RESUMO
This study aimed to investigate the chemical constituents of supercritical extract from Qi-nan Aquilariae Lignum Resinatum by silica gel column chromatography, thin-layer chromatography, and semi-preparative high-performance liquid chromatography. One new elemane-type and one new eudesmane-type sesquiterpene compounds were isolated from the extract, and their structures were identified by MS, UV, IR, NMR, and ECD spectroscopic techniques, and named aquqinanol C(1) and aquqinanol D(2). Both compounds are novel compounds. The neuroprotective effect of the compounds on CORT-induced PC12 cell damage was determined in vitro. The results showed that compounds 1 and 2 exhibited a certain protective effect against CORT-induced damage in PC12 cells.
Assuntos
Qi , Sesquiterpenos , Ratos , Animais , Sesquiterpenos/farmacologia , Estrutura MolecularRESUMO
This study aimed to investigate the chemical constituents of supercritical extract from Qi-nan Aquilariae Lignum Resinatum by silica gel column chromatography, thin-layer chromatography, and semi-preparative high-performance liquid chromatography. One new elemane-type and one new eudesmane-type sesquiterpene compounds were isolated from the extract, and their structures were identified by MS, UV, IR, NMR, and ECD spectroscopic techniques, and named aquqinanol C(1) and aquqinanol D(2). Both compounds are novel compounds. The neuroprotective effect of the compounds on CORT-induced PC12 cell damage was determined in vitro. The results showed that compounds 1 and 2 exhibited a certain protective effect against CORT-induced damage in PC12 cells.
Assuntos
Ratos , Animais , Qi , Sesquiterpenos/farmacologia , Estrutura MolecularRESUMO
Six compounds were isolated from the aerial part of cultivated Clerodendranthus spicatus in Hainan with various chromatographic techniques,and their structures were determined as:1-dehydroxy-1-oxo-rupestrinol(1),N-trans-feruloyltyramine(2),methyl 3,4-dihydroxyphenyllactate(3),caffein acid(4),methyl caffeate(5) and ethyl caffeate(6),via analysis of physicochemical properties and spectroscopic evidence.Compound 1 was a new compound,while compounds 2 and 3 were isolated from C.spicatus for the first time.Biological activity results showed that compounds 2-4 exhibited α-glucosidase inhibitory activity with different inhibition ratio.
Assuntos
Inibidores de Glicosídeo Hidrolases/farmacologia , Lamiaceae/química , Sesquiterpenos de Eudesmano/farmacologia , China , Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Sesquiterpenos de Eudesmano/isolamento & purificaçãoRESUMO
Six compounds were isolated from the aerial part of cultivated Clerodendranthus spicatus in Hainan with various chromatographic techniques,and their structures were determined as:1-dehydroxy-1-oxo-rupestrinol(1),N-trans-feruloyltyramine(2),methyl 3,4-dihydroxyphenyllactate(3),caffein acid(4),methyl caffeate(5) and ethyl caffeate(6),via analysis of physicochemical properties and spectroscopic evidence.Compound 1 was a new compound,while compounds 2 and 3 were isolated from C.spicatus for the first time.Biological activity results showed that compounds 2-4 exhibited α-glucosidase inhibitory activity with different inhibition ratio.
Assuntos
China , Inibidores de Glicosídeo Hidrolases , Farmacologia , Lamiaceae , Química , Estrutura Molecular , Compostos Fitoquímicos , Farmacologia , Sesquiterpenos de Eudesmano , FarmacologiaRESUMO
Eudesmane-type sesquiterpenes have been reported to exhibit varieties of biological activities. During the process of investigating this kind of natural product from the root bark of Dictamnus dasycarpus Turcz., 13 eudesmane-type sesquiterpene glycosides including six new isolates, named as dictameudesmnosides A1 (1), A2 (2), B (3), C (4), D (5), and E (6), together with seven known ones (7-13), were obtained. Herein, their structures were determined by the analysis of physical data, spectroscopic analysis, and chemical methods. The existence of α-configuration glucose units in their structures (1-5, 8) is not very common in natural glycosidic components. Meanwhile, compounds 3-5, 7, and 9-13 displayed TG accumulation inhibitory effects on HepG2 cells.
Assuntos
Dictamnus/química , Glicosídeos/isolamento & purificação , Sesquiterpenos de Eudesmano/isolamento & purificação , Proliferação de Células/efeitos dos fármacos , Glicosídeos/química , Glicosídeos/farmacologia , Células Hep G2 , Humanos , Estrutura Molecular , Casca de Planta/química , Sesquiterpenos de Eudesmano/química , Sesquiterpenos de Eudesmano/farmacologiaRESUMO
A new sesquiterpene manghookerins A (1) was isolated from the barks of Manglietia hookeri (Magnoliaceae) together with five known eudesmane-type sesquiterpenes. The structure of manghookerins A (1) was elucidated by physical and spectroscopic data analysis, including 1D and 2D NMR, HR-ESI-MS and X-ray diffraction. The structures of known sesquiterpenes were established by comparing their spectroscopic data with those in literatures.
Assuntos
Magnoliaceae/química , Sesquiterpenos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Casca de Planta/química , Sesquiterpenos/química , Difração de Raios XRESUMO
Two new eudesmane-type glucopyranosides have been isolated from the fruits of Daucus carota L. On the basis of their spectroscopic and chemical evidence, the new compounds were elucidated as daucucarotol-10-O-ß-D-glucopyranoside (1) and decahydro-7-[(2-O-ß-D-glucopyranosyl)-isopropyl]-1ß,4aα-dimethyl-(1α,4α,8aß)-naphthalenetriol (2). Compounds 1 and 2 showed moderate antitumour activity against human ECA-109 and gave IC50 values of 23.22 and 26.76 µM, respectively.
