Avaliação do efeito antioxidante da bebida de café solúvel cafeinado e descafeinado In vitro e In vivo / Antioxidant effect evaluation of caffeinated and decaffeinated coffee brew In vitro and In vivo

Fitoquímicos com ação antioxidante presentes no café, apresentam diversos benefícios na saúde devido as suas propriedades funcionais. A atividade antioxidante foi avaliada utilizando-se ensaios in vitro para se investigar a atividade sequestrante de radicais livres DPPH e testes in vivo para determinar a inibição da peroxidação lipídica. Os dados obtidos permitem sugerir que as bebidas de café solúveis cafeinado e descafeinado apresentaram uma forte atividade antioxidante e esta é dependente da concentração. A atividade antioxidante in vitro da bebida de café solúvel cafeinado apresentouse maior do que a do café solúvel descafeinado. No entanto, o tratamento não inibiu a peroxidação lipídica do cérebro de ratos in vivo, em comparação com o controle. O tratamento com a ingestão das diferentes bebidas reduziu a concentração de ferro sérico. Os dados obtidos sugerem que as bebidas de café solúvel apresentam uma forte atividade antioxidante e esta é dependente da concentração.

Phytochemicals with antioxidant activity contained in coffee presents many health benefits due to their functional properties. This study aimed to determine the content of phenolic compounds and the antioxidant activity of soluble caffeinated and decaffeinated coffee beverage. Soluble solid parameters and phenolic compounds, as well as, antioxidant activity were analyzed using in vitro essays to investigate free radical scavenging activity. In vivo essays were used to determine lipid peroxidation inhibition. The in vitro antioxidant activity of soluble caffeinated coffee was higher comparing to decaffeinated soluble coffee. However, comparing to the control, the treatment does not inhibit rat brain lipid peroxidation in vivo. It was also observed that the consumption of different beverages reduces the concentration of serum iron. The data obtained suggest that soluble coffee beverages present a strong antioxidant activity which depends on the concentration.

The inclusion of coffee in commercial layer diets

This experiment aimed at evaluating the effect of the dietary inclusion of caffeinated and decaffeinated coffee on the performance and internal and external egg quality of commercial layers. One hundred and twenty 25-week-old Hy-line Brown layers, with 1575 ± 91 average body weight, were distributed according to a completely randomized experimental design with three treatments (control, 1.2% caffeinated coffee, or 1.2% decaffeinated coffee) of five replicates of eight birds each. The inclusion of 1.2% caffeinated coffee was calculated to supply 6mg caffeine per kg body weight, which is considered a moderate dose. The applied treatments did not influence (p>0.05) feed intake, egg production, egg weight, egg mass, feed conversion ratio, Haugh units, yolk color or albumen and yolk percentages. The eggs of hens fed 1.2% caffeinated coffee presented lower (p<0.05) eggshell thickness and egg specific density. The eggs of layers fed 1.2% caffeinated coffee tended (p=0.0637) to present lower eggshell percentage. It was concluded that feeding caffeinated coffee to commercial layers does not affect their performance or internal egg quality; however, eggshell quality is impaired.(AU)

The inclusion of coffee in commercial layer diets

This experiment aimed at evaluating the effect of the dietary inclusion of caffeinated and decaffeinated coffee on the performance and internal and external egg quality of commercial layers. One hundred and twenty 25-week-old Hy-line Brown layers, with 1575 ± 91 average body weight, were distributed according to a completely randomized experimental design with three treatments (control, 1.2% caffeinated coffee, or 1.2% decaffeinated coffee) of five replicates of eight birds each. The inclusion of 1.2% caffeinated coffee was calculated to supply 6mg caffeine per kg body weight, which is considered a moderate dose. The applied treatments did not influence (p>0.05) feed intake, egg production, egg weight, egg mass, feed conversion ratio, Haugh units, yolk color or albumen and yolk percentages. The eggs of hens fed 1.2% caffeinated coffee presented lower (p 0.05) eggshell thickness and egg specific density. The eggs of layers fed 1.2% caffeinated coffee tended (p=0.0637) to present lower eggshell percentage. It was concluded that feeding caffeinated coffee to commercial layers does not affect their performance or internal egg quality; however, eggshell quality is impaired.