Correcting bias of creatinine testing using Jaffe assay by double-levels calibration / 临床检验杂志

Objective To evaluate the bias of creatinine testing by external quality assessment (EQA) and analyze the effects of calibration on the bias of tested values using Jaffe assay.Methods The EQA data from The Clinical Laboratory Centre of Chongqing City were utilized to investigate the frozen pooled-human serum in which the value of creatinine was determined by reference method.The differences between Jaffe assay and creatine oxidase method were analyzed and the bias was evaluated.After the routine measurement systems were calibrated using the frozen pooled-human sera with double levels,the EQA samples were tested.Results The number of the laboratories using Jaffe method for creatinine measurement reduced significantly in Chongqing from 2012 to 2016.The robust average values of Jaffe assay were different from enzymatic assay significantly and the difference of the values were correlated with the concentration of creatinine (r =0.774 6).The results of the investigation using frozen pooled human serum and the trueness verification program of National Center for Clinical Laboratories indicated the positive bias of Jaffe assay was observed in low levels of creatinine with maximum of 10.39％ and 13.31％ and the bias correlated with the concentration of creatinine (r =0.988 9),by contrast the bias was low in enzymatic assay with maximum of 1.04％ and 1.49％ only.After the measurement systems were calibrated by frozen pooled human sera with double-levels,the positive bias of Jaffe assay in the low concentration of creatinine could be corrected.Conclusion There may be a positive bias of Jaffe assay for the samples with low concentration of creatinine,which could be corrected by using the calibrators with double-levels.

Symptoms and Signs for the Syndrome of Qi-deficiency,Blood-deficiency,Yin-deficiency and Yang-deficiency / 浙江中医药大学学报

Syndrome factors as refined diagnostic units are the base of syndrome differentiation,each syndrome factor has the relative symptom and sign.By way of epidemiology and documents investigation,as well as algorithm of double levels of frequency and weight,the symptom and sign for the syndrome factor of Qi-deficiency,Blood-deficiency,Yin-deficiency and Yang-deficiency respectively were extracted correctly,and the weight values of symptoms and signs to syndrome factors were established.