Effect of low-glucosinolate crambe meal in diet on lactational performance, efficiency of nutrient utilization, and hepatic function of crossbred Holstein × Zebu and Jersey cows.

Crambe meal (CM) is a potential dietary protein source for ruminant, but its effects in diets for lactating dairy cows remains unknown. We evaluated the effects of inclusion of the low-glucosinolates (GIs) CM (450 mg GIs/kg DM) in partial total mixed ration (pTMR) on performance, efficiency of nutrient utilization, and hepatic function of crossbred Holstein × Zebu and Jersey cows. Eight crossbred Holstein × Zebu cows and four Jersey cows were blocked by breed and days in milk, and randomly assigned in a replicated 4 × 4 Latin square design, and distributed in one of four isonitrogenous TMRs (130 g CP/kg DM): 0, 45, 90, and 135 g CM/kg DM pTMR. Crambe meal was included in pTMR replacing soybean meal (SBM) and ground corn grain (GCG). The pTMRs were offered ad libitum between 7:00 a.m. and 5:00 p.m. Cows were kept on pasture of Panicum maximum cv. Mombaça (90.4 g CP/kg DM) between 6:00 p.m. and 6:30 a.m. Pasture intake was measured from external and internal fecal excretion marker. Inclusion of CM did not affect (P ≤ 0.05) the DM intakes of pTMR and pasture, apparent total-tract digestibilities of organic matter, CP and NDF, ruminal outflow microbial protein, milk yield, milk composition, urinary N excretion, milk N efficiency, and level of plasm hepatic enzymes. The effects of CM inclusion in pTMR were independent of breed. Low-glucosinolates CM can be incorporated up to 135 g/kg DM in pTMR in replacement of SBM and GCG without affecting performance, efficiency of nutrient utilization, and hepatic function of crossbred Holstein × Zebu and Jersey cows.

Nutritional evaluation of crambe meal as a partial replacement of soybean meal in Nile tilapia diet.

A variety of plant protein sources have been evaluated in aquafeeds. Crambe meal (CM) has potential for inclusion in fish diets because of its nutritional composition. This study evaluated the apparent digestibility coefficient (ADC) of crambe meal and its potential to partially replace soybean meal (SM) protein in Nile tilapia Oreochromis niloticus diets. The ADC for dry matter, crude protein, ether extract, energy, amino acids, calcium and phosphorus of CM were assessed in fish (n = 80; 65.30 ± 5.32 g). Subsequently, an 80-day feeding trial was conducted with Nile tilapia (n = 140; 6.04 ± 0.25 g) randomly distributed in 20 experimental cages (70 L; seven fish cage-1) allocated in five circular tanks (1000 L) in a recirculation water system, to evaluate the effects of replacement of SM by CM (0, 6, 12, 18 and 24% in isonitrogenous and isoenergetic diets) on growth, blood parameters, fillet yield and proximal composition. The CM shows good digestibility of protein (0.824) and amino acids (0.844) by Nile tilapia and its inclusion in the diet does not affect carcass and fillet yield or proximal composition. Fish fed diets with 24.0% of the SM replaced by CM showed the worst weight gain and feed conversion rate. The protein efficiency ratio decreased in fish fed diets with 12.0, 18.0 and 24.0% of the SM replaced by CM. Hemoglobin, mean corpuscular hemoglobin concentration, total plasma protein, glucose and alanine aminotransferase enzyme activity trend to increase at highest levels of CM in the diet. In conclusion, CM has high digestibility of protein and amino acids for Nile tilapia. However, anti-nutritional factors present in untreated CM interfere on the growth and nutrient utilization of Nile tilapia.

Crambe meal and crude glycerin from biodiesel production in diets for finishing of crossbred cattle in feedlot.

Our hypothesis is that crambe meal can totally substitute soybean meal and crude glycerin can partially replace the corn in the diet without leading to losses of nutrient intake and productive performance. Two assays were performed using heifers and steers. In the first assay, 24 Beefalo-Nellore heifers with 18 months old with 311.5 ± 5.5 kg, and in the second assay, 24 Beefalo-Nellore steers with 18 months old with 317.0 ± 6.4 kg were evaluated. The experiment was carried out in a randomized complete design, in a 2 × 2 factorial scheme, consisting of two energy sources (corn and crude glycerin) and two protein sources (soybean meal and crambe meal). Both assays had 104 experimental days of feedlot. There was no interaction between the variation sources. There was an effect of protein source on nutrient intake for heifers except to neutral detergent fiber intake, which presented effect of energy source. Heifers fed soybean meal showed higher results for total weight gain and daily weight gain. There was observed effect of energy source on rib eye area and subcutaneous fat thickness. Steers fed soybean meal showed higher neutral detergent fiber intake and non-fibrous carbohydrates. Steers fed corn + crude glycerin showed higher results for total weight gain and daily weight gain. The steers fed soybean meal showed higher averages for subcutaneous fat thickness. Crambe meal and crude glycerin can be used as alternative sources of energy and protein to replace 100% of soybean and 50% of corn, respectively, for finishing Beefalo-Nellore cattle in a feedlot.

Acaricidal Properties of Bio-Oil Derived From Slow Pyrolysis of Crambe abyssinica Fruit Against the Cattle Tick Rhipicephalus microplus (Acari: Ixodidae).

Slow pyrolysis is a process for the thermochemical conversion of biomasses into bio-oils that may contain a rich chemical composition with biotechnological potential. Bio-oil produced from crambe fruits was investigated as to their acaricidal effect. Slow pyrolysis of crambe fruits was performed in a batch reactor at 400°C and chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). The bio-oil collected was used in bioassays with larvae and engorged females of the cattle tick Rhipicephalus microplus. Biological assays were performed using the larval packet test (LPT) and adult immersion test. The GC-MS of crambe fruit bio-oil revealed mainly hydrocarbons such as alkanes and alkenes, phenols, and aldehydes. The bio-oil in the LPT exhibited an LC90 of 14.4%. In addition, crambe bio-oil caused female mortality of 91.1% at a concentration of 15% and a high egg-laying inhibition. After ovary dissection of treated females, a significant reduction in gonadosomatic index was observed, indicating that bio-oil interfered in tick oogenesis. Considering these results, it may be concluded that slow pyrolysis of crambe fruit affords a sustainable and eco-friendly product for the control of cattle tick R. microplus.

Combinations of by-products from biodiesel production included in the supplement for finishing heifers on deferred pastures.

We hypothesized that by-products from biodiesel production like crambe meal (Crambe abyssinica) totally replace soybean meal (Glycine max) and crude glycerin could partially replace corn (Zea mays). Our aim was to evaluate different supplements with combinations of crambe meal and crude glycerin replacing soybean meal or corn on performance and carcass traits of finishing heifers. Sixty Nellore heifers 18 months old were used, and 298.96 ± 21.99 kg of body weight allotted in Brachiaria brizantha pastures. All heifers were distributed in a randomized complete block design, with four treatments being the following supplements: corn + soybean meal (CS), corn + crambe meal (CCr), corn + soybean meal + crude glycerin (CSG), corn + crambe meal + crude glycerin + (CCrG). The animals were distributed in four paddocks of 13 hectares each, in a continuous grazing system. We evaluated pasture characteristics, intake, performance, carcass traits, characteristics, and centesimal composition of Longissimus thoracis muscle. There was an effect (P < 0.05) of inclusion of crambe meal on the performance of the animals for slaughter weight and daily gain. The supplement containing corn + soybean meal (CS) presented better conversion rates and food efficiency. There was an effect of inclusion of crambe meal and crude glycerin on the centesimal composition of Longissimus thoracis muscle. The use of crambe meal and crude glycerin as alternative sources from the agro-industry of biodiesel in concentrate supplement for finishing heifers in pastures can promote positive effects related to productive performance and carcass characteristics.

Production components of crambe in paraiba semi-arid region with nitrogen fertilization and different spacings / Componentes de produção de crambe no semiárido paraibano com adubação nitrogenada e diferentes espaçamentos

Crambe (Crambe abyssinica H.), a promising species in the production of biodiesel, has low planting cost, high oil content and easy extraction, besides being an alternative for the crop rotation system. The objective of this study was to evaluate the effects of nitrogen fertilization and line spacing on the production components and yield of the crambe crop under semi-arid conditions. The study was conducted under field conditions in the experimental area of the National Semi-Arid Institute-INSA, located in the city of Campina Grande-PB. The experimental design was a randomized complete block design in 4 x 2 factorial scheme, consisting of four nitrogen doses (30, 60, 90, 120 kg ha-1) with two spacings between planting lines (0.20 and 0.40 meters) with three replications, totaling 24 experimental plots. The evaluated parameters were: number of siliques per plant, dry weight of shoots, number of branches (primary and secondary), primary stem length, number of siliques per stem, mass of 1000 grains and productivity. The data were submitted to analysis of variance, in which the nitrogen doses (quantitative factor) were compared by regression analysis. The different spacings were compared by means of a t-test at the 0.05 probability level by using statistical software SISVAR version 5.2. Nitrogen fertilization at 0.40 m spacing between the planting lines led to the best results for the production components, and the highest productivity was obtained at 0.20 m spacing of with nitrogen fertilization of 120 kg ha-1.

O crambe (Crambeabyssinica H.), espécie promissora na produção de biodiesel, apresenta baixo custo de plantio, elevado teor de óleo e de fácil extração, além de ser alternativa para o sistema de rotação de culturas. Mediante a necessidade de conhecimento para ampliação do cultivo dessa espécie, este estudo teve como objetivo avaliar os efeitos da adubação nitrogenada e espaçamento entre linhas no cultivo nos componentes de produção e na produtividade da cultura do crambe em condições semiáridas. O estudo foi conduzido em condições de campo na área experimental pertencente ao Instituto Nacional do Semiárido-INSA, situado no município de Campina Grande-PB. O delineamento experimental utilizado foi em blocos casualizados, no esquema fatorial 4 x 2, sendo constituído de quatro doses de nitrogênio (30; 60; 90; 120 kg ha-1) com dois espaçamentos entre linhas de plantio (0,20 e 0,40 metros) e com três repetições, totalizando 24 parcelas experimentais. Os parâmetros avaliados foram: número de síliquas por planta, massa seca da parte aérea, número de ramificações (primárias e secundárias), comprimento de haste primária, número de silíquas por haste, massa de 1000 grãos e produtividade. Os dados obtidos foram submetidos à análise de variância, em que as doses de nitrogênio (fator quantitativo) foram comparadas por análise de regressão. Os diferentes espaçamentos foram comparados por meio de comparação de médias, pelo teste t, ao nível de 0,05 de probabilidade utilizando-se software estatístico SISVAR versão 5.2. A adubação nitrogenada sob espaçamento de 0,40 m, entre as linhas de plantio, proporcionou os melhores resultados quanto aos componentes de produção, e a maior produtividade foi obtida no espaçamento referente a 0,20 m com a adubação nitrogenada de 120 kg ha-1.

Phytoremediation capacity, growth and physiological responses of Crambe abyssinica Hochst on soil contaminated with Cd and Pb.

The search for vegetal species regarding effectiveness in the phytoremediation of soils is of great importance, mainly in function of the great environmental problems, such as soil contamination with heavy metals, the necessity of producing more food, among others that mankind face today. This work aimed (i) to evaluate phytoremediation capacity of Crambe abyssinica Hochst and its growth in soil artificially contaminated with Cd and Pb, and (ii) to evaluate the possible impacts of crambe cultivation in contaminated soil conditions, in order to evaluate, to test, and to question the Brazilian CONAMA 420, providing important information that can be useful for governmental and environmental purposes. Two simultaneous experiments were developed, one for each metal. The soils were contaminated with salts of CdCl2 and PbCl2H2O in five doses based on the investigation values (IV) of CONAMA Resolution 420, resulting in 0; 1.5; 3; 9 and 30 mg kg-1 for Cd and 33; 90; 180; 540 and 1800 mg kg-1 for Pb. Gaseous exchange, development, nutritional composition and production of plant components, as well as phytoavailability of metals, were evaluated. The contamination with metals reduced photosynthesis, increased breathing as well as leading to a negative effect on the mineral nutrition and productivity in general; Plants cultivated in soil with Cd presented higher phytoavailability when compared to those cultivated in the Pb conditions, being found metals in all parts of the crambe plants from 1.5 mg kg- 1 of Cd in the soil; and Pb was retained only in roots, not being translocated in the plant. Cd showed higher phytoavailability, being found in all parts of the plant and Pb was retained only in the roots. Cd showed a higher phytoavailability when compared to Pb, also being found in all parts of crambe plants from dose 1.5 mg kg-1 of Cd in soil, which is an environmental problem, since in these concentrations the cultivation of crops is allowed by Brazilian legislation CONAMA 420.

Intake, digestibility and recovery of grains from feces of steers fed different oilseeds / Consumo, digestibilidade e recuperação de grãos nas fezes, de novilhos alimentados com diferentes grãos de oleaginosas

Oilseed grains are used as a protein and energy source, increasing the energy density of the feed. The goal of the present study was to determine the nutritional profile of canola, crambe and soybean grains recovered from feces, and to evaluate the intake and digestibility of nutrients, rumen fermentation parameters of beef cattle receiving whole grains of oilseeds. Three steers, 350 ± 23.5 kg body weight were distributed in a not contemporary, 3 × 3 double Latin square, and fed oilseed grains. Inclusion of crambe grain reduced dry matter intake by 21.02%, with a higher digestibility coefficient for EE and NDF. The crude protein content of canola and crambe grains recovered were similar, but the EE content was higher for the crambe grain (26.46%). Soybean showed the lowest values of chemical composition for grains recovered from feces. The highest dry matter intake (7.86 kg/day), and the lowest NDF digestibility (0.203g/kg), were found for animals fed soybean. The inclusion of crambe grains reduced the rumen ammonia content (10.97 mg/dL); however, the ruminal pH was not altered by the inclusion of grains in the diet. Canola and soybean grains can be included in the cattle diet without altering dry matter intake; while the inclusion of crambe grains reduces the dry matter intake and the ruminal ammonia content of the animals. Crambe grain showed the highest recovery of dry matter and ether extract from feces.

Para se avaliar a inclusão de grãos inteiros das culturas de canola, crambe e soja na dieta de bovinos em confinamento, sobre o consumo, digestibilidade, parâmetros ruminais e recuperação dos grãos nas fezes, delineou-se um ensaio estatístico em quadrado latino com três dietas e três bovinos providos de cânula ruminal. Os resultados constataram que a inclusão do grão de crambe reduziu o consumo de matéria seca em 21,02%, e proporcionou aumento na digestibilidade do extrato etéreo e fibra em detergente neutra. O percentual de extrato etéreo recuperado nas fezes foi de 44,15% para grão de canola e 61,53% para o grão de crambe. Encontrou-se menor digestibilidade da fibra em detergente neutra para dietas contendo grão de soja 0,203g/kg, embora seu consumo aumentou em relação ao crambe 7,86 kg/dia. A inclusão de crambe apresentou redução nos teores de amônia ruminal (N-NH3), com valores médios de 10,97 mg/dL. Portanto, o grão de crambe pode ser incluído na dieta de bovinos de corte em 14% da MS, sendo uma alternativa em potencial como grão de oleaginosa na alimentação de ruminantes. Assim como, os grãos de canola e soja sem afetar a digestibilidade da matéria seca e da proteína bruta, contudo, devem ser usados com cautela.

Analysis of 1H NMR spectra of diesel and crambe biodiesel mixtures using chemometrics tools to evaluate the authenticity of a Brazilian standard biodiesel blend.

A methodology was developed to monitor the content of crambe biodiesel in mixtures with conventional diesel using hydrogen nuclear magnetic resonance (1H NMR) spectroscopy combined with the orthogonal projections on the latent structure-discrimination analysis (OPLS-DA). The efficiency of the developed OPLS-DA model was analyzed based on the criteria of true response statistics: false positive and false negative rate, sensitivity, specificity, efficiency and Matthew's correlation coefficient, where the sensitivity (true positive rate) and specificity (true negative rate) were both equal to 1 and the false positive and false negative rates were both equal to 0, which means that all samples to be predicted as belonging to the diesel class of interest, B10 (containing 10% biodiesel and 90% pure diesel), were predicted in class 1, and all samples to be considered as belonging to the diesel class, not of interest, BX (biodiesel content less and greater than in B10), were predicted in class 0. These results showed 100% correct classification of the training and test set samples for B10 and BX, demonstrating a high efficiency of the OPLS-DA model in the monitoring of crambe methyl biodiesel content when mixed with diesel in various proportions. The excellent results in the application of this model suggest that this analytical methodology is feasible, efficient and suitable for use by inspection agencies to control the quality of this fuel.

Chemical change during storage of crambe cultivated with application of plant growth regulators / Alterações químicas durante o armazenamento de crambe cultivado com aplicação de reguladores vegetais

This study aimed to characterize the chemical composition of crambe grains produced with plant growth regulators in the 2014 and 2015 harvests and stored for 180 days. During crambe cultivation, two foliar sprayings were carried out with the following treatments: 1) control treatment with distilled water; 2) indole-3-acetic acid 100 mg L-1 (IAA); 3) 3-gibberellic acid P.A. 100 mg L-1 (GA3); 4) Stimulate®. After harvesting, the grains were analyzed initially and after 180 days of uncontrolled storage. We checked water content, lipids in hot extraction (HE), lipids in cold extraction (CE), proteins, acidity and antioxidant activity. The Tukey test was used to compare the averages (p<0.05) and the parameters were grouped using a multivariate clustering technique presented in dendogram. The CE and HE lipid contents were reduced after storage, whereas the protein content and antioxidant activity of the grains were not altered by storage. However, the grains that received IAA application showed an increase in the CE lipid content and their preservation after storage. GA3 application, on the other hand, reduced the protein content. Lipid contents remained constant at the end of storage, and the acidity was reduced due to the application of GA3 and Stimulate® plant growth regulators. The 180-day storage changed crambe quality and regulators can assist in preserving grain quality during storage. The CE and HE lipid content and the grain acidity were good indicators for evaluating effects.

O objetivo do presente trabalho foi caracterizar a composição química dos grãos de crambe produzidos com reguladores vegetais nas safras de 2014 e 2015 e armazenados por 180 dias. Durante o cultivo do crambe, realizaram-se duas pulverizações foliares seguindo os tratamentos: 1) tratamento controle com água destilada; 2) ácido indol-3-acético 100 mg L-1 (AIA); 3) ácido 3-giberélico P.A. 100 mg L-1 (GA3); 4) Stimulate®. Após colhidos, os grãos foram analisados inicialmente e após 180 dias de armazenamento não controlado. Aferiu-se o teor de água, lipídeos em extração a quente (EQ), lipídeos em extração a frio (EF), proteínas, acidez e atividade antioxidante. As médias foram comparadas pelo teste Tukey (p<0,05) e os parâmetros agrupados pela técnica multivariada de agrupamento apresentado em dendograma. Os teores de lipídeos EF e EQ reduziram com o armazenamento enquanto que o teor de proteína e a atividade antioxidante dos grãos não foram alterados por esse fator. A aplicação de IAA destacou-se pelo aumento no teor de lipídeos EF e conservação desse após o armazenamento. A aplicação de GA3 reduziu o teor de proteínas. Os teores de lipídeos se mantiveram constante no final do armazenamento e houve redução no teor de acidez em decorrência da aplicação dos reguladores vegetais GA3 e Stimulate®. O armazenamento de 180 dias altera a qualidade de crambe e os reguladores podem auxiliar na preservação da qualidade dos grãos durante o armazenamento. O teor de lipídeos EF, EQ e a acidez dos grãos mostraram-se bons indicadores para avaliação dos efeitos.

Intake, digestibility and recovery of grains from feces of steers fed different oilseeds / Consumo, digestibilidade e recuperação de grãos nas fezes, de novilhos alimentados com diferentes grãos de oleaginosas

Oilseed grains are used as a protein and energy source, increasing the energy density of the feed. The goal of the present study was to determine the nutritional profile of canola, crambe and soybean grains recovered from feces, and to evaluate the intake and digestibility of nutrients, rumen fermentation parameters of beef cattle receiving whole grains of oilseeds. Three steers, 350 ± 23.5 kg body weight were distributed in a not contemporary, 3 × 3 double Latin square, and fed oilseed grains. Inclusion of crambe grain reduced dry matter intake by 21.02%, with a higher digestibility coefficient for EE and NDF. The crude protein content of canola and crambe grains recovered were similar, but the EE content was higher for the crambe grain (26.46%). Soybean showed the lowest values of chemical composition for grains recovered from feces. The highest dry matter intake (7.86 kg/day), and the lowest NDF digestibility (0.203g/kg), were found for animals fed soybean. The inclusion of crambe grains reduced the rumen ammonia content (10.97 mg/dL); however, the ruminal pH was not altered by the inclusion of grains in the diet. Canola and soybean grains can be included in the cattle diet without altering dry matter intake; while the inclusion of crambe grains reduces the dry matter intake and the ruminal ammonia content of the animals. Crambe grain showed the highest recovery of dry matter and ether extract from feces.(AU)

Para se avaliar a inclusão de grãos inteiros das culturas de canola, crambe e soja na dieta de bovinos em confinamento, sobre o consumo, digestibilidade, parâmetros ruminais e recuperação dos grãos nas fezes, delineou-se um ensaio estatístico em quadrado latino com três dietas e três bovinos providos de cânula ruminal. Os resultados constataram que a inclusão do grão de crambe reduziu o consumo de matéria seca em 21,02%, e proporcionou aumento na digestibilidade do extrato etéreo e fibra em detergente neutra. O percentual de extrato etéreo recuperado nas fezes foi de 44,15% para grão de canola e 61,53% para o grão de crambe. Encontrou-se menor digestibilidade da fibra em detergente neutra para dietas contendo grão de soja 0,203g/kg, embora seu consumo aumentou em relação ao crambe 7,86 kg/dia. A inclusão de crambe apresentou redução nos teores de amônia ruminal (N-NH3), com valores médios de 10,97 mg/dL. Portanto, o grão de crambe pode ser incluído na dieta de bovinos de corte em 14% da MS, sendo uma alternativa em potencial como grão de oleaginosa na alimentação de ruminantes. Assim como, os grãos de canola e soja sem afetar a digestibilidade da matéria seca e da proteína bruta, contudo, devem ser usados com cautela.(AU)

Preparation of Polyurethane Monolithic Resins and Modification with a Condensed Tannin-Yielding Self-Healing Property.

Resins of polyurethane were prepared from vegetable oils (crambe and castor) and modified by adding green corrosion inhibitor (condensed tannin). The oils were characterized by gas chromatography with flame-ionization detection (GC-FID), attenuated total reflectance Fourier transform infrared spectroscopy (FTIR-ATR) and thermogravimetric analysis (TGA). The reaction was monitored by characterizing the intermediate products (polyester and prepolymer). The polyester was characterized by solubility in methanol, acidity index, hydroxyl groups and FTIR-ATR, and the prepolymer was characterized by solid content, solvent content, isocyanate (NCO) groups and FTIR-ATR. The formation of PU resins was confirmed by FTIR-ATR and TGA, and the presence of tannin particles incorporated in the coating can be observed by optical microscopy (OM). The absence of the band attributed to NCO in FTIR-ATR spectra of the resins confirmed the complete reaction between polyester and prepolymer. The OM observation and a video demonstrate that Polyurethane (PU)-modified with condensed tannin resin presents self-healing effect, probably through the formation of new hydrogen bonds when in contact with deionized water. Therefore, these results open possibilities for new synthetic routes aiming at improving the very important self-healing property for protecting metals and their alloys against corrosion, extending significantly the metallic materials lifetime as previously demonstrated by our group.

Phytochemical screening and toxicity of Crambe abyssinica Hochst extracts on Solanum lycopersicum L., Euphorbia heterophylla L., Bidens pilosa L. and Glycine max (L. ) Merril / Screening fitoquímico e toxicidade de extratos de crambe abyssinica Hochst sobre Solanum lycopersicum L., Euphorbia heterophylla L., Bidens pilosa L. E Glycine max (L.) Merril

This study aimed to identify the main groups of secondary compounds from Crambe abyssinica and evaluate the bioactivity of the hexane, ethyl acetate and methanol extracts on the seed germination and seedling development of tomato, wild poinsettia, hairy beggartick and soybean. The phytochemical screening considered the presence or absence of total saponins, triterpenoids, flavonoids, coumarins, tannins, phenols and alkaloids. In the seeds it was evaluated: germination percentage, germination velocity index, average germination time, index of allelopathic effects, shoot and root length and seedlings dry matter. In the phytochemical screening it was observed that each solvent extracted different compounds. Flavonoids were found only in the ethyl acetate extract and saponin only in the methanol extract. A high allelopathic effect of hexane, ethyl acetate and methanolic extracts of crambe on the bio-indicator species tomato was observed. The hexane and ethyl acetate extracts also showed inhibitory effect on the weed hairy beggartick and did not present negative effects on soybean. There is the possibility of isolating the bio-active compounds of crambe and use them as a bio-herbicide to the alternative control of the weed hairy beggartick.

Este trabalho objetivouidentificar grupos de compostos do metabolismo secundário de Crambe abyssinica e avaliar a bioatividade dos extratos hexânico, acetato etílico e metanólico 1% sobre a germinação de sementes e o crescimento de plântulas de tomate, leiteiro, picão-preto e soja. O screeningfitoquímico considerou a presença ou ausência de saponinas totais, triterpenóides, esteroides, flavonoides, cumarinas, taninos, fenóis e alcaloides. Nas sementes avaliou-se a porcentagem de germinação, índice de velocidade de germinação, tempo médio de germinação, índice de resposta do efeito alelopático, comprimento de parte aérea e raiz e massa seca das plântulas.No screening fitoquímico observou-se que cada solvente extraiu compostos diferentes. Flavonóides foram encontrados somente no extrato acetato etílico e saponina apenas no extrato metanólico. Verificou-se elevado efeito alelopático dos extratos hexânico, acetato de etila e metanólico de crambe sobre a espécie bio-indicadora tomate. Os extratos hexânico e acetato etílico também apresentaram efeito inibitório sobre a planta invasora picão-preto e não tiveram efeitos negativos sobre a soja. Existe a possibilidade de isolamento de compostos bioativos de crambe para utilização como possível bio-herbicida para controle alternativo da planta invasora picão-preto.

Qualidade fisiológica de sementes de crambe tratadas com zinco e molibdênio / Physiological quality seeds crambe treated with zinc and molybdenum

A cultura do crambe vem ganhando importância no Brasil devido ao estímulo à produção e uso de biodiesel. E a aplicação de micronutrientes via tratamento de sementes visando aumentar a produtividade tem apresentado resultados positivos na agricultura. No entanto, pouco se sabe sobre o real efeito desses produtos na qualidade fisiológica de sementes de crambe. Desta forma, o objetivo do trabalho foi avaliar a qualidade fisiológica de sementes de Crambe abyssinica Hochst tratadas com fertilizante líquido a base de zinco e molibdênio. O experimento foi conduzido no Laboratório de Análise de Sementes da Unimontes. O delineamento experimental utilizado foi inteiramente casualizado, com quatro repetições. Os tratamentos testados constituíram de cinco doses de fertilizante comercial composto por zinco (35%) e molibdênio (3,5%), aplicados via semente nas concentrações de 0; 3,3; 6,7; 10 e 13mLdo fertilizante para 0,5 Kg-¹ de sementes. A qualidade fisiológica das sementes foi avaliada por meio dos seguintes testes: germinação, primeira contagem de germinação, emergência e comprimento de plântulas. Com o aumento das doses do fertilizante comercial composto por zinco e molibdênio, houve redução na germinação e vigor das sementes de crambe. A qualidade fisiológica das sementes de crambe é influenciada negativamente por doses crescentes dezinco e molibdênio.(AU)

The crambe culture has been gaining importance in Brazil due to the stimulus to the production and use of biodiesel. And the application of micronutrients via seed treatment has shown positive results in agriculture. However, little is known about the actual effect of these products on the physiological quality of crambe seeds. Thus, the objective of this work was to evaluate the physiological quality of Crambe abyssinica Hochst seeds treated with liquid fertilizer based on zinc and molybdenum. The experiment was conducted at the Unimontes Seed Analysis Laboratory. The experimental design was completely randomized, with four replications. The treatments tested consisted of five doses of commercial fertilizer composed of zinc (35%) and molybdenum (3.5%), applied via seed at concentrations of 0; 3.3; 6.7; 10 and 13 mL of the fertilizer to 0.5 kg-¹of seeds. The physiological quality of the seeds was evaluated by means of the following tests: germination, first germination count, emergence and seedling length. With increasing doses of commercial fertilizer composed of zinc and molybdenum, there was a reduction in germination and vigor of crambe seeds. The physiological quality of crambe seeds is negatively influenced by increasing doses of zinc and molybdenum.(AU)

Qualidade fisiológica de sementes de crambe tratadas com zinco e molibdênio / Physiological quality seeds crambe treated with zinc and molybdenum

A cultura do crambe vem ganhando importância no Brasil devido ao estímulo à produção e uso de biodiesel. E a aplicação de micronutrientes via tratamento de sementes visando aumentar a produtividade tem apresentado resultados positivos na agricultura. No entanto, pouco se sabe sobre o real efeito desses produtos na qualidade fisiológica de sementes de crambe. Desta forma, o objetivo do trabalho foi avaliar a qualidade fisiológica de sementes de Crambe abyssinica Hochst tratadas com fertilizante líquido a base de zinco e molibdênio. O experimento foi conduzido no Laboratório de Análise de Sementes da Unimontes. O delineamento experimental utilizado foi inteiramente casualizado, com quatro repetições. Os tratamentos testados constituíram de cinco doses de fertilizante comercial composto por zinco (35%) e molibdênio (3,5%), aplicados via semente nas concentrações de 0; 3,3; 6,7; 10 e 13mLdo fertilizante para 0,5 Kg-¹ de sementes. A qualidade fisiológica das sementes foi avaliada por meio dos seguintes testes: germinação, primeira contagem de germinação, emergência e comprimento de plântulas. Com o aumento das doses do fertilizante comercial composto por zinco e molibdênio, houve redução na germinação e vigor das sementes de crambe. A qualidade fisiológica das sementes de crambe é influenciada negativamente por doses crescentes dezinco e molibdênio.

The crambe culture has been gaining importance in Brazil due to the stimulus to the production and use of biodiesel. And the application of micronutrients via seed treatment has shown positive results in agriculture. However, little is known about the actual effect of these products on the physiological quality of crambe seeds. Thus, the objective of this work was to evaluate the physiological quality of Crambe abyssinica Hochst seeds treated with liquid fertilizer based on zinc and molybdenum. The experiment was conducted at the Unimontes Seed Analysis Laboratory. The experimental design was completely randomized, with four replications. The treatments tested consisted of five doses of commercial fertilizer composed of zinc (35%) and molybdenum (3.5%), applied via seed at concentrations of 0; 3.3; 6.7; 10 and 13 mL of the fertilizer to 0.5 kg-¹of seeds. The physiological quality of the seeds was evaluated by means of the following tests: germination, first germination count, emergence and seedling length. With increasing doses of commercial fertilizer composed of zinc and molybdenum, there was a reduction in germination and vigor of crambe seeds. The physiological quality of crambe seeds is negatively influenced by increasing doses of zinc and molybdenum.

Foliar application of plant growth regulators changes the physiological quality of crambe seeds

The application of plant growth regulators can manipulate the development of cultivated plants, and their physiological effects may be reflected in the physiological quality of the seeds. This study evaluated the effects of plant growth regulators (PGR) on the physiological quality of crambe seeds produced in the 2014 and 2015 harvests. During crop development, we applied two foliar sprayings of the following treatments: 1) control treatment with distilled water; 2) indole-3-acetic acid 100 mg L-1 (IAA); 3) 3-gibberellic acid P.A. 100 mg L-1 (GA3); 4) commercial PGR Stimulate® 6 mL L-1. The seeds were harvested and evaluated for water content, germination percentage and germination speed index (GSI), electrical conductivity and concentrations of leached Ca, Mg and K ions. Means were compared by Tukey ́s and Dunnett tests (p<0.05). Applying Stimulate® increased the germination percentage and the germination speed index, while IAA application showed an opposite effect, increasing the electrical conductivity of crambe seeds. Calcium leaching decreased with IAA application, and all regulators reduced K leaching. We therefore infer that the application of plant growth regulators influences the physiological quality of crambe seeds.

Sorghum silage supplemented with crambe meal improves dry matter intake and milk production in crossbred Holstein cows.

We studied the feed intake and milk production of Holstein × Zebu crossbred cows fed sorghum silage diets and concentrates, with and without the addition of crambe meal. Using a change-over design for a total of 120 days, eight cows were fed two diets (concentrates + silages with and without crambe meal) in four 30-day periods, with four replications (animals). The crambe meal diet increased (P < 0.05) the dry matter intake (DMI), crude protein intake (CPI), and non-fibrous carbohydrates intake (NFCI) as well as milk production (4.3%). No difference was noted (P > 0.05) for the milk composition between the treatments. Crambe meal as an additive in sorghum silage (100 g/kg in natural matter) showed a great potential for introduction in dairy farming as it substantially increases CP intake, does not reduce food intake, raises animal productivity, and does not affect milk composition.

Intake, digestibility, performance and carcass characteristics of ewes fed crambe replacing soybean meal in the diet / Consumo, digestibilidade, desempenho e características de carcaça de ovelhas alimentadascom crambe em substituição à farelo de soja

The use of alternative sources of protein for ruminant can alter productivity and reducecosts in animal production. The aim of this study was to evaluate the performance, apparent digestibilityand quantitative characteristics of carcass components of ewes fed increasing levels (0, 50, 100 and 150 g kg1 dry matter) of crambe crushed in the diets. Twenty-four culling ewes were distributed in a completelyrandomized design. All animals were kept in a shed, with individual stalls equipped with feeder, drinkerand concrete floor covered with rice hulls. The animals received corn silage as roughage and diets wereisoenergetic with 700 g kg-1 of TDN. Animals were slaughtered when reached a body condition score of3.5 points. The dry matter intake (948.53 g day-1) and average daily gain (0.15 kg day-1) were not influencedby the inclusion of crambe crushed in the diet. The crambe crushed increased ether extract digestibility anddecreased crude protein intake and digestibility. There was no effect of the levels of crambe crushed oncarcass characteristics and non-carcass components but rather a reduction in the cold carcass yield. Totalreplacement of soybean meal with crambe crushed in the diet for ewes did not alter performance, carcassand non-carcass characteristics.

O uso de fontes proteicas alternativas para ruminantes pode alterar a produtividade e reduzir oscustos na produção animal. O objetivo da pesquisa foi avaliar o desempenho, a digestibilidade aparente e ascaracterísticas quantitativas dos componentes de carcaça das ovelhas alimentadas com níveis crescentes (0,50, 100 e 150 g kg-1 de matéria seca) de torta de crambe nas dietas. Foram distribuídas 24 ovelhas em umdelineamento inteiramente casualizado. Todos os animais foram mantidos em instalação, com baiasindividuais equipadas com cocho, bebedouro e chão de concreto coberto com cascas de arroz. Os animaisreceberam silagem de milho como forragem e as dietas foram isoenergéticas com 700 g kg-1 de NDT. Osanimais foram abatidos quando atingiram o escore de condição corporal de 3,5 pontos. A ingestão dematéria seca (948,53 g dia-1) e o ganho médio diário (0,15 kg dia-1) não foram influenciados pela inclusão detorta de crambe na dieta. A torta de crambe aumentou a digestibilidade do extrato etéreo e diminuiu aingestão e digestibilidade da proteína bruta. Não houve efeito da torta de crambe nas características dacarcaça e nos componentes não carcaça, no entanto houve redução no rendimento da carcaça fria. Asubstituição total da farinha de soja e a torta de crambe na dieta para as ovelhas não alteraram ascaracterísticas de desempenho, carcaça e não carcaça.(AU)

Intake, digestibility, performance and carcass characteristics of ewes fed crambe replacing soybean meal in the diet / Consumo, digestibilidade, desempenho e características de carcaça de ovelhas alimentadascom crambe em substituição à farelo de soja

The use of alternative sources of protein for ruminant can alter productivity and reducecosts in animal production. The aim of this study was to evaluate the performance, apparent digestibilityand quantitative characteristics of carcass components of ewes fed increasing levels (0, 50, 100 and 150 g kg1 dry matter) of crambe crushed in the diets. Twenty-four culling ewes were distributed in a completelyrandomized design. All animals were kept in a shed, with individual stalls equipped with feeder, drinkerand concrete floor covered with rice hulls. The animals received corn silage as roughage and diets wereisoenergetic with 700 g kg-1 of TDN. Animals were slaughtered when reached a body condition score of3.5 points. The dry matter intake (948.53 g day-1) and average daily gain (0.15 kg day-1) were not influencedby the inclusion of crambe crushed in the diet. The crambe crushed increased ether extract digestibility anddecreased crude protein intake and digestibility. There was no effect of the levels of crambe crushed oncarcass characteristics and non-carcass components but rather a reduction in the cold carcass yield. Totalreplacement of soybean meal with crambe crushed in the diet for ewes did not alter performance, carcassand non-carcass characteristics.

O uso de fontes proteicas alternativas para ruminantes pode alterar a produtividade e reduzir oscustos na produção animal. O objetivo da pesquisa foi avaliar o desempenho, a digestibilidade aparente e ascaracterísticas quantitativas dos componentes de carcaça das ovelhas alimentadas com níveis crescentes (0,50, 100 e 150 g kg-1 de matéria seca) de torta de crambe nas dietas. Foram distribuídas 24 ovelhas em umdelineamento inteiramente casualizado. Todos os animais foram mantidos em instalação, com baiasindividuais equipadas com cocho, bebedouro e chão de concreto coberto com cascas de arroz. Os animaisreceberam silagem de milho como forragem e as dietas foram isoenergéticas com 700 g kg-1 de NDT. Osanimais foram abatidos quando atingiram o escore de condição corporal de 3,5 pontos. A ingestão dematéria seca (948,53 g dia-1) e o ganho médio diário (0,15 kg dia-1) não foram influenciados pela inclusão detorta de crambe na dieta. A torta de crambe aumentou a digestibilidade do extrato etéreo e diminuiu aingestão e digestibilidade da proteína bruta. Não houve efeito da torta de crambe nas características dacarcaça e nos componentes não carcaça, no entanto houve redução no rendimento da carcaça fria. Asubstituição total da farinha de soja e a torta de crambe na dieta para as ovelhas não alteraram ascaracterísticas de desempenho, carcaça e não carcaça.

The crambe (Crambe abyssinica Hochst) byproducts, can be used as a source of non-degradable protein in the rumen? / Coprodutos de crambe (Crambe abyssinica Hochst) podem ser utilizados como fonte de proteina não degrádavel no rúmen?

To evaluated the chemical composition and ruminal degradability of crambe byproducts (meal and crushed) and proteic supplements formulated with crushed crambe (0; 2.5; 5; 10 and 15%); five crossbred steers with average weight of 485±14 kg, were used. All the animal were kept in individual paddocks of 0.25 ha on Urochloa brizantha (syn. Brachiaria brizantha). It was observed a greater soluble fraction, higher effective degradability at 5%/h and higher degradation rate "c" and, consequent, lower indigestible fraction for crambe crushed ground in the sieve of 3 mm. The effective degradability at 5%/h was lower for the crambe crushed (55.42) in relation to the meal (48.80). The diet with 5% of inclusion of crambe showed higher effective degradability for dry matter (54.86%) and lower fraction "I" (30.64%) associated with higher fractions "c" and "b". Crambe ground in sieves of 1 and 3 mm mesh presented the highest degradability. Crushed crambe showed higher ruminal degradation than crambe meal; the crambe byproducts possibility can be use as a source of non-degradable protein in the rumen.

Para se avaliar a composição química, degradabilidade ruminal, o tempo da colonização da torta e do farelo de crambe e de suplementos protéico compostos de torta de crambe (0; 2,5; 5,0; 10 e 15%); foram utilizados cinco novilhos mestiços, com peso médio de 485 ± 14 kg. Todos os animais foram mantidos em piquetes individuais de 0,25 ha em pastagens de Urochloa brizantha (syn. Brachiaria brizantha). Observou-se uma fração solúvel maior, maior degradabilidade efetiva para a taxa de passagem de 5% / h e maior taxa de degradação "c" e, consequentemente, menor fração indigerível para a torta de crambe moído na peneira de 3 mm. A degradabilidade efetiva a 5% / h foi menor para a torta de crambe (55,42) em relação ao farelo (48,80). A dieta com adição de 5% de torta de crambe apresentou maior degradabilidade efetiva da matéria seca (54,86%) e menor fração "I" (30,64%), associada com as frações mais elevadas "c" e "b". A torta de Crambe moída em peneiras de 1 e 3 mm de diâmetro apresentaram os maiores valores de degradabilidade. A torta de apresenta maior degradação ruminal que o farelo de crambe. Os coprodutos de crambe possivelmente podem ser usados como fonte de proteína não degradada no rúmen.