilizados como estimuladores do crescimento de plantas.

Humic substances isolated from residues of sugar cane industry as root growth promoter

Plant growth promoting substances are widely used in modern agriculture. Several products in the market are humic substances isolated from different sources. The filter cake, a residue of sugar production, is a rich and renewable source of organic matter and these characteristics place the filter cake as a possible source of plant growth promoting substances. Humic acids (HA) from filter cake were characterized, and their effects as root growth promoters were evaluated. Chemical features of the HA were evaluated through elemental composition, acidic functional groups, E4/E6 ratio and infrared spectroscopy analyzes. The biological activity of the HA was assessed using root architecture parameters and the P-type H+-ATPase activity. The lateral root development was directly related to the stimulation of plasma membrane ATPase activity. The ability of HA to promote root development indicate that HA from filter cake can be used as environmental plant growth stimulators.

Substâncias promotoras do crescimento vegetal são amplamente utilizadas na agricultura moderna. Existem vários produtos no mercado, muitos dos quais são substâncias húmicas isoladas de diferentes fontes. A torta de filtro, um resíduo da produção do açúcar, é uma fonte rica e renovável de matéria orgânica e essas características a tornam uma possível fonte de substâncias promotoras do crescimento vegetal. Ácidos húmicos (AH) da torta de filtro foram caracterizados, e foi avaliado seu efeito como promotor de crescimento radicular. As características químicas dos AH foram avaliadas por meio da composição elementar, grupos funcionais ácidos, relação E4/E6 e espectroscopia de infravermelho. A atividade biológica dos AH foi acessada avaliando-se a arquitetura radicular e a atividade da H+-ATPase de membrana plasmática. O desenvolvimento de raízes laterais foi diretamente relacionado ao estímulo da atividade da H+-ATPase. A habilidade dos AH em promover o desenvolvimento radicular indica que AH extraídos da torta de filtro podem ser utilizados como estimuladores do crescimento de plantas.

Ação de produtos naturais sobre a sobrevivência de Argyrotaenia sphaleropa (Meyrick) (Lepidoptera: Tortricidae) e seletividade de inseticidas utilizados na produção orgânica de videira sobre Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae)

This work was conducted to study the effect of commercial formulations of insecticides with emphasis on that allowed in the organic production (neem, natural piretro and pirolenhoso extract) to control Argyrotaenia sphaleropa (Meyrick) (Lepidoptera: Tortricidae) and their performance on the egg parasitoid Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) adults. The insecticides Natuneem® (1500ppm of azadirachtin L-1) and the pirolenhoso extract (Biopirol 7 M®) (250 and 500mL 100L-1) were not efficient in the control of A. sphaleropa when applied over grapevine leaves (Vitis sp.) cultivate Chardonnay. Natural piretro (250 and 500mL 100L-1) resulted in a mortality of 77.65 and 85.88% of insects, respectively 120 hours after application. The secondary effect of insecticides allowed was evaluated on adults of the egg parasitoid T. pretiosum following International Organization for Biological and Integrated Control (IOBC) methodology. It was observed that Natuneem® (500mL 100L-1), Biopirol 7 M® (500mL 100L-1) and Dipel DF® (100g 100L-1) had been innocuous ( 30% parasitism reduction) while natural piretro (250mL 100L-1) was classified as moderately harmful (80-99% of parasitism reduction). Natural piretro in the higher dose (500mL 100L-1) showed a parasitism reduction in more than 99% in the same way that the pattern Lebaycid 500® (100mL 100 L-1).

Nesse trabalho, foi estudado o efeito de formulações comerciais de inseticidas, com ênfase para os produtos permitidos na produção orgânica (nim, piretro natural e extrato pirolenhoso) para o controle de Argyrotaenia sphaleropa (Meyrick) (Lepidoptera: Tortricidae) e sua atuação sobre o parasitóide de ovos Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae), em laboratório. Os inseticidas Natuneem® (1500ppm de Azadirachta indica por litro) e o extrato pirolenhoso (Biopirol 7 M®), nas dosagens de 250 e 500mL 100L-1, não foram eficientes no controle de A. sphaleropa quando aplicados sobre folhas de videira (Vitis sp.) cultivar "Chardonnay", enquanto que o piretro natural (250 e 500mL 100L-1) resultou em mortalidade significativa de 77,65 e 85,88% dos insetos, respectivamente, 120 horas após a aplicação. O efeito secundário foi avaliado sobre adultos do parasitóide de ovos T. pretiosum, seguindo a metodologia da International Organization for Biological and Integrated Control (IOBC). Os inseticidas Natuneem® (500mL 100L-1), Biopirol 7 M® (500mL 100L-1) e Dipel DF® (100g 100L-1) foram inócuos ( 30% de redução no parasitismo por T. pretiosum), enquanto o piretro natural, nas dosagens de 250mL 100L-1 e 500mL 100 L-1, foi classificado como moderadamente nocivo (80-99% de redução no parasitismo) e nocivo (>99% de redução no parasitismo), respectivamente, equivalendo-se ao efeito do fosforado Lebaycid 500® (100mL 100 L-1).

Ação de produtos naturais sobre a sobrevivência de Argyrotaenia sphaleropa (Meyrick) (Lepidoptera: Tortricidae) e seletividade de inseticidas utilizados na produção orgânica de videira sobre Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae)

This work was conducted to study the effect of commercial formulations of insecticides with emphasis on that allowed in the organic production (neem, natural piretro and pirolenhoso extract) to control Argyrotaenia sphaleropa (Meyrick) (Lepidoptera: Tortricidae) and their performance on the egg parasitoid Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) adults. The insecticides Natuneem® (1500ppm of azadirachtin L-1) and the pirolenhoso extract (Biopirol 7 M®) (250 and 500mL 100L-1) were not efficient in the control of A. sphaleropa when applied over grapevine leaves (Vitis sp.) cultivate Chardonnay. Natural piretro (250 and 500mL 100L-1) resulted in a mortality of 77.65 and 85.88% of insects, respectively 120 hours after application. The secondary effect of insecticides allowed was evaluated on adults of the egg parasitoid T. pretiosum following International Organization for Biological and Integrated Control (IOBC) methodology. It was observed that Natuneem® (500mL 100L-1), Biopirol 7 M® (500mL 100L-1) and Dipel DF® (100g 100L-1) had been innocuous ( 30% parasitism reduction) while natural piretro (250mL 100L-1) was classified as moderately harmful (80-99% of parasitism reduction). Natural piretro in the higher dose (500mL 100L-1) showed a parasitism reduction in more than 99% in the same way that the pattern Lebaycid 500® (100mL 100 L-1).

Nesse trabalho, foi estudado o efeito de formulações comerciais de inseticidas, com ênfase para os produtos permitidos na produção orgânica (nim, piretro natural e extrato pirolenhoso) para o controle de Argyrotaenia sphaleropa (Meyrick) (Lepidoptera: Tortricidae) e sua atuação sobre o parasitóide de ovos Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae), em laboratório. Os inseticidas Natuneem® (1500ppm de Azadirachta indica por litro) e o extrato pirolenhoso (Biopirol 7 M®), nas dosagens de 250 e 500mL 100L-1, não foram eficientes no controle de A. sphaleropa quando aplicados sobre folhas de videira (Vitis sp.) cultivar "Chardonnay", enquanto que o piretro natural (250 e 500mL 100L-1) resultou em mortalidade significativa de 77,65 e 85,88% dos insetos, respectivamente, 120 horas após a aplicação. O efeito secundário foi avaliado sobre adultos do parasitóide de ovos T. pretiosum, seguindo a metodologia da International Organization for Biological and Integrated Control (IOBC). Os inseticidas Natuneem® (500mL 100L-1), Biopirol 7 M® (500mL 100L-1) e Dipel DF® (100g 100L-1) foram inócuos ( 30% de redução no parasitismo por T. pretiosum), enquanto o piretro natural, nas dosagens de 250mL 100L-1 e 500mL 100 L-1, foi classificado como moderadamente nocivo (80-99% de redução no parasitismo) e nocivo (>99% de redução no parasitismo), respectivamente, equivalendo-se ao efeito do fosforado Lebaycid 500® (100mL 100 L-1).

Effects of the adiabatic evaporative cooling system in free-stall on physiological responses in dairy cows / Efeitos do sistema de resfriamento adiabático e evaporativo em free-stall sobre as resposatas fisiológicas de vacas em lactação

The purpose of this research was to compare the efficiency of three different schedules using an adiabatic evaporative cooling with misting system (AEC) in a free-stall and its influence on the physiological parameters on lactating cows. A total of 28 Brown Swiss and Holstein multiparous cows were random allocated in four groups. The overall experimental period was of 56 days, starting from January 20th. Treatments were: control group (without AEC); Day (AEC turned on from 7 to 19 h); Night (AEC turned on from 19 to 7 h) and 24 h (AEC turned on during 24h). The dry bulb temperature (DBT), relative humidity (RH) and black globe temperature (BGT) were measuread hourly inside and outside of facilities through a period of 24 hours. The respiratory frequency (RF), rectal temperature (RT) and skin temperature (ST) were registered twice a week at 7, 13 e 21 h. It was observed a positive linear correlation between rectal temperature and ST. The free-stall cooling used in the day group kept the temperature under the thermoneutrality range till 12 h, however from 17 h in the day group the DBT was statistically higher than control and 24 h groups.

O trabalho teve como objetivo avaliar a eficiência da climatização por nebulização, em instalação do tipo free-stall, acionada em diferentes horários, por intermédio da temperatura de bulbo seco (TBS) e umidade relativa do ar (UR) e sua influência nos parâmetros fisiológicos, temperatura retal (TR), freqüência respiratória (FR) e temperatura do pelame (TP). Foram utilizadas 28 vacas, multíparas, com produção média diária de 23 kg de leite, com 60 dias de paridas, distribuídas em delineamento inteiramente casualisado. O experimento foi realizado no verão e teve duração de 56 dias. Os tratamentos foram: controle (sem climatização); Dia (climatizado das 7-19h); Noite (climatizado das 19-7h) e 24 horas (climatizado 24h). Dentro do free-stall, em cada tratamento, foram tomadas medidas de temperatura e umidade relativa do ar, com um registrador digital automático, durante período de 24 h a cada 60 minutos. A temperatura retal (TR), a freqüência respiratória (FR) e a temperatura do pelame (TP) foi obtida duas vezes por semana às 7, 13 e 21 h de três animais de cada tratamento. Foi observada uma correlação linear positiva entre a temperatura retal e as temperaturas do pelame. A climatização do free-stall utilizado no tratamento dia conseguiu manter a temperatura dentro da faixa de termoneutralidade até as 12 h, mas a partir da 17 h a TBS do tratamento dia foi significativamente maior q

Effects of the adiabatic evaporative cooling system in free-stall on physiological responses in dairy cows / Efeitos do sistema de resfriamento adiabático e evaporativo em free-stall sobre as resposatas fisiológicas de vacas em lactação

The purpose of this research was to compare the efficiency of three different schedules using an adiabatic evaporative cooling with misting system (AEC) in a free-stall and its influence on the physiological parameters on lactating cows. A total of 28 Brown Swiss and Holstein multiparous cows were random allocated in four groups. The overall experimental period was of 56 days, starting from January 20th. Treatments were: control group (without AEC); Day (AEC turned on from 7 to 19 h); Night (AEC turned on from 19 to 7 h) and 24 h (AEC turned on during 24h). The dry bulb temperature (DBT), relative humidity (RH) and black globe temperature (BGT) were measuread hourly inside and outside of facilities through a period of 24 hours. The respiratory frequency (RF), rectal temperature (RT) and skin temperature (ST) were registered twice a week at 7, 13 e 21 h. It was observed a positive linear correlation between rectal temperature and ST. The free-stall cooling used in the day group kept the temperature under the thermoneutrality range till 12 h, however from 17 h in the day group the DBT was statistically higher than control and 24 h groups.

O trabalho teve como objetivo avaliar a eficiência da climatização por nebulização, em instalação do tipo free-stall, acionada em diferentes horários, por intermédio da temperatura de bulbo seco (TBS) e umidade relativa do ar (UR) e sua influência nos parâmetros fisiológicos, temperatura retal (TR), freqüência respiratória (FR) e temperatura do pelame (TP). Foram utilizadas 28 vacas, multíparas, com produção média diária de 23 kg de leite, com 60 dias de paridas, distribuídas em delineamento inteiramente casualisado. O experimento foi realizado no verão e teve duração de 56 dias. Os tratamentos foram: controle (sem climatização); Dia (climatizado das 7-19h); Noite (climatizado das 19-7h) e 24 horas (climatizado 24h). Dentro do free-stall, em cada tratamento, foram tomadas medidas de temperatura e umidade relativa do ar, com um registrador digital automático, durante período de 24 h a cada 60 minutos. A temperatura retal (TR), a freqüência respiratória (FR) e a temperatura do pelame (TP) foi obtida duas vezes por semana às 7, 13 e 21 h de três animais de cada tratamento. Foi observada uma correlação linear positiva entre a temperatura retal e as temperaturas do pelame. A climatização do free-stall utilizado no tratamento dia conseguiu manter a temperatura dentro da faixa de termoneutralidade até as 12 h, mas a partir da 17 h a TBS do tratamento dia foi significativamente maior q