RESUMO
Objective To study the method of extraction,purification,and analysis of total flavonoids of Radix Glycyrrhizae(TFG).Methods The extracting effects of three different ways(ultrasonic wave,heating recirculation,and maceration)for TFG were compared.TFG was refined with resin absorption and the effects of different parameters on yield and purity were observed.Naringin scanning method was used to analyze TFG.Results TFG extracting percentage is 5.46 %by ultrasonic wave method,6.12 %by heating recirculation,and 5.04 %by maceration,the effect of heating recirculation being the best.In the test of resin absorption of TFG,the heating recirculation composition is mainly located in 40 %～60 %ethanol elution parts.Conclusion The best method for extracting TFG is heating recirculation in above three methods,Elution with 80%ethanol continuously after water elution is the the optimal resin purification method for TFG.
RESUMO
The effect of Flavonoids of Gly-cyrrhiza (FG) on ethanol-induced liver injury was studied in mice. Administration of FG to mice prior to ethanol treatment ,either once daily for four days or a single dose, the increase of malondialdehyde and the decrease of reduced glutathione were inhibited dose- dependently.Electron microscopic examination showed that the ethanol-induced damage of hepatocytes could be ameliorated by FG pretreatment. However, no protective effect was observed when FG was administrated to mice after ethanol intoxication.
